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Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1

 

Life Sciences International Journal Issue 7 Volume 1
...f circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studi...

Keith Ward

 

Science, Religion and Culture
...e game away on p. 228 by saying, ‘we human beings ...are ourselves mere collections of fundamental particles’. That is a philosophical view if ever there was one! It is precisely what is usually called eliminative reductionism, and I regard it as a non-starter, if you want to give a complete explanation of the universe.

 

...

Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad

 

British Journal of Virology
...f circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studi...

Robert E. Pollack

 

Science, Religion and Culture
...go but including some passages from the Hebrew bible’s Torah, Prophets and Writings that are millennia old.

 

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Ruse, Michael, The Gaia Hypothesis: Science on a Pagan Planet, The University of Chicago Press, 2013, 251 pp, $26.00, ISBN 978-0-226-73170-4

 

Science, Religion and Culture

Tom Gilson & Carson Weitnauer (eds), True Reason: Confronting the Irrationality of the New Atheism (Grand Rapids, MI: Kregel Publications, 2013), 315pp, US$17.99 (paperback), ISBN 978-0-8254-4338-1

Reviewed by Gregory W. Dawes, Associate Professor of Philosophy

Science, Religion and Culture
...s of Christianity. Its essays cover an extraordinarily wide range of topics. These include the theological problems posed by religious diversity, the so-called “naturalism” of the modern sciences, the relations between faith and reason and religion and science, the role of religion in supporting our moral convictions, the reliability of the biblical accounts of Jesus’ life, and the problems posed by biblical endorsements of slavery and genoci...

Timothy Helton

In a Mirror Dimly: Anthropology and Restoring a Sense of Presence to an Empty World
...l sense.

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Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1

British Journal of Virology
...f circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studi...

Joseph Andrew Bracken

Metaphysics for a World in Evolution
...o the Many as their necessary principle of unity and intelligibility. I then propose a new paradigm for the relation between the One and the Many in which the Many by their dynamic interrelation from moment to moment initially co-create and then sustain the One not as a higher-order individual entity but as their conjoint energy-source and ongoing field of activity. The One can then be readily integrated into a hierarchical ordered system of fields within fiel...

Reviewed by John Shook, University at Buffalo, USA; Email: jshook@pragmatism.org

Science, Religion and Culture
...anities and sciences the same five ques- tions about science and religion, what would you ask? This opportunity came to philosopher Gregg D. Ca- ruso, thanks to a publisher whose “5 Questions” series is permitting readers to examine direct answers on all sorts of topics from today’s premier public intellectu- als. Reading the answers about science and religion in this volume is consistently informative and inspi- rational, and frequently reve...

Stephen Kershnar

...for example, Mother Theresa, Adolf Hitler, and Paul Gauguin), artists (for example, Aldous Huxley and Woody Allen), and works of art (for example, Groundhog Day and Guernica). In this critical study, I summarize the book and then put forth objections to its central ideas. In particular, I argue that Metz fails to show that there is meaning in life and that, even if there is, his theory likely fails to capture it.

...

Thaddeus Metz

...from and competing with, say, objective well-being, and, second, that, even if there were a value of meaning in life, my fundamentality theory of it would not capture its essence well. In this article, I reply to both of these criticisms, aiming to probe these underexplored issues still more deeply. I also contend that these two criticisms are in tension with each other; in order to contend that my theory of meaning is incorrect, Kershnar must draw on intuitio...

Sandeep Satapathy and Rupam Ghosh

...to cytoplasm and vice versa controlling the overexpression of IFN-beta and ISGs which subsequently triggers virus specific immune response.

...

Michael R. Molnar

...n the Star. The Star, he says, has no historical basis nor can it even be explained as a miracle.

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Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3

..., muco-purulent ocular-nasal discharges with matted eyelids and encrusted nostrils and finally death. Clinical outcome, gross lesions and histological observations were suggestive of PPRV infection, which were confirmed by the application of a reverse transcription–polymerase chain reaction (qRT-PCR). Collectively, results indicate the continuous persistence of PPRV in Kenya.

...

James Ayukepi Ayukekbong1,2

E-mail | james.ayukekbong@microbio.gu.se

... fever is therefore necessary to initiate the appropriate clinical care. Despite the endemicity of dengue in Nigeria, the disease is not a reportable disease and routine diagnosis is neglected. Here I undertake an exhaustive assembly of known records of dengue prevalence in Nigeria and provide a focus for public health intervention.

...

Gregg D. Caruso

...e’s actions are causally determined (ch.5) and develops a forward-looking theory of moral responsibility consistent with free will skepticism (ch.6). A significant contribution to the field, Free Will, Agency, and Meaning in Life is destined to become a classic and is essential reading for anyone interested in free will and moral responsibility.

...

Dana Kay Nelkin

...that we have it. At the same time, there are some subtle shifts in particular commitments, new ways of framing certain issues, and detailed responses to a good number of the very many challenges to the view as originally set out in Living Without Free Will and related articles. The responses to challenges serve to elaborate, extend, and deepen the view. The result is a book that, along with the first, will be a centerpiece of the conver

Derk Pereboom

...r for his generous appraisal of the book, and for his astute and challenging comments on my treatment of the manipulation argument in Chapter 4. Fischer’s core strategy for resisting this argument is (at least initially) a soft-line reply. Soft-liners claim that in some manipulation cases the agent is not morally responsible, and in others he is. A corollary of the soft-line reply is that there is a plausible compatibilist condition on moral responsibili...

Muhammad Arif1, Fazal Jalal1, Mohammad Tariq Jan1, Dost Muhammad2

...ber of grains ear-1, thousand grains weight, grain and biological yield. It is concluded that integration of biochar and legumes could be a useful strategy for enhancing the overall farm profitability and productivity of cereal-based systems by providing increased yields from this additional ‘summer gap’ crop.

...

Zaheer Ahmed Deho1, Shamsuddin Tunio2, Qammaruddin Chachar2, Fateh Chand Oad2

Shahid Ali, Munir Khan

...istage stratified random sampling technique during 2009-10. Stochastic frontier analysis was employed for the estimation of technical efficiency levels and its determinants. Maximum likelihood estimation (MLE) technique was applied to estimate stochastic frontier Cobb-Douglas production function to ascertain the level of technical efficiency. The econometric computer software STATA was applied for the estimation. The estimated value of technical efficiency ran...

Farhad Zulfiqar, Muhammad Ashfaq

...o wheat were used for Faisalabad and Ba- hawalpur. The results of analysis showed that the effect of climatic variables was found significantly higher than that of non-climatic variables. The largest impact on wheat yield was of mean maximum average temperature at the time of maturity, ceteris paribus with one oC increase in temperature the average wheat yield increased by 56.64 kg per hectare in the study area. It was deduced from the economic vari...

Mohammed Ali Hussain, Zakiya Ahmed Hassan

...mprise seven F1 hybrids (sangaria, IK8 x zp-707, DK x Hs, zp-707 x un 44052, IK 8 x un44052, un 44052 x DK and polina). The experiment was laid out in randomized complete block design with three replications. The analysis of variance showed highly significant differences among maize hybrids for all the traits. The hybrid un44052 x DK took minimum days to tasseling (65.83 days) and silking (68.50 days). Hybrid zp-707 x un 44052 was short statured (155.59 cm) wi...

Massimo Pigliucci, K.D.

... terrorist attack on the satirical paper Charlie Hebdo, which took place on 7 January 2015. Some of the commentary has been insightful, some full of pious platitudes about defense of free speech by sources with not exactly a stellar record in that department, and some of it has ranged from the woefully uninformed to the downright awful. It is, therefore, with some recalcitrance that I write these lines, particularly because I’m coming to the issue from w...

John Teehan

...ital questions of public safety and the costs we are willing to pay for that safety. They also raise questions of how to understand the complex conditions that give rise to such events and the conditions for such events are always complex, even when facile “explanations” are readily available, i.e. the violent nature of Islam, their hatred of Western freedom, etc. A consistent element in many such explanations is...

Michael Shermer

...Pop quiz: What did the assailants shout after murder- ing 17 people in Paris in January, 2015, including edi- tors and cartoonists of the satirical publication Charlie Hebdo?


A. “Moses rocks!”
B. “Jesus saves!”
C. “Vishnu lives!”
D. “Buddha avenged!” E. “Atheists rule!”
F. “Allahu ...

Frank Griffel

... acts of terrorism is to say: We have done nothing wrong. In fact, the collective wearing of signs with “Je suis Charlie” (“I am Charlie”) says: We would do it all over again. Last week, almost a whole nation lined up at French newsstands to buy the first issue of “Charlie Hebdo” after the attacks on the satirical magazine. It showed yet another caricatu...

Muhammad Mushtaq

E-mail | mushtaq@uaar.edu.pk

...and supplemented with 5% saccharin and a 10% reduction could be achieved without saccharin supplementation. In case of without pre-baiting trial, a 10% reduction in burrow activity was recorded in case of saccharin supplemented groundnut – maize grain bait impregnated with strychnine alkaloid; while no reduction was recorded in case of no saccharin...

Masood ur Rahman1*, Zahid Mahmood2, Taj Ali2, Muhammad Aziz Irfan Mufti3, Jehangir Khan Seyal4, Munir Ahmad5

E-mail | masoodra@hotmail.com

 

Sarhad Journal of Agriculture, Vol. 31, Iss. 1
...ainst soil resistance in sandy loam soil bin. The gears were formed of commercial gun metal (Cu 87.76%, Sn 7.74, Zn 1.52%), gun metal (Cu 88%, Sn 7.38%, Zn 1.78%), and gear bronze (Cu 85.92%, Sn 4.96%, Zn 2.93%, Ni 1.23%). The gear compositions were determined using atomic absorption. The gears under test exhibited significant difference in surface wear among each other. As compared to gear bronze commercial gun metal and gun metal showed surface wear of 245% ...

Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*

 

...es in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distributions of both viroids also occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus host....

Rashid Saleem1*, Zammurad Iqbal Ahmad2, Muhammad Abbas Anees2, Abdul Razzaq3 and Ashiq Saleem1

 

...ld be required to attain same yield from respective sole cropping systems. Likewise, in fertility treatments total land equivalent ratio (1.78) was recorded in PK + inoculation treatment. It means that 78% more area would be required to achieve similar grain yield from control plots. Due to intercropping of legumes in maize and with application of poultry manure improved the organic matter and nutrients of soil.

 

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Malik Ikram Ullah1, Abdul Aziz Khakwani1*, Muhammad Sadiq1, Inayatullah Awan1, Muhammad Munir2, Ghazanfarullah1

...fodder maize cultivar Kissan. The experiment was conducted on silt loam soil at Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan during 2011. Six nitrogen treatments (80, 120, 160, 200, 240 and 280 kg ha-1) and a control one were studied in this trial. Growth and yield traits and quality such as plant height, stem diameter, leaf area plant-1, leaf area index, chlorophyll content, green fodder yield, dry matter yield, crude protein, crude fi...

Muhammad Mudassar Maqbool1, Anser Ali1, 2 *, Tanveer ul Haq1, Muhammad Nasir Majeed1, Dong Jin Lee2

 

... wheat varieties viz. Faisalabad-2008, Lasani-2008 and Kohistan-97 were manually sown in polythene lined pots. Five water stress treatments were created by withholding the irrigation for specified time at critical growth stages viz. tillering, booting, anthesis and milking (grain filling), whereas a well-watered treatment was also included during whole plant growth and development period. The results showed that water stress...

Nisar Ahmad1*, Inayatullah Jan2, Saif Ullah1 and Sidra Pervez1

 

... village. Thus the total sample size was 160 farmers. Cobb Douglas Production Function (CDPF) was used to analyse the data. The results of the study indicate that credit has positive effects on wheat productivity. Analysis shows that only 30% of credit users are utilizing loan for the purchase of seed and fertilizers while 70% are utilizing loan for other purposes as for marriages & ceremonial and for purchase of agriculture land & tractor etc. Similar...

Dallin D. Oaks

 

... have assumed to be necessary for the current diversification of languages.

 

...

Muhammad Rizwan Tariq1*, Muhammad Issa Khan1, Aysha Sameen1, Mahr un Nisa2

 

...ll fulfil omega 3 Poly unsaturated fatty acid (PUFA) concentration that contributes 8.5% of the total fatty acids. Rabbits were reared for the period of eight weeks. Enrichment of 7% flaxseed in the diet of rabbits significantly enhanced growth performance of rabbits and improved poly unsaturated fatty acids in the meat of rabbits as compared to other groups.

 

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Hayat Badshah1*, Farman Ullah1, Abid Farid2, Ahmad-U-Rahman Saljoqi1, Sajjad Ahmad3

...e Laboratory, it is necessary to know the development capacity of the cotton mealybug, Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae), on different host plants. Such studies would identify the best host plant for its efficient rearing. The present study investigated some basic life table parameters i.e. female life span, adult female weight, longevity and reproductive potential of P. solenopsis on okra (Abelmoschus esculentus Linn.), brinjal (...

Kemi Funmilayo Omotesho1*, Isreal Ogunlade1, Opeyemi Eyitayo Ayinde2

...using a two-stage random sampling technique. Data used for the study were analysed using descriptive statistics, Likert scale and the regression analysis. The study revealed that farmers perceived the level of accountability of extension to farmers in the study area as poor. Level of education, access to training and membership of farmer-groups negatively influenced accountability at p<0.05. Based on the findings of the study, it is recommended that agricul...

Asma Noshad1, Mudassar Iqbal1*, Zafar Iqbal1, Hamida Bibi2, Saifullah3, Salma Bibi4, Hamid Ullah Shah1

...rtality of Macrosiphum rosae (rose aphids) and the LC50 values were calculated and used as an indicative of insecticidal potential. The maximum mortality of aphids was achieved at 80 µg mL-1 and the LC50 for the fruit body extract after 24 HRS was calculated as 12.83μg mL-1, followed by the extract from the fermentation filtrate with LC50 value 25.03 μg mL-1. The mycelial extract wit...

Muhammad Shah Zaman1, Ghulam Muhammad Ali2, Aish Muhammad1, Khalid Farooq2, Iqbal Hussain1*

...te) was investigated for salt tolerance at 0.0, 10, 20, 40, 60, 80, 100 mM NaCl supplemented with MS medium. Kroda emerged as the most salt tolerant variety with the highest plant height (6.5 cm), number of nodes (8.8), fresh shoot weight plant-1 (0.166 g), number of roots (4.6) and root length (2.5 cm) at 60 mM NaCl followed by Sh-5. Desiree and Cardinal were moderately tolerant varieties to NaCl stress. The most sensitive...

Faheem Khan*, Muhammad Zafarullah Khan

...ts namely Mardan and Charsadda of Khyber Pakhtunkhwa Province were selected for this purpose. A multi-stage sampling method was used to draw sample of 336 sugarcane growers for the present study and an interview schedule was used to solicit responses that addressed the research objectives. The findings from the study showed that 80 percent of respondents were illiterate while the 20 perce...

Imdad Sohu1, Allah Wadhayo Gandahi2*, Ghulam Raza Bhutto2, Muhammad Salim Sarki2, Rabail Gandahi3

...is of the soil and plant samples were recorded. The application of organic sources of farmyard manure and poultry manure in combination with inorganic NPK fertilizers had shown positive effect on chickpea plant height, number of branches plant-1, number of pods plant-1 and seed index. The growth and yield of chickpea significantly increased with the collective application of chemical fertilizers and organic manures. It is concluded that the application of hal...

Shahid Iqbal Awan1*, Syed Dilnawaz Ahmad2, Muhammad Amjad Ali3, Muhammad Shahzad Ahmed1, Aurangzeb Rao1

...ing in the gene pool was satisfactory for initiating a breeding plan. Relative water content, cell membrane stability, osmotic adjustment and grain yield per plant were major contributors towards variability. Grain yield per plant was closely linked to relative water content, cell membrane stability and specific flag leaf area. Eight clusters were formed through cluster analysis, and greater genetic distance was detected among the members of cluster 6 and clus...

Jonathan Jong

...n, or specifies the necessary and sufficient conditions for counting as religious. Rather, there are causally and phenomenologically distinct phenomena—such as the belief in supernatural agents, participation in rituals, formation of non-kin groups, obedience to moral codes, and so forth—that variously co-occur in packages we intuitively label as particular religions. Furthermore, these distinct phenomena are als...

Ethan G. Quillen

... sort of common or universal definition. This latter issue is, as this paper will argue, the result of a theoretical abstraction, a scholarly attempt at theorizing a general interpretation. This is evinced not just by the way the term has been contrarily defined, but by a number of novel approaches, such as the creation and use of umbrella terms such as ‘non-religion,’ or the precarious notion of a division between ‘positive’ and/or &...

Joseph Langston1*, Joseph Hammer2, Ryan T. Cragun3

...by nonbelief not being a salient part of their identity. Notably, approximately one third of secular nonaffiliates indicated that they would join such groups if they were locally available. Neither maximum accommodation nor confrontation with religion was indicated by a majority of nonbelievers, though more respondents opted for accommodation (60%) than confrontation (25%). Most respondents indicated that their willingness to attack or ridicule religion was no...

Gareth Longden

...the members of the organisation then published. The current research builds on Campbell’s work and offers a detailed profile of the membership of the British Humanist Association at the start of the 21st century. The results of a survey conducted in 2014 of 1,097 humanists are presented and compared with Campbell’s original 1967 data and against data derived from the 2011 United Kingdom Census. Particular attention is given to the topics of sex, ag...

 David F. Bradley*, Julie J. Exline, Alex Uzdavines

...to a previously reported sample of religious believers, nonbelievers described a god that was less loving, more distant, and more cruel. Using correlation and regression, we found that personality factors (Big Five personality traits, adult attachment style, and socially desirable responding) were not strong predictors of the characteristics nonbelievers assign to hypothetical gods. Seeing a hypothetical god as more loving, less cruel, and less distant was ass...

Rabbi Paul Shrell-Fox

...ligious intuition, it is safe to assume that over one’s lifetime, intuition and attitudes towards a belief in God is subject to fluctuation. Dennett and LaScola found this to be true with priests and ministers. That study was the catalyst for the current study of rabbis’ shift in religious beliefs in general, and belief in God in particular. Approximately 25 rabbis voluntarily submitted vignettes concerning their shifts in religious belief. These v...

Ghulam Ali Bajwa and Muhammad Arshad Khan

...n and Fe were found in Husang and Karyansuban, respectively. A significant variation in uptake and retention of macro- and micro nutrients was found in the mulberry varieties after fertilizer application. The highest N was taken up by Kanmasi, while the highest P and K were taken up by Husang. Iron and Cu increased, and Zn and Mn decreased after week 3 of fertilizer application. Nitrogen was 1.5 to 2.6 times greater after we...

Ali Muhammad*, Muhammad Ayub

...treatment such as water, salt and lye solution which were coded as RW1, RW2 and RW3 respectively. During treatment process the bitter glucoside level such as oleourepean leached out from the fruits to the solvents by osmosis process which alters the composition of olive fruit. Phenolic compounds, flavonoids and anthocyanins which are the major components of olive fruits degrades significantly during four weeks of treatment process. Results showe...

Gultaj Hussain1, Abdur Rahman1*, Tariq Hussain2, Siraj Uddin1, Tariq Ali3

E-mail | abdur_rahman@aup.edu.pk

...ed. A total of 3024 meat samples were collected from cattle slaughtered at Peshawar, Pakistan. Samples were processed and analyzed for TVC of E. coli and S. typhymurium at 0, 48, 144 and 288hrs during 12 days of meat samples refrig- eration storage at 4±1 °C. The pH of meat samples were checked and recorded on similar intervals. During 12 days storage, significant reductions (i....

Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*

E-mail | shizixue@gmail.com

...iodic outbreaks take thousands of lives. With rapid globalisation and climatic shift, JEV has started to emerge in previously unaffected areas. Scientific evidences show that JEV would remain a global pathogen and might cause worldwide pandemics. Currently, more research is required to be applied to JEV pathogenesis and antivirus therapy. This review aims to reveal the exigency of developing a worldwide effort to acknowledge...

Rick Repetti

...hoices are lawfully necessary consequences of prior events, then they are unfree; Derk Pereboom’s two arguments for hard incompatibilism: the manipulation argument, which asserts that manipulated choices are unfree, determinism is functionally equivalent to manipulation, and thus determined choices are unfree; and the randomness argument, which asserts that we cannot claim authorship
over random neural events; and Galen Strawson&rsq...

Opeyemi Eyitayo Ayinde1*, Kayode Ayinde2, Tofunmi Emmanuel Ilori1, Raphael Olanrewaju Babatunde1

E-mail | opeyemi@unilorin.edu.ng

Reviewed by Dr William Patterson

...Coyne has much of use to say on the topic and many of his points are powerfully made. His case is weakened, however, by his incoherent treatment of epistemology. This problem is serious in that it lies at the heart of the matter and serves to undermine, at least to some extent, parts of Coyne’s main argument.

...

Oladipo Elijah Kolawole*, Oloke Julius Kola, Awoyelu Elukunbi Hilda, Olasunmibo Oluwatobi Gabriel, Ogunyale Tolulope Temitope

E-mail | koladipo2k3@ yahoo.co.uk

...s in Ogbomoso. The blood sample of consented two hundred and seventy five (275) subjects were obtained and their sera were screened for antibodies to HEV using Enzyme Linked Immunosorbent Assay. Samples were collected over a period of 4 months (September – December, 2014). The mean age and mean CD4+ count of the subjects were 40.14±0.47 years and 387.28±18.91 cells/mm3 respectively. Out of 275 subjects te...

Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *

...ewage contaminated water samples were collected from two sites in Lagos, Nigeria. Samples were concentrated and inoculated into MCF-7 and RD cell lines. Isolates from MCF-7 were passaged in RD and L20B cell lines while isolates from RD cell line were passaged in L20B cell line. Subsequently, all isolates were subjected to panenterovirus 5l-UTR, partial VP1 and EV-C-3Dpol/3’-UTR PCR a...

Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2

...t-align: justify;">Blood samples from a total of 359 sheep from 15 farms were analysed for the presence of antibodies against bluetongue virus (BTV) by commercial enzyme-linked immunosorbent assay (ELISA). Antibodies were detected in 290 sheep from fourteen different flocks, distributed in all analyzed regions (Central, Chorotega, Atlantic Huetar, North ...

Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2

E-mail | Tomas.Jinnerot@sva.se

.... bronchiseptica in samples from dogs with respiratory signs. A genome comparison program was used to select a suitable target DNA sequence. A PCR targeting the bfrZ gene was developed which showed no cross-reactivity in silico or when tested with a panel of bacterial isolates. The limit of detection was determined to 4x103 bacteria per mL of nasal swab sa...

Claudia Kohl*, Andreas Nitsche, Andreas Kurth

Email: kohlc@rki.de

...his approach to clinical samples we can identify the community of etiological pathogens, without any knowledge on the targets in advance. However, clinical specimens usually comprise an overwhelming amount of host nucleic acids, which by far exceeds the number of pathogen nucleic acids in the sample. Subsequently, it is necessary to either decrease the amount of host nucleic acids or incre...

Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman

E-mail | irfanmudasir@gmail.com

...s when applied at high dosages. Therefore, pesticide application at high concentrations can be considered as a kind of stress, similarly to other stresses such as salt, drought and low or high temperatures.

...

 Nagina Zeb, Muhammad Sajid, Abdul Mateen Khattak and Imtiaz Hussain

...nd flower quality of chrysanthemum (Dendranthema grandiflorum) was carried out at Ornamental Nursery, Department of Horticulture, Khyber Pakhtunkhwa Agricultural University, Peshawar during 2011. Suckers of chrysanthemums were planted during the first week of January and transplanted in 20 cm clay pots in July. The experiment was laid out in Randomized Complete Block Design with two factors replicated three times. Different ...

Muhammad Imran1*, Muhammad Mobashar2, Muhammad Irfan3, Shazia Hanif4, Sumaira Hanif5 and Muhammad Abubakar6

...g with maize silage as basal diet. Four dietary treatments having MOLM were 0.0, 0.71, 1.42 and 2.16 Kg DM/day used as replacement for CSC containing 2.16, 1.42, 0.71 and 0.0 Kg DM CSC/day. Overall the apparent digestibility coefficients of dry matter (DM) and organic matter (OM) were non-significant (P>0.05) whereas digestibility of NDF was significantly increased (P<0.05) in treatment MLM4. When compared among treatments, the value of crude protein (CP...

Rick Repetti

... justify;">Theodicies of satisficing – defenses of God’s goodness that justify creating minimally satisfactory beings/worlds – originate with Robert Merrihew Adams (1972, 1979). Adams (1972) argued that in creating imperfect beings God was graceful in giving the undeserved gift of life. There have been many objections to Adams’s argument; e.g., Jerome A. Weinstock (1975) objected that God still would ...

Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3

...ssiana (Bals.) was bio-assayed against three larval instars of Atteva sciodoxa under laboratory conditions of 27±2ºC, 75% RH and 12:12 h photoperiod. Instar 3rd responded with 100% mortality at 5x107 conidia ml-1, while same level of mortality was recorded in 5th instar at 1x108 conidia ml-1. Instar 5 also showed the lowest mortality (33.3%) at 1x106 conidia ml-1. Median effective concentration (EC50) ranged bet...

Kang-Seuk Choi

... justify;">Infectious bursal disease (IBD) virus-like particles (VLPs) comprising only viral capsid proteins mimic the naive configuration of authentic IBD virus particles. The VLPs show intrinsic immunogenicity and a high safety profile.  Thus, VLPs are considered one of the most promising approaches to vaccine development and are an alternative to inactivated IBD vaccines. In addition, VLP technology has many applicat...

Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4

... and new methods. Paired sample t-test was used for comparison between two methods and different conditions (eyes open and eyes closed). Traditional method for stability analysis showed no difference between two conditions in one minute standing (p ≥ 0.05). However, the new method confirmed that stability parameters significantly decreased in five minutes standing (p<0.001). Moreover, patients were unstable in standing during the fourth and fifth minute....

Fatemeh Kheiri

...des was evaluated by two sample t-test. The physiological cost index of amputees varied between 0.42 and 0.74 beats per minute. there was no signi cant di erence between the range of motion of ankle, knee, hip and pelvic rang of motions between both sides. The plantar exion moment in prosthesis and sound sides varied from 0.92-0.98 N/BW and 1.19-1.39 N/BW, respectively (p value of di er- ence <0.05). The energy consumption of amputees was high due to reduc...

Seyed Mohsen Mirbod

...ring walking. It carried same performance as that of the knee cage, and additionally is more cosmesis, ease in use and is economical.

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Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6

...itu proximity ligation assay (in situ PLA), a selective tool for studying virus-host protein-protein interactions. BDV P (phosphoprotein) and N (nucleoprotein) have previously been reported to interact with several host proteins, thereby interfering with various signaling pathways. In this study, we focused on some of these interactions (BDV P-HMGB1, BDV N/P-Cdc2). First, we used rat glioma cell cultures persistently infected with a laboratory strain of BDV (C...

Rehmat Ullah1*, Muhammad Zafarullah Khan1 and Kalim Ullah2

...For the present study, a sample of 306 respondents was selected based on Sekaran sampling technique while using descriptive research design. Well-prepared and pre-tested interview schedule was utilized for collection of data. Descriptive statistics i.e. frequency, percentages and rank order was calculated. Chi-square test was performed to find out association among demographic characteristics and MFSC registration duration a...

Abiodun D. Olabode

...hin the demarcated forty-sampled quadrats from both irrigated and non-irrigated farms. Information on socio-economic activities of the rice farmers, level of rice yield, agricultural practices for rice production and farm management techniques employed in the study area were observed. The study employed simple percentages to analyse the farmers’ responses; while regression model established significant relationships between rice yield and farm management...

John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1

...e microplate-screening assay (ELASA). Top three candidate aptamers were then paired in a matrix of all possible capture and reporter aptamer combinations and tested in a fluorescent peroxidase-linked Amplex® UltraRed (AUR; a resazurin-like substrate) version of a rapid (< 1 h) aptamer magnetic bead sandwich as

Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren

...in serum and dried blood samples collected from mink, a VP2 ELISA (enzyme-linked immunosorbent assay) system was recently evaluated. However, such estimations of disease progression are commonly based on single sampling occasions and it is essential to know if estimated antibody levels vary over...

Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3

... and out of that 40 feed samples 2 from each farm were collected in the month of April. All the samples were analysed for the determination of aflatoxin B1 by using reverse phase High Performance Liquid Chromatography (HPLC. Out of total samples Aflatoxin B1 was detected in 33 feed samples, with a percent contamination rate of 83%. The level of aflatoxin...

Shahida Bibi* and Ijaz Ahmad Khan

...ds pod-1, thousand grains weight, grain and biological yield of mungbean were also found significant. In main plots, weed density m-2 (16.47) and fresh weed biomass (529.8) were lower in herbicide treated plots in mungbean crop. Number of seeds pod-1 (10.85), thousand grains weight (30.15g), grain yield (366.56 kg ha-1) and biological yield of mungbean (1306.7 kg ha -1) ...

Nazish Huma Khan*, Mohammad Nafees and Adila Bashir

...ls (Cd, Cr and Ni). Soil samples were collected from both orchard and non-orchard fields and were extracted with Aqua-regia and Mehlich-3 extracting solutions for total and available metals respectively. Wheat crops from both fields (orchard and non-orchard) were collected and analyzed with aqua-regia for heavy metals contents. Results showed that the average concentrations of metals in soils of orchard and non-orchard fields were as chromium (Cr) 56.9 and 52....

Mukhtarullah1, Jawad Ali2 and Mohammad Akmal1*

...>, spike weight (g), thousand grains weight (g) which affected the grain yield. Based on biomass, which contributes from plant height and grains weight, varieties Lalma, Chakwal-50 and Pak-2013 could be the best varieties for planting early to late in season in the area. Delay sowing of wheat crop due to dry weather i.e. late rainfall and/or eventuality of the previous crop of the cropping system could result higher wheat production from the variety Pak-2013 i...

Nadeem Khan1*, Arshad Ali Khan1, Mukhtar Ahmad2, Muhammad Nouman2 and Badshah Islam1

Ahmed Zein Mahmoud1, Muaz Abdellatif 2, 3*, Luai Shazali1

...lah, Saudi Arabia. Serum samples (n=400), collected during 2012–2013 from sick and clinically healthy herds, were subjected to antibodies detection using c-ELISA. Out of examined animals, 83 (62.9%) goats and 70 (33.2%) sheep were detected positive against PPRV antibodies, whereas camels appeared to be seronegative. Based upon the seasonal variations in the antibodies detection, envi...

Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*

 

...etected from post mortem samples (lymph node, spleen and trachea) of goats and a sheep during the year 2008-2012. The partial N gene of ten isolates and partial F gene of eight isolates were amplified by RT-PCR, and were sequenced. The phylogenetic analysis revealed that PPRVs circulating in Bangladesh belonged to Lineage IV and they formed a separate sub-cluster along with recent isolates from Nepal, Bhutan and China. On genetic analyses, it appeared that the...

Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4

...signs of PPR including nasal and ocular discharges, high temperature, diarrhea and ulcerative lesions in the oral cavity. A total of eighteen goats from a herd of sixty, were affected and two goats succumbed within two weeks. Interestingly, the disease was exclusively observed in goats and all sheep kept in the same herd were serologically positive but did not show any clinical signs of PPR. The active PPR virus (PPRV) infec...

Muhammad Jurial Baloch1*, Ghulam Murtaza Channa2, Wajid Ali Jatoi2, Abdul Wahid Baloch1, Imdad Hussain Rind1, Muhammad Ahmed Arain1 and Ayaz Ali Keerio1

... TJ-83 × Sarsabz recorded with higher estimates for grains spike-1, grain yield plant-1, seed index and harvest index. Thus both the F1 hybrids may be choice breeding material for hybrid crop development to improve the yield traits.  

...

Muhammad Siddiq1* and Hamid Ullah Shah2

 

...ed in sole palm oil. The samples kept in sunlight showed maximum PV (12.82%), FFA (1.32%) and AV (1.39), while maximum IV (63.10), CI (0.371) and BC (11.30) value were observed in samples stored at ambient temperature. Storage conditions increased PV from 218 to 27.82, FFA from 0.88 to 1.47, AV from 1.02 to 1.56, while decreased IV from 63.38 to 58.05, CI from 0.392 to 0.337 and BC from 27.87 to 3.46. It was concluded that o...

 Aqila Shaheen

...on eight soil series (Missa, Pirsabak, Guliana, Rajar, Balkassar, Rawalpindi, Chakwal and Satwal) in tehsil Gujar Khan of Rawalpindi district. Amongst the soil characteristics of selected fields, pH ranged from 7.36-7.94 for surface soil (0-15 cm) and 7.49-7.94 for subsoil (30-45 cm), EC1:1 was 0.22-0.38 dSm-1  in surface soil and 0.21-0.28 dSm-1    in subsoil, CaCO3 was 1.0...

 Farmanullah Khan, Samad Khan, Wiqar Ahmad and Imran Khan

... density, increased WHC, saturation percentage, organic matter content, plant available N, P and K significantly (p<0.05) both at 0-15 and 15-30 cm depths. Effect of fertilizer sources either alone or in integrated form was non-significant on Cu, Zn, Fe and Mn concentration. Economic analysis showed that NPK75FYM20 rewarded the maximum net income of Rs. 43108 ha-1, indicating its superiority for profitable wheat yield under rain-fed conditions. It can be co...

 Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid

 
...Pb, Cd and Cr in albumen samples collected from Peshawar were 0.13, 0.06 and 0.09 (ppm) respectively. Concentrations of Pb, Cd and Cr in egg albumen of Dir Lower were 0.13, 0.05 and 0.05and in Malakand were 0.12, 0.05 and 0.07 ppm, respectively. The mean concentrations of essential elements Fe, Mn and Zn in egg yolk of Peshawar were 1.27, 0.31 and 2.05 while that of Dir Lower were 1.05, 0.19 and 1.97±0.04 ppm, respectively. The mean levels of Fe, Mn and...

 Inamullah Khan, Muhammad Subhan Qureshi, Rajwali Khan, Syed Muhammad Sohail, Ijaz Ahmad, Muhammad Shoaib and Asim Ijaz

...tal of 410 and 202 blood samples were collected from cattle and humans respectively. Total 40 samples of subsistence farms and 30 nomadic herds were analysed. Forty eight farm workers, 40 farm owner 36 abattoirs workers, 15 veterinary assistants, 15 veterinarian and 40 butchers were also screen out. Overall sero-prevalence in cattle was 15.36% on SPAT, 14.39% by i-ELISA

 Gul Naz and Mohammad Akmal

...e. Saleem-2000, Uqab, Pirsabak-2005, Tatara-96 and Pirsabak-2004 as subplot treatment. Results revealed higher spikes m-2 (286), grains spike-1 (38.55) and thousand grains weight (44.67 g) which resulted maximum grain yield (3308 kg ha-1) from N-rate 150 kg ha-1 as compared to the control. Among the varieties for yield and yield traits, Pirsabak-2005 pro...

 Umed Ali Laghari, Ahmed Naqi Shah, Muhammad Nawaz Kandhro, Zia-ul-Hassan, Ghulam Murtaza Jamro and Khalid Hussain Talpur

...ally grass pea (Lathyrus sativus L.). We evaluated K requirements of five elite grass pea genotypes in a field experiment. The experiment was organized in factorial block design with three replications. The study involved five commonly grown grass pea genotypes (Sel-B 111, Sel-449, Sel-190, Sel-1785 and Sel-945) and three K doses (0, 10 and 20 kg K ha-1). The results revealed that K nutrition and genotypic variation significantly (p<0.05) determined the yie...

 Arif Khan, Abdur Rahman, Shoail Akhtar, Siraj Uddin, Zahid Ullah and Nazir Ahmad

 
...e hundred and eight beef samples from Longissimus dorsi muscle were collected from three age groups (i.e. group 1= 12-18 monts, 2 =18.1-36.0 months and 3= > 36 months) of Kundi and Nili Ravi breeds of buffalo. Samples were obtained from freshly preselected slaughtered buffaloes at local municipal abattoir Peshawar. The effect of breed and animal age on marbling, water holding capacity (WHC) and tenderness and sarcomere le...

Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5

...ochrome b (CytB). Twelve samples of laboratory-reared population of each species were used for the study. Sequencing data were used to calculate haplotype, haplotype diversity and nucleotide diversity for the two species. Based on combined loci of cytochrome oxidase II (COII) and cytochrome b (CytB), twelve haplotypes were generated for G. p. palpalis and five for G. m. submorsitans. Similarly, for the combined loci, haplotype diversity (Hd) was ...

Jannatul Noor, Md. Ahaduzzaman, Mir Md. Afzal Hossain, Mohammad Alamgir Hossain, Sardar Abdur Rahim and Md. Samun Sarker

...es using random positive sampling technique. Among the breed, Black Bengal goats were highly affected (53.33%) followed by Jamuna pari (33.33%) and cross breed (13.33%) (P=0.00). Black coat coloured (53.33%) were mostly infested than another coat coloured animals (P=0.00). Age less than 6 months goats were mostly affected (55.0%) than adult goats (26.67%) (P=0.00). In this study, males were more prone (58.33%) than female goats (41.67%) (P=0.036). The ticks we...

 Muhammad Nawaz Kandhro, Qamaruddin Jogi, Mahmooda Buriro, Aijaz Ahmed Soomro, Ghulam Mustafa Laghari and Ali Nawaz Khaskheli

 

...ory effect. In both aforesaid treatments, lowest and statistically similar values of germination, root/ shoot length and fresh/ dry weight seedling-1 were recorded for both C. arvensis and C. rotundus, respectively. The water extract of E. camaldulensis showed superiority in allelopathic effects over its powder. Inhibitory effect of water extract or powder increased at higher rate (30 ml or 30 g kg-1 soil). Hence, results conferred that eucalyptus leaves posse...

 Ian von Hegner

... generalized to be universally applicable, then the concept of hypothetical gods’ right to rule results in dictatorship. Whereas the concepts of dictator and tyrant originally had a more positive meaning, those meanings have changed. However, the concept of the gods in the philosophical debate has avoided a similar redefinition in light of democracy, despite the fact, that it involves the same negation of modern fundam...

Yingbin Wang*, Ting Ye, Xiaogang Wang and Congyu Zhou

...h since they present the same trend, but it is not as critical as imagined. Therefore, the variation of the catch of P. trituberculatus in the northern East China Sea was synthetically affected by multiple factors. More studies should be carried out to explore other potential factors that may affect the catch, and find out the underlying mechanisms.

...

Amtul Jamil Sami1*, Madeeha Khalid1, Sara Iqbal1, Maira Afzal1 and A.R. Shakoori2

...xt-align: justify;">Chitosan is a cationic biopolymer that can be used for sustainable waste water treatment as a coagulant-flocculent due to its ability to coagulate various ionic substances such as metal ions as well as dyes. In the current study novel polymer composites were prepared using natural polymers chitosan and starch in molds. Structure of composites was analyzed using scanning electron microscopy. The physical p...

Mian Shamas Murtaza1, Aysha Sameen1, Nuzhat Huma1 and Fatma Hussain2

...e Cheddar cheese. Cheese samples were manufactured by adding xanthan gum and guar gums individually @ 1.5, 3.0 and 4.5% in low fat cheese (2% fat) besides the negative (2% fat) and positive (4% fat) control samples. The cheese samples were ripened at 6-8oC for 30 days and analyzed for physico-chemical composition, functional, textural and sensory quality. The results showed that reduction ...

Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek

...lated from root and soil samples collected in Prague, Czech Republic, were cultured on agar media and tested against Meloidogyne hapla both in-vitro and in-vivo. All fungi proved to be efficient in reducing final population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) ...

Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek

...l nematicides, Vydate, Basamid (G) and fertilizer, Calcium cyanamide through soil application to control sugar beet cyst nematodes Heterodera schachtii. Lecanicillium muscarium decreased nematode population significantly (P=0.05 and caused reductions in final numbers of cysts per 250 g of soil, eggs and juveniles per cyst. Nematicides effects were not quite significant among each other but had tremendous effects in reducing nematode population compared to that...

Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1

Usman Ijaz1, Muhammad Sajid Tahir2, Khalid Abdul Majeed3*, Shahid Iqbal4, Iffat Huma5, S. Firyal6, Ijaz Ahmed7, Shahid Chohan8 and Aamir Riaz Khan9 

...bunit I (COI) gene. Scat samples of 15 different known leopards were collected from Nathiagali, Khyber Pakhtunkhwa, Pakistan and Azad Jammu and Kashmir, Pakistan. After amplification with specific oligos, the amplicons of partial region of COI were subjected to sequencing, and then observed for single nucleotide polymorphisms (SNPs). SNPs were observed on three loci of the COI gene as compared to reference sequence of Panthera pardus pardus. The specime...

Sungil Kim1, Muhammad Riaz2, Amjad Farooq3 and Sungkwon Park4*

... beneficial effects on unsaturated fatty acid formation in pork without deleterious effect on growth performance.

...

Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

...d and sixty six ear swab samples (n=166) were cultured from which eight (8) Mycoplasma species were isolated and characterized using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium chloride and hydrolysed casein. They had no phosphatase activity, did not produce ‘film and spots’ and neither hydrolysed arginine nor urea and were preliminarily identified as...

Muhammad Aslam

...on-nexus, reclamation of salinized lands, improved agricultural credit and support price policies. Recommendations include improving agricultural research and extension systems, accelerating diffusion and adoption of latest agriculture technologies and inputs, enhancing good quality seed production, improving irrigation water management and improving reclamation and drainage.

...

Gerald E. Marsh

... have no meaning. This essay explores the epistemological basis of this lament.

...

Ghani Akbar, Muhammad Munir Ahmad, Muhammad Asif, Iqbal Hassan, Qurban Hussain3 and Greg Hamilton

...erbate water logging and salinity and reduce the water productivity of major crops. Addressing these issues, a long term experiment (2000 to 2009) under wheat-maize (9 seasons each) cropping pattern was conducted on raised beds (with furrow spacing of 65 cm (Narrow Bed-NB), 130 cm (Medium Bed- MB) and 180 cm (Wide Bed-WB)), with controlled traffic regime emplaced, in comparison with traditional intensive cultivated flat basin (FB) for evaluating impacts on soi...

Haidar Ali, Malik Muhammad Shafi and Himayatullah Khan

...of Khyber Pakhtunkhwa. A sample of 201 farm households (Owners, Owner-cum-tenants and tenants) was selected using random sampling technique and data were collected through a pre-tested interview schedule. This study is focused on two selected districts of Peshawar and Charsadda. Four villages were studied two each from the sampled districts. Analysis sho...

Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2

...nfectious agents is necessary. Identification of the genes, and their variants, involved in innate immune responses is essential for the understanding of this inflammatory disease and to identify potential genetic markers for resistance to mastitis. This article presents a systematic integration of complex biological interactions of 226 mammary gland genes to uncover underlying regulatory networks. Data were collected from various databases after having an exh...

Aqila Shaheen and Mehwish Matien

...Mollisols (site 2). Soil samples were analyzed for bulk density (BD), soil organic carbon (SOC), total nitrogen (TN), available phosphorus (AP), extractable potassium (K) and pH. Statistically (p ≤ 0.05) higher SOC contents of Inceptisols and Mollisols were 14.18 g kg-1 and 23.42 g kg-1, respectively under forest land use. At both sites forest soils had higher SOC and AP while K was higher in grassland use. Forest soils had slightly...

Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*

..., arrangement and codon usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii,

Haji Muhammad, Zafar Iqbal* and Saira Saleemi

...na of Indus River at Taunsa Barrage, situated in South Punjab Pakistan. The survey was conducted from September 2013 to August, 2014 at eight sampling sites to ascertain fish diversity and their distribution pattern. A total 2249 fish specimen were collected using a variety of fish nets and identifications of these fishes were made by using standard taxanomic keys on the basis of morphometric characters. Seventy species belo...

Irfan Ullah and Abbas Ullah Jan

...stant. Imposition of any sale tax could create huge loss in consumption for these commodities. The demand for most of food groups are own price inelastic in both regions except milk, rice, tea and coffee and backed products which are more than unit elastic to own price. Similarly the demand for most of food groups in both regions are less than unit elastic to total expenditures and have positive sign, while the demand for milk, meat, rice, other cereals and ot...

Zaheer Ahmad Qureshi and Khuram Nawaz Sadozai

...and expenditure level of sampled House Hold. The HIES data set of year 2011-12 for KP Province was comprised of the total of 1352 sampled House Hold. However, the sampled rural House Hold was 926. The statistical framework comprised of Lorenz curve and Gini Coefficient to gauge the income inequality in the study area. To assess the income inequality both income and expenditure approaches a...

Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2

...e used and leaf dip bioassay was conducted. Pesticides were tested for their compatibility with N. barkeri at different concentrations under controlled laboratory conditions. The results showed 25%, 30%, 70%, 50% and 65% mortality occurred by tested pesticides at field relevant doses after 144 h respectively. Missing mites data indicated repellency due to pesticides, was highest at the start of experiment, and then these acclimatized on leaf discs. Thiacloprid...

Ayanda Opeyemi Isaac,1,* Oniye Sonnie Joshua2 and Auta Jehu2

 

...y used herbicides, glyphosate and paraquat was evaluated through changes in fish behaviour and mortality. Juveniles of the African catfish were exposed to varying acute concentrations of glyphosate and paraquat. The fishes responded, exhibiting different behavioural abnormalities like hyperactivity, abnormal swimming, restlessness, loss of equilibrum and haemorrhage. Observation of opercular ventilation count (OVC), tail fin...

Ambreena Hafiz1, Tanzeela Riaz2 and Farah Rauf Shakoori1*

...and 25%) also showed the same trend of elevation in the 4th and 6th instar larvae and adult beetles, respectively compared to susceptible population. Glucose contents were significantly enhanced in Gujranwala (25, 336 and 356%), Okara (36, 163 and 74%) and D.G khan populations (122, 250 and 311%) for 4th and 6th instar larvae and adult beetles, respectively. Glycogen level, on the other hand, was significantly reduced in Gujranwala (82, 74 and 83%), Okara (27,...

Syeda Farzana Bibi*, Lal Badshah and Siraj-ud-Din

...-1) from Peshawar to Charsadda Interchange. A total of 30 different species ,which belong to 29 genera and 16 families, were recorded from the area.Poaceae was the most dominating family including 7 genera. Ecological characteristics like life form spectra indicated that Therophytes (53.33%) were the most dominating life form. Leaf size spectra showed that the most abundant leaf size was Microphyll (50%). This study not only gives information about the flora o...

Muhammad Basit1, Shafqat Saeed2, Mushtaq Ahmad Saleem3, Rana Zulfiqar4

 

...009. Spodoptera spp, Thysanoplusia orichalcea and Heliothus were also found but in small numbers. Among natural enemies, ladybeetles and spiders were found to be the putative predators with maximum population of 3.9 and 3.5 individuals/plant, respectively. Further, correlation study indicated that syrphids and chrysoperla had a significant positive correlation with A. gossypii and T. orichalcea, respectively. Likewise, spiders and lady beetles had significan...

Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek

...nematicides Vydate and Basamid (G) with the fertilizer calcium cyanamide in the control of root knot nematode, Meloidogyne hapla, through soil application under greenhouse conditions. A significant (P=0.05) reduction in the number of galls and egg masses, eggs and juveniles (J2), and the reproduction factor (Rf=Pf/Pi) was observed in the plants treated with L. muscarium compared to the control. However, all chemical nematicides were also effective in reducing ...

Afzaal Ahmad Naseem1*, Maleeha Akram1, Sarwat Jahan2, Kiran Afshan2, Zubaria Iqbal1, Faheem Tahir3, Mazhar Qayyum1 and Syed Shakeel Raza Rizvi1

 Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5

...nship between nematode dosage and production of infective juveniles (IJ) of Steinernema feltiae and ability to locate hosts, therefore, in the present studies investigations were done on these aspects. A significantly greater emergence of IJ of S. feltiae from Galleria mellonella was observed with White traps than with modified Baermann trays. Maximum juveniles of S. feltiae emerged at 50 IJ dose followed by 100 IJ. The emergence of IJ decreased significantly ...

Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A

... (CIA) and infectious bursal disease (IBD) and co-infection of CIA and IBD. Infectious bursal disease was tentatively diagnosed in 5,000, 5 weeks old Hy-Line and ISA Brown pullets in a commercial poultry farm in Jos, Plateau State, Nigeria with mortality of up to 79.12%. The clinical signs observed were prostration, ruffled feathers, diarrhea, anorexia, ...

John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure

...d animal blood and urine samples will be collected at the household level whereas soil and water samples will be collected at the community level. Attempts will be made to isolate Leptospira bacteria from urine, soil and water samples. Antibody detection in animal and human serum samples will utilize Microscopic Agglutination Test (MAT). Study outcomes w...

Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2 

...extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production performances including age at first egg (AFE), laying rate (LR), egg production (EP), egg weight (EW), average egg weight (AEW). Two polymorphisms (G50831C and G62976A) were detected and located in exon 42 and intron 54 in HTT gene, respectively. One of them (g. 50831 G>C) was a missense mutation. Genotype and allele frequency analysi...

Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2

... determined using DPPH assay and MTT method, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The results of the phytochemical analysis showed that the extract has high polyphenolic content while has relative low flavonoids contents. In the antiradical scavenging property test the extract showed ...

Shakeel Ahmed* and Sarwat Jahan

...ted intravenously. Blood samples were collected individually 60 min before and 120 min after NMS administration at 15 min intervals. The plasma adipokines concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly increased plasma adiponectin (P<0.001), while decreased leptin (P<0.001) and resistin (P<0.01) concentrations compared to normal fed monkeys. No significant ...

Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3

..., the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method. This study was conducted to determine three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed. In TGC samples, C allele frequencies related to UoG-CAST, CAPN1 316 and CAPN1 4751 polymo...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

... Four fungal (Candida, Fusarium, Penicillium and Saccharomyces) and two bacterial genera (Klebsiella and Nocardia) were isolated from bolus only, three fungal (Cryptococcus, Histoplasma and Trichophoton) and six bacterial (Acaligenes, Azotobacter, Bartonella, Nitrsomonas, Pseudomonas and Salmonella) genera were isolated from guano while five fungal (Alternaria, Aspergillus, Chrysosporium, Exophilaand Scopulariopsis) and four bacterial (Bacillus, Corynebacteriu...

Muhammad Sohail1,*, Muhammad Naeem Khan1, Naureen Aziz Qureshi2 and Abdul Shakoor Chaudhry3

...he DNA damage by comet assay. The tail DNA (%), comet tail length and olive tail moment (OTM) were the parameters selected to detect DNA damage. Low doses (120 µg L-1) of each metal induced significantly higher levels of DNA strands breaks as compared to medium dose (240 µg L-1) and very low levels of DNA damaged was observed at high dose (360 µg L-1). Cu and Pb showed significantly higher value of % of tail DNA (56.74±1.81, 47.36&plus...

Jose M. Rojas, Noemi Sevilla and Veronica Martin

...s virus (PPRV) is the causal agent of a highly contagious disease that affects domestic and wild small ruminants, Peste des petits ruminants (PPR). Due to the important economical losses caused by this virus in Africa and Asia, the development of more effective, targeted and successful treatments for the disease are necessary. Like other Morbilliviruses, PPRV induces a suppression of the immune system during the acute diseas...

Abdur Rahim1, Ghulam Abbas1, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Noor Khan4, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6

...re Abdul Baqi, Ghulam Hussain and Kampa Industry (Unit 2) which produced 300 to 500 metric tons per month. Proximate composition of fish meal samples collected from different units showed that protein contents were 50.51% – 61.26% and energy was determined as 4042.0 cal/g – 4558.0 cal/g. Dry mater was calculated as 87.43% – 93.13% and fat was noted as 15.29% – 26.23%. Ash was found to be 12.32%–...

Sabri Unal1, ASır Er2, Erol Akkuzu1* and Lubomir Salek3

...fficacy on Macrosiphum rosae (Linnaeus) (Hemiptera: Aphididae) in Kastamonu, Turkey. Rearing of C. septempunctata was carried out at 21.3±4.00ºC and 78.8±9.44% relative humidity. The first, second, third and fourth larval instars of C. septempunctata consumed 20.3, 54.3, 108.2, and 232.7 M. rosea, respectively. The predation efficacy of the C. septempunctata was increased with the progress of the larval instars up to the fourth which was the...

Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*

...ulocytes count in plasma samples of the live P. chabaudi infected group in comparison to either the control or the gamma irradiated P. chabaudi infected group. Additionally, live P. chabaudi infection has resulted in histopathological changes and increased apoptotic cell death in splenic tissue of infected mice. Moreover, infection with live P. chabaudi was accompanied with an increase in NO, H2O2, and MDA levels in splenic tissue of lupus mice in comparison t...

Dallin D. Oaks and Cynthia L. Hallen

...ey are incorporated into sacred texts. This would seem to be the case not only for Judaism, Islam, and mainline Christianity, but also for Mormonism, a distinctive branch of Christianity. Officially known as The Church of Jesus Christ of Latter-day Saints (LDS), Mormonism shares some root metaphors with other Christians but differs in important ways in its development and use of these metaphors. In this paper we discuss the journey, battle, and container metap...

Imed Ben Salem*, Aymen Ben Ibrahim, M’barek Chetoui and Said Nouira

...mulus, L. macculus, L. rosalia, L. striatus and L. zebra; which have practically similar external morphologies although they spread in completely different geographical areas. The approach adopted was to identify landmarks on the skulls of all specimens using the tpsDig software, and then analyzing them through the program MorphoJ. Our results show that L. griselda has the largest skull, whereas L. zebra has the smallest. L. griselda and L. ro

Faiqah Ramzan*1 and Muhammad Haris Ramzan2

...itoneally in different dosage concentrations (1μg, 1 ng, and 10 ρg) to adult male mice twice daily for 12 days. Prostatic citric acid levels were determined photometrically. The levels decreased significantly at all tested doses. Intermittent administration of Kisspeptin –10 negatively regulates the prostatic citric acid levels in adult male mice.

...

Abdul Sattar Baloch1*, Abdul Rasheed1,2, Rahmatullah Rind1, Jam Kashif Sahito1, Rehana Buriro1, Muhammad Faisal Ayoob1 and Parkash Dewani1

... the testing of 100 sera samples and 50 milk samples from camels of three districts of Sindh province of Pakistan. Rose Bengal Plate Test (RBPT), serum agglutination test (SAT) and competitive-ELISA (c-ELISA) wer...

Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2

...ommercial layers from Faisalabad district of Punjab, Pakistan. Overall 120 blood samples, 24 tissue samples from each organ (trachea, lung and intestine) were collected from the 12 commercial layer flocks selected randomly at the age of 35-50 weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 birds was tested for antibodies against H9N2 by using Ha...

Rashid Ahmed Khan* and Muhammed Naveed

...tive and environmentally safe alternative to the other conventional insecticides used for the management of B. zonata.

...

Abdul Mannan Babar1* and Abdul Hannan Nagi2

...drium, cartilage, and dorsal perichondrium punch excision, leaving behind only dorsal skin.

...

Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*

...China. A total 454 serum samples were collected from Tibetan pigs in Nyingchi in 2014 and 2015. Each sample was assayed for T. gondii antibodies by a commercial enzyme-linked immunosorbent assay kits and an indirect hemagglutination test. The results showed that seroprevalence in Tibetan pigs were 25.6% by ELI

Atta ur Rehman1, Sher Ali2 and Sajid Malik2*

...t affinities between the samples obtained from Lakki Marwat and Tank districts, whereas the least affinities were evident between Karak and South Waziristan Agency populations. Collectively, these results show the presence of sub-populations in lower KPK which may suggest a disturbed nature of these populations. Further studies are needed to understand the nature of differentiation between these Pashtun populations.

...

Hussain Ahmad1, Inamullah1, Ijaz Ali2, Tauseef Ahmad3, Muhammad Tufail4, Kabir Ahmad5 and Bibi Nazia Murtaza3,6*

...uded in the study. Blood samples were collected and brucellosis was detected by Standard Plate Agglutination Test (SPAT) and Serum Tube Agglutination Test (STAT) and later on confirmed by PCR. Disease was detected in 3.66% (11/300) individuals by SPAT and 2% (6/300) by STAT and confirmed in 2.66 % (8/300) by PCR. The prevalence of disease in females was found to be higher as compared to males (in current studied group). The age group 31-40 years, was found to...

John G. Bruno

...gainst extracted lipopolysaccharides (LPS) of E. coli O157 and the so called “Big six” non-O157 Shiga-toxin producing E. coli (STEC) serovars (O26, O45, O103, O111, O121 and O145). These aptamers were assessed by enzyme-linked aptamer sorbent assay (ELASA) to rank their relative affinities and discriminatory ability for distinguishing between...

Muhammad Shafiq and Tahir Maqsood

...rsity of Agriculture, Faisalabad, during 2013-14. Adsorption procedure was done by harmonizing 2.5g soil in 0.01M CaCl2 solution having diverse B doses (0, 0.01, 0.03 , 0.05 , 0.07, 0.09, 0.11, 0.13,0.15 and 0.17 mg B L-1). Freundlich and Langmuir adsorption models were applied to the data to check the sorption of B on sandy clay loam soil. Freundlich model depicted superior fitness of the sorption (r2=0.99) statistics than ...

Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi

...bjected to serological assay for IgM using enzyme-linked immunosorbent assay. The mean age of subjects was 39.5±0.48 years and the mean CD4+ was 384.27 ±17.77 cells/µl. Out of 278 subjects tested, 11 (4%) were positive for anti-EBV IgM. Anti-EBV IgM was highest in the age group 41-50 years, 5 (5.81%); females, 8 (4.85%); students (14.21%); and CD4+ 350-500 cells/µl, 4 (36.36%). The multivariate anal...

Dilawar Hussain* and Abdul Mateen

...trations have almost the same effect on the growth parameters tested except in the case of PER. T3 (0.5% 4TX+2 ppm AFB1) showed better PER (31.42±1.74) when compared to T4 (0.5% 4TX+ 4 ppm AFB1) group (27.76±0.67). Interaction between different growth parameters of tilapia fed AFB1 and 4TX supplemented diets showed that net weight gain (NWG) was significantly (p<0.01) correlated with average daily gain (ADG), specific growth rate (SGR), feed i...

G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2

...n gill and liver tissues sampled showed concentration and time dependent significant increase (p < 0.005) in the values of malondialdehyde, catalase, superoxide dismutase, reduced glutathione, alkaline phosphatase (ALP), alanine aminotransferase (AST) and aspartate aminotransferase (AST). Similarly, aluminium phosphide enhanced levels of AST, ALT and ALP as both concentration and time dependent in elevation were recorded when compared to the control. The us...

Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2

...was detected in only two samples (16%) (1.2 and 1.3ng/g), aflatoxin G1 and G2 were not found. Ochratoxin A was found more frequently in fresh (54.16%) than in ensiled (20.86 %) samples with a mean of 8.06 and 4ng/g respectively. Average values for total aflatoxins (TAFs) and OTA were below the permissible limits as per regulation of European commission (EU), i.e. 20ng/g and 10ng/g, respectively.

...

Abdul Aziz Khan1,*, Waseem Ahmad Khan2, Waseem Ahmad Khan3 and Najam-ul-Huda Khan4

Asmaa Hamid1,* and Samia Kalsoom2

...esterolemia were fed the same diet as the diabetic rats and were monitored for total serum cholesterol. It was found that both Bengal gram and chickpea had a significant effect on lowering glucose level and total cholesterol of these experimental animals. Bengal gram however, proved to have a significantly (p<0.05) greater effect on lowering of these parameters.

...

Abdul Baset1, Qun Liu1,*, Muhammad Tariq Hanif1, 2, Baochao Liao1, Aamir Mahmood Memon1,3 and Muhammad Mohsin1

...ield (MSY) of Indian oil sardine, Sardinella longiceps (Valenciennes, 1847, Family: Clupeidae), fishery resources from Pakistani waters. The catch and effort data (1994 – 2009) were analyzed by CEDA (catch and effort data analysis) consists of Fox, Schaefer and Pella Tomlinson models and ASPIC (a surplus production model incorporating covariates) contain Fox and Logistic models. The average annual landings of S. longiceps stocks were 38 353 t while the h...

Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...lated from five raw milk samples. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. Their sensitivity against three phages indicated broad host range of phages SANF and SA2, and relativel...

Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*

...ries from hepatopancreas samples in E. sinensis. After removing low-quality reads, short reads and reads belonging to mitochondria, a total of 278,260,960 clean reads corresponding to mRNAs were obtained, these reads covered a total of 34,780,598,273 bases. A total of 48,657 SSR loci were recognized, of which 10,310 unigene sequences contain more than one SSR. These genetic markers are ideal molecular markers for the following investigating genetic diversity, ...

Oluwasogo David Olorunfemi, Oluwasegun Adetokunbo Adekunle, Felix Olayinka Oladipo, Temitope Oluwaseun Oladele and Oladimeji Idowu Oladele

...lected using a two-stage sampling technique to select one hundred and sixty respondents for the study. A purposive selection of two Local Government Areas each from the two Agricultural Development Programme administrative zones (Zones C and D) in Kwara State where fish farming is prominent and well-practiced was carried out. Forty fish farmers were then randomly selected from the fish farmers’ association chapter present in each selected Local Governmen...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...tion strategy. The serum samples were analyzed for determination of metals and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequencies were not deviant from HWE in RA group. The results of present study indicates that Pb and Cr concentration differ ...

Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1

...s. Agar well diffusion assay was used to monitor the antimicrobial activity of these strains both in mono culture and mixed culture. Streptomycin sulphate (10 μgml-1) and Amphotericin B (5 mgml-1) were used as positive controls. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorganisms, wh...

Sidra-Tul-Muntaha1, Muhammad Sagheer1*, Mansoor-ul-Hasan1 and Shahbaz Talib Sahi2

...cted during 2013 from Faisalabad, Multan and Nankana districts of Punjab, Pakistan. Three concentrations of each plant extract (5, 10, 15 and 20%) and synthetic pyrethroids (0.01, 0.02 and 0.03%) were evaluated in this study. We observed significant results with each treatment. For both repellent and growth inhibitory effects, A. indica and deltamethrin were most efficient among plant extracts and pyrethroids respectively. At highest dose rates, more than 90% ...

Muhammad Saeed* and Iftikhar Hussain Khalil

...04), Kohat-10 (KT10), Pirsabak-08 (PS08) and Janbaz (JZ) were crossed in line × tester mating to develop 24 F1 hybrids. The resultant hybrids along with parents were evaluated during cropping season 2012-13 at Cereal Crops Research Institute (CCRI), Pirsabak, Nowshera, Pakistan under rainfed environment, using RCB design with 3 replications. General combining ability (GCA), specific combining ability (SCA) and narrow s...

Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar

...an. A total of 965 serum samples were collected from sheep and goat’s farms from January to December 2015 with no history of vaccination. Haemagglutination inhibition (HI) test was performed using PPRV antigen to determine antibody titres level. The overall prevalence of antibodies to PPRV in goats and sheep were 66.22% (521 samples) and 65.76% (444 samples), respectively. Sero-preva...

Neelum Andaleeb and Munir Khan

...study area. A multistage sampling method was used to select a sample of 274 livestock farm households from 6 union councils, and a well-structured questionnaire was used to collect data from the female respondents through face to face interview. Cobb-Douglas production function and cost benefit analysis were used to arrive at the main conclusion. Results show that feed cost was the major component of total cost followed by l...

Qamar Ali, Muhammad Ashfaq and Muhammad Tariq Iqbal Khan

...s by using simple random sampling. Cobb-Douglas was good on the basis of R2 (0.748) and f-statistics (17.505). Production elasticity was positive for education, polythene sheet, fertilizers, chemical, irrigation and labour days. Under utilization of resources was found for polythene sheet, fertilizers, chemicals, irrigation and labour days. Over utilization of resources was observed for seed quantity and use of tractor. There exist decreasing return to scale (...

Fida Mohammad, O.S. Abdalla, Sheraz Ahmed, Fakharuddin and S. Rajaram

...haracters except for thousand kernel weight. The influences of yield components on grain yield were explained by the cluster analysis of environments. Clustering of five test environments based on Shifted Multiplicative Model (SHMM) exposed the major role of grains spike-1 and harvest index in production of grain yield. Though the rate and distribution of rainfall during the cropping season were the main contributing factors in the varied performance across ye...

Amjad Ali and Abbas Ullah Jan

...i.e district Mardan, Charsadda and Dera Ismail Khan. Randomly a sample size of 303 sugarcane growers was formally interviewed for crop season 2014-15 through a pretested questioners prepared on the bases of objectives of the study. Stochastic Frontier Approach (SFA) was employed for data analysis using computer software STATA. Result illustrates that technical efficiency of sugarcane crop for the period under study in Khyber...

Muhammad Jamal Nasir, Anwar Saeed Khan, Said Alam and Saeed Akhtar

...n border of district Charsadda, adjacent to Mohmand agency. The construction of dam was started in 2009 and was completed in 2011. It is claimed that dam will irrigate 5000 acre of land in four mouza’s of district Charsadda. Present study is an attempt to determine the impact of Palai dam on the land use and cropping pattern, crop production and revenue of mouza Qilla, Tehsil Tangi, district Char<...

Hina Fatima, Lal Almas and Bushra Yasmin

... analysis. It is indispensable that policies aimed at adapting innovative farming techniques must be implemented in an efficient manner. Effectively implementation of agricultural schemes, formal education and in-formal education should be realized. During and after conducting the agricultural research, it is needed to recommend the farmers about the rate of inputs application i.e. seed, pesticide spray and NPK that are best suited to the local weather pattern...

Naushad Khan1*, Shahnaz Akhtar1, Munir Khan1, Shaista Naz2, Javeria Tanveer1 and Muhammad Kaleem3

...a, Pakistan. For this, a sample of 226 farmers was selected by employing multi stage sampling technique. At first stage, all the three tehsils (i.e.; Mardan, Takht Bhai and Katlang) of the district were selected. At second stage, two villages from each tehsil were randomly selected. In third stage, all the beneficiaries of ZTBL credit program for maize crop was selected and interviewed. Descriptive statistics, paired t-test ...

Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...using the dual culture assay. Among them, Aspergillus flavus was found active fungus against the selected vascular Wilt causing fungus and bacteria, i.e. Fusarium oxysporum (87.50 ± 0.11% inhibition), Xanthomonas campestris (67.00 ± 0.46% inhibition) and Clavibacter michiganensis (72.00 ± 1.02% inhibition). The A. flavus was grown on two different media, i.e. Potato Dextrose Broth (PDB) and Glucose Nutri...

Muhammad Afzal1 and Murad Khan2*

...n the basis of purposive sampling technique. From these tehsils, 92 farms were selected randomly from 460 poultry farms. The sample farms were categorized into three groups i.e. small, medium and large. For analysing the data, descriptive statistics and gross margin were used. The average total cost of small poultry farm was Rs. 3,10,067 per flock while that of medium and large farms were Rs. 4,99,002 and Rs.7,34,290 respect...

Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3 

... agents by trypan blue assay, apoptosis inducing ability by annexin V and JC-1 staining and ultrastructural alterations by transmission electron microscopy. Our data clearly shows that ceranib-2 is more effective than C2 ceramide in promoting apoptosis. The cytotoxic effects of these components cause ultrastructural changes in A549 cells.
...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
...nd species level is necessary for effective antifungal therapy, and can also facilitate control of infections. A total of 50 isolates were isolated from 60 swab samples collected from two poultry processing plants represented broiler carcasses swabs and worker (hand swabs and nail scrapings). Phenotype-based methods for identifying yeast especially Candida species are often difficult, time-consuming and not enough to identif...
Muhammad Arshad1*, Hafiz Azhar Ali Khan2*, Faisal Hafeez3, Ravaid Sherazi1 and Naeem Iqbal4
...nd free choice feeding assays. In the no choice feeding assay, the stages of beetle (adults, 3rd and 4th instar) consumed more aphids than early stages (1st and 2nd instar). In the free choice feeding assay, the consumption of pea aphid (77.647) was statistically high followed by spinach (66.276), coriander (66.14) and cabbage (61.48) aphids. Man...
Önder Duysak*andKübra Azdural
...ted from eight different sampling stations of Iskenderun Bay. The concentration of heavy metal in P. caerulea tissues tended to vary significantly between season and stations.The distribution of heavy metals in P. caerulea followed in their muscular tissues in order; Çevlik > Kaleköy> Payas > Arsuz > Dörtyol > Konacık > Yumurtalık > İskenderun for spring and Kaleköy > Payas > İs...
Waheed Anwar*, Muhammad S. Haider, Ahmad A. Shahid, Hamid Mushtaq, Usman Hameed, Muhammad Zia Ur Rehman and Muhammad Javed Iqbal
... zones of Pakistan. Fusarium equiseti, Fusarium solani, Fusarium incarnatum and Fusarium sp. along with other fungi were isolated and characterized morphologically as well as genetically by amplifying internal transcribed spacer region (ITS). Variability among Fusarium species based o...
Muge Aliye Hekimoglu*, Kgrşat Fırat, Şahin Saka, Cüney Suzer, Aysun Kop and Yaşar Durmaz
... C) was fed on control basal diet (34.93±29.07 mg.g-1). Total carotenoid content of fish skin was determined at30-day intervals. At the end of the experiment the highest weight gain was found to be 1.73±0.37g in Group B, whereas the lowest performance (1.29±0.38 g) was received in Group C. The best feed conversion ratio was found in at Group B. The total carotenoid content of skin of fish were found to be 0.77±0.61 µ...
Serkan Sayiner1* and Hilal Karagul2
...uate. Heifer blood, feed samples including concentrate feed or roughage were analysed. It was found that there are significant seasonal differences for each region and regional differences in each season were due to different feeding conditions. Heifer blood selenium and plasma vitamin E levels were generally found within adequate ranges in all regions for both seasons. We suggest that feed supplements containing selenium should be added for the Iskele region ...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...ut some larvae share the same haplotypes as adults. These findings clearly indicate that the larvae collected from sea waters around an oyster farm belong to P. websteri, the same species as the adult worms collected from the oyster Crassostrea hongkongensis in that locality. Two polydorid-specific primers were successfully designed, for the first time, to amplify target fragments of the COI gene. This study is...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
... for the treatment of dorsal patellar fixation in bovine.
...
Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*
...estic animals. Some microsatellites related to the fecundity of domestic animals have been identified. In the current study, we investigated five fecundity related microsatellites, including BM1329, BM143, LSCV043, OarHH35, and OarHH55, which had been identified in sheep. We estimated their diversity and population structure in three high fecundity goats (Jianzhou big ear goat, Jining grey goat, Dazu black goat) and three lo...
Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2
...e-linked immunosorbent assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for small ruminants was 97.83% (95% CI: 92.35% to 99.67%) and 93.75% (95% CI: 87.54% to 97.44%) respectively. In field this ...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...they also help us to envisage potential efforts to avoid future outbreaks in wild birds, as well as in commercial and backyard poultry.
...

Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns

 

... justify;">Infectious bursal disease virus (IBDV) belongs to the genus Avibirnavirus, a dsRNA virus that causes an acute, highly contagious and immunosuppressive disease in young chickens. The first line of defence against the virus is innate immunity whose activation is independent of the antigen and depends on the ability of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction induces the production of cytokine...

Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki

... in Nigeria, 3,288 serum samples were collected from domestic pigs from 10 states and analysed for anti-tick antibodies using the recombinant TSGP1 indirect ELISA. Of the samples analysed, 13.4% (442/3,288) showed moderate to high reactivity, suggestive of domestic pigs’ exposure to tick-bite. Antibody reactivity’s were found in eight of the ...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
...r. A direct behavioral assay was conducted to investigate the dietary preference and effects of different diets on the fecundity, sex ratio and longevity of B. hebetor. Three different diets [Honey syrup, sugar syrup, date syrup and a control (water)] were used with different solution percentage (25, 50, 75 and 90%). It was observed that honey fed wasp pair produces significantly more number of eggs, adults and also lived significantly longer than other...
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
... and larvae (n=550). The samples were collected from four (Shuba Bazaar, Tarnab Farm, Wazir Bagh and Hayat Abad) dengue suspected locations of district Peshawar, Khyber Pakhtunkhwa (KPK), Pakistan. A semi-nested multiplex PCR was used to amplify the cDNA of the four dengue serotypes. Six out of 35 (17.14%) adult mosquito pools were found positive for dengue virus with type-2 (77.77 %) and type-3 (11.11 %) while a single (11.11%) pool of concurrent infection wi...
Muhammad Afzal1,Naveeda Akhtar Qureshi1,*, Khalid Zamir Rasib2 and Irfan Hussain1
...n choice and no-choice assays conducted under laboratory and field conditions. Visual rating according to (AWPA, 1997) scale, mass loss, wood consumption per termite and mortality rate were determined to evaluate resistance of wood against H. indicola. Populus euramericana and Dalbergia sissoo were observed most palatable and resistant wood species, respectively, in no-choice laboratory and field trials. Five different wooden pairs were ev...
Zafrullah Khan1, Shah Alam Khan1*, Hamayoon Khan2, Naeem Khan3, Khwaja Junaid1 and Inamullah Khan1
...ther wheat cultivars (Pirsabaq-2004, Saleem-2000, Atta-Habib, Khushal-69, Sarhad-82 and Khyber–87) also did not show any antixenosis or tolerance category of resistance to the R. padi. This study concludes, Serin-2010’ as standard resistant cultivar for future breeding program.
...
Md. Yeamin Hossain1,*, Md. Alomgir Hossen1, Md. Nasir Uddin Pramanik1, Fairuz Nawer1, Md. Mosaddequr Rahman2, Suraiya Sharmin1, Dalia Khatun1, A. H. Bahkali3, Abdallah M. Elgorban3 and Khairun Yahya4
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
...survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey’s multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h and 140 µM for 48 h applications. Used as a positive control, 250 µM 5-Fluorouracil decreased the cell survival by only 40 % after 48 h, but li...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...elevation in alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine which indicate renal and liver damage. Concentration of Na, K, Ca, Mg, P, Cu and zinc in serum were significantly decreased (P< 0.05), indicating tubular damage in kidneys. 
...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
... susceptibility.The milk samples were screened with surf field mastitis test and furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen t...
Abdur Rahim1, Ghulam Abbas1,*, Sara Ferrando2, Lorenzo Gallus2 and Abdul Ghaffar3
...mental ponds remained as salinity (15‰ -20‰), dissolved oxygen (5.6 to 7.5ml/l), temperature (25°C to 28°C). pH (7.6-7.8), ammonia (NH4-N) and `nitrites (NO2-N) less than (0.001mg/l), Secchi’s disc visibility (8 cm to 46 cm). Higher percent weight gain (2665 g), specific growth rate (2.76±0.5 % day-1), best feed conversion ratio (0.14), condition factor (4.1), viscerosomatic index (...

 Ali Zurwan1,*, Muhammad Moaeen-ud-Din2, Ghulam Bilal2, Zia-ur-Rehman1,2 and Muhammad Sajjad Khan1

Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...p were injected with the same volume of normal saline. The mRNA and protein expression levels of Ldh-c in plateau pika skeletal muscles were determined by real-time PCR and Western blot. The LDH activities, lactate contents and ATP levels in skeletal muscle were compared between the experimental group and the control group. The results showed that 1) the expression levels of Ldh-c mRNA and protein were 0.804&pl...
Mousa Keshavarz1*, Ehsan Kamrani1, Narges Amrollahi Biuki1 and Hossein Zamani2
...males, females, unsexual samples were counted for 71, 126 and 20, respectively. Females were totally 1.77 times heavier than males. Wet weights of gonads were nearly 8.25% of the mean total wet body weights. Only females were observed by gonad wet weights more than 12 g. The gonad wet weights of females (3.74±3.26) was heavier than males (3.46±2.63). March to August was the spawning months, as a U-shaped trend by August (mid-summer) as its concav...
Shazia Ali, Hizb Ullah and Sarwat Jahan*
...dl) alongwith hormonal assay of GH (ng/mL), T3 (ng/mL), T4 (µg/dL), TSH (μIU/mL), and kisspeptin (ng/ml) was done. BMI and hemoglobin were significantly reduced (P<0.001), while serum ferritin were significantly increased (P<0.001) in all four thalassemic groups on comparison with control. Kisspeptin levels were significantly reduced in ≤13 years female (P<0.001). While significantly high levels were observ...
Fermín Lopez-Uriostegui1, Jesús T. Ponce-Palafox2,*, Fabiola Lango-Reynoso1, María del Refugio Castañeda-Chavez1, Virgilio Eugenio Arenas-Fuentes3 and Fernando Vega-Villasante4
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... sectioned in the median sagittal plane and histological slides of the worms were prepared for detailed morphological studies. Bile tissues, aspirates and blood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morphological identification of G. expalanatum was carried out on the ba...
Andrea Rezic1, Ivica Boskovic2, Piera Lubinu3, Marina Piria1, Tihomir Florijancic2, Massimo Scandura3 and Nikica Sprem1*
...ndmarks described the dorsal side of the skull and lateral side of the mandible. Individual variation in skull and mandible morphology was statistically significant for the shape (p<0.01). Sexual dimorphism as main effect was highly significant for the dorsal skull shape and the mandible shape and size (p<0.01). The comparison of mandible mean shapes using discriminant function analysis and parametric test did not reve...
Mingqin Shao1*, Bin Chen1, Peng Cui2, Binbin Zeng1 and Jianhong Jiang1
... Future studies are necessary, and the main goal should be focused on sex ratio, mortality and sex ratio at birth.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...pulation. A total of 412 samples which included 148 myocardial infarction survivors, 162 Normolipidemic healthy controls and 102 patients with hypercholesterolemia in a   Indian Tamilian population were included in the study. Genotyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regres...
Zafar Iqbal1* and Muhammad Khurshid2 
...-PCR (IC-PCR) is very versatile technique that has the potential to expedite the detection of plant viruses from plant species containing various forms of PCR amplification inhibitors. In current study, IC-PCR was used for the detection of Tomato leaf curl New Delhi virus (ToLCNDV) from plant extracts of Nicotiana benthamiana plants inoculated with ToLCNDV. To immunocapture the ToLCNDV, two antisera raised against Tomato yellow leaf curl virus-co...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...ce, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup A (ALV-A) infection was confirmed by necropsy, histopathological examinations and PCR analysis. The results showed, 20 chickens as positive for ALV-A (2.73%, 95% CI 1.7-4.2) and these chickens ...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3

 

...il (14% vs 5%). Only one sample was PCR positive. The results show that overall prevalence of Toxoplasma gondii infection in young women of Rafha city is low as compared to other regions of Saudi Arabia.
...

Tuli Dey, Sonnet Poddar and Mukti Barua

...ully that there could be saved the life without any complication. The prolapsed mass of cow was managed with lukewarm water and manual pressure replacement in non-raising condition of hindquarter. Vulvar Retention Suture (modified Bhuners suture) was given for not to recurrence of the condition. Proper postoperative treatment with antibiotic, antihistaminic and hormonal drugs were given. This case as well as the condition of the animal were followed up and gav...

 Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy

...dred swabs and 500 organ samples were collected from apparently healthy and freshly dead quails respectively. Bacterial isolation and characterization were performed in accordance with the clinical laboratory standards and confirmed by PCR. In life birds, only E.coli and Salmonella could be recovered from Cloacal swabs, while in freshly dead birds all four pathogens disseminated in various organs with higher incidence of mixed compared to single infection. Dif...

Zahir Shah, Ahmed Nawaz Tunio, Shakoor Ahmad, Ijaz Ahmad, Javid Ali, Sher Bhader Khan

...urs was subjected for caesarean section at Veterinary Teaching Hospital, Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, University of Agriculture Peshawar-Pakistan. The surgery was performed under paravertebral anesthesia at T13, L1-3 using xylocaine 2% in standing position after proper sedation. The operated site was prepared for aseptic surgical procedure. A vertical incision was given in middle of the left flank region. Tw...

Iftikhar Ahmad, Abdul M. Saleem, Ghulam Mustafa, Khurram Ziaf, Irfan Afzal and Muhammad Qasim

...a elegans were primed in salt solutions and distilled water for 24 h and 12 h, respectively, and germinated at 25 ± 1 °C with 40-60% R.H. in 16/8 hours day/night conditions. Results revealed that pre-sowing seed treatment particularly halopriming with CaCl2 was the most effective for invigoration of gerbera and zinnia seeds. For Gerbera jamesonii, 25 mM CaCl2 and for Zinnia elegans, 50 mM CaCl2 proved best priming treatments followed by 100 mM KCl a...

Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1

... poultry carcass.Several samples were collected from three poultry slaughterhouses within Jos metropolis at intervals for a period of 6 months to check for presence of Salmonella during various stages of processing. Salmonella was isolated from at least one of the four matrices in the three LBM with an overall prevalence of 20.2%. Of the 84 samples collected, 17 contained Salmonella. With regard to the matrices, there was no...
Abdul Malik1,4, Ghulam Abbas1*, Hameeda Kalhoro2, Illahi Bux Kalhoro3, Syed Sajjad A. Shah4 and Halima Kalhoro3 
...e the effects of varying salinity levels (0‰-30‰ with 5% increment) on breeding of red tilapia. Brood fish were stocked in hapa nets suspended in cemented rectangular tanks (11×7×4 feet) . Three females (mean weight 145 g) and one male (140 g) were kept per tank with 2 replications. They were fed with commercial floating pellets having 35% crude protein at 2% body weight twice a day for 60 days and eggs were collected weekly. Results ...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
...were dissolved in normal saline. Of this one part was taken for thaw and freeze treatment, while the second for ultrasonication treatment, before conducting antimicrobial activities. Zones of inhibition were measured by using disc diffusion method. Supernatant fraction of E. coli was positive against Sal. typhi and supernatant fraction of P. multocida was positive against E. coli, which show that antimicrobial a...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Rassol Bakhsh Tareen2, AsadUllah1 and Zahid Mustafa1
...bs i.e. Allium sativum, Cassia angustifolia and Artemisia scoparia @ 0.5 and 1.0% respectively, control (basal diet), and a positive control (Oxyfeed® @ 2g/kg). In the study, the treatment groups, which were given diet supplemented with herb and antibiotic, exhibited improved weight gain, average daily gain, and feed conversion (P<0.05). No significant difference was observed in ...
Nadeem Rashid1,*, Masroor Ahmad Bajwa1, Mohammad Masood Tariq1, Tanvir Ahmad2, Majed Rafeeq1, Zafar Ahmad1, Mohammad Arif Awan1, Mohammad Ali1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Asad Ulah1 and Amanullah Khan3
... commercial broiler feed samples (n=96) were collected from broiler chicken farms in and around Quetta district. Physical properties i.e. moisture and pH were determined by oven drying and pH meter, respectively. Viable fungal count of the feed samples was estimated by using spread plate technique. Identification of fungal isolates was carried out on the basis of cultural and morphological characters by using slide cu...
Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
...ondria , chromatin condensation, mitochondrial vesiculation and dispersion at ooplasm were observed. In mitochondria, mitotic catastrophe , vesiculation, swelling and loss of organization of cristae were detected.Here we showed that TiO2 exposure trigger paraptotic type cell death in zebrafish ovary.
...
Sehrish Rasool1, Zain ul Abdin1,*, Saqi Kosar Abbas2, Sumra Ashraf1, Maryam Anwer1, Atif Manzoor1, Muhammad Tahir1 and Hoor Shaina1

 Saqib Ali1, Suliman Ali1, Lina1, Wen Zhou1, Muhammad Irfan Waris1, Ashfaq Ali2 and Man Qun Wang1,*

...lantation of trap trees, sanitation, removal of the damaged trees and exact placement of insecticide saturated sticks into larval sites can reduce the spread of ALB in the regions of China. The ecological, in addition to biological uniqueness and development of sawyer beetles (Monochamus alternates) are also discussed.
...
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
...tan. A total of399 serum samples from indeginous and exotic cattle were tested for brucellosis by RBPT, SAT and multiplex real-time PCR for Brucella sp., Brucella abortus and Brucella melitensis. A total of 20 (5.01%) samples were found seropositive by RBPT and 19 (4.76%) by SA...
Wali Khan*, Ghazal Mumtaz, Saima Bibi and Salma Afzal
...A total of 520 vegetable samples were collected during April to October 2016. These vegetable samples were screened for parasitic contamination. The results showed that 10.7% of the vegetables were found to be contaminated with one or more than one species of parasites. Intestinal parasites Ascaris lumbricoides 26.7%, Trichuris trichura 19.6%, Taenia saginata 25 % and Entamoeba histolytica...
Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2
...to determine the main causative serotype of 2013 DENV outbreak in Pakistan. Overall 703 serologically positive suspected patients from different major health centers of Pakistan were registered in present study of which 214 were females and 489 were male. Overall weighted prevalence of PCR positivity was 38% (268). Of the total 268 PCR positive subjects, 0.74%, 56.71%, 15.67% were positive for serotype 1, 2 and 3 respectively. Only 72 suspected patients had co...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...an. A total of 120 serum samples were randomly collected from buffaloes and cattle (60 per species) at Civil Veterinary Hospitals, animal markets and peri-urban livestock holdings in Rawalpindi and Islamabad. Serum samples were initially screened by the Rose Bengal Plate Test (RBPT). RBPT positive samples were subjected to a B. abortus specific indirect enzyme-linked immunosorbent a...
Memoona Akber1, Muhammad Mushtaq1,*, Muhammad Sajid Nadeem1, Amjad Rashid Kayani1 and Noor-un-Nisa2 
..., live trapping of commensal rodents was carried out from May 2011 through April 2012. A total of 100 rats (R. norvegicus = 65, Rattus rattus = 35) were trapped during 720 trap nights @ 0.13 captures per night and the trap success was 13.88%. Maximum captures of the Norway rats were recorded during summer (n = 33), followed by winter (n = 13), autumn (n = 12) and the least number was recorded during spring (n = 7). A non-significant difference be...

Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5

... virus collected from bursa of clinically infected chicks, No morbidity and mortality were observed. During present study, some milder infection with field strain of IBDV almost a week ago recorded were also found in the control group when challenged with IBDV, IBD vaccines in the presence of specific maternally antibodies of IBD could no induce antibodies titer 1500 with live vaccines were protective against the IBDV. Based on the findings of present study, i...
Irum, Akram Shah*, Sobia Wahid, Nazma Habib Khan and Qaisar Jamal
...s severe morbidity and disability among effected populations. Pakistan was declared dracunculaisis-free in 1996. The present study is a follow-on to the declared eradication of dracunculiasis in Dera Ismail Khan district of Khyber Pakhtunkhwa (KP) Province, Pakistan through investigating disease recurrences and Cyclops species in drinking water sources. A cross-sectional household survey was carried out using in-person questionnaire evaluation along wit...

Muhammad Waqas1*, Anwar Ali Shad1, Omaira Bashir2 and Mohsin Iqbal3

...ling index were remained same at different level of oil carotenoids tomato peel powder when compared with control. On the other hand spaghetti cooking becomes decrease by increase of carotenoids level in spaghetti when compared with control. Organoleptic evaluation of spaghetti prepared from lycopene revealed maximum score of smell, taste, odors, color texture and overall acceptability when compared with other tested sample ...

John G. Bruno

...linked aptamer sorbent assay (ELASA) screening were shown to specifically bind their cognate antibodies when captured by their Fc tails on the surface of Protein A-conjugated magnetic beads. While no clear similarities in the partial or complete primary DNA sequences or secondary stem-loop structures of these aptamers were observed, 3-dimensional computer models of these top ten anti-idiot...

Muhammad Zafarullah Khan

...lected from the selected sample of AEOs by mailing the questionnaires. The results showed that overwhelming majority of the respondents i.e., 88% were M.Sc degree holders. They have had an average work experience of 16 years. Significant differences were observed among the competencies of AEOs with their participation in training programs and their higher educational qualification. The differences were considered as training needs and the AEOs need in-service ...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...es is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was to develop H9 plasmid-based DNA vaccine against the currently circulating AIV subtype H9N2 targeting HA gene of the virus A/Turkey/Egypt/1341V/2013(H9N2). The full length sequence of HA gene was codon optimized to the chicken biased codons and subcloned into the pCAGGS plasmid vector under the control of the chicken β-actin promoter. The in vitro express...

Waqar Islam

...ectors can reduce this disastrous problem. However, in developing countries such strategies are rarely applied due to lack of farmer’s knowledge about plant virus diseases. Therefore, here we reviewed about this issue by highlighting that what farmers know about plant viruses, what is their perception about yield losses by virus diseases and what management strategies they choose to apply against viruses. We further added our suggestions that how plant v...

Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*

...oplasma spp. from tissue samples collected from clinically sick (60 samples) and apparently healthy broiler birds (30 samples) in a commercial farm, using classical isolation and compared with molecular identification of Mycoplasma infections. A total of 96 tissue samples from trachea, air sacs, lungs and oral swabs al...

Sadiq Ali* and Ijaz Ahmad Khan

...lon (L.) Pers., Cannabis sativa L. and Chenopodium album L. with relative density of 11.37, 10.86 and 7.31%, respectively. Moreover, at different locations, the parthenium weed is competing with C. sativa which is not problematic like parthenium weed and replaced by the latter. Mean distribution of parthenium weed infestation was abundant and almost not uniform in all sites, because some sites were in hilly area. The compute...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...insights into past dispersal events in natural populations of this species. We investigated the molecular phylogenetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. domestics is a newly arrived subspecies currently restricted to Shanghai, indicating ...
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
... China. Furthermore, the samples all from Southwest China in the GX group may be an example of ‘refuge within refugia’ in glacial period. According to our molecular clock analysis, the main clades of M. fortis divergence and separated time at around 0.77±0.64 million years ago (Mya) located in the Penultimate Glaciation. Divergences within the three clades taken place during the interglacial period between the Penultimate Glaciation a...
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...n. After 2 months, MTT assay showed significant differences in the proportion of adherent viable cells in the FH group and GSH group compared with the control group (p < 0.05), and the FH+H group had a more beneficial effect on post-thaw survival of cryopreserved ADSCs compared with the GSH+H and GSH+FH+H groups. The use of FH and H in long-term storage has the potential to be helpful in commercial and clinical application of ADSCs.
...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
... Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of varianc...
Urooj Fatima1, Arslan Mehboob2, Muhammad Abid3, Muti-ur-rehman Khan4 and Tahir Yaqub4*

 

...kistan, a total of fifty samples from sick dogs suspected for parvovirus infection were collected from different geographic areas of the country. Samples were screened for positivity and verified for CPV using PCR. From a 30% positive samples for hemagglutination, nineteen (38.4%) samples were positive in PCR. Sequence analysis of the selected positive sa

Neelum Andaleeb, Munir Khan and Syed Attaullah Shah

...ticipation. A multistage sampling method was used to select a sample of 274 livestock farm households from 6 rural Union Councils, and a well-structured questionnaire was used to collect data from female respondents through face to face interviews. A composite index was constructed to measure the extent of women’s participation, and a linear regression model was used to determine factors affecting women’s involve...
Muhammad Tahirand Memoona Shehzadi*
...rsity of Agriculture, Faisalabad, Pakistan. The treatments comprised on the combination of chemical fertilizers (No chemical fertilizer, half recommended dose of fertilizer and full recommended dose of chemical fertilizer) and Plant Growth Promoting Rhizobacteria (PGPR) (No PGPR, PGPR-1, PGPR-2 and PGPR-1 + PGPR-2). The PGPR used in this study were pre-isolated and characterized, taken from soil microbiology and biochemistry laboratory, Institute of Soil and E...

Wazha Mugabe1,2, Lawrence Akanyang1, Mackenzie Nsinamwa1, Batanani Moatswi1, Naledi Matthews1, Kealeboga Dipheko1, Imtiaz Ahmed Ujjan3 and Assar Ali Shah2*

Waqas Ahmed Dogar2, Arshad Ali Khan3*, Saeed Ahmed2, Sudheer Tariq2, Mukhtar Ahmad2, Muhammad Imran2, Muhammad Noman2 and Nadeem Khan1 

... of citrus (Kinnow and Musambi) grown under drip irrigation at Postgraduate Agriculture Research Station (PARS) Institute of Horticultural Sciences, University of Agriculture Faisalabad. Plants were grown at three planting distances as T1 (11 x 22ft), T2 (11 x 11ft) and T3 (22 x 22ft). T1 (11x22ft) was optimum plantation and showed best results for yield. Maximum fruit yield per plant (3.6373 kg) was observed from the
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
Ahmad Khan1, Raza Ullah Khan1*, Muhammad Zameer Khan1, Fayyaz Hussain1,Muhammad Ehsan Akhtar2, Jalal-Ud-Din3
...rate of 20 kg/ha. Almost same trend was observed with respect to the number of bulbs per plot. The highest number of 28.3 bulbs/plot were obtained in CDHA given at 20 kg/ha, followed by 25.5 and 24.6 bulbs/plot in SFDHSs and MDHSs both applied at 30 kg/respectively. On overall, results from this study implies it is optimum and economical to apply CDHA at 20 kg/ha whereas SFDHSs and MDHSs at 30-40 kg/ha for study area and vegetable.
...
Said Rawan1, Farzana bibi1, Nadeem Khan1*, Abdul Mateen Khattak1, Zahir shah3, Asif Iqbal2, Madeeha Alamzeb2, Saeed Ul Haq1, Abid Kamal3, Fawad Ali Shah2, Ahmad Naeem1 and Wajid Ali3
... loss (%). The fruits of safeda variety lowest fruit decay (5.82%), more TSS (11.530Brix), taste (5.17) and moisture content (42.68%), while fruit store for zero days no fruit decay (%), weight loss (%), less firmness(1.43 kg/cm2) and more moisture content (44.24%), however on 9 days storage more TSS (15.130Brix) and taste (6) was observed. The fruit store for 9 days and safeda variety perfor...
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...iver induced by D-Galactosamine (D-GaIN) in rats. With this in mind, the rats were categorized into five groups. The study groups were given saline, 0.2 % dimethyl sulfoxide, D-GaIN, EA plus D-GaIN and D-GaIN plus EA, respectively. In the group given D-GaIN, the following transmission electron microscopic and light microscopic results were found: degenerative changes in the liver tissue, significant decreased in the number o...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...amily: Arial, Helvetica, sans-serif; font-size: 12px; text-align: justify;">The detection and identification of viruses has been a challenge since the advent of the discipline of plant virology over a century ago.Agreat variety of methods have been developed that permit differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based o...

M. B. Meah

Biopesticides for crop growth and crop protection
...amily: Arial, Helvetica, sans-serif; font-size: 12px; text-align: justify;">Tablets made of garlic bulbs and allamanda leaves tremendously increased germination and reduced damping-off, seed rot, seedling blight and tip over of vegetables (egg-plant, tomato, chilli) in the nursery and leaf blight, anthracnose, fruit rot, root knot and leaf curl/mosaic of tomato and carrot in the field. Aqueous solution of garlic and allamand...

 Sitansu Pan and Amrita Das

Evaluation of shelf life of some value added organic formulations of Trichoderma harzianum
...amily: Arial, Helvetica, sans-serif; font-size: 12px; text-align: justify;">Shelf life of Trichoderma harzianum (Th AN) was studied during 2007-08 in some organic formulations like vermicompost, leaf manure, rice bran and FYMand also their combinations with two oil cakes, neem and mustard cake. The growth was estimated in terms of colony forming unit (c.f.u.)/g of substrate up to 120 days at 30 days interval. In all organic products or their amendment with oil...

Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram 

Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...five leaf and nine fruit samples of chilli were collected from different regions of Karnataka for isolation of chilli microflora. From these samples, 94 fungal and 89 bacterial isolates were derived, which included 50 isolates of fungi and 44 isolates of bacteria from phylloplane and pomoplane, 44 isolates of endophytic fungi and 45 of endophytic bacteria from inside the leaf and fruit tissues, respectively. Among them, 70 f...

Neerja Agrawal, Mukesh Srivastava, Akhilesh Tripathi and Amrendra Singh

Survey and monitoring of pests, parasites and predators of pulse crops in central and eastern Uttar Pradesh
...lume moth Exelastis atomosa, tur pod bug Clavigralla gibbosa, jassid Amrsca bigutulta, termite Odontotermes spp, green bug Nezara viridula, tur podfly Melanogromyza obtusa, blue butterfly Lampides boeticus, cow bug Tricentrus bicolor and Pentatomorpha bug were recorded while on lentil crop, black aphid,Aphis craccivora was recorded as major pest and H. armigera and cut worm Agrotis ipsilon...

K.N. Ahmed, S.H.A. Pramanik, M. Khatun A. Nargis M. R. Hasan

Efficacy of plant extracts in the suppression of insect pests and their effect on the yield of sunflower crop under different climatic conditions
...e of Neem seed oil and sesame oil, leaf extracts of custard apple (Annona squamosa ) and Bara Bishkatali ( Polygonum orienta) were tested against the pest infestation. The treatment of custard apple leaf extract produced the most favourable result in respect of pest control and crop yield. The other treatments also exhibited better results in comparison to control followed by Bara Bishkatali, Neem seed oil, a mixture of Neem...

Sitansu Pan, Surojit Khalko and Amrita Das

Effect of some fungicides on seed mycoflora, germination, viability and their persistence in treated seeds
...ra oryzae in rice (Oryza sativa L.). Propineb 70WP and prochloraz 45EC considerably reduced the appearance of A. alternata and flusilazole 40EC reduced Fusarium sp.in mungbean seed than untreated control.Amarked reduction in population of A. brassicae by difenoconazole 25EC and flusilazole 40EC was observed in mustard seeds. Inhibitory effect of fungicides on seed germination of all tested seeds was observed with prochloraz ...

A. K.Bhattacherjee and B. K. Pandey

Dissipation of carbendazim in mango after pre- and post-harvest treatments
... to mango fruit (cv. Chausa) at 10 days interval in such a way that last spray fell 12 days prior to harvest to control post harvest diseases viz., anthracnose (Colletotrichum gloeosporioides) and stem end rot (Lasiodiplodia theobromae). Carbendazim dissipated to 1.01 and 2.14 mg kg in mature whole fruit after harvest (12 days after second spray) at 0.05 and 0.1 per cent concentrations, respectively, following first order rate kinetics. The corresponding value...

1N. Johnson Singh, 2Amitava Konar and 3Palash Mondal

Incidence of aphids in different traps on potato in Gangetic plains of West Bengal
...006-07 and 2007-08 at Adisaptagram Block Seed Farm, Hooghly,West Bengal to evaluate the population dynamics of aphids with the help of yellow pan trap and yellow sticky trap. The population of aphid catches was quite lower in yellow pan trap than yellow sticky trap. Aphids were first observed in traps during third week of December and the population of pests gradually increased to reach the peak in second week of February in the first year of study (2006-07) w...

B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran

Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...rsely and to prevent the sale of poor quality products to the farmers, the Central Insecticide Board of Government of India has made registration of microbial pesticides mandatory before commercial production/import/sale. Guidelines and data requirements for registration of microbial pesticides have been provided in the annexure of Insecticide Act. Quality control parameters set by CIB are inadequate for knowing potentiality...

1Parvin Noor and 2Md. Nashir Uddin

Morphological changes of different castes of the subterranean termites (Odontotermes proformosanus) in fungus combs of termitophiles
...hat Odontotermes proformosanus are polymorphic in nature and have exopterygote post-metamorphic development. During the period of study, eggs and nymphs were collected in different seasons from the fungus combs of termite mounds. Eggs of O. proformosanus were found profusely in fungus comb in the months of March, May, September and October of 2006 to 2008 Average lengths and standard deviation (mean±SD) of the eggs we...

1Bikash Ranjan Ray and 2Mrinal Kanti Dasgupta

Management of root holoparasite Aeginetia pedunculata of (Orobanchaceae), causing wilt of sugarcane by trap and catch crops
...eds in soil. Rice (Oryza sativa L.), maize (Zea mays L.), pearl millet (Pennisetum typhoides Staph. & Hubbard.), italian millet (Setaria italica (L.) Breauv.), black gram (Phaseolus mungo L.), dhaincha (Sesbania aculeata (Willd.) Pers.), sesame (Sesamum indicum L.), jute (Corchorus olitorius L.), pigeon pea (Cajanus cajan (L.) Millsp.), and groundnut (Arachis hypogaea L.) were found ef...

A. K. Chaubey and Satyandra Kumar

Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...gnita and wilt fungus, Fusarium oxysporum f. sp. lycopersici on tomato in laboratory and pot culture experiment. All the isolates were isolated by serial dilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with antagonist fungus and bacteria isol...

Bijan Kumar Das

Incidence of Aleurocanthus spp. (Aleyrodidae: Hemiptera) on betelvine (Piper betle L.) and their interaction with host plants
...ckfly, Aleurocanthus rugosa Singh (Aleyrodidae: Hemiptera) is a major pest causing severe damage to the foliage in the conservatories (borojas) of West Bengal. The seasonal incidence of A. rugosa in boroja was recorded during 2003-2004. A. rugosa adults were active in the boroja throughout the year with two peaks, one in pre-monsoon and another in post-monsoon i.e., period prior initiation...

Bushra Afreen, Geetesh Baghel, Akil A. Khan and Q.A. Naqvi

Virus- vector relationship of yellow vein disease of calendula and the whitefly
...h whiteflies not through sap inoculation. A single whitefly could transmit the virus and showed 20.55% infection and fifteen whiteflies were required for 100 percent transmission. The minimum acquisition access feeding period and minimum inoculation access feeding period for the present virus were 10min and 30min, respectively. Acquisition access feeding period and inoculation access feeding period was 100 percent at 6hr and 3 hr respectively. A pre-acquisitio...

S. K. Dutta, S. Roy Chowdhuri, D. Pandit and A. K. Bajpai

Rot diseases of muga host, Som (Persea bombycina) in Assam, India
...a silkworm, (Antheraea assamensis Helfer) growing mainly in North Eastern region and Uttaranchal State in India. India is unique in producing the golden muga silk and has monopoly in global silk market grows up to 20-25 meters and survives for 18-20 years. Pollarding of Som plant at 6 ft height is a common practice in North Eastern region of India for controlled rearing (outdoor) of muga silkworm. It was observed that stems of som plant (specifically the polla...

Debjani Chowdhury, P. C. Paul and B. Dasgupta

Management of leaf spot of Centella asiatica (Thankuni) caused by Alternaria sp. and target leaf spot of Rauvolfia serpentina (Sarpagandha) caused by Corynespora cassicola

Amitava Konar, Palash Mondal and N. Johnson Singh

Occurrence of potato pests in different dates of planting in Gangetic plains of West Bengal, India
...006-07 and 2007-08 at Adisaptagram Block Seed Farm, Hooghly, West Bengal, India. Kufri Chandramukhi was planted in five dates of planting starting from 3rd week of November with one week interval upto 3rd week of December with a spacing of 60×20 cm. The trial was laid in Randomized Block Design (RBD) with four replications. The recommended practices for raising the crop in the field was maintained except the application of insecticides. It was observed t...

D. Sharmah, K.C. Deka and A.K. Phukan

Field evaluation of efficacy of some botanical dust against rice bug (Leptocorisa oratorius Fabr.)
...t the rice bug (Leptocorisa oratorius Fabr.) during 2000-2001 on the basis of per cent reduction under field condition. The per cent reduction in number of rice bug adults due to neem seed kernel powder, neem seed kernel powder + multani powder, multani powder, neem leaf powder, neem leaf powder + multani powder, neem leaf powder + ash, soap nut kernel powder, soap nut kernel powder + multani powder tested as dust were found to be 24.44, 14.78, 5.65, 17.06, 11...

K.N. Ahmed , M.A. Al-Helal, N-E-P. Khanom, S. Bulbul

Control strategies of papaya mealybug, Paracoccus marginatus Williams & Willink infesting vegetable crops in Bangladesh
...ya mealybug feeds on the sap of the plants by inserting its stylets into the epidermis of the leaf as well as into the fruit and stem. In doing so, it injects a toxic substance into the leaves. The result is chlorosis, plant stunting, leaf deformation, early dropping of the leaves and fruits, a heavy accumulation of honeydew and death of the host plants. Heavy infestation is capable of making fruits inedible due to the deposition of thick white waxy material b...

M. S. A. Mamun and M. Ahmed

Integrated pest management in tea: prospects and future strategies in Bangladesh
... shade regulation, field sanitation, fertilizer application, manipulation or destruction of alternate hosts and selection of pest resistant/tolerant varieties and mechanical mechanisms like manual removal, heat treatments, light traps, use of bio-pesticides, bio-control agents and sex pheromone trap need to be given more importance in pest management programme. A tentative IPM strategy for tea cultivation in Bangladesh has been proposed in this paper. Thus the...

Nilesh Suresh Gole and Bijan Kumar Das

Biology of Dichromia sagitta (Fabricius) (Noctuidae: Lepidoptera), a serious pest of Indian ipecac, Tylophora indica
...foliator pest, Dichromia sagitta (Fabricius) (Noctuidae: Lepidoptera). A study was conducted in the field and laboratory of All India Coordinated Research Project on Medicinal & Aromatic Plants and Betelvine (AICRP on MAPB) at Bidhan Chandra Krishi Viswavidyalaya (BCKV), Kalyani, Nadia, West Bengal during 2010-2011 on seasonality, extent of damage and biologoy of D. sagitta on T. indica. The larvae were active almost thr...

Sitansu Pan and Amrita Das

Control of cowpea (Vigna sinensis) root and collar rot (Rhizoctonia solani) with some organic formulations of Trichoderma harzianum under field condition
... of the cowpea plants, basal girth, number of branches & pod number, pod vigour of the bio primed seeds over other treatment combinations. Soil application of Trichoderma in vermicompost +20% neem cakes gave better disease control over the others.

...

Sunita J. Magar and D.D. Nirmal

Effect of plant spacings and cultivars on spread of Yellow Vein Mosaic Virus (YVMV) disease
...>Spread of yellow vein mosaic virus (YVMV) disease in different plant spacing 30 x 30 cm, 50 x 30 cm, 60 x 30 cm, 60 x 60 cm and 60 x 40 cm and using two cultivars, i.e., cv. Parbhani Kranti and Local indicated that the first appearance of symptom was earlier in cv. Local and late in cv. Parbhani Kranti. The rate of infection was higher at 45 to 60 days after sowing in both kharif and summer while in cv. Local it was higher at 30 to 45 days after sowing. Less ...

Amitava Konar, Kiran A. More and Pradip Mondal

Efficacy of some insecticides against cutworm and molecricket of potato in West Bengal
...) infesting potato at Adisaptagram Block Seed Farm, Mogra, Hooghly, West Bengal (India) during 2007-08 and 2008-09. The field trial was conducted in Randomized Block Design with six treatments and five replications. Among the various treatments for managing soil pests of potato (cv. Kufri Bahar) i.e. cutworm and molecricket soil treatment of phorate 10 G @ 15kg ha-1 at planting plus drenching the ridges with chlorpyriphos 20 EC @ 2.5ml/L of water on appearance...

Amitava Konar, S. Paul and Kiran A. More

Efficacy of different insecticidal treatment schedules against aphid and whitefly on brinjal
...field experiments at 'Adisaptagram Block Seed Farm, Hooghly, West Bengal, India in two consecutive kharif seasons i.e., 2010 and 2011. The experiment was laid in RBD with seven treatment schedules and three replications. Among all the treatments soil application of phorate at transplanting, followed by foliar spray with acephate, thiodicarb and Bacillus thuringiensis var. kurstaki at 50, 70 and 90 days after transplanting, respectively) and seedling treatment ...

A. Regupathy and R. Ayyasamy

Initiatives of papain industry by private-public-farmer link-ages in classical biocontrol program for papaya mealybug in Tamil Nadu
...papaya fields were compensated for the yield loss to serve as field-mass-multiplication-strategy. Further, the farmers of neighbouring fields were encouraged and facilitated through thirteen field staff to take the bug infested leaves and fruits from parasite-released fields and dispersed in new fields. The effective mass-spreading of parasitoid was made thorough door-to-door contact and farmers were monitored not to take up spraying. The infested plant parts ...

S.K. Sahoo

Incidence and management of mustard aphid (Lipaphis ery-simi Kaltenbach) in West Bengal
... the aphid on the yellow sarson variety, Binoy (B-9) was observed from 52nd standard week, with the peak population on 6th standard week and the aphid disappeared after 10th standard week. Among the different chemical insecticides evaluated for their bio-efficacy against L. erysimi, Dimethoate 30EC and Oxydemeton-methyl 25EC were proved to be more effective. The plots treated with Dimethoate and Oxydemeton-methyl recorded mi...

Md Riton Choudhury, Koushik Brahmachari, Sudeshna Kar and Rupayan Deb

Integration of weed management practices in rice-potato-groundnut cropping sequence
...s. From the point of eco-safety measures and economy, combination of hand weeding and mulching can be judiciously recommended in potato and groundnut.


...

Madhuban Gopal, Rajesh Kumar and Arunava Goswami 

Nano-pesticides - A recent approach for pest control
...graphy (HPLC). The bio-assay studies revealed many fold improved activity of nanopesticides against fungi and mites as compared to commercial sulphur.

 

...

M. A. Al-Helal, K. N. Ahmed, N-E-P. Khanom, and S. Bulbul 

Observations on papaya mealybug, Paracoccus marginatus Williams & Willink (Hemiptera : Pseudococcidae) damag-ing some crops in Bangladesh
...gous insect pest sucking saps from the attacking fruits and leaves. P. marginatus passes through egg and three nymphal stages to become adult. Generally, three and four nymphal instars occur in case of female and male mealybug, respectively. The adults are most active in warm, dry and humid weather. The male adult mealybugs possess two pairs of wings. Female have no wings and move by crawling short distances or by being blown in air currents. Female usually la...

Goutam Mondal

Plant growth promoting activity of some indigenous Tricho-derma isolates and their field performance against sheath blight of rice in old alluvial zone of North Bengal
...ant pathogens, namely, Fusarium oxysporum f. sp. ciceri (Padw.) Matuo. and Rhizoctonia solani Kühn. Efficacy in plant growth promoting activity of all the isolates was also tested on cauliflower (Brassica oleracea var botrytis L.), chilli (Capsicum frutescens L.) and tomato (Lycopersicon esculentum Mill.) with culture filtrate of biocontrol agents and found both positive and negative effect on seed germination, plant growth and vigour. Maximum increase in...

M.S. Islam, M.Ali, and I. Ahmad

Efficacy of sedomil 72WP and recozeb 80WP to control die-back (Colletotrichum gloeosporioides) of tea in Bangladesh
...isease of tea and its causal organism.

 

...

Dipak Mandal, Paramita Bhowmik, Pronobesh Halder and M. L. Chatterjee

Determination of relative toxicity and base line data of dif-ferent insecticides against cotton mealybug (Phenacoccus solenopsis Tinsley)
...ustify;">Laboratory bioassay was conducted to determine the toxicity of some insecticides and their LC50 values against 3rd instar nymphs of cotton mealybug, Phenacoccus solenopsis in the Department of Agricultural Entomology, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Mohanpur during 2012. Both treated food untreated insect and treated food treated insect methods were followed. The lowest LC50 values through treated food untreated insect method were recorde...

D. C. Khatua, B. Mondal and G. Saha

Bioassay of some agricultural chemicals, human drugs and disinfectants on Phytophthora melonis Katsura causing fruit and vine rot of pointed gourd

S. Patra, V.W. Dhote, SK F. Alam, B.C. Das, M.L. Chatterjee and A. Samanta

Population dynamics of major insect pests and their natural enemies on cabbage under new alluvial zone of West Bengal
...nd 10.50% larval parasitisation during both the seasons, respectively, whereas maximum coccinellid was observed on 23rd February of 2011-12 and 2012-13 crop seasons with 11.67 and 9.67 coccinellids/ 5plants, respectively. Both maximum and minimum temperature had major role to build up the population of diamond back moth, C. plutellae and coccinellids beetle while aphid population was enhanced only by maximum temperature. Relative humidity and rainfall had nega...

D.K. Hazra, Megha Pant, S.K. Raza and P. K. Patanjali

 

Formulation technology: key parameters for food safety with respect to agrochemicals use in crop protection
...tions) and dose rates of same pesticides applied to improve food quality with minimum pesticide residues. 

...

sans-serif;">Shams Ur Rehman, Muhammad Arif and Abdul Mateen 

 

Soil-borne viruses in major potato growing areas of Pakistan
...bait test, infectivity assays and double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Both the viruses were prevalent in Hazara and Malakand divisions. The average symptoms based incidence in Hazara divi-sion for PMTV was 8.83% and TRV was 6.80% while in Malakand division the average symptoms based ...

Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha

Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
... as Stein-ernema carpocapsae. This was further confirmed by ITS-rDNA sequences analysis. Phylogenetic was constructed for studying relationship with known isolates. Pathogenic potential of the isolate of S. carpocapsae (IISR-EPN 06) on the larva of shoot borer (Conogethes punctiferalis), hairy caterpillar (Euproctis sp.) and greater wax moth (Galleria mellonella) was found under in vitro condition. Further this isolate displ...

Anjan Bhattacharyya, Suhrid Ranjan Barik & Pritam Ganguly

New pesticide molecules, formulation technology and uses: Present status and future challenges
... are going on to develop safer molecules which could undergo photo-degradation, microbial degradation as well as chemical degradation leaving very less amount of residues in the environment. Conventional pesticide formulations like WP, EC etc are endangering human health and polluting environment. Newly developed modern formulations like water emulsifiable gel, floating granules, drift less dust, macro and micro encapsulated suspension, hollow fibers, monolith...

Amitava Konar and N. Johnson Singh

Occurrence of aphids on various potato germplasms in eastern gangetic plains of West Bengal
...003-04 and 2004-05 at Adisaptagram Block Seed Farm, Hoogly, West Bengal to find out the incidence pattern and population dynamics of aphids on various potato germplasms. The common potato germplasms viz Kufri Chandramukhi, Kufri Jyoti, Kufri Jawhar along with three processing varieties like Kufri Chipsona-1, Kufri Chipsona-2 and Atlantic were screened against aphids. Potato seed tuber was planted by end of November following all standard agronomic practices ex...

sans-serif;">Anil Sehajpal, Saroj Arora and Parminder Kaur 

 

Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...lus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity even at the lowest concentration, i.e. 100 ppm, with relative magnitude of inhibition 2.0 mm against the pathogen R. Solani. With regard to the effect of 8 plant oil samples tested, it was noticed that...

Dhananjoy Mandal

Eco-friendly management of mealybug and wilt in pineapple
...ng planting materials (basal portion) with monocrotophos 36% EC @ 0.02% + phorate 10 G @ 15 kg ha-1at 100 DAP + Neem oil 1500 ppm spray @ 2.5 m/L-1 at 150 DAP]; T3 [Treating planting materials (basal portion) with monocrotophos 36% EC @ 0.02% + phorate 10 G @ 15 kgha-1 at 100 DAP + neem cake @ 1.5 tha-1 at 180 DAP + three times manual weeding]. By yield performance and reduction of percentage of wilted plants and mealy bug p...

Dhananjoy Mandal 2K. Baral 3M. K. Dasgupta

Developing site-specific appropriate precision agriculture
... area but not for the peasant farming or the pest complex ridden unaided agriculture as in India. PA is not about an abstract of characterization. The specific values indicating variations in population at exact grids and helping the individual farmer in decision-making in pest management at varied pesticide doses depending on varied soil pest population intensity and spatial distribution. Often it ends up in reducing the quantum of a pesticide threatened with...

Partha Pratim Ghosh and 2N. C. Mandal 

Some disease management practices for bacterial wilt of potato
...y acidic (pH 5.62) loamy sand soil with medium fertility status in a Randomised Block Design with three replicates and seven treatment modules, as described in Materials & Methods. Investigation revealed that T1 (TPS whole tuber planting) and T4 (supervised management - cowdung @ 40 ha-1 at land preparation + seed piece tuber treatment with carbendazim 2.5 gL-1 and Streptocycline @ 1 gL-1 + stable bleaching powder drenching with out removal of affected pla...

T. P. Ghosh, D. Mandal, S. Laha and M. K. Dasgupta 

Dynamics and severity model in managing fungal diseases
...eaf spot of Ficus religiosa (c.o. Alternaria sp.) is the first record. Fifteen fungal diseases have been formally described from West Bengal for the first time on these twelve ornamentals. Monthly dynamics was determined for occurrence, intensity and severity of these diseases. The diseases (referred as pathogens) that were the highest and fastest during warm and wet (rainy) months were: Alternaria alternata on Polyanthes tuberosa

Shekharappa

Biological control of earhead caterpillar, Helicoverpea armigera Hubner in sorghum
... infective at both the dosages 1x108 conidia per litre & 2x108 conidia per litre against H. armigera as compared to Ha NPV 250 LE per hectare and chemical control in mitigating the pest population as well as in obtaining the higher yield.
 

 

...

Sujoy Mondal and S. S. Ghatak

Bioefficacy of some indigenous plant extracts against epilachna beetle (Henosepilachna vigintioctopunctata Fabr.) infesting cucumber
...extracts of Annona squamosa with methanol as solvent in controlling epilachna beetle, Henosepilachna vigintioctopunctata, Fabr; Both Neem Azal and seed extracts of Annona squamosa were used at 4ml, 5ml and 6ml (per Lt. of water) while this was 1ml, 2ml and 3ml (per Lt. of water) in case of petroleum ether extract of rhizome of Acorus calamus. Endosulfan 35 EC was used at 1ml, 1.5ml and 2ml (per Lt. of water) to compare the e...

Manidipa Chowdhury 

Incidence of saw fly, Athalia lugens proxima Klug. as influenced by level of irrigation and fertilizers on mustard
...o study the incidence of saw fly (Athalia lugens proxima Klug.) as influenced by level of irrigation and fertilizers on mustard c.v. NC-1 (Jhumka) of Brassica campestris var. yellow sarson. Highest saw fly population (0.26 larvae/plant) was recorded on the crop grown without irrigation and medium level of fertilizers (60:30:30 Kg NPK ha-1) while lowest population level (0.10 larvae/plant) ...

Shyam Singh and L.P. Awasthi

Plant products for the management of yellow mosaic disease of mungbean and urdbean
...extracts on the yellow mosaic of mug bean. Maximum reduction in disease incidence recorded were 66.70 and 63.65 percent in mungbean and Urdbean, respectively by eight sprays of Clerodendrum aculeatum. Whereas, eight sprays of Boerhaavia diffusa root extract could reduce the disease incidence by 60.27 and 58.20 per cent followed by Azadirachta indica leaf extract by 42.43 and 42.92 percent in mungbean and urdbean, respectivel...

N. Mandi and A. K. Senapati 

Integration of chemical botanical and microbial insecticides for control of thrips, Scirtothrips dorsalis Hood infesting chilli
... thrips (Scirtothrips dorsalis Hood) infesting chilli (Capsicum frutescens). The effectiveness of four insecticides acetamiprid 0.004% (Dhanpreet – 20% SP), thiamethoxam 0.005% (Avant-25% WG), neem pesticide 0.4% (ultineem 1% W/W) and Bacillus thuringiensis, subsp. kurstaki (BT) at gL-1 was evaluated in the field of Coastal Saline Zone of West Bengal against thrips. It is evident from the result of present investigation that acetamiprid and thiamithoxam ...

S. Pal and I. Sarkar

Pests infesting ornamental plants in hilly region of West Bengal
...gerbera, china rose, chrysanthemum, Bird of Paradise, Straw flower. A number of aphid species were found infesting different ornamental plants viz. Myzus persicae on Carnation, gerbera and Anthurium; Macrosiphoniella sanborni on Chrysanthemum; Aphis gossypii on China rose. Other sucking pests infesting ornamentals included Bemisia tabaci on Gerbera, leafhopper on Gladiolus and scale insect...
Zhensheng Liu1,2, Hui Gao1,2, Jiapeng Dong1, Mingming Zhang3 and Liwei Teng1,2,*
...ivity synchronization of same-sex groups is higher than for mixed-sex groups. Our results support the hypothesis. Both males and females spent most of their time on feeding and moving. Feeding behavior differed significantly by sex but moving behavior did not. Activity synchronization indices of same-sex groups were higher than for mixed-groups and were highest for male groups. The activity budget hypothesis of sexual segreg...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...enic field infectious bursal disease virus (IBDV). The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate a...
Nasreen Memon1,*, Rukhsana Parveen2 and Imtiaz Ahmad2, Nadir Ali Shah3
...Illustrations include dorsal view, scent gland apparatus, external and internal male and female genitalia. A key of nine species is also given.
...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...sin disc diffusion assay. The MIC of lactoferrin was found to be 0.5 mg/mL for E. coli and 0.2 mg/mL for S. aureus.
...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1 
Song Li1,* and Zuojian Feng2
...ated that the allopatric samples from Xizang were obviously different from all those previously defined in Yunnan. Therefore, we describe them as new subspecies P. a. muzongensis subsp. nov. Discussion on the relationships between the differentiation of P. albiventer and its environmental evolution in southwestern China are also provided.
...
Hai-Ji Zhang1, Di Zhang2, Dong-Min Hou1 and Wan-Long Zhu1,*
...re no post-fasting compensatory increases in food intake. All of the results indicated that A. chevrieri can adjust their physiological functions to cope with food shortage, mainly by decreasing body mass, thermogenesis and serum leptin levels.
...
Freeha John, Noor Abid Saeed*, Sajid Nadeem and Muhammad Hamed 
...as conducted at NIAB, Faisalabad to evaluate the use of the planting time and the insecticide sprays for managing aphids to avoid losses to grain yield. During the crop season in year 2015-16, wheat variety (Millat 2011) was sown on three planting dates: mid Nov (crop 1), end Nov (crop 2), and mid Dec (crop 3). Trial was laid out in split plot design having three replications. Planting dates were kept in main plots while, insecticide treatments were kept in su...
Yasemin Bircan Yildirim* and Hediye Tugce Vurmay
...portant finfish species: saddled seabream (Oblada melanura), gilthead sea bream (Sparus aurata), Bogue (Boops boops), horse mackerel (Trachurus mediterraneus), red mullet (Mullus barbatus), brushtooth lizardfish (Saurida undosquamis), grey mullet (Mugil cephalus), threadfin bream, (Nemipterus randalli), which were caught from the Iskenderun Bay at 6 different locations (local fishermen’s fish market...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...selected and offered a basal diet based on sorghum forage and concentrate ration supplemented with fibrolytic enzyme (Fibrozyme™, Alltech Inc. Company, USA) at four levels (T1 = 0, T2 = 10, T3 = 15 and T4 = 20 g/animal/day) in a 4 × 4 Latin Square change-over design with four experimental periods. Each period consisted of a 14-days enzyme supplementation period following a 3-da...
Rauf Ahmad1, Karam Ahad2 and Aziz Ullah Shah3
...t; 40%). Physically, 60% samples were extra-long size, slender shape and coarse type. Nineteen percent samples were long or medium size but slender shape and coarse type as well. Whereas, 21% sample were short or medium size only. Regarding cooking quality, 60% samples were excellent or good and 30% were OK (poor quality). The cooking quality of 10%
Mousa Keshavarz1*, Maryam Soyuf Jahromi2
...26, respectively. Twenty samples by the total wet weights less than 12 g were non-sexual. The wet weight of the gonads was ~8.25% of the mean total wet body weights. The most distribution of gonad wet weights and total wet weights were generally less than 10 g and 80 g, respectively. The females were heavier than the males (~1.77x). The heavier females had more gonads (direct relation, r=0.45) while the lighter males had more gonads (inverse relation, r=-0.52)...
1Sitesh Chatterjee, 1Chirasree Gangopadhyay and 2Santosh Kumar Roy
...arb, flubendiamide, spinosad and chlorantraniliprole to test their efficacy against different rice insect-pests. The study found that whorl maggot can be managed properly in seedbed application with chlorantraniliprole granule 0.4% WW/SR in seven days prior to seedling uprooting along with spraying of indoxacarb 14.5 SC /chlorantraniliprole 18.5% SC /flubendiamide 48% SC in the main-field. The leaf folder was also managed properly by application of chlorantran...
P.P. Ghosh*, A. Chakraborty, S.K. Bhattacharya, C. Ghosh, B. Mahato and M.K. Bhattacharjya
...the present findings for safe storage of black gram for the purpose of food and seed under homestead conditions of Purulia district of West Bengal.
...
Mohammed M. Ibrahim1,2*, Y.S. Chandel1 and Anil3
...pis mellifera L. and samples were collected from four locations representing two different agro-climatic zones of Himachal Pradesh during 2012. The findings revealed that the significant differences were observed between the populations for all characters except abdomen length, fore wing width and length of trochanter and tibia. Overall, the mean of populations for head height × width, proboscis length, thorax length, abdomen length, fore wing length...
Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...achol, copper and silver salts along with their synthesized nanoparticles were evaluated for their antibacterial study against Staphylococcus. aureas, Escherichia. coli and Bacillus. subtilis. The results obtained for their comparative evaluation revealed that silver-Lapachol and copper-Lapachol nanoparticles have greater antibacterial efficacy against all three aforementioned strains of bacteria at 5 µg/ml, 40 µg/ml and 80 µg/ml concentratio...

Gadeeyya G and Ratna Kumar P.K.

...undus, Crotalaria verrucosa, Digera muricata, Sida cordifolia, Ipomoea pestigridis and Trianthema portulacastrum were selected for in vitro studies. Fungal isolates namely Ascochyta cypericola, Bipolaris sp., Chaetomium globosum, Colletotrichum capsici, Colletotrichum sp., Curvularia lunata, Curvularia tuberculata, Fusarium oxysporum, Helminthosporium sp. and Gibbago trianthemae ...

Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2

...om 5.1 to 0.4 log2 and disappeared with in a month. While MDA for NDV remained 2.1 log2 until 28th day of age, the Geometric mean titers (GMT) for H5 were increased from the 7th day of age using both antigens (RE-5 antigen) and (S75/Egy/2015 antigen). Generally, GMT post-challenge was increased as the chicks overcome the infections. In conclusion, our results indicate that vaccination at 5th day old with baculovirus H5+ND inactivated vaccine can interfere with...
 

Amir Ismail1, Muhammad Riaz1,2*, Saeed Akhtar1, Amjad Farooq3, Muhammad Arif Shahzad1 and Ahmad Mujtaba4

 

...ere analyzed in the milk samples of cow, buffalo and goat. The level of Pb was found to exceed the maximum permissible limit (0.02 µg/g) set by Codex Alimentarius Commission in 53% milk samples. In 44% milk samples the level of Cd was found to exceed the permissible limit (0.0026 µg/g) set by International Dairy Federation (1979). The mean levels of Ni, Cu and Co were found in ...
Süleyman Kök*
...ts being negative in the sample populations for CAST loci imply heterogeneity in CAST loci (P<0.001). The effective allele number and allele density are other criteria to show the spatial heterogeneity in a population. The average effective allele number (ne) per loci for TGC samples from the smallholders in the villages, TGC ex-situ conservation herd and the total population sa<...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...t genes derived from the same GST class from different species share more similarity than genes of different classes in the same species. Copy number of GSTs examining showed that five classes of GST genes in common carp have undergone the gene duplications, including MGST1, GSTK, GSTM, GSTA and GSTT. Comparative genomics and syntenic analysis provided new evidences for better understanding on gene fates post whole genome du...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8Naimat Ullah Malik1 and Abdul Razzaq9
...r this purpose 360 stool samples were collected from the District Headquarter Hospitals of all the three districts and were screened through microscopy. Results yielded an overall prevalence of 11.11%, with significant difference (P<0.05) among different study areas. Prevalence was highest in district Bannu (11.66%), followed by district Lakki Marwat and Kohat with 10% in each district. Statistical analysis revealed significant difference (P<0.05) among ...
Mutassim M. Abdelrahman1,*, Riyadh S. Aljumaah1, Moez Ayadi1 and Shabana Naz2
...n) and iodine (I). Blood samples were collected from ewes and their newborns at parturition. Colostrum yield and biochemical analysis at parturition was also determined. A significant (P<0.05) increase of calcium (Ca), Zn, Co and Se levels in blood of ewes treated with bolus with a significant increase in phosphorous (P), Co and Se in the blood of their newborn lambs. Furthermore, a significantly higher (P<0.05) inorganic matter percentage in colostrum w...
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
...tan. A total of 60 blood samples were collected from peafowl (30 males and 30 female) in months of March and April, 2015. The gender difference showed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the serum total protein and Mg concentrations were significantly lower (P < 0.05) in male compared to female peafowl. In conclusion, the present study provides base-line values for hematology and serum biochemistry of apparently ...
Muzammil Sattar
...: Casein, Protein hydrolysate, Torula yeast and Nu lure. Al these proteins were tested in laboratory at four concentrations, i.e., 1, 3, 5 and 7 mg/ml in diet. Significantly higher fecundity 785.12±25.7, fertility 89.23±0.36 % of eggs and longer oviposition period 42.13±1.11 days were recorded in adults feeding on a diet containing Nu lure followed by protein hydrolysate (control diet). The F...
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...orbid material and fecal samples from 600 different locations across the region were analyzed for isolation. Isolation was carried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase polymerase chain reaction (RT-PCR) procedure. Out of 600 examined locations twenty two (22) H9N2 subtypes isolates were recorded. The to...
Nasar Khan1,2, Fahad Ali3,7, Sulaiman Bahadar5,*, Khalil ur Rahman3, Abdul Aziz3, Noor ul Akbar4, Muhammad Daud6, Azam Hayat5 and Mujaddad ur Rehman5
...79.21% of HBsAg positive samples, showing that HBsAg on ELISA is much more sensitive than PCR as HBV shows chronic conditions and both of the tests are needed against HBV.
...
Mutassim M. Abdelrahman1*,Ibrahim Alhidary1, Abdullah H. Alyemni2, Rifat U. Khan3, Abdel Raouf S. Bello4, Mohamed Y. Al-Saiady2 and Ramzi A. Amran1
...e days (T4). Rumen fluid samples were analyzed for volatile fatty acids and ammonia nitrogen levels. Acetic acid improved significantly in experimental groups, while propionic acid decreased significantly in T1. Valeric acid and butyric acid decreased significantly in T1 and T2 while isovolaric acid and the ratio of acetic acid and propionic acid and glucose increased significantly in T1. In conclusion, feeding TMR with alfalfa hay, regardless of the supplemen...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...) were selected from the same ancestry during August 2011 to January 2014fromSir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore. The demographic values for age, employment and marital status did not differ significantly among case and control groups. Exonic regions of BDNF gene were sequenced. In-silico analysis was performed to detect the possible disease causing effect of genetic variants with OCD. Sequencing of BDNF gene show...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...al or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identified polymorphism, RNA secondary structure analysis was performed and SNP was found changing the termination loop shape in mRNA. However, to increase the selection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backgrounds.
...
Muzafar Shah1,*, Mian Sayed Khan1, Muhammad Ather Rafi2, Habib Ahmed3 and James M. Carpenter4
... justify;">A total of 67 samples of Ropalida brevita were collected from District Abbottabad, Pakistan during 2011 to 2013. Species was confirmed by taxonomic key, which are belonging to subfamily of Polistinae. Phylogenetic tree were reconstructed using 12 SrRNA (12S) gene showing homology of 73% compared with gene bank data while on the basis of cytochrome oxidase 1 (Co1) gene 93% homology were recorded. It was concluded that Co1 gene is...

Umer Wahid1, Shahid Ali2* and Nihal Ahmad Hadi3

...Pakhtunkhwa, Pakistan. A sample of 120 tomato growers was selected employing multistage sampling technique. An interview schedule was used for data collection during November-January, 2016. Maximum likelihood technique was used for the estimation of the Cobb-Douglas stochastic frontier production model. After running various econometrics software the output of the Frontier 4.1 was found good fit. The findings of the study sh...

Naveed Muhammad, Syed Attaullah Shah*, Abbas Ullah Jan, Irfan Ullah, Muhammad Ibrahim and Salman Khan

...ned questionnaire from a sample of 115 tomato growers. A Cobb-Douglas type production model is used to estimate tomato yield and identify important factors affecting yield. Ordinary Least Square estimates for the model indicate that machinery, seed quantity, labor, number of irrigation, chemicals spray and fertilizers have positive significant effects on tomato yield. Allocative efficiency analysis reveals that machinery, seed quantity, number of irrigation, f...
Muhammad Mansoor1, Amanullah1, Zafar Islam2*,Sher Muhammad3, Muhammad Umair4, Mehmood Ayaz5, Asghar Ali Khan6, Muhammad Asif2 and Ya Sakina3
...ble to Mungbean Yellow Mosac Virus (MYMV) and NM-92 (a promising variety of Pakistan, bold seeded, high yielding and MYMV resistant) at Agricultural Research Institute, Dera Ismail Khan. Inqalab Mung was tested in various experiments for production technology and yield trials for its performance. Result showed that 40 kg ha-1 seed rate with 30 cm row spacing, fertilizer dose @ 20:50 kg ha-1 N:P2O5 and inoculatio...

Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3

...lal Method and the blood samples of the birds were preserved for antioxidant enzymes assay analysis. The data showed that as the citrus waste percentage in the feed of the broiler was increased, its feed intake and the body weight decreased but the citrus waste has non-significant effect on the feed conversion ratio of the broiler birds. The results also showed that citrus waste in the feed have positive effect on the antiox...
Oaj Khurshid1*, Naureen Aurangzeb1, Ayaz Khan1, Alia Naz1, Abdullah khan1, Sobia Nisa2 and Sehrish Sajawal3
...eat proportion of Decomposable organic matter, adequate moisture content and C/N ratio. On windrow composting, not only the bulk of the waste was compacted but also formed a powdery earthy stinking soil-like, compost material. All valued factors in the compost samples were found to be within the acceptable limits set by international standard. The pH fluctuated between 7.5-8.1, moisture 35% and organic matter 43%, and has ad...

Shah Saud Ahmad and Muhammad Zafarullah Khan*

...udy was conducted in Charsadda district in the year 2016 to investigate the role of Farm Services Center (FSC) regarding empowerment of vegetable growers. Primary data were collected from 234 vegetable growers through well-structured interview schedule. Whereas secondary data were amassed from various published and unpublished sources. Results of the present study revealed that majority (85%) respondents had joint families system. Almost (60%) respondents had ...
Ayesha Ilyas1, Hafiz Azhar Ali Khan2,* and Abdul Qadir1,*
...vetia peruviana) and safeda (Eucalyptus camaldulensis) against the peach fruit fly, Bactrocera zonata (Saunders), at 2% concentration in a free choice bioassays. Acetone, chloroform, petroleum ether and ethanol were used for extraction from leaves. Amongst the various treatments applied, the acetone extract of D. alba showed the highest repellency of 84.14%, whereas, the lowest repellency of 10.73% w...
Hazrat Ali1,*, Wajid Ali1, Karim Ullah1, Fazle Akbar1, Sher Ahrar1, Irfan Ullah1, Irum Ahmad1, Ashfaq Ahmad1, Ikram Ilahi2 and Muhammad Anwar Sajad3 
... were determined in fish samples by atomic absorption spectrophotometer. Highest Cu concentration of 4.52 ± 0.24 mg kg−1 wet weight was observed in muscles of M. armatus at Chakdara on River Swat while highest Zn concentration of 18.00 ± 1.88 mg kg−1 wet weight was observed in muscles of S. plagiostomus at Daggar on River Barandu. A site wise comparison of both Cu and Zn concentrations in muscles of...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...tized individuals. Blood samples of aeroallergen sensitized individuals were collected at the Allergy Centre, NIH, Islamabad. Then samples were analysed at the Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore during the study period expanded from August 2014 to February 2015 for the presence of HLA-DRB1*11 and HLA-DRB1*12 allele using polymerase chain reaction (PCR). While tot...
Mazher Abbas1, Irfan Mahmood1*, Arshed Bashir1, Tahir Mehmood2, Khalid Mahmood1 and Zubair Ikram1
...ces of citrus. The study sample was consisted of 140 respondents. The binary regression model has been used to evaluate the effect of different socio-economic variables on the adoption response of different recommended production practice for citrus. Average age, education and experience of the citrus growers were estimated 43.92 years, 9.74 schooling years and 17.72 years respectively. The awareness and adoption of recommended citrus practices was generally g...
Hassen Allaya1,2,*, Abderraouf Ben Faleh1, Manel Rebaya1, Sonia Zrelli3, Abdallah Hattour2, Jean-Pierre Quignard4 and Monia Trabelsi1
...tus from two distant sampling sites (Mahdia and Zarzis) along the Tunisian coast using 11 morphometric and 3 meristic characters in order to investigate the morpholgical variation among them. Uni and Multivariate analysis of variance of 64 adult specimens showed significant differences among the means of the two studied sites for 8 standardized morphometric measurements out of 11 characters. The principal component analysis (PCA), scatter plot of individua...

Muhammad Zafarullah Khan

... that is considered necessary in order to deal with farmers where a wide range of variations exists in their personalities and habits. A significant difference was reported in required and possessed competencies of preparing training schedules regarding crops followed by timely appointment and issuance of duties to junior staff for effective and efficient work. Results of the study reported that there is utmost need of training in competencies regarding identi...
Adnan Yousaf1,Jia Wu1, Qaiser Shakeel2, Yasir Iftikhar3, Muhammad Irfan Ullah4, Uzma Tahira5,Mustansar Mubeen1 and Wubei Dong1,*
...nt by using derosol, cadusafos and Trichoderma harzianum in the green house at 25 ± 4ºC. Primarily, 15 chilies cultivars viz., 28-2010, C-72, Sanam, C-73, Gola Peshawari, 27-2010, Tata Puri, C-302, C-33, C-19, 5-2010, C-68, 11-2010, 18-2010 and 15-2010 were evaluated against M. incognita and S. rolfsii. Two week old chilies plants were inoculated with M. incognita, S. rolfsii separately and in combination a...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...and immunofluorescence assays. The humoral immune response was evaluated by intramuscular immunization of specific pathogen free (SPF) chickens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 1...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and  Kui Chen2,*
...iver, and belongs to the same genus with Red fin puffer T. rubripes which has been used as one of human genome model animal since mid-1990s. In this experiment, we cloned a pair of DNA fragment sequence including complete ORF (921bp) of taste receptor candidate gene T2R1 of T. fasciatus and studied the differential expression spectrum of tissues in combination with bioinformatics analysis so as to understand the possible functions in respo...

Murad Ali1, Farmanullah Khan1, Wiqar Ahmad2* and Imran Khan1

...organic sources is universally accepted agronomic practice for improved yield and soil properties. However, determination of a suitable dose from either source that could beneficially and easily combine to give maximum output still needs lots of research. The experiment was conducted during wheat season 2014-15 on a selected water eroded site in District Swabi. Fourteen treatments viz the control (T1), recommended dose (RD) (120:90:60 kg ha-1 N: P2O5: K2O. T2)...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
Rahat Naseer1*, Abu Saeed Hashmi1, Zulfiqar-ul-Hassan2, H. Rehman1, Saima Naveed1, F. Masood1 and M. Tayyab1
... present project was envisaged by using rice husk as a substrate for processing with the objective to produce a nutritionally enriched bulk feed ingredient and to explore its potential as wheat bran replacer in total mixed rations for the ruminants. For this purpose rice husk was subjected to different processing techniques using acid, alkali, water treatment and fermentation to prepare processed moiety on pilot scale. All these processed materials were includ...

Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4

...lated from 400 suspected samples and were confirmed through serological and molecular assays. The sequence analysis of NA genes shown 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all the isolates belonged to the G- lineage. Amino acid analysis of NA stalk regions of these isolates illustrated no insertion, deletion and substitutions compared to A/DK/HK/Y280/97 l...

Attaullah Khan1, Muhammad Shafi1*, Jehan Bakht2 and Shazma Anwar1

...ntitled “Effect of salinity and seed priming on growth characters of Wheat varieties” was carried out at Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar during winter 2013-2014. The experiment was conducted in completely randomized design (CRD) with three replications. The response of fourteen wheat varieties at two seed treatments (primed with 50 mM CaCl2 and un-primed) with four

Sania Shaheen1*, Hina Fatima2 and Muhammad Azeem Khan3

... Data collected from 300 sample rice farmers into the Kharif cycle (2013-14) at five main rice growing districts of Punjab namely: Hafizabad, Sheikhupura, Jhang, Vehari, and Gujranwala. Stochastic frontier analysis (SFA) was applied to find the results of a study. Study results reveal that direct seeding method is more profitable for dry rice farmers in terms of yield and also increases the efficiency of farmers. Area under rice crop, NPK ratio, Seed, number o...
Rais Ahmed1,*, Khushi Muhammad1, Masood Rabbani1 and Muhammad Sarwar Khan2
...jab including Lahore, Faisalabad, Sheikhupura, Sargodha, D.G. Khan, Chakwal, Sahiwal, Gujranwala and Attock were determined. By using grid based sampling strategy, soil samples (n= 1280) were collected from villages (n=256, 5 samples/village) of the nine districts. Brucella genus specific DNA (bcsp31) was detected using Real Time PCR and pr...
Kanwer Shahzad Ahmed1, Muhammad Zeeshan Majeed1,*, Muhammad Ather Rafi2, Fatima Sellami3 and Muhammad Afzal1
...icraspis allardi (Mulsant 1853) and Propylea dissecta (Mulsant 1850) belonged to tribe Coccinellini (Latreille 1807) of subfamily Coccinellinae (Latreille 1807). Two species belonged to tribe Chilocorini (Costa 1849) of subfamily Chilocorinae (Mulsant 1846) i.e. Brumoides suturalis (Fabricius 1798) and Exochomus nigripennis (Erichson 1843). Two species namel...
Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4
...enofos, endosulfan, spinosad and deltamethrin) in multi-floral raw honey of Apis dorsata F. collected from the cotton belt area of Punjab, Pakistan. The residues of these insecticides were extracted using ethyl acetate. Honey samples were spiked at 0.1 and 0.01 mg/kg. The mean recoveries of these insecticides in the spiked samples were 74-92% with...

Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3

...mucopurulent to bloody nasal/ocular discharges, watery to bloody diarrhea and lameness. The major gross lesions were erosive stomatitis, hemorrhages in lung, liver, abomasum, small and large intestine. PPRV NP was detected in sheep (n=20) and goats (n=18), anti-PPRV NP was also detected among sheep and goats (n=162) by ELISA, 3ABC-FMD enzyme immunoassay ...

Wali Khan1, Naushad Khan2 and Shaista Naz2*

... this, through purposive sampling technique a sample size of 80 bee keepers was selected from the three tehsils of the agency (i.e; Khar, Salarzai, and Nawagai). The data were collected from the sampled bee keepers using a pretested questionnaire by face to face interview in 2014 production period. The collected data were analyzed using frequencies, percentages, descriptive statistics, ran...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...fectively and relatively safer for natural enemies and beneficial arthropods. Present investigations were carried out to determine their impact on larval mortality, pupal and adult emergence of 2nd and 4th instar larvae of Spodoptera litura F. under laboratory conditions. Both NPV and flubendiamide were applied using diet incorporation method. Larvae were allowed to feed for 48 h. Mortality was recorded until larvae died or pupated...
Talha Nazir1,2,*, Muhammad Dildar Gogi2, Muhammad Zeeshan Majeed1, Waheed ul Hassan2, Abdul Hanan2 and Muhammad Jalal Arif
... proved to be relatively safe. All insecticide formulations tested exhibited ≥ 50% reduction in the population of green lacewings. Similarly, whitefly parasitism recorded in the Confidor and Jozer plots was very close to that of control plots for both post-treatment observations. Based on these findings, Confidor and Jozer are recommended to be considered for their integration in sucking pests’ management strategies on transgenic crops such as Bt cott...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
...amily to find out the causative mutation. All coding exons of CTSK gene were amplified and sequenced in the affected and unaffected individuals of a consanguineous Pakistani family consisting of five members, three affected brothers, one unaffected sister and the unaffected mother. We identified a known missense mutation c.136 C>T in the third exon of CTSK changing arginine to tryptophan (p.Arg46Trp) which s...
Ayşe Gül Harlıoğlu*
... eggs, eyed embryo, yolk-sac larvae and absorbed yolk-sac larvae were investigated in rainbow trout(O. mykiss). The polyunsaturated fatty acids (PUFA) were found the highest followed by monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in the unfertilized eggs. On the other hand, PUFA was the hi...

Muhammad Ibrahim1,2*, Syed Jawad Ahmad Shah1, Shaukat Hussain2, Musharaf Ahmad2 and Farhatullah3

 

...d 1600 wheat fields were sampled covering a geographical area of little less than 5000 acres in the southern, central and northern zones located in the Khyber Pakhtunkhwa (KPK) Province. Yellow rust occurrence was distributed over an area of 463 acres in all three wheat production zones. Maximum yellow rust impact values (estimated by Field Impact Factor (FIF) of 121, 201 and 328 were recorded in Bannu (Southeren Zone), Nowshara (Central Zone) and Mansehra (No...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...roots as well as in soil samples (81.7%) collected from infected fields. Among nematodes, Meloidogyne incognita (Kofoid and White) chitwood showed the maximum frequency percentage (72.3%). Results confirmed the presence of fungi and nematodes interacting each other to enhance the disease. Paecilomyces lilacinus (Thom) and Trichoderma harzianum (Rifai) (alone and in combination) were used as bio-control agents against isolated pathogens at ...

Raza Ullah1*, Qaisar Shah Safi2, Ghaffar Ali3 and Irfan Ullah

...ected and a total of 120 sampled respondents are selected using proportional allocation method. The findings revealed that majority of the farmers sold their orchards to the pre-harvest contractors. The most widely used marketing channel was producers selling their orchard to pre-harvest contractor who then harvest the orchard, pack the produce and transport it to the market where the produce is handed to wholesalers. Whole<...
Usman Waheed1,*, Humayoon Shafique Satti2, Muhammad Arshad3, Ahmed Farooq3, Abdul Rauf4 and Hasan Abbas Zaheer1,5
...ry 2016, community-based sampling was done from 134 Hijra sex workers (HSW), 101 female sex workers (FSW), 104 jail prisoners and 48 intravenous drug users (IDUs) through community outreach, peer referring and field sampling. Blood samples were tested for syphilis and HIV infections after pre-test counseling and receiving informed written consent. Initial testing was performed by rapid dev...
Kadir Karakuş1, Bahat Comba2, Abuzer Taş3, Tunahan Sancak3, Arzu Comba2, Devrim Saripinar Aksu4, Hasan Koyun5 and Mohammad Masood Tariq6,*
...up), respectively. Blood sample was taken from vena jugularis of the animals before the application and every two months for a total of 4 times for 6 months and hormone analyses were performed. The order of statistical significance for TSH (thyroid stimulating hormone) were found as Surgical > Control > Formol (P <0.05). The order of statistical significance for growth hormone was Formol = Surgery > Control (P<0.05). In August month, the applica...
Ibrahim A. Alhidary1,*, Mutassim M. Abdelrahman1, Riyadh S. Aljumaah1, Abdullah H. Alyemni2, Moez A. Ayadi1,3 and Mohamed Y. Al-Saiady2
...rded weekly. Rumen fluid samples were collected monthly after 2, 4 and 8 hours of feeding for pH, VFAs and NH3-N determination. Blood samples were collected monthly for different metabolic analysis. At the end of the trial, six lambs from each group were randomly selected and slaughtered for rumen coloring. The results of this trial showed a significantly higher total body weight gain in lambs fed TMR1 and TMR 2. ...
Muhammad Abdullah1,*, Rashid A. khan2, Muhammad Rafay3, Tanveer Hussain3, Tahira Ruby4,Fariha Rehman5, Sangam Khalil3 and Sohail Akhtar6
...e surrounding area of Faisalabad. Based on results it was concluded that Cattle egrets are colonial breeders and colonies were monospecific with no other ardeidae members nesting in the neighborhood. They prefer sites for nest building, which have regular accessibility of water, and usual human activities are common nearby. The breeding period was discerned to be start from April to August and Acacia nilotica was observed as most preferred tree for nest...
Tanveer Ahmad1, Hafiz Sultan Mahmood1*, Zulfiqar Ali1, Muhammad Azeem Khan2 and Shahid Zia3
... enormous potential of sisal fibre is present in Pakistan for making ropes, twines, paper, carpets, cloth and handicrafts, which has not been exploited so far. The aim of this study was to design and develop a small-scale portable sisal decorticator for extracting fibre from sisal leaves. A prototype of sisal decorticator was designed and fabricated at A...
Muhammad Tariq Saeed1*, Muhammad Ashfaq Wahid1, Muhammad Farrukh Saleem1, Mumtaz Akhtar Cheema2, Muhammad Shahid1, Abdul-Shakoor1, Abdul-Sattar3
...ersity of Agriculture Faisalabad, during spring 2011. The experiment was composed of two soybean varieties (Faisal Soybean and 95-1) and five priming techniques (control, on-farm priming, hydro priming, hydration inoculation and osmo priming with 0.5% urea solution. Results revealed that hydro priming, on-farm priming and hydration inoculation significantly reduced the time to 50% emergence (E50), mean emergence t...
Shazia Rasheed1,* and Javed Mustaquim2
... pelagicus and p. sanguinolentus, they belong to five species; namely Octolasmis angulata (Aurivillius, 1894), O. cor (Aurivillius, 1894), O. lowei (Darwin, 1851), O. tridens (Aurivillius, 1894) and O. warwickii Gray, 1825. The rate of infestation was found markedly low in P. pelagicus (2.9 %) and P. sanguinolentus (10.8 %) during present investigation. Octol...
Omar S.O. Amer1,3,*, Mohamed I. Waly2,4 andSaeed A. Al-Zahrani1
...28, 0.25%) and Taenia saginata (n= 21, 0.19%) respectively. The prevalence rate of these parasites in males and females as well as different age groups per month / year is provided. Intestinal parasitic infection is still a public health problem in Riyadh region, KSA. It is necessary to update the epidemiologic survey of the parasitic infection at...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
... species exhibiting a dorsal keel have been identified based on morphology: Liza affinis, Liza carinata, and Liza klunzingeri. To confirm the validity of the species by molecular methods, we sequenced a fragment of the cytochrome oxidase subunit I (COI) gene of mitochondrial DNA of Liza affinis and Liza klunzingeri from coastal waters of China and Pakistan, respectively. Sequences of L. carinata were obtained from GenB...

Jawad Anwar* and Zafar Iqbal 

...oli, Pseudomonas aeruginosa and Staphylococcus aureus) using 96 well microtitre plate method. The Ethyl acetate fraction showed maximum zone of inhibition (24.25±1.06 mm) against X. campestris and (23.15±2.12 mm) against C. michiganensis on growth nutrient broth (GNB) medium as compared to other culture media (Potato dextrose broth and yeast extract broth). Similarly maximum zone of inhibition was observed in optimized parameters like shaking fla...

Muhammad Amin1*, Khalid Mahmood2, Imran Bodlah3, Muhammad Rahim Khan2 

..., were found infesting Rosa species in study conducted during 2015-2016 in Poonch division of Azad Jammu and Kashmir-Pakistan. Chaetosiphon (Pentarichopus) fragaefolii (Cockerell), Chaetosiphon (Pentarichopus) thomasi Hille Ris Lambers, Chaetosiphon (Pentarichopus) tetrarhodum (Walker), Metopolophium montanum Hille Ris Lambers, Myzaphis rosarum (Kaltenbach) and Myzaphis turanica Nevsky are new records for the country. New lo...

Muhammad Zafarullah Khan*, Shah Saud Ahmad and Asif Nawaz 

...s (FSCs) in district Charsadda regarding provision of agricultural services to its member farmers. About 57% respondents were registered with FSC since last 4-6 years period. Also 57% of the respondents mentioned that the extension agent was the source of awareness about FSC. About 43% of the respondents were invited by FSC for general body meeting whereas only 26% participated in the process of election. Maximum respondents were provided farm machinery by FSC...
Mohammad Umar Farooq1*, Sarfaraz Ahmad2, Ghulam Nabi1, Ijaz Ali1 and Imtiaz Ahmad1
...mental plot. Twenty four samples of each experimental plot were collected with the help of ADC one m 2 quadrate methods, weighed and then oven dried at 60 oC to find out the dry weight for above pytomass carbon Mg C ha-1 (Brown and Lugo, 1982). Similarly, the same 1 m2 quadrate area was dug up to the depth of root zone for each grass and the root portion was separated, weighed, ove...
Saleem Khan1, Amir Hamza2, Farhadullah Khan3, M. Subhan4, Aziz Khan1, Irfan Ali Shah1, Shakirullah Khan Shakir3*
...>Co. The irradiated seed samples were assumed treated seeds while non-irradiated seeds of each genotype were used as control. Field experiment was conducted in randomized complete block design (RCBD) in three replicates in the green house. Radiation effects were observed on some agronomic traits like Plant height, Number of branches plant-1, number of bolls plant-1, Fiber Fineness and yield of seed. The analysis of variance (ANOVA) reveal...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,
...>...
Gülnur Metin* and İlker Aydin
...1.6 g, respectively. The samples were composed of 56.6% females and 46.6% males, with a female to male ratio of 1:0.77. The spawning period was determined from May to October. The total lengths at first maturity of females and males were 10.64 cm and 10.16 cm, respectively.
...
Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
....39% (between Egretta sacra and Egretta garzetta) to 41.15% (between Egretta sacra and Botaurus stellaris). Five conserved sequence boxes (F to B) in the domain II of Ardeidae sequences were localized. Maximum likelihood method was used to reconstruct the phylogenetic trees based on HKY+ G model. The phylogenetic tree grouped members of every genus were grouped into each clade, except the genus
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...January 2014. During the sample collection the factors like age/period, employment status and marital status were considered after informed consent. We found one single base change G>C at c.745 resulting in a nonsynonymous change p.E249Q in theCOMT gene. In-silico analysis predicted it to be damaging and disease causing. Screening of case and control group data showed no deviation from Hardy-Weinberg equilibrium with p-value 0.879 and 0.32, respectiv...
Muhammad Fiaz1, Khadija Munawar2, Muhammad Mushtaq2,*, Mukhtar Ahmad3, Mushtaq Ahmad Chaudhry3, Muhammad Aslam3, Tanveer Ahmad1 and Nasrullah4
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...h acridine orange (AO) assay is easy and less costly. Though used in many species but the variability exists in technique. Therefore, the present study was aimed to optimize the steps of AO staining protocol for buffalo sperm which has not been explored yet. Semen was collected from two mature buffalo bulls, diluted in tris citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained usi...
Shabana1,*, Farah Ehsan1 and Shahida Hasnain1,2
...on. A total of 900 blood samples (diabetic=451, controls=449) were genotyped by PCR-RFLP. A significant difference in genotype distribution was observed between cases (Gly/Gly, 66.9%; Gly/Ser, 20.17%; Ser/Ser, 12.8%) and controls (Gly/Gly, 67.4%; Gly/Ser, 28.5%; Ser/Ser, 4.00%) (p = 0.013). The cases showed significantly higher frequency of the Ser allele compared to the controls (22.9% vs. 18.3%; OR: 1.32, CI: 0.95–1.83, p=0.013). The mino...

Abdur Rab1*, Muhammad Sajid1, Naveed Ahmad1, Khalid Nawab2, Syed Ghias Ali3

... on tomato crop grown in saline conditions was investigated by exposing tomato plants to 0, 75 and 150 mM salinity; and foliar application of 0.0, 0.25, 0.50, 0.75, 1.0, 1.25% calcium solutions. Salinity stress increased leaf Na+ and Na+/ K+, fruit firmness and blossom end rot (BER) incidence but significantly decreased the leaf K+ and Ca content of the fruit and yield. The foliar calcium application decreased the Na+ accumu...
Umair Talib1*, Ijaz Ashraf1, Khalid Mahmood Chaudhary1, Riaz Ahmad2
...er rice growers are more satisfied with public or private extension organizations and development of a strategy to enhance the effectiveness of extension work. The specific objectives were to ascertain the satisfaction smallholder rice growers regarding the extension work conducted by public and private extension organizations; to analyze the satisfaction of smallholder rice growers w...

Qudsia Khalid, Safdar Hussain Shah*, Faiza Zaeem and Saad Hussain Shah 

...t studies calli of Oryza sativa. L. cv. Swat-1 were incrementally adapted to ionic stress (25 mM LiCl) to asses some of the adaptation mechanism(s). Adapted calli line was obtained on MS medium supplemented with 2,4-D (2 mg/l) and kinetin (0.25 mg/l). For regeneration MS medium with 1.0mg/l NAA and BAP + 30g/l D-Sorbitol was used. LiCl adapted line showed significantly higher indices of tolerance than unadapted line at 100, 150, 200 and 250 mM NaCl stress. Ada...

Mian Ahmad Raza*, Ghulam Hassan and Naqib Ullah Khan 

... Fakhr-i-Sarhad (FS), Pirsabak 2005 (PS), AUP-5008 (AUP), Saleem 2000 (S2K), Tatara (TTR), Barsat (BST) and Siren (SRN) were crossed in all possible combinations during 2011-12 cropping season. The resultant F1 hybrids were planted following a randomized complete block design using three replications in the subsequent wheat growing season of 2012-13. For days to maturity, F1 cross combination PS × FS among the F1 hybri...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

...hich LET caused sex reversal, juvenile carp (120dph) were exposed to 0, 5, 25, 125, and 625μg/L LET for 15 days, and the expression profiles of seven gonad differentiation related genes (Cyp19A, Cyp19B, Arα, ERα, Dax, Foxl2, Dmrt1) and gonad histological changes were examined. The results showed that LET could inhibit oocyte growth in female and promote the development of testes in males. For the gonad differentiation related genes, the expressi...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...ii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectively. The blaNDM-1genewas amplified by PCR and sequenced. MIC of different antibiotics were determined by VITEK 2 system and antibacterial activity, MIC and MBC of manuka honey was performed by...
Muhammad Abu Bakar1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Muhammad Irfan Ullah1,*, Muhammad Arshad1, Mubasshir Sohail1,2 and Jaime Molina-Ochoa3,4
... all essential oils gave satisfactory control of Varroa mites except orange oil. The percent mortality was observed higher in eucalyptus oil (76.13%) at 2.5ml dose. Similarly, at 5ml dose, eucalyptus oil (90.27%), lemon oil (85.54%) and neem oil (82.69%) gave significant control of Varroa mites. With the passage of time, mite mortality percentage increases. Overall, eucalyptus oil at 5ml dose rate gave best res...

Mohsan Ullah Goraya1,2, Liaqat Ali1,2 and Iqra Younis3  

...align: justify;">The mucosal surfaces of respiratory, intestinal and reproductive tracts are the primary entry points for viruses into host cells. Amongst these, respiratory tract is prone to direct and continuous exposure to viral infection. Respiratory viruses including influenza virus, coronaviruses and herpes viruses are important pathogens that cause significant losses in the poultry industry. However, successful establishment of infection in the respirat...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...s followed by Heamaphysalis, Hyalomma, Dermacenter and Amblyomma with the prevalence of 78.50%, 10.33%, 10.08%, 0.67% and 0.42%, respectively. On species basis, R. (Boophilus) annulatus, R. (Boophilus) microplus, Heamaphysalis aciculifer, R. appendiculatus, and R. decoloratus were noted 41.67, 18.42, 9.83, 8.25, and 6.83% respectively, whereas least prevalence was noted as 0.42% in c...
Jie Wu1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yingying Liu1, Yanzhen Bu1,* and Hongxing Niu1,*
...ndangered bird. Aimed to save and restore the small population in Henan Province, China, a reintroduction program was conducted in the Dongzhai National Nature Reserve in 2007. Wild birds bred in semi-wilderness were reintroduced. Habitat selection and breeding ecology of crested ibis in Dongzhai National Nature Reserve has not been studied. To clarify the breeding status of the crested ibis and to provide more reference for the reintroduction of this species,...
Nargis Bano and Khalid Mahmood Qureshi*
...e-harvest application of salicylic acid and drought treatments. The design of the experiment was a factorial randomized complete block design (RCBD) with five water regimes (150ml, 175ml, 200ml, 225ml, 250ml) and five levels of SA at (0.0mML-1, 0.5mML-1 1mML-1, 2mML-1, and 3mML-1) as the main factors and four replications. The experime...

Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob

...urpose, liver and spleen samples (n=90) were collected from day-old-chicks derived from breeders at 14, 21 and 30 days of post-infection with AAV-4. The presence of AAV-4 DNA was detected through PCR. Chicks from virus-challenged breeders failed to show a clear and expected PCR positivity. The connotations derived from these findings are that vertical transmission via transovarian route is reported the field conditions. However, based on genetic detection such...

Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1 

 

...ccine A contains IBV (Massachusetts M41 and D274 strains), NDV (Clone 30), aMPV (BUT 8544) and IBDV (classic strain) whereas vaccine B contains IBV (Massachusetts M41 and a Brazil type strain), NDV (LaSota strain), aMPV (TRT strain) and IBDV (GP82 strain). Viscosity values for vaccines A and B where determined to be 40.2cP and 83.3cP, respectively, and a microscopic examination showed that vaccine A has a homogeneous emulsio...
Aqsa Mehboob1, Noor Khan1,*, Usman Atiq1, Khalid Javed Iqbal2, Rafia Tayyab1, Syeda Suhaira Batool1, Hafiza Saleha Batool1, Sana Amjad1 and Mehwish Tanveer1 
... the parameters remained same. Apparent digestibility of crude protein and fat was found non-significantly different among treatments. It is concluded that addition of fenugreek in fish feed can improve growth performance and also protein content of the fish meat as well.
...
Nasira Kazi1, Muhammad Israr2 and Shahina Fayyaz1,*
...l body diameter long, dorsally curved; prerectum 1.7-2.6 anal body diameter long.Plectus (Ceratoplectus) armatus (Butschli, 1873) Andrassy, 1984 is recorded for the first time in Pakistan and is briefly described and illustrated.
...

Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Wasiullah1 and Wiqar Ahmad

...tter content (SOM) under saline soil and yield of rice crop at National Agricultural Research Center (NARC) Islamabad during summer 2016. Pots were induced with salinity of 7.0 dS m-1 and arranged in Completely Randomized Design (CRD) with three replications. Six treatments consisted of a control (no strains) and 5 different strains of cyanobacteria i.e Oscillatory-MMF-1 (Oscillatoria princeps), Leptolyngbaya-MMF-2 (Lyngbya ...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali

...pression of GUS in the basal scutellar regions of immature embryos compared to apical and embryo axis region. Heat shock of 46 oC for 3 minutes increased the transformation efficiency of immature embryos by 26.96%. Growth of infected immature embryos on resting medium after co-cultivation period enhanced the survival of kanamycin resistant calli on selection medium by 93.97%. Agropine strain (EHA101) of Agrobacterium was found 25.67% more effective than octopi...

Ghani Akbar1, Steven Raine2, Allen David McHugh3, Greg Hamilton4 and Qurban Hussain5 

...erbates waterlogging and salinity. Addressing this issue, farmer managed irrigations on commonly used Narrow Bed (NB) with furrow spacings 66 cm and newly introduced Wide Bed (WB) with furrow spacing 130 cm on sandy clay loam, were investigated using the irrigation modelling software, WinSRFR, to explore irrigation management strategies for improving irrigation eficiency. The study revealed existing average application eff...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1,  A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...lenic acid (ALA) and docosahexaenoic acid (DHA) on frozen-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored int...
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
...al except Group A. Blood samples from adult cattle were collected at day 0, 15 and 30 after parturition and in cattle calves at age of 0, 14, 28 and 42. Colostrum sample was collected at 0, 12 and 24 h after parturition. Antibody titer was determined by Complement fixation test. Results showed that highest antibody titer was developed in cattle, colostrum and their calves in group G in which treatment was started 75 days pre...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...of soil and water. Fecal samples from cats at Pet center of UVAS (Lahore, Pakistan) were screened for coccidian parasites through microscopy examination. DNA was extracted from positive fecal samples for coccidian parasites and a T. gondii PCR was performed. Five sets of primers were designed using PrimerSelect tool for PCR to amplify SAG2 gene 5&...
Rehmat Ullah1*, Kalim Ullah2, Inam Ullah3, Muhammad Zafarullah Khan1 and Asif Nawaz1 
...es (70) and farmers were satisfied from the solution of problems they reported (92). Correlation coefficients demonstrate that education, frequency of FAs visits, accessibility of FAs office and establishment of demonstration have highly significant association with provision of new knowledge at (P≤0.01) level of probability. Analysis of variance confirms that most of the farmers with more than 20 hectare land visit the offices of FAs more frequently. Rank ...

Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3* 

...in in vitro functional assays. We compared the anti-tumorigenic properties of these three molecules for tumor volume reduction in xenotransplanted SCID mice and by histopathological and immunohistochemical analyses. Our results demonstrated that fusion protein VEGFR1(D1-D3)-Fc efficiently inhibited
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptame...
Mahmood-Ul-Hassan*, Naeem Fiaz, Muhammad Akhlaq Mudassir and Muhammad Yasin
...arch Institute (SRI), Faisalabad to explore the ratooning ability of five sugarcane varieties/clones under varying harvesting dates of plant crop using randomized complete block design (RCBD) with split plot arrangements. The cane genotypes viz. S2003-US-704, S2006-SP-30, S2003-US-410, S2006-US-321 and HSF 240 (check) were kept in main plots whereas harvesting dates viz. 1st November, 1st December, 1st January, 1<...

Akerele Dare*, Ashaolu Olumuyiwa Fowowe, Sanusi Rahman Akintayo and Egbetade Adedolapo 

... fish production in that same year, it may take up to 2 years to experience a significant output expansion in livestock. Although results suggest that expansion of Agricultural Credit Guarantee Scheme Fund holds substantial positive signals for agricultural growth, especially in the crop and livestock subsector, the somewhat weak effect of the fund on fish production raises some curiosities for policy and strategy. Given that results are ceteris paribus,other ...
Nosheen Rafiq1, Asmatullah Kakar2,*, Arshad Ghani3, Asim Iqbal2, Wali Mohammad Achakzai2, Shagufta Sadozai2, Mohammad Shafiq4 and Mohammad Alam Mengal5 
Shengyan Su, Zaijie Dong*, Wenbin Zhu, Lanmei Wang and Jianjun Fu 
... cross-breeding. A compensatory index matrix for 3×3 full diallel cross combinations used for selection was built to investigate compensatory like growth of each combination. Yes/No(Y/N) effect was determined for specific combination dyads, which there were significant differences in body weight at tagging, but no difference in body weight at harvesting. Among the 36 dyads, 7 had an YN response (19.4%). When the YN eff...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...genic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu...

Shahab e Saqib1*, Himayatullah Khan2, Sanaullah Panezai3, Ubaid Ali4 and Mazhar Ali5 

...sub-district. Multistage sampling technique was used to select the study area. A standardized questionnaire was used to collect data from farming households’ heads. Credit fungibility ratio and ANOVA were used to explore the credit fungibility and credit margin of investment among the groups of farmers. Results showed that a considerable proportion of loan was used in non-agricultural activities by all farmers in general and small farmers in particular. ...
Liana Mihaela Fericean1* and Mihaela Corneanu2
...plants by extracting the sap, and indirectly it is a vector to 16 plant viruses. The study presents data referring to the external morphological characteristics, to the biometrical measurements and to the life cycle of Aphis nasturtii. The researches have been carried out for a period of four years on the potato and for a period of two years on the orchards from Romania. At the Aphis nasturtii species the smallest length of the body found for aph...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
...l of 52 tumor and normal samples were examined, among these, 40 canine and 12 feline samples were analyzed using Sanger sequencing method and RT-qPCR for the mutation and expression profiling of c-Myc gene. The coding region of c-Myc gene (exon2 and 3) did not show any variation in both species. While expression of c-Myc gene was found to be up regulated in different canine (62.5% in mammary adenocarcino...
Jiandong Wu, Miao Ye and Zaigui Wang*
...follows: for adsorption, sample concentration was 2.542 mg·mL-1, pH was 3.7, processing volume was 4 BV; flow rate was 2 BV·h-1; temperature was 25°C. For desorption, elution solvent ethanol-water (95%, v/v) solution was 4.5 BV; flow rate was 2.0 BV·h-1. Flavonoids with content of 68.3% were obtained with a flavonoid recovery of 93.7%. The results showed that AB-8 resin revealed a good ability to purify...
Prof. Dr. Saira Afzal
...th have advantages and disadvantages. Open peer review is performed for scientific quality after publication. It is also known as transparent peer review and public peer review. 
...
Dr. Moazzam Baig Mirza
...s a challenging and life saving facility. 
...
Ayesha Aihetasham1,*, Muhammad Saeed Akhtar1, Maryam Umer1, Khalid Zamir Rasib2 and Muhammad Imran Din3
...hyl ester, 8, 11, 14-Eicosatrienoic acid and 1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester. Extracts of both the plants were found repellent to this termite. LT50 values of O. basilicum and F. vulgare against Heterotermes indicola were 60.91 and 115.9 h, respectively.
...
Müge Aliye Hekimoğlu*, Cüneyt Süzer,Şahin Saka andKürşat Firat
...ort-term application the safe dose for clownfish was 0.4 ml/l phenoxyethanol and 1 ml/l clove oil. For swordtail fish, the ideal amount of phenoxyethanol was 5 ml/l and 6 ml/l clove oil. These safe doses reduce handling stress and mortality.
...
Mahreen Yahya, Noor Abid Saeed*, Sajid Nadeem, Muhammad Hamed and Sajid Shokat 
... Galaxy-13, Millat-11, Lasani-08, Faisalabad-08, and two wheat lines NW-1-8183-8 and NW-3-3341-7 were sown at Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad during the crop season 2015-2016. The aphid infestation started in mid-January on all wheat varieties at stem elongation stage and increased gradually with the growth of plants. Three aphid species were recorded i....
Mubashir Hussain1,Safdar Ali1, Muhammad Naveed Tahir1, Ghulam Abbas Shah1, Ijaz Ahmad2*, Muhammad Aqeel Sarwar3 and Sohail Latif4
...itions of Pothowar. Soil samples for the weed seed bank analysis were taken from the experimental field before wheat sowing from 0-10 cm, 11-20 cm and 21-30 cm soil depth. Two soil weed seed bank determination techniques were compared viz., sieving method and seedling emergence method. The data were collected on the seed density m-2, seed frequency, diversity of weed species and relative importance value. The input and output data was also collected...
Muhammad Qaisar Nawaz
... forage production under salt affected conditions. Two sowing methods i.e. broad cast and drill sowing with 30 cm apart rows and four nitrogen levels (75,100,125 and 150 % of N recommended dose i.e. 150kg ha-) were tested. Recommended dose of PK fertilizer (85-60 PK kg ha-1) was used uniformly with experimental N rates. Data on plant height (132.00 cm), number of plants (91.33 m-2), number of tillers (146.00 m-2), nu...
Muhammad Imran Mahmood* and Muhammad Zubair
...da under drought. In the same way the fresh shoot weight (51.23 g) was found in Sufeda where flooding was applied. While, in Neemthe highest fresh shoot weight (42.75 g) was recorded under drought environment.
...

 Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1

...ent representative water samples and 60 fish samples (20 fish from each site) were collected from three sites i.e. fish-farm, hatchery and Indus River of Punjab, Pakistan. The results showed differences in physico-chemical parameters of water samples collected from all the sites. The concentrations of Zinc (Zn), copper (Cu) and nickel (Ni) were higher in river water as compared to f...
Liushuai Hua1,3, Shiping Gong1,*, Xiangjing Zhong2, Jun Tao2, Yu Chen2 and Jieming Deng2 
... against extinction, but safe overwintering is still technically difficult to captive big-headed turtles. Determining optimal hibernation conditions is important in resolving this problem. In this study, the effects of two important environmental conditions (water depth and availability of sand beds) on changes in body weight and health status in captive big-headed turtles before and after hibernation were evaluated. Results...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
... several hours in tissue samples. For this study piglets without colostrum feeding were selected. Primary cell culture and subculture was performed from the intestinal epithelial cells of piglets. Then the effects of intestinal trefoil factor (TFF3) in intestinal disease was explored. Porcine intestinal epithelial cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mR...
Tahira Perveen1,*, Shaista Emad1, Saara Ahmad2, Zehra Batool1, Sarwat Yousuf1, Sheeza Sheikh1, Sara Qadeer1 and Saida Haider1
...
Synthetic antidepressants are effective drugs for the treatment of psychiatric disorders. Along with the effectiveness these drugs are also associated with some side effects. Since ancient times herbs are used as medicinal purposes. One such herb is Fennel (Foeniculum vulgare). Fennel has many efficacious uses. It is a common herb and spice. It is used in many culinary purposes and also it has therapeutic purposes. In the present study antidepres
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 
...rose, employing a linear salt gradient. SDS-PAGE was used to monitor the purification steps, and protein band was located by immunoblotting technique. Bubaline placental lactogen was purified to homogeneity and showed a molecular weight of about 30 kD. Purified placental lactogen was administered to male albino mice for up to eight weeks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to contr...
Zahid Latif1,*, Kathrin Blasius2-4, Tufail Hussain Tahir7, Muhammad Nasim Khan1, Ghazanfar Ali5, Ansar Ahmed Abbasi6, Abdul Rauf1, Hao Hu8 and Angela M. Kaindl2,3,4
...v>...
Mumtaz Akhtar1, Shabana Naz2,*, Muhamamad Iqbal3, Waqar Azeem4 and Mirza Azhar Beg5
...ve organs. In the winter samples, most of the animals belonged to the middle body weight classes, while in the spring sample majority of specimens belonged to the heaviest weight classes. The comparison of age to body weight resulted in considerable overlap. Age Class I animals never exceeded 70g weight limit. Majority of Age Class II animals weighed less than 86g. Most of the Age Class III animals weighed more than 100g. As...
Muhammad Khaled Siddiq1, Ayesha Riaz2, Muhammad Akbar Khan1, Muhammad Adeeb Babar1, Khalid Mahmood1,* and Muhammad Akhtar1

Muhammad Anas1, Abdul Jabbar1, Muhammad Aqeel Sarwar2*, Raza Ullah1, Muhammad Khubaib Abuzar3, Ijaz Ahmad4 and Sohail Latif5 

...rsity of Agriculture, Faisalabad during the spring season, 2014 in RCB design with triplicate run. The treatments included viz. Four sunflower plants m-2 + 30 mungbean m-2, 6 sunflower plants m-2 + 30 mungbean plants m-2, 8 sunflower plants m-2 + 30 mungbean plants m-2, 4 plants m-2 of sunflower alone, 6 plants m-2 of sunflower alone, 8 plants m-2 of sunflower alone and 30 plants m-2 of mungbean alone. Plant population was maintained by keeping 75 cm apart sin...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
...ed laser desorption/ ionisation time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS) and bioinformatics technique were used for characterize the differential expressed proteins. Almost 78 patho-stress responsive proteins which expression level more than 1.5-fold were identified, where 50 proteins were up-regulated and 28 proteins were down-regulated; The identified proteins were categorized into 16 classes, which are mainly including energy metabolism, g...

Shaista Naz* and Noor Paio Khan 

...ome was derived from the sale of milk, milk products (such as yogurt, butter and butter oil), other products (like farm yard manure and dung cakes) and animals which included calves, goats and sheep mainly. The major share of livestock income was accrued from the animal’s sale followed by milk and milk products, respectively. The domestically consumed livestock products comprised of milk, milk products and other produc...

Muhammad Aqeel Sarwar1,2*, Muhammad Tahir2, Abid Ali3, Manzoor Hussain3, Muhammad Waheed Anwar4, Muhammad Khubaib Abuzar5 and Ijaz Ahmad

...rsity of Agriculture, Faisalabad. There were two factors, green manuring (No green manuring, moringa green manuring and Jantar green manuring) and different levels of NPK fertilizers (no fertilizers, 50% of RDF, 100% of RDF). The experiment was laid out in Randomized Complete Block Design with split plot arrangement in triplicate run. Results from the experiment revealed that both the factors significantly affected all the parameters under study while their in...

Haidar Ali* and Malik Muhammad Shafi 

...rs in Peshawar Valley. A sample of 201 small farmers were selected using random sampling technique and data were collected through a pre-tested interview schedule. This study focuses on two selected districts i.e. Peshawar, and Charsadda. Four villages were selected two each from the sampled districts. Analysis showed that the coefficients of household s...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*

 

...e isolated from 83 fecal samples. The organisms were inoculated on MacConkey agar and EMB agar and identified via biochemical tests. Polymerase chain reaction was used to detect representative virulence factors or genes, including E.coli adherence factor (K88, CS31A, afaE-8), toxins (estA, estB, Stxl, Stx2, EAST1), pathogenicity island (eaeA, irp2, ETT2) and outer membrane protein (ompA). Moreover O-antigen serotype wa...

Imran Ali Rajput1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1, Imran Khatri1 and Abdul Mubeen Lodhi2 

Imtiaz Ahmed, Muhammad Abbas Khan, Noorullah Khan, Naveed Ahmed, Abdul Waheed, Fazal Yazdan Saleem, Sajjad Khan and Sohail Aslam 

Jie Wu1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yingying Liu1, Yanzhen Bu1,* and Hongxing Niu1,*
...ndangered bird. Aimed to save and restore the small population in Henan Province, China, a reintroduction program was conducted in the Dongzhai National Nature Reserve in 2007. Wild birds bred in semi-wilderness were reintroduced. Habitat selection and breeding ecology of crested ibis in Dongzhai National Nature Reserve has not been studied. To clarify the breeding status of the crested ibis and to provide more reference for the reintroduction of this species,...
Fahad Tanveer1, Adnan Afzal2, Muhammad Adeel3*, Sana Shahid4 and Maham Masood5
...-probability convenience sampling technique who fulfilled the specific inclusion and exclusion criteria were randomly allocated to three groups; group 1 received SNAGs, group 2 Maitland mobilization and group 3 conventional treatment. Baseline, post-intervention and follow up readings were taken through numeric pain rating scale (NPRS) for pain intensity, neck disability index (NDI) for functional status,goniometer for range...

Riaz Alam* and Muhammad Sajid 

...r-layerage. The daughter saplings of cultivar Manzanilla took less number of days (47.94) to root appearance, produced more rooting percentage (38.89%), number of roots (4.31), root length (4.61 cm) and root weight (1.77g) with more number of re-sprouts (3.18) and shoot length (5.28 cm). The time intervals of air layering also significantly influenced the rooting and growth attributes of olive cultivars. Mid August, proved to be the most favorable time for air...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...semen collection was the same in both methods. Also, there were no significant differences in semen parameters such as sperm volume, motility, morphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to ...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...the semen quality. Semen samples were individually diluted with egg yolk extender and tested for individual motility, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed semen. Data were subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), progressive motility (20.25 ± 0.47%), plasma membrane integrity (82.25 ± 0.43%), a...
Shumaila Ashfaq1, Liaqat Ali2*, Muhammad Ashraf Zia2, Rizwan Ahmad Khan2 and Mehtash Butt3
...ant female undergoing caesarean section due to its safety than general anaesthesia. Post dural puncture headache (PDPH) is commonly occurring problem associated with spinal anaesthesia and causes a considerable morbidity. PDPH depends on several factor and various methods have been used to reduce and treat the pain of PDPH. We evaluated effects of hydrocortisone on the treatment of PDPH in obstetrical patient.To compare mean...

H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2 

...mens and 28 (60.9%) milk samples. Brucella cultures were further identified on molecular basis using universal and Bruce ladder PCR. 

...
Anan Kenthaoand Pornpimol Jearranaiprepame*
..., and discriminated fish samples into three major groups following to their collecting drainages. Furthermore, 96.29 and 90.56% of fish samples were correctly classified into their respect populations with original and cross-validated tests, respectively. The reliable morphometric variations in the present study suggest that management unit of C. apogon should define relied on the isolation of river drainage. Moreover...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
...n. A total of 1041 stool samples were examined from January 2006 to December 2008 using direct smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with single parasite and seventy four (64.3%) with multiple infections. Entamoeba histolytica 30.5% (n=77/252), Giardia lamblia 15.0% (n=38/252), Ascari...
Ümit Kumbıçak, Zübeyde Kumbıçak* and Gülnare Huseynli
... determined based on Giemsa-stained testicular chromosomes for the first time. Results of spermatogonial metaphases showed that the chromosome number and the karyotype formula of the species was 2n♂=35 (X1X2X30) which is the first record for the family Hersiliidae. Both autosomes and sex chromosomes were acrocentric. The lengths of the chromosomes indicated no significant difference between autosomal pairs, however, X
Muhsan Ali Kalhoro1,2, Danling Tang1,*, Ye Hai Jun1, Morozov Evgeny1, Sufen Wang1 and Muhammad Aslam Buzdar
Hafiz Muhammad Ishaq1, Muhammad Shahzad2, Xiaokang Wu3, Chaofeng Ma4 and Jiru Xu1,*
...n and narrows the air passages. Present study aimed to characterize the gut microbiota of asthma patients and to investigate the alteration in similarity and diversity of gut microbial composition with comparison of healthy controls. Fecal samples from fifteen patients and five healthy individuals were collected. PCR-DGGE, by using universal primers focusing V3 region of 16S rRNA gene, was...
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
... results it would be advisable to conduct studies in a larger population of cows with consideration of other breeds.

 

...

Rafiullah*, Muhammad Jamal Khan and Dost Muhammad 

...were first germinated in sand culture and then three weeks old seedlings (7.5 cm) were transferred to water tubs containing Hoagland solution without P. The treatments included 0, 18, 36, 72, 144 and 216 mM as KH2PO4 solution foliarly applied three times with one week intervals starting after two weeks of seedlings transplantation. Each treatment unit consisted of 10 seedlings arranged in CR design with three replications. Plant height, shoot and root dry biom...
Muhammad Siraj-ud-Din1,2, Riaz Aziz Minhas1, Usman Ali3,*, Mayoor Khan2, Muhammad Siddique Awan1, Nuzhat Shafi1 and Basharat Ahmad1
... collected by using scan sampling technique and also collected from local people, hunters and shepherds (n=78). During scan sampling, focused feeding animals were observed with the help of a telescope and spotting scope. Ladakh urial preferred montane dry sub-tropical scrub zone habitat with 41.87% evidences of its presence followed by alpine meadows/alpine scrub zone (21.14%), sub-alpine scrub zone (13.41%), and dry tempera...
Sayyed Ghyour Abbas1, Muhammad Adeeb Babar1,*, Muhammad Akbar Khan1, Kiran Aftab2, Ayesha Riaz3, Abdul Ghaffar4 and Muhammad Akhtar1
...unjabicus, T. cf. salmontanus, Elachistoceras khauristanensis and Gazella lydekkeri. The boselaphines are predominant at the type locality. The taxa are consistent with a Late Miocene-Early Pliocene age of the deposits. The findings expand our knowledge on the dental anatomic features of the described species that existed in the Middle Siwalik Subgroup during the Late Miocene-Early Pliocene.
...
Serap Gelibolu1,*, Yasemen Yanar2, M. Ayce Genc3 and Ercument Genc4
...he impact of mannan-oligosaccharide (MOS) on growth performance, body physiology and tissue morphology of gilthead seabream (Sparus aurata). Treatment of fish with MOS-feed shown a significant increase in live weight and protein efficiency rates when were directly compared with mock-treated fish control. However, there was no statistically supported level of significance was observed for growth rates and feed conversion rates among groups. Improved live...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...ly, Huwa-San TR50 can be safely used in biological control programs, as it has little or no effects on biological control agents such as predatory mite, N. cucumeris, which is widely associated with the infestation of two spotted spider mite, T. urticae on tomato plants.
...
Rana Hanan1*, Muhammad Waqar Shafi1, Sumair Anwar2 and Numrah Nawaz1
... silent cause of high causalities around the globe. Both hyper and pre-hypertension affect all ages and professions, and are as common as flu infections by affecting 1 in every 5 persons. In order to determine frequency of hypertension and pre-hypertension among medical students and their association with various variable and fixed risk factors, this study was designed. The cross sectional study of 4 months was carried out on the students of Shalamar Medical a...
Xiaoting Zhang1,2, Jiaxiang Wang1,2,*, Peng Li1,2,* and Lixun Ye1,2
...ue formed a layer of serosa on the surface of the lung. The parenchyma of the lung consisted of bronchial levels, atria and respiratory capillaries. The bronchi were divided into the primary, secondary and tertiary bronchi. The wall of the tertiary bronchus had openings for the atria and respiratory capillaries. The respiratory capillaries were surrounded by a rich blood capillary bed. Ghrelin-ip cells were located among the tertiary bronchus muco
Wen-Feng Li, Xiao-Yan Wang, Ying-Kun Huang*, Rong-Yue Zhang, Jiong Yin, Zhi-Ming Luoand Hong-Li Shan
...i>(Snellen) and Chilo sacchariphagus (Bojer) were measured in pesticide–treated and untreated areas during 2014-2016. The results indicated that the in untreated areas, percent dead-heart was 8.30% - 58.83%, the percent stalk injured was 26.73% - 96.67%, the percent internodes bored was 4.40% - 25.87%, and the effective stem number was reduced by 4440-21900 stalk/ha. Measured yield and sugar yield loss results showed cane yield was reduced ...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...ucted. Eventually, MTT assay was performed to observe the cells proliferation. The results showed that miR-122 and miR-449a were highly-expressed in testis tissues (p<0.01). The expression levels of miR-122 and miR-449a in the 24-month-old testes were higher than those in the neonatal and 12-month-old groups during the testicular maturation process, however, PDK4, DUSP4 and FKBP1B expression levels were decreased (p<0.01). Furthermor...
Rana Fartab Shoukat1, Shoaib Freed1,*, Kanwar Waqas Ahmad1 and Ateeq-ur-Rehman2
... Ma-11.1, Ma-2.4)and Isaria fumosorosea (isolates If-2.3, If-02) and chemical insecticides i.e., bifenthrin, lambda cyhalothrin, imidacloprid, triazophos, spinosad, pyriproxyfen and nitrinpyrum, as larvicides against C. pipiens. Highest larval percent mortality (73.3 ± 4.7) was observed after application of Ma-11.1(LC40) + nitenpyram (LC40) mixtures under laboratory and field...

Safdar Ali1*, Ghulam Abbas Shah1, Muhammad Naveed Tahir1, Shahid Mehmood2, Asim Gulzar3 , Ijaz Ahmad4 and Bashir Ahmad Khan5 

...stemscombined with glyphosate herbicide on soil moisture content,bulk density and porosity associated with wheat grain yield were evaluated. Seven tillage treatments were applied in the fallow periods (summer seasons) of both years before sowing of wheat crop. These tillage treatments were (i) Once Mould board plowing followed by 8 cultivations with planker (1 MBP + 8 CS) as a check, (ii) No-till followed by twice application of glypho...
Tariq Mahmood*1, Syed Wasif Ahmed Shah1, Muhammad Rais1, Hira Fatima1, Faraz Akrim1 and Muhammad Sajid Nadeem2
...ected from five selected sampling sites by searching and identifying nests of different bird species in various vegetation types and observing birds on the nest. The nests were monitored for breeding activity of the particular bird species through clutch size, incubation period, and hatching success. Sixty-two active nests of various bird species were recorded; common myna Acredotheris tristis, house crow Corvus splendens, Indian robin Saxicol...
Faiza Ambreen*1 and Muhammad Javed2
...cus by using Comet assay. The 96-h LC50 value of pesticide mixture was estimated for 180-old fingerlings of O. niloticusin a static system and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fish were exposed to these concentrations, separately in glass aquaria for 70 day...
Ghazala Saeed1, Rabia Wajid1* and Ayesha Yousaf Dar2
...as eclampsia. Eclampsia isassociated with multiple maternal and fetal complications. Eclampsiais an obstetric emergency and quick decision making is required to save lives. The objective of the study was to compare the maternal mortality after cesarean section versus vaginal delivery among eclampsiapatients.It is a descriptive case series conducted in Department of Obstetrics and Gynecolog...

Naila Riaz1 , Hafiz Muhammad Tahir2* , Khizar Samiullah3

...om dose. However, at the same venom dose, 100% mortality was achieved at 8 hr posttreatment when applied topically. Similarly, oral administration of 1µl and 0.5µl venom caused 100% mortality at 8 hr post-treatment compared to 10 hr and 12 hr post-treatment of topical application, respectively. It is concluded that oral treatment of venom was more efficient compared to its topical treatment with same doses of tre...

Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2

 

... unemployed. Parental consanguinity was another important determinant studied and was identified that three of four offsprings of cousin marriage were schizophrenic. It was concluded from the present study that along with inherited nature of schizophrenia importance of shared environment cannot be ruled out. There is an urgent need to educate general population about the consequences of disorder so that people take it cautiously and it may treated early and it...

Haji Muhammad1, 2, Zafar Iqbal1, Qamar Bashir2, Muhammad Amir Hanif3

...es of Indus River in Taunsa barrage from Punjab, Pakistan. The value of length weight relationship (LWR) equation and condition factor (K) ranged W= -0.249 TL0.79 – W= -2.803 TL3.55 and 0.07 – 1.69 respectively. The objective of this study was to determine LWR and condition factor of cat fish species of Indus River that could help in
conservation and management of ichthyofauna in the Indus Rivers, Pakistan

...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
...ay, blood and tibia bone samples from 5 birds per treatment were collected. Total weight gain and dry matter intake in chicks fed rations I, II and III were 1861, 1736 and 2023 gm/bird and 3242, 3284 and 3265 gm/bird, respectively. The chicks fed R-III got 16.00 and 8.00% higher weight gain compared to chicks fed R-II and R-I, respectively. Feed: gain ratio (gm/gm) was found best (P<0.05) in chicks fed R-III (1.61) and poorer in chicks on R-II (1.89). The c...

Muhammad Khalid, Muhammad Naeem*

...BG), body depth (BD), Dorsal fin length (DFL), pectoral fin length (PtFL), pelvic fin length (PvFL), anal fin length (AFL), eye diameter (ED), caudal fin length (CFL), caudal fin width (CFW) of farmed collected Ctenopharyngodonidella were studied to know their correlation with reference to size (TL and W), and types of growth pattern. Correlation coefficient (r) value (0.984) shows high significant relationship between total length and wet body weight, while g...

Manzoor Hussaina,b

...nce material in sexual assault cases to get the DNA profile of the perpetrator. With the increase in post-coital interval, the possibility to get the autosomal DNA STR profile of the perpetrator diminishes rapidly from an alive victim. This becomes more serious problem especially when victims of rape cases provide vaginal swabs after several days of the incidence. The study was designed to find the time interval at which autosomal STR system fails to generate ...

Naeem Iftikhar1, Qamar Zaman Qamar2, Usman Ali3, Muhammad Siddique Awan4, Syeda Shaista Bibi4, Riaz Aziz Minhas4*

... population of Cheer pheasant in Pakistan. Study was conducted from July 2010 to June 2011 to determine the potential of these sites as a possible focal point to start Cheer pheasant conservation activities in AJK. A total of 579 individuals was estimated from study sites. Highest population (n=434) was recorded at Qazi Nag Game Reserve, followed by Phalla Game Reserve (n=117) while minimum population (n=28) was noted at Pir...

 Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*

...f bacteria isolated from saline environment and to check their biofilm forming ability under nickel stress. Our previous results showed that these bacteria are capable of producing proline, glycine betaine, choline and other osmolytes under NaCl stress. On the basis of those results it is hypothesized that this osmolyte production ability may help them in nickel resistance under Ni salt stress. This study was centered on ass...

 Bushra Mazhar1, Nusrat Jahan1, Nazish Mazhar Ali1, Saiqa andleeb2, Shaukat Ali2*

...sidered natural but also safe for human health.

...
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
 
... male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amongst serum biochemical parameters triglycerides (P = 0.02) in CGP55845 treated mice showed signif...
Qingying Jia1, Hui Wang1, Tianhong Wang1, Na Dong1, Shuguang Ren1,2, Xiaolong Yang1, Jingze Liu1,* and Zhijun Yu1,3,*
...
The tick Haemaphysalis longicornis is of great medical and veterinary importance, and can transmit a great diversity of pathogens. The current study investigated the effects of temperature on reproductive fitness of the engorged H. longicornis, results indicated that the engorged H. longicornis could survive all treatments at 0°C and 4°C, and pre-ovipositon periods were prolonged after 0°C, 4°C and 40°C treatment, ...
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
...ile in control fish, the same for both the organs were observed as 0.085±0.002 and 0.112±0.002 UmL-1, respectively.
...
Ikramul Haq1, Aneela Zameer Durrani*1, Muhammad Sarwar Khan1, Muhammad Hassan Mushtaq2, Imtiaz Ahmad3, Amjad Khan2 and Mehboob Ali1
...447 diarrheic foals were sampled for isolation of bacteria. During the study 294 faecal samples (horse n=217, mules n=23 and donkey n=54) were diagnosed positive for bacteria. The overall prevalence of diarrhea was 72.56% with bacteria in 65.77% of diarrheic cases. The prevalence of different bacterial species shows that E. coli was the most prevalent bacteria with 48.77% as compared to Clostridium perfringens ...
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
... black cumin (Nigella sativa oil; NSO) and insulin. Alloxan induced diabetic rats were post treated with plant extracts (150 mg/kg) and serum samples were collected from overnight fasted diabetic rats on day 15 to observe thepercent changes in blood glucose levels and lipid profile. Garlic extracts have shown the significant (p<0.001) hypoglycemic activity with no considerable effect on mean body weight of rats (ns...
Abu ul Hassan Faiz1,*, Fakhar-i-Abbas2 and Lariab Zahra Faiz2
...s in eight type of soil (sand, loamy sand, sandy loam, sandy clay loam, loam, clay loam, silt loam, silty clay loam). A total of 1571 animals were captured belonging to species of Nesokia indica, Mus musculus, Mus budooga, Millardia meltada, Tatera indica and Golunda elliotti. The maximum number of rodents was captured in loamy soil type wi...
Zahid Farooq1, Sufi Muhammad Akram2, Muhammad Saleem Khan3,* and Muhammad Wajid4
...tus), Sind long-nose sand snake (Lytorhynchus paradoxus)and Afghan awl-head snake(L. ridgewayi)(0.38%; N = 2) were the least abundant. Snakes were more frequent in croplands and least frequent in barren areas.
...

Niamat Ullah Khan1*, Abdul Aziz Khakwani2, Sami Ullah2, Najeeb Ullah2, Abdur Rauf3 and Inam Ullah2

...better plant population, save irrigation water, cut cultivation costs and increases cotton yield and quality on sustainable basis. Field trial was conducted in 2013 and 2014 at Cotton Research Station, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan to study the effect of tillage systems [ridge tillage (RT) and flat tillage (FT)] and 5 nitrogen levels (0, 50, 100, 150, 200 kgN.ha-1) on cotton lint yields, quality and earliness. Experiment was laid out in a RCB ...

Soraya Khosravian Dehordi*, Abdolnaser Mohebbi and Kahin Shahanipour

...I (control) given (1 ml) saline. All doses was orally (gavage) given to rates.Protein carbonyls (PC) andthiobarbituric acid reactive substances (TBARS),superoxide dismutase (SOD) glutathione peroxidase (GPx) and catalase (CAT) values in spleen were measured. An increase in TBARS and PC levels was obtained in rats' spleen of the Cd-group than controls and nano-Se decreased this effect close to the control. The CAT values of all groups did not differ significant...

 Addishiwot Fekdu, Afework Bekele and Demeke Datiko*

...i (1), and Crocidura fumosa (4). Tachyoryctes macrocephalus was recorded as frequently observed species. Four endemic species of rodents were identified in the area (Lophuromys melanonyx, Arvicanthis blicki, Dendromus lovati and Tachyoryctes macrocephalus). The distribution and abundance of species varied between exclosure and control sites, and between seasons. Livestock grazing in the area was the major problem encountered affecting the rodent population. As...

Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz

...ia and to optimize the assay conditions under which enzyme possessed maximum activity. Two bacterial isolates XPB-GS02 and XPB-CW01 with maximum activity ratio showed 18.3 and 12.2 U/ml xylanase activity, respectively. Maximum xylanase was produced in medium M1 containing xylan after 48 hours of incubation. Maximum amount of enzyme was produced by XPB-GS02 at 45ᵒC, pH 9 using 1% xylan concentration, while XPB-CW01 produced maximum amount of xylanase at 5...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4 and Lorenzo Gallus
...iv>The effect of varying salinity levels (15‰ to 40‰ with 5‰ increment) on growth, feed efficiency and meat quality of red tilapia, Oreochromis sp. (mean body weight 5±0.07g) were investigated. Juveniles of red tilapia were randomly distributed into seawater tanks (60 cm × 30 cm × 45 cm each). Ten fish were stocked in each tank with 2 replications. Fish were fed with commercial floating pellet (35% protein) at 3% ...
Yakut Gevrekçi* and Çiğdem Takma
...on tree analysis is necessary to provide preliminary information in breeding programs to be performed. 
...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
Muhammad Usman Saleem1, Saima Masood1,*, Hafsa Zaneb1, Aneela Zameer Durrani2, Asim Aslam3, Kamran Ashraf4, Habib-ur-Rehman5, Muti-ur-Rehman3 and Muhammad Shabir Shaheen6
...ng effects of mannanoligosaccharide (MOS) and organic acid blend (OAB) supplementation individually and in combination on carcass yield, small intestinal microarchitecture and serum biochemistry in Hubbard chicks. A total of 128 one day-old chicks were divided equally in four different groups with each group having 4 replicates. The number of birds per group were 32 whereas the number of birds per replicate were 8.The first group was kept as control (CONT) whe...
Abd El-Nasser Ahmed Mohammed*
...nsplanted ovaries. Blood samples were taken for analysis of complete blood picture. The results indicated presence of a neovascular capillary network around transplanted ovarian tissues. In addition, visible antral follicles and corpora lutea was demonstrated on the ovarian tissues upon transplantation. Histological examination indicated that transplanted young ovarian tissues restored function better than elder ones. It could be concluded that unilateral ovar...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4

 

...(trachea, lungs, and air sacs) were taken for histological analyses. All the infected subjects showed clinical signs of varying severity. Co-infected groups showed the most obvious clinical signs, associated with significant higher mortality and respiratory organ abnormalities, in comparison with the mono-infected groups (P<0.05). There was a non-significant (P>0.05) effect of the inoculation time intervals between vNDV and E. coli inoculation (no...
Tania Ahmed Shakoori1,*, Muhammad Saqib Saeed2, Asif Hanif2, Saima Mukhtar3 and Ayesha Ghauri4 
...udy after obtaining necessary permissions from the ethical board of the hospital. COPD was diagnosed on the basis of clinical symptoms and a post bronchodilator values of FEV1/FVC<0.70 on spirometry. Frequent exacerbator’s were defined as patients experiencing 2 or more exacerbation episodes per year, which merited visited to the hospital. Average number of hospital visits for exacerbations per year, smoking history, family history of smoking related ...

Muhammad Tariq1*, Muhammad Khawar Hussain1, Zilakat Khan Malik2 and Noor Jehan1 

...villages of district Charsadda. Out of 382 producers 176 were located in Tebana Dhere and 206 in Sarkai village. It is find out that 47% of land area was under the cultivation of strawberry with average production of 2898 kilogram per acre. The per acre average values of land preparation, NPK fertilizer, labor, seedling, farm yard manure and pesticides were recorded 6.00 hrs, 248 kgs, 143 man days, 24413 seedling, 1.48 trollies and 1.21 litres respectively. Th...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...e and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple and accurate test for the rapid diagnosis of FMD. This study has been done initially with FMD known serotypes (O, A, Asia1) already in use at animal health.Viral RNAs were extracted using R Neasy Minikit (Qiagen) according to instruction manual. After extraction RNAs were eluted in 60 ul elution bu...
Lowell Gustafson
...he would be the first to say that is not the final word about ultimate reality.
...

Waqas Liaqat1, Mohammad Akmal1* and Jawad Ali 

...ear, grains per ear, thousand grain weight) were also adversely affected by sowing dates, which decreases both biomass and grain yield. Varieties did differ in phenology (i.e. emergence, silking, tasseling, maturity) and morphology (i.e. height, leaf area, ear height, cobs per plant and its weight) that showed differences in biomass and grain yields. The study suggested that a delay in sowing of maize crop from June in the region after Wheat or Berseem harvest...
Ann Milliken Pederson
...nd this book attempts to satisfy the thirst or at least provide the water to drink. In a world where fragmentation, brutality, and chaos mark the lives of so many, it is no wonder that people long for wholeness, healing, transformation, and an encounter with the ultimate. Ted Peters’ new book locates the vocation of humankind within their cosmic home, brings together the disciplines (humanities, social sciences, natural sciences) into a holistic integrat...

Muhammad Zamin* and Abdul Mateen Khattak 

...nservation and to combat salinity problem. For this purpose, various ecotypes of Sporobolus spicatus were investigated by conducting mowing tests in the year 2013 under UAE climatic conditions. Fifty ecotypes of Sporobolus spicatus were cut at various heights, i.e. 1, 2, 3, 4 and 5 cm. Significant differences were found among various ecotypes and mowing heights for different growth and quality parameters including green cover, canopy stiffness, leaf length and...
Ted Peters
...y and human history? No, say big historians; we cannot ask the God question. Religion maybe, but certainly not God. It follows that we need more than just Big History which looks at nature through scientific lenses. We need Cosmic History to pose the God question, and even to discern human meaning within an otherwise impersonal universe. In this article, I entertain criticisms of my book, God in Cosmic History, raised by Lowell Gustafson, President of t...

Ayesha Bibi*, Musharaf Ahmad and Shaukat Hussain 

...al of 100 collected weed samples were analyzed in laboratory for the presence of bacteria. The samples were crushed homogenized in 0.85% TBE buffer and platted on YDC (Yeast extract-Dextrose-CaCO3) for bacterial growth. For identification of the characteristic bacterial colonies Cmm-specific primers CMM-5 and CMM-6 were used for amplification of 614bp product from pat-1 gene of bacterial plasmid. The whole bacterial DNA was ...
...elated with group of the same composition at Safari zoo Lahore. The frequency of different behavior patterns displayed by these lions was studied at both study sites for three hours once a week by instantaneous sampling method for 16 weeks, from 18th April to 8th July in 2014. The frequency of stereotypic behavior including auto mutilation, refusal of food stuff, fece...
Shahid Mehmood1,*, Bushra Nisar Khan1, Hassan Raza1, Rida Ahmad1, Azhar Muhammad3, Zulfiqar Ali2, Farhan Abid1, Fehmeeda Bibi4 and Syed Maviz Ahmed5
...eight (8) (11.3%) and passage migrant one (1) (1.4%). More birds were observed in winter season compared to summer season. The most dominant species were house crow, Corvus splendens (n=2518), house sparrow, Passer domesticus (n=772), common myna, Acridotheres tristis (n=327), black-crowned night heron, Nycticorax nycticorax (n=190) and jungle babbler, Turdoides striatus (n=186). Among 71 species, Alexandrine parakeet, Psi...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4, Lorenzo Gallus4 and Syed Sajjad A. Shah2,3
...ducted to assess optimal salinity level among 0‰, 5‰, 10‰, 15‰, 20‰ and 25‰ for successful breeding of Nile tilapia, Oreochromis niloticus. The duration of study was 56 days. Brooders (48) having mean weight (male 162±0.2 g and female 160±2.5 g) were selected and stocked into hapa nets in 12 fiberglass tanks (2000-liter). Ratio among male and female was 1:3. They were fed with commercial floating p...
Tasleem Akhtar*, Muhammad Asif Aziz, Muhammad Naeem, Muhammad Sheraz Ahmedand Imran Bodlah
...> (1.11%) and Apis dorsata (0.98%). Peak activity of the insect visitors was observed at the mid of the day i.e., 12:00 pm. The activity of managed A. mellifera started to increase from the third week of blooming (20th Jan, 2015) and reached to maximum in the sixth week (10thFeb, 2015). Among the abiotic environmental factors, temperature had a strong, significant and positive correlation with the foraging activity of...

Muhammad Luqman1,3, Raheel Saqib2*, Xu Shiwei3 and Yu Wen3 

...d farm family. The total sample size of the study was 300 (150 male and 150 female spouses). A designed structured questionnaire was prepared for the data collection as the research instrument. The data thus collected were coded on SPSS for analysis and interpretation. Results showed that there is significant difference in age and educational status of male heads and their spouses. Majority of the farm families (39.3%) earn income for their livelihoods both fr...

Imran Khan*, Zahir Shah, Wiqar Ahmad, Farmanullah Khan and Muhammad Sharif 

...d the porosity by 9-14%, saturation water by 9-20% and available water by 19-29%. However the INM5:75,10:50, 15:25, closely followed the FYM5,10,15,20 with reduction in BD by 9-11% and increased in porosity by 11-13%, saturation water by 14-17% and available water by 25-30%. All interactions remained non-significant. These results concluded that integrated nutrient management improved the soil OM, micro-nutrients content and...

Ronaq Zaman*, Shahid Ali and Inam Ullah 

...ndents using multi-stage sampling technique through well-structure interview schedule. For the estimation of Stochastic frontier tans-log production function, maximum likelihood estimation technique was used. To ensure the abnormality of data set Chow test and Dummy variable test was employed for any possible structure break across the broiler farms. Results indicated that there were significant structural breaks across the sa
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Ghulam Dastagir4, Sara Ferrando5, Lorenzo Gallus5, Asad Ali Muhammad1,6, Abdul Jabbar2 and Khalil-ur-Rehman2 
...iv>Assessment of optimum salinity level (10‰, 15‰, 20‰, 25‰ and 30‰) for growth performance, food conversion, body composition and survival rate of Nile tilapia, Oreochromis niloticus (mean body weight 4.5±0.5 g) were investigated. Fingerlings were randomly distributed into the glass tanks (60 x 30 x 45 cm each). Ten fish per tank were stocked with two replications. Fish were fed with commercial floating pellet ...
John Martin Fischer
...hey are motivated by the same basic intuitive ideas. It is also important to see how they are the same in significant ways.
...
Meghan Griffith
...he incompatibility of causal determinism and freedom to do otherwise” (1). Comparisons between foreknowledge and determinism appear throughout the book, typically when Fischer is defending incompatibilist arguments regarding foreknowledge and freedom to do otherwise (i.e., alternatives). But in an intriguing discussion in the introductory chapter, Fischer draws a comparison between determinism and foreknowledge with respect to moral responsibility...
David P. Hunt
...s previously published essays on the problem—five earlier papers, and six that appeared just within the last eight years. It also opens with a substantial overview, itself worth the price of admission, written specially for the book.
...
Katherin Rogers
...dquo;) in Our Fate: Essays on God and Free Will, briefly mentions a solution which is somewhat similar to the one I take to be the most successful way of dealing with the dilemma of human freedom and divine foreknowledge. He presents the solution as Ockhamism plus “eternalism” and dismisses it quickly in that it requires an “eminently contestable metaphysical picture about the nature of time”.1 I will defend Ansel...

Afsheen Masood1*, Rubab Musarrat2, Shama Mazahir3 and Adeela Ashraf4  

...fe and perceived marital satisfaction in parents of children with leukemia with that of normal cohort. Case control study design was used. The sample consisted of N=120 parents out of which n=60 were the parents of children with leukemia and n=60 were the parents of normal children, , taken as normal cohort and drawn through non-probability purposive sampling procedure. Both cohorts were m...

Aneeza Jamshed1* and Syed Amir Gillani2 

...rough multistage cluster sampling. A quasi-experimental study was conducted in the time period of 6 months from April 2014 to Sep 2014. Data analysis was done with SPSS 20. After describing the important variables, average percentages were used to find out the difference between pre and post awareness performances and t score and p value was calculated. All the students (150) were boys, 98% belonged to a lower middle class family with the age of 9-12 years. Du...

Arooj Fatima1* and Ashfaq Ahmed

...hore. The study included sample size of 50 subjects diagnosed with rotator cuff tendinopathy. Patients included in study were allocated randomly into; Group-A: This group was treated with routine physiotherapy treatment. Group-B: In this group, subjects were trained with eccentric loading exercises along with routine physiotherapy treatment. The outcome measures were pain intensity measured by using Visual Analogue Scale (VAS) and functional outcome measured b...

Zehra Mohsin1*, Sarah Shahed2 and Talat Sohail

...s in public hospitals. A sample of 160 nurses of age (M=33.48, SD=9.18) was selected from public hospitals of Lahore. The nurses completed a demographic information form, Multidimensional Scale of Perceived Social Supportand Professional Quality of Life Scale. The data were analyzed using SPSS version 20. Two demographic variables, age and experience had significant positive correlation with compassion fatigue, r =.252, n=160, p <.01 and r =.256, n=160, p &...

Uzma Zahid 

...r relationship with life-satisfaction in final year medical school students of both genders. This cross-sectional study was carried out in final year students of a medical college in Rawalpindi, Pakistan using 24 items revised English Version of Career Aspiration Scale (CAS) and 5 items Satisfaction with Life Scale (SWLS). This study was conducted on total 88 final year medical school students, among them 44 were males and 44 were females. Findings show signif...

Attia Anwar1, Sajida Malik2*, Zobia Usman1 and Sadia Chiragh3 

...ks post-treatment, blood samples were collected by cardiac puncture from both groups and tested for Hb (%), WBC, RBC and platelet counts, SGPT, alkaline phosphatse, serum uric acid, blood urea and serum creatinine. All the numerical data were expressed as mean± SD. The values in two groups were analyzed with help of unpaired t-test. A p-value ˂ 0.05 was considered significant. After 24 weeks of treatment with berberine hydrochloride, no significant...
Richard Swinburne
...owledge of any future causally undetermined contingent event, and so – if there are any such events - could not be essentially omniscient, in the sense of having infallible knowledge of all true propositions. Given the assumption that human free actions are causally underdetermined, this entails that such a God cannot at any time have infallible knowledge of which future actions humans will do freely. 
...
Alan R. Rhoda
...ublished collection of essays on this topic (Fischer 2016). Given my space limitations and time constraints, I have decided to focus my comments on what he dubs the “Bootstrapping View” in section V of the volume’s introductory essay. The view is a novel proposal for how God might be able to have infallible foreknowledge of the occurrences of caus...
John Martin Fischer
...houghtful and generous essay by Meghan Griffith. She raises various important issues, which are worth thinking seriously about, but I want to focus on what I take to be her main point. Griffith agrees with me that it is plausible that God’s foreknowledge is incompatible with human freedom to do otherwise. She also agrees with me that this still leaves the question of whether God’s foreknowledge rules out acting freely (and moral responsibility). I ...

Shahid Iqbal1*, Amir Nawaz Khan2, Mushtaq Ahmad Jadoon3 and Intikhab Alam3 

...villages of District Charsadda. The study findings reveal a significant difference in the area under wheat, maize and vegetables after the flood. At the same time, the area under sugarcane and fodder had also decreased to some extent. Similarly, the use of chemical fertilizers and farm yard manure was also decreased due to decrease in the income of the households after the floods-2010 while an increase, though non-significan...
Muhammad Hafeez-ur-Rehman1,Farzana Abbas2,Ghulam Abbas3,*, Arshad Javid4, Ali Hussain4, Syedah Andleeb5,Khalid Javed Iqbal6 and Muhammad Akmal1

 

Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...is test using convenient sampling technique. Biochemical examination was performed to isolate S. aureus that further were tested Penicillin, Glycopeptide, and Cephalosporin classes of antibiotics for confirmation of MDR isolates using disk diffusion method. MDR were tested in vitro for response against various antibiotics. A questionnaire was filled for estimation of association of assumed risk factors with prevalence. Nonparametric tests at 5% p...
Samra Imran1,* and Afifa Tanweer2
... gender and greater meal satisfaction was found to be associated with better biochemical indices. Most participants reported partial compliance to GFD. Compliant participants reported an increased consumption of junk food whereas, non-compliant patients, consumed significantly higher intakes of meat and fat. Regular intake of carbonated beverages, packaged juices and tea by the participants was associated with poor anthropometric measurements. Contrary to the ...
Alptug Sari*, Ahmet Arpacik and Sagdan Baskaya
...no remarkable effort for saving the Erzurum Marshes, was observed.
...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
... prevalence of Taenia saginata and Hymenolepis nana (tapeworms) infections among farmers, education concerned and shepherds of Swat, Pakistan. A total of 1041 stool samples were examined from January 2006 to December 2008 using direct smear and concentration methods. Two hundred and twenty one (21.2%) participants were found infected with one or more than one intestinal tapeworms. Seventy seven (7.39%) of the p...
Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1
...dead embryos, as well as samples of eggs to microbial analysis were. After 17.5 days of incubation its results were evaluated. Chicks were reared for 14 days and their livability and body weight gains were registered. The effectiveness of grapefruit juice as a disinfectant for hatching eggs was noticed in terms of reducing the development of microflora on the eggshell. Its use did not directly affect the hatching results and chick quality, however, they were s...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...lower by using cheap and safe techniques and making it stable for long period of time in food products as well as in extracted form by using preservatives and antioxidants like citric acid and determining its usefulness by applying on food products. Natural edible color was extracted from T. erecta gave lemon yellow color shade which when applied on candies increased the attractiveness. The spectrophotometric analysis at 474nm of extracted color both in...

Noreen Rasul1*, Madeeha Rashid2, Aqeela Abbas1 and Rubina Sohail

...were 52.2% (3372) and caesarean sections were 47.7% (3087). In 55.4%neonate’s(both preterm and term)early essential newborn care was practiced.Total preterm were 290 (4.5%), out of them 121 (2%) neonates were kept in Kangaroo Mother Care position and 129 (44%) were shifted to neonatal intensive care unit. 14% parents refused for KMC position and discharged. Mortality in preterm newborn in neonatal unit was 29.4% (without KMC) but no mortality occurred af...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...ted. Casein hydrolytic assay identified EB3, EB4, EB6, EB7, EB9, EB11, EB13, UV2, UV4, UV6, UV7, UV9, UV11, UV12 and UV 15 mutants producing halo zones. Percentage blood clot lysis led to screening of mutants UV 2, UV 6, UV 7, UV 9, UV 11, UV 12, UV 15 and EB 3, EB 4, EB 11, EB 13 with hyperactivity as compared to wild bacterial strain. Out of the total 15 isolates of EtBr-treated strains, EB4 gave the maximum enzyme activity (7.3 ± 0.005 U ml-1
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...s isolated from clinical samples. In the current study total 794 patients were tested for suspected tuberculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis complex (MTBC) isolates were randomly selected ...
Ali Murad Rahoo,1,2,* Tariq Mukhtar,1,3 Shoukat Ibrahim Abro,4 Barkat Ali Bughio5 and Rehana Kanwal Rahoo6
... S. kraussei, S. carpocapsae, Heterorhabditis bacteriophora and H. indica) were compared in Galleria mellonella larvae. The production of infective juveniles (IJ) in G. mellonella was significantly affected by nematode species. Significantly higher numbers of IJ were produced by Heterorhabditid species than Steinernematid species in the cadaver. The production of IJ was the maximum in the case of H. bacteriophora which was not s...

Rahila Nizami1, Muhammad Zahid Latif2*, Intzar Hussain3 and Khalid Rashid

...n-probability convenient sampling was conducted involving 59 medical professionals of Azra Naheed Medical College, Lahore. The assessment of leadership-style (T-P leadership rating scale) was applied. The age of subjects was 37.42±11.91 years; 29(49.15%) were males and 27(45.76%) were femalse whereas 3(5.1%) were undisclosed gender. The mean age of males was 41.28±12.85 years and average age of females was 32.83±9.03 years. A total of 36(6...

Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar

...y purposive technique of sampling was used. Diagnostic laparoscopy was conducted to make a diagnosis of endometriosis. Females were divided into two groups. Females in group A received treatment with Letrozole tablets (2.5 mg/day) and in group B received Danazol tablets (600 mg/day) for 3 months. Pelvic pain was assessed using VAS score and the mean decrease in pain score was assessed between two groups using independent sam...

Abida Sajid* and Aqsam Sajid 

...th weight and rate of caesarean section in young Primigravida. It was a cross sectional study, conducted in the department of Gynae & Obstetric unit 5 King Edward Medical University Lahore during a period of one year from May2013 April 2014. All primigravida young 15-24years with anemia enrolled for the study. Anemia is defined as hemoglobin<10.5g/dl (WHO Criteria), Iron deficiency Anemia is defined as serum ferritin level less than 12 micro gram per ml...

Muhammad Naeem Safdar, Khalid Naseem, Muhammad Amjad, Amer Mumtaz and Saeeda Raza*

... and seventy wheat grain samples were collected from nine different regions of Punjab and analyzed for physicochemical characteristics during 2006 to 2008. The quality parameters studied were test weight, 1000 kernel weight, foreign matter (non-edible and edible), broken/shrunken grains, damaged grains, moisture, ash, protein (N x 5.7), wet and dry gluten, falling number, and minerals (copper, manganese, iron, zinc). It was observed that wheat

Raania Ahsan* and Zafar Altaf**

...se varieties employed Monsanto Cry1Acgene (MON 531) as it was not patented in Pakistan. A positive development was approval of Monsanto plan to introduce advanced genetically modified (GM) crop technology in Pakistan by Government of Pakistan during 2009. In this regard Ministry of Food and Agriculture (MINFA) has been working on a two pronged strategy i.e. developing the technology through indigenous capabilities as well as...

Ambreen Akhtar Saddozai and Samina Khalil*

...b-sector markets). These samples were analyzed for total plate count (TPC), total coliforms, fecal coliforms, E. coli, yeasts and moulds. None of the sample was found contaminated with E. coli, contamination of moulds was also negligible. TPC ranged from 1.0x102 to 1.0x104 cfu g-1 except in three cases, whereas total coliform ranged from 0 to 93 except in two cases, where yeast was 0-1600 g-1 and mould upto 250 g-1. All thes...

Golam Moula* and Sayedur Rahman**

...inated seedlings is necessary to explore its viable potentiality at the field level.

...

Zaheer Ahmad, M. Yaqub Mujahid, M. Anwar Khan, Shaheena Yasmin, M. Asif and Maqsood Qamar*

...meeting held at AARI, Faisalabad. The variety case was presented in Punjab Seed Council meeting and was approved as a commercial variety namely NARC-2009 for cultivation in rainfed areas of Pakistan.

...

Sidra Majeed, Shahid Ahmad*, Asif Ali Bhatti** and Ghazanfar Abbas***

...ical fertilizers for the same period were obtained for wheat productivity from the Farm Operations and Services (FOS) crop registers. Water productivity of barani organic desi wheat (C591) grown at NARC was computed using the data of last 10 years (1998-08), which revealed variation from 1.98 to 0.29 kgm-3 of seasonal rainfall. The correlation between seasonal rainfall and water productivity is very high (0.93). There is a strong correlation between seasonal r...

Khalid Mahmood Aujla, Sajida Taj*, Khalid Mahmood** and Nadeem Akmal*

...arch Council through its satellite institutes in the provinces. Total 238 wheat growers were purposively selected from different cropping zones of the Punjab province as sample of the national survey. The survey results reveal that decline in wheat area, the incidence of frost/cold spells, shortage of irrigation water over normal supplies, imbalanced use of fertilizers particularly of DAP fertilizer below the recommended dos...

 Muhammad Asim, Muhammad Asif, Muhammad Munir* and Muhammad Aslam**

... than NODI-3. The phloem sap of Williams-82 had higher accumulation of 3H-ABA at all stages after treatment. The basal levels of ABA in phloem sap were lower in NODI- 3 than in Williams-82. Inoculation further accentuated this difference. The major changes in ABA level in phloem sap and leaves occurred between 48 to 96 h after inoculation.

...

 Aneela Afzal, Tanvir Ali, Munir Ahmad, Humera Amin and Samina Saki*

...tan. A multistage random sampling technique was used to collect the data from 480 respondents from three tehsils of the district. The results show that a vast majority of women were illiterate, having annual family income less than one lac, living in joint family system and having small land farms. A large proportion of the respondents reported that they remained engaged in cleaning of seeds, sowing, harvesting, weeding, etc. for corn, rice, wheat, sugarcane, ...

Hassnain Shah, Hina Riaz, Nadeem Akmal , Muhammad Sharif* and Abdul Majid**

...ted for investment appraisal of the feed mills. Assessment of the intervention based on the farmers’ perception regarding the acceptability and compatibility of the balance feed was done through survey of the participating and fellow farmers at the project sites. Paired sample ‘T’ test was used to compare the difference in milk yield. Farmers reported 0.5 to 2 liters per day increase in milk yield with the ...

 Babar Hussain*, Muhammad Ashfaq**, Mazher Abbas***, Khalid Mahmood**** and M. Ather Mahmood***

...dy used the monthly wholesale price (Rs. / 40 kg) data from January 1991 to December 2006 of gram, in logarithmic form and empirically estimated the degree of integration in gram markets of Pakistan using co-integration analysis. Co-integration results show that all gram markets are highly co-integrated in the long run. The high degree of market integration observed in this case is consistent with the view that Pakistan’s gram markets are quite competiti...

Liaqat Ali Shahid, M. Azhar Saeed and Nadeem Amjad*

...ustard, groundnut, and sesame are the traditional ones while others such as sunflower and canola are the non-traditional oilseeds. Mechanization is one of the important and efficient tools to enhance the crop yield. It also helps to reduce the labour drudgery and ultimately increased farmers’ prosperity.

...

 Zafar Khokhar*, Imtiaz Hussain**, Badruddin Khokhar* and Muhammad Sohail**

 Badaruddin Khokhar*, Imtiaz Hussain** and Zafar Khokhar*

 Rahmat Ullah Khan, Abdur Rashid*, Mohammad Sohail Khan** and Erdogan Ozturk***

...24% over the control and saved 100% cost of the chemical fertilizer. Results suggested that HA applied alone at 3 kg ha-1 or in combination with half (30:20 kg ha-1) rate of NP fertilizers appeared to be the most economical rate to obtain the maximum yield of wheat under the rainfed conditions of Dera Ismail Khan. HA has great potential as a low cost natural fertilizer to improve soil fertility on sustainable basis.

...

Aslam Memon, A. M. Khushk* and Umer Farooq**

...te into the potential of saving land and water resources by re-allocating sugarcane area to relatively more productive recommended sugarcane varieties. Secondly, this exercise shall also provide feedback to researchers in sugarcane crop as well as sugar mills for assessing the types of varieties performing better, relative to others. The study document the extent of adoption of recommended sugarcane varieties in Pakistan. The study was based on the primary dat...

 Mazher Abbas*, Muhammad Waqas Akram**, Ikram Saeed*** and Arshed Bashir*

...dents were taken from Faisalabad, Toba Tek Singh and Chiniot districts of Central Punjab. Percent area allocated to canola crop was comparatively higher at medium farms as compared to small and large farms. The results of the study showed higher yield of canola at large farms as compared to medium and small farms. Average gross revenue by small, medium and large farmers was Rs. 35913.10, Rs. 39635.54 and Rs. 39868.80 acre-1 respectively. Average of net income ...

 Nadeem Akmal, Hassnain Shah*, Muhammad Azam Niazi** and Waqar Akhtar*

...ta were collected from a sample of 38 buck holders and 31 beneficiary farmers using a structured pretested questionnaire. The main influencing factor in keeping bucks was goat breed improvement. All the sample respondents were convinced of the benefits of crosses with Beetal buck and reported that the offsprings Beetal were of higher body weight (40% higher), good looking and well built. Regarding the suitability of Beetal w...

 Muhammad Azam Niazi*, Hassnain Shah, Waqar Akhtar and Nadeem Akmal**

... study period but at the same time overall purchasing power of the farmers dropped. Pakistani farmers are expected to loose and consumers to gain if free agricultural trade (in selected commodities) opened with the neighboring India. It is suggested that farmer friendly policies and cost effective technologies should be transferred to farmers to make agriculture an attractive investment domestically and competitive internationally.

...

 Ahmed Aziz Kurd, Amanullah, Saifullah Khan, Basharat Hussain Shah and Munir Ahmed Khetran*

... Quetta, Pakistan. The basal portion of these cuttings were treated with 3000, 4000 and 5000 ppm IBA solution for 3- 4 seconds. The treated cuttings were immediately planted in polyethylene tubes (10 cm x 20 cm) filled with a sandy loam soil. In the control, the cuttings were planted directly in polyethylene bags without IBA application. Plants were kept in a shade house and humidity was increased manually by hand sprinkling...

 Ghulam Shabbir*, Muhammad Aftab, Abid Mahmood** Muhammad Kausar Nawaz Shah* and Nasir Mahmood Cheema***

 Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan*

...(Ocimum basilicum and O. sanctum) were collected from different agro-ecological zones of the world and compared for their phenotypic (qualitative and quantitative) and biochemical trait. Comparatively high variation was seen for seed germination, canopy and spikes/plant, florets/spike and leaf area. A UPGMA cluster, grouped the 9 Ocimum genotypes into two major clusters on the basis of total seed protein and phenotypic characters, except Siam Queen and Lime ba...

 Zaheeruddin Mirani*and Aslam Memon**

...ctive in transferring messages, however, they were limited to their product sales since they have the task to achieve targets rather farmers’ development. Agricultural suppliers/ dealers of the pesticide/fertilizer product sales were the most influential persons of the area as indicated by the farmers. Agricultural supplier/dealer makes all the decisions of the farmers regarding the ...

 Muhammad Azeem Khan*, Abid Hussain, Irfan Mehmood and Sonila Hassan **

...r production and vice-versa. Farmers perceived a major change in climate at Kohat and a minor change at Attock over time. They reported unsustainability in production practices at Kohat and water management practices at both sites. Main strategies adopted by the farmers to mitigate the effects of climate change were occasional sale of livestock, reduction in consumption expenditures, and migration to nearby cities for non-fa...

 Rubina Bano, Hassnain Shah, Muhammad Sharif and Waqar Akhtar*

...c characteristics of the sample poultry farmers along with cost and profitability analysis. Broiler farming was done by adult males on full time basis. It was the main source of income of a family. The average flock size in the study area was 4033 birds. The study also bifurcates the cost structure and fixed cost accounts for 7 % while variable cost accounts for 93% of total cost of production. Feed makes the major share and accounts for 49.34%. Broiler produc...

 Muhammad Afzal, Sarfraz Ahmad , Abdul Salam Baloch* and Qazi Bashir Ahmad**

...ficients of variation. A sample of price observations of 725 animals (389 sheep and 336 goats) from Quetta livestock market was collected for one year on quarterly basis with a minimum sample of 150 animals. The objective was to determine seasonal price variation considering the effects of different attributes of the animals namely, live weight, age, gender, body score and time of sale of ...

Muhammad Athar Khan1, Muhammad Zahid Latif2*, Syed Amir Gilani3 and Ifrah Bukhari4 

...y technique was used for sampling. In the first stage one union council was selected from each district randomly. In the second stage, 25 subjects involved in rice cultivation from the last 10 years were selected from each union council. Similarly 25 subjects who never worked in the rice paddy field were also randomly selected from each union council as controls. The serum sample of each subject was tested against each of th...

Mian Sajjad Ahmad1, Somia Iqtadar1, Sami Ullah Mumtaz1*, Zafar Niaz1, Iqra Waheed2 and Sajid Abaidullah

...on-probability purposive sampling. Patients aged 15-70 years of either gender were included. Oral glucose tolerance (OGTT) was done in each patient with unrestricted carbohydrate diet for three days and avoiding coffee, smoking and heavy exercise six hours before the test. Fasting sample was taken then 75g oral glucose was given. Sample after two hours was sent to KEMU clinical lab. A positive result of the patient was label...

Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1 

... one of the common and disabling causes of headaches, has been described by World Health Organization as close to quadriplegia and dementia.Among the headache complaint visitors in the emergency department, the most common are those suffering from migraine. (1) Alinear correlation has been found between rising serum pro BNP levels and Cardiovascular pathologies especially those involving left ventricular dysfunction which may depict a probable association betw...

Halima Shoukat1*, Hafiz Sheraz Arshad2, Faiza Sharif1, Arooj Fatima1 and Filza Shoukat

...nt was measured by universal goniometer before the commencement of stretching technique. Static stretching was applied to group A, B and C with 30, 60 and 90 seconds holds, respectively. Stretches were exerted at frequency of three sessions per week and three repetitions per session. Reevaluation of ROM was performed after 4 and 6 weeks. The mean values of ROM of knee extension showed gain in range after applying static stretching at 30s, 60s and 90s holding t...

Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2* 

...this study, canine fecal samples from diverse States of Brazil were screened by PCRs for CCoV and positive samples were subjected to partial sequencing for the membrane (M), spike (S), nucleocapsid (N) and non-structural protein 3b genes. Out of the samples collected, 40.17% were positive for CCoV; 57.45% of CCoV-infected animals showed enteritis and most of these (76%) were younger than 3...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...oncept that due to mass usage of live IBV vaccinal strains of diverse origin, variety of variants originate through random spontaneous mutation and genetic recombination which could lead to genetic drift. The emergent strain of IBV in this study points out the need to include such variants in killed-vaccine form in the vaccination program of the affected region.
...
Nay Naing Htoo1, Basit Zeshan1,2,*, Aung Tun Khaing1, Than Kyaw1, Erkihun Aklilu Woldegiorgis1 and Mohd Azam Khan1
...ach treatment group were sacrificed. The results showed that the rumen morphological characteristics, papillary surface area and keratinized layer of CFR (4.15mm2, 50.54µm), were not significantly different from CF (3.81mm2, 49.26µm) (P>0.05) but it was significantly different from kids in DM group (1.17mm2, 26.36µm) (P<0.05). The rumen papillary surface area and keratinized layer were also significantly...
Abul Hassan Faiz*, Fakhar-i-Abbas and Lariab Zahra Faiz
... in eight types of soil (sand, loamy sand, sandy loam, sandy clay loam, loam, clay loam, silt loam, silty clay loam) to determine six soil properties (plant biomass, saturation, electrical conductivity, pH, organic matter, phosphorus and potassium) in Pothwar Plateau (250 km long and 100 km wide). We compared 216 soil ...
Amtul Jamil Sami1,*, Sehrish Bilal1, Madeeha Khalid1, Muhammad Tahir Nazir1 and A.R. Shakoori2

 

... describes the effect of saponins, isolated from a medicinal plant Azadirachta indica on the CNS enzymes of a stored grain pest, Tribolium castaneum and a socioeconomic insect, Apis mellifera. A comparative study was designed to identify the role of saponins on insect acetylcholinesterase (AChEs). The enzyme activities were tested for the effect of saponins. The AChE a...
Nazish Shaukat1, Muhammad Javed1,Faiza Ambreen2* and Fariha Latif1
... After 30 days, fish was sacrificed and their gills and liver isolated to determine the effect of PbCl2 on peroxidase activity. Activity of peroxidase enzyme in metal stressed fish was compared with control. The physico-chemical parameters of the test media viz. pH, dissolved oxygen, carbon dioxide, total hardness, calcium, magnesium and total ammonia were monitored on daily basis. Significantly increased peroxidase activity was observed in g...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 tributaries (YX and TB population) of Hanjiang river. A total of 59 alleles were found and the number of alleles in each locus ranged from 3 to 8, with an average of 5.4. There were 6 highly polymorphic loci and 4 moderately polymorphic loci. ...

Muhammad Zafarullah Khan1*, Rehmat Ullah1, Asif Nawaz1 and Ejaz Ashraf2 

... Khan and Swabi were the sampled districts and 80 farmers registered with Farm Services were randomly selected from each district. Thus, sample size was making a total of 400 respondents. Primary data were collected with the help of pre-tested interview schedule through personal interview method. The results of this research study revealed that affinity toward Farm Services Centers were bulk of the educated, middle aged and ...

Anish Shrestha 

...randomly selected as the sample for the study. Farmers were rearing on an average 34.54 hives and average honey productivity was 34.6 Kg/hive. Average production cost was NRs. 7392.52 with the average net profit of NRs. 2987.05 (1 USD = 106 NRs), and B:C ratio was 1.67. Labor cost, migration cost and expenditure on sugar drug and comb foundation seems to have positive and significant relation with gross return. All of them appeared to be underutilized and need...
Asad Sultan1, Sahal Ahmad1, Sarzamin Khan1, Rifat Ullah Khan2,*, Naila Chand1, Muhammad Tahir3 and Shakoor Ahmad2
...up of birds received a basal diet only (control group), second group of birds received a diet supplemented with silymarin at the level of 10g/kg and third group of birds received a diet supplemented with ZnO at the level of 60 mg/kg in both TN and HS birds. It was found that supplementation of ZnO significantly (P<0.05) improved feed intake, dressing percentage, body weight, feed conversion ratio, blood parameters and nutrient digestibility at both levels o...
Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and  John E. Foster6
...erorhabditid nematodes assayed against these ticks using the Petri dish assay. Concentrations of 0, 500, 1000, 2000, 4000 and 8000 IJs were applied in 1 mL of sterile distilled water dispensed on the surface of a couple of moistened filter papers. For each treatment concentration, 10 adult engorged ticks were placed in the Petri dishes, and each treatment was replicated four times; the experiment was replicated two times. Ti...
Umair Riaz*1, Zafar Abbas1, Qamar uz Zaman2, Muhammad Mubashir1, Mahwish Jabeen2, Syed Ali Zulqadar1, Zeenat Javeed1, Saeed-ur-Rehman1, Muhammad Ashraf1 and Muhammad Javid Qamar1
...or irrigation use. Water samples from different areas of the tehsil were collected and analysed for various physic-chemical properties like pH, electrical conductivity (EC), sodium adsorption ratio (SAR), residual sodium carbonate (RSC), calcium (Ca++), magnesium (Mg++), sulfates (SO4-), carbonates (CO3-), bicarbonates (HCO<...
Naveed Akhtar,1,* Riaz Muhammad2, Kausar Saeed2, Muhammad Fiaz Khan1, Muzaffar Shah3, Jehan Zeb2, Shahid Ahmad2, Azam Jan Afridi4 and  Aftab Hussain5
...h problems to ensure the safety of mammalian fauna of Malka and Mahaban valley district Buner.
...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...rasferase, aspartate transaminase and blood urea nitrogen as compared to isoflurane anesthesia; whereas, no statistical difference (P>0.05) was observed for alkaline phosphatase, creatinine and bilirubin between the two groups. Heart rate, blood pressure and saturation of oxygen were decreased in both groups but this decline was significantly lesser (P<0.05) in isoflurane administered group compared to xylazine-ketamin...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
...cleotide sequence of the sample MR02 was found completely homologous with the Pre-S2 region of already reported subtype “adr4”. This sample showed 1.2 to 20% variation with the other reported sequences. Nucleotide sequence of the Pre-S2 region of the second sample MR03 does not show 100% homology with any of the reported sequences. It differs 2.4 to `18.8 % from the other repor...
Lin Wang1,2, Ye Gong3, Kelin Chen1, Haitao Wang3 and Xianguo Lyu1,*
...amplification of 90 microsatellite loci developed for eight other species. Eleven out of them were successfully amplified and polymorphic with 2-10 alleles. The observed heterozygosities ranged from 0.481 to 0.827 and the polymorphic information content ranged from 0.373 to 0.775. Significant linkage disequilibrium was found only between two markers. These microsatellites could be used to enhance our understanding of genetic...
Qin Liu1,2, Feiwei Liu1, Xiuyue Zhang1, Nan Yang1 and Jianghong Ran1,*
...hic tetranucleotide microsatellite markers were isolated from T. szechenyii using a next-generation sequencing technology. The allele number of these loci ranged from two to six in genotyped 35 individuals. Polymorphism information content ranged from 0.2735 to 0.7717 with an average of 0.5149. Observed and expected heterozygosities at each locus ranged from 0 to 0.879 and 0.332 to 0.814, respectively. These markers could be used to better understand th...

Imtiaz Hussain*, Hassnain Shah, M. Azeem Khan, Waqar Akhtar**, Abdul Majid*** and M. Yaqub Mujahid*

Corresponding author:izhussain@yahoo.com

PRODUCTIVITY IN RICE-WHEAT CROP ROTATION OF PUNJAB: AN APPLICATION OF TYPICAL FARM METHODOLOGY
...through purposive random sampling applying typical farm methodology. Rice is cultivated on 87% and fodder on 8% of the total area during kharif while wheat on 75% along with berseem on 14% of total area during rabi season. Almost 81% rice and more than 50 % wheat is harvested with combine followed by burning of residues that not only create environmental pollution but also affect the productivity of the cropping system. Stagnation in yield of rice and wheat is...

Akhlaq Ahmad, M. Anwar Arain*, Rahila Nazli**, Syed Anser Rizvi***,
Mubarik Ahmed* and Farzana Ibrahim****

Corresponding author: dr.akhlaqahmad@yahoo.com

COST EFFECTIVENESS OF UNDER-SHEET AND WHOLE GODOWN FUMIGATION OF BAGGED WHEAT

 Nazakat Nawaz, Malik Shah Nawaz*, Nasir M. Cheema** and Mubashir A. Khan*

Corresponding author:nazakat_nawaz@yahoo.com

ZINC AND IRON APPLICATION TO OPTIMIZE SEED YIELD OF MUSTARD
...study was conducted on a sandy clay loam soil at the National Agricultural Research Centre, Islamabad, Pakistan, during 2005 and 2006. The mustard (Brassica juncea) variety BARD-1 was treated with various -1 levels of Zn and Fe, (0-0, 0-1.5, 0-3, 2.5-0, 2.5-1.5, 2.5-3, 5-0, 5-1.5 kgha and -1 -1 5-3 kgha ), respectively. A basal dose of 90N and 60P kg ha was applied, in the form of Urea and triple super phosphate (TSP) with Z...

 Hidayatullah, T. Mahmood, M. Farooq, M. A. Khokhar and S.I. Hussain*

* Corresponding author: hidayatu2003@yahoo.uk.co.uk

PLANT GROWTH REGULATORS AFFECTING SEX EXPRESSION OF BOTTLE GOURD (LAGENARIA SICERARIA MOLINA) PLANTS
...ia Molina) plants cv. Faisalabad Round was investigated under field conditions in National Agricultural Research Centre, Islamabad. Plants sprayed with distilled water were considered as control. Among all foliar agents, the response of -l GA3 3 and MH was found better. Exogenous application with 30 µmol l GA maximally increased the pistillate flower production as compared to control. Moreover, the treatment produced maximum number of fruits -1 -1 (22.24...

  Farhat Ullah Khan, Nowshad Khan and Zaibunnisa Abdullah*

Corresponding author: farhatkhan7@gmail.com (This is part of Ph.D. work of senior author)

IMPACT OFKINNOW (MANDARIN) YIELD LOSSES ON SOCIOECONOMIC CONDITIONS OF THE FARMING COMMUNITY IN PUNJAB, PAKISTAN

 Hassnain Shah, Muhammad Azeem Khan, Umar Farooq, Nadeem Akmal*, Shahid Munir and Bashir Hussain**

POTENTIAL FOR INVESTMENT IN INDIGENOUS TECHNOLOGIES: A CASE OF LOW COST SOIL AND WATER CONSERVATION STRUCTURES IN RAINFED POTHWAR, PAKISTAN
...ea. The investment appraisal based on the cost incurred and benefits realized by the farmers recorded through assessment survey revealed that the technology had a short payback period along with internal rate of return varying in the range of 25-37% under rainfed conditions and positive net present value. The farmers' perceptions revealed that the technology can solve the erosion problem along with 10-35% increase in crop yield and sa<...

 Faisal Khalil, Khalid Mahmood Qureshi, Ammaz Khan, Fakhar-ul-Hassan and Nabila Bibi*

EFFECT OF GIRDLING AND PLANT GROWTH REGULATORS ON PRODUCTIVITY IN OLIVE (OLEA EUROPAEA)
... gibberellic acid (GA ), salicylic acid (SA) and naphthalene acetic acid (NAA) alone and/ 3 or coupled with girdling operations may improve cropping potential of existing olive varieties in Pakistan. Therefore, this study was envisaged on olive trees to explore possibilities of inducing fruitfulness through the use of hormones and girdling operations und...

 Khalid Mahmood Qureshi, Fakhar ul Hassan, Qamar ul Hassan, Usman Shaukat Qureshi, Saman Chughtai and Adnan Saleem*

IMPACT OF CULTIVATION SYSTEMS ON GROWTH AND YIELD OF STRAWBERRY (FRAGARIA ANANASSA) CV. CHANDLER
...rawberry (Fragaria ananassa) cv. Chandler were collected from Swat (Mingora.) Different cultivation systems were adapted i.e., standard growing media in polyethylene bags (T ), soil less media (T ), high tunnel (T ), green house (T ), 1 2 3 4 including open field condition on ridges (T ) by keeping plant to plant 5 distance of 30 cm and row to row distance of 60 cm. The study was conducted to evaluate the effects and suitability of different cultivation system...

 Muhammad Tariq*, Raja Muhammad Omer**, Muhammad Ashraf Mian*, Obaid Ur Rehman***, Amjad Tahir Virk and Kazim Abbass**

PROMOTING CERTIFIED SEED AVAILABILITY OF WHEAT (TRITICUM AESTIVUM L) THROUGH PUBLIC-PRIVATE PARTNERSHIP AND ITS IMPACT ON YIELD IN RAINFED AREAS
...nomic practices were the same in both -2 treatments. The number of fertile tillers m were significantly higher in certified seed source than the farmer's own seed that resulted in significant increase in grain yield. Other yield contributing parameters including number of spikelet per spike, numbers of grains per spike and 1000 grain weight were at par in both seed sources. It was concluded that healthy and pure seed source gave high seed germination and good ...

 Ashiq Saleem, Muhammad Ashraf Zahid, Habib Iqbal Javed*, M Ansar**, Asghar Ali, Rashid Saleem* and Noor Saleem***

EFFECT OF SEEDING RATE ON LENTIL (LENS CULINARIS MEDIK) SEED YIELD UNDER RAINFED CONDITIONS
...ainfed conditions can be safely recommended.

...

 Gulzar S. Sanghera and Wassem Hussain*

HETEROSIS IN RELATION TO COMBINING ABILITY PER SE PERFORMANCE IN TEMPERATE RICE (ORYZA SATIVA L.)
...omponents of rice (Oryza sativa L.). Analysis of variance revealed significant differences among genotypes, crosses, lines, testers and line x tester interactions for all the traits studied. The specific combining ability (SCA) effects along with per se performance revealed that some of the crosses have shown desirable SCA effects, having superior per se performance for most of the traits thus indicating the selection of these crosses. Based on standard check ...

 Safdar Ali, Sahiba, M. Azim Malik, Fayyaz-ul-Hassan and M. Ansar*

GROWTH OF RAINFED FODDER MAIZE UNDER DIFFERENT LEVELS OF NITROGEN AND PHOSPHORUS
...-1 organic matter 0.65%, saturation 30%, available phosphorus 5.7 mg kg -1 and available potassium 78 mg kg . Maize cv. Sargodha-2002 was sown in randomized complete block design (factorial) and was fertilized with four -1 each nitrogen @ 0, 60, 120 and 180 kg ha and phosphorus 0, 30, 60 and 90 -1 kg ha doses. Significant increase in green fodder and dry matter yield was observed with the increased doses of nitrogen and phosphorus. Overall, the -1 nitrogen app...

 Sonila Hassan, Abid Hussain*, Muhammad Azeem Khan** and Irfan Mahmood*

RURAL-URBAN RETAIL PRICES AND MARKETING MARGINS OF FRESH FRUITS AND VEGETABLES IN PAKISTAN
...tailers in Hyderabad, Faisalabad, Peshawar, Muzaffarabad and Quetta. It was observed that the retail prices of fresh fruits and vegetables were higher in rural markets as compared to urban markets and differences were even higher in vegetables as compared to fruits. For fruits the difference ranged from 8.6-25.0% in Hyderabad, 5.2- 39.0% in Faisalabad, 2.3-22.0% in Muzaffarabad, 20.0-50.0% in Peshawar and 11.0% in Quetta. Di...

 Ambreen Akhtar Saddozai, Saeeda Raza* and Shahzad Arshad Saleem**

MICROBIAL COUNT AND SHELF LIFE OF STRAWBERRY JUICE
...re increased with the passage of time. Spoilage can be controlled by keeping the juice in refrigerator and using preservatives. Thus with good storage conditions, the shelf life of the strawberry juice can be increased satisfactorily.

...

 Saima Rani and Irum Raza*

COMPARISON OF TREND ANALYSIS AND DOUBLE EXPONENTIAL SMOOTHING METHODS FOR PRICE ESTIMATION OF MAJOR PULSES IN PAKISTAN
... growth rates remain the same then prices of these pulses in Pakistan would be Rs.37.64, Rs.120.26, Rs.89.55 and Rs.55.03 per kg, respectively in 2015- 16. An increasing trend in the estimated prices will turn down the demand of these pulses and consequently poor class of the economy who do not have enough resources to buy expensive livestock-based protein-rich food will be badly affected.

...

 Akhtar A. Siddiqui and Allah W. Rind*

EXTENSION FIELD WORKERS PERCEPTION OF COTTON INTEGRATED PEST MANAGEMENT PROGRAMME IN SINDH PROVINCE
...ri, and Mirpurkhas). The sample size comprised 48 EFWs who participated in Training of Facilitators (ToF) and erecuted FFSs during 2001 and 2004. The results revealed that the EFWs performed effectively to attain the objectives of IPM programme. It appears that EFWs improved farmers' knowledge, skills and behavioral change in attitude towards agro-ecological sound IPM practices through FFS training. 

...

 Arshad Ali, Muhammad Arshadullah, Syed Ishtiaq Hyder, Imdad Ali Mahmood and Badar-uz-Zaman*

RICE PRODUCTIVITY AND SOIL HEALTH AS AFFECTED BY WHEAT RESIDUE INCORPORATION ALONG WITH NITROGEN STARTER DOSE UNDER SALT-AFFECTED SOIL
...strict Sheikhupura under saline sodic -1 soil (ECe=5.65 dS m , pH=8.57and SAR=19.38) during 2010. Treatments were arranged in RCBD with three replications. The crop was harvested at maturity, data on tillering, plant height, spike length, number of grains -1 spike , 1000-grain weight, straw and paddy yields were recorded. Plant samples collected at matur...

 Syed Ishtiaq Hyder, Muhammad Arshadullah, Arshad Ali and Imdad Ali Mahmood*

EFFECT OF BORON NUTRITION ON PADDY YIELD UNDER SALINESODIC SOILS
... on raised beds under -1 saline sodic soils (ECe=5.32 dS m , pH=8.52 and SAR=18.87) at Soil Salinity Research Institute, Pindi Bhattian, district Hafizabad, Punjab Pakistan during 2009 and 2010. Treatments were arranged in RCBD with three replications. The crop was harvested at maturity. Data on tillering, -1 plant height, spike length, number of grains spike , 1000-grain weight, straw and...

 Munir Ahmad* and Asif Ali Mirani**

HEATED AIR DRYING OF GROUNDNUT
...ible to dry it down to a safe moisture level by sun drying. Therefore, the chance for developing aflatoxins in it is high. To solve this problem of the groundnut growers, a low cost mobile flat-bed dryer designed and developed at Agricultural and Biological Engineering Institute (ABEI), National Agricultural Research Centre, Islamabad was evaluated for drying groundnut. The dryer had been found capable to dry about 1818 kg groundnut from 23.3% moisture content...

 Muhammad Zubair Anwar, M. Azeem Khan, Ikram Saeed, Akhtar Ali*, Shafique Zahid and Abdul Majid**

SMALL FARMERS PERCEPTIONS REGARDING IMPROVED FODDER AND FORAGE VARIETIES: RESULTS OF PARTICIPATORY ON FARM RESEARCH
...ld was increased and 44% said that the increase in dry stalk was about 50%. While in kharif 72% respondents pointed out 55% increase in grain yield and similarly majority of the farmers (68%) pointed out 50% increase in dry stalk yield. Majority of the respondents (83%) appreciated the newly introduced plant species while about 17% have shown their interest in fruit trees.

...

 A.A. Mengal* , M. U. Mallah, Z. A. Mirani* and B. N. Siddiqui**

AN ANALYSIS OF PUBLIC AND PRIVATE AGRICULTURAL EXTENSION SERVICES IN BALOCHISTAN, PAKISTAN
...ng, Sibi, and Loralai. A sample of 375 farmer respondents were taken with a systematic sampling procedure. The response rate was 82.67%. A detailed and comprehensive questionnaire was used for measuring the effectiveness of extension activities performed by public and private sector extension field staff. The results revealed that a number of farmers received visits from private extension field staff on a fortnightly, monthl...

 Arshad Ali, Imdad Ali Mahmood, Muhammad Salim, Muhammad Arshadullah* and Abdul Rasool Naseem**

GROWTH AND YIELD OF DIFFERENT BRASSICA GENOTYPES UNDER SALINE SODIC CONDITIONS
...as conducted at farmer's salt-affected field -1 (ECe=12.3 dS m ; pH=9.7; SAR=46.2) in Hafizabad to test growth and yield response of six Brassica cultivars (BARD-I, Dunkled, Rainbow, BRS-II, Sultan Raya and cv. 95102-5) under saline sodic conditions. Data on growth and yield parameters were collected randomly (average of five plants per replication) at t...

 Sajida Taj*, Muhammad Tariq Iqbal Khan**, Mazher Abbas and Arshed Bashir***

PRICE SPREAD AND MARKETING MARGINS OF CUT ROSE IN PUNJAB, PAKISTAN
...from 34 farmers, 13 wholesalers-cum-commission agents and 20 retailers from main flower producing areas of Kasur and Faisalabad districts from Punjab province, the structure and marketing margins of producers and other marketing intermediaries are quantified along the existing two marketing channels for cut flowers. In the first channel the main players were producers, wholesalers-cum-comm...

 Ayesha Tahir* and Zafar Altaf**

DETERMINANTS OF INCOME FROM VEGETABLES PRODUCTION: A COMPARATIVE STUDY OF NORMAL AND OFF-SEASON VEGETABLES IN ABBOTTABAD
...bles in Pakistan is necessary not only for increasing farmer's income and production diversity concerns, but also for increasing the intake of micronutrients in human diet through increased vegetable consumption. The spectrum of year round availability of vegetables could be further extended. The off-season vegetable production offers a promising opportunity in this direction. The study is based on comparison of income obtained from production both in normal a...

 Amir Khatam*, Sher Muhammad, and Ijaz Ashraf**

ROLE OF INDIVIDUAL CONTACT METHODS IN DISSEMINATION OF AGRICULTURAL TECHNOLOGIES
...akhtunkhwa including Charsadda, Hangu, Kohat, Mardan, Nowshera, Peshawar and Swabi were selected. Data were collected on various aspects of individual contact methods in diffusion of agricultural technologies from 280 randomly selected farmer respondents. Researchers collected the data with the help of a pretested and validated interview schedule using survey technique. The results show that majority of the farmer respondents is aware of the farm visits (66.43...

 Muhammad Arshadullah, Syed Ishtiaq Hyder, Arshad Ali and Imdad Ali Mahmood*

CUMULATIVE EFFECT OF SULFUR AND CALCIUM ON WHEAT GROWTH AND YIELD UNDER SALINE-SODIC SOILS
... variety (Saher) sown in saline-sodic soil (ECe=5.32 dS m , pH=8.52 and SAR=18.87) at Soil Salinity Research Institute (SSRI) Farm, Pindi Bhattian during rabi 2009-10. Treatments were arranged using randomized complete block design (RCBD) with three replications. The crop was harvested at maturity, data on tillering, plant height, spike -1 length, number of grains spike , 1000-grain weight...

 Muhammad Sohail*, Imtiaz Hussain*, Riaz-ud-din*, Syed Haider Abbas*, Maqsood Qamar* and Muhammad Noman*

EFFECT OF SPLIT N FERTILIZER APPLICATION ON PHYSIOAGRONOMIC TRAITS OF WHEAT (TRITICUM AESTIVUM L.) UNDER RAINFED CONDITIONS
...tion methods i.e. full basal N dose at planting and N application in two and three equal split doses at tiller formation and stem elongation stages. Maximum grain yield -1 -1 (5.20 t ha ) was achieved when N was applied @120 kg ha in three equal split doses at planting, tiller formation and stem elongation stages. N application in 2 and 3 split doses resulted in 25 - 50% grain yield advantage at all N rates as compared to single basa

 Sajida Taj*, Akhter Ali*, Nadeem Akmal*, Shujaat Yaqoob** and Mubarik Ali***

RAISED BED TECHNOLOGY FOR WHEAT CROP IN IRRIGATED AREAS OF PUNJAB, PAKISTAN
...a days. Therefore, water saving becomes the utmost need of the hour. The national research system is now putting their focus and efforts to manage the precious water through various modern/latest water saving models to draw some solid method of irrigation with less wastage. Raised bed planting method is also one of the modern methods of planting crop with significant water saving. The stud...

 Waqar Akhtar*, Nadeem Akmal*, Hassnain Shah*, Muhammad Azam Niazi** and Ayesha Tahir*

EXPORT COMPETITIVENESS OF PAKISTANI HORTICULTURAL PRODUCTS
...rative and competitive disadvantage to comparative and competitive advantage during the period under analysis. Tangerines, mandarins, clem have maintained relatively higher revealed comparative advantage as compared to other categories for the whole period under analysis. Onion export has revealed comparative advantage with some fluctuations over time. The research indicates that Pakistan's comparative and competitive advantages have been increasing in all the...

 Muhammad Farooq*, Hidayat Ullah*, Nausherwan Nobel Nawab* and Khalid Mahmood Qureshi**

EVALUATION OF INDIGENOUS TOMATO HYBRIDS UNDER PLASTIC TUNNEL

 Sara Khalid*, Khalid Mahmood Qureshi*, Ishfaq Ahmad Hafiz*, Khalid Saifullah Khan* and Usman Shaukat Qureshi*

EFFECT OF ORGANIC AMENDMENTS ON VEGETATIVE GROWTH, FRUIT AND YIELD QUALITY OF STRAWBERRY
...rawberry (Fragaria ananassa Duch.) cv. Chandler which included T = planting 1 media -1 (soil + silt + farm yard manure); T = planting media + 400 mgl humic 2 -1 acid; T = planting media + 200 g kg leaf manure; T = planting media + 200 3 4 -1 -1 g kg vermicompost; T = planting media + 200 g kg plant fertilizer and T = 5 6 -1 planting media + 200 g kg bio-compost during 2011-12 at PMAS-Arid Agriculture University, Rawalpindi. Treatment T (soil + silt + FYM) indu...

 Rabeea Tariq*, Khalid Mahmood Qureshi*, Imran Hassan*, Muhammad Rasheed* and Usman Shaukat Qureshi*

EFFECT OF PLANTING DENSITY AND GROWING MEDIA ON GROWTH AND YIELD OF STRAWBERRY
...trawberry (Fragaria ananasa), belonging to Rosaceae family, is a rich source of vitamins and minerals with delicate flavors. It is perishable crop which is exceedingly in demand for its taste, profitability, high yield and good quality. To make the plant growth successful in the container, the requirement of special media is very important step because plant growth is largely depended on the physiochemical properties of the ...

 Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**

PRODUCTION AND MARKETING OF CAMEL PRODUCTS IN SEMIDESERT AND DESERT AREAS OF PAKISTAN
...ed through simple random sampling technique in April, 2011 from 220 camel farmers and 17 market intermediaries. It is found that both camel farmers and market intermediaries were less educated. It is observed that markets for camel milk, meat, hides and hair are less established in semi desert and desert areas of the country. Mean production of milk per farm household was 5.4 and 6.5 liters per day in summer and winter seasons, respectively; however, none of t...

 Naheed Zahra*, Muhammad Zubair Anwar*, Sonila Hassan** and Irfan Mehmood**

INSTITUTIONAL CREDIT ARRANGEMENT AND THEIR IMPLICATION ON AGRICULTURAL INCOME IN THE SELECTED VILLAGES OF RAWALPINDI DISTRICT
...ricultural income of the sample farmers. The study was based on the primary data collected from 150 farmers through purposive random sampling. Regression analysis was done to obtain the results. Ordinary Least Square (OLS) method was applied to generate the results. Results of this study revealed that there exists a positive and statistically significant relationship between institutional credit and agricultural income of th...

 Muhammad Yasin Mirza*, Mubashir Ahmad Khan*, Muhammad Amjad* and Malik Shah Nawaz*

STABILITY ANALYSIS FOR ECONOMIC TRAITS IN SESAME ( L.)
... 10 elite genotypes of sesame across three locations for the estimation of genotypes environment(GE) interaction and to identify stable genotype(s) using stability parameters, and to determine inter-parameters correlation. Two locations (NARC and D.I. Khan) were suitable for high productivity. PR-19-9-S produced maximum (341 kgha ) at NARC and V-90005 produced 344 kgha and PARS-I produced 304 kgha at D.I Khan. The variance due to GE interaction was highly sign...

 Muhammad Shahzad*, Akhter Ali**, Abdul Hayee Qureshi**, Naveed Jehan*, Imran Ullah* and Majid Khan*

ASSESSMENT OF POST-HARVEST LOSSES OF PLUM IN SWAT, PAKISTAN
...nel of the plum was necessary for the stake holders to make informed decision about harvest and post harvest practices. This study was conducted in Swat district in 2012 to quantify these losses at different levels using four different questionnaires. Proportionate stratified random sampling technique was used for the collection of data from different categories of respondents and tehsils. Post harvest losses were estimated ...

 Nasia Batool*, Imtiaz Ahmad Qamar**, Imdad Hussain Mirza** and Muhammad Fateh Ullah Khan**

MIXING LESS PALATABLE GRASSES WITH UREA, MOLASSES AND EFFECTIVE MICROORGANISMS AND ITS EFFECT ON CHEMICAL COMPOSITION AND DIGESTIBILITY IN GOATS
...a. Proximate analysis of samples was carried out. Crude protein content of mixtures improved as compared with sole grasses. Digestibility of HC supplemented with urea, molasses and EM in various combinations was also studied in growing goats. The highest digestibility of DM in goats was recorded in HC + 4% urea + 4% molasses treatment (85.51%) followed by HC + 4% urea (78.57%) and HC + 4% urea + 4% molasses + 1:100 EM (78.00%).

...

 Abdul Rehman Roonjho*, Waseem A. Gillani**, Awais Rasool**, Naheed Akhtar**, Tariq Mahmood**, Arsalan A.***, M. Afzal*, Iqbal Khan* , M. Asghar Ranjha**** , M. Irfan**** and Javed Khan**

REPELLENCY EFFECTS OF DIFFERENT PLANT EXTRACTS TO COTTON MEALY BUG, TINSLEY (HEMIPTERA: PSEUDOCOCCIDAE)
...cts of peach plant L. (Rosales: Rosaceae), Eucalyptus, L. (Myrtales: Myrtaceae), Ashok, (Magnoliids: Annonaceae), Milk thistle, (Asterales: Asteraceae), and Sow thistle, (Asterales: Asteraceae) extracts each in petroleum ether, acetone and ethanol were evaluated at the concentration of 1000, 500 and 250 ppm against cotton mealy bug ( ) in a free choice bioassay for two weeks. The ethanol e...

 Shahid Ali*, Anjum Shehzad*, Muhammad Ather Rafi* and Ahmed Zia*

INSECT POLLINATORS OF LITCHI ( ) FROM DISTRICT HARIPUR, PAKISTAN

 Adnan Umair*, Safdar Ali**, Muhammad Sarwar***, Kashif Bashir**, Muhammad Javed Tareen**** and Muhammad Asghar Malik*****

ASSESSMENT OF SOME PRIMING TECHNIQUES IN MUNGBEAN (VIGNA RADIATA) : A GREEN HOUSE STUDY
... and hormonal priming by salicylic acid (10 and 20 ppm). Untreated dry seeds were used as control. All the invigoration treatments significantly improved plant vigor in terms of final germination biomass, root, shoot length and nutrient uptake of mungbean seedlings compared to control (non-primed dry seedling). The application of seed priming also improved the protein concentration at the early stage of seedlings. Phosphorous application through priming signif...

 Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*

COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
... were increased in the basal MS media for culturing the grape plantlets, there was a corresponding increase in the number of shoots and root mass. Additionally, the shoot proliferation response to increasing level of BAP was found genotype dependent. Naphthaleneacetic acid (NAA) also showed a positive effect on in vitro growth responses (shoot proliferation and root induction) when combined with BAP. The media containing NAA, BAP and kinetin accelerated the gr...

 Tabinda Qaiser*, Ayesha Tahir*, Sajida Taj* and Murad Ali*

BENEFIT-COST ANALYSIS OF APICULTURE ENTERPRISE: A CASE STUDY IN DISTRICT CHAKWAL, PAKISTAN
...wed for getting the necessary data to determine the costs and return of beekeeping and assess the scope of beekeeping. The benefit cost ratio of beekeeping was 1.4709 which showed that this business was profitable. It will be a great source of employment creation for the rural poor people to reduce the poverty. There is a great prospect of beekeeping in Pakistan on the basis of the socioeconomic context of the country and some special features of the enterpris...

 Shahida Khalid*and Shahida Nasreen Khokhar*

INTERACTION OF HERBICIDES AND BIO-INOCULANTS WITH AGRICULTURAL CROPS AND WEEDS
...ronment through their versatile role. Whereas weeds are a limitation and herbicide carryover can also be injurious to subsequent crops. The residual/carryover herbicides may influence sowing decisions for the next crop due to reduced herbicide breakdown. The present study aimed at exploring the contribution of bio-fertilizer microbes (Azospirillum) to mitigate the inhibitory effect of herbicide residue in soil, and their inhibitory effect on weed seeds. The cr...

 Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**

ECONOMICS OF COW MILK PRODUCTION IN SINDH AND AZAD JAMMU & KASHMIR
...ed through simple random sampling technique. Shares of different feed resources in total feeding cost have been determined and cost-benefit analysis of milk production by ecological zones was also conducted. Wide variations in share of feed sources in total feeding cost per annum, milk productivity and profitability of main cow breeds across selected areas have been observed. Concentrate feeding is the main cost item for cow milk production in irrigated areas ...

Tanzila Kazmi*, Abid Ghafoor Chaudhry*, Aftab Ahmed** and Shaheer Ellahi Khan**

FARMERS BELIEFS ABOUT INDIGENOUS FARMING PRACTICES AND SUSTAINABLE AGRICULTURAL DEVELOPMENT
...l technique in which the sample of 187 farmers were drawn out of the population of 3680, to cultivate insight and beliefs regarding the indigenous farming practices and how it is related with sustainable agricultural development. During the study a mixture of qualitative techniques including interview guide(s), focus group discussions (FGDs) and case study were used. The study revealed that indigenous knowledge is a long term process and demands a complete app...

 Naveed Jehan*, Khalid Mahmood Aujla**, Muhammad Shahzad*, Abid Hussain**, Muhammad Zahoor*, Majid Khan* and Ahmed Bilal*

USE OF MOBILE PHONES BY FARMING COMMUNITY AND ITS IMPACT ON VEGETABLE PRODUCTIVITY
...armers from district Charsadda. The data was analyzed through simple averages, correlation analysis and paired t-test. Average yield of bitter -1 gourd crop was 23569 kg acre with cell phone use of 8h per season. It is perceived that low communication cost and availability of information about wholesale market prices through SMS service and low cost calling packages will help farmers and market agents in improving bitter gou...

 Ayesha Tahir*

FORECASTING CITRUS EXPORTS IN PAKISTAN
... 2314.46 and 2564.00 thousand tons and forecasted export values are 673.67, 782.34, 898.57, 1022.35 and 1153.68 thousand dollars. Forecasted values are very close to actual values and have positive increasing trend in Pakistan.

...

 Muhammad Hanif Munawwar*, Asghar Ali* and Shahid Riaz Malik*

IDENTIFICATION OF RESISTANCE IN MUNGBEAN AND MASHBEAN GERMPLASM AGAINST MUNGBEAN YELLOW MOSAIC VIRUS
...gainst mungbean yellow mosaic (begomo) virus (MYMV), 64 lines of mungbean and 21 of mashbean were evaluated under field conditions. Out of 64 mungbean lines, 6 were found as resistant (R) and 35 moderately resistant (MR). Sixteen accessions were graded as susceptible (S) whereas 7 were found highly susceptible (HS). In mashbean, 12 out of 21 accessions were resistant (R) and 8 moderately resistant (MR), whereas, only one was susceptible. Although resistance ag...

 Nazir Ahmad*, Sultan Ayaz*, Sumaira Shams**, Karimullah*** and Riaz Ahmad****

PREVALENCE AND MORPHOLOGY OF HELMINTH PARASITES OF FISH FROM RIVER SWAT, KHYBER PAKHTUNKHWA
...ephalus, Rhabdochona charsaddiensis, Rhabdochona schizothoracis and Neoechynorhynchus devdevi. They were identified by morphological characteristics through microscopic techniques. Overall prevalence of the fish parasites was 58% (145/250). Among these Schizothorax plageostomus fish 93.04% (107/115), Schizothorax labiatus 61.11% (33/54), Salmo trutta fario 17.85% (05/28), Gara gotyla 0% (0/09), Rita rita 0% (0/25) and Oncorhynchus mykiss were 0% ( 0/19). The i...

 Rehmat Ullah*, Kalim Ullah** and Mohammad Zafarullah Khan*

EXTENSION SERVICES AND TECHNOLOGY ADOPTION OF DATE PALM (PHOENIX DACTYLIFERA L.) IN DISTRICT DERA ISMAIL KHAN
... interview method from a sample of 51 respondents selected from these villages. Agriculture was the major profession of the farmers (80.3%). It was found that 62.7% of the respondents were never visited by the extension workers while 60.7% of the respondents never attended any meeting with field staff or Agriculture Officers. A single field day was organized by extension workers almost two years ago in the studied area attended by only 41% of the respondents. ...

 Muhammad Sharif*, Muhammad Azam Niazi*, Abdul Jabbar** and Sajida Taj*** 

FINANCIAL PROFITABILITY OF WHITE SUGAR PRODUCTION IN PAKISTAN
... respectively during the same -1 -1 period. The per capita availability (consumption kg person year ) was 27.18 in Pakistan. Urgent research efforts are required to ensure development of improved varieties of sugarcane with higher sucrose recovery. Effective measures are needed for disease control and optimal pesticide use. Adequate supply of water, optimal use of fertilizer and judicious use of pesticides can improve yield and hence higher financial profitabi...

 Abid Hussain*, Khalid Mahmood Aujla* and Nouman Badar*

YIELD GAP DETERMINANTS FOR WHEAT PRODUCTION IN MAJOR IRRIGATED CROPPING ZONES OF PUNJAB, PAKISTAN
... number of irrigations, usage of farm yard manure and fertilizers contribute positively and significantly to wheat crop production. Coefficients of dummy variables for cropping zones indicate that farmers in the mixed cropping zone are obtaining better yield of the wheat crop as compared to their counterparts in other selected cropping zones. These results suggested that farmers can increase wheat productivity by increasing the use of factor inputs; however, p...

 Sajid Ali*, Quratulain Altaf** and Umar Farooq* 

ACREAGE RESPONSE OF FLUE CURED VIRGINIA TOBACCO IN KHYBER PAKHTUNKHWA
..., Swabi, Mardan, and Charsadda. Data used in the study cover time series data for 1971-2011. The newly developed Auto Regressive Distributed Lag (ARDL) model for cointegration was used to estimate the short-run and long-run elasticities. The study found a long-run price elasticity of 0.33, thereby revealing that FCV acreage response to its own price is relatively inelastic. The short-run acreage response was also low (0.13) and therefore relatively inelastic. ...

 Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****

GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY

 Samiullah*, Mehmood Shah*, Kalim Ullah**, Rehmat Ullah*** and Ibrarullah****

PROFITABILITY OF WHEAT PRODUCTION IN DERA ISMAIL KHAN
...roducing villages in the said district. A total of 50 respondents were probed by personal interview method from the sampled villages. Cost and return (gross return) data from the respondents were analyzed. Farm budgeting technique for estimating cost, gross return and profitability was applied. The analysis of data revealed that the average cost per acre was Rs. 24716.32. Similarly average production of wheat (output) was es...

 Aniqa Iram*, Javed Khan**, Nadeem Aslam**, Ehsan-ul-Haq**, Habib Iqbal Javed**, Muhammad Irfan*, Awais Rasool*, Muhammad Ishaque Mastoi** and Sumera Aslam* 

EFFICACY OF PLANT DERIVED OILS AND EXTRACTS AGAINST WHITEFLY, BEMISIA TABACI (GENNADIUS) ON SESAME CROP
... and taramera oil (Eruca sativa) against whitefly, Bemisia tabaci on sesame crop. The data were recorded 24h before and 24h, 48h, 72h and 168h after application of each spray material. The results showed that whitefly population was significantly suppressed by both the botanical oils and extracts as compared to the control treatment but in general botanical oils showed significant results as compared to plant extracts. Soybe...

 Naheed Zahra*, Hassnain Shah*, M. Azeem Khan* and M. Zubair Anwar* 

LIVELIHOOD ASSETS AND POVERTY NEXUS: A CASE STUDY FROM RAINFED POTHWAR AREA OF PAKISTAN
...ry data collected from a sample of 40 households arround watershed project site at Fateh Jang in Pothwar region. The inter-linkage of livelihood assets and poverty is determined by employing poverty score card technique. The poverty status of household was highly correlated with the livelihood sources (P<0.01), water availability for irrigation(P<0.10) and tenancy status (P<0.05). The results regarding poverty revealed that 2.50% of the

 Munir Ahmad*, Asif Ali Mirani**, Hafiz Sultan Mahmood**, Tanveer Ahmad** and Muhammad Azhar Saeed**

PERFORMANCE EVALUATION OF CROSS-FLOW RICE DRYERS
... the gas-fired dryer was satisfactory, while that of the rice husk-fired dryer can be improved by incorporating an appropriate heat exchanger.

...

 Gul Zamin Khan*, Imtiaz Ali Khan**, Inamullah Khan* and Mian Inayatullah**

OUTDOOR BREEDING OF MOSQUITO SPECIES AND ITS POTENTIAL EPIDEMIOLOGICAL IMPLICATIONS IN KHYBER PAKHTUNKHWA
...Nowshera, Mardan and Charsadda of Khyber Pakhtunkhwa, Pakistan during 2012-13 for development of ultimate control strategies of mosquito breeding in outdoor habitats. Larvae were collected from various mosquito breeding habitats such as irrigation channels, irrigation water leakages, pots, vase, tyres, temporary containers stagnant flood waters, etc. Ovitraps were used as monitoring tools in urban areas of the selected sites. Results showed that mosquitoes are...

 Hassnain Shah*, Nadeem Akmal*, Waqas Farooq*, Muhammad Azeem Khan* and Shahid Munir**

SHIFT FROM SITE DEVELOPMENT TO SKILL DEVELOPMENT: A CASE STUDY OF WATER HARVESTING THROUGH MICRO CATCHMENTS
...ater, labor and resource saving along with farmer friendly under rainfed condition with scare supplemental water availability. The cost of adoption of technology including labor charges for its preparation and maintenance was recovered from irrigation cost saved from single rainfall at the demonstration site. One of the important implications drawn in this regard was the need of a shift from site development to skill develop...

 Ambreen Akhtar Saddozai*, Amer Mumtaz*, Nouman Rashid*, Shahzada Arshad Saleem**, Saeeda Raza* and Muhammad Naeem Safdar*

COMPARISON OF INDIGENOUSLY DEVELOPED TANGERINE-ORANGE MARMALADE WITH BRANDED MARMALADES AVAILABLE AT LOCAL MARKETS
...butes. TPC count in both samples were nil. Yeast and -1 mould count was nil in branded while it was 600 and 400 cfug in orange and tangerine marmalades, respectively. Sugar contents, pH and Brix ranged from 14.97% to 59.70%, 3.00 to 3.46, 15.0 B to 60.2 B in branded and non-branded marmalades, respectively. Organoleptically marmalade developed at FSPDI was non-significantly different from all the branded marmalades except Salman’s.

...

 Muhammad Sharif*, Muhammad Azam Niazi* and Abdul Jabbar**

SEAFOOD EXPORTS: CHALLENGES AND WAY FORWARD
...w but dwindling to the disastrous level. Export of seafood has increased from 131,620 t (a value of US$ 315.5 million) in 2011-12 to 136,360 t (a value of US$ 333.13 million) in 2012-13, with an annual increase of 9.5% in term of quantity and 4.5% in term of value. Decline in seafood resources, lack of shrimp culture, insufficient facilities at fish markets, inadequate fishing policies and low export prices of seafood are major challenges faced by fisheries se...

 Nosheen Zahra*, Ghulam Sarwar* and Sher Muhammad**

COMPARISON OF GYPSUM AND POTASSIUM SILICATE FOR RECLAMATION OF SALINE SODIC SOIL
...evels for reclamation of saline sodic soil was tested. Treatments were replicated thrice. All pots were arranged according to completely randomized design and treatments were applied to the soil according to treatment plan. Appropriate time was given to achieve the reclamation of saline sodic soil and relative efficiency was assessed through laboratory analysis. After this, rice seedling (Shaheen Basmati) was transplanted in...

 Usman Shakoor*, Abdul Saboor*, Irfan Baig*, Anila Afzal** and Abdul Rahman***

CLIMATE VARIABILITY IMPACTS ON RICE CROP PRODUCTION IN PAKISTAN
...dequate policy action to safeguard crop productions from disastrous effects. Development of varieties resistant to high temperatures as well as droughts will definitely enhance resilience of rice crop in Pakistan. 

...

 Muhammad Aamir Khan*, Abdul Saboor*, Rauf-i-Azam**, Abdul Qayyum Mohsin* and Fayyaz-ul-Hassan***

IMPACT OF AGRICULTURAL TRADE LIBERALIZATION ON INCOME INEQUALITY IN PAKISTAN
... and labor types. This disaggregation allows to analyze distributional impacts and to implement policy scenarios that target particular households. The economy wise results showed that agricultural trade liberalization increases income inequality and somewhat detrimental to the rural household types, who guage most of their income from agricultural business. Trade policy must be pursued keeping derivation and distributional narration in view. 

...

 Maimoona Bashir*, Imtiaz Ahmad Qamar*, Muhammad Fateh Ullah Khan* and Abdul Razzaq*

EFFECT OF MIXING LOW PALATABLE GRASSES AND IPIL IPIL LEAVES ON FORAGE QUALITY

 Muhammad Shahzad*, Ayesha Tahir**, Naveed Jehan*** and Muhammad Luqman*

IMPACT OF DIFFERENT PACKAGING TECHNOLOGIES ON POSTHARVEST LOSSES OF STONE FRUITS IN SWAT PAKISTAN
... by proportionate random sampling procedure. Quantitative losses were estimated through percentage method while partial losses were estimated at the wholesale and retail level by price differential method. Multiple regression analysis was employed to find relation between post-harvest losses and different factors at three different stages. Findings of the study revealed the channel of cardboard box technology accounted for p...

 Muhammad Ayub*, Rizwan Maqbool*, Muhammad Tahir*, Zoaib Aslam*, Muhammad Ather Nadeem* and Muhammad Ibrahim**

IMPROVED GROWTH, SEED YIELD AND QUALITY OF FENNEL (FOENICULUM VULGARE MILL.) THROUGH SOIL APPLIED NITROGEN AND PHOSPHORUS
...ce oil content, under Faisalabad conditions.

...

 Mazher Abbas*, Irfan Mehmood*, Arshed Bashir*, Muhammad Ather Mehmood* and Sonila Hassan* 

WOMEN COTTON PICKERS PERCEPTIONS ABOUT HEALTH HAZARDS DUE TO PESTICIDE USE IN IRRIGATED PUNJAB
...n health costs) of using safety measures during and after cotton picking.

...

 Arshed Bashir*, Fatima Ahmad**, Irfan Mehmood*, Muhammad Qasim*, Mazher Abbas* and Sonila Hassan*

ECONOMICS OF RED MEAT PRODUCTION IN PUNJAB
... zone and Chiniot and Faisalabad from mixed cropping zone) were selected for data collection and 76 red meat producers were interviewed. The results showed that commercial farms were dominated by cows while the small sized specialized farms were dominated by buffaloes. The mean age, sale price and live weight of sold buffaloes was 2.8 years, Rs. 64963.8 and 157 kg, respectively whereas for cows the mean age was 3 years with ...

 Muhammad Akhter*, Muhammad Azhar Ali**, Zulqarnain Haider* and Shahzad Muzammil***

PHYSICO-CHEMICAL CHANGES IN GRAINS OF SOME ADVANCE LINES/ VARIETIES OF RICE (ORYZA SATIVA L.) AFTER PARBOILING
...illed and parboiled rice samples of ten lines/varieties were analyzed for nutritional quality parameters such as ash, dry matter, crude fat, crude protein, crude fiber, vitamin B6; milling quality parameters such as total milling recovery, head rice recovery, ratio of broken grains and cooking quality parameters such as curling, bursting and cooked grain length. The study showed significant variation in efficacy of parboiling to different varieties/lines. The ...

 Muhammad Arshad Ullah*, Nazir Hussain**, Helge Schmeisky*** and Muhammad Rasheed**** 

INOCULATION AND INTER-CROPPING OF LEGUMES IN ESTABLISHED GRASS FOR INCREASING BIOMASS OF FODDER
...grass and legumes (Vicia sativa and cowpeas) coupled with inoculation was studied under rainfed conditions at National Agricultural Research Centre (NARC) Islamabad, Pakistan. Intercropping significantly increased tillering of grass. Seed inoculation of legumes also gave maximum tillers. The grass and legumes biomass without any treatment were recorded as 7.09 and -1 8.17 t ha , respectively, during two years of study. Mixed fodder -1 production increased to 1...

 Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***

CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...ut of 44 from plant root samples along with rhizospheric soil, collected from different salt affected areas of Central Punjab (Pakistan). Selected bacterial strains were characterized morphologically as well as biochemically at National Agricultural Research Centre, Islamabad during 2011-13. It was observed that all isolates produced IAA, whereas14 strains were declared as phosphate solubilizing bacteria (PSB), eight isolate...

 Anum Fatima*, Saleem Abid* and Sobia Naheed* 

TRENDS IN WHOLESALE PRICES OF ONION AND POTATO IN MAJOR MARKETS OF PAKISTAN: A TIME SERIES ANALYSIS
...amine the trends in wholesale prices of onion and potato in the major markets (Lahore, Hyderabad, Peshawar and Quetta) of Pakistan. Time series data about annual average wholesale prices of onion and potato were collected from 1981-82 to 2011-2012. Major findings of the study revealed that wholesale prices of onion and potato were increased with time. Results revealed that whole

 Rashed Saeed*, Shoaib-ur-Rehman**, Muhammad Qasim*, Hafiz Zahid Mahmood*** and Irfan Mehmood*

ECONOMICS OF PERI-URBAN RADISH PRODUCTION AND MARKETING IN FAISALABAD
...rban area of district Faisalabad, Pakistan. It was found that majority of farmers were not following recommendations of the agriculture department regarding seed rate, fertilizers, and irrigations. Majority of respondents (94.2%) reported the local Meno as the best yielding variety. Imported and 40 day varieties were not popular as only 5.8% farmers were cultivating these varieties in the study area. On the basis of survey data, benefit cost and total factor p...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...were then homogenized in saline and centrifuged prior to biochemical analyses. Results showed differences in the activities of enzymes and quantities of total protein, soluble protein and FAA between strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20 /levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses i...

 Khalid Mahmood Aujla* and Abid Hussain*

ECONOMICS OF MILK PRODUCTION OF MAJOR DAIRY BUFFALO BREEDS BY AGRO-ECOLOGICAL ZONES IN PAKISTAN

Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***

ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...ontrol measures are necessary to prevent ND outbreaks. Natural resources have been exploited to obtain antiviral compounds in several latest studies. In this study, the antiviral activity of Viro Care GZ-08™ was checked up invitro, in-ovo and in-vivo. The cytotoxicity assay of the product was performed using Vero cell line. All the trials revealed that the stock solution and 1:2 dilution of GZ-08™ had some antivi...

 Muhammad Asif Jamil*, Nowshad Khan*, FarhatUllah Khan* and Muhammad Zakria*

EFFECTIVENESS OF AGRICULTURAL EXTENSION SERVICES IN DISSEMINATION OF INFORMATION TO THE FARMING COMMUNITY OF DISTRICT BHIMBER, AZAD JAMMU AND KASHMIR
... Kashmir. The convenient sampling as well as multistage sampling method was used to collect the data from the study area. The overall data consists of 150 farmers. The study revealed that respondent's acquaintance with extension staff was very low as only 18.00% of the respondents had personal acquaintance with the extension staff and 12.67% of them knew extension staff through their neighbour, while 38% of respondents from ...

 Syed Asif Imran Shah*, Shah Jehan Khan**, Abdul Aziz Baloch*** ,
Obaid Ullah Sayal**, Kalim Ullah* andShujaat Ali**

COMBINING ABILITY ANALYSIS IN VARIOUS INBRED LINES OF MAIZE (ZEA MAYS L.)

 Sameera Arshad*, Iftikhar Hussain*, Maqsood Anwar* and Sarwat Naz Mirza*

HABITAT SURVEY FOR RECOGNIZING BIRD ATTRACTANTS AROUND BENAZIR BHUTTO INTERNATIONAL AIRPORT, ISLAMABAD, PAKISTAN
...h in return is a serious safety concern for aircraft operation. Outcome of this study could be incorporated into off airfield bird management programme for monitoring bird activity and land use practices around the airfield.

...

 Junaid Alvi*, Ijaz Ashraf*, Khalid Mehmood Ch*, Muhammad Iftikhar* and Saleem Ashraf**

IMPACT OF LIVESTOCK IN UPLIFTING RURAL LIVELIHOOD
...Jaranwala of District Faisalabad. Data showed that, livestock farming on small level was found widely adopted for income generation. More than 22% respondents earned a maximum income of more than Rs.15000. Livestock have dominant effect on domestic needs fulfillment. Farmers were spending income on family chores, education, health and other aspects of life. Informal discussions and observation dictated the lower productivity than the potential and inadequate a...

 Nouman Badar*, Hina Fatima**, Abdul Jabbar*** and Muhammad Asif*

MAJOR FOOD CROPS PRODUCTION AND YIELD FORECAST IN PAKISTAN
...to be -1 about 37188 thousand tons and 3454 kg ha , respectively. The rice (production -1 and yield) forecast for 2029-30 will be about 7176 thousand tons and 2630 kg ha , respectively. The maize (production and yield) forecast for 2029-30 will be about -1 5784 thousand tons and 6088 kg ha , respectively. In short, the production growth rate of wheat, rice and maize was higher in 2012.

...

 Khurram Nawaz Saddozai*, Umme Rubab* and Abass Ullah Jan* 

STOCHASTIC FRONTIER ANALYSIS OF MAIZE FARMERS IN AZAD JAMMU AND KASHMIR, PAKISTAN
...kistan. The proportional sampling allocation sampling technique was adopted to collect primary data from 80 sampled respondents in 2013-14. The maximum likelihood estimates of major inputs showed that seed, tractor hours, FYM and labor days have contributed significantly to increase the maize yield. However, the DAP and urea have shown no effect on maize yield. The mean technical efficienc...

 Khurram Nawaz Saddozai*, Muhammad Nasrullah* and Noor P. Khan** 

STOCHASTIC FRONTIER PRODUCTION ANALYSIS OF TOBACCO GROWERS IN DISTRICT MARDAN, PAKISTAN
...aned during 2014-15 from sampled population of three villages namely Takkar Kali, Garo Shah and Passand Kali of Takhtbhai Tehsil, Mardan district of Khyber Pakhtunkhwa province. The multi-stage sampling technique was utilized to obtain the desired sample size of 120 tobacco growers. The major findings of stochastic production frontier analysis indicate t...

 Umar Ijaz Ahmed*, Liu Ying**, Muhammad Khalid Bashir***,
Muhammad Amjed Iqbal*, Muhammad Rizwan*, Muhammad Mazhar
Iqbal****, Muhammad Rafi Qamar***** and Aftab Nazeer******

SPATIAL PRICE TRANSMISSION IN PAKISTAN: THE CASE OF WHEAT AND RICE MARKETS
...nal and bidirectional causality exists between wheat and rice markets. Bad and poor infrastructure is a major impediment to price transmission among the markets. These market imperfections lead to food insecurity in the country. Government should formulate better policies and develop infrastructure towards better and efficient market function. The results of this study will help the policy makers to formulate a better policy to enhance marketing function to ov...

 Zafar Abbas*, Ijaz Ahmad**, Adnan Shakeel**, Muhammad Abdullah***, Muhammad Islam**, Sadiq Muhammad*, Ghulam Murtaza* and Mushtaq Ahmad**

EFFECT OF PHOSPHORUS FERTILIZER AND WATER STRESS ON PROTEIN AND PHENOLIC CONTENTS IN COTTON (GOSSYPIUM HIRSUTUM L.)

 Raja Zoq-ul-Arfeen*, Aamir Saleem*, Sarwat Naz Mirza*, Muhammad Akmal**, Hafiz Muhammad Tayyab* and Obaid Afzal***

BIODIVERSITY AND PHYTOSOCIOLOGICAL STUDIES OF UPSTREAM AND DOWNSTREAM RIPARIAN AREAS OF PAKISTAN: SPECIAL REFERENCE TO TAUNSA WILDLIFE SANCTUARY AND KETI SHAH FORESTS
...Keti Shah forest and Taunsa wildlife sanctuary. Total 14259 plants/individuals were recorded, which belong to 54 plant species with 18 different families. In Taunsa pre-monsoon survey, total 30 plant species were found with 4476 plants from 16 different families. In Taunsa post-monsoon survey total 3348 individuals were recorded from 20 plant species and...

 Ambreen Akhtar Saddozai*, Amer Mumtaz*, Saeeda Raza* and Shahzada Arshad Saleem** 

MICROBIAL COUNT AND SHELF LIFE OF PHALSA (GREWIA ASIATICA) JUICE
...laboratory developed phalsa juice at room temperature. Phalsa was purchased from local market, juice was prepared and kept in sterilized bottles at room temperature. Physicochemical and microbial and oragnoleptic quality of the juice was examined till two weeks. Microbial activity in phalsa juice increased while organoleptic attributes such as texture (mouth feel), flavour, taste, colour a...

 Anwar Hussain* and Muhammad Rahman**

ROLE OF TRAININGS ON FARMERS' PROFITABILITY OF MEDICINAL AND AROMATIC PLANTS IN MOUNTAINOUS AREAS OF DISTRICT SWAT, KHYBER PUKHTUNKHWA
...trict Swat. A convenient sample of 100 respondents, engaged in MAPs in Chail Valley Madyan was taken. Information about revenue from MAPs, marketing, prices and quantities was collected through a structured questionnaire. The respondents were given training under Swiss Development Cooperation Project at the time of collection, harvesting, cleaning, drying, packing and marketing of MAPs. The overall findings revealed that the training had positive impact on far...

 Muhammad Nisar Khan*, Tariq Hassan*, Hassnain Shah*, Saleem Abid*, Irum Raza* and Saqib Shakeel Abbasi*

ASSESSMENT OF THE PROFESSIONAL TRAINING COURSE UNDER WATERSHED PROJECT AT FATEHJANG FIELD STATION, PUNJAB, PAKISTAN
...te awareness about water saving technologies as solar powered irrigation system, drip irrigation, sprinkler irrigation, micro catchment, rooftop rainwater harvesting and irrigation scheduling. Therefore, the project activities also include the training of the professionals from the line departments for the demonstrated technologies to foster the process of adoption. For this purpose Climate and Alternate Energy Water Resources Institute, NARC, organized a prof...

 Imdad Ali Mahmood*, Arshad Ali**, Armghan Shahzad**, Muhammad Asif Masud Ghumman***, Muhammad Arshad Ullah**, Tariq Sultan** and Badar-u-Zaman**

CROP RESIDUES AND PHOSPHORUS EFFECT ON YIELD AND ECONOMICS OF DIRECT SEEDED RICE AND WHEAT GROWN UNDER SALINE-SODIC SOIL
...p; A field study on saline-sodic sandy loam soil (EC =6.6 dS m ; pH e -1 1/2 -1 =8.31; SAR =17.4 (m.mol L ) ; CaCO =3.5% and available P=3.9 mg kg ) was c 3 conducted on direct seeded rice and wheat for two consecutive years at Soil Salinity Research Institute (SSRI) Farm, Pindi Bhattian, Pakistan to investigate the yield and economics of direct see...

 Azhar Usman Ali*, Ghulam Sarwar*, Muhammad Aftab* and Sher Muhammad**

EFFECT OF SOIL AND FOLIAR APPLIED COPPER ON GROWTH AND YIELD OF WHEAT (TRITICUM AESTIVUM L.)
...For -1 this purpose, non-saline non-sodic soil (pH = 8.10, EC = 0.85 dS m , SAR = 4.60, saturation percentage = 29 % and sandy clay loam) was collected, brought to -1 green house and filled in the pots @ 6.5 kg pot . All these pots were irrigated to achieve uniform soil column. This experiment comprised 5 treatments vi...

  Sohaib Arshad*, Sarwat Naz Mirza*, Imtiaz Ahmad Qamar** and Maqbool Shahbaz**

DETERMINATION OF FORAGE QUALITY OF INDIGENOUS AND EXOTIC RHODES GRASS ACCESSIONS UNDER RAINFED CONDITIONS

 Muhammad Qasim*, Khuda Bakhsh**, Sultan Ali Tariq*, Mahmood Nasir***, Rashed Saeed**** and Muhammad Ather Mahmood****

FACTORS AFFECTING GROUNDNUT YIELD IN POTHWAR REGION OF PUNJAB, PAKISTAN
...fitability analysis. The sample farms were classified into small, medium and large farms. Farm-level crop data were gathered during two cropping seasons i.e., rabi 2008-09 and kharif 2009. One hundred and forty groundnut producers were selected for collecting data using the well-structured questionnaire from two important districts recognised for area and production of groundnut. Results showed that large farmers allocated significantly higher area (34%) to gr...

 Khalid Mahmood Aujla* and Abid Hussain*

MARKETING SYSTEM OF LIVE CAMELS IN THE DESERT ECOLOGIES OF PAKISTAN
... and buyers can make transactions. This study was designed to investigate the existing marketing system of live camels in the desert ecologies of Pakistan. Out of 220 camel farmers that were randomly interviewed for the study, during past one year sale of live camels was reported by more than half of the camel farmers. On an average two camels per farm mainly to meet urgent family cash needs. Mean ages of adult males, milkin...

 S. Talat Gilani*, Shahid Hameed* and Hussain Shah* 

OCCURANCE AND DISTRIBUTION OF POTY VIRUSES INFECTING GARLIC IN PAKISTAN
...seases in garlic (Allium sativum) from major garlic growing areas of Pakistan. The yellow stripes, mosaic and chlorotic spot symptoms of the disease resemble the viral infection in garlic reported to occur worldwide. Altogether 690 samples were collected from 29 locations of Punjab and 40 locations of Khyber Pukhtunkhwa to determine the prevalence of Onion Yellow Dwarf Virus (OYDV) and Lee...

Sumia Bint Zaman*, Waqas Farooq*, Sidra Majeed*, Hasnain Shah*, and Abdul Majid**

ASSESSMENT OF AGRICULTURE SERVICE PROVIDERS' TRAINING ON WATER CONSERVATION TECHNOLOGIES IN POTHWAR REGION
...Results indicated higher satisfactory level as majority of the participants were strongly agreed (73%) and agreed (23%) that trainings were participatory and skill development oriented and as 96% agreed or strongly agreed that these were helpful in starting the business as assessment of agriculture service providers (ASPs). It can be concluded that the necessary condition has been fulfilled for water conservation. However, f...

 Muhammad Farrukh Saleem*, Munir Ahmad*, Shakeel Ahmad Anjum*, Muhammad Aown Sammar Raza** and Abdul Shakoor*

EFFECT OF TOPPING UNDER DIFFERENT NITROGEN LEVELS ON EARLINESS AND YIELD OF COTTON
...rsity of Agriculture, Faisalabad, during kharif -1 2013. The crop was sown on ridges on May 24, 2013 using seed rate of 20 kg ha . The experiment was laid out in Randomized Complete Block Design (RCBD) with factorial arrangement having three replications. Treatments included four topping levels: control (no topping), topping at 90 cm, 120 cm and 150 cm height; three -1 nitrogen levels: 150, 200 and 250 kg ha . Net plot size was 6 m × 3 m. Maximum days to...

 Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*

HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...A series over-expressing same gene (pCOsRGLP1). A lower number of explants generated hygromycin resistant plantlet for instance, 18.3 with pGOsRGLP1 vector as compared to 35.5% with pCOsRGLP1 vector. Transformation efficiency of PCR positive plants generated was 9.4% for pGOsRGLP1 while 21.6% for pCOsRGLP1. Furthermore 24.4% of explants -1 generated antibiotic resistant plantlet on 20 mgl of hygromycin which was -1 higher than on 15mgl of hygromycin such as 12...

 Muhammad Tahir* and Nawal Zafar* 

FORAGE YIELD AND QUALITY PERFORMANCE OF RABI CEREALS SOWN ALONE AND IN BLENDED POPULATION AT VARIABLE SEED RATIOS
...rsity of Agriculture, Faisalabad, during 2013-14. The results showed that the crop mixtures and their variable seed ratios showed significant effects on fodder yield and quality traits. -1 The maximum number of tillers, number of leaves plant , leaf area, crop growth -1 -1 rate, fresh weight plant , dry weight plant , green forage yield and dry matter yield were obtained in plots where barley was sown alone at 100% seed ratio. The highest crude fiber and total...

 Muhammad Mansoor*, Noman Latif*, Zafar Islam**, Sher Muhammad**, Amanullah* and Muhammad Umair***

IMPACT ASSESSMENT OF SOIL AMENDMENTS ON SOIL CAPPING IN MUNGBEAN PRODUCTION USING CENTRAL PIVOT IRRIGATION SYSTEM IN CLAYEY SOILS
...carried out through pure sand and farm yard manure (FYM) in equal proportions and in three different application rates viz., 60:60, -1 45:45 and 30:30 kg plot and an un-amended soil plot was kept as control. The size of each plot was 4m x 3m. The results depicted that parameters such as number of branches per plant, number of pods per plant, pod length, number of grains per pod, 1000-grain weight, and grain yield were significantly affected by different soil a...

Muhammad Zahid Kiani*, Tariq Sultan**, Arshad Ali**, Ghulam Qadir*** Imdad Ali Mahmood**, Tauseef Tabassam**, Muhammad Arshad Ullah** and Nasir Abbas* 

EFFECT OF PGPR STRAINS ON SUNFLOWER GROWTH AND NUTRIENT CONTENTS UNDER SALINITY STRESS
...rtificially developed -1 salinity levels (5, 8, 10 and 12 dS m ) at National Agricultural Research Centre, Islamabad. Hybrid seeds (SMH 0917) of sunflower (Helianthus annuus L.) were used. After six weeks of sowing, plants were harvested and data regarding root length, plant height, root dry weight and shoot dry weight were recorded. The plant growth improved significantly with PGPR strain under salt stress. The root length,...

Samina Qamer*, Iram Nasir**, Um-Habiba Zafar**, Salma Sultana** and Tayyaba Sultana**

BIO-CHEMICAL EVALUATION OF HONEY PRODUCED BY APIS MELLIFERA HONEYBEE COLLECTED FROM JHANG, BAHAWALPUR, MULTAN, JHELUM AND KALAR KAHAR AREAS
...l (HMF) content of honey samples were measured as 30.25 mgkg -1 from Jhang, 18.10 mgkg -1 from Kalar Kahar, 25.29 mgkg from Bahawalpur,19.71mgkg fromMultan, and19.62mgkg fromJhelum, respectively.

...

 Farid Asif Shaheen*, Sadia Parveen*, Ahmed Zia**, Ghulam Qadir*,
Mureed Husain*** and Rifat Ullah Khan****

PREDATORY APTNESS OF ANTS AGAINST RED FLOUR BEETLE, TRIBOLIUM CASTANEUM HERBST (TENEBRIONIDAE: COLEOPTERA) IN WHEAT FLOUR
...onsumption. The infested samples of wheat flour by T. castaneum were collected from different localities and its culture was maintained in laboratory. Three ant species namely, Dorylus labiatus, Camponotus rufipes and Monomorium minimum were collected from forest and non-forest habitats and compared for their predation against different life stages of RFB. Results showed D. labiatus of forest habitat as an efficient pupal predator that consumed 91.66% pupae of...

 Waqar Akhtar*, Muhammad Sharif**, Abdul Hayee Qureshi*, Khalid Mahmood Aujla*** and Muhammad Azeem Khan*

COMPETITIVENESS OF TOMATO PRODUCTION IN PUNJAB, PAKISTAN
...urtail tomato imports to save the precious foreign exchange. To enhance the competitiveness there is need to increase farmer's incentives through increase of farm level price up to import parity prices of tomato through efficient marketing. Technological improvement in production, marketing and value addition of tomato is also needed to address farm and market level issues of competitiveness.

...

 Ghulam Sadiq Afridi*, Abdul Saboor**, Zahoor-ul-Haq***, Sultan Ali
Tariq**** and Muhammad Ishaq*****

EXPLORING POTENTIAL AND OPPORTUNITIES FOR PAKISTAN'S COTTON EXPORT

 Shahid Iqbal Khattak*, Muhammad Amjad Nadim**, Mohammad Safdar Baloch**, Kashif Waseem** and Muhammad Sohail***

IMPACT OF VARIABLE SEEDING DATES ON CEREALS' PERFORMANCE UNDER AGRO-ECOLOGICAL CONDITIONS OF DERA ISMAIL KHAN
...9.08 days) which led the same towards earlier maturity (131.21 days) while oat crop showed prolonged maturity (147.67 days). Regarding yield, the four cereals showed non-significant results. Among different dates, January 1 sowing had the poorest performance in almost all parameters. However, the highest grain yield (3104 kg ha ) was produced by October 15 sowing while the results of November 15 and thereafter sowing were nonsignificant with each other. Data o...

 Inayat-Ur-Rahman*, Shamsher Ali**, Mukhtar Alam*, Mohammad Adnan*, Hidayat ullah*, Muhammad Faisal Anwar Malik*, Abdul Sattar Shah* Mohammad Ibrahim*

EFFECT OF SEED PRIMING ON GERMINATION PERFORMANCE AND YIELD OF OKRA ( L.)

 Dilawar Khan*, Abid Saeed*, Junaid Ahmad*, Imtiaz Qamar**, Fazal Yazdan**, Saleem ud Din** and Muhammad Tariq**

ASSESSMENT OF RIPARIAN VEGETATION IN DHRABI WATERSHED AND CHAKWAL REGION IN PAKISTAN
...) by using line transect sampling method. On the basis of top three highest IV plant community were identified at western side of the Kallar Kahar Lake while plant community was observed in eastern side by using 1x1 m quadrat method.

...

 Hafiz Sultan Mahmood*, Tanveer Ahmad*, Zulfiqar Ali*, Munir Ahmad** and Nadeem Amjad***

FIELD EVALUATION OF A WHEAT STRAW CHOPPER
...arvested wheat straw and saves the environment from smoke pollution, if straw is burnt in the field. Field performance of this technology has not been evaluated yet, although this technology has been adopted in Pakistan for many years. The objective of this study was to evaluate the performance of an improved version of wheat straw chopper in combine-harvested wheat fields of southern Punjab and to determine the economics of this machine. Five test plots rangi...

Basharat Hussain Shah*, F. S. Hamid*, Shams ul Islam*, Fayaz Ahmad*, Sohail Aslam*, and Noorullah Khan*

EVALUATION OF DIFFERENT PEA (PISUM SATIVUM L.) GENOTYPES FOR YIELD AND OTHER ATTRIBUTES AT SHINKIARI, MANSEHRA
...arch Institute (AARI) Faisalabad and one local variety was included in the experiment as a control. The experiment was conducted during 2014-15 at National Tea & High Value Crops Research Institute (NTHRI) Shinkiari, Mansehra. Altogether seven pea genotypes were laid out in Randomized Complete Block Design (RCBD) with three replications. Normal agronomic practices were carried out during the growth period and data was recorded on different growth parameter...

 Ummad-ud-Din Umar*, Muhammad Aslam Khan**, *Ateeq-urRehman*, Abdul Hannan***, Syed Atif Hasan Naqvi*, Azhar Ali Khan****, Muhammad Asif Zulfiqar****

DEVELOPMENT AND VALIDATION OF POTATO LEAF ROLL VIRUS DISEASE PREDICTION MODEL BASED ON ENVIRONMENTAL FACTORS FOR FAISALABAD, PAKISTAN
...ars data collected in Faisalabad, Pakistan. Maximum and minimum air temperatures, relative humidity and rainfall were employed as independent variables while PLRV disease incidence served as the response variable. Stepwise regression analysis was performed to select potentially useful predictor variables for model development. In stepwise regression analysis minimum temperature, relative humidity and rainfall emerged as the main contributing environmental vari...

  Mohammad Umar Farooq* , Sarfraz Ahmad ** , Mohammad Islam and Imtiaz Ahmad Qamar ***

ASSESSING THE STATUS OF CARBON POOL IN TWO RANGELAND GRASSES ( ) IN POTHWAR, REGION, PAKISTAN
...mental plot. Twenty four samples of each experimental plot were collected with the help of ADC one m quadrat method, weighed and then oven dried at 60 C to find out the dry weight for above phytomass carbon Mg C ha . Similarly, the one m quadrats were dig out up to the root level for each grass and separated the root portion and weighed, oven dried at 60 C for below ground phytomass carbon Mg C ha estimation. Carbon pool in above ground phytomass was 1.16 Mg C...

 Muhammad Bilal*, Zafar Abbas*, Ijaz Ahmad**, Muhammad Aslam*, Muhammad Ijaz****, Adnan Shakeel***, Mushtaq Ahmad*** and Kashif Bashir*****

RESPONSE OF DIFFERENT COTTON ( L.) GENOTYPES AT VARIOUS SALINITY LEVELS
...nbsp;Keeping in view the salt stress threat in Pakistan, a hypothesis was developed that up to what level of salt, the cotton genotypes can survive so that it may provide the shelter for income from textile industry, as mainly the textile industry depends upon cotton production. High salinity levels hamper the boll formation in cotton genotypes as it reduces the yield. To verify this fact,...

 Muhammad Tahir* and Neelam Yasin*

EFFECT OF MICRONUTRIENTS FOLIAR APPLICATION ON YIELD AND QUALITY OF MAIZE
...rsity of Agriculture, Faisalabad . The treatments comprised of no micronutrient application (control), water spray and foliar application of micronutrients mixture @ 250, 500, 750, 1000, 1250 and 1500 ml ha at stem elongation stage of maize. All micronutrients treatments significantly improved plant height, cob length, number of grains rows cob , cob weight, 1000-grain weight, grain yield, biological yield, harvest index, grain protein and grain oil contents. ...

 Arshia Naeem*, Maria Anjum*, Mariam Rehman*, Zahid Mahmood** and Muhammad Asif Kamran**

AN INTEGRATED INFORMATION SYSTEM TO FACILITATE FARMERS IN WHEAT, SUGARCANE AND OTHER CROP DISEASES IDENTIFICATION
...dy was developed from Faisalabad region where information about crops, their soil conditions and associated diseases were provided on a web application. An online registration facility was provided along with interface to send image of crop related diseases. The desktop application consisted of an image analyzer that received images from web application and processes them to identify crop disease. The proposed platform was evaluated in collaboration with resea...

 Raza Ullah*, Mariam Rehman*, Maria Anjum*, Muhammad Asif
Kamran**, Khuda Bakhsh***, Abdul Saboor****

NAVIGATING FARMING RISKS BY SIMULTANEOUS DIVERSIFICATION AND CREDIT THROUGH FORMAL AND INFORMAL COMMUNICATION CHANNEL
... management tools at the same time. The decisions to adopt diversification and agricultural credit for risk management are correlated and adopting one risk management tool may make it more likely to adopt the other tool at the same time. Moreover, access to formal sources of information significantly encourages the use of both of the risk management tools to manage farm risk. Based on the information from the bivariate and m...

 Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********

SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...nducted to collect blood samples from small and large ruminants of subtropical rural communities in the province of Punjab during January-March, 2015. Total 59665 sera samples from apparently healthy animals were collected and screened by Rose Bengal Plate test and confirmed by Complement fixation test, respectively. After adjustment for test sensitivities and specificities accordingly, and chi square analysis of results, th...

 

Shakir Ullah*, Aish Muhammad*, Iqbal Hussian*, Hafeez-Ur-Rahman**, Muhammad Zeeshan Hyder***, Muhammad Din**** and Nizamud Din

MORPHOLOGICAL VARIATIONS IN APRICOT CULTIVARS GROWN IN GILGIT BALTISTAN PAKISTAN
... belongs to the family Rosacea, genus Prunus L and the section Armeniaca (Lam). Twelve local
apricot cultivars (Marghulam, Shakarfo, Shakanda, Charmagzi, Shai
Pawand, Nili Pawand, Halman, Habi, Ali Shah Kakas, Astore 1, Narie and
Skardu Local) were evaluated for morphological UPOV (International Union
for the Protection of New Varieties of Plants) and the International Board for
Plant Genetic Resource...

 Asif ullah Khan*, Faizan Ullah*, Sultan Mehmood*, Muhammad Irshad* and Farhat Ullah Khan**

ALLELOPATHIC EFFECTS OF JATROPHA CURCAS L. LEAF AQUEOUS EXTRACT ON EARLY SEEDLING GROWTH OF L. PARTHENIUM HYSTEROPHORUS
...n. Therefore, it is necessary to investigate the interaction of not only with crop plants but also with weed species before its introduction into agroforestry systems. During present investigation, allelopathic potential of leaf aqueous extract was investigated on seed germination and seedling development of L. Extracts were applied at 25%, 20%, 15%, 10% and 5% as seed soaking for 8 hours prior to sowing. Phenolics compounds were found in aqueous extracts and ...

 Tassadduq Rasool*, Ali Zohaib*, Ehsanullah*, Riaz Ahmad*, Tasawer Abbas*, Tahira Tabassum*, Muhammad Ather Nadeem*, Mahmood-ul-Hassan*

FORAGE YIELD AND QUALITY IN PEARL MILLET-SESBANIA INTERCROPPING SYSTEM UNDER VARIOUS GEOMETRICAL PATTERNS
...rsity of Agriculture, Faisalabad. Sesbania intercropping reduced pearl millet growth. Nonetheless, least decrease in height (23%), leaf area (42%) and number of leaves (16%) of pearl millet was occurred by intercropping sesbania in 45 cm apart two-row strips of pearl millet, as compared to sole cropping of pearl millet. Total green forage yield (60%) was increased by sesbania intercropping over sole-cropping of pearl millet and the intercropping of sesbania in...

 Nasarullah*, Raiz uddin*, Fakhar Uddin* and Muhammad Jamal*

INTERPRETING WHEAT GENOTYPES FOR GRAIN YIELD UNDER DIFFERENT REGIMES OF KHYBER PAKHTUNKHWA
...ese traits. Genotypes Faisalabad-2008 and line from Tajikistan (2S-3094) performed better for most of yield and associated traits across both environments therefore could be an asset for plant breeders to breed genotypes suitable for diverse environments.

...
Muhammad Sharif1*, Shahzada Sohail Ijaz2, Muhammad Ansar3, Ijaz Ahmad4 and Syed Abdul Sadiq5
...yield were statistically same under CT (83 plants m-2, 6.02 Mg ha-1, 3.32 Mg ha-1, respectively), MT (83 plants m-2, 5.90Mg ha-1, 3.26 Mg ha-1, respectively) and RT(72 plants m-2, 5.92 Mg ha-1,3.20Mg ha-1, respectively)tillage systemswith retention of crop residues,whilesignificantly lower values were recorded under ZT without residue return (54 plants m-2
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...ogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
...
Youbao Zhong1, Xianlai Zhang1, Xiaofen Hu1 and Yong Li1, 2,*
...er in the jejunal submucosa, but the muscularis was thicker. The intestinal glands were greatly developed in the ileum, lymphoid nodules were observed in the mucosa, and diffuse lymphoid tissue was distributed in the lamina propria. Meanwhile, goblet cells gradually increased from the duodenum to the ileum. The mast cells showed various shapes and sizes in the small intestine and could be detected at four layered structures ...

Samina Ashraf1*, Ghulam Haider2 and Maimoona Ashraf

...at usually people with disabilities and specifically women with disabilities are at increased risk of physical, sexual, and emotional abuse as well as to other practices of violence, such as institutional violence, drug use, undesirable sterilisation, medical abuse, mortification, and nuisance. This study is intended to explore the lived experiences of w...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Zafarullah Baloch3 

...stan, Pakistan. Snowball sampling technique was used to select 90 respondents. The data revealed that average age of the farmers was 53.9 years with education from secondary to graduate level. The results of the study showed that many of the farmers on average have 10 acres of land under date palm cultivation with more than 57 date palm trees per acre on average. It is also observed that respondents generally get 76 kg of dates on average per tree. Many of the...
Muhammad Abu Bakar1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Muhammad Arshad1, Rashid Mahmood2,* and Ziyad Abdul Qadir2
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1Nisar Ahmad3, Ayesha Hassan1, Muhammad Kashif Prince1, Muhammad Luqman Sohail4,*, Mian Saeed Sarwar2, Hazrat Ali2, Tahir Usman2, Asadullah Khan2, Siffat Ullah Khan3 and Shahid Zaman5
... hundred and sixty fecal samples (n=360) were collected through convenient sampling from apparently healthy lambs and were examined using the modified Ziehl Neelsen acid fast staining. Prevalence was calculated along with different risk factors associated with the disease (month, age, sex and season). Results showed highest prevalence (P < 0.05) of the Cryptosporidium in Kohat (33.33%) followed by Lakki Marwat (25%...
Aitezaz Ahsan1*, Muhammad Usman1, Ihsan Ullah2, Aamer Bin Zahur3 and Adnan Rasheed Malik4
...PPR various diagnostic assays, both serological and molecular, have been developed and are applied for estimating the current status of the disease. This review article focuses on the development of different molecular diagnostic assays from conventional reverse transcriptase polymerase chain reaction to real time reverse transcriptase polymerase chain reaction and reverse transcriptase loop mediated isothermal amplification...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
... TR50 showed a promising safety profile when compared to other acaricides, as it spared predatory mites at concentrations which had a high lethality to tomato russet mites. Hence, Huwa-San TR50 can be utilized in the Integrated Pest Management (IPM) program as an acaricide agent.
...
Yanxiao Meng1, Guihua Wang1, Dongmei Xiong1,*, Haixia Liu1, Xiaolin Liu1, Lixin Wang1 and Jianlu Zhang2
...is (DFA) showed that all samples (except six) were correctly reclassified. Our morphological analysis supported the validity of taxonomic status of B. lenok and B. tumensis as two species, and B. lenok tsinlingensis could be considered as an independent species.
...

Irfan Ullah, Shahid Ali*, Muhammad Fayaz and Abbas Ullah Jan 

...er farms in district Charsadda, Khyber Pakhtunkhwa, Pakistan. Data from 120 respondents was collected through multistage random sampling technique. Stochastic frontier cost function of Cobb- Douglas type was used for the estimation of allocative efficiency. The function was estimated using Maximum Likelihood estimation technique. Results revealed that the mean allocative efficiency of broiler farms was 0.84; implies that an ...
Muhammad Zeeshan Majeed1,2,*, Abu Bakar Muhammad Raza2, Muhammad Afzal2,Hafiz Salah-ud-Din2, Imtiaz Sarwar2, Muhammad Yahya2 and Khurram Shehzad3
...vertebrate fauna. Random sampling was done in winter 2015 and summer 2016 using monoliths and pitfall traps. Results revealed that land-used types exert a differential impact on the diversity and population abundance of macrofaunal communities. Highest indices of macroinvertebrate diversity, richness and evenness were exhibited by Lychee (2.39, 12.00 and 1.00, respectively) among cultivated and grassland (2.44, 13.00 and 0.95, respectively) among natural land-...
Fatmagün Aydin1,* and Fatma Burcu Harmantepe2
...cts of dietary levels of sage (Salvia officinalis L.) oil on growth, haematological parameters were evaluated on tilapia (Oreochromis niloticus). For this experiment, 360 fish (11.81± 0.02 g) were used. Fish were divided into four equal groups in three replicates and were fed with diets containing 0, 0.25, 0.5 and 1 % of sage oil for 30 days. The results showed that the haematological parameters (total r...
 Nazia Qamar1, Sher Khan Panhwar1,* and Ralf Riedel2,*
...ic regression, using the same independent variables as above. Prey importance was also investigated. Prey importance was assessed using a Wilcox Rank Correlation analysis on the Index of Relative Importance (IRI) by species and life-stage. Permanova analysis showed that fish was the most important dietary item for juveniles and adults. Adults secondarily preferred crustaceans. Fish was predominant for S. commersonnianus and crustaceans, especially of th...
Burcu Onuk1,*, Murat Kabak1, Bünyamin Sahin2 and Nazan Gezer İnce3

 

...he rostral part of the nasal cavity is cooler to prevent the loss of water that is crucial for the fluid balance of birds. This function may be related to the structure of the nose of flying birds. For this reason, we decided to analyze the nasal structure of two different birds using volume and volume fraction approach of the stereology. In this study, 14 seagulls and 7 storks were examined and volume fractions of the na
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
...broilers were fed with basal diets (CON group), basal diets supplemented with 10 or 50 mg/kg natamycin (T1 or T2 group) for 42d, respectively. The results showed that alkaline phosphatase in T2 group was significantly decreased at 21d, blood urea nitrogen and alanine aminotransferase were significantly increased compared to CON group at 42d in broilers. Serum globulin content (21d) and total protein content (42d) were lower ...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...e, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover, S. dysenteriae IM showed elevated alkaline-phosphatase, leucine arylamidase, trypsin, and acidic-phosphatase activities. The present study reveals that, the API-ZYM test is the convenient, reliable and inclusive alternative to recent biological methods. This method could be applied for the identification of various enzymes on routine bas...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Ali Murad Jakhar3, Rehana Kanwal Rahoo3
...red in relation to the dosage of infective juveniles (IJ) applied and exposure periods. Significantly (P< 0.001) greater numbers of nematodes (predominantly IJ) were recovered from the cadavers of T. molitor that had been inoculated with S. feltiae than H. bacteriophora. The inoculum dose also had an influence on the numbers of nematodes recovered. There were significantly greater numbers of S. feltiae in the 50 and 500 IJ treatm...
Saba Parveen Samo1, Moolchand Malhi1,*, Javed Gadahi2, Yan Lei3, Allah Bux Kaciwal1 and Saeed Ahmed Soomro1
...two groups (n=5/each). Basal diet given to the animals consist of roughage and concentrate at the ratio of 65:35. Group A was kept without any supplementation whereas group B was supplemented with selenium yeast (SY) at the dose rate of 0.3 mg/kg.diet for 8 weeks. The results showed that the weights (% of empty body weight) of rumen (4.18 ± 0.30 vs 3.49 ± 0.10); duodenum (0.32 ± 0.01 vs 0.27 ± 0.01); colon (1.14 ± 0.03 vs 1.0...
Aqsa Gulzar1, Tariq Mahmud1,*, Rubina Munir2 and Asma Anjum3
...oved chemiluminescence assay, respectively. The novel Schiff bases 4a and 4b have been synthesized by the reaction of 2-[(1,3-benzothiazol-2-yl)sulfanyl]-N-[4-(hydrazinecarbonyl)phenyl]acetamide with 3-nitro and 4-nitrobenzaldehyde. Characterization of the synthesized compounds was done by using various spectroscopic techniques namely FTIR, proton and carbon-13 NMR, Mass spectrometry and elemental analysis. The results were in great consistency with the...
Ewa Czerniawska-Piątkowska1,*, Magdalena Skibicka1, Barbara Cioch-Szklarz1, Jolanta Karakulska2, Karol Fijałkowski2 and Sonia Hiller3
...l flora taken from the nasal cavity of animals. The study was performed on 28 calves of Polish Holstein-Friesian Black-and-White variety (PHF HO). The average weight of calves at the age of three months differed depending on the housing system. Individuals in igloo type booths were heavier as compared to the traditionally reared calves. The differences regarding the final weight of heifer calves were statistically significant (P ≤ 0.05). In the cold and tra...
Hao Zhang,1,2,3,* Hai Tao Nie,3 Tie Wei Ma,3 Zi Yu Wang3 and Feng Wang3
...d, ground, mixed, and subsampled for chemical analyses. The Ca, P, Na, and Mg were mainly distributed in bone, except for K, which was mainly distributed in the muscle tissues. The net macromineral requirements for maintenance were 20.20 mg Ca, 13.50 mg P, 3.80 mg Na, 7.91 mg K, and 1.10 mg Mg/kg empty body weight (EBW) for ewes. The net requirements for growth ranged from 11.79 to 12.06 g Ca, 6.04 to 6.16 g P, 1.66 to 1.74 g K, 0.40 to 0.42 g Mg, and 0.98 to ...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...NA extraction from blood samples, a 1486 bp fragment of exon three was amplified and sequenced. Sequence analysis revealed single nucleotide polymorphism (T>A at position 483) and a sheterozygous condition (T>W at position 483). Generalized linear model was used to minimize the environmental effects. The variation i.e. “W” type heterozygous condition showed a higher average daily weight gain. Sequence analysis of LEP gene confirmed the...
Grace C. Onyishi, Ifeanyi Oscar N. Aguzie, Christopher D. Nwani*, R.N.N. Obiezue and I.C. Okoye
...nfected in the hospitals sampled (p < 0.01). Peak abundance of Anopheles mosquitoes was in July and lowest in January. Relative humidity increased from January to July while temperature declined in same direction. Malaria prevalence correlated positively with Anopheles mosquito abundance (r = 0.863, p = 0.012). Mosquito abundance and malaria prevalence strongly correlated negatively with temperature (r = -0....
Wei Dang1, Ning Xu1, Wen Zhang1, Jing Gao2, Handong Fan1 and Hongliang Lu 1,*
...ent temperature were the same. The expression of Hsp70 was higher in the muscles. In liver, Hsp70 expression significantly increased after 38°C heat shock. We then collected other three lizard species, Plestiodon chinensis, Sphenomorphus indicus, and Scincella modesta, from geographical locations near each other, but with different microhabitats. We observed considerable variation in Hsp70 expr...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...). Concentrations of eicosapentaenoic acid and docosahexaenoic were higher in male muscle than in female muscle. Opposite results were obtained with calipash. The n-3-to-n-6 polyunsaturated fatty acid ratios were within the recommended range in all three strains. Chinese soft-shelled turtle is a healthy food source to humans.
...
Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*
...(CRD) in poultry and pheasantry birds of Northern Pakistan is underreported. In this study we report on the seroprevalence and isolation of MG from a total of 2341 poultry and 24 pheasantry birds. Overall, seropositivity of 46.56% (1090/2341) and 27.2% (6/24) against MG was observed among poultry- and pheasantry- birds, respectively. Broiler breeder and broiler showed 58.93% (264/448) and ...
Nilgün Paksoy1, Hikmet Dinç2 and Serap Kılıç Altun3,*
...ey, goat, and sheep milk samples. Fifty-six individual milk samples were collected from 17 lactating donkeys, 19 goats, and 20 sheeps in three different Turkish Farms. The samples were analyzed by inductively coupled plasma - mass spectrometry (ICP-MS). Minimum and maximum levels of Na, Mg, P, K, Ca, Fe, Cu, Zn in donkey milk samples were 108.6-202.7, 48...

Muhammad Jamal Khan1, Abdul Malik2*, Muhammad Shahzad Khattak2, Naveedullah1, Naeem Ijaz3 and Ishaq Ahmad4 

... wells discharges of the sampled tube wells varied from 2 to 14 l/s and the horse power of the pumping units ranged from 2 to 30 hp and the pumps efficiencies of the wells ranged from 22 to 73% with an overall average of 42%. The number of hours the farmers operates their well ranged from 2 to 10 hours per day. The water quality reveals that pH of the water varies from 8.2-8.9 while ECw ranges from 0.4-4.7 dS/m. Out of 36, only inseven villages groundwater has...

Ejaz Ashraf1*, Hafiz Khuram Sharjeel1, Raheel Babar2, Muhammad Junaid1, Qamar Iqbal3, Rabia Rasheed1 and Nosheen Fatima1 

...operational area to give satisfactory coverage, less use of multimedia, unavailability of training facilities to EFS, manifold duties assigned to extension officers and top-down dimension of planning of implementation of extension approaches were main weaknesses. The steps like effective evaluation of extension system, use of democratic nature extension approaches, and training facilities to EFS could improve working efficiency of Extension Field Staff and ext...
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
... Kashmir Pakistan. Total sample size for this survey was consisted of 1,036 lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among mastitis cases the prevalence of clinical mastitis was 10.2% (106/1036 animals) as a whole. However, the prevalence of clinical mastitis in district Lahore was 11.8% (71/598 animals) whereas it was 8% (35/438 animals) i...
Sana Zahra
...56C was identified as causative mutation by Sanger Sequencing. A known missense mutation p.Y556C in exon 15 of SLC26A4 gene was identified in all the four families. It may be concluded that p.Y556C mutation is a recurrent mutation in Punjab province of Pakistan.
...
Lili Zhang, Yuanxiao Wang, Yili Kong, Hussain Ahmad, Rui Yan, Li Dong, Jingfei Zhang and Tian Wang*
...rth were procured. Blood samples were collected at 28, 66, and 160 days of age from both groups. Longissimus dorsi muscle samples were collected from four selected pigs at 160 days of age. Body weight, ADG and ADFI were decreased significantly by IUGR from birth to 160 days of age (P<0.05). At 28 and 160 days of age, the serum urea nitrogen levels of the IUGR pigs were significantly higher than the NBW pigs (P<0...
Mubashar Hussain, Muhammad Faheem Malik, Sehreen Siddique, Muhammad Umar, Tamkeen Zainab, Fatima Zafar
...eoptera: Coccinellidae), sampleswere collected from rainfed and irrigated fields of Gujrat, Punjab, Pakistan during 2015-2016. Adult specimens were collected fortnightly from crops, weeds and shrubs from randomly selected locations i.e. Dolat Nagar, Lala Musa, Jalalpur Jatan, Guliyana, Fateh Pur and Kunjah. Analysis of specimens indicated a representation of 14 species, 6 genera and 3 sub families across all sites. Maximum a...
Humaira Jabeen and Nazia Jamil*
...he desert and urban soil samples. A total of 39 strains isolated from Cholistan near Bahawalpur and 24 strains were isolated from various areas of Lahore. Bacterial strains were screened for the production and accumulation of bio-plastic. Only three strains from urban soil sample were able to produce polyhydroxyalkanoates (PHA). Strain Acinetobacter seohaensis produced 40.6% bio-plastic when supplemented with coconut ...
Fouzia Tabssum1* and Syed Shahid Ali2
... that produce pectinase, samples were collected from the soil, water, fruits and vegetables wastes. Initial screening of pectinase producing bacteria was carried out on pectin containing agar plates of pH 7 incubated at 37ºC. Among isolated thirty-seven strains, a total of thirteen strains showed satisfactory pectinase productivity. Pectinase production of the thirteen strains was then determined qualitatively as well a...
Fouzia Tabssum1* and Syed Shahid Ali2
... that produce pectinase, samples were collected from the soil, water, fruits and vegetables wastes. Initial screening of pectinase producing bacteria was carried out on pectin containing agar plates of pH 7 incubated at 37ºC. Among isolated thirty-seven strains, a total of thirteen strains showed satisfactory pectinase productivity. Pectinase production of the thirteen strains was then determined qualitatively as well a...

Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4 

...al and Achai). Cows were sampled at environmental temperature 18, 32 and 42 °C in February, April and June, respectively. The physiological parameters recorded were rectal temperature (RT), respiration rate (RR) and pulse rate (PR) whereas biochemical parameters determined from blood serum were glucose, cortisol and Heat shock protein-70 (HSP-70). Thermal stress increased all physiological parameterssignificantly (P < 0.001). Holstein Frisian manifested...
Muhammad Tariq Zahid1, Farah Rauf Shakoori2, Soumble Zulfiqar3, Khalid A. Al-Ghanim4 and Abdul Rauf Shakoori3,4*
...eat grain medium, Bold-basal salt medium and modified Neff’s medium, respectively. T. farahensis exhibited significant copper storage ability removing 54.9 % copper from medium within 96 h of 30 µg/ml copper stress. The maximum uptake rate was observed within 30 min of copper administration in response to 5-100 µg Cu++/ml in the medium. Quantitative analysis showed that TfCuMT is a ...
María Magdalena Crosby-Galván1, Bernardino Espinoza-Velasco1 and Mónica Ramirez-Mella2,*
... in an in vitro assay. Four treatments were evaluated: a control using a standard diet for ruminants elaborated with corn stover, corn grain and soybean meal, and three experimental diets with 10, 20 and 30% dry residue of chihua pumpkin instead of corn stover. Dry matter digestibility and gas production was determined at 24 h of incubation in ruminal fluid obtained from two Holstein cows with a ruminal cannula. Randomized block experimental design was ...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...mmended.
...
Tariq Mukhtar,1,* Abdul Jabbar1, Muhammad Usman Raja1 and Humayun Javed2
...hakwal-50 followed by Faisalabad-2008 and Lasani-2008 whereas the minimum incidence was observed on cultivars Punjab-2011 and Millat-2011. No incidence of the nematode was recorded on Shafaq-2006 and Aas-2011 cultivars. As the nematode was found prevalent throughout Sargodha division with quite low incidence, therefore, it is imperative to keep the nematode under constant surveillance and stringent control strategies should ...
Nazish Imran 
...acity of societies to be safe and productive”.(2) More than half of all mental disorders have an onset in childhood and adolescence with suicide being the third leading cause of death among adolescents.(1), (3) Child & adolescent mental health thus needs to be considered & emphasized as an integral component of overall health & growth of young populat...
Shagufta Andleeb*1, Shabana Shaukat1, Chaman Ara2
...ole of garlic (Allium sativum), to minimize the intensity of these toxicities. For this purpose, fertilized eggs of Gallus domesticus were randomly divided into four groups of forty eggs each. Control group was intact and untreated. Eggs of one group were injected with a sub-lethal dose of cadmium chloride (1.5 µg/egg) in albumin on 7th day of incubation. In another group, eggs were treated with cadmium chloride and fresh garlic ...

Muhammad Suleman Bacha1*, Mohammad Nafees1 and Syed Adnan2
 

...onnaires administered to sample households and 9 FGDs (Focus Group Discussions) in selected villages with the aim to understand local perceptions about changing climate, its vulnerabilities and farmers adaptive measures toward climate change in district Swat of Khyber Pakhtunkhwa, Pakistan. Additionally, vulnerability matrix was used to identify livelihood resources that are vulnerable to climate change induced hazards. Results showed that primarily deforesta...
Riyadh S. Aljumaah*, Mohammed A. Alshaikh, Abdulrahman Jarelnabi, Mutassim M. Abdelrahman and Mansour F. Hussein
...e linked immunosorbent assay (ELISA). All camels tested were clinically normal. Out of 253 camels of either sex, 117 (21.99%) were found to be serologically positive to specific anti-N.caninum antibodies. Logistic regression analysis and estimation of odds ratio revealed significant association of the sex and location of the camels with N. caninum seropositivity. On the other...
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
...n sheep. Total 600 blood samples were taken each 200 from districts Bannu, Tank and Dera Ismail Khan of southern Khyber Pakhtunkhwa Pakistan and screened through blood microscopy. The current study revealed an overall prevalence of ovine theileriosis as 27.5%, 13% and 19.5% in districts of Dera Ismail Khan, Tank and Bannu, respectively. Sex, tick infestation, animal keeping conditions, herd size, season and feeding pattern were observed as significant factors ...
Song Jiang1,2, Fa-lin Zhou3, Jian-hua Huang1, Qi-bin Yang1, Li-shi Yang1 and Shi-gui Jiang1,*
... 27.0±1.5 °C, salinity of 30–32, pH of 8.0–8.3, and light intensity of 1000 lx. Results showed that food consumption and feeding intensity of the eyestalk-ablated broodstock were higher than those without eyestalk ablation. The night-time feeding intensity was 1.46-fold and 1.31-fold higher than the daytime feeding intensity in the treatment and control groups, respectively (P<0.01). Food consumption in the treatment group incr...
Farhan Ahmad1,*, Muhammad Waris Sanjrani1, Shah Nawaz Khuhro1, Asif Sajjad2, Abid Ali3, Rashad Rasool Khan3, Farooq Ahmed3 and Junhe Liu4,*
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...nd can recognize lipopolysaccharide (LPS), the main component of gram-negative bacteria. To investigate how TLR4 responds to LPS, the TLR4 transcription levels in duck liver, spleen and bursa of Fabricius were examined after a model for immune stress was implemented through LPS injection in Peking ducks. The results from this assay suggested that LPS injection significantly increase...
Sherin Reda Rouby
... from different clinical samples and differentiate capripoxviruses without the need of gene sequencing. A total of 16 lumpy skin disease clinical samples (skin nodules, scabs, buffy coat, lymph aspirate and engorged ticks) and three sheep pox biopsy skin samples were subjected to DNA extraction followed by 30 kDa RNA polymerase subunit gene-based polymerase chain reaction (PCR) (RPO30...
Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*
...here were 5 RVF positive samples (5.15%) in 2 broilers, 1 laying hen and 2 breeders. Only one RVG sample (1.03%) was detected in laying hen, in coinfection with RVF. This data contributes to a better understanding of rotavirus distribution in Brazil.
...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...rs of 2015 and 2016. All samples were screened using virus isolation, as well as antigenic and molecular detection methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...ion. In total, 146 serum samples from 47 individuals were analyzed. Serum neutralization test was used to determine presence of CDV antibodies, and an indirect IgG ELISA was used to detect CPV antibodies. Neutralizing antibodies was induced after CDV vaccination and persisted up to 3.9 years. Most animals had high IgG antibody titers to CPV prior to vaccination and vaccination did not resu...

Muhammad Suhaib1, Ijaz Ahmad2*, Masooma Munir3, Muhammad Bilal Iqbal4 , Muhammad Khubaib Abuzar5 and Safdar Ali6 

...xt-align: justify;">Soil salinity is a menace that is affecting the agricultural soils and turning these soils into low productive ones and interfere the normal crop development. Wheat is an important cereal crop and salinity is also affecting the growth and yield of this crop. While salicylic acid is found to play a helpful role in supporting the plant growth and development under
Muzafar Shah1,*, Mazhar Ali2, Sardar Azhar Mehmood2, Shabir Ahmad2, Khushi Muhammad3, Israr Alam1, Kausar Saeed1
...oncluded that out of 997 samples, 204 (20.46%) were found positive, in which mostly under positive, under 15 year of age (23.36%), whereas less cases were recorded in adult (46 and above) 22.64. Most of the positive cases were found in the month of November, 24 out of 67 (35.82%) and less cases were noted in the month of May which are 12 out of 106 (11.32%). Incidence of the cases were found in U.C Kota with 60 positive (26.08%) out of 230 blood
Waqas Ali1,*, Arshad Javid1, Syed Mohsin Bhukhari1, Ali Hussain1, Syed Makhdoom Hussain2, Hira Rafique1
...nd management. Different sampling techniques are applied during herpetofaunal surveys and some of them might be expensive, time intensive and their effectiveness in different regions of the world are still debatable. Moreover, data recorded from inappropriate designed surveys are not suitable for statistical analysis and provide inappropriate picture regarding distribution, abundance and status of target species. Current review inspects different
Muhammad Umar1, Mubashar Hussain1,*, Ghulam Murtaza1, Farid Asif Shaheen2 , Fatima Zafar1
...iewed extensively. The disappearing trends of migratory birds are recorded from fresh water reservoirs of Pakistan and are mainly attributed to continued loss of wintering habitat, habitat modification, fragmentation, eutrophication, anthropogenic activities and illegal hunting. The adverse effects of habitat loss (Band tailed fish eagle, Black Stork and Water Pipit), pesticides (Gulls, Eagles, Terns, Ducks and Cormorants), eutrophication (Painted stork and Da...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...y ensures the effective usage of lignocellulosic biomass at large scale biofuel production. 
...
Huma Ayub1,Iftikhar Ahmad2, Syed Lal Shah3,*, Muhammad Zafarullah Bhatti4, Nuzhat Shafi5 and Mazhar Qayyum1
...ne 2014 to May 2015. The samples were collected from three partially separated stations of the Chashma Lake on a monthly basis. A total 42 species of zooplankton were identified from the Chashma Lake belonging to three taxa viz.; copepoda, rotifera and cladocera. The diversity of the zooplankton species in the lake was determined by richness and evenness indices, which were found as follows; Margalef Index 1.30 to 3.87, Shannon’s Index 2.54 to 3.6...
Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...Pakhtunkhwa (KPK). Blood samples from 900 individuals of various age groups were collected (August to November 2013) from patients at clinics, houses and pharmacies belonging to three Tehsils (Puran, Alpuri and Besham) of district Shangla. The Immuno-chromatographic (ICT) and enzyme linked Immunosorbant Assay (ELISA) techniques were applied for the prese...
Hamayun Khan1,*, Abdul Wahab1, Navaid Kazi2, Younas Muhammad1,Summaya Kazi3, Salman Kazi4, Azmatullah Khan1, Ikramullah Khan5 and Shakoor Ahmad1
...rogen (BUN), alanine transaminase (ALT) and aspartate transaminase (AST). The current study demonstrated the ascertained value in amniotic fluid for glucose, total protein and albumin at day 18, 25 and 28 was 42.9±0.80, 37.4±0.90, 36.5±0.88mg/dl; 17.9±0.30, 16.1±0.22, 13.9±0.34g/l; 13.9±1.6,11.5±0.75, 8.8±0.15g/I whereas that of creatinine, triglyceride, BUN, ALT...

Nihal Ahmad Hadi, Shahid Ali* and Umer Wahid

 

... Pakistan. A multi-stage sampling technique was used to collect data from 150 broiler farmers. A well-structured interview schedule was used with both close and open-ended questions. Data from broiler farmers were collected during April and May 2016. Maximum Likelihood Estimation (MLE) technique was used for estimation of stochastic frontier Cobb-Douglas type production function. Results indicated the mean technical efficiency of 0.89 with the range of 0.63 to...

Shahid Ali1* and Salamat Ali2  

...ab, Pakistan. Multistage sampling technique was applied for selection of sampled respondents. 120 farmers, 60 farmers of open shed and 60 farmers of EC shed were interviewed during 2014. Dummy variable regression was applied for estimation and comparison of costs and revenues of open shed and EC shed broiler farms. Results revealed that there was statistically significant difference between the cost of production and net rev...

Habibullah* and Sahib Alam  

... wheat crop grown on the same plots. The soil physicochemical properties were evaluated in the beginning, reproductive stage and at maturity of each crop. The average values of soil pH, electrical conductivity (EC), organic matter (OM), N, P and K of the soil before sowing of maize crop were found to be 8.2, 0.37 dS/m, 0.95%, 1.74 mg/kg, 3.45 mg/kg, 61.5 mg/kg, respectively. Biochar and N application significantly (p≤0.05) increased soil pH whereas soil OM ...
Kashifa Naghma Waheed1,2,*, Zaid Mehmood2 and Sikender Hayat1
...It was observed that the samples contained potential amounts of essential elements, while the toxic elements were present in low quantities. The results were statistically analyzed and compared for their significance and showed significantly higher quantities of Ca, Na, K, Fe, Mg followed by relatively lower quantities of Al, Mn and low quantities of Zn, Co, Ba, Li, Pb, Cu, Sr, Ni, Cr, La and Cd. It was concluded that this naturally fortified by-product of sug...

Nadia Faqir1, Aish Muhammad2*, Ghulam Muhammad Ali2, Armghan Shehzad2, Hafeez Ur Rahman3, Farhatullah4 and Muhammad Zeeshan Hyder

...ng the studied date palm samples pertaining to the studied physical traits and chemical composition of fruit. Sequences of the maturase k gene has complete identity to the reference genome of ‘Khalas’ cultivar. While geranyl geranyl biphosphate reductase has one synonymous single nucleotide polymorphism in Qantar, Hamin wali, Kupra and Shakri, suggesting a lack of divergence in these genes in the studied cultivars. Tree plots generated showed incon...

Murad Khan1* and Muhammad Afzal2 

...tured questionnaire. The sample farms were categorized into three groups i.e. small, medium and large. For analysing the data, profit function and multiple regression model, analysis of variance (ANOVA) and independent t test were used. The average profit of large size farm was Rs. 85228 followed by medium and small farm that were Rs. 58049 and Rs. 36090 respectively. The results of independent sample t-test indicated that t...
Leila A. Kaimbayeva1,*, Elena S. Malysheva2, Rashit Kazikhanov3 and Saule R. Kazikhanova4
...For these purposes, meat samples were collected from quadriceps of 2.5-4 years old female red deer, immediately processed for isolation of muscular fibers and were evaluated for the pH, water-binding power, activities of tissue enzymes and microstructure changes. The results of chemical analysis revealed the nature of protein substances, altered рН value of the meat and the intensity of glycolysis. All these parameters were indicative of autolysis ...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...ember 2013, in total 700 samples were collected from different species of non-vaccinated birds inhabiting diverse ecological zones of Pakistan. Altogether, 507 tissue and swab samples were screened for the presence of viral RNA by reverse transcriptase polymerase chain reaction (RT-PCR). Same samples were further processed for the isolation of virus by embryonated egg inoculation technique...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
...e future.
...

Mohammad Javad Golmohammadi1, Hamid Reza Mohammaddoust Chamanabad1*, Bijan Yaghoubi2 and Mostafa Oveisi

...relative cover (RC), the samples were collected from 481 fields during the growing season and 255 fields after harvesting rice in Guilan province (Iran) from 2014 to 2016 (from April to March of each year) based on the method of Thomas by throwing 0.5m × 0.5m quadrat to count existing genus and species of weeds. According to the results, the highest frequency was related to weed species of Echinochloa crusgalli (96.9%), Paspalum distichum (78.8%) and Ec...

Muhammad Waqar Yasin*, Mobushir Riaz Khan and Muhammad Amin 

... be trained to take necessary precautions while looking after their crops at different stages of crop cycle regarding these climatic variables i.e. temperature and rainfall. 

...

Mahwish Rehman1*, Sajjad Ahmad1, Maqsood Shah1 and Inamullah Khan

...istle hair, stiff) on dorsal shield, chaetotaxy and structure of the sternal, , metapodal, anal, and epigynal shields, periterme, morphology of setae on the legs and palps and its arrangement and number were observed. Results of the present study showed that the studied specimens were Varroa destructor, however, no Varrova jacobsoni were recorded during chaetotaxy and morphological studied. The study also confirms that V. jacobsoni population is not present in...
Shuai Shang1,2, Honghai Zhang2,*, Xiaoyang Wu2, Qinguo Wei2, Jun Chen1, Huanxin Zhang1, Huaming Zhong2 and Xuexi Tang1,*
... bitter, umami, sour and salty. Among these tastes, the bitter sense is believed to help animals identify poisonous compounds. Bitter taste receptors coded by bitter taste receptor (Tas2r) genes has been identified in many species, but the evolution and repertoire of Tas2r genes in raccoon dog (Nyctereutes procyonoides) was still unknown. As a true omnivorous mammal, the Tas2r genes may play a critical role in food selection of racc...

Abdur Rehman1*, Wang Jian2, Noor Khan3 and Raheel Saqib

...cteristics belong to the same level of market risk. Therefore it is essential to accomplish the market risk management system to monitor the risk of major crops by introducing various kinds of products. This ensures the competency, accuracy and decreases significantly the cost of market risk management.  

...

Sher Nawab Khan* and Ghulam Hassa

...), Punjab-11 (PJ-11), Pirsabak-2005 (PS-05), Pirsabak-2013 (PS-13), Janbaz (JB) and land race Khatakwal (KW) were crossed in 8×8 full diallel pattern to identify aphids resistant genotypes under irrigated and rainfed conditions. Number of aphids tiller-1 revealed highly significant differences (P ≤ 0.01) among the genotypes. Under irrigated condition genotypes PJ-11×Lal-13, Tat-96×Lal-13, JB×Lal-13...

Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Muhammad Jamal Khan1, Zahir Shah1 and Farmanullah Khan1 

...ge. In early stages, the salinity affects the germination and emergence of the young seedlings and then the growth and yield of various crops and ultimately disturbing the socioeconomics of the farming community. Among the microbes, cyanobacteria are known as photoautotrophic, blue green algae which is not a truly eukaryotic algae and grown in different and such complex habitats freely and symbiotic association with large numbers of plants and microbial mats. ...

Imran Khan1, Zahir Shah2, Wiqar Ahmad3*, Farmanullah Khan2 and Muhammad Sharif

...t, unless otherwise necessary, deep tillage in alkaline calcareous soil should be avoided, preventing lime resurfacing from depth and keeping the applied amendments concentrated in surface layer. 

...
Muhammad Khalid Mukhtar1,*, Hummaira Iqbal1, Hafiz Muhammad Tahir2, Haseena Ghulam Muhammad1 and Muhammad Irfan1
Ghulam Muhae-ud-Din1,4, Anam Moosa1, Umer Farooq Ghummen1, Muhammad Jabran1, Amjad Abbas1, Muhammad Naveed2, Abdul Jabbar1 and Muhammad Amjad Ali1,3,*
...ant, Cordyline fruticosa, Hibiscus syriacus and Carrisa macrocarpa were moderately susceptible, Murraya paniculata and Ficus benjamina were susceptible, F. elastica, Artemissia stelleriana and Duranta repens were highly susceptible to M. incognita infection according to a well established 0-4 galling scale.  The highest galling index and reproductivity of ne...

Muhammad Suhaib1*, Ijaz Ahmad2, Masooma Munir3, Badar-Uz-Zaman1, Bushra Atta4 and Muhammad Khubaib Abuzar5 

...otypes supplemented with salicylic acid (0.25 and 0.5 mM) under salt stress (75 and 150 mM). It’s an obvious factor that under salt stress certain reactive oxygen species are produced that damage the vital plant organelles causing disturbances in the normal functioning of the plant and even may lead to death in severe cases. The results of this study conferred that there were genotyp...

Safdar Ali1*, Obaidullah Shafique1, Tariq Mahmood2, Muhammad Amir Hanif1, Ijaz Ahmed3 and Bashir Ahmad Khan

...h and concerns about the safety of nanomaterials.

...
Arshad Mahmood Malik1, Khalid Mahmood Mughal2, Sarfraz Ahmed Mian2 and Atta Ullah Khan2
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...ity as assessed by MTT assay. This study also showed that N-terminal fusion of SUMO withIFNα2-Tα1 is effective for its soluble expression and to make its purification process more convenient.
...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...f bacteria. Among the causative agents of dental caries, notable one is Streptococcus mutans, which is an obligate parasite. In this study eleven S. mutans strains were isolated from carious lesions on Mitis Salivarius Bacitracin agar along with twenty potentially active Streptomyces strains were isolated from the soil of agriculture farmlands. The biochemical and physiological characterization along with the molecular identification by 16...
Hongqun Li1, Xiaoli Liu2,*, Zhenmin Lian3,RenheWang4, Yongbin Wang4, Yongyao Fu1 andDingyi Wang5
...e” brown-eared pheasant (Crossoptilon mantchuricum) to compare microhabitat characteristics of successful and unsuccessful nests in the Huanglongshan Nature Reserve, Huanglong County, Shaanxi Province, China, from 2006 to 2014 except for 2008. Forty (62.5%) of the 64 nests that we monitored were successful. Successful nest sites had greater tree cover, increased cover and density of shrubs, and more low-lying shrub cover (1.0 m in height) than uns...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
...tional study, 186 sputum samples were collected randomly from jail inmates. Sputum was processed for smear microscopy and culture inoculation for the detection of Mycobacterium tuberculosis infection. Moreover, the suspected samples were further investigated by polymerase chain reaction (PCR). Three samples were found positive by PCR test while other detection methods were no...
Muhammad Mohsin Ahsan1, Hafiz Muhammad Tahir2,*, Khizar Samiullah3
...initimus prefers dry sandy habitat with lower vegetation. This information furthers our scientific understanding on the existing record of this scorpion species in Pakistan.
...
Asad Sultan1,*, Shahroom Khan1, Sarzamin Khan1, Naila Chand1, Muhammad Shuaib Khan2 and Hamza Maris3
...FCR) was recorded in the same group. Tibia dry matter, ash, calcium (Ca) and phosphorous (P) were significantly (P<0.05) higher in OA-2. On the other hand, fecal Ca and P decreased significantly (P<0.05) in OA-2. Mean dry matter and ash contents increased significantly (P<0.05) in OA-2, while crude protein, crude fibre, ether extract and metabolizable energy increased significantly (P<0.05) in OA-1.5 and OA-2. In conclusion, addition of OA in drink...

Ashraf Sayed Awaad1 and Mohamed Zaki Fathy2*  

... the anatomy of the lumbosacropelvic region and confirmed by computed tomography (CT) as well as successful application of the needle within the epidural space guided by ultrasonography (US). This study was conducted on seven lumbosacropelvic regions of goat cadavers and five live goats of both sexes ranged between 25-30 kg body weight. Longitudinal and cross anatomical sections were done on these sa...

Saima Rani*, Hassnian Shah, Nusrat Habib and Muhammad Azeem Khan 

...on health from chemical usage, the more likely they would be willing to pay a high price for a fresh organic vegetable. 

...

Ghufran ul Haq1*, Muhammad Arif2, Asad Ali2 and Mian Inayatullah3 

...plains of Shabqadar, Charsadda and Peshawar during 2011, 2012 and 2013, to determine the incidence of TYLCV and the population of its vector. Triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) was successful in detecting TYLCV in naturally and whiteflies inoculated tomato plants. Transmission proper...

Muhammad Adnan Khan1*, Majid Suhail Hashmi1, Ali Muhammad1, Muhammad Muneeb1, Haris Bilal1 and Gul Wali

... ratio respectively. All samples were tested for total soluble solids (TSS), pH, titratable acidity, reducing sugar, non-reducing sugar, ascorbic acid and sensory properties as flavor, color and overall acceptability. Samples were stored for three months and readings were recorded at every 15 days interval. During storage intervals, TSS increased from 48.0 to 51.31°Brix, pH increased 3.82 to 4.25, titratable acidity decreased from 1.02 to 0.86%, reducing s...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...rn in the tested chicken samples, as it was measured to be higher than its maximum permissible limit (0.1 mg/kg) in poultry meat set by WHO and European Union. The estimated daily intakes (EDIs) of the tested metals through the consumption of the Baladi chicken were found to be lower than their respective reference oral doses (RfD) set by USEPA. The non-carcinogenic health risks to the target population due to exposure of the tested metals were assessed by est...
Mehmet Aydın*

 

.../i>and Eriphia verrucosa) living in the Black Sea. The crab samples were obtained between 2010 and 2016 by SCUBA, trawl, trammel net, and dredges at six locations (Kastamonu, Sinop, Samsun, Ordu, Giresun, and Trabzon). Total carapace length (CL) and weight (W) of each individual were recorded with an accuracy of 0.01 cm and 0.01 g, respectively. During the sampling period, a to...

Rehman-Ud-din* and Naeem-Ur-Rehman Khattak 

...he impact of farm mechanisation on wheat and maize crops productivity in Peshawar valley. For this purpose, three districts, i.e. Peshawar, Charsadda and Nowshera in the said valley, were randomly selected followed by a selection of three villages in each district under simple random selection. In this way, a sample size of 175 farmers was randomly selec...
Muhammad Waheed Mushtaq1,*, Jamil Anwar2, Omara Naeem3,Farah Kanwal1 and Waheed-uz-Zaman1
...r complete freezing, the samples were collected from various sections of the frozen mass and bacterial colonies were counted after proper Gram staining. It was found that more than 90% bacteria were separated along with freezing front and were concentrated in the centre in the case of radial freezing and at the bottom in case of vertical freezing.
...
Zeqin Fu, Yunfang Tian, Yingying Ye*, Pengzhi Qi and Changwen Wu
...pairs to screen all microsatellite loci and twenty-three polymorphic loci were identified. The number of alleles per loci ranged from six to twenty-one with an average of 12.913 and the allele size varied between 161 and 470 bp. The observed and expected heterozygosity varied from 0.143 to 1.000 and from 0.351 to 0.926, with average values of 0.614 and 0.771, respectively. Eighteen loci accorded with the Hardy-Weinberg Equilibrium (HWE)(P > 0....
Muhammad Nazar Aftab1, Ahmed Ali1, Muhammad Asad1, Sadia Fatima2 and Furhan Iqbal2,*
...l group was treated with saline solution for the same period of time. Complete blood count were determined in both experimental treatments at the end of dose supplementation. Our results revealed that oral supplementation of 80mg/Kg body weight of AlCl3 for 16 days did not affect (P > 0.05) any of the studied parameters of complete blood count. On the other hand, serological parameters like triglycerides (P = 0...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,* 
...determine specificity of salvia miltiorrhiza (SM) on growth plate width, liver oxidative stress and toxicity in avian TD. One hundred and fifty broiler chicks were equally distributed into three groups: Control, Thiram (50 mg/kg/d) and SM (10 mg/kg/d) treated. Results showed that thiram caused lameness, decrease in alkaline phosphatase (ALP) activity and increase in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the serum alo...

Wiqar Ahmad1* and Farmanullah Khan

...4% and porosity (ƒ) saturation (ω) and available water content (θ) increased by 3.6%, 5% and 11%, respectively, over the RD. The cereal-legume rotation recorded 16, 15 and 32% higher total N, mineral N and soil OM, respectively, whilst it reduced ρ by 3% with increase in ƒ, ω and θ by 3, 11 and 3% over the cereal-cereal rotation, respectively. The study concluded that yield potential and fertility status of eroded soil can...
Jia-kui Li1,2,*, Kun Li2, Zhao-qing Han2, Hui Zhang2, Xiao-qiang Wang2, Hou-qiang Luo2, Yan-fang Lan2 and Gang Qiu1,2
...e-linked immunosorbent assay (ELISA) kit to test the prevalence of BVDV(Bovine Viral Diarrhea Virus) in yaks in Hongyuan, Sichuan province, a cold climate and high altitude area of China. Totally, 516 yak serum samples were randomly got during 2012 to 2014. BVDV was found highly prevalent among the adult yaks (51.1%; 95% CI: 46.5-55.6) and calves (60.5%;...
Nadia Saeed1,Mian Sayed Khan2, Habib Ahmad3, Muhammad Abdullah4 and Muzafar Shah5,*
...he rate of 8 Kg/tree and saw dust at the rate of 10kg/tree, respectively. Untreated trees were kept for the comparison. Soil samples and sub samples of both treated and untreated trees after 3, 6 and 12 month from Abbottabad and Mansehra by using Bearmann funnel technique. After Quantitative analysis of both treated and untreated trees, it was observed that treated trees
Gulnaz Malik1, Abdul Qadir1 and Hafiz Azhar Ali Khan2,*
...icides viz., spinosad, emamectin benzoate, lufenuron and thiamethoxam. For this purpose, toxicities of insecticides were tested at three different temperatures 20°C, 25°C and 30°C, on whole wheat grains which was artificially infested with S. oryzae under laboratory conditions. The toxicities of spinosad, emamectin benzoate, lufenuron and thiamethoxam increased 2.65, 1.59, 1.64 and 3.00 folds...

Xiangbin Meng1,Tianxiang Gao2, Chunhou Li3, Na Song1, Lu Liu1, Liqin Liu2 and Xiumei Zhang1,*

 

...in potential useful microsatellite markers for S. esculenta. Among 80 tested microsatellite markers, 19 showed polymorphism among S. esculenta individuals. The allele number of all polymorphic microsatellite markers ranged from 6 to 10. Expected and observed heterozygosity varied from 0.260 to 0.904 and from 0.292 to 0.861, with an average of 0.741 and 0.607, respectively. Th...
Nausheen Irshad1,*, Imran Yousaf1, Tariq Mahmood2 and Muhammad Saeed Awan1
...ard at six out of twelve sampling sites surveyed, in the form of evidences including pug marks, cave/ den, and dead bodies (two carcasses and one skin) of the animal. Moreover, three kill records of the animal in a short duration of six months are indicative of its illegal hunting for its skin or in self-defense. Therefore, immediate conservation measures are needed to protect the species. The other aspects of leopard ecology like habitat requirement, food and...
Muhammad Asim Khan1,*, Shahid Niaz Khan1, Sanaullah Khan2, Abdul Jabbar Khan3, Muhammad Adnan4, Nawab Ali5 and Ijaz Ali6
...n (Peshawar, Mardan, Charsaddah, Sawabi, Nowshehra) and Southern (Bannu, Lakki, Karrak, Kohat, D.I.Khan) districts of Khyber Pakhtunkhwa, Pakistan. All the films were examined by microscopy for the detection of malarial parasites. In the Southern districts, the overall incidence of Plasmodium slides positivity was 52.47% of which 91.07% were identified as Plasmodium vivax and 6.23% as P. falciparum and 2.69 was 43.62%, of which Plasmodi...
Tariq Mahmood1,*, Shafqat Rasool1, Faraz Akrim1, Shaista Andleeb1, Muhammad Sajid Nadeem2 and Fiaz Nadeem3
...e park at five different sampling sites, at elevations ranging from 615 m to 655 m above sea level. Only one of the three species Tawny owl  was previously known from the park while the other two species have been recorded for the first time from the MHNP. On the contrary, three other owl species that were previously known from the park (Asian Barred Owlet Glaucidium cuculoides, Oriental Scops-owl Otus sunia and Pallid Scops Owl Otus...
Abdul Samad1, Ferhat Abbas1, Zafar Ahmad1, Olena Pokryrshko2 and Tauseef M Asmat1,*
... of 800 most common food samples such as chicken, beef meat, raw milk, vegetables and salad samples were collected from retail market, the overall contamination of food samples with infectious agents was 48.37%. Campylobacter jejuni was recorded much higher (28.99%) compared to other foodborne pathogens. Food sa...
Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
...gene obtained from fecal samples from three cape oryx animals, one female and two males. At the 3% level in our research, we found 3959, 4553, and 3930 operational taxonomical units (OTUs). Additionally, the three samples have 754 OTUs in common, which comprised 19.59%, 16.55%, and 19.81% of the reads in C1, C2 and C3. We identified 18 prokaryotic phyla in these animals, but most of the gut flora belonged to three phyla: Fir...
Hazrat Nabi1, Irshad Hussain2, Muhammad Adil3, Amar Nasir3,*, Arbab Sikandar3, Saeed Khan1 and Nasrullah Khan3

 

...of thymus, spleen and Bursa of fabricius of aflatoxins binders administered groups (groups E and F) were also significant compared with ones fed aflatoxins mixed feed (groups B and C). In conclusion, mycotoxin binders counteracted the deleterious effects of aflatoxins on antibody profile, weight gain, feed consumption and visceral organs of broilers suggesting that these agents can be potential ameliorators against aflatoxicosis in broilers.
...
Tengfei Lu1, Wenhua Pei1, Shuang Zhang2, Yangnan Wu1, Fenghao Chen2, Xiao Han2 and Weijun Guan1,*
... were sub-cultured to passage 25 in vitro. The gene of Oct-4, Rex-1, CD29, CD44, CD71, and CD73 were identified by RT-PCR and immunofluorescence technique. The results showed that they were positive in AMSCs. The growth of different passages cells typically appeared in S curve. Furthermore, AMSCs successfully differentiated into osteoblasts, adipocytes, and hepatocyte-like cells after procedure induction. The results ...

Shamsher Ali1*, Sarmad Khan1, Muhammad Jamal Khan2, Naveed ullah4, Muhammad Rashid3 and Wiqar Ahmad1 

...eties i.e Atta Habib, Barsat, Siren, Pirsabak-2004 and Pirsabak-2005 were cultivated under three soils having different salinity levels; i- Normal-non saline soil (EC=0.9 dS m-1), ii- moderately saline soil (EC=7.2 dS m-1) and iii- highly saline so...

Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair

...d visceral organs. Eight samples were screened positive for the major MDV-encoded oncogene Meq which was subjected for alignment and phylogenetic analysis. The eight studied consensus amino acid sequences of Meq proteins possess several amino acid mutations associated with the MDV virulence and a unique distortion in the Proline repeats (Proline-to-Alanine) at position 176 in the Egyptian MDV strains. The Phylogenetic analysis grouped the eight analysed sequen...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
... be transferred to 34 passages nowadays. Surface marker Oct4, CD105, nanog, CD73 and SSEA-4 on CAFSCs are detected positive with immunofluorescence histochemistry, Gene CD44, CD29, CD73 and SH2 are detected positive with RT-PCR. We carry karyotype analysis out and find that chromosome is 2n=78 and shows normal. CAFSCs in mid period of subculture propagate fastest from cell cycle examination on Flow Cytometer and growth curve using CCK-8 kit on ELIA
Adel A. Basyouny Shahin,1,* Amira M. Metwally Mohamed2 and Abd El-Reheem A. El Shatter2

 

...ere monomorphic with the same allele fixed in all individuals from both karyotype forms and only 7 (41.2%) loci were polymorphic with two different alleles. Levels of genetic variability between the two forms were relatively low and are comparatively either within the range or quite different from those of the same species occurring elsewhere. The means of expected (He) and observed (Ho) h...
Danyu Zhang1, Ying Liu1, Qinxin Shi1, Zhiwei Peng1, Yan Hua1 and Zhijun Hou1,2,*
...t>ble.
...
Ali Zaman1,*, Abdul Haleem1, Sajjad Ur Rahman2, Adnan Amin3 Asma Saeed1, Sana Ullah4 and Naimat Ullah4
...ts of the province. Necessary measures are required to overcome the situation and improve vaccination and hygiene.
...
Wael S. El-Tohamy1,*, Russell R. Hopcroft2 and Nagwa E.M. Abdel Aziz3
... (P< 0.05) related to salinity, temperature, phosphate concentration and phytoplankton biomass. The main spatial gradients along the estuary were associated with salinity. The high salinity zone in the estuary downstream was dominated by the calanoid paracalanidae and the harpacticoid Euterpina acutifrons. In the lower salinity transects, the t...
Xi-wen Chen1,2, Qian Wang1,3, Miao Yin1, Zhong-hui Pu1,2, Ai-wei Guo3, Lian Li 1,3, Wen-tao Luo1,3 and Xiong-qing Wang1,*
...ctical early detection assay is required. This study established a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay to detect PRRSV. Four primers were designed targeting the ORF5 gene of PRRSV were designed. Reverse transcription and amplification of the viral RNA using AMV reverse transcriptase was optimal at a constant temperature of 65 °C. The output of the RT-LAMP as<...
Yang Yang1,2, Yun Liu1, Mingxu Zhou1 and Guoqiang Zhu1,*
...s measured by motility assays, bacterial gene expression, and host responses to infection. The QS systems and flagellin were coordinately regulated, as deleting fliC caused decreased QS-II activity.
...
Syed Makhdoom Hussain1,*, Nosheen Aslam2, Arshad Javid3, Shehzadi Liaquat1,Muhammad Mudassar Shahzad1, 4, Muhammad Zubair-ul-Hassan Arsalan1 and Muhammad Adnan Khalid1
...orded in fish fed on the said test diet. On the basis of these results it was concluded that probiotics supplementation at 3 gKg-1 level in canola meal based diet is helpful for maximum performance of Oreochromis niloticus fingerlings.
...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
Muhammad Shahid Saeed1, Adeela Shahid2, Samia Jawed3, Muhammad Akram4, Irshad Hussain Qureshi5 
...ven placebo (0.9% normal saline). Salbutamol inhalation was given at regular intervals and Spirometry was performed to determine lung functions.
Results: The two groups (study group and placebo-treated group) were compared for FEV1, FEV1% predicted, PEFR and PEFR % predicted. There was 1.06 L increase in FEV1 in study group compared to 0.8 L in placebo group (p<0.05). The mean FEV1 in the s...
Sadaf Noveen1*, Shaista Habib Ullah2, Babar Alam3
...g children without any disability or ignored craniofacial deformities. Studies highlighting this relationship are rare to none in Pakistan. Therefore, this study aims to detect the type and extent of relationship between these two factors.
Methods: A descriptive cross sectional study was conducted on a sample of 140, which were 4-8 years old children with speech sound disorders, without any di
Kaleem Ullah1, Zafar Niaz2, Sami Ullah Mumtaz3, Sajid Abaidullah4*
...he mean atrial flow reversal was 0.28±0.04. There were 14 (28%) patients having left ventricular diastolic dysfunction.
Conclusion: COPD patients had left ventricular diastolic dysfunction and it was related to increase in pulmonary artery pressure.
...

Abdul Basit1, Muhammad Zeeshan Majeed1,4*, Sohail Ahmed2, Gohar Ali2 and Muhammad Javaid

...ide formulations of spinosad. Refractive sheets of red, orange, yellow, green, blue, black and white were installed in M. charantia beds at three different angles (30º, 60º and 90º). Results revealed that control treatment exhibited significantly higher fruit fly infestation (60%) with minimum marketable fruit yield (374g/bed) than all other treatments. Among treatments, yellow colored refractive sheet treatments exhibited maximum fruit fly inci...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ection measures are necessary and we propose the continuous epidemiological surveillance to support the prevention of the viruses spread into non-epidemic areas.
...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...mune response is still unsatisfactory, especially in large animals. The present study aimed to investigate whether tissue plasminogen activator (tPA) could be applied as signal peptide for enhancing the effect of somatostatin DNA vaccine. A novel recombinant plasmid pEGS/2SS-tPA encoding two copies of somatostatin genes fused to tPA signal sequence was succesfully constructed and identified in MCF-7 cells. Subsequently, thirty female mice randoml...

Rizwana Qamar1, Maria Ghias1, Fida Hussain1, Sajida Habib1, Muhammad Khuram Razzaq2*, Muhammad Aslam1 and Imran Habib3 

...Agricultural Research Faisalabad for the screening of genotypes against drought. Ten hybrids along with their 13 parental inbred lines were grown in polythene bags following factorial structured completely randomized design by three replications against three levels of water stress. Data was recorded on root length (cm), shoot length (cm), plant fresh weight (g), plant dry weight (g), chlorophyll fluorescence, leaf temperature (°C) and relative water conte...
Ayesha Iftikhar1,*, Muhammad Asif Aziz1, Muhammad Naeem1, Munir Ahmad1 and Tariq Mukhtar2
...d predation rate is necessary to predict the population dynamics of this beetle against cotton mealy bug. Age-stage, two sex life tables of zigzag beetle were constructed at three different temperature regimes: 24 ± 0.5 °C and 27 ± 0.5 °C with 60-70% R.H. and ambient condition (32 ± 4 °C; 16-50% R.H.) with 14:10 h (L:D) photoperiod using cotton mealy bug as a host. According to the results, the immature duration and adult longe...

Muhammad Fiaz1*, Muhammad Afzal1 and Muhammad Zeeshan Majeed1,2 

...cy of spirotetramat and Isaria fumosorosea formulations was assessed in spring 2016 and 2017 against Asian citrus psyllid Diaphorina citri, one of the economic insect pests of citrus plants and a putative vector of Huanglongbing worldwide. Treatments were comprised of label-recommended (FD) and its half (HD) dose rates of spirotetramat and I. fumosorosea formulations. Both treatments were applied either alone or in binary combinations. There was a significant ...
Masood Nizam Tabassum1*, Muhammad Ather Khan2, Saira Afzal3, Amir Gilani4, Abdul Wahab Gureja5, ShafaqTabassum6
...nts. After simple random sampling 1120 patients coming from different parts of the Punjab province were included in this study. Data were collected and analyzed by SPSS version 22.
Results: A total of1120 patients were included in this study; the mean age of cases was 33 ± 16.76 years with minimum and maximum age of 15 and 100 years. The median and mode of ages was 27 and 15 years, respectively. The most common age group was 15-24 yea...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Haijun Cui1 and Xin Zhang2
...They were caged with the same batch of non-declawed hens. Compared with intact control, laying rate was significantly increased in groups 3 and 4, while percentage of defective eggs was visibly decreased in group 3. Hatching experiment showed a considerable depression in egg fertility and hatchability of setting eggs in group 4. Egg quality analysis demonstrated the significant increases in shell thickness among declawed treatments and yolk color in group 2. O...

Saeed Ullah1, Amanullah Jan1, Murad Ali2*, Wiqar Ahmad3, Hafeez Ur Rehman2, Muhammad Ishaq2 and Bilal Ahmad2  

...turity also remained the same (185 days) for the P2O5 levels and remained statistically similar with each other (185 days) compared to the control (188 days). Interaction of 70 kg P2O5 with 10 kg Zn ha-1 produced maximum HI (35%). This study suggested that 70 kg P2O5 ha-1 and 10 kg Zn ha-1 should be applied in bands for enhanced yield and related growth traits of the chickpea crop. Interaction among nutrients and application methods showed that application of ...

Wasim Javaid1, Farid Asif Shaheen1*, Farah Naz2 and Muhammad Usman Raja2 

...siana is recommended for safer storage of chickpea, pulses and grains. 

...

Tanzim Ullah Khan1*, Mohammad Tariq Jan1, Ahmad Khan1, Gulzar Ahmad2, Muhammad Ishaq2, Khilwat Afridi2, Murad Ali2, Muhammad Adeel Qureshi2, Imtiaz Ahmad3 and Muhammad Saeed2  

...grains spike-1 (42), thousand grains weight (48.38 g), biological yield (7244 kg ha-1), grain yield (2644 kg ha-1) and harvest index (36.30 %). More productive tillers m-2 (5.4%), leaf area (6.7%), grains spike-1 (5%), 1000 grain weight (4.4%), biological yield (5.8%), grain yield (16.7%) and harvest index (11.2%) were observed in PM incorporated plots compared to control. Optimum population of productive tillers (239), wider leaf area (27.9 cm2), more number ...

Imadud Din1*, Fazal Munsif1, Irfan Ahmad Shah3, Hamayoon Khan1, Fahad Ullah Khan1, Ibrarullah2, Shahab Uddin4 and Tauqir Islam1 

.... Among the genotypes Pirsabak-08 excelled for dwarf traits, while Hybrid-403 was found early maturing genotypes. Grain yield was positively correlated with productive tillers m-2, and biological yield. Genotypes PR-110 and Hybrid-404 resulted in superior grain yield among the genotypes. The results should help plant breeders to utilise the most promising wheat genotypes of this study in future breeding programmes for enhancing wheat cultivars.
 

Syed Muhammad Hassan Raza1*, Syed Amer Mahmood1, Veraldo Liesenberg2 and Syed Shehzad Hassan1  

...tacks on rice crop using satellite thermal datasets. The area under investigation was 13700 km2 area out of which 8627 km2 was covered by rice plantations. Thermal datasets covered the area of 8627 km2 comprising 10663 pixels which returned 10663 spatially distributed temperature values. Six, thermal datasets were obtained for the duration between (April 03-June 22) 2014, with a temporal window of 16 days to delineate YSB vulnerability from egg laying to pupa ...
Fouzia Hadi Ali1, Farhat Naz2, Aban Abid Qazi3
 
...f Punjab. The stratified sampling design was employed to select a sample from the population. Out of 80, a total of 53 hospitals were consented to participate in the study. A structured questionnaire was administered to a sample of 1100 doctors with a response rate of 83%. The sequential model was tested by applying Partial Least Squares Structural Equation Modelling 3.2.
Re...
Maaz Ahmad1*, Mussab Ahmad2 and Tehreem Munir3
...hrough systematic random sampling was conducted in Lahore urban community.After initial diagnostic investigations in 200 patients diagnosed as cases of FD fulfilling Rome III criteria were recruited after taking written consent and randomly assigned to one of 2 treatment groups (CP extract group or placebo) in equal number. A 7 days medication free period was observed before the start of trial. Each patient received the treatment for 6 weeks. The primary outco...
Farhat Ijaz1, Rana Khurram Aftab2*, Samia Jawed3
...formed using independent sample t-test. The P value ≤ 0.05 was considered statistically significant.
Results: Mean HOMA-IR and TNF-α values were significantly (p-value <0.01) higher in obese diabetics (17.13+8.77) and (10.96+4.69), respectively when compared to non-obese Type 2 diabetic patients (3.40+5.05) and (3.49+2.36) respectively. Mean HOMA-IR in males was 6.52+7.03 and in females was 12.85+10.54 (p-value 0.006). 
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
... of tuberculosis is necessary for timely control of the disease. GeneXpert test has already been implemented by World Health Organization to diagnose the infection on urgent basis.
Objectives: This study was designed to apply GeneXpert MTB/RIF assays for the detection of rifampicin resistant tuberculosis and validation of assays by comparing with conventional standard dr...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
... total of 12126 clinical samples were collected from patients presenting to Jinnah Hospital, Lahore. Every sample was processed for bacterial culture. Bacterial identification was performed according to standard guidelines. Every gram-negative isolate was further processed for antimicrobial susceptibility testing by modified Kirby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
...b>Results: Out of 66 samples, frequency of TEL-AML1 fusion oncogene was detected in 5 subjects (7.6%). Almost all TEL-AML1 positive patients carried FAB ALL-L1, B-Lineage immunophenotype, mean hemoglobin level of 6g/dl and age from 3 to 5 years. All these features are related to good prognosis.
Conclusion: To conclude, we reported 7.6% frequency of TEL-AML1 fusion gene, in our study subjects, which is different from that reported in west...
Muhammad Nusrullah*1, Muhammad Younus2 and Yasir Nasir3
...tion was obtained. Blood sample was obtained anaerobically from the radial artery and from a dorsal hand vein using 2 separate 5cc BD heparinized syringes with needle size 22G for each patientand pH, PCO2, and HCO3 were analyzed. All the collected data was entered and analyzed on SPSS version 21. 
Results: The mean age of the patients was 52.68±10.51 years. Strong relationship was found between...
Faisal Masood*1, Ranjit Kumar Sah2 and Ahmad Humayun Sarfaraz3
...n’s fracture by Faisal Technique.
Methods: A total of 10 cases of volar Barton’s fracture fulfilling our inclusion and exclusion criteria were included in our study from August 2015 to August 2016. These cases, presented at our department, were managed with closed reduction under image intensifier by dorsiflexing the wrist and reducing the fragment by ligamentotaxis and percutaneous pinning in anti-glide fashion from dor
Shumaila Irum1, Muhammad Zafar Iqbal*2 and Fatima Naumeri3
...usly published scores of same medical college, it also highlighted the areas needing further improvement.
...
Muhammad Younus1*, Sabah Usman2 and Samia Jawed3
...on-probability purposive sampling. Informed written consent was taken from all subjects. The demographic information of these subjects such as name, age, sex, height, weight, body mass index, waist circumference, and waist-to-hip ratio were recorded. Spirometry of all the subjects was performed on Spirolab iii. Correlation between FVC, FEV1, FEV1/ FVC ratio and body mass index, waist circumference, and waist to hip ratio was measured by Pearson coefficient. A ...
Mumtaz Akhtar, Iffat Naheed, Khadija Waheed* and Amna Khanum
...robability and purposive sampling techniques were used. A total of 220patients undergoing normal vaginal delivery at term after administration of per rectal misoprostol were enrolled with 95% confidence interval (C.I). The error margin was 5% and the prevalence of postpartum haemorrhage was 16.5%. Data was entered and analyzed through SPSS 17. In this study, the mean age of study participants was found to be 29.89±5.89 years while the mean gestational a...
Muhammad Anwar Iqbal1, Habib Rehman2, Ali Hussain3,*, Faiza Jabeen4, Ijaz Ahmad2, Kamran Ashraf5, Muhammad Irshad Arshad6 and Omaima Khan7
...he effect of mannan-oligosaccharides (MOS) as nutritional supplementation on zoo-technical parameters, visceral organ weights, serum mineral levels and caecal concentrations of E. coli and Clostridium spp. in Japanese quail (Coturnix coturnix japonica). Japanese quail (one-day old; n = 1320) were taken and randomly separated into four groups (n = 320), having 8 replicates (n = 40) in each group. Corn-based poultry feed (ba
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
...ng. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–15.7), 6.90% (95% CI 2.6–14.4), 5.49%, (95% CI 1.8-12.4) and 4.35% (95% CI 0.9-12.2) from Wenzhou, Lishui, Jinhua and Ningbo counties, respectively. For the first time, we investigated the seroprev...
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...e was subjected to blood sampling (n=20 each). DNA was extracted, purified, quantified and amplified through PCR by using specific primer set. Then Sanger’s method of DNA sequencing was used. Multiple sequence alignment demonstrated that sheep and goat were having the wild base Guanine against which the cattle and buffalo depicted the mutant form Adenine. Results of this study illustrated the genetic tendency of large domestic bovines towards aggression ...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
Ansar Ahmed Abbasi1,*, Kathrin Blasius2-4, Imtiaz Ahmed6, Hao Hu7, Sylvie Picker-Minh2-4,8, Muhammad Nasim Khan5, Khalid Hameed1, Aneela Gulnaz1, Zahid Latif5, Abdul Rauf5 and Angela M. Kaindl2-4,8
...rtex, and intellectual disability. To date 17 genes have been linked with this disease. Biallelic mutations in the abnormal spindle-like, microcephaly associated gene ASPM causes MCPH type 5, the most common MCPH subtype. In the current study we recruited two families from Sialkot region of Pakistan which were assessed by whole exome sequencing and cosegregation analysis. We identified a previously described mutation c.4802C>G (p.S1601*) in the AS...
Hanif Ur Rahman1, Umer Saddique1, Zahoor-ul-Hassan1, Shakoor Ahmad1, Muhammad Kamal Shah1, Said Sajjad Ali Shah1, Farhan Anwar Khan1Fazli Rabbani2, Muhammad Asif Hussain2, Attaur-Rahman2, Irshad Ahmad3,4 and Sadeeq ur Rahman2,*
...ailable regarding the causative agents of CCPP in sheep and goats in Northern Pakistan. The current study was thus aimed to identify relative abundance of members of MM in small ruminants suspected of CCPP in Northern region of Pakistan. A total of 300 samples, (150 sheep and 150 goats) were randomly collected from nasal discharge, pleural fluid and lung tissues, and streaked into Pleuropn...
Sana Rafique1, Hina Saqib1, Khushi Muhammad2, Nazia Akbar2, Abdul Rauf3, Muzafar Shah4,*
...ted with the convenience sampling which were admitted in Al-Khidmat foundation and King Abdullah hospitals in Mansehra, Pakistan, where patients were screened for dengue virus non-structural protein using Rapid Diagnostic kit for Dengue virus (DENV) i.e. SD BIOLINE Dengue IgG/IgM. Data was obtained from patient’s record, filled forms and through questionnaire. Total 1332 blood samples were collected from 3 tehsi...

Jahangir khan* and Abbas Ullah Jan 

.... For data collection, a sample of 200 households was randomly selected from urban and rural region of Peshawar district of Khyber Pakhtunkhwa Province. Sampled households were interviewed face to face. An open-ended CVM question was designed to elicit households’ willingness to pay (WTP) and a linear regression analysis conducted to identify important determinants of households’ WTP. Results show that 93% of respondents are willing to pay a price ...
Rafa Almeer1,*, A. Alqarni1,2, S. Alqattan3, S. Abdi4,*, S. Alarifi1, Z.Hassan5 and A. Semlali6
...ty was examined by MTT assay and gene expression of three tissue inhibitors of metalloproteinase (TIMPs) and two matrix metalloproteinase (MMPs) were measured by real-time PCR. All subgroups exhibited altered morphology with accelerated detachment compared to untreated cells. The MTT assay after 48 h revealed that treatment with H1 and H2, reduced cell viability by 48% and 91% respectively, compared to that of untreated cell...

Abdur Rehman and Zahir Shah 

...ent uptake of pea [Pisum sativum L.] under silt-loam alkaline soil conditions. Treatments were arranged in randomized complete block design (RCBD) with three replications. The required Fe and Mo as [(NH4)6Mo7O244H2O] and FeSO4.7H2O respectively were applied to soil followed by thorough mixing before sowing. Both Fe and Mo induced significant increases in fresh pods pea yield and nutrient uptake. Their combined effect was more prominent than their alone applica...

Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla

...ed from suspected animal samples (n=18) and were screened by real time-PCR. Additionally, to assess the phylogenomics, the N gene was amplified and sequenced from sheep and goats (n=12). Nucleotide identity among Hail strains was identified to be 96.2-100%, while identity with previously sequenced Saudi Arabian strains and with reference PPRV retrieved from GenBank was found to be 94.1-98% and 81.2-100%, respectively. Phylogenetic analysis revealed that Hail P...

G. Venkatesan* and Amit Kumar  

... including India. The causative agent, Orf virus (ORFV) belong to the genus Parapoxvirus (PPV) of the family poxviridae produces a typical papillomatous, cauliflower like external growth around mouth regions and rarely the lesions are found in other parts of the body and internal organs. Another characteristic feature of ORFV including other PPVs is the ability to re-infect the same host and conferring short term immunity in...
Mudassar Javed1,Muhammad Zeeshan Majeed1,*, Muhammad Sufyan1, Sajjad Ali2 and Muhammad Afzal1
...okra variety ‘Pusa Sawani’ under field conditions during 2015 and 2016 in a randomized complete block design. The average number of lepidopterous larvae in plots treated with the synthetic insecticides was 1.87 and 2.12 larvae/plant in 2015 and 2016, respectively, compared with 6.5 larvae/plant in untreated control plots. For the botanicals, an average of 2.57 and 2.92 larvae/plant was recorded in 2015 and 2016 respectively, compared with 4....
Kamran Khan1, Sarzamin Khan2, Nazir Ahmad Khan2,* and Naseer Ahmad2
...n standard concentrate basal diet, whereas in the treated groups, the basal diet was supplemented with low (3.5%; LCF) or high (7%; HCF) level of crushed flaxseed and low (1.5%; LFO) or high (3%; HFO) level of flaxseed oil until slaughtering (day 90). Carcass fat deposition was greater (P < 0.05) in the supplemented groups as compared to the control group. The contents of myristic acid and palmitic acid reduced (P ˂ ...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...e collected 86 different samples (feces 64, hair 13, blood 5, tissues 4) from 5 geographic langur populations of Pakistan and Azad Jammu and Kashmir (AJK) and succeeded in extraction of DNA from 23 samples, which were used for further genetic analysis. RAPD makers (n=8) produced 245 bands (30.62±2.87 Mean±SE / primer) of different molecular weights (126-3342 bp), of which, 96 were population specific. Polymorph...

Muhammad Khalid1,2 and Muhammad Naeem2* 

...ximate composition of 72 samples. On wet weight basis, mean percentage for water was 80.76 %, ash 3.40 %, 4.31 % for fat and 11.53 % for protein in C. idella. Percent water contents showed inverse relationship with % protein (dry and wet body weight), % ash and % organic contents (wet body weight). Wet body weight (W) showed negative correlation (P < 0.001) with percent water, percent fat (dry and wet body weight) and positive correlation with percent prote...
Md. Abu Affan1,*, Abdulmohsin Al-Sofyani1, Adnan Jaman Turki2 and Mohammed S.B. Hariri3
...xygen (DO), temperature, salinity, pH, ammonia, turbidity, pesticides, toxins, and diseases. The study was carried out to find out the reasons of sudden fish killing. Therefore, physico-chemical and biological parameters, and postmortem, especially the gills and gut content of dead fishes were studied. The dead Tilapia tilapia (Tilapia), Chanos chano (Milkfish) and Parapriacanthus ransonneti (Glassfish) were found with wide mouth opened, s...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...luding 19,224; 19,235; Pusa Swami; Ikra-1; Ikra-2; and Sabzperi China Red, were selected from our previous study according to their galling index and level of susceptibility. These genotypes were used as test genotypes for the interaction between M. incognita and the entomopathogenic fungus, Lecanicillium muscarium. The initial inoculum was measured before okra was seeded in the field. At harvest, the numbers of root galls, egg masses, and...
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
...d within species and the sample from Kaidu River in South Xinjiang might be a cryptic species or subspecies of G. dybowskii. The phylogenetic analyses from 12 concatenated H-strand-encoding protein genes were conducted by Neighbor-Joining method to reveal the evolutionary relationships within subfamily Schizothoracinae. Three different grades of schizothoracine fishes were well recognized from each other in branching diagram. The primitive group and the...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...ion, but also the codon usage of genes. Whereas a significant variation among Coilia species was detected in length of the control region, mainly caused by the variable number of tandem repeats. Phylogenetic analysis was performed based on 13 concatenated mitochondrial protein-coding genes from 8 Coilia mitochondrial genomes. The results supported that C. lindmani at first clustered with C. nasus and C. grayii which had close...

Dilshad Ahmad1*, Muhammad Irfan Chani1 and Muhammad Afzal2 

...aucratic influence and misallocation of credit utilization are major constraints in lowering overall agriculture output. Adequate farmer’s friendly policies and proper legislative measures are necessary for the smooth flow of increasing agriculture output and overcoming financial constraints. 

...

Fazal Munsif1, Muhammad Zahid1, Muhammad Arif2, Kawsar Ali3 and Ijaz Ahmad

...ay planting can be compensated by increasing the cropping intensity on same field. A field experiment was carried out during the year 2015-16 at Palatoo Research Farm of Amir Muhammad Khan Campus Mardan, The University of Agriculture Peshawar. The experiment was consisted of traditional cultivation of sugarcane both in winter season (20th September, 10th October, 30th October and 20th November) and spring season (20th Februa...

Mohammed Zaki Fathy* 

...ure were recorded. Blood samples were collected at 0, 20, 40, 80 minutes and 12 hours after administration for detection the hematological changes. Results showed that the onset, duration and total recovery times of anesthesia were 7.33±3.05 min and 3.33±1.53 min, 26.33±3.51 min and 45±3.00 min and 64±4.58 min and 86.67±6.11 min in G1 and G2 respectively. In G1, presence of a significant decrease in RBCs, PCV and Hb...

Raphael Olanrewaju Babatunde, Adeyemi Esther Omoniwa and Miriam Ukemenam 

... Kwara state, Nigeria. A sampling technique comprising of three stages was used to randomly select 200 farming households in the study area. Data were collected using questionnaire and were analyzed using the Gini-coefficient and the Ordinary Least Square method. The result showed an overall gini-coefficient for gender inequality in schooling of 0.35, with the value for boys and girls at 0.35 and 0.49 respectively. Mother’s education, age of the househol...

Shahen Shah1*, Manzoor Hussain1, Arshad Jalal1, Mohammad Sayyar Khan2, Tariq Shah1, Muhammad Ilyas1 and Muhammad Uzair3 

...ductive tillers m-2, thousand grain weight, grains spike-1, biomass yield and value cost ratio (VCR) of wheat were significantly affected by N and S. Sulfur and its application time prominently influenced the productive tillers m-2 and biomass yield. Nitrogen at 150 kg ha-1 resulted in more number of productive tillers m-2, less un-productive tillers m-2 and higher thousand grain weight, biological yield and higher value cos...

Irfan Ali Sabir1, Saeed Ahmad1*, Muhammad Nafees2, Ahmad Sattar Khan1, Maryam3 and Ishtiaq Ahmad

...9 B°) in ‘Chaunsa’, while, titratable acidity (TA) was lowest in ‘Lambay Alfansu’ and ‘Momi-K’. However, reducing sugars (RS) (4.83%), non-reducing sugars (NRS) (16.38%) and total sugars (TS) (21.21%) were significantly high in ‘Surkha Burma’. Ascorbic acid contents (AA) were high in ‘Momi-K’, whereas, total antioxidants (0.68 mM Trolox g-1), total carotenoids (TC) (52.0 µg 100-1) and flavon...

Muhammad Siddiq1*, Sahib Alam2, Hamid Ullah Shah2, Zafar Iqbal2, Kalsoom Siddiq3 and Taufeq Ahmad4 

...h consecutive use of the same oils for up to three sessions. However, it is recommended that further studies on the repeated frying practice on the same oil sample beyond three sessions should be undertaken to draw more conclusive results 

...

Ansaar Ahmed1, Imtiaz Hussain2*, Ibni Amin Khalil3, Subhanulla3, Gulzar Ahmed3, Imtiaz Ahmad3 and Muhammad Imtiaz2  

... 16 and 22 percent water saving with bed planting in comparison with other practice in 2014-15 and 2015-16 cropping cycle, respectively. The study showed that farmer practice of manual planting of maize and broadcasting of wheat can be replaced with bed planting of maize and zero till drill planting of wheat, respectively to save water, labor and improve maize-wheat system productivity and water use efficiency. 

...

Sadiqa Begum1, Murad Khan2* and Noor Pao Khan

... proportionately from 93 sample respondents. Budget allocation technique is used for calculation of cost, revenue and profit and dummy approach is used for comparative analysis while multiple regression model is used to estimate the relationship between inputs and output of wheat. Per acre yield of wheat production in district Mardan was 1809.032 kgs. while in district Peshawar it was 2143.230 kgs. Total cost of wheat per acre in district Peshawar was more tha...

Muhammad Haroon Hullio1,4, Abdul Ghani Lanjar2, Abdul Rahman Dhuyo3, Imran Khattri4 and Agha Mushtaque Ahmed4* 

...The study was based on a sample of 25 tillers covering all area from an acre in order to record the pest population randomly. Later, the pest population was also recorded at particular stages of rice (tillering, milking and booting) with effect of abiotic factors (temperature and relative humidity). In last, the varietal screening of 30 local rice varieties in term of pest population was observed. The data were collected for two rice growing seasons 2012 to 20...
Hülya Şereflişan and Beyza Ersoy Altun*
...he situation was vise versa for fatty acid compositions. The proportions of Omega-3 (n3) were higher than those of Omega-6 (n6) in both of the mussels. n6/n3 ratio, which was 0.90 for A. pseudodopsis and 0.99 for U. tigridis, is an index for comparing the relative nutritional value of seafood oil.Leucine and lysine were the most important proportions of the essential amino acids, and tryptophane was the minor one. The ...
Azmat Hayat Khan Khattak1, Abdul Wasay2, Tariq Ali1,*, Muhammad Iqbal1, Khisrao Kalim1, Mohammad Farooque Hassan3, Muhammad Mobashar2, Nazir Ahmad2, Abid Iqbal2 and Muhammad Nauman ul Islam1
...and C were also fed with same feeding regimen with the corresponding age adjustments. The results showed that mean feed intake in weaning age groups-A, B and C was 5.71, 4.34 and 6.02 kg, respectively. Mean weight gain was 5.56, 5.02 and 6.70 (kg/15 days), respectively. Whereas, mean feed efficiency was 0.07, 0.13 and 0.09, respectively. Significant (P > 0.05) interaction was recorded for feed intake and feed efficiency between weaning age and feedin...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...n Pseudomonas aeruginosa is a major barrier to successful treatment of infection. Additional and novel measures to control this pathogen are needed, along with contemporary information about antibiotic resistances that are present in isolates from different environments. In the present study 72 samples from blood, 43 from sputum, and 19 were obtained from tracheal aspirates patients suffering from chronic and acute lu...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3,*
..., arrangement and codon usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii, O. rebecca and Odontamblyopus sp. were all circular double-strand molecules, 17245 bp, 17009 bp and 17004 bp long, respectively. Compared with other bony fishes, the three species shared the similar featu...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5
...d 42.57% was observed at same concentration after exposure period of 72 h. Least repellency (11.23%) was recorded at 5% concentration of n-hexane based extract of J. curcus after exposure period of 72 h. Repellency was found significantly influenced by concentrations of plant extracts and the exposure time. Overall results showed that methanolic extracts were more effective than other three solvents and extract of C. paradise was found com...
Hafiz Manzoor Ahmad1,*, Hamad Bin Rashid1, Muhammad Arif Khan1, Asim Khalid Mahmood1, Muhammad Ijaz1, Shahid Hussain Farooqi1, Muhammad Rafi Ullah2 and Usman Ghani1
...g has failed to induce a safe and deep anesthesia for surgical as well as painful conditions. However, this problem can be solved with usage of anesthetic combinations. This study was aimed at comparative evaluation of two anesthetics combinations viz. ketamine-xyalzine (K+X) and ketamine-xylazine-diazepam (K+X+D). A total of 20 ducks presented at pet center University of Veterinary and Animal Sciences Lahore were div...

Jam Ghulam Mustafa Sahito1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1 and Inayatullah Rajper2  

...tula and Scirtothrips dorsalis was recorded on weekly basis cotton grown with both green manure and IPM treatments. The green manure treatments suffered comparatively higher population of B. tabaci (0.83±0.09) and S. dorsalis (3.43±0.47) than IPM treatment with population of 0.46±0.05 whiteflies and 1.98±0.72 thrips, respectively. However, IPM cotton suffered higher A. biguttula biguttula populati...
Nevran Eylem Akman Gündüz1,* and Özgür Özcan2
...h a diet incorporation assay. Different concentrations (2, 5, 10, 50, 100, 200, 500 and 1000 mg/L) of GA3 were added to the synthetic diet and protein, lipid, carbohydrate and glycogen levels in the hemolymph were evaluated for the GA3 concentrations. The hemolymph protein level of the larvae increased significantly at 5, 10, 50 and 200 mg/L with respect to the control group. The lipid level of hemolymph fluctuated among the tested GA
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
... dilutions of Nigella sativa (N. sativa)alcoholic extracts against the PPRV was investigated in vitro. Vero cell lines infected with PPRV were treated with six dilutions alcoholic extracts of N. sativa (200, 100, 50, 25, 12.5 and 6.25 µg/mL). The antiviral effects were judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric (MTT) as...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...c approaches and develop safe biological/microbial insecticides from NPV isolates, which in future may effectively control S. litura.
...
Xiaqing Chen, Yü Huang and Zhen Huang*
...zim offered a promising, safe and effective alternative to fungicides in treatment against S. shiraiana, popcorn disease of mulberry.
...

Ghulam Abbas1, Khalid Pervez Akhtar1, Muhammad Ahsan2, Muhammad Jawad Asghar1, Fiaz Ahmad1 and Muhammad Rizwan3* 

...lign: justify;">Yellow mosaic disease (YMD) caused by mungbean yellow mosaic India virus (MYMIV) is an important constraint of mungbean during the summer season in Pakistan. The use of resistant varieties is the only way to reduce the losses caused by YMD. Resistant sources had already been reported in mungbean, but no such information seems to be available for mung × mash interspecific recombinants. In this study, 72 ...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
...sm. In vitro bioassays were used to evaluate the efficacy of T. hamatum and difenoconazole alone or its combinations against S. sclerotiorum. The results showed that T. hamatum could infect and destroy the mycelia and sclerotia of S. sclerotiorum by mycoparasitism. The cell-free culture supernatant and ethylacetate extracts from T. hamatum inhibited mycelial growth of S. sclerotiorum, with EC50 value ...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...d by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycosides. No resistance was observed against vancomycin and linezolid. Among genes involved in host immune evasion, Staphylococcus protein A (spa) was identified most frequently (81%) and proportions of capsular polysaccharides (CPs8), clumping factor A (clf A) andintracellular adhesion A (ica A) were 78%, 68.5% and 40%, r...

Ali Sher1*, Khalid Naveed1, Gulzar Ahmad2, Ayub Khan1, Muhammad Saeed1 and Shah Masaud1 

...arch Institute (CCRI) Pirsabak, Noweshera, Khyber Pakhtunkhwa, Pakistan during 2014-15 and 2015-16 to study the response of wheat to N, Zn and Fe application. Treatments included three levels of N (90, 120 and 150 kg ha-1), three concentrations of Zn and Fe (i.e. 1, 2, 3 kg ha-1) each along with two controls i.e. no micro nutrients no water (control-I) and no micro nutrients + water (control-II). The N was soil applied and MN as foliar spray at booting stage. ...
Gokhan Sahin1, Ecevit Eyduran2,*, Mete Turkoglu3 and Fatma Sahin2
...s the ratio of RSS to n (sample size) for penalty= -1. The smallest GCV values were set at number of terms (4). Goodness of fit criteria exhibited that the MARS prediction model had a very good fit for a cross validation of 3. As a result, the obtained results may be baseline information about global irradiation parameters at location of migratory birds in Iğdir, Turkey for next studies with the scope of global warming.
...
Shoukat Ara1, Sheikh Adil2,*and Manzoor Ahmad Khan3
...b>) group were fed the basal diet, where as other groups the groundnut cake was replaced with 5 (T2), 10 (T3), 15 (T4) and 20% (T5) Azolla. The results revealed that inclusion of Azolla showed a non-significant increase (p>0.05) on the body weight gain of grower birds. Further, the SGPT and SGOT levels of birds did not differ among various treatments at 19 weeks of age. A non-significant (p>0.05) ...

Asinamai Athliamai Bitrus1*, Olabode Mayowa Peter2, Muhammad Adamu Abbas3 and Mohammed Dauda Goni

...aureus being a highly versatile pathogen can quickly acquire resistance genes. The development of resistance in bacteria predates the era of antibiotic use. However, resistance developments in S. aureus have been reported since the early 1940-ties, when penicillin resistant S. aureus was first reported. Ever since, this pathogen has gain global notoriety as the most common cause of nosocomial, community and livestock associated infection. The mechanism of resi...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Mujahid Iqbal3  

... crop by mobile phone. A sample size of 60 farmers was selected by systematic sampling technique using the available list of the farmers provided by Agricultural Extension Department. The respondents were than randomly assigned into two groups, one group was comprised of experimental group (to whom a set of advisory instructions were sent by mobile phone) and other was control (face-to-face). Two- population independent t-te...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...(n=32), P.aeruginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clinical samples of hospitalized patients. Out of these 100 carbapenem resistant isolates, 93 and 89 isolates were positive for cabapenemase and β-lactamase production, respectively. Notably, 3 (3.3%) of MBL producing strains harb...

Aftab Khan, Shahid Ali, Syed Attaullah Shah and Muhammad Fayaz 

...ected through multistage sampling technique. Primary data on net revenue from maize crop was collected through an interview schedule and secondary data on temperature and precipitation was collected from Regional Metrological Department, Peshawar. Cross sectional Ricardian Model was used to estimate the effect of temperature and precipitation on net revenue. Results of the study revealed that temperature has positive effect and temperature square has negative ...

Muhammad Faheem Jan1*, Asad Ali Khan1, Waqas Liaqat1, Haseeb Ahmad1, Muhammad Dawood Ahmadzai1 and Wazir Rehan

...anic fertilizers is necessary for sustainable crop productivity. Field trial was performed at Agronomy Research Farm of The University of Agriculture Peshawar during summer 2016. Two factors were studied i.e. hybrids (DK-Garanon, Pioneer-3025, WS-666, and Pioneer-3164) and potassium ratios (Organic vs inorganic). The organic source includes poultry manure (PM) while inorganic source includes sulphate of potash (SOP). Potassium (K) was applied to field in such ...

Iqbal Javed1*, Amar Razzaq2, Mudassar Yasin3, Muhammad Ali Imran4, Haroon Javaid5, Iftikhar Nabi6, Anum Sardar1 and Shahbaz Ahmad

Dilara A. Bukhari1,*, Naureen Fatima1 and Abdul Rehman2

 

...leaf litter, garden soil samples, 15% were from animal waste and 10 % were from moist soil of the crop area. A 650bp of cry11 gene was amplified by PCR and seven isolateswere found positive for cry11 gene. The 16S rDNA study exposed that these screened B.t. confirmed 99% homology with B.t. serovar tolworthi, B.t. str. Al Hakam, B.t. serover thuringiensis, B.t. serovar konkukian, B....

Nusrat Habib1*, Asim Zubair2 and Aisha Siddiqua3 

...tricts through purposive sampling for 100 farms in different locations of selected districts. The objective of the study was to estimate determinants of sunflower area and yield in the study area. To determine the role of different factors that were affecting the area and yield of sunflower in Khyber Pakhtunkhwa, multiple regression analysis was applied. The model pointed out that the area under sunflower have opposite relation with the seed cost, land rent, a...

Safina Naz1, Muhammad Akbar Anjum1, Saeed Akhtar2, Syed Atif Hasan Naqvi3* and Muhammad Asif Zulfiqar

...ich were higher than the safe limits recommended by WHO (1996). All the vegetables grown with tubewell water had significantly lower heavy metal contents, well below the critical limits. The concentration of Pb in tomato and cauliflower, Cd in tomato and spinach, and Fe in spinach irrigated with canal water were also detected beyond the safe limits. The study concludes that the continuous use of sewage water deteriorates or ...

Muhammad Javed Iqbal and Muhammad Naeem* 

... of 15 aquaria having 20 samples each were arranged in triplicate for 90 days to study the effect of five feeding groups on Length-weight relation and external morphometric variants of Labeo rohita. A total of 75 fish samples @ 5 fish samples per aquaria were analyzed in present study. T1 showed the highest values for mean wet weight 11.32±1.78, Total length 10.54±0.51, Fork ...

Safina Naz1, Khushbo Hussain1, Muhammad Asif Zulfiqar2* and Syed Atif Hasan Naqvi3
 

...halene Acetic Acid & salicylic acid and orange oil extract on physical and chemical parameters gave significant response. NAA promotes vine length upto 12.66 feet while chlorophyll content was 72.50 SPAD Value, while fresh weight of leaves is 5.1g, dry weight is 0.41g, while fresh weight of fruit is 121.66 g and fruit length is 14.11 cm. Salicylic acid promoted vine length upto 11.33 feet while chlorophyll contents were calculated as 71.09 (SPAD Value), wh...

Mian Noor Hussain Asghar Ali, Liaquat Ali Jamali, Shakeel Ahmed Soomro*, Shakeel Hussain Chattha, Khalil Ahmed Ibupoto, Naseer Ahmed Abbasi and Noor Mehdi Qumi  

...ith sugarcane trash. The samples of sugarcane were taken at an interval of 24 hours to determine different quality parameters i.e. cane weight, brix, pol/sucrose, purity, fiber and commercial cane sugar. The results revealed that weight loss and fibre content throughout increased with increasing storage period. However, a decrease was observed with increasing storage period for brix content, sucrose content, purity percentage and commercial cane sugar percenta...
Zain ul Abidin1, Aisha Khatoon2, Abdul Whab Manzoor1,*, Nida Arooj1, Sajjad Ali1 and Muhammad Numan1
...div>...
Haroon Rashid1,*, Khushi Muhammad1, Masood Rabbani1, Muhammad Sarwar Khan1, Tasleem Ghori2, Mamoona Chaudhry3, Hamad Bin Rashid2, Muhammad Zubair Shabbir1, Muhammad Hamid Chaudhary4, Muhammad Asad Ali1, Mian Muhammad Khubaib Sattar5, Javed Muhammad1 and Bhushan Jayarao6
...ance. Using a grid-based sampling strategy, soil samples (n=1985) were collected from villages (n = 397, 5 samples/village) and examined for Bacillus anthracis, using real time PCR assays. Soil samples were found positive for plasmid DNA of Bacillus anthracis (CapB) in 3 villages (n= 3/1985, 0.15%), where...
Imran Taj1,*, Ferhat Abbas1, Zafar Ahmad1, Mohammad Kamran Taj1, Deedar Ahmad2, Zahoor Ahmed2, Ghulam Mohammad2, Ajaz-ul-Haq1, Farooq Shahzad1 and Sabiha Azam3
...ts. A total of 204 fecal samples were collected from sheep and goats suffering from seasonal diarrhea. C. perfringens type D was identified through different biochemical tests and PCR. Antibiograms and lab animal trials were also carried out on local isolates of C. perfringens type D. Out of 204 analyzed samples, 89.18 % were found positive while 10.82 % were negative for C. perfringens type D. The divis...
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
...tnesses in bacteriophage samples stored at 4oC for up to one year. The results of this research indicated, that a standard refrigeration is suitable and economical means of long term storage with no fall of phage activity.
...

Tariq Aziz1, Iftikhar Hussain Khalil1, Quaid Hussain1,3*, Tariq Shah2,3, Nazir Ahmad1 and Amir Sohail1 

...cultivated cultivars (Pirsabak-08 and Janbaz) at University of Agriculture, Peshawar during 2013-14. All wheat genotypes (8 parents and 12 F3 populations) showed significant differences for all traits. Cultivar Janbaz had maximum spike length (13.9 cm) and maximum number of grains spike-1 (89.2). Parental line B-IV(N)5 had maximum single spike weight (4.6 g), 100-grain weight (4.6 g) and grain yield (32.0 g plant-1). F3 population B-RF7 × Janbaz had 100-...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
...ely for four days. Blood samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in serum biochemistry and histology. Serum glucose and cholesterol were increased while total protein was decreased. Histopathological result reveled that gills of experimental fish was damage severely resulting Necrosis, Damaged nuclei , ru...

Muhammad Abu Sufyan Ali, Syed Attaullah Shah*, Ghaffar Ali and Muhammad Fayaz 

...akistan), using land transaction records for 2015-16. Results from Hedonic Pricing analysis show that location, environmental and agricultural characteristics of a farmland determine its price. Residential and commercial encroachment on fertile irrigated farmlands at urban fringe is alarming. More than half of the sampled transacted farmland parcels were used for residential and commercial...
Rahim Dad Brohi1,*, Amir Bukhsh Kalhoro1, Allah Bux Kachiwal1, Illahi Bux Kalhoro1, Dildar Hussain Kalhoro1, Sarfaraz Ahmed2, Farhan Anwar Khan3, Hira Sajjad Talpur1, Zia-ur-Rehman1 and Dinesh Bhattaria4
...c regimens. The means of safe induction of anesthesia (propofol, 14.00 ± 0.2582; and thiopentone sodium, 14.83 ± 0.1667 seconds) and duration of anesthesia (thiopentone sodium, 14.50 ± 0.2236 and propofol, 13.22 ±0.1014 min), and a full recovery from anaesthesia (thiopentone sodium, 13.67 ± 0.4216; and propofol, 12.45 ± 0.2432 min) were differed from one another. Comparing the results of this study, it is concluded tha...

Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...usmn;0.71UmL-1). After desalted the CAT activity in gills of both control and insecticides exposed fish was calculated as 73.45±0.71 and 42.77±1.36UmL-1, respectively. The highest specific activity was observed in crude CAT extract from gills of control fish (112.53±0.28 Umg-1) as compared to exposed fish (82.69±1.12Umg-1). After desalted specific activity of gills CAT was lower in exposed fish (1...

Abdur Rehman1* and Shad Khan Khalil2 

...water), three chemicals (salicylic acid 0.5mM, potassium nitrate KNO3 1% and methanol 20% v/v basis) applied at three different stages (bud formation, 20% flowering and 20% pod formation). The experiment was laid out in RCB design with split plot arrangement having three replications. Moisture levels were allotted to main plot while treatments (application of chemicals at different growth stages and water spray) to sub plot. Chemicals were sprayed in solution ...

Shamsher Ali1, Zakir Ullah Jan1*, Zahir Shah2, Muhammad Jamal Khan2, Farmanullah Khan2, Inayat ur Rahman3 and Shah Fahad3 

...ety (Azam cv.) under the same application of fertilizer treatment and dozes during summer 2015. The field experiment was laid out in randomized complete block design with split plot arrangements having two maize varieties in main pot and six levels of fertilizer treatments in sub pots repeated four times. Fertilizers treatments were made from the application of Nitrogen (N), Phosphorus (P2O5) and farmyard manure (FYM) which was collected from cattle barnyard. ...

Ejaz Ashraf1*, Bushra Hassan2, Nadeem Anwar2, Hafiz Khurram Shurjeel3 and Shafique-ur-Rehman

...ators of development and satisfying the needs of fragile groups of rural communities. This study examined socio-economic factors of rural life which contribute in economic prosperity. A list of union councils and villages in tehsil Sargodha was obtained from local district office of the Population Welfare Department. All rural families live in tehsil Sargodha served as population in the study. Tehsil Sargodha was selected purposively. Of the 62 rural union cou...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...olism with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seeding on scaffolds to form systems as: keratinocytes monoculture (KM), fibroblasts monoculture (FM), co-culture of keratinocytes and fibroblasts (KM&FM). Systems was treated with 3.4 kPa pressure for 1 day, systems without pressure was considered as control groups. The mRNA expression and protein secretion of supernatant were teste...
Li Lv1, Liuan Li1,*, Ruibo Zhang1, Zhichao Deng1, Tianming Jin1 and Gaimei Du2
...ental groups and fed a basal diet supplemented with selenium enriched yeast at differing levels: 0.0 mg/kg (control group), 0.3 mg/kg (low-dose group), 0.6 mg/kg (medium-dose group) and 1.2 mg/kg (high-dose group). Each experimental group had four cohorts and each cohort had 10 hens. Experimental diets were fed for 28 d and daily egg production was recorded. Eggs were collected every seven days for determination of egg white, egg yolk and total egg selenium us...

Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil

...aneum) collected from Faisalabad (Fsd-strain), Jhang (Jh-strain), Sahiwal (Swl-strain) and Sargodha (Sgd-strain) districts of Pakistan. Bioassays with two frequently used insecticides, i.e. deltamethrin and permethrin and one biological insecticide, spinosad, were performed on these strains of T. castaneum. Toxicological studies demonstrated that Fsd-, Jh- and Swl- strains exhibited higher...
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
...leukin-6 (IL-6), lipopolysaccharide (LPS) as well as colonic mucosal tissue TLR4/9 and NF-kB. We got the following results: (1) The rat IBS-D model was successfully prepared; (2) Wumei Pill could reduce the contents of serum TNF-α, IL-6 and LPS in rats(P<0.05), and alleviate the inflammatory reaction and accumulation of bacterial endotoxins; (3) Wumei Pill could reduce the transcriptional level of TLR4/9mRNA, to dec...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...ells, whereas the granulosa cells were regressed and disappeared. Erythrocytes and leukocytes were also detected at the advance stage of atresia. Apoptosis in the granulosa cells were clearly detected. The chromatin condensation against the nuclear envelope, cell shrinkage, surface blebbing and generation of apoptotic bodies by cell fragmentation were ev...
Peng Xu1, Bin Liu1,2, Yongqiang Zhao3, Shicheng Lv3 and Changhu Lu1,*
...climate variables in the same year with GAMs indicated significant correlations between population size and mean maximum air temperature in November, mean minimum air temperature in December, mean air temperature in December, respectively. Time-lag effects were found between population fluctuation of red-crowned crane and the climate variables within 2, 4, 6 and 7 years earlier in December and February of the following year. Combination of the highest interpre...

Amar Razzaq1, Abdur Rehman4*, Abdul Hassan Qureshi2, Iqbal Javed3, Raheel Saqib5 and Muhammad Nadeem Iqbal6 

...erefore, one half of the sample consisted of modern and conventional farmers growing wheat crop and the other half of the sample consisted of modern and conventional farmers growing mango orchards. Economic analysis measures of benefit-cost ratio (BCR) and net present value (NPV) were estimated. The results of the study showed that the users of high-efficiency irrigation systems (sprinkler and drip irrigation) earned higher ...

Sajad Ali1*, Syed Asghar Ali Shah2, Jaffar Ali3, Muhammad Nadeem Iqbal4, Arshadullah Jadoon5 and Farmanullah

... mention that the public sale arrangement was not totally available in the local markets. Furthermore, the 68 percent in district Mansehra while, in the Haripur 46 percent farmers traded the pea’s output in the local markets. The left over peas output in both the selected districts were offered for sale in the regional markets that is Rawalpindi and Peshawar markets. The 82 percent of the peas producers availed the pri...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...chromatography. Enzyme assays of CueO protein with phenolic substrates revealed its laccase activity. The kinetic studies showed Km value of 0.2µM, kKcat 0.68 S-1 and Kcat/km 1.2S-1 µM-1 for 2,6-Dimethoxyphenol (DMP) and Km value of 0.25mM, Kcat 300 S-1 and Kcat/Km=1200S-1mM-1 for Syringaldazine (SGZ). Regulation of cueO in response to various concentrations of copper was stud...
Ammara Afzal1, Tazmeen Fatima1, Maham Tabassum1, Muhammad Nadeem2*, Muhammad Irfan3, Quratulain Syed2
...n (SHF) and simultaneous saccharification and fermentation (SSF) process. Fungal strains of Saccharomyces cervisae, Trichoderma viride, Trichoderma koninji and Trichoderma harzianum was used for subsequent process using saw dust as substrate. Three major processes convert lignocelluloses to bioethanol i.e. pretreatment of biomass, enzymatic hydrolysis of raw material a...

Fan Shuli1*, Ameer Hussain Jarwar1,3, Xiaoyan Wang2, Long Wang1 and Qifeng Ma

... an estimate per capita usage of edible oil in the country is 15 kg, this is even higher than the consumption in countries with same economic status of economical evolution. Overall requirements for this purpose in 2028–30 is estimated at approximately 5.30 million/tons from which domestic share could be only 1.99 million/tons. Generally, the genetically modified cotton (Bacillus thuringiensis) was acquaint in Pakistan...
Merica Slišković1, Meta Povž2, Marina Piria3,*, Goran Jakšić4, Ana Gracanin5 andGorana Jelić Mrčelić1
...total, 14 specimens were sampled by fishermen using sport fishing techniques. The age, condition, length-weight relationship (LWR), 20 morphometric and four meristic traits, were analysed. Fulton condition coefficient and LWR value indicated that sichel specimens’ were well adapted to the environmental conditions. Positive allometry in both males and females were observed (W = 0.004 L3.119). This represents the first LWR dataset ...
Asad Ullah1, Umar Sadique Khattak2, Sultan Ayaz1, Muhammad Subhan Qureshi2, Imad Khan1, Ibad Ullah Jan2, Irfan Khattak1, Raheela Taj3, Sahar Nigar4,    Naimat Ullah Khan1,Mumtaz Ali Khan5 and Muhammad Luqman Sohail6,*
...(Peshawar, Nowshera, Charsadda, Mardan and Swabi) located in the central zone of Khyber Pakhtunkhwa (KPK), Pakistan. A total of 2400 large ruminants were sampled by convenience sampling approach and tested by comparative cervical intradermal tuberculin test (CCIT) for detection of Mycobacterium bovis infection. Data regarding socio-demographic status, risk factors and farming practi...
Qudrat Ullah1,*, Huma Jamil1, Zafar Iqbal Qureshi1, Muhammad Saqib2 and Heinrich Neubauer3
...n. A total of 1000 blood samples (500 each from goats and sheep) were collected from animals kept at the respective livestock farms. An indirect-Enzyme Linked Immunosorbent Assay (iELISA) was used to detect anti-C. burnetii antibodies in the serum. Serological analysis revealed overall flock-level sero-prevalence of 92.3% which varied from 88.88% ...

Zulqarnain and Zafar Iqbal* 

..."text-align: justify;">Fusarium wilt of tomato is a devastating fungal disease with several infectious strategies leading to the complete death of plant. None of the strategies in controlling the wilt have shown fruitful results. Dual culture assay was employed is this study to identify the fungal species with their potent antifungal activity against the Fusarium wilt of tomato. Six distin...

Bushra Qadir1, Muhammad Asim2 and Mohammad Akmal1* 

...2013-14. Periodic growth sampling was taken by harvesting three pots of treatments starting in early spring and thereafter during tHEgrowth to the maximum five samplings to study roots and shoot growth. Averaged across varieties and extracts, results revealed that plant length (roots with shoot) was significantly (p<0.05) changed with a minimum at the thermal unit (TU) 677 to a maximum at TU 3257. Averaged across TU and e...

Bata Shalangwa Ishaku1*, Maimadu Abdullahi1, Dakwang Nalong1, Rengkat Jonah1 and Olabode Mayowa

...Five hundred (500) serum samples comprising of 300 pigs, 100 each of sheep and goats were collected and analyzed for Toxoplasma gondii antibodies (IgG) using latex agglutination test (LAT). Serum samples with LAT titer >10 IU/ml were considered positive. The study showed that 176 of the 500 samples analyzed were positive for Toxoplasma gondii antibodies (IgG) giving an overall prevalenc...
Moorthy S. Muthuswamy
... its enablers. This proposal further divides radicalization processes into primary and secondary ones; the former are identified with the onset of radicalization in a community, and the latter develop in communities with longstanding exposure to the phenomenon. A new analysis of a 2013 Pew Research Center report suggests that sharia’s popularity in a community can make religious leaders and their radical ideas highly influential, while in communities whe...

Muhammad Israr1*, Saeed Ullah1, Shakeel Ahmad2, Asif Yaseen3, Urooba Pervaiz4 and Nafees Ahmad5 

... by proportionate random sampling techniques form 90 sampled farmers through face to face interview survey method by filling a questionnaire. Farmer’s perceptions on agriculture LD were measured through the extent of severity scale in terms of LD dimensions and indicators resulted from water, tillage, soil, salinization, and overgrazing. Moderate (0.49) index of water degradation and...

Abid Hussain1*, Muhammad Azeem Khan2, Muhammad Anjum Ali Buttar3, Umar Farooq4, Muhmmad Islam5 and Zahid Ullah Khan1 

...s prefer to use higher dosages of Urea and low grade P-based fertilizers. This anomaly is negatively affecting productivity of major crops in general and high value crops in particular. Presently average weighted tax rate on all fertilizers is 5.83 percent. The imposition of uniform tax rate of 5% on all fertilizers, without cash subsidy on urea will reduce financial implication to Rs. 43 billion and at 2% tax rate financial implication will be Rs. 53.7 billio...

Muhammad Luqman1, Raheel Saqib2*, Mujahid Karim1, Khalid Nawab2, Abdur Rehman3 and Muhammad Yaseen1 

Yuan Li1, Liyan Zhang2, Karhoe Loh3, Ji Feng1, Xinqing Zheng1, Puqing Song1 and Longshan Lin1,*
...ions originated from the same refuge in the South China Sea. A historical demographic analysis indicated that P. minor experienced a recent population expansion during the late Pleistocene period. Due to the need of P. minor to adapt to the diverse habitats, unique haplotypes were ultimately formed under the differing pressures of natural selection. This study is expected to provide a basis for future research of the population genetics of P. ...

Ocheme Julius Okojokwu1*, Maryam Bisola Adebayo2, Bashiru Shafa Abubakar3, Ibrahim Abubakar Yusuf2 and Joseph Aje Anejo-Okopi1  

...us. A total of 326 blood samples were collected from consenting pregnant women who were attending antenatal clinic in Abuja, Nigeria. Structured questionnaire was used to obtain data on socio-demography and risk factors. The sera were obtained from samples were analyzed for IgG and IgM antibodies of human parvovirus B19 using SERION ELISA Classic Parvovi...

Inzimamul Haq1*, Shahid Sattar1, Bashir Ahmed1, Qamar Zeb2 and Amjad Usman

...data followed almost the same pattern as observed in case of leaf injury and dead hearts. Significantly lower yield was recorded in the control plots (3.54 ton-ha), while significantly higher yield was recorded in the plots treated with imidacloprid (6.61 ton-ha) followed by emamectin benzoate (6.10 ton-ha) treated plots. Results of the field test showed that significantly higher (88.0 %) parasitism of host eggs by T. chilonis was recorded in T. chilonis alone...

Saeed Ahmad Shah Chishti1, Mudassar Iqbal1*, Nusrat Parveen1, Kashif Nadeem1, Muhammad Iqbal1, Umbreen Shahzad2, Rana Husnain Shabbir1 and Muhammad Najeebullah1 

...text-align: justify;">Sarsabz, a high yielding, well adapted and bold-seeded pea variety was evolved by hybridizing two varieties Meteor and Lampo at Vegetable Research Institute, Faisalabad Pakistan. The variety was tested in replicated varietal/ station yield trials and multi-locational trials from 2001 to 2014. Sarsabz gave higher green pod yield (8.52 t/ha) than existing pea varieties ...

Ali Muhammad*, Yasser Durrani, Majid Suhail Hashmi, Ihsan Mabood Qazi, Muhammad Ayub and Saifullah 

...rties, which are indispensable for the functional and nutritional properties of foods. The aim of the study was to neutralize whey by addition of NaOH (0.1 and 0.01N) and NaHCO3 (0.1 and 0.01N) for fortification and enrichment of foods. However, addition of these solutions results in an increase in total solids and ash content of the whey sample The samples were normal whey (not treated), ...

Shaista Naz1*, Noor Paio Khan1, Naveed Afsar2 and Ashfaq Ahmad Shah3

...the country. For this, a sample size of 117 women respondents was selected through multistage sampling technique and data were collected from the selected respondents using a pre-tested semi-structured questionnaire through face to face interview. The collected data were subjected to analysis using descriptive statistics, frequencies, percentages, and rank orders. The results revealed that women were involved in buffalo, cat...

Asif Nawaz* and Muhammad Zafarullah Khan 

...t, Khyber Pakhtunkhwa. A sample of 234 out of total 543 Field Assistants were selected with the help of Sekaran Sampling Technique and the respondents were distributed in four Agro-ecological zones of the province with the help of proportional allocation sampling method. Data from the respondents were collected through pretested and validated interview schedule. Data was analyzed using SPSS Ver.20. Mean, standard deviation, ...

Monsif Ur Rehman1*, Hidayat-ur-Rahman1, Muhammad Iqbal2, Iftikhar Hussain Khalil1 and Zahir Shah3 

... mays L.) is the most versatile crop among cereals that grows well in a wide range of environments. Promising maize populations (and their hybrids) could be used as a source for inbred line development as well as cost-effective alternatives to single-cross hybrids. This study was aimed at determining combining ability and grain yield performance of S1 lines derived from ten maize populations. These populations were crossed in half diallel design and the result...

Aneel Salman1, Muhammad Iftikhar ul Husnain1, Inayatullah Jan2*, Muhammad Ashfaq2, Mudassar Rashid1 and Usman Shakoor1 

Amjad Ali1, Abbas Ullah Jan1, Lal Almas2, Noor Piao Khan1 and Khurran Nawaz Saddozai

... growers in district Charsadda was worked out with frontier model. Randomly 118 sugarcane growers were interviewed during crop season 2014-2015. For survey data a pre-tested structured questioner set according to objectives of the study was used. Coefficients of parameters were calculated by maximum likelihood estimation techniques. Per acre total yield, land rent and per acre inputs cost (labor hired, seed, tractor hours, irrigation, Urea, DAP, FYM and pestic...

Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq

...ondii (T. gondii). Blood samples of chicken (n=71), were collected from Cholistan areas of Bahawalpur (Punjab), Pakistan. Serum samples were tested for toxoplasmosis with a commercial latex agglutination kit (Antec Diagnostic products, UK). The overall prevalence was 26.76%. The significant (P<0.05) higher seroprevalence was observed in males (34.28%) than in females (19.44%). For analysis of hematological parameters bloo...

Sabahat Noor*1, Shaukat Ali1, Hafeez-ur-Rahman1, Farhatullah2 and Ghulam Muhammad Ali1 

...resses, drought and soil salinity have the most adversarial effect on wheat production. In this study the T2 transgenic (At-DREB1A) and non-transgenic line of lasani-08 from single transformation event was exposed to 15 days drought and different levels of salt concentrations separately. Four parameters i.e. sugar contents, proline contents, chlorophyll ...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
...crophomina phaseolina, Fusarium oxysporum. The metal (III) complexes are highly active under permissible limits.
...
Naila Chand1, Shamsullah1, Rafiullah1, Rifat Ullah Khan2, Muhammad Mobashar3, Shabana Naz4,*, Ebrahim Rowghani5 and Murad Ali Khan2
...the impact of mannanoligosaccharide (MOS) on the growth performance, weight of lymphoid organs and intestinal histomorphology of broilers during starter phase. A total of 180 day-old broiler chicks were distributed into 3 treatments, designated as MOS-0, MOS-50 and MOS-100 having MOS at the rate of 0, 50 and 100 g/kg respectively. Each treatment was further replicated 3 times having 10 chicks per replicate. Treatment MOS-0 was kept as control and the birds in ...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
..., General Hospital, Al-Ehsan welfare Hospital, Mughal eye and Mayo Hospital with the help of ophthalmologist. The main purpose of such type of research was directed to find the prevalence of Macular corneal dystrophy in the families of Lahore. The techniques used for the diagnosis of MCD were visual Acuity test by Snellen chart, phoropter, slit lamp biomicroscopy, topography, keratometer and pachymetry. In this study, 50 patients of MCD were identified among w...
İnan Kaya1,*, Muhitdin Yılmaz2, Müge Mavioğlu Kaya3,Abdulsamed Kükürt4 and Mahmut Karapehlivan5
...ely. The blood and liver samples were taken from the fish for biochemical studies at the end of the study period. Oxidative stress index (OSI) in plasma were evaluated as well as AST and ALT levels in the liver. Plasma OSI and liver AST levels in the groups with carbaryl and 2,4-D were compared with the control group, and they were found to be significantly higher. As a result, it was concluded that carbaryl and 2,4-D administered under of LC50 valu...
Fehmeeda Fatima, Asif Nadeem* and Maryam Javed
... ibex. In this research, samples of Sindh ibex were collected from Kirthar National Park, Sindh, Pakistan, the gene of interest was amplified, PCR products were sequenced and data was analyzed. The results of genetic diversity indicated that Parsimony informative sites were found to be 11 while Singleton variable sites were not detected. The total number of haplotypes (h) was found to be 17 and haplotype diversity (Hd) was 0.862. Nucleotide diversity (pi) was ...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi2*, Zillay Mariam1, Nadeem Sheikh1,2*
...atment prior to its disposal.
...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2

 

...kers. Blood and mosquito samples were collected. Blood samples were processed through complement fixation test and RT-PCR, while mosquito samples were processed through RT-PCR. Overall prevalence of bovine ephemeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more prone to disease. In the case of buffalo breeds, prevalence ...
Mouslim Bara1,2,* and Luciano N. Segura3
...and Shannon index. Field samplings indicated that bird abundance was frequently higher than 1000 individuals, which suggests that this site is an important refuge of water birds in the Guerbes-Sanhadja wetlands complex. Two key conservation species were observed regularly in the Garaet: near threatened Ferruginous Duck (Aythya nyroca) and endangered White-headed Duck(Oxyura leucocephala). Our results highlight the importance of preserving the Gar...
Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
...lia tears to achieve satisfactory microbiological quality and public health safety. Hence, examining the effect of gamma-irradiation on the contents of Boswellia oleo-gum resin and also investigating the role of gamma-irradiate frankincense aqueous extract (GFAE) against ISO-induced MI in rats were the two aims of this study. The total phenolic content and total flavonoids of frankincense has been significantly in...
Farah Mansha, Abdul Rehman*
...the basis of chitinase assay and diameter of zone of hydrolysis. All the isolates were identified as Bacillus sp. on the basis of biochemical tests, and showed optimum growth at 37°C and pH of 7. All the bacterial isolates showed highest enzyme activity at pH 7. Isolates H4 and M4 showed maximum enzyme activity at 55°C while H1 showed highest activity at 70°C. Chitinase activities exhibited by H1 and M4 are stimulated by the presence of Zn
Asima Bano1, Hafiz Muhammad Tahir2, Hira Sherawat3, Muhammad Mutlib4, Muhammad Arshad5, Muhammad Akram Qazi6, Sajida Naseem5, Rabia Ishaq1, Iram Liaqat2 
...enzyme estimation plasma samples from the patients suffering from breast cancer and age matched healthy individuals (control) were collected. The results revealed decline in the level of these enzymes in breast cancer patients as compared to healthy individuals because of this decline the overall level of protein was also found to be decline. It is concluded from the study that these enzymes can be used as biomarker in the diagnosis of breast cancer. 

Umair Riaz1*, Muhammad Ali Kharal2, Ghulam Murtaza3, Qamar uz Zaman4, Sana Javaid4, Hina Ahmed Malik3, Humera Aziz3 and Zafar Abbas1 

...best option to cope with salinity, ion toxicity, drought and heavy metal stress. 

...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

...ngredients including polysaccharides, vitamins, proteins, essential amino acids, carotenoids, fatty acids like linoleic acid and linolenic acid, lignans, minerals and various phytochemicals. Due to the presence of two unsaturated fatty acids, these oils can reduce their biological activity on heating, volatilization, and other parameters like oxidation and UV rays. This limits the commercial application of these oils. To ove...

Muhammad Latif2, Muneer Abbas1*, Faisal Hafeez2 and Dilbar Hussain2 

... spinetoram 120 SC, spinosad 240 SC, bifenthrin 10 EC, cypermethrin 10 EC, chlorantraniliprol 20 SC and flubendiamide 480 SC were applied on brinjal and okra against these pests with five replications. Data was recorded after 24 hrs. 72 hrs. 7 and 10 days after spray application. Results indicated that all the insecticides gave significant control of both pests up to 72 hrs. of spray application. After this pest population was started to increase. In L. orbona...
Ghazunfar Rashid1, Muhammad Avais1, Syed Saleem Ahmad1, Muhammad Hassan Mushtaq2, Rais Ahmed3,*, Mahboob Ali1, Muhammad Naveed-ul-Haque4, Mehtab Ahmad5Mumtaz Ali Khan1 and Naimat Ullah Khan6
...ys (Makai), Avena sativa (Jai), Brassica rapa (Shaljam) and Brassica Campestris (Sarson) were estimated twice a day i.e. early morning and afternoon. The fodder samples were collected from different villages of Okara, Pattoki and Ravi areas of the Province Punjab. Nitrate contents of different parts of the fodder plants were estimated qualitatively through the Diphenylamine Filed Test (DFT) an...

Jafar Aqeel Naqi1, Abdul Mateen1, Dilawar Hussain2,*, Hafiz Muhammad Tahir2, Sajjad Hussain1 and Abuzar Tabasum1 

...ect of garlic (Allium sativum) as a natural feed ingredient on growth performance and immune response in Nile tilapia (Oreochromis niloticus). A total of 150 juvenile’s tilapia were taken randomly and divided into five experimental units (10 fishes per unit) with three replicates. Fish was hand feeded containing five different levels of garlic (T1, control) 2.5 (T2), 5.0 (T3), 7.5 (T4), and 10g/50...
Tariq Mahmood1,*, Ijaz Ahmad1, Faraz Akrim1, Abdul Hamid1, Muhammad Waseem1, Abid Hussain1 and Muhammad Sajid Nadeem2
... found at five different sampling sites; nest location was mostly on sloping areas under sanatha Dodonaea viscosa shrubs. Nesting material consisted of dry leaves of annual grass Poa annua, small twigs of bushes and downy feathers. The depth of nest ranged between 5-10 cm. Clutch size ranged from 8-20 eggs while the incubation period was found to be 22-24 days. Hatching succe...
Tevfik Ceyhan1,*, Osman Samsun2 and Okan Akyol1
...tribution of all garfish samples ranged from 28.8 to 51.6 cm TL and from 26.6 to 177.2 g, respectively. Female: male ratio was 0.3:1. The LWR parameters (a, b, r2) were computed as 0.0009 ± 0.50, 3.040 ± 0.32 and 0.902 ±0.40, respectively. The age of garfish was between 1 and 4 years. The estimated von Bertalanffy growth parameters with standard errors were L= 55.74 ±26.75 cm, K= 0.28 ±0.42...
Muhammad Mohsin1, Dai Guilin1,*, Chen Zhuo1, Yin Hengbin1 and Muhammad Noman2
... in Pakistan, it is necessary to evaluate fishery status and their economic implications of major commercially hunted fish species. In this study maximum sustainable yield (MSY) of Carangoides fishery from Sindh, Pakistan is estimated by using catch and effort data. For data analysis, two specialized fishery software CEDA (catch and effort data analysis) and ASPIC (a stock production model incorporating covariates) were employed. Three surplus production model...
Zafar Iqbal*, Farah Ansar, Zil-e-Huma
...Rhizopus and Fusarium. Ten bacterial genera were also isolated; Aeromonas, Pseudomonas, Streptococcus, Enterococcus, Acinetobacter, Bacillus, Moraxella, Lactobacillus, Staphylococcus,. Carnobacterium. There has not been a single case of topically acquired infection among students, laboratory staff and the author throughout the whole period due to contact with these fish. One reason for this is the use of sa<...

Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3  

...stify;">Radish (Raphanus sativus L.) seed oil extract was analyzed for the presence of potential non-volatile phytochemicals and in vitro antibacterial activity against two phytopathogenic bacterial species (Clavibacter sp. and Xanthomonas sp.) and a human pathogenic bacterium (Escherichia coli) in 2016. Experiment was carried out by using complete randomized design with three replications in vitro. Phytochemical analysis reveals that the methanolic fraction o...

Muhammad Kashif Asghar1, Muhammad Aqeel Sarwar1,2*, Shahid Riaz Malik2, Waqas Ahmad3, Sumaira Zareen4, Safdar Ali5 and Abid Ali6 

...rsity of Agriculture, Faisalabad. The achenes of two different sunflower hybrids i.e. Hysun-33 and FH-331 were subjected to different priming treatments including hydropriming, osmopriming (with 1, 0.1 and 0.01 mM B) using boric acid as a source of B. The experiment was laid out in randomized complete block design with factorial arrangement in triplicate run. Results from the experiment revealed that all the seed treatments significantly improved achene yield,...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...ar examination. In blood samples of dogs with visceral leishmaniasis, levels of malondialdehyde, total antioxidant capacity, glutathione, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines (TNF-alpha, IL-1β) were determined and compared with the healthy controls. Significantly high levels of plasma malondialdehyde, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines and significantly low levels in total antioxidant capacity and ...
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
...nt of SCM in District Faisalabad of Pakistan. For this purpose, a total of 384 goat milk samples were screened for SCM through surf field mastitis test (SFMT) and mastitis-positives cases were further investigated for isolation of S. aureus using standard procedures. Coagulase gene was PCR amplified from the clinical isolates to categorize them into Coagulase positive Staphylococci (CPS) and coagulase negative ...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro
...nitial examination semen sample having motility ≥ 70% and morphology live dead ratio ≥ 80 % were diluted in Tris based extender consist of 0,2,4 and 6mM concentration of selenium. Four hours after dilution chilled semen was examined for post chilled semen quality, motility% (74.75±1.9), morphology% (86.62±1.2), membrane integrity% (87.87±0.6), live dead ratio% (87.62±0.7). After that samples...

*Nageen Fatima1, Sadia Murawwat1 and Syeda Ameena Rizvi1

EVALUATION OF IMAGE COMPRESSION TECHNIQUES: AN IMPLEMENTATION OF TRUE COMPRESSION ON 2-D IMAGE
...chniques are reliable to save hardware memory consumption but having low CR ratio. In lossless techniques, CR ratio is higher and it uses more space to store compressed image. Proposed comparison between lossy and lossless techniques is supportive in choosing an optimal technique for image compression and facilitates the process of an efficient compression.

...

 *Muhammad Waseem1, Dost Mohammad Baloch1, Ghulam Khaliq1,
M. Rashid1, Qurban Ali2 and Mustajab A. Khan3

INTENSIFICATION IN BAMBOO (Bambusa bambos L.) TRAITS IN RESPONSE TO ORGANIC AND CHEMICAL NITROGEN ALONG THE COAST OF ARABIAN SEA
... of 2m. The bamboo (Bambusa bambos L.) plants were grown with four fertilizer sources i.e. S = Control (0) kg N ha-1, S = urea (120 kg N ha-1), S = Farm Yard Manure (FYM 1% N), S = 0 1 2 3 Combination {50% N from urea (the recommended dose) + 50% N from Farm Yard Manure (FYM)}. A recommended dose of nitrogen was used as chemical source and applied at the rate of 120 kg N ha-1. Significant differences were reported for studied traits in response to treatments. ...

Nadia Sharif1, *Neelma Munir1 and Shagufta Naz1

MICROBIAL PRODUCTION OF ALGINATE
...lginate is a natural polysaccride that occur as 30 to 60% structural component of brown algae. Alginate favorable properties like ease of gelation and biocompatibility promote its applications in engineering and bio medical science.

...

 Muhammad Akram and Faheem Aftab

THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
... of initial culture. The same cultures were further maintained and subsequently obtained with 20.6 mm shoot length and 7.2 average number of leaves for another 10 days. Similarly, all other TDZ’s concentrations below 1 uM were also effective in bud breaking and development of shoots with green and vigorous growth. The frequency of bud break was significantly reduced with the increasing concentrations of TDZ. There were 2 phases of callus formation on the...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water

Hareem Mohsin, Azka Asif*, Warda Fatima, Sarooj Nadeem, Anjum Nasim Sabri

Assessment of biocalcification by the water absorption test using microorganism from oil contaminated site
...as studied on the cement samples. The cement samples were treated with urea and calcium salt (calcium carbonate) that act as a source of calcium along with the microorganism under optimum culture conditions. Alkaline conditions produced by the activity of the enzyme urease allowed the reaction between calcium and carbonate ions that leads to the precipitation of the calcium carbonate. The ...

 Hafiz Muhmmad Tahir1*, Rabia Yaqoob2

Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
...ads. One hundred seventy samples, including both fresh and up to five years old tissue samples, were compared. Taq DNA polymerase was found to be less efficient compared to the PuRe Taq Ready-To-Go PCR beads for amplification of the 12S rDNA gene. However, difference in the efficiency of both procedures was not statistically significant for the 16S rDNA gene. Furthermore, Taq DNA polymerase was found only efficient for fresh...

 Tanzeela Riaz*†, Farah Rauf Shakoori, Syed Shahid Ali

Toxicity of phosphine against tolerant and susceptible populations of Trogoderma granarium collected from Punjab, Pakistan

Muhammad Babar Khawar, Nadeem Sheikh*

Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
...ol group (4ml/ kg normal saline), Group 1 (4ml/ kg leachate) and Group 2 (4ml/ kg 1:10 diluted leachate). All the animals received the treatment through intraperitoneal injection. After 24h of the injection, all the animals were sacrificed and blood was collected, sera were separated and processed for further analysis. Serological analysis revealed that leachate induction leads to significant positive variations in level of ...

 Mian Azhar Ahmad1†*, Nawab Mohammad Khan2, Asmatullah3

Anatomical variations of hepatobiliary triangle in patients operated laparoscopically for gallbladder diseases from Lahore and Sahiwal
...cholecystectomy was necessary; the most common cause was unclear anatomy. Laparoscopic cholecystectomy pivots around hepatobiliary triangle. Current study was aimed to assess anatomy of hepatobiliary triangle and its variations in the patients using a laparoscope. It was a descriptive prospective cross-sectional study. Random sampling technique was employed to record the relevant information data. During the course of this s...

 Muhammad Tahir Waseem, Muhammad Awais Sarwar, Rana Manzoor Ahmad, Abdul Majid Khan*

Newly discovered fossil remains of Selenoportax vexillarius from Hasnot, locality of Siwaliks of Pakistan
...us Selenoportax. All the samples are collected from well exposed strata of Late Miocene of Hasnot, district Jehlum, Pakistan. All The specimens are identified and characterized on the basis of morphometric comparison and morphological similarities and described as Selenoportax vexillarius. The molars have divergent styles and moderately developed median basal pillars while the crown in all specimens is slightly narrow at the...

 Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2

Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...rum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT), Alkaline Phosphatase (ALP), total protein, bilirubin, globulin, creatine and uric acid along with reduction of SGOT/SGPT, Blood Urea Nitrogen (BUN), urea and albumin as compared to control. Treatments with JFE after Cr+6 exposures significantly improved the hepatic and renal functional profiles possibly by partial liver rehabilitation a...

 Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4

Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
...Water, sediment and fish samples were collected on monthly basis from upstream and downstream of head Trimmu, river Chenab. Throughout the study period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maximum Cr concentration 81.72±2.54 was ...

 Shagufta Andleeb*

Teratogenic potential of pyrethroids: a review
...owers of pyrethrums (Chrysanthemum cinerariaefolium and C. coccineum) in late 1900s. An extensive data on multiple aspects of genotoxicity, carcinogenicity, mutagenicity, reproductive toxicology and neurotoxicology is available except teratogenicity. This review concludes the teratogenic potential of sublethal doses of different types of pyrethroids in various groups of animals in systematic way. On line available data interpretation through Pubmed Central NCB...

Durr-e- Samin Tahir*, Waqar Nasir, Sarah Bushra, Fatima Batool

Height trends in the population of Rabwah, district Chiniot, Pakistan and comparison with WHO standards
...rowth rate is almost the same up to 13 years, but it is higher for males after 13
years age. The female height trend revealed non-significant increase beyond 16 years, while, for males this trend
increased until 19 years. According to mid-parental height plots, 73% male and 84% female data lies within +3 inches
range of mid-parental heights. The comparison of percentile curves of local height trend and WHO curves indicate
that local...

 Haji Muhammad1, Zafar Iqbal1, Tanveer Akhlaq2

Length-weight, length-length relationships and condition factor of fishes of family cyprindae from the Indus River, Pakistan
... family cyprinidae. Fish samples were
collected on a monthly basis from August 2013 to July 2014 at Taunsa barrage from the Indus River, Punjab,
Pakistan. The values of parameter b for LWRs ranged from 2.16 – 4.10 suggesting the isometric and allometric
growth pattern in fishes of family cyprinidae. The results of K factors ranged from 0.645 – 1.836 also determine both
negative growth ...

 Muhammad Moosa Abro1, Ali Murtaza Dharejo2, Muhammad Munif Khan2, Nadir Ali Birmani2, Saima Naz2*

Description of first record of Petasiger exaeretus Dietz, 1909 (Trematoda: Echinostomatidae) in avian host from Pakistan.
...x, pre-acetabular cirrus sac, tandem and contiguous testes, short uterus, spherical and pretesticular
ovary. P. exaeretus is a common parasitic species of Cormorants. However, it has not been reported from
Pakistan in Little Cormorant, Phalacrocorax niger prior to the present study.

...

Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*

Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
...y treatment (n=3). Serum samples were processed
for the estimation of total protein and albumin and aminotransaminases activity. The sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases were noted in ...

 Saima Naz*, Adil Ali Rajpar, Abdul Hameed Chandio

New Records of some Phthiraptera (chewing lice) of birds from urban areas of Hyderabad, Sindh, Pakistan
...domestic fowls; Brueelia saliemi (Ansari, 1957), Colpocephalum fregili (Denny, 1842)
and Myrsidea splendenticola (Klockenhoff, 1973) on house crow; Anaticola crassicornis (Scopoli, 1763) from wild
goose; Brueelia nebulosa (Burmeister, 1838) and M. eurysternus (Burmeister, 1838) from common myna and bank
myna; Neopsittaconirmus lybartota (Ansa

 Abbas Ali1, Muhammad Altaf2*, Muhammad Samar Hussain Khan3

Winter survey of birds at Keti Bunder, district Thatha, Pakistan
...r are decreasing with passage of time when the data were compared with the
previous research due to anthropogenic impacts especially pollution impact.

...

 Abir Ishtiaq, Muhammad Naeem*

Length-weight relationships and condition factor for farmed Catla catla (Hamilton, 1822) from southern Punjab, Pakistan
...dy weight (W) of studied samples have been calculated as 39.29±0.23 cm and 827.43±14.55 g,
respectively. For LWRs, specimens of C.catla were collected from fish ponds and grouped into three categories on
the basis of the feed offered to them which contained 15%, 20% and 25% crude protein (CP). The regression
estimates for LWRs were highly significant (P <0.001) with coefficient of determination, r2-values being >0.930 i...

 Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3

Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...e blood and liver tissue sampling was done after 96hrs for histology and
biochemical analysis [alanine amino transferase (ALT), and aspartate amino-transferase (AST)]. Liver histology
revealed that necrosis, nuclear pycnosis, hypertrophy of hepatocytes, vacuolization, nuclear atrophy and congestion
of blood vessels were observed as compare to control group. This result was also supported by the significant
increase in levels of hepa...

Dilara Abbas Bukhari1, Abdul Rehman2*

Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
...tes were detected in the sample by GC/MS. The detected metabolites could be classified into chemical groups of 2 esters, 4 alcohols, 3 benzoic acids, ketone, aldehyde, phenyl and amide groups containing compounds. Some of these may be classified into aroma containing compounds.

...

Sadaf Niaz1, Masroor Ellahi Babar2, Tanveer Hussain2, Asif Nadeem1, Misbah Hussain1, Riffat Mehboob3*, Fridoon Jawad Ahmad3

Mutation analysis of RING1 domain of Parkin in early onset of Parkinson’s disease in Pakistani patients-a pilot study
...otein was performed in a sample set of 30 patients (selected from different areas of Punjab, Pakistan) to find out
any Single Nucleotide Polymorphism (SNP).No SNP was detected in RING1 domain that could be related to the
disease. The data suggests that no genetic predisposition in RING1 domain may be responsible for the occurrence of
disease in local population. It may be due to genetic changes in any other part of the gene or due to other...

 Haniya Mazhar1, Naaz Abbas2, Zahid Hussain3, Amir Sohail4, Syed Shahid Ali4*

Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
...ruit peels and waste oil samples used in this study, the maximum lipase production was observed with soya bean meal, banana peel and Shan oil waste, respectively.

...

 Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1

Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
... uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution. Rats of 12h and 24...

 Zubair Ahmed#, Muhammad Farhanullah Khan, Habiballah Rana*

Toxicological effects of Haloxylon recurvum Bunge ex Boiss (Khar Boti) whole plant extract and novel insecticide chlorantraniliprole against maize weevil, Sitophilus zeamais Motschulsky
...r /> Contact toxicity assayed by topical application showed that 3.6% concentration of
H. recurvum plant extract and 0.00896% concentration of chlorantraniliprole
induced lethal effects in S. zeamais. Probit analysis revealed LD50 value for H.
recurvum plant extract were 16.47 μg/insect and chlorantraniliprole were 0.0358
μg/insect, after 24 hours. The result revealed that H. recurvum plant extract had
insecticidal pot...

 Waqas Ali, Arshad Javid, Ali Hussain, Syed Mohsin Bukhari

Public attitude towards amphibian and reptiles in district Kasur, Punjab, Pakistan
...s mindset lead to unnecessary killing of species. Among all the
herpetiles, snakes are most disliked taxa and killed by the locales. Conservation
education and awareness campaigns are recommended to avoid unnecessary
killing of the amphibians and reptiles of the study area.

...

 Mohsin Arshad1, Muhammad Irfan*2, Asad-Ur-Rehman1, Muhammad Nadeem3, Zile Huma3, Hafiz Abdullah Shakir4, Quratulain Syed3

Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification
...e enzyme was applied for saccharification of
agricultural wastes and maximum saccharification (11.45%) was observed in
bagasse. These results suggested its potential utilization in particular biofuel
industry.

...

Madeeha Arshad, Asmatullah, Chaman Ara

Embryotoxic effects of sodium arsenate in Mus musculus
... smelting and pesticide usage. Inorganic arsenic
(sodium arsenate) other than general lethality additionally can be a teratogenic
agent. Present research work is focused on the teratogenic impacts of sodium
arsenate given to gravid mice (Mus musculus). Different doses of sodium arsenate
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one c...

Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1

Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis

Nabila Roohi, Mehjabeen, Samina Ashraf*

Effects of cigarette smoking on serum proteins profile in male active and passive smokers
... passive smokers. Blood samples of 180 cigarette smokers were collected from
different locations of Lahore and 60 healthy non-smokers from University of the
Punjab, Quaid-e-Azam campus Lahore, in terms of comparable age, height, weight
and socioeconomic set up. Serum protein profile was analyzed using commercially
available kits by using automated clinical chemistry analyzer. Serum total proteins
have shown a significant dec...

 Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1

Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...tion
of virus, 21 samples were collected, out of which 8 (40%) were positive for H9. The
virus titer was ranged from 1:64 to 1:512. The body weight gain of FO
supplemented broilers was higher than broilers receiving cooking oil (CO) while the
FCR of FO treated broilers was significantly lower than broilers receiving diet with
CO before virus inoculation, furthermore a significant difference was observed in
FCR after ch...

Muhammad Asghar Hassan1, Imran Bodlah1* , Ayesha Aihetasham2 

First record of the oriental species, Saltella setigera Brunetti, 1909 (Diptera: Sepsidae) from Pakistan

 Khurram Shahzad1*, Muhammad Naeem Khan1, Farhat Jabeen2, Nasreen Kosour3, Muhammad Sohail1, Muhammad Khalil Ahmad Khan1, Munir Ahmad1

Bioaccumulation of manufactured titanium dioxide
... tilapia (Oreochromis mossambicus). Fish were exposed to three doses of
nanoparticles suspensions T0 (no dose administration), T1 (0.5 mg/L), T2 (1.0
mg/L) and T3 (1.5 mg/L) for 14 days. After 14 days, animals were slaughtered and
organs (gills, liver and muscles) were freeze dried. Freeze dried tissue samples
were digested with the help of nitric acid (HNO3) and perchloric acid (HClO4). Acid

 Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2

An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
...
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combinations thereof to
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and physical features, making it prone to
enzymatic attack for
Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*
...ated from soil and water samples from oil industry, mobile oil filling areas and kitchen wastages were screened on tributyrin agar and rhodamine agar plates. The strain PCSIR NL-37 was identified as Bacillus cereus following morphological, biochemical and molecular characterization. Best medium for lipase production was olive oil and mustard oil cake and the best carbon sources were fructose and maltose whereas yeast extract was found to be the best nit...
Muhammad S. Waqas, Ali A.Z. Shoaib, Xinlai Cheng, Qianqian Zhang, Asem S.S. Elabasy and Zu-hua Shi*
...ated females produced ovisacs, while they neither produced eggs nor gave birth any crawlers in the ovisacs. Unmated females lived for longer durations than the mated ones. Sexual reproduction with short lived males may imply potential for control practices that target males; for example, application of sex pheromone for trapping and killing male, or spraying of chemicals during the time of male activity, may be an effective ...
Atiq-ur-Rehman1,2,*, Abida Latif1,*, Rukhsana Anwar1, Nasir Abbas1, Sajid Nawaz2 and Hamid Turab Mirza3
...ha;-amylase inhibitory assay. Different concentrations of fractions (20-100 µg/ml) were subjected to in vitro α-amylase inhibitory assay. Assessment of anti-hyperglycemic potential of the most active chloroform fraction exhibiting in vitro α-amylase inhibition was carried out in Wistar albino rat models. Changes in postprandial levels of blood glucose in rat models (normal and diabetic) were e...

Faheem Ali1*, Muhammad Naeem Arbab1, Abdul Basit2, Muhammad Kashif Khan1, Gulzar Ahmad1 and Muhammad Amir

... must be balanced by the same number of leading VARs in order to ensure unity power factor, thus avoiding penalties on agricultural industry. Switched Capacitors and Voltage Regulators are used to cope with such a situation. Regulators results in higher line currents thus resulting in excessive losses in addition to voltage sag problems. On the other hand, static capacitors can tackle the reactive VARs, but in steps only. Th...

Muhammad Shahzad Akbar, Muhammad Zeeshan Majeed* and Muhammad Afzal 

...at, pymetrozine and spinosad were least effective against O. obesus termites. Conclusively, based on the results of this study, chlorantraniliprole, chlorfenapyr, pyriproxyfen, triflumuron and indoxacarb are recommended to be incorporated in future integrated pest management programs against subterranean termites. 

...

Akshay Sharma*, Mohit Mahajan, Madhumeet Singh and Pravesh Kumar 

...is of different amniotic sacs and corpus luteum on one ovary. Color Doppler study was done to assess the vascularity and functional activity of corpus luteum present on the left ovary. Ultrasonography was employed at weekly interval to judge the viability of embryo (up to 42 days), fetus (after 42 days) and late embryonic mortality, if any. Sexing of the twins was done on 56th and 70th day post artificial insemination and two male twins were confirmed on the b...

Abid Muhammad Shah1, Safdar Ali1*, Ijaz Ahmad2, Gulzada Wazir1, Obaidullah Shafique1, Muhammad Amir Hanif1, Bashir Ahmad Khan3 and Sumaira Zareen4 

...nd out the remedy of the said issues a field experiment was conducted at University Research Farm, Chakwal Road, Rawalpindi during 2015-16 to investigate the effect of different herbicides and sowing methods on wheat productivity and population dynamics of weeds. Different wheat sowing methods (S) were Broadcasting, Line sowing, Ridge sowing, Bed sowing and herbicides used were Ally Max, Clean wave, Starane M. and Buctril Super 60 Ec. The experiment was laid o...

Saman Fatima* and Shazia Iram 

...uality. About twenty six sampled fruits of Citrus reticulata having damaged skin were obtained from the orchards of Sargodha. The samples were classified into groups on the basis of physical appearance of lesions on peal and the disease severity was then identified. Both classical and molecular method was used for identification of pathogens. Under classical approach the infected sections from the skin of kinnow was utilized...

Umair Talib1, Ijaz Ashraf2, Robert Agunga1 and Saleem Ashraf3* 

...l degradation and at the same are impacted by it. More than 80% of the farmers in Pakistan are resource-poor or practice subsistence farming which degrades the environment. Therefore, there is an urgent need for climate smart extension education for these farmers. The main purpose for this study was to examine environmental degradation/climate change associated problems faced/ perceived by resource-poor farmers and ways adopted to mitigate them. The specific o...

Neelum Andaleeb1*, Munir Khan1, Syed Attaullah Shah1 and Rahmat Ullah2 

...ck farming. A multistage sampling technique was used to collect a sample of 274 livestock farm households from six union councils of district Mardan. Data were collected through face to face interviews from female members of the selected livestock farm households in 2015-16. Data were analyzed by ordered logit model to determine factors affecting households welfare, measured as daily per capita income level. Variables, such ...
Aiguo Zhou1,3,4, Shaolin Xie1,4, Zhenlu Wang1, Lanfen Fan1, Yanfeng Chen2, QiaoYe1, Fang Zeng1 and Jixing Zou1,*

 

...hat they belonged to the same species, but not subspecies at the molecular level. Moreover, take Lateolabrax maculates and Epinephelus coioides as outgroups, molecular phylogenetic tree showed that all the haploids gather together as a branch and crossing each other. These indicated that the white type Channa argus should be regard as an albino of biocolor type.
...
Ajmal Khan* and Rehan Ullah
... 2013 to December of the same calendar year to cover all the four seasons. The data of this descriptive study was processed and analyzed to investigate the prevalence of malaria and its specific aetiologic agents in suspected patients reported to various laboratories of Agency Head Quarter Hospital Khar, Bajaur Agency. In this study, a total number of 9720 suspected individuals were examined; among which 1273 (13.1%) were found to be positive for malaria infec...
Faiz Muhammad1,2, Canfeng Dou1, Zhen-ming Lü1,*, Li Gong1, Xun Du1 and Muhammad Shafi3
...ls using mtDNA ATPase 6, sampled from Dalian, Dongshan, Nantong, Qingdao, Shanghai, Wenzhou, Xiamen, and Zhoushan China. Based on 672 bp fragment of Mitochondrial DNA subunit ATPase 6, thirty haplotypes and 140 polymorphic sites were noted, whereas (0.740, 0.048) high and low haplotype and nucleotide diversity observed. The data was further tested with AMOVA that validates 91.24% variation among populations and 8.76% within population. Genetic fixation in...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...examination of the tumor sample. In all cases, tumor resection was performed followed by cauterization of the remaining surface with silver nitrate. No postoperative complications or local recurrence were reported by owner of each case.
...

Muhammad Shahzad Khattak1*, Amjad Khan1, Mahmood Alam Khan1, Waqas Ahmad2, Shafiq ur Rehman3, Mohammad Sharif4 and Sajjad Ahmad1 

...a, Mangla, Marala and Warsak hydrological stations for the period 1962-2011. A drought spell starting from 1999 and ending in 2002 was observed for all stations and is considered to be the worst drought in the history of Pakistan. Analysis of historical streamflow data indicated an increased occurrence of droughts in the last 12 years, which may be attributed to climate change. Extreme drought events were observed at Tarbela during October-September and at Man...

Fouzia Tabssum1, Qamar uz Zaman2*, Yinglong Chen3,4, Umair Riaz5*, Waqas Ashraf6, Ambreen Aslam2, Nusrat Ehsan2, Rab Nawaz2, Humera Aziz7 and Shamim ul Sibtain Shah8 

...plied proline to enhance salt tolerance of two rice cultivars with contrasting salt tolerance: Super basmati (salt susceptible) and Shaheen basmati (salt tolerant) in both nursery and field conditions. For pot experiment, salinity stress was imposed by applying 50 mM of NaCl solution to the soil daily for three continu...

Mubashir Ahmad Khan*, Nazakat Nawaz, Muhammad Arshad, Muhammad Ayub Khan, Tahira, Doulat Baig, Ihsan Ullah Khan and Shamim ul Sibtain Shah 

...ent comprised 98 local sesame genotypes including a standard cultivar (TS-3), was conducted during the Kharif seasons of 2011 and 2012 in the research fields of Oilseeds Research Program, Crop Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan. Data were recorded on days to flower initiation, days to flower completion, reproductive period, days to maturity, pod filling period, plant height (cm), number of branches per plant, number ...
Fatih Korkmaz1, Veysel Parlak2, Özgür Kaynar3, Arzu Ucar2, Gonca Alak1,* and Muhammed Atamanalp2
...ardamomum), and chitosan coatings on the rainbow trout fillets were evaluated based on the protein profile, bacterial content (total aerobic mesophilic, psychotropic and lactic acid bacteria, Pseudomonas and Enterobacteriaceae), lipid peroxidation (TBARS), total volatile basic nitrogen (TVB-N) and pH values on quality during 12 days. The decrease in bacterial growth activity, physicochemical values (TVB-N, TBARS and pH) and prolonged shelf life in a...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...oup (P < 0.05). Immunisation with ptCS/2SS-asd promoted the growth of rams similar to the effect of the simple co-injection of CpG and SS DNA vaccine. Thus, the fusion type of CpG adjuvant is an effective, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
...
Abderraouf Chouaib Rebbah1, Mohcen Menaa2,*, Salah Telailia3,Menouar Saheb1 and Mohamed Cherif Maazi2
...of dissimilarity between sampled habitats. The dissimilarity between pine woodlands and mixed oak-pine forests was about 50%, in general, and produced by differences in abundance of Common Chaffinch Fringilla coelebs, European Serin Serinus serinus, House Sparrow Passer domesticus, European Greenfinch Chloris chloris, Spotted Flycatcher Muscicapa striata, andCommon Blackbird Turdus merula. The differences between pine ...
Shaher Bano1, Sarah Ghafoor1,* and Nadia Naseem2
... in ductal epithelium of salivary glands. The development of salivary glands is under the influence of various hormones including Triiodothyronine hormone (T3). Submandibular gland of rat is an excellent model to study salivary gland organogenesis. The study is designed to understand T3-mediated regulation of TGF-α expression during postnatal saliv...
Xiao-Ying Ren1, Di Zhang2 and Wan-Long Zhu1,*
...anterior tip of suture nasal and premaxilla, anterior tip of suture between nasal and premaxilla, anterior and posterior most ventral points of the upper incisor alveolus, and coronoid process, angular process ascending ramus and condylar process on the mandible. The genus Eothenomys could be classified into two subgenera. Moreover, it was inferred that the varieties of skulls characteristics in E. miletus, ...
Radek Aulicky1,*, Vaclav Stejskal1 and George Opit2
... of increasing the human safety. Therefore, we determined efficacy of 1-, 6-, and 24-h DDVP (evaporation rate: 0.15 ± 0.02 g.day-1) strip exposures on 5 stored-product pests, namely, Tribolium castaneum, Oryzaephilus surinamensis, Cryptolestes ferrugineus, Rhyzopertha dominica and Sitophilus granarius in an experimental chamber. We tested two DDVP evaporation regimes from the strips, namely, “preventiv...
Ghulam Ali1,3, Wopke van der Werf2 and Just M. Vlak1,
...t survived exhibited the same food consumption and weight gain. This study did thus not reveal any sub-lethal effects of exposure to the virus on food consumption and weight gain. There was no viral dose dependency observed in food intake or weight gain by infected larvae, suggesting there is no increased crop damage upon virus treatment but rather a decrease to be expected.
...

Mujib ur Rahman1, Munir Khan2*, Himayatullah Khan2, Malik Muhammad Shafi2 and Haidar Ali2  

...business in district Charsadda of Peshawar Valley. A total of 51 respondents from the three villages namely Utmanzai, Rajjar and Matta-Mughal-Khel were randomly selected. A comparative analysis of chadar and suit cloth was done regarding sale in Charsadda market and other cities of the country. In Charsadda market, 3/4 producing units sold their products...

Nighat Seema1*, Muhammad Hamayun1, Husan Ara1 and Raham Sheer Khan

...d enhance the extent of usage of organic compounds by the plants available in the soil and hence lowered the contents. During drought stress, ammonium ion concentration was increased in all endophytic treated soil except that of A. niger. Similarly, inoculation of endophytic fungi did also increased the value of soil nitrate ions in the range of 26.86±1.34~39.39±1.97 mg kg-1 with or without drought stress. Inoculation of endophytic fungi increase...

Muhamad Jawad*, Shahid Riaz Malik, Muhammad Aqeel Sarwar, Muhammad Asadullah, Israr Hussain and Rabia Khalid 

... yielding. So, it is advisable to use genotypes in the ideotype breeding program to enhance the desirable variation.  

...

Sahar Shibli1,2*, Farzana Siddique1, Saeeda Raza2, Zaheer Ahsan3 and Irum Raza4 

...luation of peanut butter samples showed overall good acceptability of product among the people. Storage study of peanut butter samples demonstrated shelf stability of product up to three months at room temperature. 

...
Asim Faraz1,*, Muhammad Younas2, Abdul Waheed1, Muhammad Yaqoob2 and Kashif Ishaq3

 

...jand, khagal, dhaman, persain, khawi, bui, bhakra, kari, laana, phog, karir and khar laana. Regarding hair mineral status Ca, Mg, Cu, Zn, Fe and Mn concentrations were found to be significantly different (P<0.05) between calf groups being higher in IMS as (685.07±15.86, 595.67±15.86; 104.33±5.12, 101.17±5.12; 7.08±0.34, 6.73±0.34; 65.33±2.25, 59.33±2.25; 322.20±8.67, 311.10±8.67; 46.53&pl...
A.H. Shahzad1, A. Sattar1,*, A. Husnain1, I. Ahmad2, N. Ahmad1, D. Nak3 and Y. Nak3
Mahboob Ali1, Muhammad Avais1, Muhammad Ijaz1, Memoona Chaudhary2, Riaz Hussain3, Amjad Islam Aqib1, Naimat Ullah Khan4, Muhammad Luqman Sohail5,*, Madiha Khan6, Mumtaz Ali Khan1, Mehtab Ahmad7, Muhammad Sajid Hasni2Imran Qaiser8, Ghazanfar Rashid1, Ikramul Haq1 and Ikramullah Khan8
... from 53.03% of the milk samples, with significantly higher (P<0.05) prevalence in Cholistan desert (66.10%) as compared to Suleiman range (57.98%). Blind quarter prevalence of S. aureus subclinical mastitis was 8.46% and 11.59% (P<0.05) in Cholistan desert and Suleiman range, respectively. The non-parametric statistical analysis showed significant association (P<0.05) of thin body condition, older age, lack of teat dipping...
Menderes Cenet
...is of eight floral honey samples from Kahramanmaraş region were analyzed and seven samples were the unifloral and the one was the multifloral origin. The microscopical analyses indicated that 61 taxa (41 at the genus level and 20 at the species level) belonging to 34 families were identified. Astragalus sp., Trifolium sp., Myrtus communis and Castanea sativa we...
Muhammad Furqan1, Usman Ali1,*, Anjum Ara1, Muhammad Mudassar Shahzad2, Muhammad Siddique Awan3, Riaz Aziz Minhas3, Khawaja Basharat Ahmad3, Qamar Zaman Qamar4 and Naeem Iftikhar Dar5
...) 18 localities were sampled from 3 zones created in Mirpur district (Azad Jammu and Kashmir). Random transects of fixed length were laid and number of active burrows counted and habitat analysed using quadrat method. Mongoose was distributed with average population density of 9.8 animal/km2, with highest (17.8 animal/Km2) density at Kalyal and lowest (3.5 animal/km2) at Panyam. Most favored altitudinal range was 450 m to-5...

Babar Tasim1, Tariq Masood1*, Zafar Ali Shah1, Muhammad Arif2, Ata Ullah1, Ghazal Miraj3 and Muhammad Samiullah4 

...r to compare the biochar samples obtained on the base of its physical and chemical properties. Physico-chemical characteristic such as pH, EC, proximate composition, macro and micronutrients, surface functional groups identification by Fourier Transform Infrared spectroscopy (FTIR) and structure morphology by Scanning Electron Microscope (SEM) were carried out in biochar samples by using standard methods. Statistical analysi...

Masaud Khan1*, Muhammad Jamal Khan1, Tahir Sarwar1 and Muhammad Jamal Khan2 

...asing Nitrogen doses and same pattern was observed with the decreasing irrigation levels and decreasing nitrogen doses. When irrigation levels were increased by 10 and 20% nitrate leaching increased by 38 and 48%. Similarly when Nitrogen levels were increased by 10 and 20% increase in nitrate leaching was about 20 and 35% but when irrigation level were decreased by 10 and 20 % nitrate leaching also decreased by 20 and 48% and when the nitrogen level was decrea...

Muhammad Ilyas*, Sardar Ali Khan, Shahid Iqbal Awan and Shafiq-Ur-Rehman 

...sults indicated that the said breeding material might be better suited for heterosis breeding under drought prone semi-arid areas. 

...

Salman Ali*, Inamullah, Muhammad Arif, Mehran Ali, Muhammad Owais Iqbal, Fazal Munsif and Arsalan Khan 

...yield (grains ear-1, thousand grains weight, grain yield and harvest index) components of maize. Increase in K levels beyond 75kg ha-1 showed a slight decrease in yield. It is concluded that water stress at each critical crop growth stage can drastically reduce crop yield, therefore, five times irrigation each at (mentioned) crop growth stage along with 75 kg ha-1 K is recommended for higher maize production. 

...

Zakiullah1, Muhammad Farid Khan1*, Muhammad Mohibullah3, Muhammad Iqbal2, Irfanullah3, Faheemullah3, Madiha Urooj1 and Uzma Arif1 

...arch Institute (CCRI) Pirsabak, Nowshehra (K.P.K) Pakistan during the year 2015. The results displayed highly significant (P≤0.01) variation among above six OPVS for days to germination, plant height, leaf area, number of grains ear-1, grains moisture % at harvest, grain yield and biological yield. Mean square values due to general combining ability were highly significant (P≤0.01) for all the experimental parameters except grain yield and biological yie...

Raza Ullah1* and Jamal Shah

...elected using multistage sampling technique and Analysis of Variance was used to compare the average wheat yield of farmers using different combinations of the two risk coping tools namely, off-farm diversification and precautionary savings. The results suggested that adoption of risk coping tools, particularly the simultaneous adoption of multiple tools at the same time, significantly red...

Muhammad Rameez Khan1, Muhammad Imtiaz2, Farhatullah3, Sohail Ahmad1 and Sajid Ali1* 

...Galaxy-2013, PS-2005, Faisalabad-2008, Shahkar and some local lines. Future wheat improvement programmes must be encouraged to consider resistance to powdery mildew, particularly for lines to be cultivated in the Himalayan region of Pakistan. 

...
Shamesa Maryam1, Ajaz Ahmad Sandhu1, Imran Bodlah2*, Muhammad Asif Aziz2, Ayesha Aihetasham3

 

... Pentalonia nigronervosa, Sitobion avenae, Greenidea (Trichosiphumpsidii and Cinara (Cupressobium) tujafilina have been recorded for the first time from this area. General characters, measurements, distribution and host plants have been given for all recorded species. The present study can be helpful for aphid management of different plants/crops in Gujranwala District.
...
Syeda Beenish Bukhari1, Sidra Khalid1*, Shahid Bashir1, Riffat Mehboob2, Humaira Waseem2
...ects and fear of lack of safety of vaccination procedure/ dirty needles. Analysis revealed a significant association between maternal education and barriers to complete vaccination course.
...

Shumaila Nazeer1, Iqbal Javed1*, Allah Bakhsh2, Mudassar Yasin2, Haroon Javaid3 and Kaniz Fatima1 

... by selecting convenient sampling technique. Along with export margin analysis of both markets, an effort is made to quantify impact of the major determinants on export of mandarin (Kinnow) to UAE and USA. According to data properties and suitability, multiple regression analysis technique is used for UAE and binary logistic regression technique was used for U
Muhammad Shabir Shaheen1, Shahid Mehmood1, Athar Mahmud1, Jibran Hussain1, Ahmed Sultan Jatoi2, Muhammad Yaqoob3, Sohail Ahmad1 and Arshad Javid4,*
... design and were fed the same quantity of feed. The birds were subjected to 4 heating systems during brooding (Floor Heating, Diesel, Gas and Electricity Bulb). Growth performances, blood glucose, cholesterol level and economic appraisal in term of running cost / Kg of live meat were recorded. The results revealed that all strains generally but Ross 308 particularly maintained on floor heating system (FH) exhibited significa...
Tamoor Azeem1,*, M. Yasin Tipu1, Asim Aslam1, Sajjad Ahmed2, Salman Ahmed Abid1, Abdullah Iqbal3, Naeem Akhtar4, Muhammad Saleem4, Aamerzish Mushtaq4 and Sajid Umar4
...g haemoparasitism. Blood samples were collected from 200 camels of either sex and different ages from district Attock. Samples were processed for haematobiochemical analysis and thin smear stained with Field stain according to standard procedure. Out of 200 camels 46% of them have blood parasites. Trypanosoma was the most prevalent (32.5%) followed by diptelonemiasis (8%). Babesia and Anaplasma was seen in 3% camels. Prevalence of diptelon...
Muhammad Tufail1, Naila Chand1, Rafiullah1, Shakoor Ahmad2, Rifat Ullah Khan2, Muhammad Mobashar3 and Shabana Naz4,*
...the effect of Mannanoligosaccharide (MOS) on the performance and gut morphology of broiler chicks (Ross 308) during the finisher phase. A total of 180 of 22 days broiler chicks were distributed into 3 treatments with 3 replicates having 20 chicks per replicate. One group was kept as control and the birds in this treatment were fed on basal ration without alteration in feed contents while the other treatments MOS-50 and MOS-1...

Khwaja Tariq Ziad1, Umar Hayat1, Muhammad2 and Muhammad Suleman Bacha3* 

...y channels and their transaction costs, the role of the mid-agent opportunistic behaviour in the relative milk supply chain. Using case study and qualitative method approach, eight key informants including five farmers, one local middleman, and representatives of two dairy companies were interviewed during the study, focusing the social aspects and people behaviour towards the dairy sector. Results showed a lack of coordination among small-scale farmers and as...

Shamsher Ali1*, Muhammad Jamal Khan2, Zahir Shah2, Naveedullah3 and Abdul Jalal1 

...align: justify;">Soluble salts and exchangeable sodium in soils are the main constraints in the agriculture ecosystem creating major threats to crop productivity and, hence, affecting socio economic condition of the farming community. Growing of salt tolerant crop genotypes of species on salt affected lands is an effective tool for getting desirable productivity. A two-factor experiment us...

Muhammad Amin1, Aftab Ahmad Khan2, Abida Perveen1, Zareen Rauf1, Sher Shah Hassan2*, Muhammad Arif Goheer2 and Muhammad Ijaz2 

...uo; or “crawling Disaster” as it is a plodding physical process and its effects spatially varies. Misperception on drought and its features is common in defining policies and caused delays in its prevention (Gore, 2002). Pakistan is one the counties which is affected badly by drought. In near Past, Pakistan faced one of the worst drought span drought in 50 years from 1998 to 2003 which harshly affected socially and economically. The present study w...
Rahat Afza, Muhammad Afzal, Muhammad Zeeshan Majeed and Muhammad Asam Riaz*
...nd Coccinella transversalis in the presence and absence of prey and the impact of sublethal concentrations of insecticides on intra-guild predation (IGP) and aphid consumption by coccinellids. Data revealed that in the absence of prey larvae of C. septempunctata exhibited more predation on C. transversalis while the predation of C. septempunctata on C. transversa...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
... of S. litura (Faisalabad, Chiniot and Sargodha), under laboratory conditions. Both NPV and Bt were applied using diet incorporation method. Two concentrations of a NPV based formulation (Somestar): 1x107 and 1x108 POB/ml, and three concentrations of a Bt based formulation (Dipel): viz., 0.1, 0.3 and 0.5 μg/g, were applied alone and in different combinations against 2nd and 4th i...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
.../i> and Streptococcus sanguis. Capsaicin extracted from chili peppers were selected to explore the antibacterial activity against the four cariogenic bacteria in terms of acid producing and biofilm. The antibacterial activity of capsaicin was evaluated by the minimal bactericidal concentration, change of pH in the first 12 h and OD values of biofilms. Besides, fluorescent images of ...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Nosheen Fatima1, Raheel Babar3 and Ikramul Haq4  

Aisha Siddiqua1*, Munir Ahmad1 and Nusrat Habib2 

...t this might be the necessary requirement to sustain wheat growth in Pakistan in future. Farmers are expected to receive significant positive benefits from the adaptation of the combination of strategies. Farmer’s adaptive capacity, farm size, access to credit and sources of information on climate change are significant drivers of adaptation to climate change. 

...

Syed Amjad Kamal Jan* and Naushad Khan 

...rough Yamani Formula 100 sample size was fixed and distribute the sample according to proportionate formula between two tehsil, Balambat 54 and Lalqila 46. Similarly, semi structured questionnaire was used for data collection while descriptive statistics and Cob Douglass Production Function were used for data analysis. The results indicate that all rice grower were literate while the secondary level was more than the other l...

Sonia1, Khuram Nawaz Sadozai1*, Noor Paio Khan2, Abbas Ullah Jan1 and Gulnaz Hameed3 

...p’s input and aforesaid climatic variables for the period of thirty years (1985-2015) was employed to figure-out the major findings. Econometric diagnostic tests were used to verify the model stability. Therefore, LM test was encompassed to detect the serial correlation. The result of Hausman test has suggested applying “Fixed Effect Model” for further analysis. Major estimates of the overall 30 years data reveal that among non-climatic varia...
Mehwish Akram1,2,* and Naeem Rashid1
...ons (LNv) towards the dorsal neurons (DN). Moreover, it was observed that lack of the potassium channels also caused a robust increase in sleep and reduction in the fly’s active period during the day. We observed that the normal circadian control of the morning and evening anticipation is also dependant on these potassium channels. The flies deficient in IRK1 channels didn’t show an evening anticipation peak. Another interesting disclosure during t...
Zhi YiJin1, Shao MingQin1,*, Cui Peng2 and Chen Bin1
...using instantaneous scan sampling in the Poyang Lake watershed in the Jiangxi province, China. The results showed that the Mandarin Ducks’ predominant behaviors were resting (36.56%), swimming (21.75%), foraging (20.88%), and maintenance (15.58%), indicating that during the winter, Mandarin Ducks spend most of their time accumulating energy. Mandarin Ducks reduced their energy consumption by increasing time spent on resting behavior. Females spent more t...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...tient and control plasma samples of ALL. Three highly reported reference genes namely RNU6-2, mir-16-5p and cel-mir-39-1 were selected as the candidate genes for normalization. Preliminary, quantification was performed through real time PCR in eight samples. The geNorm algorithm and comparative delta Cq method were used for selection of suitable reference gene out of the three candidate genes. The validation studies w...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1

Farhan, Shahid Ali* and Syed Attaullah Shah 

...-stage stratified random sampling technique. Primary data was collected through well designed interview schedule. Normalized restricted trans log profit function was first approximated for further estimation of output supply and input demand elasticities. Results indicated that response of output supply to the price of wheat and land was positive and elastic. Output supply in response to wage rate was negative and inelastic. Output elasticity in response to fe...
Dur-E-Shahwar1,2, Riaz Ahmad Sheikh1, Nazia Jamil2*
... water contaminated soil samples collected from three selected sites of Eastern Potwar, Punjab, Pakistan. Forty seven bacteria were isolated out of which five (F1, F3, F20, F23 and C16) were selected on the basis of high optical densities of 0.7, 70% oil and grease reduction potential and maximum CFU/ml of ≥3 ×106. The weights in g/l of biosurfactants produced from F1, F3, F20, F23 and C16 were 0.52, 0.93, 1.58, 0.52 and 1.56 respectively. ...

 Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2

...i>using Adult Motility Assay (AMA). Crude Ethanolic Extracts (CEE)of dried leaves of A. lebbeck L. and whole plant of C. sinensis L. were prepared by cold maceration method. The anthelmintic activity was analyzed by adult motility assay (AMA). Levamisole was used as positive control to compare the efficacy of CEE of both plants. Adult worms were treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of...

Rizwan Ullah Shah* and Iqbal Munir 

...nducted for optimizing basal medium through somatic embryogenesis (cotyledon and hypocotyl) for callus induction followed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l),...

Omer Baris Ince* 

...se. In this study, blood samples were collected from clinically healthy sheep and bovine in the surrounding region of the southeast of Konya province that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aimed to show the disease-related status of the selected region, to investigate the active virus circulation and determine the ...

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

...gainst Mungbean yellow mosaic virus (MYMV) disease. Exogenous use of Salicylic acid (SA) and Benzothiadiazole (BTH) as elicitors enhanced the resistance in mungbean plants by triggering the SA pathway. Induced resistance was assessed by evaluating the appearance of symptoms and detection of virus titter through ELI

Naseer Ahmed Abbasi1, Mian Noor Hussain Asghar Ali1*, Bilawal Abbasi2, Shakeel Ahmed Soomro1, Najeeb Ahmed Khan Nangraj1, Jam Ghulam Mustafa Sahto1 and Sajjad Ahmed Morio1 

... potential time and cost saving methods can assess the suitability of land. Finding shows that the fuzzy set method is different from parametric method. The influence of explicit weight on land characteristics is combined with the evaluation of land characteristics. The correlation coefficient is high for both indices, but results obtained with fuzzy set method (r=0.97) showed a higher accuracy with yields than those obtained with the parametric method (r=0.86...

Hina Ali*, Iram Shaheen and Feroza Hamid Wattoo 

...alysis of drinking water samples of different areas of Rawalpindi. The water samples were collected into sterile containers from the different localities of Rawalpindi such as Dhokekala khan, Morgah, Scheme-III, Gulshandadan khan, Satellite town, Dhoke kashmirian, Pandora, Bahria town, University campus (Mehr water) and Air Port. The pH of PMAS-AAUR campus water ( Mehr Water) was found within WHO recommended values (pH limit...
Jam Nazeer Ahmad1,2,*, Muhammad Jafir1, Muhammad Wajid Javed1, Sumaira Maqsood3 and Samina J.N. Ahmad1,2,*

Muhammad Ali1,2*, Norsida Man2 and Farrah Melissa Muharam2 

...rough multistage cluster sampling technique. In order to measure motivation of respondents, likert scale items comprising 1 as strongly disagree to 5 as strongly agree were used. The research findings reveal that farmers were motivated to manage their agricultural risks and overall their motivation level was high. Nevertheless, still efforts are required by all stakeholders to keep the motivation level high of farming commun...

Rehmat Ullah* and Khalid Nawab 

...a Ismail Khan, Swat, Charsadda and Mansehra) of Khyber Pakhtunkhwa selected through multi-stage sampling technique. Furthermore, four union council viz. Band Kurai, Baidara, Khanmai and Baffa from the sampled districts were selected. A total of 384 respondents were selected through unknown population sampling formulae i.e. 96 from each Union Council. Dat...

Shakeel Ahmad Anjum1, Muhammad Mohsin Raza2, Sami Ullah1, 3*, Malik Muhammad Yousaf2, Ahmad Mujtaba1, Mumtaz Hussain2, Muhammad Jahangir Shah2, Bashir Ahmad2 and Ijaz Ahmad4 

...ersity of Agriculture Faisalabad, Pakistan in a randomized complete block design (RCBD) with four replications. Net plot size was maintained 7.0 m × 3.6 m. Different tillage practices, i.e., deep tillage, conventional tillage, minimum tillage, simple cultivation flat sowing, simple cultivation ridge sowing, zero tillage dibbling and zero tillage drill were performed as the experimental treatments. Results showed that maize hybrid sown under deep tillage ...

Rafiullah1*, Abdur Rahman2, Khalid Khan1, Anwar Ali1, Arifullah Khan2, Abdul Sajid3 and Naimatullah Khan3 

...selected. A total of 800 samples were collected from different areas of District Lakki Marwat and District Peshawar. All the samples were processed through different diagnostic techniques, including Microscopy, ELISA and Real-time PCR for diagnosis of Theileria parva at Veterinary Research Institute Peshawar. The overall prevalence for Theileriosis was 2...
Liqin Liu, Maoting Wang, Zhenming Lü, Li Gong*, ChangWen Wu, Baoying Guo and Lihua Jiang
...eveloped twenty-one microsatellite loci for S. pharaonis through next-generation sequencing technology. A total of 100 alleles were detected, and the number of alleles per loci ranged from 2 to 9. The observed and expected heterozygosities per loci ranged from 0.000 to 0.531 and from 0.031 to 0.751, respectively. Polymorphism information content (PIC) showed that six loci were highly informative (PIC > 0.5). Five loci (CL1142, CL1770, CL2683, CL3494,...

Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1 

...rulence enhanced upon passaging in chickens causing clinical signs and deaths. In the present study brain samples were collected from twelve pigeon flocks exhibiting signs of nervousness and diarrhea in a live bird market (LVM) in Egypt. All collected samples were positive for NDV and negative for avian influenza virus and infectious bronchitis virus. Seven positive <...
Nasira Khatoon1, Aly Khan2,*, S.S. Shahid3, Z.S. Saify4, M. Arshad Azmi1 and Wali Khan5
Muhammad Usman Asif*, Raza Muhammad, Waseem Akbar, Mubasshir Sohail and Muhammad Awais  
...worms. Moreover, the perusal of data showed that the population trend of all insects remained below economic threshold level (ETL) during the period of study.  
...
Wen Chao Liu1,2, Kwan Sik Yun3 and In Ho Kim2,*
...he relative weight of bursa of Fabricius was increased by supplementation with 1,3-DAG (quadratic, P<0.05). In conclusion, dietary supplementation of different levels of 1,3-DAG in broilers diets could improve the growth performance, modify blood lipid profile and promote the development of bursa of Fabricius. 
...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
... healthy animals. At the same time, significant decrease was observed in RBCs (red blood cells), Hb (haemoglobin), and HCT (hematocrit) in Fasciola infected animals compared to health animals but no significant difference was observed in MCHC (meancorpuscular hemoglobin concentration) quantity within both animal categories.
...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...tudy was conducted in Faisalabad, Pakistan from June 2016 to January 2017. A total of 152 clinical samples were processed according to standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Carbapenem-resistant isolates were subjected to detection of Metallo-beta-lactamase production by Double D...
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...cts /allelochemicals and salicylic acid on the growth of Penicillium digitatum causes citrus green mould by using 7 plant extracts alone and in combination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by Chabir (33.60%) and Garlic (29.16%), while minimum growth inhibiti...
Ayesha Chaudhary1, Fouzia Mumtaz1, Muhammad Yaseen2* and Muhammad Younis Afzal3 
 
...se spread, and careless usage of natural resources. Due to these effects, pastoral families are at high risk, hence there is a need to make farmers aware of the reasons and causes of climate change so that they may contribute to their side in actively advocating the cause. The present study was survey-based research and carried out in the Faisalabad district of Punjab, Pakistan. The data was collected using a multistage
Shahid Yousaf1*, Salman Khurshid1, Saqib Arif1, Hafiza Mehwish Iqbal1, Qurrat ul Ain Akbar1, Najmus Sahar1 and Ghulam Rasul2
...ndard techniques. Thirty samples of wheat (including 11 varieties and 19 composite samples) were analyzed. Samples were widely varied in test weight (72.4-79.9 kg/hL) and wet gluten contents (15.4-31.5%) but showed narrow ranged moisture contents (10.8-13.3%). The NIR values were significantly related to the referenced values for test weight, wet gluten content and moisture with the correlation coefficients of 0.96, 0.83 and...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
Sakhra Mahmood1, M. Younus1, A. Aslam1, A.A. Anjum2, S. Umar3, Aamerzish Mushtaq3 and M.L. Sohail4,*
...one group was fed with basal diet (control group) and other was fed with AFB1 diet without any detoxifying agent. Growth parameters (body weight gain, feed intake, and feed conversion ratio), hematology (hemoglobin, haematocrit, erythrocyte sedimentation rate, and total leukocyte count) and immune response were recorded and analyzed by duncan test for 6 weeks. Results showed significant reduction in the deleterious effects of AFB1 in each of the tested paramet...
Osman Ergene1,*, Isfendiyar Darbaz1, Serkan Sayiner2 and Selim Aslan1
...he study, blood and milk samples were taken from fifty-eight cows at 28, 30, 32 and 40 days after artificial insemination (AI). Transrectal ultrasonography (TRUS) was performed on all cows on days 30 and 40 after AI. Measurements were carried out on 9 cows whose pregnancy was confirmed and 9 cows whose embryonic mortality (EM) was determined. In PAG-Serum, there was a statistically significant difference (P <0.05, P <0.01) between the measurements on 40t...

Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*  

...xtracts as well as crude saponins isolated from leaves and roots of Sarcococca saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against P. fluorescens and A....
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...%)] are optimized at the same time and the appropriate value ranges for the input factors [temperature (oC), humidity (%) and feed per hen (g)] that provide effectiveness ii) Determination of targeted improvement values for the ineffective months to become effective. With this hybrid approach, which is based on the integration of the Data Envelopment Analysis (DEA) and Grey Relational Analysis (GRA), the effective months are determined by DEA method...
Liyan Zhang1,2, Zhidong Zhou1,3, Haiping Li1,2, Yanlong Qiao4, 5 and Yueping Zhang1,3,*
...tic diversity across the sampled geographical range of the species. However, no phylogenetic structure corresponding to geographic location was observed from the neighbor-joining (NJ) tree and haplotype network analyses, and gene flow and exact P test results revealed that a wide range of gene flow occurred among the four S. japonicus populations. Analysis of molecular variance (AMOVA) and Fst analysis showed that genetic variat...
Doğukan Ölmez1 and Gonca Alak2,*
...For this purpose, hydrolysates were prepared by enzymatic methods at different time periods using different trout byproducts. In this study, two different groups (viscera and muscles) of trout processing wastes were hydrolyzed by enzymatic method based on specific time periods (0, 30, 60, 90, 120 and 180 min) for this purpose. Protein profiles, molecular size and distribution changes of the obtained hydrolysates were calcula...
Yang Liu1, Jing-xin Mao2, Xiao-dong Wei2, Man Yi2, Xiao-long Zhang2, Ke Zheng3, Xian-xin Chen4, Guo-Ze Wang5 and Bing-bo Chen1,*
...rmining the efficacy and safety value of biotechnology produced fulvic acid (BFA) as a functional feed additive. Four BFA products produced by lactobacillus, bacillus, yeast and fermented BFA from above 3 mixed bacteria were added by a proportion of 1.5% to the conventional feed. Then the Sprague-Dawley rats were fed by normal feed and 4 different BFA feed for 4 weeks. It was observed that the counts of neutrophils, white blood cells, and monocytes was signifi...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...sed to develop a new and safe strategy for controlling ticks, so as to produce dairy and meat products with minimal residual contamination.
...
Iqra Bano1,*, Moolchand Malhi2, Pershotam Khatri3, Saeed Ahmed Soomro2, Hira Sajjad4, Ambreen Leghari5, Muhammad Awais2, Safia Kandhro6 and Shakeel Ahmed Lakho5 and Munaza Soomro7
...) (n=6), respectively. Basal diet given to the animals consist of roughage and concentrate at the ratio of 65:35. In group C, the goats received only basal diet while in group SY the goats consumed basal diet along with SY 0.3 mg/kg.BW for 8 weeks. The results revealed that testicular weight, thickness, and circumference increased in SY as compared to C. The body weight of goats was also i...

Muhammad Zulfiqar1*, Irshad Abbasi2, Himayatullah Khan1, Arjumand Nizami3, Abdul Hakeem4, Jawad Ali3 and Muhammad Jamal Khan

Ayesha Khan*, Zubair Ahmad Khan, Urooba Pervaiz And Mehmood Iqbal 

...ere interviewed making a sample of 81 extension agents; 10 agricultural officers and 71 field assistants. The study results indicate that 77% field assistants were educated up to Matric with two years diploma while 10% agricultural officers had M.Sc. (Hons) degree with the majority of extension agents having job experience of more than 20 years. The main sources of information for the majority (79%) of extension agents were extension publications and training....
Catherine J. Allen
...ing (Quechua sumak kawsay, Aymara suma qamaña, Spanish buen vivir, vivir bien) have entered the political discourse of the Andean nations (Bolivia, Ecuador, Peru), serving as rallying points for networks of indigenous federations, environmentalists, NGOs and intellectuals united in a critique of neoliberal development paradigms. Ecuador and Bolivia have enshrined sumak kawsay and su...
Temel Göktürk1,* and Göksel Tozlu2
...arvae of the common pine sawfly (Diprion pini L.) and the European pine sawfly (Neodiprion sertifer) can cause epidemic, around the globe. Recently, an outbreak has been reported in young Scots pine (Pinus sylvestris L.) forest in Artvin - Borçka, Turkey. In order to propose effective control measures, this study was conducted to estimate the effectiveness of two biopesticides against D. pini <...
Naveed Ahmad1,*, P.J.A. Siddiqui1, Amjad Ali1, Khan Mir Khan2, Rafaqat Masroor3, Noor ul Akbar4, Muhammad Amin5 and Mohammad Attaullah6
...e fed to visual apparent satiation two times a day for a period of eight weeks. At the end of the trial, P40 appeared to have significant (P<0.05) influence on the growth performance. The percent weight gain, average daily weight gain and specific growth rate (SGR) increased significantly by increasing dietary protein level from P30 to P40 (P < 0.05), and further increases in protein level (P45, P50) reduced growth. The best feed con...

Dilshad Ahmad1* and Muhammad Afzal2

...d multiple stages random sampling technique used for data collection of 400 Bt and Non-Bt cotton farmers for the year of 2012-13. The results of the study have indicated Bt cotton farmers of both districts with higher technical efficiency score as more efficient than Non-Bt cotton farmer. Empirical findings specified a cropped area, seed, labor, fertilizer and irrigation were the significant variable of increasing cotton production of Bt farmers while pesticid...

John Ogunsola1*, Richard E. Antia2, Benjamin O. Emikpe2 and Olajumoke A. Morenikeji

...both sexes placed in the same enclosure with some degree of access to one another. Among mammals, survival rates of neonates ranged from 0% in Dorcas gazelles (Gazella dorcas; n=2) to 100% in Green monkeys (Chlorocebus sabaeus; n=2) and Patas monkeys (Erythrocebus patas; n=3), and crocodiles (Crocodylus niloticus; n=32). Muscovy ducks (Cairina moschata), black cobra (Naja nigricollis) and lions (Panthera leo) had survival ra...

Irfan Ullah*, Abbas Ullah Jan, Muhammad Fayaz, Amjad Ali and Atta Ullah Shah 

...or analysis. The uncompensated own price elasticity for milk, fruits, and baked products is greater than unity which suggests that these food groups are highly responsive to their own price change. Prices of these food products should be kept constant. Imposition of any sale tax could create a huge loss in consumption for these commodities. Milk, meat, fruits, other cereals, tea and coffee and baked products were treated as ...
Alexus McLeod
...odern Maya conception of sacrifice as a virtue and a key aspect of well-being. To do this, I look to premodern Maya sources—particularly, Classic Period texts and images (in Part One) and the Postcontact text Popol Vuh (in Part Two). I offer interpretive reconstructions, to illustrate key features of the Maya concept of sacrifice as a principle of growth as well as metaphysical and ethical renewal, as demonstrat...
Seth Ligo
...nd Hinduism’s most sacred pilgrimage destination, contemporary social networks are interwoven with historical and religious narrative. In fact, in this densely populated and ancient space it is imperative not only to know one’s extended family, one’s community, and one’s history, but also the history of the city more broadly, the stories that are mapped onto it, and all the neighbors — both visible and invisible — one might ...
Christine Jeske
...dictory moral narratives saying that they should “help themselves” and also “wait on God and not take shortcuts.” I argue that churches are useful sites for attaining well-being in South Africa not only because they offer instrumentalist tools for acquiring resources and social networks, but also because churches offer malleability of personal narratives, which allows people to negotiate society’s conflicting narratives of moral w...

 Jennifer Mei Sze Ang

...-being focused on desire/satisfaction/positive emotions and the eudaimonic strands of well-being studies emphasizing autonomy. It shows that the strand of well-being which places good mood and satisfaction as central goals of a happy life are projects of bad faith, and that authenticity promotes a more fundamental notion of freedom than the conceptualization of a meaningful life based on autonomy. Finally, it argues that exi...

 Sasha S. Euler

... may be, it involves the same basic psychological needs beyond how people may choose to report or express resulting emotions. In particular, the theory of happiness proposed by the Hellenistic philosopherEpicurus provides broader, more inclusive categories and concepts which can be used to explain and possibly harmonize assumptions from particular traditions.

...

Shafique Ahmed Memon1,6, Arif Ali1*, Mehar-un-Nisa Narejo2, Ghulam Khaliq3, Imran Ali Rajput4, Muhammad Adeel1, Khalil Ahmed Memon5, Dzolkhifli Omar6, Muhammad Rashid7 and Abdul Rasool

...a nipponensis, Plesiochrysa ramburi and Apertochrysa sp. were provided with three different prey species, Aphis craccivora, Rhopalosiphum maidis and Corcyra cephalonica under the laboratory conditions (25±2 °C). The results indicated that a significantly increased pre-imaginal (19.93±0.24) and mean average days (70.33±0.55) of C. nipponensis on R. Maidis and also a significantly increased pre-imagina...

Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2 

...of M. phaseolina in soil samples collected during field survey at different districts of Sindh during 2015 and 2016 growing year. In soil sample maximum 41.371 cfu per gram of M. phaseolina with range of 27.00-48.40 were counted from Badin during 2016 followed by 40.2 with range of 27.00-45.20 during 2015 growing season. The plant tissues were carrying more M. phaseolina cfu as compared to soil sa

 Muqader Shah1,*, Hafsa Zaneb2, Saima Masood2, Rifat Ullah Khan1, Salahud Din1, Shakirullah3, Imad Khan2, Ambrina Tariq4 and Habib ur Rehman5

... in T6. However, tibiotarsal index decreased significantly (P<0.01) in T5. In conclusion, Zn and probiotic ameliorated the negative effect of HS. However, the bone parameters were not greatly affected by HS and the supplements.

...
Shehzad Ghayyur1,3, Sadia Tabassum1, Munawar Saleem Ahmad2Naveed Akhtar1 and Muhammad Fiaz Khan1,*
...onents of Oreochromis mossambicus. RBCs, Hb and Hct decrease significantly (p < 0.05) indicating an anemic condition, while the WBCs and platelet counts increased significantly (p < 0.05) in all the treated groups compared to the control group. Among the seral biochemical parameters, the level of blood glucose, cortisol and cholesterol were significantly increased (p < 0.05), which is an adaptive response to the stressor agent, while total plasma prot...

Arjumand Nizami1, Jawad Ali1 and Muhammad Zulfiqar2* 

...area pertaining to the Disaster Risk Reduction under overall domain of adaptation to climate change. Understanding occurrence and impacts of hydro-meteorological disasters on people’s well-being and which livelihood assets are significant for identifying coping strategies to deal with disasters are important ingredients to increased resilience of communities living in di
Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
...vae. Two laboratory bioassays, leaf dip bioassay (LDB) and topical bioassay (TB) were developed to check the efficacy of tested chemicals and essential oils. As seen from results, abamectin showed significant mortality (63.5%) of CLM larvae when topical bioassay was performed. Similarly, the percent mortality of CLM larvae was 53.8% after application of ...

 Abdul Khaliq1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Akhlaq Ahmad2 and Yasir Iftikhar3

... while, at 400ppm showed same resistance level in all. At 500ppm, lesser grain borer (LGB) was more susceptible as compared to others as in rice weevil. Nicotiana tabacum (L.) and Calotropis procera (Aiton) oil both at 15% were most lethal to LGB, weevil and red flour beetle after 72hours following by effect of Datura stramonium (L.) and Eucalyptus camaldulensis (Dehnh) with respect to control. Similar response was noted at 10% conc...

Saeed Ullah1, Muhammad Israr1*, Shakeel Ahmad2, Nafees Ahmad3 and Asif Yaseen

...D) is an expanding universal problem influenced all areas of human prosperity. This research contributes to this end by analysing the perceptions of farmer and its socio-economic influences towards LD in four purposively selected union councils i.e. Toru, Ghari Kapoora, Lund Khwar, Katlang-1 of district Mardan. A total of 90 farmers were selected through proportionate simple random sampling from 857 register farmers. Primary...

Saqib Bashir* and Bashir Ahmad 

...urity, plant height, thousand grains weight, biological yield and grain yield of wheat. The integration of pigeonpea green manuring with nitrogen levels significantly increased wheat growth, yield and yield components. In case of green manuring, plots in which pigeon pea crop was incorporated at the age of 90 days showed maximum plant height (103.3 cm), thousand grains weight (45.2 g), biological yield (10199 kg ha-1) and gr...

Manzoor Hussain1, Muhammad Awais2, Farhatullah3, Saiqa Bibi1, Muhammad Rameez Khan3, Afifa Khalid1 and Sajid Ali3* 

 Shaher Bano1, Nadia Naseem2 and Sarah Ghafoor1,*

...tructure and function of salivary glands. The localization of TGF-α in post-natal submandibular gland (SMG) of rats and humans is well-studied.However, less is known regarding post-natal sublingual salivary gland (SLG) development and expression of TGF-α. The comparison of TGF-α expression in SLG with that of its expression in SMG during post-natal rat salivary gland deve...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...ally for TNBC. The blood samples were collected from 37 TNBC diagnosed patients and 34 age-matched non-TNBC controls to investigate the expression of miRNA panel. Plasma was isolated by standard protocol of centrifugation and then isolation of total RNA including miRNA was done. About 100ng of isolated miRNA was converted to cDNA after addition of poly (A) tailing and then expression profiling was done by using real time-qPCR technique in which miR-specific DN...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

...istance of one of the causative agent of bovine mastitis, Staphylococcus aureus. Ninety milk samples were collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous exposure of mastitis. Initially surf field mastitis test (S...
Saleha Gul1,2, Muhammad Khisroon2, Ajmal Khan2, Attaullah1, Saira Gul3 and Gul Nabi Khan1,4,*
...ic hydrocarbons and nitrosamines cause the production of DNA adducts and leading to lung cancer. However, the adverse effects of cigarette smoke on other tissues are largely unknown. We explored the genotoxic effects of tobacco smoke on peripheral lymphocytes of active and passive smokers. Blood samples were isolated from 100 males’ including 25 active-, 25 passive- and 50 non-smokers. Alkaline comet as

 Sohaib Afzaal1, Usman Hameed2, Nasir Ahmad3,*, Naeem Rashid3 and Muhammad Saleem Haider1

...is of stress tolerance assays. Identification of the screened strains was further confirmed by 16S ribosomal DNA sequences and strain level differentiation was carried out by sugar fermentation tests. These strains were further analyzed for their ability to produce lactic acid. Overall, Leuconostoc mesenteroides was found as pre-dominant group (41.1% prevalence) among LAB strains in the isolated food samples. Some opp...

 Şaban Çelebi

...rol group received the basal diet (T-1), treatment groups were fed on the the basal diet plus the three experimental diets included one of the followings: 125 mg/kg vitamin E + basal diet (T-2); 0.5 mg/kg Selenium + basal diet (T-3) and 125 mg/kg vitamin E plus 0.5 mg/kg selenium + basal diet (T-4), respectively. Exper...

Huma Khalil1*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Muhammad Sajjad Khalil1, Farghama Khalil2 and Hafiz Khurram Shurjeel1 

Iram Maqsood1,2,3 and Ke Rong *1,2
...s. Consequently, for the sake of A. sinensis conservation, China took steps with the top priority of habitat restoration, controlled management in captive breeding centers, as well as initiation of reintroduction into the wild. Captive breeding centers focused on livestock, controlled sex ratio, promoting growth characteristics, population genetics, and organ preservation. In addition to this, the habitat restoration program committed to improving the q...

Bata Shalangwa Ishaku1, Batim Turdam1, Maimadu Abdullahi1, Waziri Ibrahim Anjili2 and Mayowa Olabode2 

...Nigeria. Fecal and blood samples were collected from 300 donkeys and analyzed using a standard laboratory procedure. Coprological examination conducted using flotation and sedimentation techniques showed that 276 (92.0%) were positive for gastrointestinal nematodes. A greater proportion (70.7%) of the nematodes were Strongyles followed by mixed infection of Strongyles and Parasacaris equorum (15.2%) and Strongyloides westeri...

 Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3

...ted to four dietary bioassay treatments and fed mash red and white sorghum grain with or without phytase enzyme (500 FTU/kg) for 12 days (including 4 days adaptation period). Standard lab protocols were adapted to measure proximate analysis, minerals and phytate content in grain and fecal samples. Sorghum cultivars were different in nutrient profile with red higher in protein content (11.41%). It was observed that phytase in...
Muhammad Zain Saleem1,*, Raheela Akhtar1, Asim Aslam1, Muhammad Imran Rashid2, Zafar Iqbal Chaudhry1, Muhammad Adeel Manzoor1, Bilal Ahmed Shah3, Rais Ahmed4 and Muhammad Yasin5
...ut B. abortus (causative agent of bovine) may cross the species barrier to infect the small ruminants which may complicate the control measures of brucellosis because most of the control programs rely on serological screening of brucellosis rather than molecular assay which could confirm the particular species circulating in ruminants. In this study 960 and 471 serum samples of goat...
Guo-hai Wang1, Zai-xi Yang1, Pan Chen1, Wei-ning Tan2 and Chang-hu Lu1,*
...ersers.

...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
... RAPD markers. The blood samples of purebred animals of eight exotic breeds (Thoroughbred, Arab, Anglo-Arab, Suffolk, Percheron, Clevland, Noriker and Hanovarian) present in Pakistan were collected from Army Remount Stables while those of four Pakistani indigenous breeds (Morna, Saen, Kakka Bralanwala and Anmol) from local horse breeders of Punjab province. Genomic DNA was extracted from the blood samples and amplified by PC...

 Mahroze Fatima1*, Muhammad Afzal2 and Syed Zakir Hussain Shah3

...y feeding high vitamin dosages. Supplementation of vitamin E decreased the saturated fatty acids (SFA) and monoenes while increased the poly unsaturated fatty acids (PUFA) compared to control group. Similarly, eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), docosahexanoic a...
Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3
...altose, mannose, xylose, sacchorose, sorbitol, mannitol, Arabinose and polysaccharides like gelatin and starch. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates belong to the same specie Anoxybacillus rupiensis with 99% similarity under Accession number KF254911 (JF82) and KC849452 (JF83). The DNA G + C contents were 56.854% and 56.754% for A. ru...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
...ted from blood and urine samples were identified through API® 20E kit. Antibiogram was determined by disc diffusion method and isolates were screened for ESBL production using double-disc synergy test (DDST). Molecular detection for the presence of bla-SHV and bla-TEM was carried out by polymerase chain reaction (PCR). Of 392 blood and urine samples, 120 were found positive for microbial growth. ...

Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1* 

... and haemagglutination assay (HA). Our results revealed that pH of the manure from time of its mixing with the NDV infected allantoic fluid till day 4 was 7, then pH values changed progressively and recorded 8.5 on day 12. After that, pH increased towards the alkaline side and recorded pH = 9 till day 33 of the experiment. Regarding moisture %, after mixing the manure with allantoic fluid the moisture % of the mixture was 67%, and this wet manure remained mois...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
..., including Anas formosa, Anas crecca, Anas strepera, Mergus squamatus, Accipiter nisus, Buteo hemilasius, Buteo lagopus, Passer montanus, Psittacula roseata and Emberiza elegans, were selected.The sequences of the GTPase effector domain (GED) of the Mx gene were determined by PCR sequencing. The haplotypes were analysed by DNA SP software. The Datamonkey Adaptive Evolution Server was...
Ashfaque Ahmed Nahiyoon1,*, Shahina Fayyaz2 and Nasira Kazi2
...harvesting and soil root samples were collected from different fields in the cotton producing regions of five districts of Sindh viz., Sanghar, Mirpurkhas, Umerkot, Mityari and Tando Allahyar. The analysis of samples resulted in the identification of one new species of soil nematode viz., Acrobeloides gossypii n. sp., along with three new record species viz., Tylenchorhynchus ewingi Hopper,...

Saud Khan and Inamullah* 

...apitulum-1 (974) and thousand achenes weights (51.4 g) were produced with MB plough which were statistically at par with cultivator, while higher achene yield (2080 kg ha-1) and oil yield (873 kg ha-1) were observed under MB plough tilled experimental units which were statistically at par with rotavator. In case of P levels, higher plant height (157.3 cm), higher number of achenes capitulum-1 (1028), thousand achenes weight ...

Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1* 

...cally through haphazard usage of medications and or biologically through massive use of homologous/heterologous vaccines especially the live ones. The aim of the current work is to estimate the persistence time for velogenic Newcastle disease virus (NDV) of genotype VII in poultry manure in compare to LaSota vaccine strain as a model of genotype II. Velogenic NDV of genotype VII are a major threat to the Egyptian poultry industry since 2011 onwards with contin...
Fan Yi1,2, Xiaobin Yang1, Shigen Ye1, Hua Li1 and Ruijun Li1,*
...r experimental groups: basal diet feeding group, 0.5% HCT continuous feeding group, 1% HCT feeding group, and 1% HCT interval feeding group. Three replicates were set for each treatment, and 25 fish were stocked on each replicate. The results of continuous feeding experiments showed that the continuous addition of 0.5% and 1% HCT feed for 28 days could very markedly increase peripheral leukocytes count, serum lysozyme activity, phagocytic rate, phagocytic inde...
Fakhra Soomro1,*, Riffat Sultana2 and Muhammad Saeed Wagan2
...Fieber viz. S. savignyi Saussure and S. rubescens rubescens (Walker) were studied exclusively form Sindh during the 2014-2016. Both species passed through six instars larvae. Emergence of larval instars in both species was started from the month of July just after monsoon rainfall, whereas, S. savignyi immature emerges earlier than S. rubescens rubescens and increases their density rapidly ...

Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1 

...istic activity against Fusarium oxysporum f.sp. alium cepa. They all were found to inhibit fungus showing inhibition zone 4.09 -74.97%. Hence, on the basis of these studies, it can be concluded that these bacteria can be used as a biocontrol agent to manage other fungal diseases of agricultural crops also. 

...

Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3 

...Pakhtunkhwa. 120 quarter samples were collected from suspected buffaloes in selected areas of Peshawar, Charsadda, Mohmand Agency, and Dara Adam Khel. Initially, California Mastitis Test was performed for screening of positive samples. Afterward, the bacterial profile was confirmed through biochemical testing. The quarter wise prevalence of subclinical mastitis was 25%. Within this, contri...

Syed Atif Hasan Naqvi* 

...d to use environmentally safe approaches to overcome the loss of grain yield in rice due to this disease. Climate change has a serious effect on this disease because in current scenarios cropping pattern has been changed and the stakeholders has shifted to some other crops rather the practicing ones. Since, farming community has adopted rice crop in non-core areas of Indian Sub-continent where it was out of question before this. Climate change has a drastic ef...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...s break down complex polysaccharide polymers of plant tissues into simpler monomer like D-galacturonic acids. In this study, polygalacturonase from grape skin was purified by salting out with ammonium sulfate, gel filtration on Sephadex G-75 column and ion exchange on Q-Sepharose column chromatography. Polygalacturonase was recognised as a protein with 47kDa molecular weight by SDS-PAGE. The optimum pH of purified polygalact...

Anum Khursheed1, Malik Muhammad Shafi2 and Haidar Ali2* 

...t Muzaffarabad. From 111 sampled respondents primary data were collected through pre-tested questionnaire. Three villages namely; Kundipiran, Kariandarar and Kulpana from union council Kahori were purposively selected. The study indicated that the majority of the sampled respondents were female. To compare the size of livestock holding paired t-test was used. Productivity of livestock kept by sa

Arshad Farooq* and Muhammad Zafarullah Khan 

...echnology among the sample farmers of both districts was due to their interests in cane farming, sugar mills support, mono and poly agricultural practices, lack of agricultural extension activities etc. Training and awareness programs through various types of mass media should be launched for reducing the knowledge gap and to equip the cane farmers with latest technology. 

...

Aziz Ur Rahman* and Syed Mehar Ali Shah 

..., Swat, Manshera and Charsadda of the Khyber Pakhtunkhwa province of Pakistan during 2017. Pooled analysis of variance indicated significant (p≤0.01) differences among the environments, genotypes and GEI for spikelets panicle-1, grains panicle-1, 1000-grain weight and grain yield. Across four locations, AUP-30 showed the highest number of spikelets panicle-1 (242.5), grains panicle-1 (228.6) and maximum 1000-grain weight (29.0 g). Rice RILs AUP-3, AUP-30 an...

Hafiz Umar Farid1, Muhammad Zubair2, Zahid Mahmood Khan1, Aamir Shakoor1*, Behzad Mustafa1, Aftab Ahmad Khan3, Muhamad Naveed Anjum4, Ijaz Ahmad5 and Muhammad Mubeen

...p-scaling of these water saving techniques for the welfare of mankind. 

...
Bushra Siddique1,*, Muhammad Tariq1, Muhammad Naeem1 and Muhammad Ali2
...before Olfactometer bioassays. For D. rapae, mummified aphidswere collected from wheat crop. Naive females were subjected to olfactometer tests. Seven different combined treatments of semiochemicals and plant extract were applied on filter paper strips at 3% concentration. The filter paper strips were placed in arms of olfactometer. The control arms were treated with n-hexane. Data pertaining to preference of C. septempunctata and D. rapae...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
..., respectively. When the same doses were applied for 48 h, the living cell rates were 90%, 83%, 82%, 78%, 52%, and 47%, respectively. Following applications of 25, 50 and 100 μM TA to LNCaP cells for 24 h, the apoptotic values (0.60% for the control group) were found 2%, 4%, and 17%, whereas the values for 48 h were 10%, 10% and 64% (0.48% for the control group), respectively. TA induced apoptosis of LNCaP cells depending on the dose and duration of exposur...
...rrando, Lorenzo Gallus, Asad Ali Muhammad, Abdul Jabbar and Khalil-urRehman, 2018. Assessment of Optimum Salinity Level for Maximum Growth and Survival of Nile Tilapia, Oreochromis niloticus (Linnaeus 1758) . Pakistan J. Zool., vol. 50(2), pp 585-594.
 
(v) Abdul Malik, Ghulam Abbas,* Abdul Ghaffar, Sara Ferrando, Lorenzo Gallus and Syed Sajjad A. Sha...

Hamid Ali, Muhammad Arshad, Ibad Ullah Jan, Muhammad Zamin*, Junaid Khan, Ikram Ullah and Mushtaq Ali 

...berose (Polyanthas tuberosa), is an important ornamental and perfumery plant. The research was conducted at University of Swabi during summer 2018 to evaluate the performance of tuberose under various concentrations of gibberellic acid and micronutrients. Experiment was laid in Randomized Complete Block Design (RCBD) using two factors i.e. gibberellic acid and micronutrients with three replications. Both the factors gibberellic acid (GA3) and micro nutrients h...

Tariq Shah1*, Umar Hayat1, Muhammad Suleman Bacha2 and Muhammad3 

...s along with independent sample t-test and dummy variable regression were used for analysis. It is observed during the study that livestock extension department and private livestock doctors are the key sources where farmers get advice regarding livestock production. Majority of the respondents are quite satisfied regarding the effectiveness of these advices. Results further indicate that farmers visit or contact livestock e...

Muhammad Nasrullah1*, Liu Chang1, Khurram Nawaz Saddozai2, Asaad Osman Khalid1, Rachel Bayisenge1 and Gulnaz Hameed3 

...lected through purposive sampling among the three Tehsil of district Mardan. A sample of 120 growers was selected and interviewed randomly through a prepared well- structure questioner. Different practices were grouped into five parts. Land rent of the respondent area was 61,750 rupees per hectare; nursery raising cost was estimated at 11,828 rupees per hectare. The bed preparation cost for tobacco is 12,300 rupees per hecta...

Shahid Ali1*, Asim Khan1, Aftab Khan1 and Bakhtawar Riaz

... proportional allocation sampling techniques. Data was collected by using a well-structured interview schedule. Cobb-Douglas production function was employed to assess technical efficiency. Maximum likelihood estimation techniques were employed for estimation. Results showed that all explanatory variables except pesticides have significant effect on tomato production. The results showed that a 1% increase in seed rate, labor, tractor hours, urea, DAP and irrig...
Abid Hussain1,2, Farida Ahmed3, Shyamali C. Dharmage4, Shafiq ur Rehman1, Muhammad Shahbaz Aslam5 and Zaigham Abbas1,*
... bronchial asthma at the same time were 3.9% prevalent. This study revealed a huge number of patients with diverse demographics, suffering from allergic diseases and sensitization to different allergens majorly aero allergens indicating poor indoor and outdoor air quality. This demands more public health facilities, and strict environmental regulations of air quality to manage allergic diseases in Pakistan.
...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
... this reason, 45 cloacal samples comprising from 23 Japanase quails and 22 chickens were analyzed by conventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipe...

Muhammad Ijaz, Sayed Rashad Ali Shah*, Muhammad Izhar-ul-Haq and Amir Afzal 

...ions and detached leaf assay against LLS pathogen. Most of genotypes during three years of study at early assessments under field conditions showed resistant response whereas, at late assessments most of genotypes were susceptible. In pathogen-host relationship several components of partial resistance have been proposed. Under detached leaf assay Virginia type groundnut showed minimum spots per leaf, sporulation, and product...

Fraza Ijaz1*, Umair Riaz2, Shazia Iqbal3, Qamar uz Zaman4, Muhammad Furqan Ijaz5, Hina Javed1, Muhammad Amjad Qureshi1, Zuhra Mazhar3, Ahmad Hassan Khan6, Hassan Mehmood7 and Ijaz Ahmad8 

...r of seeds per head, thousand seeds weight were recorded with Co-inoculation + Tryptamine @ 10-5 M (T8). Nutritious Parameters crude protein (30.23%), neutral detergent fiber (33.45%) and acid detergent fiber (26.56%) gave significant results as compared to control (T1). Results indicated that the combined application of Rhizobium species and Tryptamine performed better by improving growth and yield and quality parameters. It is concluded that precursor-inocul...

Muhammad Sohail1*, Asad Sultan2, Said Sajjad Ali Shah3, Muhammad Sajid1 and Adnan Khan4

... the collection of fecal samples and further analyses. Nutrient profile, digestibility coefficients, and apparent metabolizable energy were investigated. Fat percentage and metabolizable energy was higher in maize (4.1% and 17.04 MJ/kg). The protein levels in wheat varieties were 12.15 and 11.89% which were more than that of both of corn (8.21 and 8.05% respectively) and sorghum varieties (10.41% and 9.89% respectively). The bioavailability of crude protein wa...

Muhammad Usman Ghani1*, Muti Ur Rehaman Khan1, Asim Aslam1, Zubair Shabbir2, Li Bo3 and Naveed Anwar4 

...ific and sensitive PCR assay based on amplification of B2L gene was applied against conserved gene of Orf virus. Crusted scraps and biopsies from different portion of skin and blood were taken as sample for PCR. Gross, histopathological, hematological examination was carried out to rule out possible cause of infection and adopt preventive measure so that further losses may be avoided. 96 out of total were clinically positive...

Bina Khanzada1*, Ghulam Hussain Abro1, Tajwar Sultana Syed1 and Nazir Ahmed2 

...y, sugar, protein hydrolysate solution to enhance fecundity and fertility of the parasitoid under laboratory conditions and compared with the provision of flower nectors such as ornamental sunflower, merry gold and hollyhock in the laboratory. The ornamental plants sunflower, merry gold and hollyhock were also tested in the field for conservation of the C. flavipes. The results showed that during 2013 and 2014, the C. flavipes fed on Hollyhock produced more ca...
Muhammad Ahmad, Zohaib Noor*, Karim Johar Khan, Waqar Younas and Ajmal Hussain
...eeping other species the same as in group 1. At the end of experiment H. molitrix showed considerably high weight gain (P< 0.05) in group 2 as compare to column and bottom feeder fish species, conversely in group 1 higher weight gain was comparatively prominent in column and bottom while, C. catla showed low weight gain. Mean values of water quality parameters were conducive however temperature fluctuated between 26°C-29°C. Significant...
Faraz Akrim1,2, Tariq Mahmood1,*, Muhammad Sajid Nadeem3, Siddiqa Qasim1, Shaista Andleeb1 and Hira Fatima1
...recorded at 15 different sampling sites within the elevation range 699-1559 m above mean sea level (AMSL). The small Indian mongoose was found distributed at 30 different sites in the study area, having an elevation range 691-1624 m AMSL. The diet of Indian grey mongoose consisted of 16 prey species (15 wild and one domestic), and six plant species. The consumption of wild prey was 60%, while domestic prey contributed 19%, plants 14%, grits 2%, and anthropogen...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
...uth bass (Micropterus salmoides) reared in an in-pond raceway system (IPRS). Fish (initial average body weight: 35.68±2.12g) were randomly allotted to in-pond raceways (26.2m×5m×2.5m) stocked at two stocking densities (68 and 114 fish/m3, respectively). Fish were fed twice daily (08:00 and 17:00), and the daily ration feed was 4% of the body weight. No significant differences were observed in growth, digestive enzyme activi...

Khan Sher1*, Muhammad Nisar2 and Muhammad Subhan1 

...and sub clusters. On the same way lineage II split into two clusters at 19 % similarity index. The Genetic similarity (GS) based on SSR markers between pair of common bean lines showed high degree of dissimilarity in the genetic makeup ranging from 0.91 to 0.11. All the landraces of common bean collected from Malakand divisions KP, Pakistan, showed significant variations. 

...

Ayat Ullah* and Ayesha Khan 

... Using multistage random sampling technique data were collected from 395 respondents according to Yamane’s formula. The study uses the Kruskal–Wallis H test for checking differences among farmers knowledge with differences in their contact with extension service providers. The results revealed significant differences in farmers’ knowledge of chemical, biological and cultural control methods of pest with differences in the frequency of extensi...

Abdur Rehman1*, Iqbal Javed2, Zhang Nannan3, Muhammad Niamatullah1, Raheel Saqib4 and Allah Bakhsh

...t of agricultural modernisation and to provide the theoretical reference for the development of agricultural informatisation in Hebei province China. The relevant literature and the results of the research have been collected to understand the agricultural informatisation in Hebei province and adopted the system analysis method to focus on the existing problems and to put forward the signi...

Iftikhar Ud Din* and Qamruz Zaman 

... i.e. to find sufficient sample size for accurate estimation purposes. In MLM, apart from the general factors of sample size estimation, the test size, the effect size, SE (standard error) of the effect size and power of the test, additional factors like, magnitude of the ICC (Intra Class Correlation), total number of clusters, the number of parameters to be estimated, and the information whether the design is balance or unb...

Zahin Anjum1*, Farhat Shehzad1, Amina Rahat1, Hamid Ullah Shah2 and Saleem Khan3 

...not detected in all meat samples tested. It was concluded that factors like marination of meat and covered grilling significantly reduced the level of meat contaminants if, practiced with traditional grilling procedures. 

...

Abid Ali1*, Hidayat ur Rahman1, Farhatullah1 and Zahir Shah

...rch Institute (CCRI), Pirsabak, Nowshera-Pakistan. Parental inbred lines were crossed with each other to get six F1s during kharif 2015 which were selfed to develop F2s and backcrossed with their parents to obtain backcrosses BC11 and BC12 generations during spring 2016. The total experimental material in this study consisted of three parental inbred lines, six F1s, six F2s, six BC11, six BC12 and check cultivar P30K08 which were evaluated at two locations i.e...
Hayat Zada and Ahmad-ur-Rahman Saljoqi*
...sica campestris, Brassacicacae), Glycine max, leguminacae), Trifolium alexandrinum, Fabaceae) and Triticum aestivum, Poaceae) in the apple orchard were a substantial effect on the management of C. pomonella (L). Minimum mean fruit drop were recorded for the intercrop Apple + Trifolium (2.87) which were significantly lower than all other intercrops including control (Apple sole). Likewise, minimum percent infestation (57.19%) wer...
Iram Gull*, Muhammad Shahbaz Aslam, Imran Tipu, Roohi Mushtaq and Muhammad Amin Athar
...n PCR is used to make versatile chimeric gene products. However, the results of this technique are not always reliable due to inefficient overlapping of the sequences especially those derived from authenticated native sequences. Certain forbidden sequences do appear at junction of spliced genes whenever efficiency of gene fusion is improved by inserting GC rich sequences. In this study, reliable scar-less gene fusion with the insertion of sequence of choice at...
Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1
...i were isolated from 285 samples including 125 tonsil swabs (4-6 weeks old healthy pigs from 5 farms), 80 tissue samples from different slaughter houses (20 each intestine, liver, meat and kidney), and 80 samples from super- and wet-markets (each 20 meat and liver) collected both in summer and winter. Isolates were tested for 15 antibiotics (CRO, AMX, GEN, STR, TET, CHL, CLR, LVX, OFX, GAT...
Syeda Ain-ul-Batool1, Sadia1, Kathrin Blasius2,3,4, Angela Kaindl2,3,4 and Ghazanfar Ali1,*
...e BBS (BBS1-BBS21). A consanguineous family having clinical symptoms of BBS9 is described in current study. Mutation was detected in BBS9 on chromosome 7p14.3 using whole exome sequencing (WES). A splice acceptor site mutation (c.1131G>A) in exon 3 was revealed by Sanger sequencing.
...
Asma Yousafzai1,*, Muhammad Luqman2, Nisar Ahmed1, Muneeza Arbab1,Muhammad Murad1, Naheed Sajjad3, Sara Naudhani1, Shakeela Daud1, Abdul Hameed Baloch4, Khushnaseeb5, Sana Zahoor6, Zahid Rauf7, Muhammad Mohsin Javed6 and Jamil Ahmad1
...ts in Balochistan. Blood samples of 100 subjects including 50 breast cancer cases and 50 normal subjects were used to determine genetic variants in BRCA1 and BRCA2. Nine variants were identified in BRCA1 and four were identified in BRCA2. In case of BRCA1, six missense substitutions (p.Asp343Tyr, p.Gly393Asp, p.Ser561Phe, p.Ser616Phe, p.Pro871Leu and p.Ser1613Gly) two frameshift (p.Ser423fs and p.Gly1770fs) and nonsense mutat...
Shamsudin Bojang1, Idris Abd Ghani2, Jugah Kadir1, Adamu Saidu Paiko1, Yasir Iftikhar3 and Muhammad Kamran3,4,*
...al of 36 soils and roots sample were collected. Scanning electron microscopy (SEM) was used to identify the parasitic nematode. Both the field symptoms and SEM micrographs confirmed that the nematode isolate was M. graminis. Since this nematode has been known to damage the greens and other plants in other part of the world then the probability for the specie to adapt to other hosts other than the family of poaceae under Malaysian climate should not be d...
Sagir Hussain, Erum Iqbal* and Shahina Fayyaz
... About 280 root and soil samples were taken from 47 locations and 28 agricultural crops.Compilated data from the world has shown that Q. capitatus has the potential to survive and propagate proficiently at low temperature (-2 to -15ºC).
...
Abdur Rauf Nizami*
...al of 108 limestone rock samples were collected from 51 beds. The formation was investigated by a detailed petrographic analysis to ascertain its faunal assemblages in the Chichali Gorge Section. These investigations revealed that the limestones of this formation are a good repository of faunal fossils. The fossil contents found in the Samana Suk Formation display a wide range of faunal species. The documented species are comprised of: foraminifers, gastropods...
Pin Lyu1, Xiangxian Chen2* andQinlong Liu3*
...ontrol), exercise and massage, massage only and exercise only and received acute skeletal contusion to the right tibialis anterior, followed by corresponding rehabilitation methods. The injured muscular tissues and blood samples were collected pre-injury and post-injury on day 1, 2, 4, 6, 9, 12 and 15. The exercise and massage group showed the fastest re...
Lichao Feng1,2, Shaoqing Zhang4, Dianyuan Chen2, Sina Adl3 and Donghui Wu1,4*
...f insect in cropland landsacpe. The larva of click beetles (Agriotes fuscicollis Miwa) known as the wireworm is a serious underground pest in farmlands. The distribution of adults and larvae alternated between flower and corn growth stages in the field because food resources and foraging behaviors stimulated and restrained the acquisition of supplemental nutrients as a strategy for reproduction. To evaluate their food choices, wireworm were exposed to d...
Hong Zhang1, Shu-Fen Han2, Jing Wang1, Shao-Kang Wang1, Gui-Ju Sun1 and  Cheng-Kai Zhai1, *
...sugar levels, and at the same time improve obesity and fat accumulation in rats. CWD significantly augmented the relative level of peroxisome proliferators-activated receptor γ (PPARγ) and suppressed the sterol regulatory element-binding protein 1c (SREBP-1c) protein expression in rats’ tissues. Compound whole-grain ameliorates diet-induced obesity and hyperlipidemia by enhancing PPARγ and reducing SREBP-1c in rats....
Jun Yan Bai*, Shuai Yang, You Zhi Pang, Xiao Hong Wu and Guang Lu Li
...China yellow quail under same conditions was undertaken. Results demonstrated that egg weight, long end and egg shape index of Korean quail are significantly higher than those of Beijing white quail and China yellow quail (P<0.05). The short end, yolk height, yolk width and yolk weight of Korea quail and China white-feather quail are significantly higher than those of China yellow quail (P<0.05), the yolk index of China yellow quail is far higher than th...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
...lactating buffaloes were sampled. Data on several health, management and bio-security variables was collected in face to face interviews from farmers, managers and owners. Milk samples collected were subjected to California Mastitis Test.Data entry and validation was performed through Epi-Data. Data analysis was performed through SPSS. Chi-square and regression analysis were conducted. An overall prevalence of 67.3% was foun...
Riffat Sultana
...sh with black patches dorsally and brown ventrally.
...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*

Essossinam Ali* 

... allocation. Multi-stage sampling technique was used to collect data from 704 farm households in three regions (Central, Kara and Savannah) in subsistence agriculture in Northern Togo. Tobit and linear regression models were used to analyze the effect of farmers’ risk aversion on fertilizer and DTS uses, and labour allocation, respectively. The results indicate that on average 86.22% of farmers have risk aversion behaviors. Widening the land devoted to m...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
...ty vector vaccine. Blood samples were collected before primary immunization and at weeks 3, 5 and 7 after primary immunization and body weight of offspring were weighed at weeks 0, 2 and 4 after birth. Both SS-14 and SS-28 DNA vaccines induced humoral immune response, however, antibody response in T1 group were significantly stronger than that in T2 and C groups. Serum GH levels in T1 group was significantly higher than those in T2 and C groups, ...

Mehran Ahmad, Syed Attaullah Shah and Shahid Ali* 

...y data, collected from a sample of 105 tomato growers selected through multistage random sampling procedure. Data was collected through a well-designed interview schedule during January-July, 2018. Cobb-Douglas type production function was used to estimate effect of important determinants on tomato yield. Results showed that the estimated coefficient of net farming (dummy) is 0.25; implies that tomato yield with net farming ...
Muhammad Afzal1, Nighat Sultana1, Ali Hassan1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Syed Makhdoom Hussain4, Muhammad Bilal5 and Majid Hussain2
... meal (SBM) based diet.Basal SBM based diet was supplemented with two levels of CA (0 and 2%) and two levels of microbial PHY (0 and1000 FTUkg-1) in factorial arrangement resulting in the formulation of four experimental diets. Chromic oxide (1%) was added as an inert marker in the diets to evaluate the nutrient digestibility. Important water quality parameters such as temperature, pH and dissolved oxygen were monitored and maintained within accepta...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...injection (ICSI), as the savior of male infertility, has been applied to artificial assisted reproduction (IVF). The oocytes are inevitably ruptured by the microinjector in the process of microoperatin. To study the effects of mechanical damage from microopetations on embryo development, we analyzed 60 the spent culture media of porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The results showed that Group...

Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...g standard twig-dip bioassay method according to Completely Randomized Design. Results showed that both factors i.e. insecticidal treatments (F 9, 245 = 146.90, P < 0.001) and time (F 4, 245 = 445.75, P < 0.01) and their interaction (F 36, 245 = 9.20, P < 0.001) had a significant effect on the mortality of mealybug nymphs. The most effective novel chemistry insecticides against D. mangiferae mealybugs were sulfoxaflor, spirotetramat, thiamethoxam and ...

Imdad Ali Mahmood1, Muhammad Imran2, Muhammad Sarwar1, Matiullah Khan1, Muhammad Aqeel Sarwar2, Shoaib Ahmed1 and Shahid Riaz Malik

...eld were recorded. Plant samples were collected at maturity to determine Zn concentration in plant tissues and grains. A significant increase in pods per peduncle, seeds per pod, grain yield and total biomass of lentil was observed with Zn application even at lower rate (5 kg ha-1) under PGPR inoculation. Maximum 100-seed weight (2.56 g) was recorded with PGPR inoculation which was 21 % higher than that of without PGPR inoculation. Overall, a significant incre...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...eral compounds including salicylidene acylhydrazide, proteinase k, trypsin and chymotrypsin target bacterial virulence in Desulfovibrio spp. that causes bacteremia, periodontitis and abdominal infections. Ten soil samples were collected and screened by morphological and biochemical study. Only one strain DUV1 was confirmed by 16S rRNA gene sequencing as D. vulgaris (accession number: KY698020). It showed signif...

Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3* 

...he present study was envisaged to isolate the orthologs of SHPs and FUL from local canola “Pakola” and “Punjab Sarsoon 3”, and to study expression patterns of their transcripts. Morphological data revealed significant difference between pod wall thicknesses, seeds per pod and pod length between the two cultivars. PCR amplification and sequencing revealed that two products namely BnSHP1-like and BnSHP2-like could be identified. The seque...

Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassa

...23182 different examined samples of different species and sectors in Egypt, with a prevalence rate 4.4% by the year 2015/2016. However, the LPAI H9N2 showed a wide range distribution with high geo-prevalence rate in 2015/2016 (96.3%) as positive cases were recorded in 26 governorates., the positive samples were distributed in 783 farms with the highest prevalence rate (76.5%), Also, the most of positive cases were detected i...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...environment-friendly and safe alternate strategies to control D. mangiferae. To this end, the present study evaluated the methanolic extracts and essential oils of eight indigenous plant species for their insecticidal potential against 2nd instar D. mangiferae individuals. Standard twig-dip method was used for toxicity bioassays according to Completely Randomized Design. Mortality of mealybug individ...

Anam Sardar1, Iqbal Javed1, Abdur Rehaman2*, Mudassar Yasin3, Raheel Saqib4, Allah Bakhsh3, Haroon Javaid5 and Muhammad Luqman

Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3 

...tbreaks of Infectious bursal disease virus (IBDV) were reported in Egypt despite vaccination. Therefore, this study was conducted to characterize infectious bursal disease (IBD) viruses circulating in Egypt during the period of 2017 - 2018. Sixteen pooled bursal tissue samples were collected from IBD suspected (9 Egyptian balady and 7 broiler) chick...
Mohammed Anas Chowdhury1, Md. Abdul Karim1, Md. Tayfur Rahman1, Shoaibe Hossain Talukder Shefat1, Ashikur Rahman1, Mohammad Amzad Hossain2*

Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan  

...182 cloacal and tracheal samples were collected from suspected cases between 2015 and 2016 and submitted to the Reference laboratory of veterinary quality control on poultry production (RLQP) from different poultry sectors and bird species which are distributing all over the Egyptian governorates for examination of LPAI (H9N2) virus by real time RT-PCR. The results confirmed positive samples from 1026 for H9N2 with preval...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...L-1). After desalting specific activity of hepatic CAT remain lowest in exposed fish as 134.30±0.71 Unit mg-1in relation to control (148.02±0.41 Umg-1). The fold purification of hepatic CAT from control and exposed C. striata was noted as 2.35±0.01 and 2.22±0.01, respectively. The percentage recovery of hepatic CAT from control and exposed C. striata was calculated as 81.77±1.41a...
Huma Naz1*, Sajid Abdullah2, Khalid Abbas2, Muhammad Rizwan Tariq3, Laiba Shafique4, Ghazala Nazeer5
...lso evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla against END+CPF was computed as 1.35±0.01µgL-1(LC50) and 2.25±0.02µg...
Farah Bilal*, Abdulmohsen Alhejaily, Shahida Husnain
...g direct sequencing with sangers’ method. Only one sample detected p.E545K mutation at c.1633G>A. Another most common mutation was observed in PIK3CA exon 20 which leads to change A>G at codon 1047. This transition converts amino acid histidine to arginine. Our study concluded p.H1047R most frequent while p.E545K rarely found mutations in PIK3CA gene in NSCLC population. Present study can be proved ...
Mohsen M. El-Sherbiny1,2,*, Reny P. Devassy1, Erinn M. Muller3, Abdulmohsin A. Al-Sofyani1 and Ali M. Al-Aidaroos1
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
...accines of infectious bursal disease (IBD). Three experimental groups, each having 100 broiler birds were placed in the same environmentally controlled house. Samples of blood and bursa of Fabricius were collected from slaughtered birds after every 7 days till the end of experiment (35 days). The size of bursas and the bursa

Shumaila Sadiq1,2*, Abdul Saboor2, Fouzia Jamshaid3, Abdul Qayyum Mohsin2 and Azeem Khalid

Asad Ullah Khan1, Wiqar Ahmad1*, Amir Raza2, Farmanullah Khan3 and Muhammad Sharif3 

...ivum L.) genotypes under sand culture including Batoor-2007, Barsat (NRL 0320), Fakhr-e-Sharhad-99 (FS-99), Tatara-96 (WS 395), Paktunkhwa-15 (PR-103), NIFA Lalma (NRL 0517), Pirsabak 2013, NIFA Insaf (NRL 0707), Shahkar-2015 and Pirsabak 2015 (PR-105). Pots were arranged in completely randomized design in a glass-hous...

Muhammad Affan Khan* and Abdur Rab 

...bz Pari, Arka Anamika, Pusa Sawani, Punjab Selection and Green Star were grown at three plants spacing i.e. 20, 30 and 40 cm and were evaluated for growth and seed production. Variety Arka Anamika had the maximum plant height (136.8 cm), number of branches per plant (1.42), number of pods per plant (26.33), number of seeds per pod (60.67), seed weight per pod (3.87 g) and seed yield (4.54 t ha-1). The plant spacing of 20 cm resulted in the maximum plant height...
Xuefeng Cao1, Guangneng Peng1, Xiaobin Gu1, Changliang He1, Guizhou Yue2, Jun Shi3,* and Zhijun Zhong1,*
... CPV and CDV in clinical samples. We compared this new method against real-time PCR/(RT)-PCR and it showed no cross-reactivity with other pathogens. Sensitivity testing showed the minimum detection limits of real-time (RT)-PCR were 7.44×101 copies·μL-1 (CPV) and 4.20×101 copies·μL-1 (CDV). DARPM detection of CPV with DNA showed a minimum detectable of 1.53×101copies &m...

Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassa

... total of 8121 different samples (organs and swabs) that represented 588 NDV suspected flocks were collected from poultry farms showing respiratory manifestation and/or drop in egg production during 2015-2018. Out of 588 NDV outbreaks 95 flocks were confirmed positive NDV by rRT-PCR using genotyping primers, with a prevalence rate of 16.2%. NDV outbreaks were recorded in 16 governorates, from total of18 investigated governorates and the recorded geo-prevalence...
Proceedings of Pakistan Congress of Zoology
Proceedings of Pakistan Congress of Zoology
Jingchen Chen, Zhaochao Deng and Zhiqiang Han*
...he cost is basically the same. Based on this, we used the next-generation sequencing technology to measure the whole mitochondrial genome sequence of Ammodytes personatus, and compared with Sanger sequencing results. Three samples belonging to two lineages of A. personatus mitochondrial DNA (mtDNA) were used in the study. Based on the complete mtDNA sequence, the genetic distance between the two lineages is 0.0...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
...the availability of microsatellites especially from expressed sequences is currently very limited in this species and the genus Microhyla. A total of 17,339 potential microsatellites were identified in 15,385 unigenes which were generated by Illumina paired-end sequencing in M. fissipes. Within all microsatellites, AG/CT, AAG/CTT, and AAAG/CTTT are most prevalent motif in eac...

Muhammad Shafique, Nosheen Noor Elahi*, Muhammad Rashid, Amjad Farooq and Kausar Hussain Shah

...different NaCI levels in sand culture after 5,7 and 9 weeks of sowing. Both inoculated and uninoculated plants were grown on mineral medium that were N-free either without NaCI or with a range of NaCI (20, 50,100, 200 and 300mM). Dry weight of plants was increased at 0-50mM NaCl and decreased at 100-300mM NaCI concentration. Inoculation effectively increased the dry weights of plants at salinity levels of 0-50 mM NaCI as com...

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

...eria from water and soil samples collected from different glaciers of Karakorum Range of mountains, Pakistan. Serial dilution and plating approaches were exploited for bacterial isolation and the isolates were qualitatively screened for proteolytic and lipolytic activity with skim milk and Tributyrin agar (TBA plate) assay method. From the collected samples, a total of 20 bacterial isolate...

Usman Zubair1*, Anjum Shehzad2, Muhammad Ishaque Mastoi3 and Khalid Mahmood1 

...ic key by Mahmood and Hassan (2005) and pictorial key by Prabarker (2012). A total of 09 species of fruit fly of family Tephritidae from study sites were identified. Among these nine species, Bactrocera scutellaris, B. tau, B. nigrofemoralis, B. correcta, Dacus sphaerodalis are reported first time from Azad Jammu and Kashmir while D. longicornis is a new record from Pakistan and Azad Jammu and Kashmir. 

...

Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassa

...s study, a total of 7505 samples of tracheal swabs representing 1180 backyard poultry flocks were collected based on the surveillance conducted by the GOVS (General Organization for Veterinary Services), Egypt during the period from May 2017 to August 2018. 11 positive cases were confirmed in seven governorates with a prevalence rate of 0.93% and the positive samples were mainly located in Upper Egypt. The sequence of partia...

Munazza Yousra1*, Sair Sarwar1, Muhammad Mahmood Ul Hassan1, Muhammad Zameer khan1, Abdul khaliq2, Shahbaz Ahmad1 and Shamim Ul Sibtain Shah3 

...nSO4.7H2O and Zn-EDTA in sandy loam soil. Treatments included Zn @ 0, 2, 4, 6, 8 mg kg-1 as ZnSO4.7H2O and Zn-EDTA, arranged in completely randomized design with three replications. In Incubation study, the soil was incubated at 29ºC and field capacity was maintained to compare the release pattern of Zn from both sources. Zn release pattern from both Zn sources showed that Zn concentration was maximum on the first day in all treatments except control. Zin...
Gulzhan Аubakirova1,*, Zhanat Adilbekov2, Assylkhan Inirbayev2 and Tanatar Dzhamanbayev1
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...
Angelica polysaccharides (APs) has been used for many years in clinical practice and in blood invigoration. In this article, we want to elucidate the molecular mechanism underlying the effects of APs in the recovery of anemia. Red blood cell count and hemoglobin level in peripheral blood were measured using a fully automatic blood cell counter. Flow cytometry was performed to detect the changes in red blood cell counts and number of T cells in peri...
Wen-Feng Li1, Rong-Yue Zhang1, Chun-Hua Pu2, Jiong Yin1, Zhi-Ming Luo1, Xiao-Yan Wang1, Xiao-Yan Cang1, Hong-Li Shan1 and Ying-Kun Huang1,*
...eys on Saccharicoccus sacchari (Cocherell), Baliothrips serratus Kobusand Trochorhopalus humeralis Chevrolat. Protecting these natural enemies will encourage natural control of pest species while protecting the environment and maintaining ecological balance. Moreover, through enhanced comprehensive pest control, sustainable development of the sugar industry will be promoted.

 

...
Nazia Qamar* and Sher Khan Panhwar
...ianus (414), S. lysan (15), S. tala (8), S. tol (344) and Megalaspis cordyla (277) were collected from the commercial catches from July 2013 to March 2015. Fish length and otolith length for five species recorded as S. commersonnianus TLcm = 16.3−88.4, OLcm = 0.3−0.9; S. lysan TL=23.2−73, OL = 0.2−0.7; S. tala TL = 48.5−5...

Waqas Raza*, Muhammad Usman Ghazanfar and Muhammad Imran Hamid 

... of late blight infected samples. During 2016 and 2017, prevalence of disease in all the surveyed districts ranging between 34.6-24.4% and 39.3-28.6% respectively with district Okara recording the maximum (39.3%) and district Jhang the minimum incidence (24.4%) for both the years. Similarly, disease severity ranging from 19.3-16.1% and 21.1-17.4% during the year 2016 and 2017 was recorded with Okara recording the maximum (21.1%) and Sahiwal the lowest blight s...
Naheed Ikram and Nafisa Shoaib*
...cillium sp. and Fusarium sp. from 14 different genera of commercially important marine fishes Acanthopagrus sp. Parastromateus niger, Nemipterus sp., Pampus argenteusIlisha sp., Alepes djedabaEpinephelus sp., Teraponjarbua, Terapon puta, Scomberomorus koreanus, Epinephelus coioides, Lutjanus sp., Pomadasys sp. and Lutjanus johnii procuredfrom ...
Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2
...d PCR. In this study 720 samples were collected from different diagnostic centre’s/laboratories of Pakistan for ELISA and PCR tests. Out of 703 dengue positive samples, 93.5% were anti-dengue IgM positive, 71.5% were anti-dengue IgG positive and 81.3% were anti-dengue NS1 positive. Among these seropositive sample...

Ayub Khan* and Ghaffar Ali 

...elicit households WTP. A sample of 109 households was randomly selected from 14 randomly selected villages two from each tehsil. The study results revealed that most of the respondents perceived the problem of soil erosion and their willingness to pay for the suggested forestation based soil conservation practices were on average Rs.4666 per year. Regression analysis revealed that slope of the land, family size, income, land size, education, age and perception...
Yan-Bin Zhu1, Wang-Dui Basang1, Zhan-Dui Pingcuo1, Yang-Ji Cidan1, Sang Luo1, Dun-Zhu Luosang1, Yang-La Dawa1 and Guang-Xin E2,3,* 
...on using twenty-one microsatellites. The results revealed that the HO ranged from 0.4854±0.0194 in NNV to 0.6086±0.0267 in YRY, and the NA ranged from 3.86±1.98 in XMY to 6.05±3.37 in NNV. The least number of markers which deviated from Hardy-Weinberg equilibrium within the population was W, and largest number of deviations was in the NNV population. Consistent of phylogenetic relationships between the seven populations was identifi...
Shujjah Haider1,*, Ayesha Maqbool1, Tariq Pervez1, Saima Parveen1, Arfan Ahmad2, Zahid Iqbal3, Javed Iqbal3, Shahid Mehmood3, Amanullah Khan4 and Sajid Umar5
...an. A total of 358 blood samples were collected from different regions of district Chakwal and subjected to serological tests including enzyme linked immusorbant assay (ELISA) and rapid plate agglutination assay (RPA). The overall seroprevalence of MG detected through iELI...
Arif Jan*, Abdul Rab, Haroon, Rooh Ullah, Tauheed Ullah and Ikram Ullah
..., 0.8065 and 0.9120. The same value for association between body length and body weight was 0.9749. All the values are highly significant showing strong correlation. The GSI values ranged from 2 to 13.5. Highest value was observed in the month of March while lowest value is recorded in the month of April and October. Females to males ratio was found to be 1.04 (P>0.05). The study provides basic information about the reproductive potential and behavior of
Qudrat Ullah1,8,*, Huma Jamil1, Laeeq Akbar Lodhi1, Zafar Iqbal Qureshi1, Shakeeb Ullah1, Tariq Jamil2, Iahtasham Khan3, Shahbaz Bashir4, Qudratullah5, Inamullah Wazir6M. Abdus Sallam1 and Muhammad Zubair7
...on. A total of 384 serum samples were collected from cross bred dairy cattle at three different private farms located in Gujranwala, Pakistan. The samples were analysed by Rose Bengal Plate Test (RBPT) and by indirect-Enzyme Linked Immunosorbent Assay (iELISA). The overall seroprevalence was found to be 28.90 and 27.86...
Hui Wang1, Lin Wang1, Xuanlu Li1, Shanshan Li1, Yongqiang Zhao2, Shicheng Lv2, Xinrong Xu1, Guang Yang1 and Bingyao Chen1,*
.../sup>area is approaching saturation, and more and more birds have begun to utilize the buffer zone. We conducted a line transect survey in December 2010 to estimate bird density and evaluate the importance of the buffer zone. As a result, 34 protected species among the 4975 individuals were sighted, including 127 red-crowned cranes (Grus japonensis) and 868 common cranes (Grus grus). The overall density of protected species, winter migratory spec...
Zil-e-Huma1, 2, Abdul Malik Tareen3, Kaleem Ullah3, Tauseef M Asmat4,Abdul Samad4Asim Iqbal2, Mohammad Zahid Mustafa3*,Irshad Ahmad5 and Sadeeq ur Rahman6
...Pakistan. A total of 200 samples were collected from children suffering with diarrhea. E. coli was isolated using standard biological procedures and confirmed by colonial characteristics, biochemical profile and specie specific PCR. Multiplex PCR was applied to differentiate among the five main diarrheagenic E. coli pathotypes (enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), ...
 

Aftab Shaukat1,*, Khalid Medmood3, Irfan Shaukat2, Tauseef ur Rehman3, Muhammad Ahsan Naeem1,5, Ashar Mehfooz1, Muhammad Ijaz Saleem1, Zia-ud-Din Sindhu1, Shahid Ali Rajput4, Mubashar Hassan1,4, Saqib Umar1, Muhammad Ali Jamil1, Rao Zahid Abbas1 and Anas Sarwar Qureshi1

 

...attle of the district Faisalabad of Punjab province, Pakistan. Therapeutic efficiency of Oxytetracycline-Imidocarb dipropionate combination was compared with Enrofloxacin-Imidocarb dipropionate against Anaplasma marginale (A. marginale). For this study, the blood samples of three hundred and sixty nine cattle were collected. Giemsa stain of blood smears showed 10.84% infectio...
Salahud Din1,*, Saima Masood1, Hafsa Zaneb1, Habib Ur Rehman2, Saima Ashraf1, Imad Khan3, Muqader Shah4 and Syed Abdul Hadi1
...iographic analysis of tarsal bones in twenty specimens of male and female adult chinkara (Gazella bennettii). The tarsus of chinkara comprises of five bones both grossly and radiographically, settled in three transverse rows viz, tibial and fibular tarsal in the proximal, central and fourth fused tarsal in the middle row, while, the first, second and third fused tar

Attaullah Khan Pathan1, Imran Ali Rajput1*, Agha Mushtaque Ahmed2, Muhammad Waseen Kalroo1, Muhammad Munir Shahid4, Abdul Qadir Baloch3 and Faheem Ahmed Rajput2 

Masooma Munir1,2*, Abdul Ahad3, Asra Gull1, Aqsa Qayyum2, Nouman Rashid Siddique2, Amer Mumtaz2, Naeem Safdar2, Barkat Ali2, Muhammad Nadeem1 and Tahir Mahmood Qureshi

... fiber contents remained same during storage. The mineral and ash contents increased significantly (p < 0.05) among the treatments. Total sugars ranged from 64.77% to 67.29%. Reducing sugars were high than non-reducing sugars. It has been concluded that the addition of spinach has positive effect on acceptability and nutritional value of bars. 

...
Khalid P. Loneand Irfana Liaqat*
...h were kept in ponds and sampled every month for the histology of the testis. After taking data regarding other somatic traits, the testes were fixed in buffered formalin and processed for routine histology and light microscopy. The annual maturity stages can easily be divided into five gross stages namely, immature and developing, maturing and developed, ripe and running, post spawning and spent-regressed. The testis in Labeo rohita were unrestricted a...
Raja Imran Hussain* and Iqra Yousaf
...their uncontrolled dispersal threatening yields of various crops including wheat. The goal of this study was to evaluate the shifts of rodent abundances among five sets of three different habitat types (human dwellings, agricultural and non-agricultural land). It was also expected that trapping in non-agricultural land and human dwellings could reduce wheat damage. 30 trap stations were placed in each cropland, non-agricultural land and human dwellings for twe...

Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Gul Bahar Khaskheli2, Allah Jurio Khaskheli3, Arshad Ali Khaskheli4, Abdul Samad Magsi5, Ghulam Shabir Barham2, Arab Khan Lund5 and Maqbool Ahmed Jamali4 

Ghaleb Adwan*, Sami Bdir and Kamel Adwan
...div>Melanopsis praemorsasnails are considered one of the intermediate hosts for digenetic trematode cercariae. Digenean larvae were obtained from M. praemorsa snails that were collected from Palestine. Cercaria melanopsi palestinia I and III were identified as a xiphidiocercaria belonging to the microcotylae sub-group and as a microcercous cercaria, respectively. Phylogenetic analyses based on sequences ...
Riyadh S. Aljumaah1, Mutassim M. Abdelrahman1,*, Moez Ayadi1 and Abdullah H. Alyemni2
...ate were recorded. Blood samples were collected regularly during the different stages and analyzed for different important nutritional metabolites. In addition, colostrum, at parturition, and milk samples on day 30, 60 and 90 postpartum were collected for nutrient contents. The results showed an improvement in lambing rate of ewes in T1 and T2 (81 and 80%, respectively) compared to the control (78.8%). Moreover, ewes in T2 s...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...investigated using PCR assay for FAdVs. The FAdVs were detected in 8 samples collected from different flocks. Positive PCR amplicons were sequenced to genetically identify the FAdVs. Phylogenetic analysis revealed two distinct clusters among FAdVs. One cluster containing 3 strains belonged to the FAdV- C species and serotyped as FAdV-4 and showed close proximity at the nucleotide level. The other cluster containing three str...
Suhu Duan, Liuan Li*, Zaiqiang Li, Mengran Qin, Zhenzhen Fan, Keyan Zhang, Qian Wang, Zhongmou Zhang, Bailiang Yang
...lk, including 6 types of saturated fatty acids (SFA), 12 types of unsaturated fatty acids (UFA), 6 types of monounsaturated fatty acids (MUFA), and 6 types of polyunsaturated fatty acids (PUFA). There are no significant differences in content of total fatty acid (FA), SFA, MUFA, PUFA, ω-6 PUFA, and ω-3 PUFA among the three types of eggs (P...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...nzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It was found that more than ~85% mass of enzymes, migrated along with freezing front and concentrated in the centre in case of radial freezing. To examine the effect of unidirectional freezing over extracted enzymes, the working act...

Muhammad Usman Ghazanfar, Muhammad Imran Hamid, Mubashar Raza, Waqas Raza*, Misbah Iqbal Qamar 

...g dual culture and pot assays against Phytophthora infestans (late blight) and Fusarium oxysporum (wilt) of tomato on different compost including carbon rich compost, nitrogen rich compost and nutrient enriched compost. The species of Trichoderma includes T. harzianum and T. asperellum isolated from rhizosphere of tomato from different localities of district Sargodha, Punjab, Pakistan which were recognized morphologically. T...
Constance Obiageli Ejilibe1, Helen O. Nwamba2, Ifeanyi Chinedu Atama3,*, Chiamaka Lynda Ani2, Ifeanyi Oscar Aguzie3, Josephine C. Madu3 and Christopher Didigwu Nwani3
...ns in homogenized muscle samples was determined by standard procedures. ALT, AST and ALP increased significantly in response to concentrations of Butaforce® (7.00, 9.00 and 11.00 µgL-1) and Termex® (15.00, 20.00 and 25.00 µgL-1) used. The TP decreased in response to the same concentrations of pesticides. The response of these biochemical parameters were dependen...
Hafiz Suboor ul Hassan and Saira Malik*
...pulation. A total of 160 samples were collected and tetra primer ARMS-PCR was used for genotyping. 79.48% lactose intolerant individuals showed homozygous LCT-13910CC genotype and 16.66% showed heterozygous CT genotype. For LCT-22018G/A, 43.75% lactose intolerant individuals showed homozygous GG genotype, while 43.58% patients showed heterozygous GA genotype. Thus, different variants of genotype -13910T/C and -22018G/A of the individuals were found to be assoc...

Rashid Iqbal1,2, Mathias Neumann Andersen1*, Muhammad Aown Sammar Raza2, Muhammad Adil Rashid1 and Salman Ahmad2 

...m was irrigated with the same amount of water as applied to DI, and subsequently, irrigation was switched to the second half. PRD irrigation significantly improved WUE (both photosynthetic and intrinsic) and instantaneous carboxylation efficiency of Rubisco than FI and DI. Higher Abscisic acid (ABA) production under PRD reduced stomatal conductance, net photosynthesis and transpiration rate. Magnitude of osmotic adjustment (i.e. leaf water, osmotic and tugor p...

Zubaria Malik, Zahir Shah* and Muhammad Tariq 

...summer season (T4). Root samples of wheat and maize were collected at harvest stages and assessed for AMF colonization. Soil samples were also collected at the time of root sampling and analyzed for AMF spores. The results showed that neither root colonization of AMF in wheat and maize nor AMF spores density in soil were significantly (P<0.05) affected by cropping systems in any year. T...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5 
...airy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 42°C temperature. Gross and microscopic examination was carried out subsequent to the semen collection. Semen specimen with more than 70% motile characteristics were used for further processing. The semen was split into four aliquots in which tris-citric acid extender (TCA) was supplemented with various concentrations of L-cy...

Muhammad Noman, Yong Tong Mu*, Muhammad Mohsin, Aamir Mahmood Memon and Muhammad Talib Kalhoro

...n. Therefore, it is necessary to evaluate fisheries resources prior to harvest. In this study, two specialized fishery software’s viz. CEDA (catch and effort data analysis) and ASPIC (a stock production model incorporating covariates) are used to analyze the fishery output (FO) and fishery input (FI) data of Scomberomorus spp. to evaluate their fishery stock status in Pakistani marine waters from Balochistan coastline.Three surplus p...
Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*
...on across intestinal mucosa of hens using the Ussing chamber system. First, four Loman laying hens with similar weight (1.25±0.13 kg) were sacrificed to detect the lactate dehydrogenase (LDH) activity of isolated small intestine at time on 0 min, 30 min, 60 min, 90 min and 120 min, to provide a appropriate operation time for Ussing chamber test. Then, a total of 20 Loman laying hens with similar weight (1.25&plus...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
...istrated with a serial dosage of PFOS for 90 days for testicular observation and spermatogenesis evaluation. PFOS ≥ 11 mg/kg (accumulative dosage) resulted in visible histopathological changing in testis, including disorder and vacuolization of spermatogenic cells. Changed testicular organ coefficient (for dosage ≥ 55 mg/kg), decreased sperm concentration (for 110 mg/kg do
Abd El-Nasser Ahmed Mohammed1,2,*
...0 and 2.0% of Nigella sativa (N. sativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil)...
Kun Wang1,2, Yinglin Cui2, Xu Zhao2 and Changjiang Hu1*
... was measured by TUNEL assay. Using immunofluorescence, the expression of claudin-5, ZO-1 and VE-cadherin was detected. The expression of DNA methyltransferase 3b (DNMT3b) and Matrix metalloproteinase-9 (MMP-9) was evaluated by western blot. And microRNA-29b was identified using quantitative real-time PCR. 3). Compared with the Model group, the group treated with XN had a significantly reduced number of dead neurons in hippocampus and cortical regions of ICH r...
Muhammad Saeed1,2,*, Tariq Mukhtar1 and Malik Abdul Rehman3
... roots also followed the same pattern. The number of nematodes in the soil and females in the roots were the higher during the months of April to June and August-September showing two peaks throughout the year. The regression analysis between temperature and number of nematodes in the soil and females in the roots showed highly significant results at both the orchards. A direct relationship was observed between nematode population and temperature. Maximum nema...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *  
...process. Poultry litter, sawdust and rice husk were mixed in equal quantities by volume. One was treated with a single strain of Bacillus; another was treated with a consortium of bacteria comprising several species; and the third kept as the control without adding any bacteria. The results showed that decomposition started earlier in the treated batches than in the control group. During the fermentation period, there was no influence between the intern...

Muhammad Amin1,2*, Khalid Mahmood2 and Imran Bodlah3 

...istan. Astegopteryx bambusae (Buckton, 1893) is also represented as new record for the country. Data also have 3 second-finding, 6 third-finding, 11 new host plants and 71 new locality records in Pakistan. Aphis gossypii and Aphis craccivora were found infesting 8 tree species in 8 genera and 6 in 6 genera respectively. Rosaceae with 5 species in 4 genera carrying 9 aphid species in 5 genera was the most aphid-prone family. ...

Haidar Ali1, Malik Muhammad Shafi1*, Himayatullah Khan1 and Hamra Haidar

...ect observations. Random sampling technique was used for selecting a sample size of 195 small farmers. The present study is focused on two selected districts of Peshawar Valley namely Mardan and Peshawar. Analysis showed that there was a significant difference in the off-farm employment among various sizes of farms. Sampled farm households who were operating farm size up to 1 acre (74.88 hours/week) were performing more off-...
Hafiza Mehwish Iqbal1, Shahid Yousaf1, Salman Khurshid1*, Qurrat Ul Ain Akbar1, Saqib Arif1, Nasreen Fatima2 and Ali Murad Rahoo3
...thogenic diseases. Fruit samples (injured and healthy) were taken from the three different local markets of Karachi. Some of the fungal species viz. Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Rhizopus stolonifer and Alternaria alternata have been identified in citrus samples. However, major incidence was recorded for Aspergillus niger (41.6%) followed b...
Sikander Ali1, Muhammad Kamil Malik1, Muhammad Zubair1*, Muhammad Jawad Saleem2, Kanwal Hanif2 and Saira Azmat3
...hasis is being laid upon saving the crop from insect pests infestation. Scientists are continuously striving their best for improving the quality of the produce. Therefore, the present study was conducted during 2014-15 to evaluate the efficacy of different insecticides against the mustard aphid; responsible for huge yield losses in Brassica juncea. Maximum mortality of mustard aphid was caused by nitenpyram (96.24%) followed by carbosulfan (94.69%) and...
Jam Nazeer Ahmad1*, Taiba Sharif3, Samina J.N. Ahmad2, Sumaira Maqsood4 and Fariha Zafar3 
...ra zonata, Bactrocera dorsalis and Bactrocera cucurbitae was done in Punjab, Pakistan through PCR analysis using mitochondrial cytochrome oxidase I (COI) gene based primers. Phylogenetic analysis was also complemented to differentiate fruit flies identified from other countries of the world. The sequencing results and phylogenetic analysis of collected specimens indicated that sequences of B. dorsalis, B. cucur...
Nouf Alharbi*, Mai Elobeid and Promy Virk
...l orally received normal saline, Group II orally administrated cadmium chloride (5 mg/kg bw/d), Group III orally administrated cadmium chloride (5mg/kg bw/d) and treated with low dose of quercetin (50 mg/kg bw/d), Group IV orally administrated cadmium chloride (5mg/kg bw/d) and treated with high dose of quercetin (70 mg/kg bw/d). Experimental period was four weeks. The protective efficacy of quercetin was evaluated in terms of cadmium (Cd) accumulation in live...

Rizwan Ahmad and Muhammad Zulfiqar* 

...mbination of multi-stage sampling and simple random sampling used to select five Union Councils (UCs), one village council from each UC and one village from each village council. Out of 611 households (HHs), 180 head/elders selected through random sampling process were interviewed. The secondary data, spread over 30 years from 1984 to 2013 pertaining to temperature, precipitation, area und...

Sanaullah and Urooba Pervaiz* 

... data collected from 166 sample respondents who got training from Bajaur Area Development Program (BADP) using a well-structured interview schedule; through personal interview method. Result showed that 63.9% of the respondents were illiterate, 31.3% respondents were in age group between 25-35 years. Yield, income, plant spacing, seed rate, irrigation interval, and use of pesticide before and after training were computed with the help of paired

Zara Ahmad1*, Fazli Rabbi2, Muhammad Zamin3, Bushra Kiran2, Tariq Shah2 and Shahzad Kouser4 

...ata was collected from a sample of 110 farmers in the district of Faisal Abad, Punjab Pakistan. The data was analysed using Ordinary Least Square (OLS) technique. The results show that there is a significant difference between the on-season and off-season application of pesticides to bitter guard crop. The results clearly reveal that bitter guards’ yield is significantly higher in the off-season than on-season. It was ...

 Aftab Khan1, Shahid Ali1, Murtaza1, Sufyan Ullah Khan2 and Syed Attaullah Shah1

...loyed a multistage stage sampling technique for choosing the sample size. The data was collected from 200 respondents using a well–designed interview schedule. Cobb-Douglas type production function was modelled to estimated allocative efficiencies of individual inputs used in maize production. The estimated allocative efficiencies of Urea and DAP were 3.24 and 2.04, respectively. This implies that these inputs were und...

Naveed Afsar* and Muhammad Idrees 

...s of the two purposively sampled districts as these two were the highly vulnerable areas from climate change. The major source of climate change information of the majority of farmers was electronic media and the overall regression analysis of the climate change perceptions reveal that climate change is happening and long-term mean temperatures have been increased with a significant decrease in the long-term mean rainfall over the past decades. The study recom...
Tanzeela Riaz1, Farah Rauf Shakoori2,*, Hareem Mansoor1, Sana Khan1 and Mushtaq A. Saleem1
...ctin, abamectin and spinosad alone and in various conbinations such as abamectin:emamectin (1:1, 2:1, 3:1, 1:2 and 1:3) and abamectin:spinosad (1:1, 2:1, 3:1, 1:2 and 1:3) against two larval instars (4th and 6th) of MBDIN and Lahore populations of T. granarium. The LC50 of abamectin, emamectin and spinosad for 4th larval instars of Lahore ...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...e Linked Immunosorbant Assay (ELISA) using HCV antihuman conjugated protein. It was confirmed that the HCV3a E2 purified protein bound with the human serum almost three times more efficiently than the positive control. For raising antibodies against the antigenic protein HCV3a E2, the purified protein E2 was injected subcutaneously into the rabbit (Oryctolagus cuniculus). The...
Chen-Yu Sun, Wen-Chao Liu*, Mei Xiao, Zhi-Hui Zhao and Li-Long An*
...(linear, P<0.05), serosal layer thickness, muscular thickness, and wall thickness of duodenum (quadratic, P<0.05), and the serosal layer thickness and wall thickness of jejunum were also improved by inclusion of different level of betaine (cubic, P<0.05). Moreover, dietary supplementation of betaine increased the length of ileum (quadratic, P<0.05). In conclusion, the betaine could be used as growth promoter for ...

Syed Muhammad Hassan Raza1*, Syed Amer Mahmood1, Syeeda Areeba Gillani1, Syed Shehzad Hassan1, Muneeb Aamir1, Muhammad Saifullah1, Mubashar Basheer1, Atif Ahmad1, Saif-ul-Rehman2 and Tariq Ali1 

...t along with open source satellite datasets. We downloaded three images of each year (2008-2018) from Geological Survey of United States and applied geometric corrections. All the spectral wavelengths were transformed to Top of Atmosphere reflectance and processed the bands in infrared and red wavelengths to generate Ratio Vegetation Index (RVI) and Normalized Difference Vegetation Index (NDVI) datasets. The annual NDVI and RVI based rice yields were compared ...
Muhammad Saleem Hanif1, Khalid Javed Iqbal1,*, Arshad Javid3, Noor Khan2, Irfan4, Hamid Majeed5, Muhammad Altaf3
...n South Punjab. Head Taunsa and Punjnad were selected as study sites to collect information from fishermen through well prepared questionnaire. It was observed fishermen in both study sites were of 20 -55 year of age having maximum family size of 7-10 individuals. Illiterate fishermen were recorded to be 50 and 40 % at head Punjnad and Taunsa, respectively. Fishermen maximum yearly income at both study sites were in the rang...

Sajjad Ali1, Atta-ur-Rahman1 and Sher Ali2* 

...paring 1985 and 2015 Landsat images. Secondly, four sample union councils were targeted for medium level analysis. Thirdly, seven sample villages are targeted for micro level. The Landsat images of 1985 and 2015 are obtained from the open source Global Land Cover Facility (GLCF). The analysis revealed that the cultivated area was 146 square kilometer in ...

Masood Ahmad* and Abdur Rab 

...wever application of the same concentration of calcium at 7th leaf stage resulted in maximum size and weight of daughter corm. It is concluded that foliar application of 200 mM calcium at 7th leaf stage increased the size and weight of daughter corm and hence could be recommended for better corm production in Peshawar conditions. 

...

Muhammad Sharif1*, Sadullah1, Ghulam Rasool2, Muhammad Nasir Khan1, Zubair Rehman1, Ahmed Khan2, Ijaz Ahmad3 and Khalid Hussain4 

...ifferent treatments were same. 

...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
...iceatannol in human osteosarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that pi...
Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*
...were isolated from urine samples (55%) followed by pus (28%), blood (7%), sputum (6%), Broncho-alveolar lavage (2%), fluid (1%) and from tips (1%). The isolated organisms were Escherichia coli (n=60), Klebsiella pneumoniae (n=20), Enterobacter cloaca (n=09), Enterobacter aerogenes (n=02), Proteus mirabilis (n=05), Proteus vulgaris (n=02) and Citrobacter freundii (n=02). Extended spectru...
Fiza Asif1,*, Muhammad Siddique Awan1, Nasra Ashraf1, Nuzhat Shafi1, Abdul Rauf1, Khizra Bano1, Muhammad Razzaq2 and Naeem Iftikhar Dar2
...l technique. Sixty fecal samples were collected from the study area during summer 2015 and winter 2015-16, i.e. thirty samples during each season. These samples were analyzed for the determination of food composition using microhistological technique. A total of 23 plant species were observed during summer and 15 plant species during winter season. During both the seasons Indian or ...

Muhammad Zeeshan Manzoor1*, Ghulam Sarwar1, Mukkram Ali Tahir1, Noor-Us-Sabah1, Ayesha Zafar1 and Sher Muhammad2 

...plants to soil and water salinity depends upon climate, soil characteristics, topography and management strategies. Water, even may be saline, is becoming more precious natural resource. Hence, there should be more crops and jobs per drop while conserving the quality of present terrestrial and groundwater. Saline water has been used for production of fodder and forages in many countries. Managing soil and water

Muhammad Ali1*, Norsida Man2 and Farrah Melissa Muharam

...subjective norms on ICT usage among farmers for the management of agricultural risk in Malaysia. Therefore, the present study was designed to meet the objective. The research data were collected through a field survey in which 360 farmers were interviewed through the multistage cluster sampling technique. The subjective norms were measured through 5 points Likert scale (ranging from 1 as strongly dis...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...in structure of clinical sample, while in subclinical samples, showing the same variation in overall 3D protein structure analysis of TNF-α gene. The association between polymorphism identified within the TNF-α gene with mastitis reported in this study revealed that SNPs has potential to serve as a molecular marker for screening of mastitis resistant and susceptib...
Yacheng Hu1,2, Xueqing Liu1,2, Jing Yang1,2, Kan Xiao1,2, Binzhong Wang1,2 and Hejun Du1,2,*
...novel cross-species microsatellite markers for Dabry’s sturgeon via next-generation sequencing from Chinese sturgeon (Acipenser sinensis). The 17 microsatellites were polymorphic with 3 to 6 alleles per locus and the total number of alleles is 80. The mean expected heterozygosity (HE, 0.506 to 0.781), observed heterozygosity (HO, 0.542 to 1), Hardy-Weinberg departure value (
Laiba Shafique1,*, Muhammad Afzal2, Syed Zakir Hussain Shah3, Mahroze Fatima4, Huma Naz5, Saif ur Rehman1, Youchuan Wei1 and Qingyou Liu1,*
... dissected to obtain the samples of muscles for further analysis. Two-way analysis of variance under RCBD were applied on obtained data. Results demonstrated that growth performance (weight gain, specific growth rate and absolute weight gain) of C. idella fingerlings showed significant improvement by feeding formic acid and vitamin D3. Result showed that C. idella fed with vitamin D3 increased the crude protein, ash and decr...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
... Enterobacter aeruginosa, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus intermedius, Xylose staphylococcus all showed different degree of inhibitory effect. The inhibitory effect of the protein monomer on gram-positive bacteria was stronger than that on gram-negative bacteria, but not on candida albicans. This novel and monomeric APP-1 has antibacterial ...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...is, in primordial granulosa cells, primary, secondary, and antral follicles of ovary, and none in theca cells. The more conspicuous reaction for BMP15 was observed in germ cells than cumulus cells and granulosa cells, particularly in primordial germ cells of genital ridge or in foetus ovary of buffalo. The expression pattern of BMP15 suggested that it may play a key role in the formation of primordial follicles...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...ant candidate genes. DNA samples of Marecha camel were collected from the Camel Breeding and Research Station at Rakhmani Bhakar, Pakistan. Two significant polymorphic sites were identified in GH gene and three in GHR gene. Out of these, T1720A polymorphism in GH and A211927G polymorphism in GHR genes changed the amino acid from leucine to histidine and methionine to valine, respectively. These polymorphic results suggest that Marecha camel, a major breed in P...
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...fragments and three microsatellite markers. When using mtDNA loci, 242 individuals (44.24%) were excluded for no shared haplotype with broodstock. Further 81 (14.81%) crabs were identified as hatchery-reared individuals based on microsatellite loci from the remaining 305 crabs. Our results also showed high genetic diversity and a certain degree of heterozygote deficiency of natural swimming crab populations. The advancing te...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
...oluble Ash method. Feces samples were taken and analyzed in the same day for Salmonella spp. and E. coli and blood samples for analysis were taken from the subcutaneous abdominal vein. The experimental rations were almost identical in chemical composition. Results of nutrient digestibility and nutritive value, as total digestible nutrients (TDN) and digestible crude prot...
Abdulkareem Mohamed Matar1, Moez Ayadi1,2, Hassen Mohamed Sbihi3*, Imeddine Arbi Nehdi3, Mutassim Mohammad Abdelrahman1 and Riyadh Saleh Aljumaah1
... (C12:0 + C14:0 + C16:0) saturated fatty acids were significantly lower in milk fat from ewes fed TF (45.67%) and CF1 (42.13%) compared to CF3 (53.65%) and CF2 (49.67%). Linoleic acid (C18:2∆9c,12c; n-6) was significantly higher in milk fat from ewes fed CF1 (4.17%). While, no significant difference was detected for α-linolenic acid (C18:3∆9c,12c,15c; n-3). The percentage of trans-vaccenic acid (C18:1∆11t) was signi...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Naimat Ullah Khan3, Muhammad Asfandyar Khan2, Kashif Prince1,*, Mahboob Ali1, Ghazunfar Rashid1 and Azmat Ulah Khan2
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...e located in district Faisalabad, Pakistan. A total of 455 blood samples were collected from slaughtered cattle and buffaloes at random and then tested by a commercially available ELISA kit for the detection of antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Data were analyzed using chi square for ELI
Mohammed Al Bratty1,*, Hassan A. Alhazmi1,2, Desam Nagarjuna Reddy3, Abdul Jabbar Al-Rajab3, Sadique A. Javed1 and Zia ur Rehman1,4
... present study, propolis sample was collected from adjacent to Jazan city, Saudi Arabia and the chemical constituents of the ethanolic extract was evaluated by GC-MS. The ethanolic extract was tested against selected strains of microorganisms for its antibacterial and antifungal activities. The major classes of compounds indentified in the extract were fatty acid esters and aliphatic hydrocarbons. Methyl-(7Z)-7-hexadecenoate, methyl-(9E)-octadecenoate and meth...
Abdul Hanan1, Talha Nazir1, Abdul Basit1, Shahbaz Ahmad2 and Dewen Qiu1,*
...V-5) with different bioassay materials (conidia, filtrate and the binary combination of conidia + filtrate) were evaluated against green peach aphid (Myzus persicae) under control condition. Conidial bioassay of fungal isolates were evaluated with three different concentrations (1×106, 1×107 and 1×108 conidia/ml), filtrate bioassay ...
Muhammad Zameer Khan1, Sair Sarwar1*, Ahmad Khan1, Razaullah Khan1, Munazza Yousra1 and Muhammad Ilyas2
...TPA soil test method can safely be used for evaluation of K status of soils derived from diverse parent material.
...

 Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*

...n as measured by CCK-8 assay, Sircol assay, qPCR and western blot. MSCCM also reduced the expression of the pro-fibrotic TGF-β1 and increased the expression of anti-fibrotic IFN-γ at both mRNA and protein levels. Furthermore, we found that MSCCM inhibited the activation of NF-κB signaling pathway induced by irradiation as well. Our data suggest that MSCCM could reduce irradiation-induced TGF-β1 producti...
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...in a static renewal bioassay for 48 hours and 96 hours (96 hours L.C-50 0.22 mg/L). The study was conducted on 40 fish that weredivided into 4 groups A, B, C and D. Group A was considered as control group.Group B, C and D were exposed to sublethal concentrations of methylmercury 0.044 mg/L, 0.055 mg/ L and 0.073 mg/L in tap water respectively. The fish exhibited tail and fin disruption, bulging of eyes, scales erosion, mucous secretion, hemorrhage, color chang...
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
...from eye infections. The samples were taken from corneal eye infections. The collection of samples was done from “Layton Rehmat Eye Hospital, Township Lahore”. Isolated bacterial pathogens were identified on the basis of morphological and various biochemical tests. Morphologcal tests included different staining procedures includng Gram’s staining and Endospore staining. Identification  of pathogenic ba...
Khalid Khan1*, Hazrat Yousaf1, Noor Mohammad1, Munir Khan1 and Do Thi Thao2
...food security, real disposable income of households’and food production. The results of the study also revealed that the implication of monetary policy will be a significant instrument to influence poverty, food security, and consumption patterns in China. 
...
Manzoor Hussain*, Marie Maňasová, Miloslav Zouhar and Pavel Ryšánek
...ledge, repetition of the same crop in field, and non-awareness of pesticides applications. Ten isolates from seven different fungi, Arthrobotrys oligospora, Dactylella oviparasitica, Clonostachys rosea, Stropharia rugosoannulata, Lecanicillium muscarium,Trichoderma harzianum and Pleurotus ostreatus,along with two chemicals,Vydate and Basa<...
Ambreen Leghari1,2, Asghar Ali Kamboh2,*, Shakeel Ahmed Lakho1, Faiz Muhammad Khand1, Kanwar Kumar Malhi2,3, Iqra Bano Chandio4, Seema Baloch5 and Jan Mohammad Shah4
...nism from the milk and nasal secretions of cattle using the Lowenstein-Jensen media. An overall prevalence of 34.38% was recorded by rapid test that was higher (P< 0.001) than SITT (3.13%) and culture test (2.50%). A somewhat higher prevalence was recorded in Hyderabad district (SITT 3.75%, Rapid 36%, culture 3.75%) as compared to Tando Allahyar district (SITT 2.5%, Rapid 32%, culture 1.25%). In Hyderabad district, rapid test showed a significantly higher (...
Muhammad Usman Akhtar1,2, Abdul Qayum3, Anshan Shan1,*, Shuli Chou1, Hyeonsoo Jo4, Syed Waqas Ali Shah4 and Ishfaq Muhammad5
...ere determined and blood samples were taken at 3, 6, 9 and 12 h post feeding, and examined for BUN. In addition, milk produced by each goat was recorded. The results showed that increasing RDP level in the diet has a linear effect (dose-dependently) on nutrient digestibilites, nutrient intake and milk yield. Dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) intake were higher (P < 0.05) in goats fed 70RDP diet...
Qudsia Yousafi1*, Muhammad Aslam2 andShahzad Saleem1
...auty, Hybrid Shilpa, Bemisal and Nirala were used in the experiment. The number of aphids and ladybeetles were recorded at weekly intervals from October 15 to March 1, both years. Aphid numbers were recorded on three leaves per plant and four plants per plot and averaged per leaf. Numbers of ladybeetles were recorded from the whole plant by sampling three plants per plot. Seasonal mean number of aphids per leaf was at the ma...
Ahmad-Ur-Rahman Saljoqi1, Imtiaz Khan1*, Bashir Ahmad1, Fazal Maula2 and Sardar Hussain1
... by increase with the passage of time and reached to peak till week 4th (5th May). Then, the population of aphids started declining and lowest population was observed in week 8th (2nd June). Data regarding the natural enemies revealed that, the population of natural enemies was low at the beginning, increased till week 4th (5th May) where highest population was recorded. Afterwards declined tren...
Muhammad Mansoor1, Khalid Usman2*, Shahid Hameed Khan Khalil3, Abdul Mateen Khattak4, Muhammad Ehsan Elahi1, Muhammad Waqas Imam Malik2 and Amir Hamza2
..., Pakistan for quick and safe ripening of date fruits under controlled conditions. For ripening of date fruits, 35 ± 5°C temperature and 80 ± 5% relative humidity; and for drying, 45 ± 50C temperature and 30 ± 5% relative humidity levels were kept constant. The moisture contents were reduced to recommended moisture of 23-25% for shelf life stability. Data for fresh date production ranged from 39-59% of the initial val...
Muhammad Ramzan1, Unsar Naeem-Ullah1*, Muhammad Ishtiaq1, Ghulam Murtaza2, Mirza Abdul Qayyum1 and Faisal Manzoor1
...eek of September while disappeared in the last week of October. The study revealed that the natural enemies were observed rarely in month of May, early stage of crop. The study resulted that weather parameters like temperature, humidity and rainfall play significant role in fluctuation of natural enemies.
...

Faleye Temitope Oluwasegun Cephas1,2*, Adewumi Moses Olubusuyi1, Olayinka Olaitan Titilola3 and Adeniji Johnson Adekunle1,3 

... could be EVs lurking in samples with NR-CPE. Fifty-nine cell culture supernatants (CCS) (collected between 2016 and 2017) from RD and L20b cell-culture-tubes with NR-CPE, were analyzed. The tubes had been previously inoculated with stool suspension from children (<15 years) in Nigeria with acute flaccid paralysis (AFP). All CCS were screened for Enteric Adenoviruses (EAVs) and group A Rotavirus (GARV) using a rapid immunochromatographic test kit. Subsequen...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
... Western blot, cell TG Assay and RT2 Profiler PCR array. The results showed that ABCA1 knockdown reduced the TG content in BMECs. On the other hand, the RT2 Profiler PCR array demonstrated that the expression levels of 12 genes, including fatty acid binding protein 4 (FABP4), acyl-CoA dehydrogenase short/branched chain (ACADSB), ELOVL fatty acid elongase 3 (ELOVL3) and solute carrier family 27 member 1 (SLC27A1), were altered upon ABCA1...

Adewumi Moses Olubusuyi1, Ogunsakin Temitope Rachael1, Ogunrombi Sefiu Babatunde1, Ojeamiren Iduda1, Olawole Alaba Samuel1, Faleye Temitope Oluwasegun Cephas1,2* and Adeniji Johnson Adekunle1,3 

... 30 stool suspensions (2 samples per case) were analyzed as 60 pools (pooled per case by cell line). Pools were subjected to RNA extraction, RT-snPCR, amplicon sequencing and phylogenetic analysis. Eleven and one of the 30 pools of RD and L20B cell culture supernatants, respectively, were positive for the RT-snPCR. Nine of the 11 amplicons from RD and the only from L20B were sequenced and identified as seven EV types; Coxsac...
Himanshu Mishra, Vikas Kumar and Ashish Kumar*
...ry birds at Bakhira bird sanctuary, district Sant Kabir Nagar, Uttar Pradesh, India from October 2017 to March 2018. Thirty two species of birds were observed during the field investigation. The line transect method was employed for population estimates. During the field survey, we recorded a significantly higher number of migratory birds at the end of early winter (December) and at the commencement of middle winter (January). Red crested pochard (Netta ruf...
Shad Mahfuz1,2, Shuyuan Wang1, Mo Chen1, Fei Zao1, Dong Zhen1
Zhongjun Liu4 and Hui Song1,3*
... treatments included a basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW for 8 wk. Data were subjected to one-way analysis of variance using SPSS software followed by Duncan’s test probability of p<0.05. During the experimental period no significant differences were observed in egg production, egg mass, egg weight, feed intake and feed conversion ratio (FCR) among groups. Among the egg quality parameters haugh uni...
Muhammad Khaled Siddiq1, Fatima Yousuf Dar1,M. Akbar Khan1, Muhammad Adeeb Babar1,*, Sayyed Ghyour Abbas1, Asra Ghaus1, Musdassir Iqbal1 and Muhammad Akhtar2
Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Umar Mumtaz1, Muhammad Zafarullah Khan3 and Abdul Naveed4
...re identified from which sample of 120 respondents was randomly selected. The data were collected by using interview schedule. Descriptive as well as inferential statistics were employed for interpretation of the desired results. Significant association was observed between age groups of the senior farmers and capacity building approaches or styles used by the senior farmers to train and educate young farmers.
...
Faheem Aslam1*, Aneel Salman1 and Inayatullah Jan2
...ant challenges in out of sample predictability tests due to model uncertainty and parameter instability. Recent studies introduce several machine learning algorithms to improve time series prediction accuracy. The purpose of this study is to develop a precise wheat production model using artificial neural networks (ANN). A total of 71 years’ wheat production data from 1948 to 2018 is divided into training data and test data. The model is trained by ...

Muhammad Abu Bakar1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Rashid Mahmood2, Ziyad Abdul Qadir2, Muhammad Arshad1 and Mubasshir Sohail3 

Tasleem Akhtar1,2,Ghazanfar Ali1,*, Nuzhat Shafi2 and Abdul Rauf2
...length of 16S rRNA in 45 samples ranged from 1527 to 1552 bps. Five haplotypes (h) were observed, with haplotype diversity (Hd) 0.5323±0.080. Among 5 haplotypes, 2, 2 and 1 haplotype were detected in S. plagiostomus, S. niger and S. esocinus, respectively. Haplotype diversity was lowest and same in S. esocinus, S. niger and S. progastus (0.0001) and highest in S. plagiostomus (0.5436...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...ing three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the lesions of all examined tissues. Strong positive staining was primarily fou...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

... lactate dehydrogenase assay kit; superoxide dismutase (SOD) activity and malonaldehyde (MDA) concentration were detected by a total superoxide dismutase test kit and a malondialdehyde test kit. Genomic DNA and RNA were extracted from cells; Methylation was inhibited by treating HL7702 cells with 5-aza-2’-deoxycytidine (AZA), and the level was analyzed via the methylation-specific PCR (MSP) methods. The increases in the methylation rate of CpG islands in...
Taimoor Khalid*, Asad Ullah and Mussawar Shah
...Pakhtunkhwa, Pakistan. A sample size of 357 respondents was proportionally allocated to the mentioned three union councils of the study area and selected by simple random sampling technique. Perception of the respondents regarding illegality in forest management (independent variable) and forest governance (dependent variable) were measured on three level likert scale. Chi-square test was applied to test the association betw...
Muhammad Zeeshan Majeed1*, Imtiaz Sarwar1, Muhammad Afzal1, Muhammad Rehan Khalid1, Muhammad Yahya1 and Khurram Shehzad2
...S) index. Extensive soil sampling was done in different randomly selected land-use types for four consecutive seasons from spring 2017 to Spring 2018. Results showed that the collection season, land-use categories and land-use types had a significant and differential impact on the diversity and population abundance of major soil arthropod groups. Spring and summer seasons exhibited the maximum diversity and abundance of soil arthropods, while minimum populatio...
Shagufta Naz1, Ayesha Javed1, Ayesha Saleem1, Khadija Murtaza1, Rukhama Haq1, Akbar Hayat2 and Neelma Munir1,*
...v>...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
...V from a Liver Center Faisalabad, Pakistan. A total of 3200 clinical samples were screened for the presence of anti-HCV antibodies by ICT method. Among positive samples, confirmation was carried out by RT-PCR and clustered into four groups (acute, chronic, cirrhotic and hepatocellular carcinoma) on the basis of clinical reports and the patient’s history. HCV genotypes were determined...

Muhammad Zubair1, Sagheer Ahmad2*, Awais Rasool1, Muhammad Asad Farooq1 and Ibni Amin Khalil3
 

Po Bunnika, Khalid Khan and Guo Xiangyu*
...eir profits by upsurging sales performance instead of improving technical performance. These results also confirm that cooperatives managers should give greater importance to the effective use of capital and labor by increasing agricultural output.
...
Abdul Majeed1*, Muhammad Anwar-ul-Haq2, Abid Niaz3, Abid Mahmood4, Naeem Ahmad1 and Hafiz Muhammad Walyat Ali Khan1
... was performed to screen salt tolerant wheat varieties grown in hydroponic and soil medium on the basis of plant photosynthetic rate, potassium (K+) and sodium (Na+) contents. Twenty wheat genotypes/varieties were grown in hydroponic culture, having different residual sodium carbonate (RSC), electrical conductivity (EC), and sodium adsorption ratio (SAR). A substantia...
Riaz Shah1* and Margaret Appleby2
...fore spray (contact bioassay) or after the spray (residual bioassay). In contact bioassay, all of the tested pesticides killed more than 95% of the TSSM after 72 hours except diatomaceous earth (DE). Diatomaceous earth killed only 24.6+3.2% TSSM that was significantly lower than all the other pesticides used. When mites were released onto treated leaf discs (spray residues), only garlic/ch...
Sadreddin Tusun*
... larvae of Cueta lineosa (Rambur 1842) is described and figured for the first time by scanning electron microscopy (SEM). The females were collected from Batman, Diyarbakır and Mardin Provinces in South Eastern Anatolian Region of Turkey and maintained in the laboratory. The different parts of the third instar larvae such as antennae, head, mandibles, abdomen and types of sensory receptors are described and illustrated.
...
Hande Kucukonder1, Pinar Celebi Demirarslan1*, Sezai Alkan2 and Özgür Baris Bırgul3
...f female and male growth samples, determining the best fitted growth model and designating the biological meaningful parameters (inflection point age, weight and growth rate) of growth functions. Analyses showed that the growth profiles of female and male chicks found not to be parallel using profile analysis, and the male chicks had a higher body weight than the females (P < 0.01) starting from 14-21st days until the end of experiment. For this ...
Zelle Huma1*, Ahmad-Ur-Rahman Saljoqi1 and Farman Ali2
...e laboratory. When  samples soil were collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post application. Dead larvae were then put on the white trap and EPNs has then collected afterward. It is concluded on the basis of results that EPNs is positively recorded in grassy land, tomato field, f...
Muhammad Nisar* and Muhammad Idress
... gardening study is necessary not only due to its social, food security and economic contribution, but its consideration’s negligence in development agendas. Home gardening data provide complete agricultural input in a nation’s domestic treasure. Therefore, the current study was carried out with the objective to assess small farmers’ involvement in home gardening at district Tank, Khyber Pakhtunkhwa- Pakistan. Three hundred and twenty (320) s...
Wiqar Ahmad1*, Farmanullah Khan2, Muhammad Sharif2 and Muhammad Jamal Khan2
...igher porosity (ƒ), saturation (ω) and available water (θ), respectively over the fertilizer control, whilst amongst cropping patterns, lentil after maize effect was significantly (p<0.01) superior over the wheat after maize and wheat+lentil intercrop after maize for improving soil properties. Significant correlation existed between bulk density and yield (r2=0.96 and 0.91) and soil OM and yield (r2=0.84 and 0.75) for ...
Muhammad Qasim1*, Said Qasim2, Muhammad Naeem1, Amir Nawaz Khan3 and Shahid Iqbal4
...nd 2016, in district Charsadda, Khyber Pakhtunkhwa, Pakistan. Three types of fishers were selected for the study i.e. farming fishers, fishing farmers and occasional fishers, through two stage cluster sampling. Livelihoods asset pentagons were used for the analysis. Considerable decline in physical and economic assets were noted among all the three types of fishers, whereas the total value of livelihood assets for farming fi...
Tahsin Razzaq*, Muhammad Fareed Khan and Shahid Iqbal Awan
...s Soan-3, Ghuari-122, Kissan-60 were evaluated as highly susceptible (S) within germplasm and had highest values for pathological traits with severity rating of 90S, 90S and 80S respectively. In second field trail (June 2017) crosses were made between selected parental genotypes in order to get F1 progeny and evaluation of F1 progenies and their parents was done in June 2018 for morpho-pathological traits to assess the impact of NCLB on y...
Rakhshanda Kousar1, Tahira Sadaf1*, Muhammad Sohail Amjad Makhdum2, Muhammad Amjed Iqbal1 and Raza Ullah1
... is having competitive disadvantage of Pakistan in case of production of mango, citrus and dates throughout the study period. In a similar token results of log of Relative Export Advantage Index in all years under consideration, reveals competitive disadvantage of Pakistan as value of this index remained negative in case of all the three fruits. If value of Revealed Comparative Advantage is compared in case of these three fr...
QihangLiu1,2, YueliJiang2, Tong Li2, Jin Miao2, Zhongjun Gong2, Yun Duan2, and YuqingWu2*
...t intensity but with the same light wavelength exceeded the visual tolerance range of the insects and strengthened the visual stimulation effect. Our results also indicated that the length of exposure time was important in determining the visual response and thoracic temperature change, suggesting that these features are key to improving the phototactic impact of light treatment. Thus, our results could be useful in developing light-based treatments to help co...
Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7
... purpose, a total of 100 samples of cloacal and 100 oropharyngeal swabs of ducks (Anas Platyrhynchos domesticus) were collected from different villages of four tahsils of Sheikhupura during period (May to July 2017). NDV was isolated from field samples by inoculating the suspected samples into embryonated eggs of 9-11 days. Isolated virus was confirmed through haemagglutin...
NaseemZahra1,2,*, Nadia Jamil1, Sajid Rashid Ahmad1, Imran Kalim2, MuhammadKhalid Saeed2, Ijaz Ahmed2, Qurat-ul-ain Syed2 and Soniya Munir1
...sent study a total of 80 samples were analyzed for Aflatoxin M1 using competitive ELISA kit method. Branded samples (n=40) were collected from different shops in Lahore city of Punjab Province while non-branded samples (n=40) collected from four selected towns of Lahore. Overall 58% and 100% of aflatoxin contamination ...
Jiaxin Guo1, Xiumei Zhang1,2, Xiaoyan Wang2, Liqin Liu2 and Tianxiang Gao1,*
...or potential useful microsatellite markers obtaining of S. japonica. A total of 120 microsatellite markers were designed and tested, 25 primer pairs showed polymorphism among S. japonica individuals. Number of alleles, observed and expected heterozygosity per locus ranged from 2 to 8, 0.083 to 0.922 and 0.042 to 0.866, respectively. The PIC ranged from 0.04 to 0.829. These markers will be useful in evaluation g...

Asma Hassan*, Zuhair Hasnain, Shahzada Sohail Ijaz, Muhmmad Ansar, Muhmmad Rasheed, Ajaz Ahmad, Lubna Ayub Durani and Muhmmad Bilal Ali 

...ient nutrient supply and satisfactory soil and climatic conditions. Moldboard plough (MP) (control), tine Cultivator (TC) and minimum tillage (MT) as main treatment, Crops sequence as subplot included were fallow–wheat (Triticum aestivum), (FW, control), mungbean (Vigna radiata)–wheat (MW), sorghum (Sorghum bicolor)–wheat (SW), green manure–wheat (GW) and mungbean-chickpea (MC) (Cicer arietinum). In summer in 2010-11 the highest nitroge...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...ium longiflorum) and chrysanthemum (Glebionis segetum) against the worker individuals of subterranean termite Odontotermes obesus (Isoptera: Termitidae). Bioassays were conducted using filter paper disc method according to completely randomized design with four replications for each treatment. Termite mortality data was recorded at 6, 12, 24 and 48 h post-treatment. Median lethal concentration (LC50) and median lethal time (...

Matiullah Khan1*, Motsim Billah2, Shoaib Ahmad1, Raza Ullah Khan1 and Muhammad Sarwar1 

...ed consecutively, in the same lay out of randomized complete block design with three repeats. Various doses of PEC (6, 4, 2 Mg ha-1) were compared with simple poultry-litter compost (PLC) as 8 Mg ha-1, single super phosphate (SSP) fertilizer as 100 kg P2O5 ha-1 and control. The results showed that PEC at 6 Mg ha-1gave 14.7% and 7.7% increased paddy yield and 14.7% and 11.9%-increased total dry matter yield over PLC at 8 Mg ha-1 and SSP, respectively. The highe...
Ahmad Ali1,2,* and Aleem Ahmed Khan1
... buzzard (Butastur teesa) as bait technique were recorded. Out of these eight techniques two i.e. sock-ball and laggar falcon (F. jugger) /white eyed buzzard (B. teesa) as bait technique were documented for the first time. The size of sock ball is about 7-8cm in diameter having noose traps of 7-8cm in size used to install on the surface.Laggar falcon (F. jugger) and white eyed buzzard ...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1 
...before in camel. The DNA samples of Marecha camel were collected from the Camel Breeding and Research Station at Rakhmani Bhakkar, Pakistan. Four polymorphic sites were detected in the IGF-1 gene. A significant finding was the occurrence of a T→C polymorphism in exon 5 that causes a substitution of an amino acid from Cysteine to Arginine.That Cys/Arg polymorphism may serve as authoritative genetic marker for selective improvement of growth traits i...
Mo Chen1, Shad Mahfuz1, 2, Yan Cui1, Liying Jia1, Zhongjun Liu3 and Hui Song1, 4*
...ry treatments included basal diet as a control group; control diet including antibiotic (0.05% flavomycin) as an antibiotic group; 2% FVS fed group; 4% FVS fed group and 6%FVS fed group. The experimental duration was total 63 days, from 30 wk to 39 wk. Serum total antioxidant (T-AOC) was significantly higher (P<0.05) in 6%FVS than control; glutathione peroxidase (GSH-Px) was significantly higher (P<0.05) in FVS fed groups and control groups than in the a...
Memis Ozdemir
...ny studies about Prl/RsaI polymorphism found in the exon 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic s...

Nosheen1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...ed for 4-day. After each sampling (24-hr interval) fish were dissected and required organs were obtained. Results showed that the POD activity was augmented in all selected organs of Pb+2 exposed fish in relation to control. The POD activity in organs of fish followed the order: brain>liver>gills>kidney>heart>muscle. Regression analysis showed a positive relationship between POD activity and duration of exposure. It was also concluded that antio...

Aamir Saleem1, Arshad Mahmood Malik2*, Najam Ul Hassan1 and Imtiaz A. Qamar

...t of Common Vetch (Vicia sativa L.) legume on growth and yield of Rye grass (Lolium multiflorium L). Data regarding the physical-chemical analysis of the soil (ph, electrical conductivity, total nitrogen, organic matter and available phosphorus), crop parameter (Number of tillers, number of leaves, plant height, fresh land dry weight and crude protein) and meteorological data regarding rain fall, temperature, pan evaporation, sunshine and wind speed for interp...

Muneer Abbas1*, Muhammad Ramzan1, Niaz Hussain1, Abdul Ghaffar1, Khalid Hussain1, Sohail Abbas2 and Ali Raza3 

... important role in field sampling, monitoring, capturing, killing and biodiversity studies of nocturnal insect population. Funnel shaped light traps were used in mungbean and gram crops throughout the year. Effects of light traps were assessed by daily night collections in relation with abiotic factors based on marginal cost benefit ratio. Results indicated that total 32415 insect’s captures were made with >26 insect species including 4 species of bio...

Muhammad Abdul Rahman1*, Abdul Saboor1, Gulnaz Hameed1, Ghulam Bilal2 and Farooq Tanwir3 

...mistic policy options to safeguard Pakistan’s dairy production system from environmental factor under climate change. 

...

Wajid Shah Yousafzai1, Irfan Ullah1*, Amjad Ali1, Mahmood Iqbal2 and Salman Khan1 

...t swat. Using multistage sampling technique cross sectional data was collected from 105 sample respondents in three villages (Aboha, Manyar and Parrai) of Tehsil Barikot, district Swat. Simple Linear Regression Model was employed to individual input contribution in total output. The concept of Marginal Value Products and Marginal Factor Cost was used to calculate Allocative Efficiency. Ordinary Least Square technique was use...

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

Muhammad Naeem Khan* and Asad Jan 

...ds, phenols, flavonoids, saponins, tannins and sterols, however in n-hexane fraction (HxF) only flavonoids and saponins were detected. In quantitative analysis, among all the solvents, ethyl acetate fraction (EAF) had highest amount of phenol (27.4 mg/g), flavonoids (15.5 mg/g) and alkaloids (3.14 mg/g), while MCE displayed maximum amount of saponins (34.3 mg/g). In the proximate analysis,...

Jahangir Khan*, Syed Attaullah Shah, Khurram Nawaz Saddozai, Muhammad Fayaz, Shahid Ali, Abbas Ullah Jan and Ghaffar Ali 

...of Khyber Pakhtunkhwa. A sample 150 apple consumers were interviewed at different fruits retail shops to collect data on apple varieties, prices and quality attributes, such as color, size, shape, freshness and external defect. The data were analyzed using hedonic pricing model. Results show that Consumers have strong preferences for apple freshness, size, color and juiciness and their willingness to pay for these attributes are significantly greater than zero...

Jahangir Khan1*, Abbasullah Jan1, Kar Ho Lim2, Syed Attaullah Shah1*, Aditya R Khanal2 and Ghaffar Ali1 

...view a randomly selected sample of 600 households. Survey data revealed that households were willing to pay on average rupees 26 more per kilogram of pesticides free fruits. Results from regression analysis showed that age of the household head and his awareness of pesticides use over fruits, household size and their total monthly income were significant determinants of their WTP. In addition, health concern and environment concern of pesticides use also influ...

Tanveer Ahmad1*, Muhammad Mujtaba Rafiq1, Waqas Ahmed Dogar2, Abid Mahmood Alvi3, Qumer Iqbal4, Muhammad Azam5 and Arshad Ali Khan6 

... growth and yield of Phalsa (Grewia asiatica L.). Healthy phalsa plants were fertilized with P2O5 @ 50g, 100g and 150g plant-1. Data were recorded on number of fruit bush-1, yield bush-1 (kg), single fruit weight (g), fruit diameter (cm), number of fruiting nodes, length of new shoot (cm), number of sprouted shoots cane-1 and leaf area (cm2). Treatments were applied under Randomized Complete Block Design (RCBD) in triplicate...

Muhammad Abdul Qayyum1*, Farhat Bashir2, Muhammad Mudassar Maqbool3, Anser Ali3, Saqib Bashir1 and Qaiser Abbas4 

...concentration of soluble salts. This study aims to examine the effect of NaCl salt at 100 mM and 200 mM concentrations on linseed (Linum usitatissimum L.) germination, plant growth, nutrients and soluble salts uptake. The results revealed that tested four linseed genotypes showed an overall germination rate of 86-94% at 100 mM NaCl (T2) and 78-84% at 200 mM NaCl (T3) and seedling survival ...

Raheel Saqib1*, Muhammad Luqman2, Iqbal Javed3, Abdur Rehman4, Muhammad Yaseen2, Saleem Ashraf5 and Muhammad Zeeshan Majeed2 

... the respondents was not satisfactory. Majority of the households had limited human, financial, physical, social and natural capitals. In the scenario of climate change, households adopted diverse nature of farm and non-farm related strategies to secure their livelihoods. Findings of chi-square statistics showed highly significant association (P<0.05) between agricultural diversification strategies being adopted by households in the situation of changing cl...

Sibgha Noreen*, Sumrina Faiz, Muhammad Salim Akhter, Kausar Hussain Shah 

...fferent osmoprotectants (salicylic acid, ascorbic acid, proline and their admixture) @ 200 mgL-1 on sunflower (Helianthus annuus L.) grown under saline environment (150 mM NaCl). The results showed that salinity stress (150 mM) resulted into significant decrease in biomass accumulation of sunflower up to 50%, while it was improved by 80% in control by the foliar application of osmoprotecta...

Fazle Akbar and Sahib Alam* 

...me on total fungal and Fusarium counts and mycotoxins zearalenone (ZON), trichothecenes type-A (i.e. T-2 and HT-2) and trichothecenes type-B (i.e. DON, 3-ac DON, 15-ac DON) production in maize grains. Water activity of the grains was adjusted to 0.85, 0.90 and 0.95 aw and the samples were stored at 25°C for 45 days. The control samples were kept without any aw modification. At 15 days ...

Shahid Ali1*, Farhan1, Murtaza1, Neelum Andaleeb2 and Amjad Ali1 

...tan. A four stage random sampling technique was used to collect data from 160 respondents. Stochastic frontier production (SFP) function was used to analyze the technical efficiency of wheat growers. The Maximum Likelihood estimates of the model-1 showed that seed quantity, urea, chemicals (Pesticides and weedicides), labor and tractor hours were statistically significant with the coefficients of -0.027, 0.090, 0.060, 0.223 and 0.056, respectively. The estimat...
Hanif Ullah1, Muhammad Inayat Ullah Khan2, Suleman3, Sawar Khan1,Salma Javed4, Abdul Qadeer1, Mohsin Nawaz1, Sardar Azhar Mehmood4
...n. A total of 1750 blood samples were collected from seven tehsils of District Lower Dir (January to December 2013). The data were analysed tehsils wise, month wise, gender wise, age wise and species wise. Thick and thin smear were prepared and examined under microscope. The data was statistically analysed in Microsoft excel. Out of 1750, 214 (12.2%) blood samples were found positive. The higher rate of infection was found u...

Olufisayo Bamidele* and Safiriyu Idowu Ola 

...eg;, respectively. Blood samples were collected pre injection and at 60 and 120 minutes post insemination. Data recorded were Plasma LH levels, kindling rate, total kit born, total born alive, still born, weight of kits at birth and gestation period (days) and were analyzed using Analysis of variance of the General Linear Model procedure and t-test where appropriate. The plasma LH for the AI groups peaked at 60mins while it peaked at 120mins for the control. K...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
...e in aspartate amino transaminase (246-308 U/L), alanine amino transferase (31-58 U/L) and blood urea nitrogen (11.21-22.58 mg/dl). 
...
Shaukat Ali1,*, Sadia Batool2, Fatima Javed Butt2, Sundas Nasreen1, Hafiz Muhammad Tahir1
...nd radiological drawback sand provides an appropriate cancer treatment strategy. Anticipating various challenges, we expect that probiotics may become a potential cancer bio-theranostic in the field of medical sciences shortly. Appropriate monitoring and treatment are strongly recommended to prevent hepatocellular carcinoma complications. 
...

Muhammad Israr* and Noor Khan 

... province of Pakistan. A sample of 336 wheat growers, consisted of equal number of FSC registered and non-registered farmers, was selected from Northern, Central and Southern climatic zones of Khyber Pakhtunkhwa. The selected wheat growers were interviewed for data collection. Binary Logistic regression analysis was conducted over the collected data-set to estimate a grower’s probability of adopting improved seed technology and quantify the role of FSC i...

Shwe Yee Win1, Lat Lat Htun1, Myint Myint Hmoon1, Hla Myet Chel1, Yu Nandi Thaw1, Nyein Chan Soe1, Htet Lin Oo3 and Saw Bawm2* 

...9. A total of 200 faecal samples were randomly collected from village chickens. Centrifugal sugar floatation and sedimentation methods were carried out to detect the gastrointestinal parasites. The overall occurrence of gastrointestinal parasites in village chickens was 60.5% (121/200). Six species of gastrointestinal parasites were observed as Capillaria spp. (30.0%, 60/200), Ascaridia galli (24%, 48/200), Eimeria spp. (20.5%, 41/200), Raillietina spp. (17%, ...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...evels pain, tiredness, disability, reduced distinction life. Precise pathogenesis job rheumatoid factor not known, through formation immune complexes engross establishment harmonize. To determine the correlation between Anti-Oxidants and Biochemical status in Rheumatoid Arthritis patients. 60 clinically diagnosed Rheumatoid Arthritis (RA) patients and 50 healthy persons were included in this study. 5 ml Blood was drawn and serum was separated. Anti-Oxidant Bio...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...Aflatrem, Monacolin K, Fusarubin and Tautomycetin. Different fermentation parameters were optimized to produce antimicrobial agents using Response Surface Methodology (RSM) and best results were obtained at 45°C temperature, 5.5 pH, 5 mL inoculum concentration, 1.5 mL banana peel as substrate and 20 days incubation time. The antimicrobial activity of secondary metabolites was tested against Streptococcus aglactaie, Streptococcus pyogene, ...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
...m fruits, soil and water samples obtained from different localities of Lahore city, Pakistan. Locally isolated microorganisms were screened for their mannanase producing characteristics on mineral salt agar medium pH 7. Six isolates were found to be mannanase producers by qualitative analysis. Three isolates (MBL-SP02, MBL-CW01 and MBL-GN02) produced maximum zone of hydrolysis with maximum activity ratio. These isolates (MBL...
Nadia Saeed1, Mian Sayed Khan2, Habib Ahmad3, Muzafar Shah4*
...sion, KP, Pakistan. Soil samples collected from walnut trees showed that Abbottabad and Mansehra had both parasitic and saprophytic nematodes whereas Kohistan had only saprophytic nematodes. Helicotylenchus sp. was found in abundance from Abbottabad and Mansehra alongwith other parasitic nematodes including Aphelenchus avenae, Tylenchus species, Pratylenchus species, <...
Ali Mujtaba Shah1,2,3, Ali Raza Shah3, Muhammad Farooque Hassan2, Muhammad Yousif2, Zhisheng Wang1*
...oglobulin, lactose, oligosaccharide, vitamins, minerals, growth factors and nucleotides and its benefits to health of animals.
...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...r a 10 days period. Milk samples were tested for aflatoxin M1 (AFM1) through high performance liquid chromatography (HPLC), somatic cell count (SCC) and total viable count (TVC). Blood samples were analyzed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels. Aflatoxin M1 (AFM1) was detected in all milk samples of the Group B, C, and D...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
...erial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the most effective compound. These results demonstrate the potential antimicrobial properties of mono an...
Shahid Sherzada1,2,4,*, Muhammad Naeem Khan4, Masroor Ellahi Babar2, Muhammad Idrees3, Abdul Wajid2, Muhammad Nauman Sharif3, Muhammad Shahzad Iqbal3, Iram Amin3 and Muhammad Shahid3
...f Punjab province. Blood samples of sixty (60) fishes were taken carefully from caudal veins of each captured fish species. Genomic DNA was extracted from blood and short segment COI gene (680bp) was amplified by PCR. The sequences were evaluated by using bioinformatics tools. The average K2P conspecific, congenric and confamilial genetic distances were 0.22%, 6.71% and 12.33%, respectively. Findings of the present study revealed that DNA barcoding usin...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...sis to intervene in necessary strategies to avoid future outbreaks in commercial poultry.
...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and viscera were dissected out for further analysis. Levofloxacin induced reduction in body weight while caused a noticeable increase in liver, kidney and testis weights. Significant increase in levels of total alkaline aminotransferase (ALT), aspartate transa...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...tic level of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and a significant increase in bilirubin levels in BP group. Significant raised patterns were recorded for urea and creatinine in BP intoxicated mice against control. Histological defects observed were necrosis, pyknosis, increased sinosuidal spaces, portal hypertension, glomerulosclerosis, abnormal vascular pole in liver and kid...

Mahnoor Pervez and Farkhanda Manzoor* 

...ues in pollen and nectar samples collected from various agricultural areas of Pakistan through high performance liquid chromatography analysis. Pollen and nectar were collected from 2016 to 2017 from different agricultural areas of Pakistan during a field survey. Pollen was collected by installing traps and nectar was collected through capillary method allied with centrifugation. The HPLC technique was used for fast and simple detection of pesticide residues o...

Turab Ali Kaurajo1*, Huma Rizwana1, Gulbahar Khaskheli2, Muhammad Haroon Baloch1, Muhammad Naeem Rajput1, Asad Ali Khaskheli3 and Mohsin Solangi

Imran1*, Amanullah1, Muhammad Arif1, Zahir Shah2 and Abdul Bari3 

... rates were remained the same for weeds biomass. When compared with the economic analysis and profitability of soybean the highest net returns (NR) in Pakistani Rupees (PKRs) (PKR 62.082 ha-1) were noted with the biochar amendment followed by compost (PKR 60,168 ha-1), whereas least net return NR (PKR 41,548 ha-1) was recorded with peach residues incorporation. The value cost ratio (VCR) was highest with compost application (5.48) among the organic sources fol...

Manzoor1*, Ahmad Khan1, Amir Sohail2, Shahzad Ali1, Fawad Ali Shah3, Junaid Iqbal3, Muhammad Owais Khan4 and Sultan Nawaz4 

... harvest (61316.60), thousand grain weight (205.90 g), leaf area (422.92 cm2), grain yield (2948.35 kg), biological yield (10562.30 kg). While broadcast planting produced high Shelling percentage (46.90). The interaction of planting methods and deficit irrigations, maize grow on ridge method and give full irrigations produced higher plant height (192 cm), ridge and one irrigation missing at six leaves stages had more Harvest index (35.06%)) and high Shelling p...
Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1
...her capra species. Blood samples were collected from Kirthar National Park, Sindh, Pakistan. PCR product was sequenced bi-directionally using dideoxy chain termination method after mitochondrial ATPase8/6 genes amplification. Polymorphism, Genetic diversity and Phylogenetic analysis were carried out by the MUSCLE, DnaSP and MEGA6 tools. Total of 20 variations at different positions were found in aligned sequence results. Sequence conservation was observ...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
...d Pseudomonas aeruginosa. Seven different types of antibiotic discs were used to check the intrinsic resistance of all the isolates to various antibiotics. Interestingly, most of the isolates were found resistant while only a few were susceptible, however, the isolates Lysinibacillus spp. N40 and Brevundomonas spp. P20 showed no activity at all. Increased levels of resistance to heavy metals such as; iron, arsenic, cadmium, chromium, and n...
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslamand  Samina Jam Nazeer Ahmad1, 2
...t P450 gene. Insect bioassay indicated highly significant level of LC50 values among laboratory reared and field collected population against Cypermethrin. The laboratory reared resistant population, Sindh and South Punjab collected population showed high LC50 values as compared to susceptible, Baluchistan and KPK population. The expression of P450 gene through PCR showed enhanced level of P450 gene in different Instars as well as collected population. The dsR...

Arjumand Nizami1, Jawad Ali2 and Muhammad Zulfiqar

...re crucial in terms of disasters with likelihood of spring / monsoon floods and winter drought. 

...

Syeda Naila, Muhammad Ibrar, Fazal Hadi* and Muhammad Nauman Khan 

...features showed that palisade ratio was (7.35), vein islets number (9), and vein termination number (9.1), stomata number (47) and stomatal index were (42.72). Transverse section of the root showed outer epidermal cells, cortex and pericycle, xylem and phloem cells. Powder drug microscopy, ash analysis, extractive values and fluorescence analysis were also studied. Preliminary phytochemical screenings of different extracts of Oxalis pes-caprae L. showed the pr...
Fariha Latif1*, Muhammad Javed2, Hammad Ahmad Khan2 and Khalil-Ur-Rehman3
...ach fortnight, fish were sampled from each treatment and different organs such as liver, kidney, gills muscles and brain were taken out for determination of bioaccumulation and oxidative stress in the fish. The results showed that water-borne Pb exposure caused significant accumulation in the fish tissues that increased significantly with increasing exposure of Pb. Pb accumulated variably in the fish tissues as liver > gills > brain > kidney > musc...

Sanaullah1, Fazli Rabbi2*, Shahid Ali2, Zeeshan Khan3 and Muhammad Zamin4 

...l that 98 percent of the sampled tourists were willing to pay for the development and conservation in the area. Moreover, 37 percent of the sampled tourists were willing to pay Rs.100 (0.94 US$) per visit as an entry fee. The analysis further showed that an estimated amount of 421 million Rupees (3.97 million US$) could be generated as consumer surplus. The poisson regression estimates show that visitors’ frequency to ...
Zübeyde Kumbıçak1,* and Hatice Poyraz2
...i> using a classical Giemsa staining method. We collected specimens from different populations in Nevşehir and used totally fourteen male spiders. The two Drassodes species had karyotypes comprising 10 pairs of autosomes plus sex chromosomes which were X1X20 (♂) type. All chromosomes including sex chromosomes were telocentric and relative lengths of autosomal pairs were decreased gradually in size. All male meiosis was chiasmate...
Sumaira Abbas1, Muhammad Sultan Haider2,*, Fatima Kafayet1, Sana Ashraf2, Atifa Masood3 and Moazma Batool4
...tography (TLC) and found satisfactory. By mixing fish meal, sunflower meal and rice polish four treatment diets, T1, T2, T3, were prepared and citrus peel powder was added @ 200g, 400g and 600g/ 1000g, respectively. While fourth one, control diet (To) was without citrus peels. These is nitrogenous diets (30% protein) were offered to Carassius auratus juvenile having body weight 20±7.54g in powder form f...
Tanweer Kumar1, Sahib Zada2,3,Muhammad Irfan4,*, Huma Batool5 and Wasim Sajjad2,6
.... A total of 4,758 blood samples from the mixed population were collected including 2382 male 2376 females. All the samples were subjected to hepatitis B surface antigen Immuno-chromatographic test (ICT), HBsAg enzyme-linked immunosorbent assay and by Reverse transcription polymerase chain reaction for the existence of antibodies against HBV. It was observed that 307 (12.8%) out of the 238...
Saman Muhsin Abdulkareem* and Nadir Mustafa Nanakali
... the study, animals were sacrificed and their liver were removed for biochemical analysis including the level of oxidative stress biomarkers, liver enzymes, and the expression of CYP1A1 gene. The results of this study indicated that exposure to TCDD (10µg/kg/day) could significantly increase liver oxidative stress biomarkers and serum liver enzymes (p<0.05). While, pre and post treatment of QCT (20 mg/kg/day) had protective effects on hepatotox...
Zekeriya Demir1 and Hatice Kaya2*
... control diet (Pol-0), basal diet plus 0.5% (Pol-1), 1.0% (Pol-2) and 1.5% (Pol-3) bee pollen. The present study lasted for twelve weeks. During the experimental period, the hens were fed ad-libitum and water was available all the times. The hen house was lit for 17 h. The layers fed with diets including BP showed linear increases in final body weight and body weight changes calculated from initial and final body weights at the end of the trial. The sup...

Hafiz Abdullah Shakir1*, Javed Iqbal Qazi2, Abdul Shakoor Chaudhry2, Muhammad Irfan3, Muhammad Khan1, Shaukat Ali4 and Saima Shahzad Mirza5 

...two flow seasons at four sampling locations including upstream Lahore Siphon = A, Shahdera = B, Sunder=C and downstream Balloki headworks = D. All the metal contents in fish scales were highly significantly different (P<0.001) among sampling locations and flow seasons. Location-wise metal accumulation pattern was C > D > B > A. The highest concentrations (µg/g dry weight) of Cd (0.29), Cr (4.64), Cu (8.85),...

Urooba Pervaiz1*, Madeeha Iqbal1 and Dawood Jan2 

...d to select the required sample, 80 farmers were randomly selected from five villages and data were collected through interview schedule. It was found that majority farmers were literate up to middle; young farmers showed more positive attitude towards extension recommendations, 93% farmers knew extension workers both by name and face and also knew extension recommendations, but they faced problems like small land holding, high prices of inputs, poor financial...

Rahat Naz*, Mussawar Shah, Asad Ullah, Intikhab Alam and Younas Khan 

...universe of the study. A sample size of 370, was randomly selected from the total population of 10142 through proportional allocation method. The conceptual framework comprised of two study variables i.e “agriculture production” and “climate change effects on human life”. To established association between the study variables the perception of climate change effect on human life was indexed and cross tabulated with local response to agr...

Hassnain1, Abdul Basit1*, Mehboob Alam1, Imran Ahmad1, Izhar Ullah1, Noor Alam2, Inayat Ullah3, Muhammad Areeb Khalid1, Muhammad Shair4 and Noor ul Ain1 

...eld. Application of chitosan helps to induce drought resistance and improved water use efficiency. Therefore, a research study was planned to investigate the effect of chitosan on growth and yield of tomato (Cv. Rio Grande) plant under water stress condition at Ornamental Nursery, Department of Horticulture, The University of Agriculture Peshawar, Pakistan, during 2018, using Completely Randomize Design (CRD) with two factor...

Roshan Ali1, Afsar Ali2, Shamsher Ali2, Muhammad Arshad Khan3*, Haroon Shahzad3, Noman Latif3, Muhammad Waheed4, Ashraf Khan5 and Murad Ali6 

Awais Bin Shahid1, Moolchand Malhi1,*, Saeed Ahmed Soomro1, Muhammad Giasuddin Shah2, Nazeer Hussain Kalhoro3, Asmatullah Kaka4, Rajoo Mal1, Muhammad Awais Soomro1Saba Parveen Samo1 and Muhammad Nawaz Sanjrani5
...oncentrate (2 % BW) as basal diet without (C) or with selenium yeast (SY, @ 0.3 mg.Se.kg-1.diet) supplementation. Hay and water was provided ad libitum. The results revealed that the molar concentrations of propionate and the total short chain fatty acid (SCFA) increased (P < 0. 005) and the pH decreased (P < 0.05) in the ruminal fluid of SY goats compared to C. The mor...
Khursheed Ahmed, Shoaib Freed*, Rana Fartab Shoukat and Kanwar Waqas Ahmad
...m anisopliae and Isaria fumosorosea and six synthetic insecticides i.e., triazophos, imidacloprid, bifenthrin, nitenpyram, pyreproxifin and lambda cyhalothrin against O. hyalinipennis. The efficacy of these microbial agents could be enhanced by applying in combination with insecticides. Five concentrations i.e., 1×106, 1×107, 1×108, 2×108 and 3×108
Nazir Ahmad Khan1,*, Mudassir Alam1, Rafiullah khan1, Kamran Khan2 and Sadeeq ur Rahman3
...vivo digestibility assay showed that QPM300 had higher (P < 0.05; 81.7%) dry matter digestibility, followed by diets containing QPM200 (78.3%) and QPM-mix (78.2%), while lowest (P < 0.05) digestibility was recorded for the control diet (74.1%). The three QPM based diets did not differ in N retention; however, they retained higher (P < 0.05) percentages of nitrogen (51.4-53.9%) than the control diet (43.9%), suggesting higher amino acids availabili...
Asima Rani1,*, Syed Kashif Nawaz2 and Muhammad Arshad3
...ivax exposure. Blood samples of 228 malaria patients and 226 healthy controls were selected from the local population. Malarial samples were divided in to complicated malaria (N=89) and mild malaria (N=139) groups according to WHO criteria. Malarial groups were further divided into P. vivax and P. falciparum groups based on the Plasmodium species responsible for the infection. Allele specific PCR was...
Hazrat Ali1,2,*, Ezzat Khan1,* and Muhammad Jamal Nasir3
... different environmental samples were collected from the river, downstream the city of Peshawar, and analyzed for the selected heavy metals by atomic absorption spectrophotometry. Heavy metal accumulation was studied in muscles of six fish species collected from the river. Heavy metal accumulation was also studied in other tissues such as skin, gills, liver and kidneys of two fish species i.e., Clupisoma naziri and Mastacembelus armatus. C...
Chiping Kong1,2, Yongheng Wu1,2, Shangling Lou1, Benping Chen3, Simon D. Dowell4 and Yiqiang Fu1*
...dually distinctive vocalisations are thought to be more pronounced allowing individual recognition based on songs. In this study, we explore individual identification in the songs of male Emei Shan Liocichla, a vulnerable babbler that inhabits dense vegetation. We recorded territorial songs of male Emei Shan Liocichla from Laojunshan National Nature Reserve during the breeding season of 2016, in southern Sichuan, China. We used three methods to compare the aco...

Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2 

...PC (3.21 mg GAE/ 100g of sample) as compared to control where POD (1.13 U/min g protein), SOD (1.46 U/ min g protein), and TPC (1.14 mg GAE/ 100g of sample). Amount of hydrogen peroxide (H2O2), the signaling molecule was also significantly increased (30.33 mmol/ mg FW) and catalase (CAT) was higher (91 U/min g protein) in fruit treated with ascorbic acid as compared to control (22.67 U/min g protein) treatment. These results...

Shazia Akhtar1, Abdul Samad1*, Afshan Gohar1, Muhammad Munir Shahid2, Muhammad Ishtiaq1, Arslan Sarwer1, Adnan Khan1 and Karam Ahad1 

...zed by using SPSS 21 and sample collection points were presented on map by using software Arc GIS. The response of interviewers was high which indicates their intentions to facilitate the present study. It was found that 12 varieties of peachs are being grown in the selected area. A total 28 pesticides were found which are being used on peach trees. It was noted during the survey that 98.6% respondents were unaware when to apply pesticides and they apply them...

Noorullah Khan1*, Farrukh Siyar Hamid1, Fayaz Ahmad1, Sabaz Ali Khan2, Imtiaz Ahmed1, Muhammad Abbas Khan1, Shamsul Islam1, Abdul Waheed1, Basharat Hussain Shah1 and Hussain Shah

...ifruit (Actinidia deliciosa) cuttings is very low and can be enhanced to a sufficient extent by treating cuttings with rhizogenic hormones. The present experiment was designed with the objectives to find the optimal concentration of IBA for better rooting and survival rate of seedlings from kiwi cuttings. The experiment was laid out according to randomized complete block design with three replications and 20 four budded branch cuttings of “Hayward”...

Fayyaz Hussain*, Raza Ullah Khan, Asim Hayat, Humair Ahmed and Ahmad Khan 

Hu Liu1,2, Qifang Yu1,2, Xiaopeng Tang1,2, Chengkun Fang1,2, Sijia Chen1,2 and Rejun Fang1,2,*
...ntrol group were fed a basal diet without adding exogenous selenium source, and the hens in other four groups were fed basal diets supplemented with SY and SS containing 0.3 ppm and 0.5 ppm, respectively. Pre-trial period lasted 7 d and experimental period lasted 42 d. The results showed that: compared with the control diets, 1) there was significant difference in average daily feed intake, and feed conversion rate (P < 0...
Mubasher Ahmad Malik1, Samina Jam Nazeer Ahmad2, Muhammad Jalal Arif1 and Jam Nazeer Ahmad1,*
...ntrol measures which are safer to the environment and compatible with human health. The present study was conducted to investigate the insecticidal properties of native isolated Plutella xylostella granulovirus (PxGV) and Azadirachta indica (AZA) on mortality and development of P. xylostella under laboratory conditions. Both AZA and PxGV were applied alone and in integrated manners at LC20 and LC50 dose rates. The com...
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
...
...
Kadry A. El-Bakry1, Lamiaa E.M. Deef1*, Lotfy Z. Habbak1 and Samia S. El-Naeli2
...weeks and 8 weeks. Blood samples for biochemical analysis and liver tissues were collected and fixed in 10% formalin for histopathological studies. The present study showed that, PAT causes elevated alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and significantly increases serum level of malondialdehyde (MDA) but decrease the activity of superoxide dismutase (SOD) enzyme. On the other hand, in patuilin injected rats and treated ...
Muhammad Muneeb1, Muhammad Ayub1, Sher Bahadar Khan2,*, Amjad Sohail1, Farkhanda Jabeen1, Mumtaz Ali Khan3, Amjad Khan4, Kashif Prince3, Asghar Khan5 and Irshad Ahmad6
...n Tehkal Region (0%). On same Agar high percentage for Escherichia coli was observed in samples from Hashtnagri (40%) and on tryptose agar results were quite high for Salmonella as compared to E. coli. In physiochemical analysis high pH was observed in samples taken from Warsak Road (6.84) followed by Hashtnagri. High moisture conten...
Erum Iqbal*, Nasir Mehmood, Nasira Kazi and Shahina Fayyaz
...s were encountered. Soil sample analysis revealed two and a known plant parasitic nematode species viz., Paratylenchus manilkarii n. sp., P. sindhicus n. sp.,and Pratylenchus kralli Ryss, 1982 as a new record species. P. manilkarii n. sp., is characterized by the lateral field with four incisures; stylet 28-29 µm long; vulval lips protruding with vulval flap; lip region rounded or truncated without submedian lobes, tail ventra...
Umer Iqbal1,* and Tariq Mukhtar2
...ndazim and Propineb with same concentration exhibited 66.67 and 63.33% plant survival respectively. Copper oxychloride proved to be the least effective. 
...

Muhammad Akbar Ali Shah1, Gamze Özel2, Christophe Chesneau3, Muhammad Mohsin4, Farrukh Jamal5* and Muhammad Faheem Bhatti6 

... 31 villages selected as samples randomly. Time series data collected from Crop Reporting Service (CRS) for the period of last 10 years (2005-2014) containing 516 cases and 14,964 observations. Two different multiple linear regression (MLR) models are applied to study the relationship between the yield of rice (dependent variable) and the various factors (independent variables) which are affecting the rice crop production. The Model I is based on monthly avera...

Ghulam Murtaza1*, Ghulam Sarwar1, Noor-Us-Sabah1, Mukkram Ali Tahir1, Fakhar Mujeeb2, Sher Muhammad3, Muhammad Zeeshan Manzoor1 and Ayesha Zafar1  

...gn: justify;">The use of saline irrigation water leads to the buildup of salts in root zone, resulting in decreased yield and deterioration in soil resource. Continues use of organic manures, help in the reduction of salt concentration in the root zone. Organic matter was used to mitigate the negative effects of saline water irrigation using sorghum as t...

Muhammad Sohail Memon1*, Khadim Ullah1, Altaf Ali Siyal1,2, Naimatullah Leghari1, Ahmed Ali Tagar1, Khalil Ahmed Ibupoto1, Syed Tahir Ata-ul-karim3, Muhammad Tahir4 and Noreena Memon1 

...due to its capability of saving massive amounts of irrigation water. Knowing the importance of raised bed-furrow irrigation (RBFI) in water conservation and salt distribution in profile with three testing treatments viz. T1 with raised bed size = 0.6 m, T2 with raised bed size = 0.8 m and T3 with raised bed size = 1.0 m. The soil samples were collected from depth desired depth to analysis ...

Muhammad Qaisar Nawaz*, Khalil Ahmed, Ghulam Qadir, Muhammad Rizwan, Muhammad Faisal Nawaz and Muhammad Sarfraz 

...duction under moderately salt-affected soils. The experimental design was split-plot with sowing methods (bed and ridge) in the main plot and nitrogen doses (40, 60, 80 and 100 kg ha-1) as sub plot. Treatments were replicated three times with sub plot size of 4 m × 6 m. Measurements included were: number of plant/m-2, number of leaves/plants, root length, bulb diameter, forage yield and total bulb yield. Results revealed that all the studied parameters w...

Basharat Hussain Shah*, Farrukh Siyar Hamid, Shams ul Islam, Naveed Ahmed, Fayaz Ahmad, Noorullah Khan and Qamar uz Zaman. 

Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*

...rimp’s lethality assay. All the extracts showed significant biological activity against Gram positive, Gram negative bacteria and selected fungal strains. Acetone, chloroform and methanolic extracts showed maximum activity i-e 39mm, 44mm and 35mm followed by ethyl acetate 27mm and n-hexane 26mm against microorganisms studied. Standard antibiotics were used as a positive control for bacteria and fungi respectively. The cytotoxic as

Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein

...is study, 100 FMDV field samples were collected in 2018 and early2019 from governorates in Egypt. Molecular and genetic characterization indicates that serotype SAT2 is widespread (in 96 cases), while only four cases of Serotype O were detected. Phylogenetic analysis of the study sequences indicates that the circulating FMDV serotype SA

Bamigboye Olutoyin Omolara1, Fapohunda Funmilayo Omotoyosi1, Oladipo Elijah Kolawole1,2, Oni Mattthew Olujenyo1, Ajibade Oluwatosin Akinola1, Kaka Mary Oluwatosin1* 

...h HIV. Ninety-three sera samples of HIV positive individuals were collected by Ladoke Akintola University of Technology (LAUTECH) Teaching Hospital- HIV/AIDS Supporting and Counselling Unit, Ogbomoso. The collected samples were analyzed for enumeration of CD4+ cells and presence of HBV. The selected three HBV serological markers were HBsAg, HBeAg and HBcAb while using a third generation ELI
Monica Paula Marin1, Elena Narcisa Pogurschi1*, Iuliana Marin2 and Carmen Georgeta Nicolae1
...g the concentration of unsaturated acids (oleic, linoleic) and immunoglobulins in colostrum, as well as reducing the diarrheal gastric disease of cattle. By adding zeolite in the cow ration during the precalving period has determined a significant increase in blood serum calcium during the last week of precalving period, as well as in the calving and postcalving periods, having as consequence fewer recorded milk fever cases. The results obtained can convince t...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*

Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5

...method. All ICT positive samples were further confirmed through ELISA. Individuals 25(8.2%) were found positive for both hepatitis B surface Antigen (HBsAg) and Anti hepatitis C antibody (Anti-HCV antibody) after initial screening with no coinfection of both diseases. Out of 25 total infected individuals, 05(1.6%) were found HBsAg positive and 20(6.6%) were found anti-HCV positive. All the...
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2,  Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1Aneela Zameer Durrani4 and Muhammad Usman4*
..., polypnea, cough, and nasal discharges and caused by Mycoplasma mycoides subsp. mycoides. This study was carried out in abattoirs of Lahore, Kasur and Jhang districts of Punjab, Pakistan. Different tissue samples were collected from 560 cattle showing respiratory signs at slaughter. Lung samples were used for detection of CBPP through PCR targeting 16S ribosomal RNA. Further...
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
...ysis the effect of immunisation of somatostatin (SS) DNA vaccine in maternal animals on serum related hormones in offspring. In this study, ten ewes with two newborn lambs were randomly divided into SS DNA vaccine group (Group T) and empty vector group (Group C), and these vaccines were given orally into the ewes at weeks 0, 3 and 6 of the research. Blood samples of ewes or offspring lambs were collected at week 6 or 9 of la...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
...biological (water, soil) samples were collected. Soil was collected from different depths (6, 12, 18 inches) to study deposition patterns of metals. Samples were chemically digested and finally analysed by Flame Atomic Absorption Spectrometery (FAAS) for four heavy metals such as Pb, Cd, Zn and Cu. The results showed all the metals were in safe range in feed samples, a very negligible amou...
Abdul Samad1, Raheela Asmat2, Muhammad Naeem1, Hamida Ali3, Mohammad Zahid Mustafa1, Ferhat Abbas1, Jannat Raza1,3 and Muhammad Tauseef Asmat1*

 

... present study, 800 food samples consisting of beef and chicken meat, milk, salads and fresh vegetables were collected from retail markets of Quetta, Pakistan. Multiplex PCR was employed to identify the prevalent species and genes associated with virulence. Only 10 (1.25%) samples were found contaminated with Listeria monocytogenes. Milk samples w...

Muhammad Israr1*, Helen Ross2, Shakeel Ahmad3, Nafess Ahmad4 and Urooba Pervaiz5

...ure development are necessary to overcome the situation and improve the likelihood of achieving the targets of the SDGs. 

...

Shahzad Khan1*, Inayatullah Jan1 and Syed Fahad Shah2 

...villages of District Charsadda Khyber Pakhtunkhwa, Pakistan, were purposely selected for the study. A total of 30 respondents were interviewed with the help of a pre-tested questionnaire. The findings of the study reveal that skillful and experienced tenants were given preference by the landlord. But due to non-availability of extension department and its trainings, illiterate and untrained tenants are involved in agricultural practices. The physical inputs an...
Yanyan Yu1,2,3, Yizhong Hu1, Qipeng Zhang1, Rongquan Zheng1,4,*, Bing Shen1, Shenshen Kong1 and Ke Li1
...e genus Quasipaa spinosa detected the phonotaxis of the female frog for the calls from different lineages. In this work, we used a single-stimulus design to analyse phonotactic response to stimulus and subsequently estimated the values of female preference functions for call duration and call rate, and recorded and analysed the properties of 756 advertisement calls from 40 males at five reproductive stages and conducted single-stimulus phonotaxis experi...
Haroon1,2, Yu-Feng Meng1, Zahid Khan1, Farzana Perveen2, Muhammad Ather Rafi3, Sayed Waqar Shah4, Xiao-Hong Su1 and Lianxi Xing1,*
...s explored in Tangi, Charsadda, Khyber Pakhtunkhwa, Pakistan. A total of 506 specimens collected out of which 252/506 were belonging to family Nymphalidae, 217/506 from family Pieridae, family Papilionidae represented only 37/506 individuals. With their abundance Danauas chrysippus (F130, F314 and D3 24.11), followed by Junonia orytha (F121, F310 and D3 11.86), while Papilio polytes, Junonia hierta and Euthalia garuda showed ...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...ure black and brown goat samples, which were mutated from tryptophan to cysteine. The results of this study have important reference significance for the future correlation analysis between MC1R gene and Nubian goat wool color traits and the color genetic mechanism of Nubian goats.
...

Anand Kushwaha, Amit Kumar, Aparna Madhavan, Durga Goswami, Golmei Poulinlu and Gnanavel Venkatesan* 

...otein based diagnostic assays namely ELISA is safer and robust to handle large sample size and also to minimize labor/time. However, the genus Capripoxvirus encodes putative 147 proteins in their genome, among which some of them are reported as potential immunogenic candidate genes. Selection and use of such candidate ...

Majid Khan, Naveed Jehan*, Muhammad Zahoor and Nadia Naz 

...016 in Tehsil Tangi, Charsadda District, Khyber Pakhtunkhwa. The aim was to analyse the poverty status of the Virginia Tobacco growers in the study area with Poverty Gap Index (PGI) method. The primary data was collected through structured questionnaire from 159 farmers. Monthly farmers’ income was calculated from the total crop and non-crop income. The results revealed that the poverty ratio and Poverty Gap index were 8.3 percent and 0.016 percent for t...

Ajmalud Din1*, Munir Ahmad2, Fahad Masoud Watto2, Sheraz Ahmed1, Imtiaz Ali1 and Muhammad Kausar Nawaz Shah

...genized mixture of loam, sand and compost soil. Two factorial Completely Randomized Design was followed with three replications and two treatments. The experiment was conducted during 2015-16 in the experimental site of the Department of Plant Breeding and Genetics, PMAS Arid Agriculture University Rawalpindi, Pakistan. Data were recorded on drought-related physiological traits viz. proline content, osmotic adjustment, excised leaf water retention (ELWR), rela...

Tariq Mahmood Khalil1,2*, Muhammad Ajmal2, Taj Ali Khan2, Zia Ul Haq2, Muhammad Shahzad Khattak2 and Abdul Malik2 

...ters marked S1-S9 having sample size of 27 were tested on the hydraulic bench. When evaluated for compliance with manufacturer claim for being marked as PC and Non-PC, all the emitters passed the test except for S1. The statistical uniformity of S9 ranked highest (96.62%) compared to other emitter types, while S2 ranked lowest (87.99%). When appraised for discharge variation under variable head, S3 discharge variation was not significant (2%) whereas S8 had th...

Muhammad Ramiz Murtaza1, Tahir Mehmood2*, Aziz Ahmad2 and Uzma Arshad Mughal3 

...ffar Garh. Simple random sampling technique was used to select 110 mango growers from 4 union councils of tehsil Ali Pur. To scrutinize the data double log model was renovated from Cobb-Dougles production function. Results showed that Benefit Cost Ratio (BCR) with and without intercropping was 1.72 and 2.48 respectively. Nearly 64 percent of the factors analyzed were affecting mango production and adjusted R2 was at 0.62. The whole goodness of fit model was en...

Saleem Ashraf1, Raheel Saqib2*, Zakaria Yousuf Hassan3, Muhammad Luqman4 and Abdur Rehman5  

Rakhshanda Kousar1, Muhammad Sohail Amjad Makhdum2, Raza Ullah1, Aqeela Saghir3, Sohaib Usman3* and Tahira Sadaf

...mers of rural area of Faisalabad. This study calculated the extent of land fragmentation by using Simpson index. Production function was employed to estimate the impact of land fragmentation on the crop productivity. The results suggested that higher the land fragmentation of the farms, negative is the impact on the productivity. The findings of the study have important implication for formulating of efficient land use policy. 

...

Gulfam Hassan1*, Ijaz Ashraf2, Muhammad Qavi Irshad3, Shafiq-ur-Rehman Zia2 and Muhammad Idrees

...n peri-urban areas of Faisalabad, Pakistan. Two hundred and eight vegetable growers were interviewed using a validated and reliable interview schedule. Findings reveal that cooperation of training staff was ranked first followed by use of simple and familiar words during training sessions and provision of timely information in case of strengths of training programs. While looking into weaknesses of trainings, vegetable growers disclosed the unavailability of l...

Yousaf Ali1, Muhammad Zamin1*, Ibadullah Jan1, Shahen Shah2, Muhammad Mazhar Hussain3, Fazli Rabbi4 and Muhammad Amin

...mpost, mixture, clay and sand) and six tomato genotypes (Nadir, Money Maker, Pakit, Nagina, Roma and Rio grande) with three replications. The results revealed that the combination of compost and mixture has significant effect on shoot height, number of leaves, stem and root length, however stem diameter was significantly affected by compost and non significantly affected by genotypes. As far as the genotypes performance is concerned, genotype money maker showe...

Anwar Ali1*, Muhammad Irfan Ashraf2, Saeed Gulzar2 and Muhammad Akmal2

...cted through destructive sampling of 32 sample trees growing in subtropical pine forests of Hazara Forest Region, Khyber Pakhtunkhwa (KP), Pakistan. Diameter at Breast Height (DBH) of the selected sample trees ranged from (12-93 cm) and height (H) varied between 9.50-40.10 m. Allometric equation was developed through testing different allometric models. The best fit model was obtained thro...

Nosheen Noor Elahi1, Muhammad Shafique1, Muhammad Imtiaz2, Umer Farooq3* and Muhammad Rashid1 

...liminating the effect of salinity. Cicer arietinum L. is an important legume crop having high quantity of proteins. In the present study, four varieties (CM44, CM91, CM98 and CM2000) were grown in the presence and absence of PGPR inoculated media and nitrogen in different salinity levels (20, 50, 100, 200 and 300mM NaCl). The biomass production of the varieties CM91 and CM98 increased at 20-100mM NaCl concentrations but dras...

Faisal Azeem Abbassi1, Atta Ullah2, Muhammad Saim Hashmi1, Khadim Hussain1* and Nasim Akhter1 

...ected through convenient sampling. Endowment of livelihood assets was calculated by constructing asset indices. Asset weights were obtained using Principal Component Analysis (PCA). Assets indices revealed that district has performed best on physical capital (index value 0.63) and human capital (index value 0.59) dimensions. It was found that foreign remittance is the most popular livelihood strategy (29% of households’ main source of income) in the dist...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
...try industry. As a universal innate immune gene, Myeloid differentiation primary response gene 88 (MyD88) can activate the nuclear factor-κB (NF-κB) pathway and regulate downstream gene expression. Single nucleotide polymorphisms (SNPs) in the coding regions (CDS) of the MyD88 gene have also been reported to be associated with inter-subject differences in responses to Salmonella Pullorum infection in chickens. However, w...
Muhammad Asif Gondal, Qazal Waheed, Sana Tariq, Waseem Haider, Aisha Khan, Qudsia Rasib and Haroon Ahmed*
...aridae. Freshwater snail samples were collected from Islamabad and Rawalpindi in screw capped sterilized bottles from various localities during the months of July 2017 to December 2017. Physical parameters affect the presence of snails e.g. alkalinity of the soil indicates the survival of more number of species. A significant correlation was found between diversity and equitability (Pearson correlation value=0.961, p-value=0.00). Higher the electrical c...
Chuntao Li1, Hua Liu2, Jianqing Zhang1*, Jiaqiang Zhang1, Luming Dai1, Zhihuan Zhao1,Lizhou Fang1, Ling Liu1, Jingkui Shu1 and Jiagang Feng1
...NAs and lncRNAs in blood samples from COPD patients (n=3) and healthy controls (n=3) with smoking history by human microarray analysis. Then, we selected lncRNAs and their associated mRNAs which were both differential expression in COPD vs healthy smokers, for quantitative reverse transcription PCR (qRT-PCR). A total of 3,359 lncRNAs revealed by microarray analysishave been differentially expressed between the two groups, 1,995 lncRNAs identified to be up-regu...
Dilara Abbas Bukhari*, Mehwish Faheem, Shamoona Arshad and Khalid P. Lone
...ale and female mice were sacrificed and kidneys were taken out. Biochemical parameters e.g., lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH) were determined to evaluate oxidative stress while urea and creatinine, was used to evaluate kidney function. It was found that there was an increase in weight (p<0.05) of both males and female mice fed with diet containing ghee. Kidney LPO and CAT were significantly...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
...he experiment, the liver samples were weighed and histopathologically evaluated using a knodell score to assess portal hypertension and liver damage. Animals fed with commercial chicken meat and feed for six weeks showed development of inflammation, necrosis, apoptosis and cirrhosis on histopathological examination of the liver, and had raised plasma TNF-α and AFP-levels. This study shows that damage to liver cells is caused by consumption of commercial ...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...oacal swabs and 15 water samples were gathered from different localities in Giza and Cairo Governorates. An additional 50 stool specimens were collected from individuals in contact with the examined chickens. Eleven Campylobacter isolates were recovered through bacteriological examination. Campylobacter spp. were identified by polymerase chain reaction (PCR) as C. jejuni (63.6 %) and C. coli (36.4 %) through the detection of the ...
Bhandari Jyoti1,2, Yasen Shali3, Zhang Zehua4, Yao Xiaoho5, Deng Jiang3, Zhang Yaling5, Wang Cuiling5, Li Yang5, Lei Xueping5, Wang Wenfeng5, Mohammad Farooque Hassan6 and Li Can1,*
...e species traits is necessary. In this study, the population dynamics, life history and spatial distribution pattern of L. m. tibetensis Chen were explored on meadow and farmland of the Tibetan plateau during 2012. The first objective of this study was to investigate the population dynamic and life history of this pest on their outbreak sites. The second objective was to analyze the spatial distribution pattern of L. m. tibetensis Chenon both mea...
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...d radix sections. The dorsal and ventral surfaces of the tongue were covered by a keratinised stratified squamous epithelium. Lamina propria and submucosal layers were distinguishable underneath the epitelium. SEM observations of surface of the radix linguae revealed conical papillae and tongue caudally directed lingual papillae. Thus, in this study, the anatomy and histology of the tongue of the guinea fowl tongue were exam...
Gasem Mohammad Abu-Taweel
... number of fights, nasonasal, nasogenital and rears were decreased significantly. The latency to first bite was significantly increased, however the number of bites was decreased dramatically when compared with control. Motor activity and grip strength data were decreased significantly in exposed offsping. Interestingly, blood parameters like packed cell volume, red blood cell count, hemoglobin content, platelets and white blood cells were significantly reduce...
Nafeesa Batool1*, Javed Iqbal Qazi1, Nida Aziz1, Ali Hussain2 and Syed Zawwar Hussain Shah3
... can conclude that BMP assays are extremely useful to determine the amount of bio-methane and bio-degradability of the organic substrates and would facilitate in the preliminary selection systems for the field scale applications especially in framework of developing countries.
...
Elmas Ulutaş1,*, Abdullah Eryavuz2, Aziz Bülbül2, Abdur Rahman3,*, İsmail Küçükkurt4 and Cangir Uyarlar5
...rol group (C) fed with basal diet containing 31.76 ppm Zn, experimental group 1 fed with basal diet supplemented with 500 ppm Zn, experimental group 2 fed with basal diet supplemented with 750 ppm Zn and experimental group 3 fed with basal diet supplemented with 1000 ppm zinc sulphate. The investigation was started after 15 days of adaptation period and ...
Jam Nazeer Ahmad1,2*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2*Mubasher Ahmad Malik1, Mujahid Manzoor1, Muhammad Tahir2,  Zubair Aslam4, Sumaira Maqsood5, Ishita Ahuja3 and Atle M. Bones3
...tera litura and Spinosad (0.01 ppm) against 3rd and 4th instar larvae collected from three different geographical areas of Punjab (Pakistan). A difference in larval mortality, pupation, adult emergence and egg ecluson was observed. The higher but sub-lethal dose rate of NPV with Spinosad exhibited synergistic interaction, while the rest of the combinations were found additive in all the tested popul...

Dilawar Khan1*, Arif Ullah2, Zainab Bibi1, Ihsan Ullah1, Muhammad Zulfiqar4 and Zafir Ullah Khan

...sted period is 61.37 thousand hectares each year. Area of guava shows a static trend throughout the forecasted period. The forecasted production of guava is 502.14 thousand tonnes in the year 2029-30. Guava production can be increased using improved guava cultivars, improved system of irrigation and adequate cultural practices. Strategy should be adopted to bring more barren land under guava cultivation. This would help to i...

Saba1, Shahnaz Akhtar1, Waqar Khalid2* and Saifullah Khan

... Pakistan. A multi-stage sampling approach was adopted to choose a random sample of 110 women farmers from the union council of village Shewa, and a well-structured questionnaire was designed to accumulate the primary dataset from rural women through face to face interviews. A women’s participation index was designed to compute the degree of women’s involvement, and a multiple linear regression model was proposed...

Iqbal Javed1, Abdur Rehman2*, Farhana Khaliq1, Amar Razzaq3, Mudassar Yasin4, Ghulam Mustafa5, Allah Bakhsh6 and Raheel Saqib7 

...ty standards and provide satisfactory returns on the resources which is used in production of a commodity. Nominal protection coefficient (NPC) is projected for the time period of 2003 to 2016 for using as a dependent variable in the current study. To estimate the impact of different macroeconomic variables on basmati export competitiveness panel data set is used following Park’s Feasible Generalized Least Square (FGLS). According to the results of the s...

Mohammad Aquil Siddiqui1*, Muhammad Tahir Khan1, Ghulam Shah Nizamani1, Shafquat Yasmeen1, Imtiaz Ahmed Khan1, Abdullah Khatri1 and Nighat Seema Soomro2 

...the plants as an indispensable tool for crop improvement. This field study was initiated to evaluate three potential mutant lines of lentil against their parent (M-85) and two check varieties (NIA-Masoor-05 and NIA-Masoor-16). The pooled data of the crop, after two years of evaluation, indicated the earliest maturity in the mutant AEL-40/30 (92.0 days). Plant height was observed to be low in all the studied mutant lines (AEL-40/30, AEL-13/30 and AEL-12/30) aga...
Nihat Yeşilayer*
...t (WBT), farmed Atlantic salmon (FAS) and farmed Rainbow trout (FRT). The study was conducted on three fish species to investigate the relationship between total carotenoid concentration (TCC) and flesh colour, determined by visual and colorimetric methods, of three fish species. Linear relationships were observed between CIELAB colour measurements (lightness-L*, redness-a*, yellowness-b*, hue-H°ab and chroma-Cab), DSM SalmoFan card scores and TCCs....
Nadeem Munawar1,2*, Tariq Mahmood2, Paula Rivadeneira1, Ali Akhter2 and Saqib Mehmood2
Tian-Yi Zhao1, Zi-Qing Liu2, Shu-Fei Ma3, Bo-Yang1, Fan-Fan Guo1 and Ming-Hua Duan1*

 

...)-induced AD. In vitro assays (MTT and flow cytometry) were applied to detect the effect of biatractylolide on PC12 cell proliferation, growth inhibition rate and apoptosis. In order to assess the spatial learning and memory abilities of AD rats, Morris water maze model was applied in vivo, and the activity of the NF-κB signaling pathway and concentrations of TNF-α, IL-6, and IL-1β were measured. The results demonstrated that biatractylolide c...
Su-Hu Duan, Liu-An Li*, Zai-Qiang Li, Meng-Ran Qin, Zhen-Zhen Fan, Qian Wang, Ke-Yan Zhang, Zhong-Mou Zhang, Bai-Liang Yang and Tian-Ming Jin
...dicate that: In the test samples, totally 7 types of SFA, 7 types of MUFA, and 8 types of PUFA were detected. Specifically, no significant difference was found in the content of C14:0~C18:0 (P>0.05). The content of C16:0 in the yolk of both native eggs and ordinary eggs was high; in the yolk of native eggs of the three brands, the content of C14:1 and C16:1 was both significantly higher than that of ordinary eggs (P<0.05); the content of C1...
Ju Zhang1,2, Dong-Sheng Jia3, Shi-Hao Zhao1,2, Xiao-Liang Xie3, Hong Chen4 and Hai-Feng Li4,*

 

...ipofuscin fluorescence assays in wild-type C. elegans N2. Calorie restriction pathway was detected by pharyngeal pumping rate assay and lifespan examination in sir-2.1 mutant nematodes. The antioxidant activity of HGQ was investigated by determining reactive oxygen species (ROS) level, paraquat-stress survival rate, malondialdehyde (MDA) content as well as superoxide dismutase (SOD), catalase (CAT) and glutathi...

Haidar Ali1*, Malik Muhammad Shafi1, Himayatullah Khan1 and Hamra Haidar

...as interview schedule. A sample of 298 small farm households were selected using random sampling technique for this study. Analysis showed that small farmers of developed villages perform more off-farm employment than small farmers of underdeveloped villages. This could be associated to the developed means of transport and communications, better education facilities, market facilities as well as availability of off-farm jobs...

Sanaullah1*, Urooba Pervaiz1, Shahid Ali2, Mohammad Fayaz2 and Aftab Khan

...as (FATA) of Pakistan. A sample of 166 respondents was selected through multistage stratified random sampling technique. Data were collected with the help of a pre-tested and validated well-structured interview schedule. Analysis was carried through multiple regression and independent sample t-test. Results indicated that extension visits, involvement in agriculture, adoption of recommende...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
... virus and infectious bursal disease. Relative lymphoid organ weights were recorded on day 42. Hemagglutination inhibition (HI) assay was performed against Newcastle disease virus on a weekly basis, whilst a hemagglutination (HA) assay was performed to determine immunity against total sheep red blood cell (SRBC) , mercaptoethanol-2 resistant (IgG) and mercaptoethanol-2 sensitive (IgM) anti...
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...cterial diversity of the saline lake, situated in the salt range, Punjab, Pakistan and to screen them for antagonistic activities against multi drug resistant pathogens and to characterize the bioactive metabolites produced by them. A total of seventy strains of actinomycetes were isolated from lake samples, out of them only forty bioactive strains were selected for further characterizatio...
Sevil Demirci*, Aydın Demirci and Emrah Şimşek
...l, 1986). A total of 587 samples were collected from the eastern Mediterranean coast of Turkey between February 2012 and January 2013. Total length and total weight ranged from 7.7 to 21.0 cm and from 6.90 and 150.99 g, respectively. Length-weight relationship parameters were calculated for all individuals as a= 0.0106 and b= 3.09, and isometric growth was determined. The population constituted five age-classes (0–IV years). Four growth mod...
Muhammad Faheem Akbar1,*, Muhammad Abdul Haq2, Imtiaz Ahmad1, Vasileva Viliana3 and Amjad Sultan1
...convectional, while spinosad and biosal (neem compound) as bio pesticides, and were sprayed at the rates of 49.4, 642.2, 988, 35.5 and 158 g a.i. ha-1 respectively. The pesticide residues were analyzed after 0, 1, 3 and 7 days of application in the leaves and cauliflower curd using high performance liquid chromatography. First order degradation kinetics was fitted on this data and degradation rate constants and ha...

Stanley Uchenna Onwudike* and Vivian Chizoba Edoziem 

...s esculentus) on a loamy sand, typic haplustult soil. The work was sited at the research farm of the University during 2016 planting season and the region lies within the latitude of 5° 38¹N and longitude 6° 97¹ E. At 10 weeks after planting, both the rhizosphere soil (soils within 2 mm region of the test crops) and bulk soil or control (soils away from the root zone) from the experimental plots that were planted with bean, groundnut and okra...

Mahfooz Khan* and Himayatullah Khan 

...olds using simple random sampling. The sample size constituted 5 percent of total farm households in selected villages of district Peshawar. A multinomial logistic regression model was used for choice of alternative energy sources for cooking purpose. Results of the study showed that income of farm households, education level of household head and cooking cost had significant effect on the use of electricity, firewood and na...
Abdul Kabir¹, Amjad Hussian Mirani¹, Jam Kashif1, Shumaila Manzoor2, Abdullah Iqbal3*, Ihsan Ullah Khan4 and Muhammad Abubakar2
...PK. A total of 246 serum samples were randomly collected from three age groups (<1 year, 1-2 year and >2 years) of sheep and goats. Competitive ELISA was performed to evaluate sero-prevalence of PPR in goats and sheep. The swab and tissue samples were collected from the surrounding areas from suspected sheep and goats to confirm the PPRV. Out of to...
Jun Yan Bai*, Hong Deng Fan, You Bing Yang, Xu Wang, Heng Cao, Xue Yan Fu and Yu Qin Wang
...both of which coding the same aa, alanine, indicating C155T was a synonymous mutation site. The C624T mutation site of GHSR-4 gene led to a code change from CTT to CCT, and the corresponding aa changed from leucine to proline, which was a missense mutation site.
...
Jun Yan Bai*, Xiao Hong Wu, Shuai Yang, You Zhi Pang, Heng Cao, Hong Deng Fan, Xue Yan Fu, Kun Peng Shi and Xiao Ning Lu
Mounir R. Abi-Said1, Mohammad Al Zein2, Mohammad A. Abu Baker3 and Zuhair S. Amr3,4*
Kadir Seyhan1,*, Nimet Selda Başçinar2, Nadir Başçinar3 and Umar Khan3
...ir stomach contents were sampled at predetermined postprandial times, and were dried at 60° C to constant weight. The course of GE in both sizes of S. fontinalis fry was best described by the square root model. The relationship between GER and body mass was then determined by a power function of fry mass that can be summarized by the equation (g h-1), where St is current stomach mass (g), M is fry mass (g), a...
Muhammad Younis Laghari1,2, Zixia Zhao1, Yiwen Gong3, Punhal Lashari1,2, Shangqi Li1, Jian Xu1 , Ateeque Rahman Khooharo4, Jiongtang Li1,* and Yan Zhang1*
...s developed and then 120 samples were used as templates for PCR validation. The specific marker will facilitate the assessment of genetic diversity and selective breeding programs for Hainan common carp.
...

Muhammad Rasheed*, Tayyaba Naseer, Asma Hassan, Fayaz ul Hassan, Rifat Hayat, Ghulam Jilani, Samman Gul Vaseer and Muhammad Bilal Ali 

... the rhizosphere is necessary that improves the nodulation, N2 fixation and growth of legume crops. The experiment was conducted to access the effect of isolated bacteria from nodules on growth and nodulation of the lentil cultivars. Different cultivars were used in this experiment; Markaz 2009, Masoor 2009 and NIA 2005 treatments were used viz BS0 (control), (Pseudomonas stutzeri), BS2 (Lysinibacillus pakistanensis) and BS3 (Pseudomonas stutzeri + P2O5@40kg/h...
Rafia Sadaf1, Ishrat Younus2*, Sidra Maqbool2, Talha Bin Fayyaz3Sarah Jameel Khan1, Sidra Siddique1 and Rida Fatima1
...font-size: small;">Pulsatilla nigricans Linn has traditionally been used in various disorders related to both male and female and proves a great remedy for ovaralgia, ovaritis, epididymitis, orchitis nervousness, sadness, mild restlessness and mental disorder. This investigation is designed to evaluate acute toxicity and anti-anxiety activity of ethanol extract of Pulsatilla nigr...
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
.... pneumoniae, P. aeruginosa, E. coli and Acinetobacter Species was 26%, 52%, 27% and 5% respectively. Prevalence of blaNDM-1 gene was 51% identified in 14 strains of P. aeruginosa, 2 strains of A. Baumanni, 29 strains of E. coli and 18 strains of K. pneumoniae. In conclusion, high prevalence of blaNDM-1 in our strains is a serious concern. A careful use of carbapenems is important to prevent the spread of these organisms.
Mubashra Salim1, Omeira Ibrahim1, Hugo Vilhena2,3,4, Carla Maia5, André Pereira5, Maria Shahzeen1, Shabana Kalsoom1, Asim K. Mahmood6 and Furhan Iqbal1*
...utum in feline blood samples collected from various pet clinics in Pakistan, by Polymerase Chain Reaction (PCR), using 16S rDNA as the target sequence. Clinical and epidemiological data was collected in all animals included in the study. M. haemofelis and C. Mycoplasma haemominutum DNA was detected by PCR respectively in 6.8% (10/148) and in 18.2% (27/148) of cat blood samples. Of these, two animals were co...
Veli Sel1, Isa Yilmaz2,* and Mete Yanar3
...ose, a total of 637 milk samples gathered from 91 animals raised in 54 farms were analyzed and statistically evaluated. The age and education level of the farmers and the effect of the milker on the SCC were found to be significant (P <0.001). SCC value (65.930 ± 2.484 cells/ml) was found to be the lowest when the milker is the person from the household. The udder cleaning before milking was a significant factor (P <0.001), SCC was 66.330±2...
Tasbihullah1, Sadeeq ur Rahman2,*, Tariq Ali3 , Umer Saddique1, Shakoor Ahmad1, Muhammad Shafiq4, Sultan Ayaz2, Hamayun Khan1, Ijaz Ahmad1 Asadullah2, Raheela Taj5, Hafiz Nidaullah6 and Alamgir Khan7
... table eggs and 50 stool samples from human were analyzed. Results showed that out of 80 E. coli isolates recovered from eggs, 20 (25%) were found to be ESBL-producers, while, of the 17 human-isolates, 4 (23.5%) were ESBL producers. PCR revealed that blaCTXM (blaCTXM-1=15 and blaCTXM-9=4) was carried by all 22 (91.6%) ESBL-producers with additional blaSHV2 (n=12) and bla...
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2Maqsood Akhtar3 and Muhammad Shahzad4
... animal excluded at milk sampling from group-3 due to unavoidable circumstances. Oxytocin was administered to each buffalo twice daily @10IU and 30IU into group-2 and group-3 respectively, whereas, saline into group-1 within one week of post calving for 48 days. Ultrasonography was performed twice a week to monitor involution changes while milk composition analysis was done once a week. Results depicted that initially, anech...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...sin receptor blocker, Valsartan, were evaluated in the treatment of chronic renal failure (CRF) with and without the presence of N-acetylcysteine (NAC). The renal mass of Wistar albino rats was reduced at a rate of 5/6. Captopril, Valsartan and NAC were applied intra-peritoneal alone or in combination. Blood pressure and heart rate were monitored at weekly intervals over a period of six weeks. Serum creatinine, blood urea ni...
Jiao Qin,Qian-Qian Su and Quan-Sheng Liu* 
...n the uteri regardless dosage and time. PRL in female rats treated by 50 µg/kg cabergoline significantly was decreased by 56% at day 7 compared with the control. However, estradiol (E2)in rats treated with 50 µg/kg cabergoline was higher than that in control group at day 24, and progesterone (P) secretion was not remarkably influenced by cabergoline. Moreover, the duration of investigation toward normal females in the 50 µg/kg grou...

Asmaa A. Darwish 

...cal alterations, which disappoint our therapeutic programs and frustrate our expectation for curing. This work aimed to assess the most important clinicopathological alterations related to sheep pneumonia, arthritis and enteritis and spot the light on MMP-2 and MMP-9 diagnostic and prognostic accuracy in these conditions. 80 barki lambs were divided into four groups control group (CG), pneumonic group (PG), arthritic group (AG) and enteric group (EG). Blood

Kenneth Nnamdi Anueyiagu*, James Jingmang Kopmut, Chrysantus Achi Lagi and Kelly Nkem Okoh 

... determine the rate of nasal carriage of Methicillin Resistant Staphylococcus aureus (MRSA) among students in a college of animal health and production and livestock in the College Farm. 160 nasal swab samples taken from 130 students from three schools of the college and 30 livestock comprising sheep, goats, and cows b...
Anila Naz Soomro1*, Hiroshi Suzuki2, Sadaf Tabassum Qureshi3 and Wazir Ali Baloch1
...tyid shrimps Caridina sakishimensis and Caridina typus along the continuum of Urabaru stream. Study was carried out to clearly outline the life cycle of an endangered atyid C. sakishimensis and its co-existing species C. typus. Entire continuum of stream was divided into five stations (St.1, St. 2, St. 3, St. 4 and St. 5). Monthly sampling was carried out using ...
Daniel Cocan1, Vioara Mireşan1, Florentina Popescu1, Radu Constantinescu1, Aurelia Coroian1, Călin Laţiu1, Romulus Valeriu Flaviu Turcu3, Alexandru Ştefan Fărcăşanu3 and Cristian Martonos2,*
...lse sequence. In transversal sections, organs, blood vessels, and bone formations appear unchanged in shape, size, appearance and location. For the studied species, the venomous glands, together with the first radius of the pectoral and dorsal fins, as well as the muscular fascicles attached to the radius, are forming the venomous apparatus. Triangular positioning of pectoral and dorsal sp...
Zhi Yi Jin and Shao Ming Qin*
...ent analysis showed that safety factors (vegetation cover on both banks, distance to a mine factory and river width) were the three most important habitat factors, indicating that Scaly-sided Merganser had high requirements for shelter and safety. Twenty two species of fish belonging to eight families and three orders were identified in foraging areas of the Scaly-sided Merganser. We could identify only six fish species eate...
Syed Basit Rasheed*, Ahmad Yar, Farrah Zaidi and Qaisar Jamal
...m all three tehsils: Charsadda, Tangi and Shabqadar of district Charsadda. Ants were collected randomly from indoor (rooms, kitchen and washrooms) and outdoor (streets, crop fields, barren ground and grave yards) sites by using buccal aspirator, pitfall traps and hand pick method. A total of 19 species representing 11 genera and three subfamilies namely Formicinae, Myrmicinae and Dolichoderinae were identified during the pre...
Boxin Dou, Ying Liu*, Yumeng Liu, Lili Fan, Yongqiang Ma, and Yanguo Shi
...ively, the acidic hydrolysates of RB proteins were fractionated and the new ACE inhibitory triplet was isolated and identified. The amino acid sequence of the ACEI was identified as Ile-Thr-Leu or Leu-Thr-Ile. In vitro, ACE inhibition assays showed that the IC50 value of the peptide was 0.0118 mg.ml-1. The inhibitory activity of the peptide slightly increased after incubation with gast...
Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Muhammad Ibrahim Khaskheli2, Gul Bahar Khaskheli3, Allah Jurio Khaskheli2 and Arshad Ali Khaskheli1
...nding the area of Mithi (sandy desert zone), Tando Allahyar (irrigated zone) and Thatta (coastal mangroves zone) of Sindh province. The attributes like major nutrients (moisture, dry matter, total organic matter, inorganic/mineral matter (ash), ether extract, crude protein, crude fiber, nitrogen free extract and total carbohydrate contents) were included. Comprehensive survey indicated year round availability of 19 different vegetations at study areas whereby ...
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
... the application of spinosad that was also reported to cause resistance under extensive use. The aim of this work was to monitor the susceptibility to malathion after prolonged use in wild caught tunisian Ceratitis as well as to spinosad after a survey carried out with farmers and to give precise informations about treatment managem...
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1
... is a blood sucking abomasal nematode parasite of small ruminants producing the economic losses. The present study was conducted to explore the immunogenic properties of H. contortus crude proteins (HcCP). Protein profile of HcCP was checked by SDS PAGE and immunogenic proteins were recognized by the antisera produced by using the HcCP as antigen. Infective stage of the H. contortus (L3) was used for the challenge infection. Protein band pattern ...
Gulnaz Afzal1, Ghulam Mustafa2*, Shakila Mushtaq3 and Amer Jamil4
... javanus and Pardosa timidula were first ever spiders of whom any gene sequences were deposited in nucleotide database. A CO1 profile of all spider species was correctly assigned and 100% of subsequently analyzed specimens were identified up to their species levels. Such outcomes establish the potential of CO1 as a rapid identification tool and for phylogenetic relationships of spiders. Despite being sympatric species, the origin and d...
Muhammad Waseem, Tariq Mahmood*, Abid Hussain, Abdul Hamid, Faraz Akrim, Shaista Andleeb and Hira Fatima
...e bear from 10 different sampling sites. The sign density of black bear ranged between 17/km² (Paris MRF site) and 1.2/km² (Sharan site). Sign density was high in habitats having thick, dense and broad-leaved forest with steep slopes having the water resource/s. Sightings of the bear were the highest in the month of July (32%), and the least from November 2016 to March 2017. A total of 74 livestock were killed (in previous five years span) by Asiatic...
Yunfang Tian, Zeqin Fu, Jiantong Feng, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
... planktonic larval dispersal. The clam, Meretrix meretrix, is an important aquaculture species in the coastal regions of China. However, the previous studies of genetic information about M. meretrix were vague. None integrated research to reveal the population genetic and structure of M. meretrix in China from north to south. In this study, we investigated the genetic variation of seven location samples ...

Amit Sharma1* and Pankaj Sood2 

...ctors in order to obtain satisfactory results. It causes ultrastructural, biochemical and functional damage to the spermatozoa leading to reduced motility, viability, impaired transport and fertility. Caprine sperms are extremely sensitive to cryopreservation compared to other species which is mainly attributed to the compositional variation of the sperm plasma membrane. Different factors affect affects the semen cryopreservation viz. species, breed, semen co...
Seval Dernekbasi* and Emin Karatas
...t with canola oil (CO)], safflower oil trial (SFO; continuously fed by diet with safflower oil (SFO)], mixture oil trial (CSFO; continuously fed by diet containing both %50 CO and %50 SFO), alternate cycled feeding trial [(FO/CO; fish fed by diet with CO for 2 weeks and FO for the following 2 weeks), (FO/SFO; fish fed by diet with SFO for 2 weeks and FO for following 2 weeks) and (FO/CSFO; fish fed by diet with CSFO for 2 we...
Muhammad Yahya1, Muhammad Afzal1, Muhammad Zeeshan Majeed1*, Imtiaz Sarwar1, Khurram Shehzad2, Muhammad Luqman3 and 
Sher Muhammad Shahzad2
...), extensive random soil sampling was carried out from selected localities in district Sargodha (Punjab, Pakistan) and soil microarthropods (i.e. springtails and mites) were extracted from composite soil samples using Tullgren-Berlese funnel. Impact of collection seasons (i.e. spring, summer, autumn and winter) and impact of different land-use types or categories on the population abundance of soil microarthrop...
Asim Faraz*
...along with household refusals. Twice watering was provided. Fortnightly weighing of calves was done by digital scale. Data collected on different parameters was subjected to statistical analysis with 2×2 factorial arrangements of treatments under completely randomized design. After 120 days trial period the mean body weight and average daily gain (ADG) of male and female calves was significantly increased (P<0.05) in IMS as 80.8±2.7, 77.8&plusm...

Ihsanullah Kakr1, Sarwar Khan2, Khalid Khan3* and Sajjad Ahmed

...tan. The two districts Musa Khail and Jhal Magsi with thick populations of camels and have different climate and geographical distribution were selected. The questionnaire was developed for survey of Camel farmers and information regarding the age of respondent, experience, type of community, feeding/watering pattern of camels, prevailing camel diseases in the area, treatment facilities, traditional remedies used by them against various diseases in camels and ...

Muhammad Asif Farooq1*, Muhammad Jalal Arif1, Muhammad Dildar Gogi1, Bilal Atta2 and Ahmad Nawaz

...f a mango orchard. Fruit samples collected from all modules were subjected to analytical analysis using QuECHERS for sample preparation followed by quantification with GC-ECD. Recoveries of the samples analyzed were ranged from 78-98%. Results revealed that 75.00% samples of pesticide residues mitigation in PRMM-4 followed by PRMM-3 (66.66%), PRMM-2 (33....

Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq 

...und the world and at the same time it is perceived among the world’s 100 most essential intrusive species. Thiamethoxam and imidacloprid are Neonicotinoids with a minute toxicity and they have no teratogenic effects on grains, therefore both are used as cereals protectant against stored grain pests world widely. The recent study was planned to evaluate the efficacy of thiamethoxam and imidacloprid for the control of Trogoderma granarium under laboratory ...
Bo Gao1,2, Fengmei Yang3, Wei Chen2, Xiaojia Song4, Xiaobo Liu5 and Dongmin Li1*
...he log-rank test. At the same time, through receiver operating characteristic (ROC) curve analysis, their diagnostic threshold values were calculated. MDR1 expression levels were the highest in malignant tumor tissues, followed by adjacent tissues, and the lowest in non-cancerous tissues. Differences in expression level between varying degrees of differentiation and/or lymphatic metastasis, as well as variations in negative and positive expression between surv...
Savas Atasever*, Ali Vaiz Garipoglu and Huseyin Erdem
...ns (grazing (G), silage usage (S), compound feed usage (C), number of milking cow (NMC), calf suckling period (CSP)). Average F (3.144±1.931%), P (3.022±0.448%), L (4.475±0.669%) and logSCC (5.386±0.529) values were within acceptable ranges. The present investigation revealed that feeding practices had an important role on both milk quality and quantity of Jersey crossbred cows. Therefore, it is s...
Muhammad Rizwan Ashraf1, Asif Nadeem1,2, Eric Nelson Smith3, Maryam Javed1, Utpal Smart3, Tahir Yaqub4, Abu Saeed Hashmi1 and Panupong Thammachoti3
...elongs to genus Echis is saw scaled viper (Ehcis carinatus). Molecular techniques have made it easy to elucidate phylogenetic relationships and evolutionary histories of different groups of organisms. This study is the first attempt to find the phylogenetic relationship and diversity through mitochondrial genes in saw-scaled viper from Pakistan. Tail tip biopsies of Saw-scaled vipers were used for amplification of mit...
Peimin Yang*, Zongyun Hu, Yixin Liu, Guanghai Jin and Lei Wang
...ive populations, and the samples exhibited high haplotype diversity (h=0.8467) and low nucleotide diversity (Pi=0.0063). Genetic distances ranged from 0.0002 to 0.0149 within and 0.0007 to 0.0140 between populations. AMOVA analysis indicated significant genetic differentiation among populations (Fst=0.3202, P<0.01) and 68.98% of the total variation was resulted from intra-population differentiation. Pairwise...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
...p> and Xth passages was examined by immunohistochemical method using monoclonal antibodies. Determination the index of proliferationdogs adipose-derived stem cells (DADSCs) on IVth and Xth passages. Established that DADSCs contains multipotent stem cells, that are characterized by an almost homogenous fibroblast-like cells оn the IVth and Хth pas<...

Amjad Usman1*, Arshad Khan1, Ruidar Ali Shah2 and Toheed Iqbal3 

...ustify;">Scirtothrips dorsalis is a devastating pest of tomato. The objective of this study was to screen commercially available tomato genotype and to identify the morphological plant characters responsible for resistance against S. dorsalis. For this purpose, five commercially cultivated tomato genotypes (Riogrande, Riogrande H, Bombino, Roma VF and Roma) were evaluated against thrips (S. dorsa

Arjumand Nizami1, Muhammad Zulfiqar2*, Jawad Ali1, Naushad Khan2 and Imran Sheikh

...n December 2018 from the same beneficiary farmers. The data collected were analyzed using MS-Excel. The analysis of the data reflected that although canal water was available but about 80% at head, 50% at middle and 20% at tail. Thus remaining irrigation water requirement i.e. about 20% at head, 50% at middle and 80% at tail was met through tube well operation. The result from before and after AWD technology and laser land leveling technology adaption shows th...

Bahadar Sher Khattak1, Muhammad Kaleem1, Waqas2*, Humaira Naz1 and Muhammad Yasir

... Tetara, of District Charsadda. These two UCs were purposively selected to collect 150 samples for the study. A well-structured interview schedule was developed to collect data which was pre-tested before starting the actual process of data collection. The total sample size of the study area was 150, randomly selected for interviews. After the data analysis, it was found that the majority ...
Jing Zhang1,2, Xin Wang2, Yun Zhao2, Yongcheng Jin2, Yongfeng Zhou2, Junmei Wang2, Yurong Fu2, Rui Wang2, Ruihua Li2, Hengtong Fang2 and Hao Yu2,*
...inal dysfunction and mucosal immune disorders, the effect of zearalenone (ZEA) on intestinal mucosal function remains controversial. We aimed to explore the effects of short-term ZEA exposure on the mucosal barrier function in the colon. We found that the morphology of colonic mucosa in mice was normal after ZEA was administered by gavage for one week, t...
Gong Ga1,2 and SuoLang Sizhu2,*
...In this study, 173 serum samples of Tibetan swine were tested for anti-HEV IgM and IgG antibodies by the ELISA. HEV RNA were measured in feces (n=173), serum (n=173) and tissue ((including liver, spleen, kidney and intestine, n=21) by nested RT-PCR and qRT-PCR. HEV antigens were detected in tissues by immunohistochemistry analysis. Overall, we found that that eight serum
Xuhui Zhang1, Zhiyuan Sun2, Jinfeng Cai1, Guibin Wang1, Zunling Zhu1, Linguo Zhao3 and Fuliang Cao1,*
...ol group, fed with the basal diets; NF group, the addition of 3 g/kg NF products; FR1, FR2 and FR3 groups, addition of FR products 1 g/kg, 3 g/kg and 6 g/kg, respectively. There was no difference in body weight gain, feed intake and feed intake/gain ratio between treatments. Compared with the control group, the percentage of the abdominal fat, sarcous fat and lactic acid (LD) for FR groups were significantly decreased. Birds...
Aftab Shaukat1,*, Sana Hanif2, Irfan Shaukat3, Muhammad Ahsan Naeem4,5, Shadab Shaukat6, Rizwan Shukat8, Shahid Ali Rajput9, Imran Shaukat10, Mubashar Hassan4, Khalid Mehmood7, Saqib Umar11 and Muhammad Ali Jamil1
...-0.1px;">miRNAs (hsa-miR-939-3p, hsa-miR-661, hsa-miR-2861, hsa-miR-23b-5p, hsa-miR-23a-5p, hsa-miR-4764-5p, hsa-miR-6734-3p, hsa-miR-4441, hsa-miR-4267, h
Celalettin Gözüaçik1,*, Mustafa Güllü2, Ayda Konuksal3, Reşat Değirmenci3 and Ercan Akerzurumlu3
...Güzelyurt, Gazimağusa and İskele townships in 2012-2013 years, and 1567 and 862 larvae were collected in the two years, respectively. Larvae were kept inside boxes covered with nets in the laboratory at 25±1◦C and 65±5% r.h. Following pupation, adult moths and parasitoids were obtained and percentage of parasitized larvae was determined. At the end of the study, Bracon (Habrobracon) stabilis (Wesmael) (Braconidae) an...

Aftab Khan1, Shahid Ali1*, Asim Khan1, Muhammad Waqas1 and Sufyan Ullah Khan2 

...ected through multistage sampling. Parametric technique of stochastic frontier production function was estimated to model output of maize growers. Results of stochastic frontier model revealed that tractor hours, labor and FYM positively and significantly affected yield of maize. Average technical efficiency was predicted to be 0.68 ranging from 0.31 to 0.91; implies that an average maize farm can increase yield by 33 percent with existing technology. Technica...

Rehman Ullah Khan* and Muhammad Ayub 

...a aloe vera blended pulp samples of all treatments were packed in pet bottles and stored at ambient conditions for six months. The results revealed significant decline in pH from 4.51 to 3.47, ascorbic acid from 120.42 to 51.48 mg/100gm, total phenolic content from 32.43 to 11.47mg gallic acid equivalents/100g and antioxidant capacity from 76.02 to 33.23TE/100g while significant increase was observed in total soluble solids from 5.05 to 6.210Brix, total acidit...
Naseeb Ullah1, Assad Ur Rehman1, Aamir Khan1, Rabia Noushin2Shahid Naiz Khan1, Syed Ishtiaq Anjum1, Sajida Afzal1, Asma Naseem1Kenza Javed1, Ujala Rauf1 and Kalim Ullah1*
...(KP) Pakistan. 376 blood samples were randomly collected from livestock (large and small ruminants) from December 2017 to April 2018 to examine the prevalence of FMD. The current study indicated that overall FMD in Livestock was 14.31% with 16.37% in small ruminants and 12% in large ruminants. A significant difference in sero-positivity was observed in small, young and adult of both small and large ruminants. The sero-positivity was highest in adult animals (2...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...ups C1 and C2) for immunisation. Blood samples were collected at 0, 3, 6, 10 and 14 weeks after primary immunisation for the detection of kisspeptin antibodies and testosterone by indirect enzyme-linked immunosorbent assay. Scrotal circumference was detected from week 0 to week 14 after primary immunisation, and the te...

Muhammad Farhan1, Ghulam Murtaza1, Muhammad Ramzan2, Muhammad Waqar Sabir3, Muhammad Arsalan Rafique2, Saif Ullah

...rsity of Agriculture, Faisalabad. Chrysoperla carnea was found very active and consumed all stages of aphid. The predation rate of Chrysoperla carnea was increased with increase in larval instars (1st to 3rd). Third instar of Chrysoperla carnea was very voracious feeder and fed large number of aphid instars nymphs (1st, 2nd and 3rd). The larval predatory potential was 413.9±0.07 aphid per larvae. The current study results revealed that Chrysoperla carne...

Pushpa1, Nighat Seema Soomro1, Shahla Karim Baloch2, Mehmooda Buriro1, Aijaz Ahmed Soomro1, Muhammad Tahir Khan3, Qamar Uddin Jogi1*, Muhammad Nawaz Kandhro1 and Farheen Deeba Soomro1 

...(496.9) was also seen in same variety under the maximum treatment of Avena fatua. In respect of the above findings it was concluded that the powder from weed species reduced germination and subsequent plant growth of wheat which hints towards importance of apposite measures within due time against weed species to harvest better crop yield. 

...

Muhammad1*, Muhammad Suleman Bacha2 and Syed Akhtar Ali Shah1 

...re concerned about the unsafe disposal of solid waste and are willing to pay for organic fertilizer alternative to chemical fertilizer for urban agriculture. We also found that socio-economic variables namely education, monthly expenses, and agriculture farm size positively affect farmers’ WTP for organic fertilizer. Conversely, households’ size and land tenure of the farmer has a negative influence on farmers&rs...

Saeed Ahmed Shah Chishti1, Saba Aleem1*, Iram Sharif2, Kashif Nadeem1, Nusrat Parveen1 and Muhammad Najeebullah1 

...rminate tomato varieties saandal and sahel were tested in two minimum night temperature conditions 12.5-23°C and 2-7.5°C. Tomato yield per plant, yield per cluster, number of fruits per cluster, fruit weight and fruit diameter were significantly improved by the application of 4-chlorophenoxy acetic acid. Highest yield per plant 13.50 kg was recorded with the application of 75 ppm in 4- chlorophenoxy acetic acid twice...

Humayun Saleem1, Faisal Sohail Fateh2*, Zia-Ur-Rehman1 and Muhammad Abu Bakar Siddique

...mated from market random samples, and later the index was measured. The results showed that in all markets with varying degrees of disease incidence, frequency and disease index the prevalence of shot hole disease was 100 per cent. It was concluded that the markets near poor colonies are merely selling cheap fruits and they are not concerned with the diseased fruits. 

...

Noor-us-Sabah1*, Mukkram Ali Tahir1, Ghulam Sarwar1 and Sher Muhammad

...t of fertility status of salt affected soil was studied in a pot experiment conducted at research area of University of Sargodha in year 2017-18. A saline sodic-soil with desirable characteristics (SP = 40.35, ECe = 5.17 dS m-1, pHs = 8.71, SAR = 18.80 (mmol L-1)1/2, bulk density = 1.49 g cm-3) was selected for present study. After chemical analysis, dif...

Adewumi Olubusuyi Moses1,2*, Faleye Temitope Oluwasegun Cephas,1,3, Ope-Ewe Oludayo Oluwaseyi1, Ibitoye Ibipeju Henrietta1, Arowosaye Abiola Opeyemi1, Adelowo Oluwatosin Deji1 and Adeniji Johnson Adekunle1,2,4 

...ustify;">Using 40 faecal samples from acute flaccid paralysis (AFP) cases collected in 2019, we examined how coupling Enterovirus (EV) isolation in RD and MCF-7 cell culture with direct detection from clinical sample might impact our ability to detect non-polio EV Species C (NPESC) members in stool samples. In all, 20% (8/40) of all the samples screened ...
Qaisra Siddique1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3

 

...tress for two months and samples were collected on weekly basis. It was noted that GST level varied significantly with duration. The GST level was raised in first 28 days after that it was dropped off up to 56-day. The trend of GST in fish tissues was observed as muscle<heart<brain<kidney<gills< liver. However, TPCs in selected tissues of stressed fishwas lower significantly as compared to control. 
Waqas Ahmad Shams1*, Gauhar Rehman1, Khurshaid Khan1, Ibrar Ahmad1, Saima Bibi1, Saif Ul Islam2, Dawood Safi1, Saba Gul Ayaz1
..., collected at different sampling points. Extensive surveys were conducted for three years at different timings during the active periods of crab life. To have a comprehensive study of the crab fauna of Buner District, nine different localities were selected for conduction of survey. The results showed that there were two crab species yawi and Smalley. These species belong to Allacanthos. They have gonopod with a distal portion of mesio laterally flattened. Th...
Mubashar Hussain1*, Misbah Younas1,Muhammad Faheem Malik1, Muhammad Umar1, Maimoona Kanwal1, Moazama Batool2
...ges on local scales. The sampling was accomplished by surveying grassy fields, croplands, old dung piles and fresh dung pats from selected localesofSialkot during 2016. Specimens were collected by hand picking and cattle dung baited pitfall traps. Sixteen species representing three guilds i.e. Paracoprid (10 species), Endocoprid (4 species) and Telecoprid (02 species) were recorded. Onitis excavatus (27.68 %)and Onitis crassus (9.59 %)showed maxi...

Ahmad Naeem Shahzad1, Muhammad Kamran Qureshi2, Samee Ullah1, Muhammad Latif3, Shakeel Ahmad1 and Syed Asad Hussain Bukhari1* 

...e detrimental effects of salt stress, in cotton plants. Three concentrations of trehalose (0, 5 and 50 mM) were applied to plant foliage, subjected to varying levels (0, 11 and 17 dSm-1) of salinity. Salt stress disturbed the sodium and potassium concentrations in leaves and roots of plants. Different morphological traits of plants including number of leaves, leaf fresh weight, shoot fresh weight, root fresh weight, plant he...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...ary to July, 2017. Blood samples were collected from all age groups patients visiting hospital labs and District Head Quarter Laboratory who were recommended for diagnosis of malaria. About 1050 patients were examined and malaria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found to be infected with malaria parasite. Data was also collected sfrom other labs of District Swat which accumulate...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
... disease has trapped thousands of people in Karachi region of Pakistan and the symptomatology among suspected cases was compatible with Chikungunya fever. Thus Chikungunya outbreak was reported in Pakistan but actually the recent reports about the occurrence of Chikungunya outbreak in the southern region of Pakistan has been highly controversial. In order to further confirm the circulation of the virus and put an end to the speculative claims for the correspon...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
...valuate the effect of Ca salts of palm fatty acids on milk production, milk fat, plasma metabolites, and reproductive parameters in early-lactating Nili Ravi buffaloes. Sixteen early-lactating Nili Ravi buffaloes received 2 treatments in a completely randomized design with a period length of 120-d. The treatments were 1) Ctrl = basal diet without supplementation of fat, 2) Ca-FA = basal di...
Wen Qin1,2, YanGan Huang1, Lei Wang1, Gonghua Lin1, Jundong Yang1,2, Pengfei Song1,2, Hongmei Gao1,2, Jingjie Zhang1,2 and Tongzuo Zhang1,3,*
...le (Gazella subgutturosa) mainly live in arid and semi-arid environments. In Qinghai Province, goitered gazelle are found only in the Qaidam Basin, which shows pronounced seasonal changes ranging from cold, dry winters to short, hot summers. The gut microbiota plays a very important role in host health, immunity, and digestion. Therefore, seasonal changes in gut microbiota can affect host digestion and metabolism in ways that help the host adapt to diff...

Grigory Antonovich Nozdrin1*, Regina Gabdrahmanovna Utkina2, Anastasia Aleksandrovna Lelyak2 and Yana Viktorovna Novik1 

... preparation once with dosages (2,000 µl/kg, 3,000 µl/kg, 4,000 µl/kg, and 5,000 ml/kg) while the fowl in the reference group were left untreated (they were given a placebo – distilled water). The experimental fowl were monitored every hour during the first day, and every day afterward for a total of 14 days. The live body weight was measured every week; the overall condition of the fowl, behavior, physical activity, water, and feed con...
Sikander Ali* and S. Wajiha Khalid
...m i.e., sucrose minerals salt at pH 5.5 was used. Different physical parameters were applied for maximum enzyme production. The enzyme exhibited maximum activity at pH 6 after 72 h of fermentation (158±0.39 U/ml). The partial purification of the enzyme was done with ammonium sulphate (20-80 %) followed by dialysis which resulted in 21 % purification from the crude enzyme extract. The molecular weight of enzyme was found to be 86 kDa, as determined by SD...

Jie Yang

...al-luciferase reporter assay, qRT-PCR and Western blot, the mRNA and protein expression were detected, respectively. Cellular apoptosis were examined by flow cytometry and Western blots was applied to assess the cleaved caspase-3, -8, -9, and cleaved poly-ADP-ribose polymerase. Protein expression of extracellular-regulated kinase were detected. Finally, data analysed statistically to determine significantly differences among groups. Results showed that breakpo...

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

...is. A total of 1608 milk samples (793 from cattle and 815 from buffaloes) were collected from already PPD (purified protein derivatives) tested lactating animals aseptically. The data obtained were finally analyzed statistically using chi squared test. The Mycobacterium was identified through ZN staining, culture and PCR. Out of 1608 milk samples, 60 (3.73%) were found positive for acid fast bacteria through ZN staining wher...

Amjad Ali1*, Lal Almas2, Syed Attaullah Shah1, Hina Fatima3 and Asim Khan1  

.... Khan, Malakand and Charsadda) of Khyber Pakhtunkhwa (KP). Through a structured questionnaire, key questions were asked to gather data with respect to inputs utilization and socio-economic characteristics of selected sample of sugarcane growers. Using Stochastic Frontier Approach (SFA), mean technical efficiency of sugarcane crop in KP was around 70 percent ranging from minimum 53 to maximum possible efficient level of 98 p...
Qingzhan Ma1, Bo Wu1, Jianping Jiang2 and Zhaobin Song1,3*
...ial breeding. Eight microsatellite markers were used to analyze genetic characteristics of eight channel catfish populations (POPA, POPB, POPC, POPD, POPE, POPF, POPG, and POPH). The average polymorphic information content (PIC) ranged from 0.525 (POPD) to 0.733 (POPA) indicating high polymorphism (PIC > 0.5). The average observed heterozygosity (Ho) and expected heterozygosity (He) in the eight populations ranged from 0.504 (POPH) to 0.767 (P...
Quratulain1, Ata-ul-Mohsin1, Muhammad Naeem1, Ghulam Shabir1, Muhammad Khalid Rafique2 and Rashid Mahmood2*
...b red, Swat-1, Hazara, Nasarpuri, Red imposta, VRIO-7, VRIO-8 were evaluated during rabi season of 2017-18 at Chak Shahzad, Islamabad (33o 40 N and 73o 08 E), for their susceptibility to onion thripsunder field conditions. Seeds of different onion varieties were obtained from Ayub Agriculture Research Institute Faisalabad (AARI), National Agriculture Research Center Islamabad (NARC) and BARC Quet...
Saeed Fatima1, Khalid Javed Iqbal1, Usman Atique2,3*, Arshad Javid4, Noor Khan2, Sonia Iqbal2, Hamid Majeed5, Hamda Azmat2, Bakhat Yawar Ali Khan6, Irfan7, Muhammad Tausif Shahid1, Gulnaz Afzal1
...les, sediments and water sampled from Head Punjnad (HP), Pakistan during 2017. The likely reason for the selection of HP as a study target was its emergent prominence as an irrigation hub and holidaymakers spot largely for angling and feasting. The investigated five fish species are Ctenopharyngodon idella, Oreochromis niloticus, Eutropiichthys vacha, Rita rita and Sperata sarwari. The resu...
Muhammad Shuaib Khan1, Shakeeb Ullah1, Nisha. A.R2, Muhammad Umar3, Muhammad Inamullah Malik1*, Syed Muhammad Kamal Shah1, A. Zaman1, Shakir Ullah1, Imdad Ullah1, Sumera Ali Khan4
...ood for domestic use and sale in the nearby local market to cope their daily needs. The present study was carried out in three different rural towns of Dera Ismail khan district of KP in the year 2016, where the house hold community members male and female keeping animals to cope their livelihood needs. By restoring proportionate sampling each group was comprised of 30 males 30 females. Animal’s owners were selected fo...
Salahud Din1,*, Saima Masood1, Hafsa Zaneb1, Habib ur Rehman2, Saima Ashraf1, Imad Khan3, Muqader Shah4 and Syed Abdul Hadi1
...id, frontal, parietal, nasal, incisive, turbinates, mandible and hyoid apparatus. The bony region of the cranium of Chinkara was comprised of occipital, ethmoid, sphenoid, interparietal, parietal, temporal and frontal bone. The foramina identified in the facial region of the skull of Chinkara were, infraorbital, supraorbital foramen, lacrimal, sphenopalatine, maxillary and caudal palatine foramina. The foramina of the cranium of the Chinkara skull were the int...

Ahmad-Ur-Rahman Saljoqi1, Muhammad Zubair Khan1, Ayesha Bibi2, Muhammad Shehzad Khan1*, Bashir Ahmad

...hwaraskay (Dodonaea viscosa). Morphological traits such as plant height, maximum number of fruits plant-1 and yield were observed and severity of disease was investigated for supplement efficacy. The biochemical results obtained were all positive, which confirmed stem rot pathogen as Erwinia carotovora sub spp. chrysanthemi (gram-negative bacteria). Highest plant height (75.3 cm) and maximum number of fruits plant-1 (14.3) w...

Rabia Iqbal, Muhammad Naeem*, Samrah Masud and Abir Ishtiaq 

...eir wet body weight. Ten samples from each hapa were randomly selected at the end of three months (90 days trial) for body composition analysis. Mean percent water, ash, fat and protein contents were ranged 80.55±0.94 to 80.55±0.94; 2.23±0.13 to 2.23±0.13, 5.85±0.55 to 6.63±0.97 and 10.59±1.67 to 11.93±1.67 in wet body weight of hybrid fish (Labeo rohita ♀ and Catla catla ♂), respectively, reared unde...

Muhammad Safdar Hussain1*, Muhammad Farrakh Nawaz2, Muhammad Ayyoub Tanvir2 and Noor-E-Hira

... 50 % drought to achieve said objective. It was found that drought negatively affected plant growth. The mean heights of E. camaldulensis and T. aphylla were maximum (38.16 and 34.7 cm, respectively) at control irrigation while minimum height was found (17.1 and 24.9 cm, respectively) at 50% drought. So, the reduction in plant height of E. camaldulensis was more (55.6%) as compared to T. aphylla (28.1%). The reduction in root length of both species was 19% and...

Mubashir Mehdi1*, Muhammad Bilal Ahsan1, Burhan Ahmad1, Khuram Nawaz Sadozai2, Gulnaaz Hameed3 and Muhammad Asif4 

...in system under the food safety and quality standards, they have more potential and prospective to improve the livelihood of the on-farm workers. Education included as schooling years was found major regressor for the income of on-farm laborers. In ceteris paribus conditions, coefficient of education indicates that there is a positive relationship among education of labor and Income. Credit facility at farm level was also incorporated as dummy variable under e...

Cornelius Arome Omatola*, Samson Daniel Iyeh, Shedrach James Abuh, Charles Kehinde Mofolorunsho, Martin-Luther Oseni Okolo and Phoebe Queen Akoh 

...c women in Anyigba. Sera samples from a cross-section of 250 consenting pregnant women on antenatal visits to five health facilities were screened for HIV, HBV, HCV and HSV-2 using commercially available immunochromatographic based kits and for syphilis using non-treponemal and treponemal antibody test. Socio-demographic and obstetric parameters were obtained using structured questionnaires. Sixty-four (25.6%) out of 250 women had at least one STI. The overall...

Aqsa Ahmad1, Iftikhar Ahmad1* and Malik Fiaz Hussain Ferdosi

... with variable ratios of saw dust (SD), peanut hulls (PH) and sugarcane pressmud (a by-product of sugar industry, which is rich in nutrients) or coco-coir (CC), rice hulls (RH) and sugarcane pressmud (PM) were used. Seeds were sown in plastic plug trays containing CC, silt (soil extracted from canal) and PM (1:1:1; v/v/v) substrate for seedling development and transplanted at the two to four leaf stage into lily crates with the treatment substrates. Plants gro...

Gulnaz Hameed1, Abdul Saboor1, Khuram Nawaz Sadozai2*, Ghaffar Ali2, Dawood Jan2 and Mansoor Rasheed

... in AJ and K. Multistage sampling technique was used to draw a sample of 540 respondents from the universe comprising both registered and nonregistered members of AJKCDP. Primary data were collected through interview schedule. Foster Greer and Thorbecke measure was used to calculate incidence of poverty which is then used to model the probability expressions with the help of Logit to investigate the correlates of poverty in ...

Muhammad Usman Saleem, Nadeem Iqbal, Shawaiz Iqbal*, Usama Bin Khalid, Adila Iram, Muhammad Akhter, Tahir Latif and Tahir Hussain Awan 

...n: justify;">Rice (Oryza sativa L.) is a main staple food in Pakistan, after wheat and is generally cultivated through raising nursery seedlings and transplanting them in puddled soils. Declining surface water, high cost of pumped groundwater, high labor costs, and drudgery in manually transplanted rice (TPR) have motivated researchers to develop alternated technology such as direct seeded rice (DSR). About 30% of total water used in rice cultivation is consum...
Ali Uzun* and Bilgenur Yasa
...roup formation and dispersal on the lake surface was studied. Coots form large groups in winter (as many as 11,500 individuals). The total number of individuals has no effect on dispersal on the lake surface and grouping behavior of the species (p>0.05). Temperature and security are the primary effective factors for these subjects (p<0.01). Behaviors related to security developed simultaneously and became normal in a s...

Khalid Khan* and Guo Xiangyu 

... to provide all the necessary training and consultation to the farmers to improve the technical efficiency of the rice producers in the district. 

...

Ghulam Akbar1*, Muhammad Anjum Zia1, Ali Ahmad2, Neha Arooj1 and Shahneela Nusrat

...use. However, it is necessary to truncate antigenic regions present in the SK gene for the removal of streptokinase antigenicity. The basic goal of this research work is to highlight antigenic regions founded in streptokinase molecule and to give strategy to remove these antigenic sites. 

...
Şükrü Önalan1*, Muhammed Arabacı1and Haşmet Çağırgan2
... with 0–6.5 % NaCl salinity. As a result of the examination of biochemical properties with API Rapid ID 32 Strep test, it was observed that 2 L. garvieae isolates were different from other isolates in respect of sucrose utilization. 1 and 19 number isolates were negative for sucrose whereas other isolates gave positive results. 1 number isolate was different from other isolates based on maltose profile. While isolate 22 was maltose negative, the o...

Faisal Hafeez, Asad Aslam*, Ayesha Iftikhar, Afifa Naeem, Muhammad Faheem Akhtar and Muhammad Jawad Saleem 

...y insecticides, that are safe and harmless for nature and environment. 

...

Fazal Haq*, Asia Parveen, Safdar Hussain and Arshad Hussain 

...ed population and random sampling technique is used to select the sample. Self made questionnaires, filled through interviewed process from the random selected farmers were used as a tool for collecting primary data. The raw data is tabulated and analyzed using Microsoft Excel. The analyzed data is used to develop the mathematical model. Simplex method is applied to find the optimal solution of the model, which showed 10.18%...
Aamir Mushtaq1,2, Rukhsana Anwar1 and Mobasher Ahmad1,2,* 
...’s disease a universal challenge, this study was planned to utilize the bio-constituents of Pimpinella anisum dried seeds to check the oxidative neuronal decay in brain parts. Methanolic extract of Pimpinella anisum (meP.a) dried seeds was explored qualitatively and quantitatively for analysis of phytoconstituents along with anti-oxidant activity. Anti-amnesic activity was found by behavioral and biochemical studies for which male albino mi...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin acetate (Buserelin® aniMedica, Poland). After equilibration, the straws were frozen in liquid nitrogen vapour using a computer controlled automatic freezer. After one day, straws were...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
... total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 from cattle. Among the the 4 ORFs,...
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
...ferent solvent extracted samples of Ziziphus mauritiana var. spontanea and Oenothera biennis against different microbes. Ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea measured the highest zone of inhibition against Staphylococcus epidermidis, Escherichia coli and Salmonella typhi. The MIC values of ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea were the ...
Muhammad Asim1, Zaheer Ahmad2, M. Akbar Khan1, M. Adeeb Babar1, Sayyed Ghyour Abbas1,*, Muhammad Khalil Nawaz1, Kiran Aftab3 and Muhammad Akhtar4
...i>Tragoportax cf. salmontanus have been recovered from the Chinji Formation of the Lower Siwalik Subgroup, Pakistan. The remains comprise horn-cores, maxillary and mandibular fragments, and isolated teeth. Having compared with the previously described bovid remains from the Siwaliks, the new material has been allocated to Tragoportax cf. salmontanus. This species is well represented in the Middle Siw...
Imran Khan1, Muhammad Nawaz1,*, Aftab Ahmad Anjum1, Mansur-ud-Din Ahmad2, Adnan Mehmood1, Masood Rabbani1, Amina Mustafa1 and Muhammad Asad Ali1
...um IKP 111 and L. salivarius IKP 333 on histomorphology of small intestine and D-xylose absorption capacity in broiler challenged with S. enteritidis. Negative control group was not supplemented with probiotics. Positive control groups received only the challenge bacteria (S. enteritidis) ATCC 13076 at day 07. Groups (3, 4 and 5) received probiotics (IKP23, IKP111, IKP333) at day 01 to 35 and challenge bacteria at day 07 in prevention ...

Asmaa Abdallah Darwish 

... disorders group). Blood samples were collected from all ewes and vitamins concentrations were estimated in all groups. The results of this work demonstrated a significant (P< 0.05) decrease in vitamin E, A, D3 and C concentrations in inflammatory diseases groups, late pregnant groups and postpartum groups. Conclusion: antioxidant hypovitaminosis partially participate in different ovine diseases pathogenesis and complications and their supplement for health...
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...ve sequencing of 18 cDNA samples derived from male adults, female adults, and pupa of A. cinerarius in southern and northern Xinjiang. We extracted total RNA from 18 samples and synthesized cDNA via reverse transcription. Then, Illumina sequencing was performed and unigenes were identified after assembly and clustering. The resulting unigenes were then compared to homologous sequences using NCBI-NR, Swiss-Prot, KOG, P...
Sana Khan1, Raheela Taj1, Noor Rehman2, Asad Ullah3, Imad Khan3 and Sadeeq ur Rahman3,*
...A total of 2950 clinical samples were collected from both hospitalized and non-hospitalized patients of Khyber Teaching Hospital (KTH), Peshawar, and processed by for isolation of C. freundii followed by phenotypic detection of β-lactamases and antimicrobial susceptibility profile. Our results indicated that a total of 130(4.40%;130/2950) samples were positive for C. freundii comprised of urine 58.4...
Shaista Abbas1,Imtiaz Rabbani1, Hafsa Zaneb2, M. Shahbaz Yousaf1, Saima Ashraf2, Abid Hussain Shahzad3, M. Afzal Rashid4 and Habib Rehman1,*
...d YSC10 and were fed a basal diet supplemented with 0g, 5g or 10g yeast day/animal, respectively for 4 weeks before and -4 weeks after kidding. When compared with the non-supplemented goats, dietary yeast improved (P<0.05) DMI during transition period however, BCS (P = 0.81) and BW (P = 0.59) did not vary amongst the groups. During postpartum period, results of the serum biomarkers revealed higher glucose (P=0.02) and lower (P = 0.002) non-esterified fatty ...
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...ota (against NDV) and Massachusetts (against IBV) vaccine preparations. After screening the protective titers in birds at 19 days post inoculation three week-old broilers were challenged with 10-5.76 and 10-6.03 EID50 dose of NDV and IBV, respectively. The hosts were divided into six groups of 35 birds each. These were groups A (NDV-challenged), B (IBV- challenged), C (NDV+IBV-challenged), D (IBV+NDV challenged), E (NDV and IBV...
Fermín López-Uriostegui1, Jesus T. Ponce-Palafox2*, Fabiola Lango-Reynoso1, María R. Castañeda-Chávez1, Itzel Galaviz-Villa1, Sergio Castillo-Vargasmachuca2 and Arturo Ruiz Luna3
..., 29, and 32ºC) and salinity (0, 5, and 10‰) on growth performance and survival of Macrobrachium americanum was studied under controlled laboratory conditions. Sub-adult’s prawns (10.29 ± 0.27 g) were reared in these conditions for 72 days. Each experimental group contained 25 prawns in an approximately 100-L water capacity tank. The experimental design was factorial with three repetitions per treatment. Prawns were fed (commerc...
Mahvish Rajput1, Abdullah G. Arijo1, Muhammad Bachal Bhutto1Rehana Shahnawaz Buriro2, Javaid Ali Gadahi1, Muhammad Naeem3 and Zubair Ahmed Laghari1*
... prevalent in all months sampled but the highest infection rate was observed in November (56%) followed by October (38%), September (34%) and was observed lowest in August (22%). While, the infection rate in both sexes varies but the statistically non-significant difference in the prevalence among females (45%) than males (29%) was observed. Among the isolated rumen flukes, six flukes were used for molecular characterization by amplifying second internal trans...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...iniae as the causative agent of the disease outbreak. The bacteria has a unique biochemical profile compared with most of the previously isolated strains. Antimicrobial susceptibility tests revealed that S. iniae HNM-1 is resistant to most of the important antibiotics, including Kanamycin, Amoxicillin, gentamycin, Penicillin G and spectinomycin. The bacteria is pathogenic to Chinese sturgeon via intraperitoneal injection. It instigated patholog...
Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3
...ci L. (Thripidae: Thysanoptera), release of Coccinella septempunctata and Chrysoperla carnea, spray of Neem Seed Kernel Extract (NSKE) 5% and spinosad 240 SL (Tracer) were integrated in all possible combinations, thus, making a total of 15 treatments including separate application of each above and a control where none was applied except water only. Results showed significant difference amongst treatments (...

Anila Latif, Zaheer Abbas, Farhatullah and Ghulam Muhammad Ali* 

...se PCR. Whole plant bioassay confirmed 100% mortality in DBM within 72 hours. The present investigation concludes that the metabolic engineering of berberine in plants is an effective strategy to protect plants from herbivorous insects and this pathway should further be evaluated for possible application in agriculture. 

...

Muhammad Usman Ghazanfar1, Waqas Raza1*, Waqas Wakil2,3, Imtiaz Hussain4 and Misbah Iqbal Qamar

... L.) with application of salicylic acid (SA) and β-aminobutyric acid (BABA). Concentration of SA and BABA (2, 4 and 5 mM) were applied to foliage. Application of both resistance inducers suppressed disease development by 54.1% and 67.4%, for SA

Hasnain Alam1*, Jabar Zaman Khan Khattak1 and Taoufik Saleh Ksiksi

... best under both induced salt and drought stress at the germination stages. It is concluded that all four-selected species successfully germinated under maximum osmotic potential understudy which is not suitable for landscape species. All four investigated species can be further evaluated for field performance under stress conditions so can be recommended for mass vegetation and as a substitute to exotic landscaping species. 

...

Ahmad Kiani1*, Shapour Zarifian1, Hosein Kouhestani1 and Hosein Karimzadeh

...r weight, So, it is necessary to develop transportation to streamline access to market and also the industries need investors to be flourishing; Accordingly, both governmental and non-governmental organizations could take part to improve industrial sector. Moreover, the output of research shows some geographical factors like climate and geology have the least importance as to development and establishment of industries in the rural areas, and they are not para...

H. Liu1, X.P. Tang1, R.J. Fang1,*, F.Yi2, C. Zhang2, R.Q. Yang1, F. Sun1 and S.Y. Zhou1 

...ontrol group was fed a basal diet, experimental groups were fed diets supplemented with copper amino acids addition level was 25, 50, 100 and 150 mg/kg Cu or 150 mg/kg Cu from CuSO4 of the control group,respectively. Growth performance, serum biochemical parameters (Cu-Zn SOD activity) and diarrhea incidence were measured in the 2-phase feeding program (1-14 d, 15-42 d). Results showed that: compared with the control diets, 1) Diets supplemented wit...

Tingting Li, Shuangshuang Geng, Long Xie, Huiyan Xu, Aolin Luo, Pengwei Zhao, Huan Yang, XingWei Liang, YangQing Lu, XiaoGan Yang* and KeHuan Lu*

...e-linked immunosorbent assay (ELISA) results showed that GDNF gene secretion was also significantly increased in PB-GDNF-Sertoli cells compared with that in control Sertoli cells (p<0.05). We further compared two types of spermatogonia cultured on either PB-GDNF-Sertoli cells or control Sertoli cells, revealing that the use of PB-GDNF-Sertoli cells as a feeder layer could significantly ...
Rabia Saeed1, Muhammad Naveed1, Muhammad Razaq2*, Muhammad Rafiq1, Muhammad Tahir Jan1, Syed Ishfaq Ali Shah1, Shabana Wazir1 and Fahad Munir3
...n growing areas of Dera Isamil Khan, Muzafar Garh and Multan districts, Pakistan.
...
Shaheen Rahman1, Kafeel Ahmad1*, Niaz Ali2, Muhammad Idrees3 and Waqar Ali4
...om local soil. Five soil samples were collected from research farm of Agricultural University Peshawar, Pakistan. Soil samples were processed for the isolation of A. tumefaciens. Various biochemical and morphological tests were conducted to confirm the identity of A. tumefaciens. The pathogenicity of the isolates was confirmed by in vitro carrot and potato discs inoculation assa<...
Tehreem Usman1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3 and Qaisra Siddique1
...PC decreased with the passage of time. Among both treatments, ES+CPF caused greater decline in CAT activity and TPC. It was concluded that insecticides in mixture form are toxic to fish and response of biomarkers depends on the type of toxicants and exposure duration.
...
Muhammad Tahir Waseem1, Abdul Majid Khan1*, Saliha Khalid1, Rana Manzoor Ahmad1,2, Ayesha Iqbal1, Muhammad Ameen1
... hypsodonty. The present sample add valuable information on systematics, dental morphology and dietary habits of the Late Miocene (7-5 Ma) boselaphines which are represented today by their living kin Boselaphus tragocamelus and Tetracerus quadricornis
Dil Afroza Khanom1, Amirun Nesa1, Muhammad Abu Sayed Jewel1, Muhammad Ayenuddin Haque1, Alok Kumar Paul1, Sonia Iqbal2, Usman Atique2,3*, Lubna Alam4
...n after wet digestion of samples. The results displayed the hierarchy of average levels of the targeted metals in G. chapra in declining order of Zn (14.37) > Cu (12.63) >Mn (1.39) > Cr (0.64) >Pb (0.38) > Cd (0.03 mg/kg) whereas, Zn (11.89) > Cu (11.18) >Mn (1.69) > Cr (0.52) >Pb (0.19) > Cd (0.01 mg/kg) in E. vacha. The t-test indicated significant differences (P<0.05) between Pb and...
Afzal Nimra1, Zulfiqar Ali1*, Safdar Sidra 2, Rida Ahmad1
...w enforcement against causative factors. 
...

Abid Khan*, Mukhtar Alam and Yousaf Jamal 

...sts (Saccharomyces cerevisae, Candida utilis), actinomycetes (Streptomyces albus, Streptomyces griesus) and fermenting fungi (Aspergillus oryzae, Mucor heimalis). Farm yard manure (FYM) and poultry manure (PM) were used as organic sources of nitrogen. Bio-primed (seeds soaked in 10% BM aqueous solution for 30 minutes just before sowing) and unprimed wheat seeds were tested under various nitrogen sources (NS) with and without BM. Nitrogen sources were NS1 (cont...

Muhammad Medrar Hussain*, Asad Jan* and Sayyar Khan Kazi 

...n: justify;">Rice (Oryza sativa L.) is a commonly consumed crop nourishing more than half of the human population. Different environmental stresses greatly affect cash crops. Among these, drought is a serious abiotic stress effecting rice productivity. Therefore, the current study was designed to know the role of OsTZF8 gene in conferring tolerance to drought stress. For this purpose, OsTZF8 gene was over-expressed in Oryza sa

Arshad Farooq1*, Muhammad Zafarullah Khan2, Abdul Hassan1, Muhammad Ishaq3 and Asif Nawaz

...erview schedule from 285 sample respondents randomly selected from eight villages of the eight selected union councils. The empirical results indicated that farmers of district Mardan had 41 percent knowledge gap with cane yield 58387 kgs/hectare and sample farmers of district Dera Ismail Khan had 33 percent knowledge gap with 82184 kgs/hectare. The results showed that in both districts sugarcane farmers had medium knowledge...

Muhammad Younis1, Shahen Shah1*, Inamullah1, Rozina Gul2, Arshad Jalal1, Farhan Khalil1, Iqbal Hussain1 and Muhammad Adnan Fahad

...uite conducive for the sesame crop production. However, area allocated and average production is still far below than the other countries of the world. One of the reasons of its low productivity in Pakistan is the low, inadequate and improper fertilizers management. Among the various factors influencing crop production, phosphorus (P) and sulphur (S) boost its yield and quality. A field trial was conducted in summer 2012 using phosphorus @ of 0, 25, 50 and 75 ...

Muhammad Salim1*, Ayhan Gökçe1, Muhammad Nadir Naqqash1 and Orkun Ersoy

...rose with increases in dosage and length of exposure. Significantly higher mortality (89.75±3.66%) after one week occurred in grains treated with the highest concentration (2000 mg/Kg). At the highest concentration (2000 mg/Kg), the mortality reached as high as 100% after 2 weeks, while the lowest significant percent mortality (1.00±1.00%) was recorded in the control one week after exposure and only increased to 3.53±0.00% after 3 weeks. P...

Filza Fatima Rizvi1, Muhammad Umair1, Muhammad Kahlid Jamil2*, Masooma Hassan2, Komal Qayyum1 and Syed Mohsin Raza Kazmi

.... Oil, gas and coal. The same is true for Pakistan that has agriculture based economy. The present study was undertaken to review energy consumption in the agriculture sector of Pothohar region of Pakistan. This region has no proper irrigation system due to its topography. Additionally, potential of renewable energy in Pothohar region that includes solar, wind and biogas has been calculated from the data, taken from different sources. Based on the analysis, it...

Gulnaz Parveen1*, Salma Gul2, Muhammad Ather Rafi3, Ashfaq Ali Khattak4 and Hikmatullah Jan

...ll test fungi included Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina and Rhizoctonia solani was significantly controlled by leaf powder (LP) and shoot powder (ShP) when applied alone and with combination. While combinations of 3% LP + 3% ShP, 3% LP + 5% ShP, 5% LP + 1% ShP, 5% LP + 3% ShP, 5% LP + 5% ShP completely controlled the infection of Macrophomina phaseolina. Similarly combination of 5% LP + 5% ShP com...

Muhammad Ali1*, Norsida Man2, Farrah Melissa Muharam2 and Siti Zobidah Omar

...rough multistage cluster sampling technique. Multiple linear regression through SPSS was administered for statistical procedure. The findings confirmed that the theory (TPB) was statistically feasible to predict behavioral intention of farmers to use ICTs for agricultural risk management. The results further reveal that attitude was one of the most influenced of intention, which was followed by perceived behavioral control and lastly the subjective norms. Thus...

Jaffar Hussain1*, Syed Farman Ali Shah1, M. Zeenat Ali1 and Javad Ahmad Shah

...ical properties of soil, samples were collected from different area of Jamshoro (Soman, Murad, Abadnear Kotrai Jamshoro). These nutrients are so important for crops yield and human being. The results explain that soil have low organic matter, pH is near to 7, copper content ranged was 5.82 - 8.2μ g/g, Maganese 6.7 - 8.3 μg/g and Zinc had values of 0.12 to 1.2μ g/g respectively. The conclusions are that the values of Zn, Fe, Cu and Mn are adequate. If ...

Bilal Saeed Khan1*, Muhammad Afzal2, Muhammad Asif Qayyoum1, Imran Ali3 and Abdul Ghaffar

...were also collected from same collection data and seven were from Muzaffargarh. 

...

Aurang Zeb, Abdur Rab and Shujaat Ali* 

...s i.e. location-1 (Torwarsak, Buner), location-2 (Rabat, Lower Dir), location-3 (Wartair, Malakand) and location-4 (Palai, Malakand) stored up to 48 days at room temperature, Data was recorded after 12 days of intervals. All the studied parameters were found significantly affected by both the factors i.e. storage duration and locations. The results revealed that the maximum fruit weight loss (16.37 %) was recorded from Wartair (Malakand). The percent weight l...

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Mohammad Farooque Hassan2, Sultan Ahmed Maitlo3 and Mukhtiar Ahmed Shaikh

...n sustainable pesticide usage in Pakistan. Randomly 66 farmers were selected to conduct the interview for background information about the growers’ status on pesticide application. The results show that the majority of the growers did not get any kind of trainings on sustainable use of pesticides. Results indicated that, 46.96% vegetable growers belong to middle age groups between 29-38 years. Most of them 40.90% possess low literacy up to primary and 27...
Mian Adnan Kakakhel1, Faryal Gohar1, Zahid Anwar2, Raza Ullah1, Muhammad Attaullah3, Shahbaz Ahmad4, Aamir Khan5, Kalimullah5*
... hundred number of blood samples (72 males and 28 females) were collected from Peshawar, Pakistan. The serum samples were analyzed for IgG and IgM by using enzyme-linked immunosorbent assay (ELISA), and statistical analysis carried out. Out of these tested

Ayesha Zafar1, Ghulam Sarwar1*, Muhammad Sarfraz2, Muhammad Zeeshan Manzoor1, Sher Muhammad3 and Ghulam Murtaza

... Pakistan. The plants in saline environment are negatively affected due to several issues like low osmotic potential, specific ion effect, and nutritional imbalance. The fertilizer behavior in salt stress conditions is quite different as compared to the normal soils. Linseed is an important oil seed crop. Its 50 % yield reduction occurs at ECe 5.9 dSm-1 and ESP value of 25-30. A field experiment was conducted on
Muhammad Mohibullah1*, Mehran1, Sundas Batool1, Muhammad Amin1, Zakiullah1, Muhammad Ilyas2, Irfanullah1, Abdur Rehman1 and Sardar Ali3
...ickpea genotypes for the said parameters. Overall, the gcv and pcv were low to moderate for majority of the parameters. Highest amount of gcv and pcv were recorded for seed yield per plant (30.48 and 32.00%) and secondary branches per plant (20.47 and 24.29%), respectively. High broad sense heritability and genetic gain values were also noted in seed yield plant-1 and 2ndry branches plant-1 ranging from 71 to 91% and 36.00 to 6...

Iram Fatima1*, Imran Pasha1, Ambreen Saddozai2, Shahid Nadeem3, Amer Mumtaz4 and Saqib Jabbar4 

...t role in the society by satisfying hunger at affordable rate for poor slums. This low infrastructure business is rising day by day due to growing urbanization. The study aimed at exploring the safety status of various snacks and beverages sold in the streets of Faisalabad, Pakistan. The three zones of city were selected for the purpose of

Wajiha Anum1*, Liaquat Ali1, Umair Riaz2*, Abid Ali1, Nadia Manzoor3, Laal Hussain Akhter4, Asad Ur Rahman5, Naeem Maan6 and Ijaz Ahmad

Muhammad Rasheed1*, Zafar Ullah1, Muhammad Ansar1, Asma Hassan1, Shahzada Sohail Ijaz2, Muhammad Hussain Shah4 and Muhammad Arshadullah

...and yield of oats (Avena sativa) and vetch (Vicia sativa) grown in mixture stands under conservation and conventional tillage systems. The highest number of tillers plant-1 (11) for Oats was recorded in oats-vetch mixture under conventional tillage (CT) while comparing minimum tillage (MT) to zero tillage (ZT) 43 percent higher yield of oats was obtained under MT. The impact of cereal legume mixture on forage yield was optim...
Neelam Pery*, Nayab Batool Rizvi, Muhammad Imtiaz Shafiq
...with better efficacy and safety profile. However, certain factors such as lack of universal response, the need for prolonged therapy, subcutaneous or intravenous mode of administration along with high cost have rendered these unattractive. All this has paved the path for orally available small molecule kinase inhibitors and ‘Tofacitinib’, the first FDA approved Janus kinase inhibitor, invaded the market. The succ...
Majeeda Rasheed1*, Tanveer Akhtar1, Nadia Mukhtar2, Muhammad Furqan Shahid2, Muhammad Imran3, Saima Yaqub2
...Open-Epi software. Serum samples including goats n=500 and sheep n=500 were collected from different herds situated at Naltar lake, Tattovat, Fairy meadows, Bangle, and Naltar. Overall a comparable prevalence was identified for both goat and sheep (46% vs 44%, P > 0.05). Future studies are necessary to further ascertain the study outcomes and elucidate the molecular epidemiology of prevalent strains in the
Saira Saleemi1*, Zafar Iqbal1, Abdul Nasir Khalid2
...recorded on all 200 fish samples. Fins were the most affected parts with 39.10% infection. Intestine was the least affected part with 6.59% infection. The major reason of aspergillosis in silver carp is the use of unhealthy and unhygienic feed given to silver carp reared in earthern ponds. Due to aspergillosis fish may not be recommended for human consumption. If proper health management practices are adopted on fish rearing facilities, then the chances of inc...
Hina Nazli, Abida Butt*
...s. The results of both assays showed that male spiders were more susceptible than female spiders in both acute and residual toxicity tests. The residues study data showed that spiromesifen is short- lived (< 5 days aged residues cause < 30% mortality) and that thiamethoxam is slightly persistent ( 5–15 days aged residues cause < 30% mortality). 
Ayesha Iqbal1, Ghulam Sarwar1, Abdul Majid Khan1*, Muhammad Tahir Waseem1, Ayesha Iqbal1, Rana Manzoor Ahmad1,2, Muhammad Ameen1
...l direction and curve dorsally. The estimation of suid’s ages was based on third molar eruption. The fifty-one craniometric and twenty mandibular measurements were carried out on the adult male skull and data derived from analysis of our sample was compared with previously reported data of European and Japanese wild boar. As a result of comparison of the different craniometric and mandibular parameters it was inferred ...

Zubair Aslam and Ali Ahmad* 

... ve Ltd. Şti. Suleymanpasa, Tekirdag, Turkey under field conditions. In field four different states of fertilizers [F0: Control (No fertilizer application); Fvc: Solid vermicompost @ 5 ton/ha; Fvt: vermi-tea @ 6 %; Fc: Chemical fertilizers (N and P) @ 120-100 kg/ha were applied in maize hybrid P30B74. The plants were harvested 45 days after sowing and the evaluation was done on the basis of various morphological (root length, shoot length, root fresh weight, ...

Korkmaz Bellitürk1, Zubair Aslam2, Ali Ahmad2* and Sami ur Rehman2

...as the test species. The samples were collected from prepared vermicompost and raw material for chemical and physical examination. For these samples, the pH, EC, organic matter, C:N ratio, phosphorus, potassium, calcium, magnesium, iron, nitrogen, sulfur and heavy metals i.e. tin, cadmium, nickel, lead, mercury and chromium parameters were evaluated. The experimental design was Complete Randomized Design (CRD). The data obta...

Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider 

...er studies are much necessary to ascertain genetic characteristics of CDVs over a wide geographical area of the country together exploring the potential reasons of vaccine failure.  

...

Anurag Sharma*, Naresh Kumar, Geetanjali Singh and Anika Sharma 

...s suggests that they are safe and effective, however, scientific validation is still required for many of them especially for animal use. The phyto-pharmacological research on these two natural products show a ray of hope for the discovery of new active compounds with a novel structure that has potential to serve as a natural lead compound for the development of new nutraceuticals and galactagogue for animal health. 

...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...e era appears to be necessary in order to identify unusual rotavirus strains that might not be fully contrasted by current vaccines. 

...

Zia-Ur-Rehman1, Faisal Sohail Fateh2*, Humayun Saleem1 and Muhammad Abu Bakar Siddique1 

...tan. It belongs to the Rosaceae family. In Pakistan, peach with production of 72536 tonnes is grown on an area of 14350 hectares. A variety of diseases infect the peach tree. Among these diseases, brown rot disease is very important that reduces yields primarily by decaying the fruits on the tree and after harvest. A preliminary study was designed to see the prevalence, incidence and severity of peach rot disease in the markets of Federal Territ...

Muhammad Abu Bakar Siddique1, Faisal Sohail Fateh2*, Zia-Ur-Rehman1 and Humayun Saleem

Hafiz Abdul Ghafoor1*, Muhammad Afzal1, Muhammad Luqman2, Muhammad Arshad Javed2, Syed Wasim Hasan3 and Muhammad Zeeshan Majeed

...iptera) is a destructive sap-feeding pest of a number of horticultural and agricultural crops in Indo-Pak region. It has been an emerging threat to citrus industry in Pakistan and is considered as hard-to-control pest. This study evaluated some selected pest control options against the infestation of D. mangiferae under field conditions. The treatments included some most effective conventional and novel chemistry insecticides, botanicals and cultural control p...

Muhammad Raheel Faiz1, Ijaz Ashraf1, Haq Nawaz2, Muhammad Zubair3*, Muhammad Sajid Mahmood4, Sajid Hussain5 and Abdul Qadeer2 

...g possible solutions. Faisalabad Sadar Tehsil was selected as a study area where the vegetables are intensively grown by the farmers. There were total 58 rural union councils in Faisalabad Sadar, out of which 6 were selected through simple random sampling. From each selected union council, one village was selected at random and from each selected village, 20 vegetable growers were selected...

Khalid Khan1, Marguerite Wotto2, Saima Liaqat3, Gulawar Khan1, Balach Rasheed1, Sara Rafiq4 and Guo Xiangyu5* 

...wers. Multi-stage random sampling technique was used for selection of the farmers. The Cobb-Douglas production function was used to estimate the technical efficiency and performance of the farmers. The study results disclosed that all explanatory variables have a positive impact on the farmers’ technical efficiency apart from pesticides. Additionally, the average technical performance of the farmer was 85% which shows that on average a farmer can spur th...

Hafiz Muhammad Imran Javed* and Mushtaq Ahmed 

...e conserved regions were same regardless of the gene while the substitutions were seen at different residues other than the conserved domains. However, the analysis based upon the three-dimensional protein structures revealed that the study isolates showed mutation at C-terminus region of N protein. Such a study helps to improve our understanding towards evolution of emerging and re-emerging strains of SRMVs in the country. 

...

M. Hammouchi1*, G. Sebbar1, N. Touil2, C. Loutfi1, Y.S. Malik3, M. El-Harrak1 and O. Fassi-Fehri4 

...table in swabs and fecal samples taken at the peak of infection. The transmission of the disease to animals (Timahdid breeds) brought into contact without manifestation of clinical signs, may indicate that local breeds of sheep might play an important role in the prevention of dissemination of PPR by virtue of its natural immunity. 

...

Pravin Mishra1, Md. Muket Mahmud2, Md. Ahosanul Haque Shahid2, Alamgir Hasan2, Vivek Kumar Yadav3 and Moinul Hasan1* 

 Salman Khan, Irfan Ullah*, Shahid Ali and Murtaza

...an. A three-stage random sampling technique was used to collect data from 100 respondents during 2018. A Cobb-Douglas type production function was used to approximate effect of determinants on potato yield. Major shares of the input cost per acre incurred in the study area were seed (Rs. 18720), followed by land rent (Rs.15890) and labors having an estimated cost of Rs. 14965 respectively. The analysis showed that on average per acre yield of potato was record...

 Abdus Salam* and Muhammad Zafarullah Khan

...Based on the multi-stage sampling technique, population for the current study, 3 zones from 5 were purposively selected on the basis of their different agricultural condition. Selection of sample size was made using Yamane formula. The number of respondents as per formula was confined to 188 respondents in district Mansehra, 153 respondents in district Swabi and 59 respondents from district Karak, where data were collected r...

Abdul Raqeeb1,3, Syed Moazzam Nizami2,4*, Aamir Saleem3, Lubna Ansari3, Saeed Gulzar3, Basit Ali3, Masooma Saleem5
 

...rowing area. Destructive sampling including excavation of roots was carried for overall 60 trees. The results revealed generic equations for above ground biomass and volume. However, statistical analysis pointed out several significant differences in biomass allocation among C. deodara tree due to stand characteristics, highlighting the role and importance of microenvironment in regional and national forest inventories. The study provides a comprehensive tool ...

Javed Iqbal1, Ali Zohaib1*, Muzzammil Hussain1, Adnan Bashir1, Muhammad Hamza2, Wardah Muzaffer3, Muhammad Tahir Latif1 and Naeem Faisal1 

...nts. Wheat varieties (Faisalabad-08, Punjab-11 and Galaxy-13) were sown on ridges using two different seed rates (125 and 150 kg ha-1). Increase in seed rate improved the emergence (plants per m2), number of productive tillers per m2 and grain yield (3-7%) of all wheat varieties although productive tillers per plant, number of grains per spike and 1000-grain weight was decreased. Faislabad-08 performed better regarding grain yield and related components among ...

Ehsan-Ul-Haque1, Akbar Hayat1*, Muhammad Asim1, Sajjad Hussain2, Muhammad Shakeel Hanif3, Muhammad Zubair1, Muhammad Abdullah Jamil1 and Faheem Khadija1 

.... Various fungicides and salts are incorporated in wax to limit postharvest fruit decay and fungal attack. In the present study two salts were incorporated in commercial wax to control postharvest decay in Willow leaf mandarin (Citrus reticulata Blanco) and Kinnow mandarin (Citrus reticulata Blanco). Sodium bicarbonate and Potassium sorbate incorporated (6% w/v concentration) in wax were used to compare with commercially use...

I. Erum, K. Nasira, H. Sagir, S. Raza and Firoza, K.

Description of Discolaimus miniodontii n.sp. and Laevides hunderansis n. sp. with notes on Discolaimoides spatilabium (Nematoda: Dorylaimida) from District Ghizer, Gilgit-Baltistan, Pakistan
...xt-align: justify;">Soil samples collected from District Ghizer, Gilgit, Baltistan, Pakistan, yielded two new and a known nematode species of the order Dorylaimida viz., Discolaimus miniodontii n. sp. and Laevides manilkarii n. sp.; and a new report of a known species Discolaimoides spatilabium from District Ghizer, Gilgit-Baltistan, Pakistan. Discolaimus miniodontii n. sp., is characterized by having smallest odontostyle (9-10µm); relatively smaller and...

A.N. Ashfaque1, F. Shahina1 and S. Dawar2

 

Diversity of nematode fauna associated with cotton fields of high temperature areas of Sindh, Pakistan

M. Rufai1† , A. A.Wahab2 , Q. O. Adeshina1 , J. C. Umehokoh1 and O. O.Ologunro1

Occurrence of entomopathogenic nematodes in Osun State, Southwestern Nigeria
... pH. A total of 110 soil samples were randomly collected from various cultivated fields in different locations. The soil samples were baited twice using last instar larvae of the Galleria mellonella (greater wax moth) for presence of entomopathogenic nematodes. Four EPN isolates were recovered viz., Heterorhabditis indica, Heterorhabditis bacteriophora, Steinernema karii and Steinernema wesieri. Of a total of 110 soil

S. Ahmed† , L. Yue, Q. Liu and H. Jian

Evaluation of media for mass production and fermentation of Pseudomonas spp. for integrated management of cereal cyst nematode (Heterodera avenae)
...ried results on the bioassay against the second stage juvenile mortality (J2) of H. avenae. The culture filtrates formed in media No.8 (Corn flour 17g/L, Glucose 10g/L, Soybean Cake Powder 25g/L, Maltose 10g/L, Yeast Extract 5g/L) and media No.5 (Corn flour 5g/L, Yeast Extract 1.5g/L, Fish Peptone 2.5g/L, Soybean Cake Powder 20g/L, Peptone 0.75g/L, KH2PO4 3g/L, CaCO3 0.5g/L, MgSO4 0.3g/L) has more toxic effects on the second stage juvenile (J2) mortality as co...

A. S. M. El-Nuby1† , S. A. Montasser2 and I. A. El-Khadrawy

Control of root-knot nematodes using wild plants colonized Sinai, Egypt

 K. Abbasi 1 , R. Wick2† and D. Zafari1

Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis
...itin by the N-acetylglucosamine-dinitrosalicylate method. Ten isolates with high chitinase activity were selected for further characterization. Of the 10 selected isolates, three chitinase enzymes were evaluated using three substrates. Based on the results of our chitinase assay, Beauveria bassiana, Lecanicillium muscarium, Paecilomyces sp. and Trichoderma atroviridae had the highest activ...

K. Osei1† , A. I. Adama1 , E. C. Tagoe2 and J. Sackey-Asante1

 

Biochar effect on nematodes and insects population density, soil improvement and yield of okra
...nts to okra production (Asare-Bediako et al., 2014b). The attack of root-knot nematodes (Meloidogyne spp.) has been reported as the most serious, widespread and alarming which causes tremendous yield losses (Hussain et al., 2011; Kayani et al., 2013; Barros et al., 2014). Flea beetle (Podagrica spp.) is the most important insect pest of okra in Ghana and Nigeria (Obeng-Ofori & Sackey, 2003; Asa

 M. M. A Youssef and W. M. A. El-Nagdi †

Effect of some temperature changes on the population density of some plant parasitic nematode species

Sahib Khan1, Muneeza Wahid2, Muhammad Abeer Irfan1, Muhammad Naeem3, Asma Gul4, Muhammad Haseeb Zafar2

ROW LEVEL IMAGE FORGERY DETECTION TECHNIQUE USING EMBEDDED DIGITAL SIGNATURES
...tal signatures. Using message digest 5 algorithm, digital signature is generated from selected pixels of each row using selected pixels for and embedded in the least significant bits of selected pixel of the corre- sponding row of digital image. The proposed technique is powerful enough to detect different image manipulations. The results show that it can successfully detect one least significant bit alteration made in any pixel of digital image.

...

Sahibzada Abdur Rehman Abid1*, Muhammad Naeem2, Asma Gul3, Nasir Ahmad1 

PASHTO OPTICAL CHARACTER RECOGNITION USING NEURAL NETWORK
...aracters with
110 samples for each Pashto character has been used to train the network.

...

Muhammad Alam Zeb Khan1*, Muhammad Ali Kamran1, Khan Muhammad2, Khizar Azam1 

IDENTIFICATION OF THE OPTIMAL OPERATING PARAMETERS OF LOCALLY AVAILABLE COAL FOR USE IN PAKISTANI INDUSTRIES
...being reported. The coal samples were tested in a cyclone combustor using the pulverized coal
combustion technique. Effect of mesh size, primary and secondary air velocity at various air fuel ratios was investigated
to quantify the combustion performance by measuring the furnace temperature. Mesh size 200 was found to
be readily combustible within the operating ranges, while tight control of the operating parameters was required
to ...

Mahmood Alam Khan1, Muhammad Yaseen1, Mujahid Khan*2, Amjad Khan1 

TEMPORAL VARIATION IN GROUND WATER QUALITY DUE TO URBANIZATION. CASE STUDY: DISTRICT PESHAWAR, PAKISTAN
...hority (PSQCA). In 2005, same study for district Peshawar was carried out jointly by PCSIR Peshawar and
Kyungpook National University. In order to assess the effects of urbanization on ground water quality in district
Peshawar, another study was undertaken in 2014-15 by collecting samples from the same locations. Standard procedures
were adopted to determine the select...

Feroz Shah1, Mohammad A. Irfan1, Kareem Akhtar1, Abdul Shakoor1, Naeem Khan2

EFFECT OF POROSITY ON MECHANICAL PROPERTIES OF DIE CAST ALUMINUM ENGINE BLOCK
... investigate porosity in samples taken from different
locations of the engine block, i.e. thick and thin sections. The samples were extracted from thick and thin sections of
the engine block, having section thicknesses of 20 mm and 5 mm, respectively. Mechanical testing of these samples
was carried out to show effect of section thickness and its associated porosity on ...

Riaz Muhammad1*, Naseer Ahmeda, Abdul Basit2, Rizwan Alim Mufti2 

EXPERIMENTAL INVESTIGATION OF FRACTURE TOUGHNESS, HARDNESS AND LOAD-INDENTATION DEPTH RESPONSE OF TI-64 USING VICKERS INDENTATION TECHNIQUE
... 50 percent of titanium usage in today
modern world high-tech industries subjected to various nature of cyclic loading. This study includes the experimental
assessment of fracture toughness of Ti-64 using indentation technique which is easy and fast experimental techniques.
For this purpose Vickers-indentation technique is employed to report the hardness followed by fracture toughness
evaluation of the studied alloy using indentatio...

Zeeshan Al Hameed1, Junaid Saleem2*, Syed Sajid Hussain1, Ahsan Abdul Ghani2, Hira Lal2 

STUDY OF INDIGENOUS FLUORSPAR AS METALLURGICAL FLUX
...around one third of its usage is in the form
of metallurgical flux, a key slag fluidizer in steel industry as it increases the fluidity of slag in the ladle furnace.
However, it needs to undergo beneficiation process to get it concentrated before being added into the furnace. In this
study, we report, for the first time in Pakistan, that the indigenous Fluorspar can directly be used as a metallurgical
flux without any beneficiation ...

Zulfiqar Ali Soomro1* 

NOISE CONTROL FILTRATION OF LATERAL AND YAW DYNAMICS FOR RAILWAY VEHICLE WHEELSET ON TRACK
... is used framed for necessary modeling. The estimation
of perturbations for the movement by wheels and velocity of train in lateral and yaw phases are enumerated.
Here dual bucy kalman estimator is implemented to decrease the influence of the noise caused due improper ratio
of adhesion level upon track. One estimator reduces the overshoot of noise and other to minimize it at lower level
of level of error. Further the behavior of lat...

Younis Jamal1, Asad Naeem Shah1, Wasif Amin Butt1, Ahmad Naveed1*, Muhammad Usman1 

EXPERIMENTAL INVESTIGATION OF THE EFFECTS OF FUEL INJECTION PARAMETERS ON DIESEL ENGINE PERFORMANCE AND EMISSIONS
... nozzles
known as sac and valve covered orifice (VCO) were used with hemispherical cavity and toroidal cavity pistons,
respectively. Besides an already existing set of sac type nozzles, six distinct combinations of nozzles with varying
cone angles and tip penetration (protrusion) lengths (designated as 135° × 3.5 mm, 140° × 3.5 mm, 145° × 3.5 mm,
150° × 3.5 ...

Daulat Khan1*, Khizar Azam2, Noor Muhammad Khan1, Muhammad Israr1, Naeem Ejaz3 

ASSESSMENT OF WASTEWATER FOR DISPOSAL IN ACCORDANCE WITH NEQS CRITERIA AND ITS REUSE FOR IRRIGATION WITH DILUTION
...tigations on 5 composite samples were carried out. Total of 19 parameters were analyzed
including Total Dissolve Solid (TDS), Total Suspended Solids (TSS), Bio-Chemical Oxygen Demand (BOD), Chemical
Oxygen demand (COD), Sulfide, Fluoride, Lead, Nickel, Boron, Copper, Iron, Zinc, Manganese, Chlorine Total and
Chromium hexavalent with average value of 502.6, 665.6, 267.4, 453.8, 3.1,5.3 0.6, 0.4, 1.4, 0.5, 0.5, 2.15, 0.6 and
0.5 mg/L ...

Samreen Fatima1*, Mudassir Uddin1 

COMPARISON OF ASYMMETRIC GARCH MODELS WITH ARTIFICIAL NEURAL NETWORK FOR STOCK MARKETS PREDICTION, A CASE STUDY
...el was developed for the said stock markets. Lastly, forecasting performance of the
FA-GARCH and ANN models both in and out sample were evaluated using root mean square error. In the A-GARCH;
EGARCH (1,1) performed better than PGARCH(1,1) in both stock market data. However, when comparing A-GARCH
with ANN, it was found that ANN gave minimum out sample forecasting error...

Shafiullah1*, Mohammad Nafees2 

MUNICIPAL WASTEWATER TREATMENT BY COAGULATION/ FLOCCULATION PROCESSES VIA OPTIMIZATION OF INOCULATION DOSE AND SETTLING TIME
...hree months. In total 45 samples were collected from five different sites at the
rate of three samples per day (Morning, Afternoon and Evening). Each sample was a composite of three grab samples
collected with four hours intervals. All Samples were analyzed for physico-chemical characteristics. On comparison
with Pak NEQS it was obse...

 M. Ihsan Ul Haq1, Ahmad Hussain2*, Farzana Yasmeen3, Shafiq R. Qureshi4

LIFE CYCLE COSTING AND PAYBACK PERIOD EVALUATION OF A SOLAR THERMAL DESALINATION SYSTEM
...2025.Non availability of safe drinkable water is the major source of diseases in the different regions of the world
especially remote rural and coastal areas. About 97% of water on earth is comprised of seawater. Desalination of
saline water is a prominent approach to handle the problem of water scarcity. Conventional desalination technolog...

Muhammad Aamir1*, Riaz Muhammad1, Naseer Ahmed1, Muhammad Sadiq2, Muhammad Waqas3, Izhar1 

MECHANICAL PROPERTIES OF LEAD FREE SOLDER ALLOY FOR GREEN ELECTRONICS UNDER HIGH STRAIN RATE AND THERMAL AGING
...train curves using universal testing machine (UTM). Results show
that LFS alloys are extremely sensitive to changes in both temperature and strain rate. The microstructure becomes
coarsen after thermal aging due to growth of average IMCs particle size which significantly results in reduction in
YS and UTS. Furthermore, increasing strain rates results in increasing YS and UTS due to less creep deformation.
Mathematical relations are ...

 Waqas Javaid1*, Adnan Tariq1, Iftikhar Hussain2, Sahar Noor2

AN EVOLUTIONARY APPROACH FOR CMS DESIGN
...al effort. Following the same trend an evolutionary algorithm has been developed
during this research by combining Standard Genetic Algorithm with a very effective Local Search Heuristic (LSH).
The results show that it is efficient both in terms accuracy and speed of convergence (CPU time). 

...

Muhammad Naeem1*, Sahib Khan2, Nasir Ahmad3

A DISTRIBUTED GENETIC ALGORITM AND A-PRIORI ALGORITHM FOR THE HUB AND FACILITY LOCATION PROBLEMS

Khizar Azam1*, Abdul Shakoor1, Shahid Maqsood2, Muddasar Habib3, Muhammad Fahad4, Akhtar Zaib1 

IDENTIFICATION OF SITES FOR ESTABLISHING PHOTOVOLTAIC SOLAR FARMS IN PAKISTAN USING SOLAR GEOMETRY

Rafiullah Khan1, Waseem ur Rahman1, Misbah Ullah2, Kamran Afaq3, Muhammad Amjad1, Sakhi jan1 

AGE EFFECT ON THE MECHANICAL PROPERTIES OF HIP JOINT BONE: AN EXPERIMENTAL INVESTIGATION
...mposite, composed of bonesalt
plates as matrix binder and soft collagen as reinforcing fibers. As bone supports all the organs of a body so its
mechanical behavior is of prime importance. The mechanical properties of bone varies with age. In this paper, the
effect of age on various mechanical properties of hip joint bone was investigated experimentally. For this purpose
hip joint bone specimens of male bovine of different age groups...

Bibi Safia Haq1,2, Hidayat Ullah Khan3, Abdul Shakoor4, Shahnaz Attaullah5, Ishrat Rahim1 

PROJECTION ETCHING OF KERATIN THIN FILMS FOR FABRICATION OF SKIN STRUCTURE USING ARF EXCIMER LASER
...e slower. Keratin
samples with different film thicknesses were ablated with different pulses. The depth of the ablated keratin layers with
different pulses were also measured by scanning the sample using a Dektak KLA-Tencor and the surface statistics of
the ablated keratin sample have been analysed using a white light interferometer WYKO NT1100. 

...

Bibi Safia Haq1,2, Shahnaz Attaullah3, Abdul Shakoor4, Hidayat Ullah Khan5, Khan Alam5, Kausar Shaheen1 

CHARACTERISTICS AND EFFICACY OF ULTRAFAST LASER PULSES FOR BIOMEDICAL APPLICATIONS
...en demonstrated that Ti: sapphire laser system has the ability to fabricate three dimensional structures for biomedical
applications. Tissue scaffolds have been prepared with different pore sizes suitable for application in different
living tissues. Polymerisation based on a direct write system to build up solid polymeric material for the fabrication
of three dimensional cell scaffolds can be achieved. B...

Khuram Rashid1, Saroosh Danish2, Muhammad Akram Tahir3 

EXPERIMENTAL INVESTIGATION ON UTILIZATION OF WASTE RUBBER TIRES AS FILLER IN CONCRETE
... evaluate the potential usage of waste rubber tires in concrete. Disposal of
tires is a major problem and their accumulation create health and fire hazards. To incorporate waste rubber tires in
construction industry is considered as a sustainable and environment friendly solution. Experiments were designed
by incorporating coarse and fine rubber content as partial substitution of conventional aggregates....

Nazish Huma Khan1, Mohammad Nafees1, Adila Bashir1, Farooq Ahmad1 

STUDY OF POLLUTION LAOD IN PAPER MILL EFFLUENTS AND ITS RECYCLING BY SUNDRY PLANTS AT HAYATABAD INDUSTRIAL ESTATE, PESHAWAR
...amined for operation and sampled for waste water. Total 7 waste water samples were collected from selected
points of Paper mills, Industrial drain and Sundry plants. Samples were analyzed for various physicochemical characteristics (colour, pH, EC, Alkalinity, Turbidity, TDS, TSS, COD and BOD5) by adopting standard methods for the
examination of water and waste water. These sa

Shabana Rafique1, Shahnaz Parveen Khattak1, Tanveer Hussain2, Faiza Tauqeer1, Bashir Ahmad3 

EVALUATION OF FLEXURAL RIGIDITY AND ABRASION RESISTANCE OF POST AND META-FINISHED PIGMENT DYED P/C FABRICS
...an be conserved by using same machinery for dyeing, finishing, drying and curing. Furthermore, elimination
of post wash treatment in this method caused little or no contamination. Despite its manifold advantages, the rubbing
fastness and fabric stiffness of pigment dyed fabrics in deeper shades is doubtful, hence needed further exploration.
The effect of various parameters i.e. finish type and concentration were taken in to consideration a...

Farid Ullah Khan1, Muhammad Iqbal1 

DEVELOPMENT OF A TESTING RIG FOR VIBRATION AND WIND BASED ENERGY HARVESTERS
... testing are the most versatile, inexpensive
and easy to operate, however, due to their fixed displacement, single frequency and sinusoidal behavior, usage of
these shakers is limited. The less known electro-hydraulic shakers are more robust, but due to their high forces and
high velocities, these are usually utilized to characterize heavy samples. Electromagnetic shak...

Muhammad Masood Tahir1, Ayyaz Hussain2, Saeed Badshah3, Qaisar Javaid2 

RULE-BASED IDENTIFICATION OF BEARING FAULTS USING CENTRAL TENDENCY OF TIME DOMAIN FEATURES
...ng the unknown vibration samples. The results are evident that the proposed
RDS may be an effective alternative to the existing classifier-based fault diagnosis, even if the vibration signals are
contaminated with considerable background noise. 

...

Salman Saeed*, Akhtar Naeem Khan**, Rashid Rehan*, Sikandar Hayat Sajid**, Muhammad Sagheer Aslam*, Khan Shahzada** 

COST AND PERFORMANCE OPTIMIZATION OF PRECAST POST TENSIONED PRE-STRESSED GIRDER BRIDGE SUPERSTRUCTURES
...ture
are not necessarily competing factors. Relationships for slab thickness and girder spacing are presented that result
in optimal performance and cost of the bridge superstructure. 

...

Faisal Shabbir*, Naeem Ejaz*, Daulat Khan**, Naveed Ahmad*, Jawad Hussain*, Muhammad Fiaz Tahir* 

INVESTIGATION OF USING PAPER INDUSTRY WASTE (HYPO SLUDGE) IN CONCRETE MIX
...esources
(gravel, sand, fine rock, water etc). In order to address such environmental issues related to cement manufacturing
there is dire need of replacing cement with other binders to be used in concrete. This study investigates the utilization of waste paper sludge ash (WPSA) in concrete. WPSA was part...

Rahim Haider*, Al Mustaan Kakar*, Sikandar Bilal Khattak*, Safi ur Rahman**, Shahid Maqsood*, Misbah Ullah*, Rehman Akhtar*, Aamir Sikandar* 

DEVELOPMENT OF OPTIMIZED MAINTENANCE SYSTEM FOR VEHICLE FLEET
...lity and customer
satisfaction. The major cause for delays is inefficient and ineffective maintenance procedures. To avoid major market
losses, an efficient maintenance model is required. This research proposed a maintenance system that integrates the
flow of information by incorporating different maintenance strategies. The analysis is carried out on the basis of
likelihood of the failures. Five Why Analysis is used to identify the...

Sahib Khan*, Abdur Rehman*, Nasir Ahmad**, Muhammad Naeem*** 

THE REDUCTION OF SPECIFIC ABSORPTION RATE AT DIFFERENT FREQUENCIES

Imran Shehzad1, Ghulam Sarwar1*, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Sher Muhammad2 and Ghulam Murtaza

...ulture production due to salinity is one of the key problems in many areas around the globe including Pakistan. Around the globe approximately, 800-million-hectare area is salt affected and it is estimated that up to middle of 21st century, 50 % of the arable land all over the world will be destroyed due to salinization problem. Use of canal water is the only strategy where

Gulzar Ahmad, Muhammad Inyatullah Babar, Muhammad Irfan 

A NOVEL WIDEBAND MICROSTRIP PATCH ANTENNA FOR SATELLITE COMMUNICATIONS IN KU-BAND
...na has been proposed for satellite communications. The bandwidth has been increased using parasitic patches. The size of the proposed antenna is 15x8 mm2. It has been observed that the antenna has a bandwidth of 4.08GHz, a return loss of 49.07 dB at the center frequency, a maximum gain of 8.25 dBi and total efficiency of more than 90%. This antenna can be prospective candidate for satellite communications.

...

 Mahmood Alam Khan, Afzal Munir, Muahmmad Shahzad Khattak, Daulat Khan, Muhammad Ajmal

GROUNDWATER RECHARGE QUANTIFICATION FROM RAINFALL IN PESHAWAR DISTRICT-KHYBER PAKHTUNKHWA
... and current pattern of usage are threats to human welfare, livelihood, development, and for the life itself, in the coming years. The main objective of this study was to quantify the ground water recharge due to rainfall on yearly basis of Peshawar district Khyber Pakhtunkhwa Province of Pakistan. Groundwater recharge was estimated by subtracting runoff from the rainfall. The Soil Conservation Service Curve Number (SCS-CN) method was used to estimate the runo...

Sajjad Hussain, Nisar Mohammad, Zahid Ur Rehman, Noor Mohammad, Ishaq Ahmad, Naseer Muhammad Khan, Salim Raza 

EXTRACTION OF GOLD AND SILVER BY CYANIDATION PROCESS FROM COMPLEX ORE OF SHOUGHUR AREA CHITRAL, KYBER PAKHTUNKHWA
...rs like grinding time, dosage of pH, cyanidation time, dosage of sodium cyanide (NaCN) and dosage of lime Ca (OH)2 were optimized. The samples were subjected to differential grinding time varying from 20 to 35 minutes, pH of slurry adjusted from 9 to 11 by adding Ca (OH)2 from 0.5 to 1.5 gm/kg , dosage of NaCN varies...
Hafiz Khurram Shurjeel1, Muhammad Anjum Aqueel2, Ejaz Ashraf3*, Asad Ali4 and Arooba Rubab5
Effect of Insecticides on the Longevity of Apis mellifera L. (Hymenoptera: Apidae)
...h frame were selected as samples for further studies. After egg hatching larval duration was recorded until pupal duration starts. The honey bees were caged after pupal duration starts and marked with permanent marker for adult data recording. The data was recorded daily for 30 days. The effects of different chemical insecticides (nitenpyram @75ml/acre- new chemistry, cyhalothrin; nok-out @200ml/ acre, tycon; emamectin benzoate @ 200ml/acre) on egg presence, e...
Kemi F. Omotesho*, Adedamola V. Adetayo, Adeniyi F. Akinrinde and Deborah A. Olabode
 
Challenges to the Adoption of Agricultural Innovations: The Case of Yam Minisett Technology in Kwara State, Nigeria
...vestigated. A four-stage sampling procedure was used to select 205 respondents on whom an interview schedule was administered. Descriptive statistics, Probit Regression Analysis and Pearson’s Product Moment Correlation, were used to analyse data. Result reveals that the farmers were mostly males (71.2%), middle-aged (50.7years,) with an average of 28 years of farming experience. Only 58.5 percent of the farmers had adopted the YMT. Training was most requ...

Inamullah Khan1*, Amjad Usman2  and Rahamdad Khan2

The Mortality Rate of Pupae and Adult of Fruit Fly Bactrocera cucurbitae Coquillett (Diptera: Tephritidae) Affected by Different Submerging Time and Soil Types under the Laboratory Treatment
...s (1, 2, 3 and 4 hrs) on same age pupae also showed similar trends in pupal’s mortality and emergence to adult stage.In further investigation on the effect of four soil texture classes such asclay loam, silt clay loam, sandy loam and pure sand as control, low emergence rate and high mortality was recorded from silt clay loam texture soil as compared to only s...

Shaista Naz1*, Noor P. Khan1, Himayatullah Khan1 and Azra2 

Does Women’s Participation in Livestock Management Enhance their Empowerment? An Insight from the Tribal Belt of Pakistan
...ucts for consumption and sale. However, the question arises that women’s participation in livestock management has some relation to their empowerment or it is not a debatable issue. To address the issue, this research is carried out in the tribal areas of Pakistan for which data were collected from 323 randomly selected female respondents from 323 randomly selected households in Mohmand Agency. The collected data were analyzed using percent participation...

Hina Fatima1*, Sania Shaheen2, Lal Khan Almas3 and Sehrish Haroon4

Profit Efficiency among Transplanting and Direct Seeded Rice Producers (Case Study of Certain Rice Growing Areas of Province Punjab, Pakistan)
...ned areas through random sampling. Stochastic Frontier Analysis (SFA) serves as a tool of obtaining reliable estimations of profit efficiency of rice production. Findings from SFA reveal profit efficiency of rice farmers for TRP to be 57% and 83% for DRS farmers. The results further Bring to light the loss of 43% profit of TRP and 17% profit loss of DRS due to unsuitable combinations of inputs. Direct seeded rice farmers displayed more efficiency compared to t...

Muhammad Zamin1*, Fazli Rabbi2, Shahen Shah3, Muhammad Amin4, Haroon Ur Rashid5, Hasnain Alam6 and Sabahat  Ali1

Performance of Lilium (Lilium elegans L.) Genotypes using Different Planting Media
... planting media viz., M1-sandy soil+ Compost (1:1; v/v), M2- sandy soil+Humic acid (20:1;v/v), M3- sandy soil+ Peatmoss (1:1;v/v), M4-sandy soil+Potting soil (1:1;v/v). in randomized complete block design (RCBD) with 3 replications. The lilium genotype Bomi Yellow performed well in terms of maximum plant height (72cm), highest number of flowers (5.4) and...

Sajjad Haydar*, Zunaira Asif*, A. A. Bhatti**, Obaidullah Nadeem***, Ghulam Hussain*, Nadeem Abbas*

EFFICIENCY OF WASTEWATER TREATMENT PLANT IN THE PUNJAB TANNERY SECTOR: A CASE STUDY OF DADA TANNERY
... was found deficient for satisfactory biological treatment. WWTP showed
overall removal efficiency of 88.81, 84.54 and 62.31% for total suspended solids (TSS), five day biochemical oxygen
demand (BOD) and chemical oxygen demand (COD), respectively. Whereas, the mean effluent concentrations of
TSS, BOD and COD were 216 mg/L, 199 mg/L and 1023 mg/L, respectively. The effluent from WWTP exceeded the
National Environ...

Ihsan Rabbi*, Sehat Ullah**, Aftab Alam**

MARKER BASED TRACKING IN AUGMENTED REALITY APPLICATIONS USING ARTOOLKIT: A CASE STUDY
...eal world object is necessary. The registration
is needed for tracking video camera pose (position and orientation) with respect to the real world objects. In
existing vision-based augmented reality systems, marker-based technique is widely used approach to track the video
camera pose. In this paper, we present an analysis of marker-based tracking using ARToolKit. We investigate the
effect of marker size, distanc...

 Muhammad Nafees*, Asim Nawab*, Wisal Shah**

STUDY ON THE PERFORMANCE OF WASTEWATER TREATMENT PLANT DESIGNED FOR INDUSTRIAL EFFLUENTS
...uired. For this purpose, samples
were taken from the effluent, before and after it went through the treatment plant. It was found that the treatment
was not sufficient to remove various physical and chemical parameters including Suspended solids (SS), Biological
Oxygen Demand (BOD), Chemical Oxygen Demand (COD) and Sulfide (S) in order to comply with Pakistan National
Environmental Quality Standards (Pak-NEQS).Th...

 Dr. Taimur Usman*, Dr. Rashid Rehan*, Dr. Salman Saeed*

QUANTIFYING THE EFFECTS OF UPASS: A TRANSIT DEMAND MANAGEMENT STRATEGY
...ne such measure is Universal bus Pass (UPass) which has been proven to be an effective solution for
transit demand management. While focusing on educational institutes across North America, this research quantitatively
analyzes the effects of UPass on transit ridership for the first time. In general, an increase of 50 % has been
observed in transit ridership due to UPass implementation and all the cities where UPass was impl...

Muhammad Usman Shafi*, Syed Muhammad Ali**, Syed Akhtar Ali Shah***, Kamran Ahmed****

DEVELOPMENT OF DATABASE OF HEAVY TRUCK LOAD DATA IN PESHAWAR, PAKISTAN
...ad applied through Universal
testing machine (UTM). Load verses strain graph was developed for each load cell which was used to interpret the
value of load exerted by each pair of tire to produce strains. By adding the strains from each plate, axle load and
similarly by adding axles, the load of the complete vehicle can be determined. Field calibration was carried out to
check the precision of plates and was foun...

 Asif Ali Khan*, Nasru Minallah*, Shahbaz Khan**

ON THE PERFORMANCE OF SUPERVISED CLASSIFIERS FOR CROP IDENTIFICATION AND ESTIMATION USING MULTI-SPECTRAL IMAGERY
... estimation using SPOT-5 satellite imagery. We specifically considered
tobacco as our pilot crop and compared the obtained results with manually delineated calculations. For
this research SPOT-5 imagery of 2.5m spatial resolution, was provided by Space and Upper Atmosphere Research
Commission (SUPARCO), space agency of Pakistan. After preprocessing, which is a preparatory step in analyzing
and classifying

 Gulzar Ahmad1*, M. Inayatullah Babar1, M.Irfan1

A NOVEL WIDEBAND MICROSTRIP PATCH ANTENNA FOR SATELLITE COMMUNICATIONS IN KU-BAND
...na has been proposed for satellite communications.

The bandwidth has been increased using parasitic patches. The size of the proposed antenna is 15x8mm2. It has
been observed that the antenna has a bandwidth of 4.08GHz, a return loss of 49.07dB at the center frequency, a
maximum gain of 8.25dBi and total efficiency of more than 90%. This antenna can be prospective candidate for
sa

 Mahmood Alam Khan1*, Afzal Munir1, Muhammad Shahzad Khattak1, Daulat Khan1, Muhammad Ajmal1

GROUNDWATER RECHARGE QUANTIFICATION FROM RAINFALL IN PESHAWAR DISTRICT-KHYBER PAKHTUNKHWA
...
current pattern of usage are threats to human welfare, livelihood, development, and for the life itself, in the coming
years. The main objective of this study was to quantify the ground water recharge due to rainfall on yearly basis of
Peshawar district Khyber Pakhtunkhwa Province of Pakistan. Groundwater recharge was estimated by subtracting
runoff from the rainfall. The Soil Conservation Service Curve Number (SCS-CN) ...

 Sajjad Hussain1*, Nisar Mohammad1, Zahid Ur Rehman1, Noor Mohammad1, Ishaq Ahmad1,

Naseer Muhammad Khan2 and Salim Raza1
EXTRACTION OF GOLD AND SILVER BY AGITATION CYANIDATION PROCESS FROM COMPLEX ORE OF SHOUGHUR AREA CHITRAL, KYBER PAKHTUNKHWA PAKISTAN
...g process is the most versatile processing technique used for extraction of gold utilizing

different cyanidation parameters likes grinding time, pH, cyanidation time, sodium cyanide (NaCN) and lime CaO..
The efficiency of cyanidation process depends on dosage of each parameter. In present research work the extraction
of gold and silver by cyanidation process from shoughur area of district Ch...

Nisar Mohammad*, Mohammad Mansoor Khan* Noor Mohammad*

CHARACTERIZATION OF TALC-CARBONATE ROCK OF MINGORA EMERALD MINES, DISTRICT SWAT, KHYBER PAKHTUNKHWA, PAKISTAN
...essing technique for the said talc carbonate rocks. Thorough investigations were conducted using SEM-EDX X-Rays Diffraction (XRD) , Thermal Gravimetric Analysis&nb...

 Khizar Azam*, Abdul Shakoor*~ Afzal Khan*, Riaz Akbar Shah*~ Shaukat Ali Shah·

IDENTIFICATION OF VITAL VEHICLE MECHANICAL DEFECTS CAUSING ROAD TRAFFIC ACCIDENTS IN PAKISTAN
..., based on a convenience sample of 290 vehicles was also done at the Toll plaza of the Peshawar-Islamabad Motorway (M-1). The response rate to the survey was 64%. ...

Tabassum Yasmin*, Muhammad Sadiq*

IMPACT OF LANTHANUM DOPING ON SAC305 LEAD FREE SOLDERS FOR HIGH TEMPERATURE APPLICATIONS

 Fcroz Shah*~ Mohammad A. Irfan**, Rizwan Gul*~ Abdul Shakoor*

ALGORITHM DEVELOPMENT AND IMPLEMENTATION FOR NUMERICAL DETECTION OF HOT SPOTS IN CASTING GEOMETRY
...sian: normal;">engine saddle with and without holes were located. This technique shows good agreement to location of shrinkage cavity or porosity observed in cast

 Muhammad Khurram Ali*, Raft Javed Qureshi**, Mirza Jahanzaib***

SELECTING FACILITY LOCATION USING HYBRID METHODOLOGIES IN GLOBAL PERSPECTIVE: A CASE OF CEMENT PLANT
...l;">compatible to the said problem. This paper addresses a real world case study of the problem of selecting a country best suitable for a new cement plant installation among the list offour alternatives. The irreversible nature of the crucial decis...

 Abdul Malik*, Abdul Sattar Shakir**, Muhammad Jamal Khan***, Muhammad Latif**, Zia-ul-Haq*

YIELD RESPONSE AND WATER USE EFFICIENCY OF SUGAR BEET IN SEMI-ARID ENVIRONMENT UNDER DIFFERENT DEFICIT IRRIGATION REGIMES
...nd 9.10%, however it may save 20% irrigation water that can be used to bring more area under irrigation.

...

Nisar Mohammad٭, Mohammad Mansoor Khan٭, Noor Mohammad٭

EXTRACTION OF HIGH QUALITY TALC FROM TALC-CARBONATE ROCK OF MINGORA EMERALD MINES BY FLOTATION AND LEACHING
...a metaphosphate as depressant for carbonates, equal mixture of oleic acid and kerosene oil as collector gave encouraging results. It was found that talc can be separated as float at pH 6 - 7±0.2 in rougher flotation and 10 - 11±0.2 in cleaning flotation, depressant dosage 0.10kg/ton, and collector dosage of 1.2 kg/ton. Talc concentrate at r...

Saeed Gul*, Muhammad Irfan*, Hazrat Bilal*, Atta-ur-rehman*, Umair Khan*, Sadaqat ullah khan*

RECYCLING OF USED ENGINE OIL USING SOLVENT EXTRACTION AND DISTILLATION
...was also obtained at the same process conditions.
...
Khizar Azam٭, Abdul Shakoor٭, Riaz Akbar Shah٭, Afzal Khan٭, Shaukat Ali Shah٭,
Muhammad Shahid Khalil٭٭
COMPARISON OF FATIGUE RELATED ROAD TRAFFIC CRASHES ON THE NATIONAL HIGHWAYS AND MOTORWAYS IN PAKISTAN

Farid Ullah Khan*

ACTUATION ENHANCEMENT OF A MICRO BIMORPH THERMAL ACTUATOR
...e also fabricated by the same generic fabrication process in order to show the practical utility of this research in the field of MEMS. The DMDs actuated by BTAs, are easy to fabricate and can scan through larger angles as compared to electrostatically actuated DMDs.
...

Muhammad Waseem*, Irshad Ahmad**, Rawid Khan**, Bashir Alam**, Khan Shahzada**, Muhammad Javed**

STABILITY ANALYSIS OF SOIL SLOPE IN SWABI, PAKISTAN
... both dry and completely saturated conditions. Pseudo-static approach was used for the seismic stability analysis. The peak ground acceleration value for stability analysis was obtained from the seismic hazard analysis. Two soil slope conditions were considered, and one was found in critical condition. Retaining wall, Soil nails and ground anchors were assumed in the analysis for stability of slope with critical condition. Peak ground acceleration value of 0.2...

Syed Farasat Ali Shah*, Amjad Naseer**, Rashid Rehan**

USE OF RUBBER WASTE IN CONCRETE TO REDUCE ENVIRONMENTAL POLLUTION- AN OVERVIEW
...e. Therefore, it is necessary to reuse these rubber wastes to reduce environmental pollution. Rubber wastes can be used in concrete as a partial replacement of aggregates resulting in lightweight concrete. Using rubber wastes in concrete enhances the impact resistance, thermal insulation and sound insulation at affordable strength. This paper reviews the viable use of rubber wastes in concrete industry to address the problem of disposa...

M. Nafees*, Bushra Khan*, Robina Naz*

STUDY OF OCCUPATIONAL HEALTH SAFETY IN STEEL RE-ROLLING MILL WITH REFERENCE TO HIGH NOISE LEVEL AND TEMPERATURE
...l at furnace section was satisfactory but that at the re-rolling mill was found to be 102 dB, whereas OSHA has set the limit to be 92 dB for 6 hours work shift. A pair-wise t-test indicated that the noise level at all factory zones was significantly different than that of administration section with a p-value < 0.0001. The re-rolling noise level was found to be extremely significant, followed by furnace and unloading section. Likewise the indoor factory hea...

Azam Khan*, Irshad Ahmad**

TIME HISTORY ANALYSIS OF SEISMICALLY ISOLATED FIXED BASE OFFSHORE PLATFORM

Azam Khan*, Irshad Ahmad**

IN-PLACE ANALYSIS OF JACKET OFFSHORE STRUCTURE UNDER STORM CONDITIONS
Muhammad Qasim*, Mohib Ahmad*, Mohammad Inayatullah Babar*, Shahid Bashir*,
Farah Mahmood*, Zeeshan Sabir*
INVESTIGATING THE EFFECTS OF VARIOUS PARAMETERS ON EFFICIENCY OF PHYSICAL LAYER DEPLOYED FOR WIRELESS SENSOR NETWORK
...ortant in the context of saving energy of nodes involved in wireless sensor network communication. Poor quality of communication puts constraint on the already existing energy sensitive nature of wireless sensor networks. This research work is focused on investigation of critical parameters and their effects on overall communication quality in reference to Physical Layer. One of the key factors is antenna design and other factors include ph...
Naeem Ejaz*, Daulat Khan**, Usman Ali Naeem*, Muhammad Ali Shamim*, Muhammad Fiaz Tahir*, Faisal Shabbir*, Jawad Hussain*
DETERIORATION OF ORDINARY PORTLAND CEMENT AND FLY-ASH BASED GEO-POLYMER CONCRETES DUE TO SULFATE ATTACK UNDER THE ACTION OF SULFURIC ACID AND WASTEWATER
...sp;has not been resolved satisfactorily yet. Generally, deterioration happens due to sulfuric acid reaction with treatment units and sewer materials. Geo-polymer binders especially fly ash (FA) is an acid resistant and can be used as a substitute binder for sewer construction. This research work highlights the laboratory results of fly ash based geo-polymer concrete and effects on its durability under sulfuric acid exposure. Class ‘F&rsquo...

Muhammad Irfan*, Saeed Gul*, Mohammad Younas*

SOLAR STILL PERFORMANCE FOR SEA WATER PURIFICATION: A STATISTICAL AND EXPERIMENTAL STUDY
... with 240 mg/L dissolved salt has been extracted from the model sea water having dissolved salts of 35000 mg/L. The efficiency of a single basin solar still is improved up to 80 percent. 
...

Mumtaz Shah*, Hashim Nisar Hashmi* Naeem Ejaz* Abdul Razzaq Ghumman*

PERFORMANCE EVALUATION OF AQUATIC MACROPHYTES FOR MUNICIPAL WASTEWATER TREATMENT
...uent and effluent sewage samples. During the entire study, the average reduction of 51% for BOD5, 47% for COD, 19% for phosphorus, and 41% for nitrogen were found with water hyacinth. When the same sewage was treated with Duckweed the values came out to be 34% for BOD5, 27% for COD, 16% for phosphorus and 18% for nitrogen. Similar trend was observed in the experiments with Water Lettuce as 33% for BOD5, 2...

Asif Nawaz*, Muhammad Zafarullah Khan, Rehmat Ullah, Khalid Nawab and Urooba Pervaiz

Investigation of Professional Competency Level and Training Needs of Field Assistants in Khyber Pakhtunkhwa
...nt Khyber Pakhtunkhwa. A sample of 234 respondents was selected and personally interviewed through pre-tested and validated interview schedule. Paired sample t-test and dummy variable regression model were used to achieve study objectives. It was found that Field Assistants were highly competent in conducting farm/home visits followed by conducting meetings and organizing extension campaigns. Highest training needs in profes...

Fazli Rabbi1*, Muhammad Idrees2, Shahid Ali1, Muhammad Zamin3 and Hazrat Bilal4

Farmers Perceptions and Adoption of Information and Communication Technologies (ICTs) in Peach (Prunus persica L.) Production and Marketing
... farm level variables. A sample of 70 peach (P. persica L.) growers was taken. Face to face interviews were held using a well-structured questionnaire. Descriptive statistics and χ2 tests were used for data analysis. The results showed a greater awareness level among the peach (P. persica L.) growers about ICTs. Moreover, the analysis indicated that more than 75 % of farmers are in use of a single or multiple type of ICTs. Among the <...

Sajad Ali1*, Jangraiz Khan2, Arshadullah Jadoon3, Muhammad Riaz4 and Abid Khan1

Evaluation of Farmers Socioeconomic Characteristics Influencing Tomato Output in District Peshawar, Khyber Pakhtunkhwa, Pakistan
...t is becoming an indispensable source of farmer’s income in Pakistan. The production of tomato has been decreased considerably during the last five years. The present study has been conducted to explore the farmer’s socio-economic characteristics, affecting the tomato production in district Peshawar, Khyber Pakhtunkhwa, Pakistan. A multi-stage-sampling technique and interview method were adopted for data collecti...

Syed Mufeed Hadi Naqvi1*, Badar Naseem Siddiqui1, Waqar-Ul-Hassan Tareen1, Muhammad Ameer Qarib Naqvi2 and Naseeb Hussain3

Usage of Mobile Phone by Vegetable Growers and its Impact on Vegetable Production
...e factors affecting the usage of mobile phone in vegetable production. Out of four, two tehsils from district were selected by using simple random sampling, then four union councils from each tehsil and three villages from each union council, also selected at random. Due to the limitations of study such as time and financial support five farmers from every village were selected to make a total one hundred and twenty responde...
Sitara Nawaz1, Shamaila Irum1, Haroon Ahmed2, Shazia Shamas1, Sadia Roshan1, Muhammad Ather Rafi4, Irfan Ullah5*, Gulnaz Parveen3*
...Pro";">Bloodsucking sandflies belonging to dipteran are vectors of human disease called leishmaniasis. It is one of most neglected diseases in our country. The main purpose of the study was to identify the prevalence of sandflies in selected regions of Panjab and their relationship with hospital-reported cases of Leishmaniasis. Clinical information of 124 patients was recorded. Observable factors of prevalence include a...
Sumaira, Ali Mujtaba Shah*, Ghulam Asghar Solangi, Ifra Anwar, Qudratullah Kalwar 
...ctions. Thus, it is necessary to illuminate the beneficial impact of camel milk and its composition. 
 
Zarish Yaqoob1, Saleema Bashir Shams2, Gaitee Joshua2 and Bibi Nazia Murtaza2,3*
...es to the human health disasters. River is a trans-country river originating in Himachal Pradesh, India and joins Indus and its tributary rivers in the Punjab province of Pakistan. The present study describes the concentration of heavy metals in the water and different tissues of native fish collected from three different areas. The highest concentration of Cr (1.451 µg L-1) and Co (0.325 µg L-1) was measured in the river water at Ballok...

Muhammad Riaz1*, Naureen Akhtar1, Salik Nawaz Khan1, Muhammad Shakeel1,2 and Ateeq Tahir1

Neocosmospora rubicola: An Unrecorded Pathogen from Pakistan Causing Potato Stem Rot
...ar the collar region. Causal organism was isolated from the infected tissues, purified and identified on the basis of morphological characters, nucleotide sequences of internal transcribed spacer region (ITS) and partial beta tubulin gene. Phylogenetic analysis was also conducted to determine the phylogenetic relationship of this species with other reported species of this genus. Pathogenic potential of the isolate was verified by artificially inoculating the ...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...th climate change at the same time. It is a fact that sustainable development comes only by judicious utilization of the resources and environment protection that help in addressing socio-economic challenges. An integrated farming system (IFS) holds a special position with the central concept that nothing is supposed to be wasted here. It is an integrated approach to farming compared with existing monoculture approach. It passes on to an agricultural system th...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...th climate change at the same time. It is a fact that sustainable development comes only by judicious utilization of the resources and environment protection that help in addressing socio-economic challenges. An integrated farming system (IFS) holds a special position with the central concept that nothing is supposed to be wasted here. It is an integrated approach to farming compared with existing monoculture approach. It passes on to an agricultural system th...

Mohammad Aslam Khan*, Samiullah* and Zulfiqar Ali*

DYNAMICS OF URBAN SPATIAL GROWTH AND PLANNING: A CASE STUDY OF PESHAWAR
...oric maps, documents and satellite images. The analysis shows that the city has been growing at a rapid pace adding about 500 ha of land annually in recent years and in the absence of planning control has seen tremendous urban sprawl. The pattern of development of urban sprawl in Peshawar is typical of most Pakistani major cities, where ribbon sprawl is common along major roads, while leapfrog sprawl is prevalent in the city’s fringes...

Shahida Nasreen Zakir*, Samina Siddiqui*, Nasreen Ghaffar* and Zia Ul Haq**

WATER QUALITY INDEX OF SHALLOW AND DEEP GROUNDWATER DURING WET AND DRY SEASON OF PESHAWARBASIN
...l of 95 groundwater samples at 1.5 to 30 m depths were collected in triplicate during wet and dry seasons. All samples were analyzed for pH, EC, TDS, SO4 2-, HCO3 -1, CO3 -2,Cl-1, Ca2+, Mg2+, Na+ , Fe+3 and K+ The result of this study showed that according to water quality index (WQI) of WHO all groundwater samples were categorized as good water and considered suitable for d...

Asadullah Saleem* and Nadeem Ehsan*

STATUS OF PERFORMANCE MEASUREMENT SYSTEMS IN INFRASTRUCTURE CONSTRUCTION FIRMS OF PAKISTAN
...performance drivers necessary for achieving long term goals.
...
Naeem Ejaz*, Daulat Khan**, Usman Ali Naeem*, Muhammad Ali Shamim*, Usman Ghani* and Muhammad Fiaz Tahir*
INVESTIGATION TO CHECK THE FEASIBILITY OF COMPOSTING FOR SMALL CITIES IN DEVELOPING WORLD: A CASE STUDY OF TAXILA CITY
... thirty and fifteen sampling sites are selected from each of these zones respectively. Solid waste samples are collected from the selected sites for a week on daily basis, which are then analyzed to find their composition and are recorded accordingly for each zone. The analyses of collected samples indicated that almost 60-70% of generated communal waste from all three ...

Sajid Ullah Butt, Aamer Ahmed Baqai, Syeda Naela Fatima, Hasan Aftab Saeed

MATERIAL HANDLING COST CONSIDERATION IN PROCESS PLAN SELECTION FOR RECONFIGURABLE MANUFACTURING SYSTEM
...ly included are machine usage, tool usage, machine change, tool change costs etc. All the parameters related to these costs become a part of the process plans. The focus of this research is to include material handling equipment such as transport and positioning equipment in the process plans and hence, to include their usage cost in the overall mathematical model. This will lead to a more...
Faheem Ali1, Muhammad Amir1, Muhammad Iftikhar Khan1, Muhammad Naeem Arbab1, Abdul Basit2, Muhammad Kashif Khan1, Gulzar Ahmad1
AN SVC BASED POWER QUALITY IMPROVEMENT MODEL FOR CONSUMERS CONNECTED TO WEAK BUSSES IN DISTRIBUTION SYSTEMS
... a Static VAR Compensator (SVC). An SVC based Power Quality Improvement (PQI) is proposed in this paper which addresses the real time varying reactive VAR requirement with reduced harmonic content.
...

Mehreen Ijaz

COMPARATIVE ANALYSIS OF TENSILE STRENGTH OF MULTIFILAMENT SILK AND VICRYL SUTURES USED IN SURGERY
Amjad Hussain1, Ata Ur Rehman2, Zia Ur Rehman Tahir3, Aftab Ahmad1, Fahad Noor3, Qasar Wasique Ahmad1
INVESTIGATION OF EMPLOYEES’ PERCEPTION ABOUT LEAN IMPLEMENTATION
...are likely to be more dissatisfied with their jobs. The study also identified the factors which contribute towards the development of negative perceptions. However, the study is limited to one lean tool (Kaizen) and its implementation in textile sector. The results of the study provide opportunity to understand perceptions about lean implementation and their relationship with job satisfaction and how perceptions can be ...

Behram Khan1, Amir Nawaz Khan2, Said Qasim3, Atta-ur Rahman4

ANALYSIS OF HUMAN PERCEPTIONS AND ADAPTATIONS TO FLASH FLOODS IN KHYBER AGENCY, FATA
... adult residents in five sample villages along Chora River and its tributaries were interviewed by using a structured questionnaire which covered approximately 20% of the total households. Respondents’ perceptions were investigated about the occurrence of flash floods, flood damages, nature of affinity towards flood prone areas and adaptation strategies. Cartographic and statistical techniques were applied to process and analyze the data. As a result, th...

 Gulzar Ahmad1, M. Inayatullah Babar2

AN EFFICIENT PATCH ANTENNA WITH IMPROVED BANDWIDTH FOR FUTURE APPLICATIONS
...ze has been proposed for satellite and other fields of communications in future. The size of the antenna is very small and will definitely require a small space in the electronic equipment. An efficient substrate material was employed in the antenna that is known as Preperm L-450. Dielectric constant of this mentioned material is 4.4 and its height or thickness is 1.64mm. Different methods were engaged to expand its bandwidth from 9.07GHz t...

 Amir Naveed1, Unsia Habib1, Fouzia Parveen2, Syed Naveed Ul Hasan1, Muddasar Habib1, Irfan Shah1

AN INVESTIGATION OF HEAVY AND TRACE ELEMENTS IN COAL DEPOSITS OF MAKARWAL PAKISTAN AND THEIR POSSIBLE IMPACTS ON SURROUNDING WATER-CASE STUDY
...search present, the coal samples from different locations of Makarwal (Pakistan) were investigated for the existence of trace and heavy metals. Coal samples used under investigation were: Makarwal section-A (Mk-A), Makarwal section-B (Mk-B), Kurd section C (Kr-c), Makarwal section D (Mk-D) and Gula-khel section E (Gk-E). It was observed that, these coalfields contain arsenic (As) ranging from 3.0–16.34mg/kg, ...

 Saman Shahid1, Shahid Ali1, Muhammad Abdullah Ahmad1, Sarfraz Munir2

RE-DESIGNING AND OPERATIONAL ANALYSES OF THE SEDIMENT TRANSPORT CONTROL IN IRRIGATION CANAL NETWORK
...imized which can help in saving billions of tax payer money spent on “Bhal Safai” and maintenance. 

...

 Nashitah Alwaz1, Safdar Raza1*, Sadaqat Ali1

A COMMUNICATION-FREE SIGNAL GENERATING TECHNIQUE FOR REACTIVE POWER CORRECTION IN ISLANDED MICROGRID
...hnique has the following salient features: Accurately shares the reactive power without the information of feeder line impedances/ This accurate reactive power sharing scheme has been started locally without the need of communication signal from the central controller. Thus, there is no delay in monitoring the notable changes in microgrid caused by DGs. The careful selection of PI & droop controller parameters through PSO makes the syst...

 Shakil Rehman Sheikh1*, Zafar Ullah Koreshi1, Nouman Ghafoor1, Muhammad Shahid Khalil1, Muzzamil Ghaffar1

MITIGATING CO2 EMISSIONS OF ACADEMIC BUILDINGS IN PAKISTAN USING ENERGY CONSERVATION TECHNIQUES
...">Energy use data of the sample school was gathered from available records as well as measured using an in-house developed metering device that recorded the electric consumption data on hourly bases. Building energy simulations were also carried out using TRNSYS© software for validating and comparing the actual energy consumed and optimal energy consumption resulting from building energy simulation of the existing and re-designed building. The
...

Shakir Azim1*, Sahar Noor1, Rehman Akhtar1, Qazi Salman Khalid1, Tufail Habib1, Usman Ghani2

SETTING OF PROCESS PARAMETERS AND EFFECT OF MOULD QUALITY ON SURFACE ROUGHNESS OF PHARMACEUTICAL CUP OF POLYPROPYLENE
...neering articles to disposable consumer products. As of late, the production of plastic items has expanded quickly in light of the fact that plastic items are low in price, light in weight and quick for shape framing, along these lines making them more in vogue than the metallic products. As a result of mounting plastics applications, growing customer increased, the quality prerequisites of injection molded items have turned out to be progressiv...

 Riaz Sayed1, Khizar Azam1, Waqar Shah1, Inayat Babar1, Hamidullah1, and Sahar Noor1

A REVIEW OF DRIVING UNDER THE INFLUENCE OF FATIGUE
...e. Around the world, thousands of lives are lost each day due to road accidents. The National Road Safety Secretariat (NRSS) Pakistan estimates, the annual economic cost of highway crashes over rupees one hundred billion (over US$ 1.6 billion) for Pakistan. This paper provides a comprehensive review of the previous literature regarding driver fatigue and drowsiness research, the present state of research and the future trends. Various facto...

Amjad Ali1, Hong Luo1, Khalid Saifullah Syed2, Muhammad Ishaq2

A PARALLEL DISCONTINUOUS GALERKIN CODE FOR COMPRESSIBLE FLUID FLOWS ON UNSTRUCTURED GRIDS
...iple Data (SPMD) message passing parallel programming model has been employed, by making use of the de-facto industry standard Message Passing Interface (MPI) library. Favorable parallelization characteristics of the discontinuous Galerkin scheme have been exploited for hiding the communications behind the computations. The parallel performance of the developed code on an Ethernet based cluster, in terms ...

 Laiq Khan1, Sidra Mumtaz2, Kamran Javed3, S.W. Shah4, M.I. Khan Babar5, S.R. Akbar6

GENERATORS MAINTENANCE SCHEDULING USING HARMONY SEARCH ALGORITHM
...;concept of music improvisation process of searching for a perfect state of harmony. To verify the great power of this method, HS algorithm is applied to solve GMS problem of different power production systems. Simulation results reveal that the proposed algorithm is a powerful search algorithm for various optimization problems.
...

 Muhammad Naeem Khan Khattak1, Hamidullah1, Shehryar2

PROBLEMS, ANALYSIS AND RECOMMENDATIONS OF THE AUTOMOTIVE VENDOR INDUSTRY OF PAKISTAN—I
...ndustry of Pakistan. The salient outcomes of the analysis are presented. On the basis of the analysis and interview results, comprehensive recommendations are also provided for the automotive vendor industry of Pakistan.
...

 Usman Ali Naeem*, Hashim Nisar*, Habib-ur-Rehman** and Naeem Ejaz*

DEVELOPMENT OF FLOOD DISCHARGE MODELS USING GIS
...s River is considered as salvation of Pakistan and it is also the largest river of the country. The approximate length of the river is 3180 kilometers and its drainage area exceeds 1165000 km2. Having in view the worth of Indus River and its socio economic implication on Pakistan it was planned to carry out research work on upstream of Terbela from Kachura. The intended approaches of this work were to generate empirical equations based on h...

Arshad Habib Malik*, Aftab Ahmed Memon** and Muhammad Rafiq Khan***

DYNAMIC MODELING AND CONTROLLER DESIGN FOR NUCLEAR POWER PLANT
...and discrete fast output sampling control synthesis for PHWR (Pressurized Heavy Water Reactor)-type nuclear power plant is presented in this paper. The nonlinear dynamic model of PHWR power reactor is developed based on reactor, logarithmic amplifier, moderator level control valve and reactivity system. The nonlinear model is characterized by 15 state variables and one input. This higher order nonlinear model is linearized at full power ope...

Rafi Javed Qureshi* and Iftikhar Hussain**

INTERNATIONALIZING MANUFACTURING SMEs IN A DEVELOPING REGION: AN EXHAUSTIVE CDA-BASED EXPLORATORY APPROACH
...austive approach is necessary to explore the adequacy of factors in question. The three methods relevant to categorical data analysis (CDA) in this paper highlights the enabling role of the exploratory factors in understanding the relationship with SME’s exporting. Categorical regression overcomes the limitations of both contingency table method and binary logistic regression in exploring this relationship, and provides substantive sp...

 Muhammad Waseem*, Irshad Ahmad**, Muhammad Asif Khan**

DETERMINISTIC SEISMIC HAZARD ANALYSIS AND SELECTION OF TIME HISTORIES FOR DYNAMIC ANALYSIS FOR A SITE IN SWABI
...about a hundred thousand people and monitory losses of about five billion US dollars. After this earthquake, most of research activities were directed to define earthquake hazard. However, amongst other problems in those studies, one hard decision is the use of attenuation relationship as no such equation is developed for Pakistan. These studies either do not consider the newly developed New Generation Attenuation (NGA) equations or us...

Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*

Hepatitis E Virus and Zoonosis
...eloped nations with good sanitary conditions where the infection is a zoonosis. Though the virus has only one serotype, 8 genotypes have been documented. Out of these, HEV 1 and 2 have been reported from developing countries and are transmitted mainly through faeco-oral route while HEV 3 and 4 are reported from developed countries and the infection is acquired by humans due to the eating of undercooked pigs, deer and wild boar meat. Recently HEV genotypes 5 an...

Javed Iqbal1, Ali Zohaib1*, Muzzammil Hussain1, Iftikhar Ahmad1, Adnan Bashir1, Wardah Muzaffer2, Naeem Faisal1, Muhammad Tahir Latif1 and Sami Ullah1

...ties. Wheat varieties (Lasani-08, Faislabad-08, Millat-11, AARI-11 and Punjab-11) were sown on different sowing dates (November 01, November 15, November 30 and December 15). Yield and related traits of all varieties were decreased by delayed sowing. Early sown crop (November 01) had maximum emergence which decreased gradually with delayed sowing. Tillers m-2, grain weight, grains per spike and yield of all varieties was greater on November 15 sowing and decre...

Markus I. Francis1*, Paul I. Ankeli2, Clara N. Kwanashie1, Jibril Adamu1, Lushaikyaa Allam1, Mashood A. Raji3, Godwin O. Egwu4, Flavio Sacchini5 and Massimo Scacchia5

Detection of Mycoplasma bovis from Cattle Presented for Slaughter in Adamawa and Taraba States, Northeastern Nigeria
...hundred and eighty (480) samples of lung tissues (180), nasal swab (180), ear swab (60) and pleural fluid (60) were collected from 190 heads of cattle at slaughter in Yola and Jalingo abattoirs in Adamawa and Taraba States, respectively Samples were processed based on standard laboratory protocols. An overall Mycoplasma bovis isolation rate of 0.83% (4/480) was obtained. Based on the states studied, 1 (0.35%) and 3 (1.53%) M...

Hina Imtiaz1*, Salma Khalid2, Kashaf Riaz1, Muhammad Arshad Ullah3 and Zubair Rehman

...Nullah Lai stream. Water samples were collected from different points of the stream and tested for basic parameters like color, odor and turbidity while EC, pH and TDS were checked on-site by portable water quality meter. The assessment of heavy metal concentrations i.e. Lead (Pb), Copper (Cu), Nickel (Ni), Chromium (Cr), Zinc (Zn), Mercury (Mg) and Cadmium (Cd) were carried by Atomic Absorption Spectrophotometer. Significant concentrations of heavy metals wer...
Sinan Uzundumlu1, Yesim Buyukates1,*, Murat Yigit2, Musa Bulut2,  Rıdvan Kaan Gurses1 and Barbaros Celikkol3
...ers such as temperature, salinity, pH and dissolved oxygen, total suspended solids, chlorophyll-a and inorganic nutrients such as NO2+NO3, NH4, PO4 and SiO2 were observed between May 2014 and September 2014 in the study area. The observed results were compared with acceptable limits pronounced in international organizations such as EPA and FAO, and national organizations such as WPCR and RTMAF as well as the previous...
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
... small;">Skeletal muscle satellite cells (SMSC) are a population of multipotent cells that exist not only during animal infancy, but are also present in the adult mammalian body. Previous studies have indicated that Wnt/β-catenin signaling is involved in the regulation of multipotent cell differentiation and is essential for the regulation of adipogenesis. However, the differentially-expressed microRNAs (miRNAs) involved in SMSC adipogenesis induction, an...

Muhammad Mazhar Iqbal1*, Ijaz Ahmad2, Shahzad Maqsood Ahmed Basra3, Abu Baker Ijaz4, Zahid Hassan Tarar5, Muhammad Ansar6, Umer Iqbal7, Tayyaba Naz4, Bilquees Fatima8, Muhammad Akram9, Allah Wasaya10, Asif Iqbal11, Shakeel Ahmed Anwar12, Khalid Saif Ullah Khan13, Azeem Khalid14, Asif Aziz15 and Rashid Mehmood16 

...ydrogen peroxide (H2O2), salicylic acid (SA) and ascorbic acid (AsA) may induce heat stress tolerance. Spray of SA, AsA, and H2O2 increased chlorophyll, relative water and nutrient contents, membrane stability index (MSI) and antioxidants activities in heat stress. Moreover, foliar application of chemicals during norma...

Ghulam Murtaza1, Ghulam Sarwar1*, Muhammad Ashraf Malik2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1 and Sher Muhammad3 

...ustify;">Irrigation with saline water is a key issue affecting crop growth. Saline irrigation has drastic effects, limiting normal physiological activity and productive capacity of crops. The saline water irrigation leads to salt accumulation in the vicinity of roots which results in reduced yield along with soil deterioration. Organic matter application can prove helpful in keeping the

Zahid Hassan Tarar1, Muhammad Salik Ali Khan2*, Shahzada Munawar Mehdi3, Raza Salim4, Irfan Ahmad Saleem1, Saima Nazar5, Munir David2, Tahir Majeed6, Muhammad Sufyan Mughal7, Muhammad Saleem8, Umer Iqbal9, Muhammad Mazhar Iqbal10 and Muhammad Khalid Shaheen

... 1194 georeferenced soil samples were collected at a depth of 0-15cm maintaining a sampling grid cell size of 10-acres (40469 m2) throughout the sampling area. These samples were analyzed for plant available Zinc (Zn), Copper (Cu), Iron (Fe), Manganese (Mn) following Diethylenetriamine Penta-Acetate (DTPA) extraction methodology. Quantification was carri...

Zakeer Ahmed Khan Abbasi* and Allah Nawaz 

...e responses are not necessarily identical in scale and forms . In developed countries, climate change awareness is much advanced therefore, their climate change adaptations are also far better than those employed by farmers in the developing countries like Pakistan. The climate change awareness obviously determines the nature, quality and strength of climate change adaptations. The current study measures the impact of climate change awareness on climate change...

Matiullah Khan1*, Humera Arif2, Muhammad Rasheed2, Motsim Billah3 and Shoaib Ahmad

...akistan. Out of 1279 thousand nutrient tons consumption of P fertilizers in the country during the year 2017-18, 85.4% was imported. The most expansive P fertilizers’ application is continuously increasing the cost of crop production. The huge amount rock phosphate (RP) found in Hazara area of Pakistan can be utilized as P source by preparing Single Super Phosphate (SSP). The spatial variation in minerology of Hazara RP might have an impact on the availa...

Haiyan Yang1, Hongxia Liu1, Wenlong Wu1*, Weilin Li2 and Lianfei Lyu

Roni Koneri1*, Meis Nangoy2 and Pience V. Maabuat1
...f each habitat type, and sampling was carried out along the lines using sweep nets. From the research, 7 families, 20 species and 1,750 individuals belonging to 2 suborders, Anisoptera and Zygoptera, were identified. Libellulidae was the family with the most number of species and individuals being found. Th especies with the highest abundance was Orthetrum pruinosum, followed by Libellago xanthocyana. The highest dragonfly species abundance was f...
Faruk Çolak1 and Ferhat Matur2,3,*
... conspecifics within the same cytotype or species.
...
Magbolah Salem Helal Alzahrani
...olatile oils including bisabolol, bisabolol oxides A and B, and matricin as well as flavonoids. This study aims to elucidate the possible therapeutic applications of different levels of M. chamomilla on the functional status of liver, in rats injected with carbon tetrachloride (CCl4), a known hepatotoxin. Thirty-six male albino rats were treated with subcutaneous injection of CCl4 in paraffin oil...

Olga Mikhailovna Blinnikova1*, Vadim Anatolyevich Babushkin1, Lyudmila Gennadievna Eliseeva2 and Galina Severyanovna Usova3

Modeling a Formulation and Assessment of the Consumer Properties of the Special Purpose Starch Drink
...ly active agents. At the same time the new types of drinking kissel useful to health, in assortment are which confirms the feasibility of updating the range due to enriched drinking kissels. Drinking of one glass of kissel covers the body daily need for ascorbic acid for 211.5%, P-active agents for 226.9%, including catechins for 82.6%, and for 200% in collagen. The benefit of the drink is due to simultaneous presence in the kissel of a high dose of data vitam...

Syeda Farzana Bibi* and Siraj-ud-Din

Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay
...y. Brine shrimp larvae assay was conducted for this purpose. Extractive values of leaf and bark parts were estimated using n-hexane, chloroform, ethanol, methanol, water, and ether as a solvent. 10g of plant powder was dissolved in 200 ml solvent during various extract preparations Fluorescence study was conducted after treating the powder drug with different reagents including ethanol, methanol, acetic acid, n-hexane, HCl, nitric acid, car...

Kamran Ghasemi1*, Mostafa Emadi2, Asghar Bagheri3 and Mehdi Mohammadi1

Casing Material and Thickness Effects on the Yield and Nutrient Concentration of Agaricus bisporus
... (4.43 Kg m-2). The same results were obtained in total yield of compost and biological efficiency. The CCS treatment induced the first flush to be in advance, however, the CP procrastinated the first flush. Overall, the highest average weight of mushroom and the mushroom cap diameter were observed in 4 cm depth casing. No significant deference was observed in the phosphorus and potassium concentration of treated mushrooms but nitrogen conc...
Waqar Majeed1, Naureen Rana1, Elmo Borges de Azevedo Koch2* and Shahla Nargis1
...cking, forceps. For each sampling day in each month, 20 sweeps were randomly taken in each block. The insects at rest or on shrubs were manually collected. Overall, 5867 individuals were recorded pertaining to 152 species. Among the arthropods collected from two fields, number of the order Diptera was most diverse followed by those of Coleoptera, Hymenoptera, Hemiptera, Orthoptera, Lepidoptera, Araneae and Neuroptera. The number of arthropod species differed b...

Mazhar Abbas1*, Kishwar Jam1, Rashid Iqbal Khan1, Muhammad Zafar-ul-Hye2, Tariq Rafique3 and Zahid Mahmood

...rients as well as acetyl-salicylic acid combined with ascorbic acid can be used carefully for expecting efficient productivity with high quality produce. 

...
Mahak Fatima1, Memoona Syed1, Rabia Zeeshan2, Farah Rauf Shakoori3, Naveed Shahzad4, Moazzam Ali1 and Zeeshan Mutahir1*
...ing and cell viability assay. As a result of siRNA transfection, MTH1 protein was knockdown to approximately 77% in the transfected sample and resulted in a 1.75-fold increase in sensitivity of MCF7-R cells to gemcitabine. Moreover, higher expression of p21 protein was also observed in transfected MCF7-R cells that may indicate induced cell death. This study highlights the effect of MTH1 gene silencing in drug-resista...
Maryam Yousaf1, Naveed Shahzad2, Zeeshan Mutahir1 and Moazzam Ali1*
...inct intracellular localisation for controlling endocytic, exocytic, and recycling pathways. The impairments in Rab pathways have been linked with drug resistance in various cancers. However, the role of Rab21, an endocytic Rab GTPase, in the emergence of drug resistance is not known. Therefore, we aimed to investigate the role of Rab21 in MRP-1, drug efflux pump, mediated resistance in prostate cancer cells. Drug sensitive (PC-3/Wt) and epirubicin resistant (...
Hamed A. Ghramh,1, 2, 3,Zubair Ahmad 1, 2, 4, Khalid Ali Khan1, 2, 3*and Farhat Khan4
Muhammad Khan1, Tehmina Ameer Khan1, Aziz Ud Din2, Muhammad Fiaz Khan1, Irfan Ullah3,*, Kalim Ullah4, Sadia Tabassum1,*
...1/500 globally. Eighteen samples from four families of maternally inherited HCM were collected, based on their clinical records. Three mitochondrial encoded genes 16S-rRNA (MT-RNR2), tRNAIle (MT-TI) and tRNALeu (MT-TL1) were targeted for analysis of mutation. Genomic DNA was extracted from buccal swab using Phenol: Chloroform: protocol. The target genes were amplified via polymerase chain reaction (PCR). The amplified gene products...
Zubair Ahmad1, 2,5, Hamed A. Ghramh1, 2, 3,Khalid Ali Khan1, 2, 3*, Kavita Pandey4 and Farhat Khan5
...e importance of apico-dorsal spine of metasoma for taxonomy is discussed. An updated checklist of chelonine leafminer parasitoids from the Indian subcontinent is also provided.
...
Kui Li1, Lijun Li1, Jialun Fu2, Yi He2, Zhaohui Song2, Shuiping Zhou2Luobu Gesang1* and Rui Liu2*
... on day 3 (arrival at Lhasa, altitude 3658 m), AMS symptoms (LLSS=3.79) in the CON group had clearly aggravated (P=0.0475) in contrast to those in the group with administration of CDDPs (LLSS=2.31). Besides, arterial oxygen saturation (SaO2) value for those taking CDDPs was significantly higher than that in the CON group (87.86% vs. 85.72%, P=0.0388) on day 10. These preliminary findings suggested that CDDPs might...
Abdul Waheed*, Asim Faraz, Hafiz Muhammad Ishaq
...stly they kept birds for sale, disliked cock fighting, provided positive gesture about poverty alleviation and showed minimum concern about poultry problems. It is concluded that poultry keeping in rural household provides a potential source of income with minimum health care and problems and adding to the income of households. This small-scale entrepreneurship might bring higher income to rural households and may help in alleviation of poverty a mass scale in...

Ali Hazrat1*, Mohammad Nisar1, Khan Sher2, Jehandar Shah2, Tour Jan1 and Abid Ullah1

Taxonomic and Medicinal Study of Papilionaceae of District Upper Dir, Khyber Pakhtunkhwa, Pakistan
... is fully ripened in the same way as broad beans, fully ripened seed requires overnight soaking to soften it before cooking. All the species of the selected family are reported for the first time and studied for medicinal purposes.

...

Oluwatoyin Olagunju1* and Luqman Abiodun Akinbile2

Farmers Perceptions of the Effect of Rural Transportation Systems on Farming Income in Ondo State, Nigeria
...using a two-stage random sampling technique. Data used for the study were analyzed using descriptive statistics and inferential statistics such as chi-square and Pearson Product Moment Correlation (PPMC). The study revealed that farmers perceived the quality of transportation system causes a reduction in their income. Gender (χ2=6.472) and marital status (χ2=9.745) positively influenced income generated by farmers at p<0.05. Also, there was a signif...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...evealed the formation of same cluster in phylogenetic tree with that of specific subspecies S. g. gregaria which furtherdeviate genetically not only from closely related subspecies (S. g. flaviventris) but also other species reported from various countries. Migratory pattern of S. g. gregaria from Africa to Asia and neighboring countries with their potential routes also suggested developing sustainable policy to counter internal as well as...
He Wenyun, Zhi Yijin and Shao Mingqin*
...e results suggested that safety was the primary factor in habitat selection. The main foraging method was up-ending (61.54%); the duration of each up-ending foraging behavior was 1.92±0.83 s and foraging frequency was 14.67±4.43 times/min. This foraging strategy may be determined by food distribution and morphological structure.
...
Gregory Ejikeme Odo1,*, Juliana Ekenma Agwu1, Nkechi Nweze2, Clara Ikegbunam2,Felicia Ekeh N1, Vincent Ejere1, Ngozi Ezenwaji1, Reginald Njokuocha2, Godwin Ngwu1 and Fabian Okafor1
...ac glycosides, steroids, saponins, and tannins are moderate in abundance (other constituents such as the cardiac glycosides, steroids, saponins, and tannins that were moderate in abundance (++). There was a dose duration dependent (p < 0.05) decrease in RBC in all the groups. There were also duration-dependent decrease in MCV values obtained in this research as against duration and dose dependent increases in MCH, and MCH...

Tao He1,2,3, Cheng-jing Chen4, Jian-guang Qin3, Yun Li1,2, Rong-hua Wu1,2 and Tian-xiang Gao4,*

...his study was to compare sagittal morphometric features between Liza haematocheilus stocks in the China coasts at the Ying Kou, Dong Ying, Qing Dao, Wen Zhou and Guang Zhou regions and test a complementary method to quantify the variation of sagittal shapes to discriminate L. haematocheilus stocks. The sagitta variation of five L. haematocheilus stocks was exami...

 Honghai Zhang1,*, Yao Chen1, Xiaoyang Wu1, Shuai Shang2, Jun Chen2, Jiakuo Yan1, Qinguo Wei1, Xibao Wang1, Yongqiang Lu3 and Huanxin Zhang2

... Park. And we divide the sample into Group A (the low altitude area) and Group B (the high altitude area). we studied the basic structure of the gut microbiota by sequencing high throughput sequencing of the 16S rRNA gene V3-V4 hypervariable regions. The differences of gut microbiota in the Tibetan wild ass at different altitudes were compared. We obtained 1474595 16S rRNA gene sequences. The study observed 163genera belonging to 19 phyla in Group A while 210 ...
Fei Wang1, Xianlai Zhang1, Youbao Zhong2, Xiaoen Tang3, Xiaofen Hu1
ShanShan Yang1, Shengwei Zhong1, Zuohong Zhou1, Jin Liu1, Xu Yuan1 
and Yong Li1*
...ults, the intestinal mucosa epithelium is a single layer of columnar epithelium, other structures are relatively complete, and the intestinal villi and glands are distinct in each part of intestine. The localization of VIP is extensive in the intestinal wall, but most typical in the lamina propria and muscular layer; VIP in mucosal epithelium is mainly distributed within goblet cells. The distribution of VIP is stronger in t...

Muhammad Riaz Gondal1,2*, Aqib Riaz4, Sultan Ahmad Rizvi2, Bushra Zulfiqar2, Waqas Naseem2, Humara Umar3, Ahmad Hussain1 and Mazher Iqbal

...ha-1, Paraquat and Glyphosate @ 1.5 and 2 L ha-1 and Fenoxaprop-p-ethyl (Puma super) @ 1.0 and 1.5 L ha-1 were applied on Pak Sudac variety of S. S. Hybrid and compared their control on weedy grasses with a weedy check (no weedicide application) during summer seasons of years 2018 and 2019. It was observed that application of Glyphosate and Paraquat @ 2 L ha-1, 1.5 L ha-1 reduced the grassy weeds after first cut of S.S. Hybr...

Amar Iqbal Saqib1, Khalil Ahmed1*, Abdul Rasul Naseem1, Ghulam Qadir1, Muhammad Qaisar Nawaz1, Muhammad Khalid2, Imtiaz Ahmad Warraich3 and Muhammad Arif

...teriorated properties of salt-affected soils. For addressing this objective, a study was undertaken to investigate the ameliorative effect of humic acid (HA) with gypsum on rice-wheat crops under saline-sodic condition (ECe = 4.71 dS m-1, SAR = 31.82, pHs = 9.10). Different combinations of gypsum and humic acid tested were: T1 - control, T2 - gypsum @ 10...
Aribah Naz1*, Uzma Badar1,Afsheen Arif2*, Zaid Qureshi1, Sondas Saeed1,Erum Shoeb1, Maqsood Ali Ansari1 and Obaid Yusuf Khan1
...onditions including high salinity and low pH. Ease of detection is one of the prominent features of these glowing organisms to be the center of attention of many researchers and scientists. The focus of the study is to characterize and identify local Gram negative luminescent bacterial strain isolated from gut of Nemipterus japonicus commonly known as Japanese threadfin bream fish.Isolation, temperature optimization for growth and luminescence, growth c...
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2,  Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
...periment, total 50 wound samples were collected from donkey wounds. All 50 samples were found contaminated with different bacterial organism, among all those 45, 35, 33, and 25 were recorded positive for S. aureus, E. coli, Shigella and Salmonella spp respectively. However, out of 50 sample, 34 (68%) were found to have different bacterial species and 16 (32%) were recorded wi...
Zengwen Huang1,2, WuReliHazi Hazihan2*, Baheti Bodai2, Kadyken Rizabek3, Nuralieva Ulzhan3, Omarova Karlygash4, Juan Zhang1 and Yaling Gu1*
...ted into YM sheep granulosa cells. The sequence of YM sheep INHα gene was obtained and uploaded on the NCBI database (accession number KP-113678.1). The INHα gene from YM sheep is 1109 bp long and translates to approximately 40 KDa protein. Q-PCR analysis showed that the plasmid pGenesil 10-3p-siRNA could interfere with the expression of INHα in granulosa cells to an efficiency of 83%, which was also confir...

Shahzad Malik* and Ayesha Khan

Gap Analysis of Farmers’ Capacity Building by Public and Private Agriculture Extension Sectors in Central Plain Valley of Khyber Pakhtunkhwa, Pakistan
...Peshawar, Mardan and Charsadda were selected purposively. In order to carry out the research, in the three districts of Peshawar, Mardan and Charsadda one tehsil was selected from each district i.e. Town-4 from Peshawar, Takhtbhai from Mardan and tehsil Charsadda from district Charsadda. Sample size for the present study was 270 respondents i.e. 90 respo...

Muhammad Ismail1, Tufail Ahmad2, Shamsher Ali2*, Shahid Ali1, Nur Ul Haq1 and Naveedullah3

Heavy Metals (Pb and Ni) Pollution as Affected by the Brick Kilns Emissions
...+18) soil and wheat leaf samples were collected in four directions. Samples were collected at varying distances (100, 200,300 meters) from chimneys. Similarly, samples of dust were also collected for analysis of heavy metals in available locations around the chimneys in 16 plastic buckets. They were placed 3 m high from ground. The laboratory analysis of wheat leaf samples showed that the ...
Zubair Ahmad1, 2, 4, Hamed A. Ghramh1, 2, 3,Khalid Ali Khan1, 2, 3*, Farhat Khan4 and Shujauddin5
...tion between aphid M. sacchari and their attending ants on sugarcane (Saccharum officinerum) was studied. The presence of ants, especially, Crematogaster subnuda Mayr. and Camponotus compressus adversely affects the parasitoid effectiveness of Lysiphlebia mirzai and Aphelinus desantesi. The aphids got 31% and 26.3% parasitism when attended by C. compressus and C. subnuda
Ishrat Perveen1, Yasar Saleem2 and Javed Iqbal Qazi3* 
...-eat (RTE) chicken kabab samples of four commercially available brands (K, S, D and B) were analysed for the simultaneous determination of HCAs i.e. 2-amino-3-methyl-imidazo[4,5-f] quinoline (IQ), 2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (MeIQx) and 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhlP) using HPLC-UV detector. Quantitative analysis of the control samples (only thawed at room tem...
Aamir Khan1, Rabia Noushin2, Mohammad Attaullah3, Shahid Naiz Khan1, Rafiqe Hussain4, Farman Ullah Dawar1, Faiz Ur Rehman1, Muhammad Ijaz1 and Kalim Ullah1*
...hyber Pakhtunkhwa. Blood samples (1500) were collected from clinically suspected cattle in span of one year. All blood samples were examined through microscope. Overall incidence of babesiosis was 54.80%.B. bovesshowed high prevalence (35.46%) than B. bigemina (19.33%). Females were more prone as compared to male cattle for both B. bovesandB. bigemina. Cattle having age less than two years ...

Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2

A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
....0% to household income (sale of milk 11% and sale of animals 10%). Relatively more proportion of females was recorded in livestock rearing (44.6%); followed by skilled labor (handicrafts: 28%), and agriculture (12.1%). 

...

Muhammad Qaisar Nawaz1, Khalil Ahmed1*, Ghulam Qadir1, Amar Iqbal Saqib1, Muhammad Rizwan1, Muhammad Faisal Nawaz1 and Imtiaz Ahmad Warraich2

Impact of Fertilizer and Planting Geometery on Garlic (Allium sativum L.) Yield in Saline-Sodic Soil
... garlic production under saline environment. The experimental design was split-plot with three repeats. Three planting geometry (10cm x 20cm, 15cm x 20cm and 20cm x 20cm) and four phosphorus levels i.e. (i) control (no phosphorus), (ii) P (60 kg ha-1) recommended dose (RD), (iii) 125% P of RD (75 kg ha-1) and (iv) 150% P of RD (90 kg ha-1) were used. Observations included were; plant height, bulb mass, bulb diameter, cloves bulb-1 and bulb yield. Data revealed...

Muhammad Ahsan Naseer1, Mehdi Maqbool1, Saima Rafiq2*, Noosheen Zahid1, Abdul Hamid1 and Syed Zulfiqar Ali Shah1

Comparative Analysis of Physical and Biochemical Attributes of Edible Fig (Ficus carica L.) Collected from Three Districts of Azad Jammu and Kashmir Located at Different Elevations
...Akhorbun) and Kotli (Sehnsa, Kotli city, Khoiratta)] of AJK. Samples of wild fig fruits were collected and analysed for physical (fruits weight, fruits diameter, neck length) and biochemical attributes (pH, titratable acidity, total soluble solids, total moisture, vitamin C, total phenolic and antioxidant activity). Results showed that wild figs obtained from diverse localities have different attributes in terms of physical as well biochemical properties. Maxi...

Amir Afzal, Muhammad Ijaz and Sayed Rashad Ali Shah*

Determination of Suitable Growth Stage for Application of Fungicide against Stripe Rust in Wheat
...notypes 16C038 and cv. Ihsan 2016 were selected for study. Difenoconazole 25% was treated @ 0.3 ml/L of water using Knapsack sprayer. Data regarding yield and yield parameters were recorded and analyzed. Significant difference was observed between treated and untreated plots at various growth stages when treated in susceptible genotype (Ihsan, 2016) and contrary in case of resistant genoty...

Khalil Ahmed1*, Amar Iqbal Saqib1, Abdul Rasul Naseem1, Ghulam Qadir1, Muhammad Qaisar Nawaz1, Muhammad Khalid2, Imtiaz Ahmad Warraich3 and Muhammad Arif4

Use of Hyacinth Compost in Salt-Affected Soils
... improving the health of salt-affected soil is a simpler technique. Therefore, present research work was carried out to appraise the efficacy of water hyacinth compost as an ameliorant for improving the deteriorated properties of saline-sodic soil. Treatments included were; T1, control, T2, gypsum @ 100 % GR, T3, gypsum @ 50 % of GR, T4, hyacinth compost @ 15 t ha-1, T5, gypsum @ 50 % of GR + hyacinth compost @ 5 t ha-1, T6,...

Adnan Arshad1*, Huma Qamar2, Ristina Siti-Sundari3, Zhang Yue1, Muhammad Zubair2, Muhammad Ali Raza4, Muhammad Habib-ur-Rehman5 and Lizhen Zhang1*

Phenotypic Plasticity of Spineless Safflower (Carthamus tinctorius L.) Cultivars in Response to Exogenous Application of Salicylic Acid under Rainfed Climate Conditions
...ing changing climate, as safflower has gained prominence as an oilseed industry due to its hardiness in nature. A two-factor factorial experiment using completely randomized block design (RCBD) followed by five spineless safflower cultivars and five concentrations of salicylic acid (SA) in three replications was conduc...

Iram Bilqees1, Yasir Iftikhar1, Mustansar Mubeen1,2*, Qaiser Shakeel3, Ashara Sajid1, Zahoor Hussain4, Aqleem Abbas2, Muhammad Aamir Sohail2, Judith Jeruto Kiptoo5 and Shehzad Iqbal2

Use of Nutrients and Plant Extract to Manage Okra Yellow Vein Mosaic Disease (OYVMD) in Sargodha, Punjab, Pakistan
...ify;">Okra yellow vein mosaic disease (OYVMD) has been a menace to okra cultivation wherever grown in the world. This trial was carried out to monitor the disease severity, incidence and its management through different nutrients and plant extract. Plant extract and multi-nutrients were used to manage the disease to avoid the hazardous effect of chemicals on human health and being the eco-friendly. Four varieties of okra viz., Indian Rachna, Sabz Pari, Desi Bh...

Olugbenga Omotayo Alabi1*, Ayoola Olugbenga Oladele2 and Ibrahim Maharazu3

Profitability Analysis and Marketing Efficiency of Soyabean (Glycine max) Value Chain among Actors in Abuja, Nigeria
...eters of soyabeans were: sales price instability, high cost of transportation, storage problems, lack of credit facilities, and bad road infrastructures. The retained components observed explained 93.24% of the variations in the component retained in the principal component model. The study recommends that marketers should develop new marketing strategies that will increase their profit margin. The sales price instability as...

Hafsa Naheed* and Hidayat-Ur-Rahman

Genotype by Environment Interactions of Vegetative Growth Traits of Bread Wheat Genotypes

Abid Khan*, Mukhtar Alam, Yousaf Jamal and Hidayat Ullah

Appraisal of Yield and Yield Components of Wheat with Integrated Management of Nitrogen Sources
... Winter wheat variety Pirsabak-2015, as a test crop, was sown on 12th November at same piece of land following same randomized layout each year. Farm Yard Manure (FYM) and Poultry Manure (PM) were used as organic while Urea as inorganic sources of nitrogen. Treatments were N1 (Control), N2 (all added N from Urea), N3 (all added N from FYM), N4 (all added N from PM), N5 (½ of added N...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
...luding diverse type of Fusarium species. Molecular approach is a useful technique for the identification of wilt complex pathogens. The study conducted to identify the polygenetic association of the pathogens of chickpea wilt complex. Universal Rice Primers based diversity analysis indicates high polymorphism within the collected wilt isolates. Primers ITS 1 and ITS 4 generated internal transcribed spacer fragments that were...
Safina Naz1, Syed Atif Hasan Naqvi2*, Bushra Siddique3, Muhammad Asif Zulfiqar4 and Abdur Rehman5 
 
Exogenous Application of Selected Antioxidants and Phyto Development Directors Influenced the Development, Output and Biochemical Attributes of Tomato (Lycopersicum esculentum Mill.)
...m). This was followed by salicylic acid (200 ppm). Leaf number was significantly lesser in the plants grown in control. Significantly greater fruit count /plant, length of fruit including sole fruit freshness and dry mass were recorded with GA3(100 parts/million) and salicylic acid (200 ppm) provision and lower values for these parameters were recorded with control. Fruit weight for single plant and output of fruit per hecta...

Rana Shahzad Noor1,2, Fiaz Hussain2, Irfan Abbas3, Muhammad Umair2, Abu Saad2, Muhammad Umar Farooq4 and Yong Sun1*

Assessing Sustainability of Rainfed Wheat (Triticum Aestivum) Production under Various Soil Tillage Systems: An Energy and Economic Analysis
...T) on wheat yield, fuel usage and energy use efficiency under rainfed agriculture systems for Chakwal-50 wheat cultivator at Koont research station of PMAS-Arid Agriculture University, Rawalpindi during 2018 and 2019 wheat seasons. The study treatments were evaluated in term of energy analysis and economics of wheat production. Highest energy inputs obtained were fertilizer, seed and fuel + oil energies in all tillage systems for wheat sowing, respectively. Re...
Shahid Ali*, Aijaz Ali Channa, Waqar Ahmad, Rafia Akram and Imbasat Yousaf
Muhammad R. Khan1, Bushra A. Rakha1,* and Muhammad S. Ansari2
... conservation efforts to save from becoming extinct in most of its range. The most abundant species of river swat was Schizothorax plagiostomus while the least abundant species was Schizothoraicthys macropthalmus. However, all of the species of genera Schizothorax and Schizothoraicthys facing drastic decline in their distribution range and within River Swat due to introduction of exotic salmons an...

Muhammad Shakeel1*, Mudussar Nawaz1, Zahid Naseer1, Muhammad Fiaz1, Asghar Khan1, Muhammad Imran Khan1, Awais Ur Rehman1, Ahmad Yar Qamar2 and Ali Raza3

Caprine and Ovine Serological Evidence of Brucellosis in Five Districts of Punjab, Pakistan
...n. A total of 1239 serum samples were collected from male (n=73) and female (n=1166) different sheep (n = 865; Pak-Karakal, Thalli, Lohi, Kajli and non-descript type) and goat (n = 374; Teddy, Beetal and non-descript type) breeds of variable age groups (0 months to 4years). All the serum samples were analyzed using Rose Bengal Plate Test (RBPT) and seropositive samples were further submitt...
Murtaza, Shahid Ali*, Syed Attaullah Shah, Mehran Ahmad, Irfan Ullah and Jahangir Khan
Adoption of Hybrid Maize Technology in District Mardan of Khyber Pakhtunkhwa, Pakistan
...Pakhtunkhwa, Pakistan. A sample of 255 respondents in the study area was randomly chosen using a multistage random sampling technique. The logit model was used to measure the probability of adoption of hybrid maize technology. The estimated results of the model revealed that younger farmers were comparatively more adoptive of the hybrid maize technology. Education level, number of adult males involved in agriculture and land...
Arif Alam1*, Faridullah2, Ikram Shah1, Shahzad Khan3, Noor Elahi1 and Ehsan Inamullah1
Estimation of Farm Level Technical Efficiency in Maize Production at the High Mountain North Region of Pakistan
...lected with an aggregate sample of 112 maize crop growers. Both primary and secondary data were collected from different sources. To measure technical efficiency, the stochastic frontier production function along with technical efficiency model was employed. Data was analyzed through statistical software Frontier 4.1, because of its appropriateness in efficiency related assessments. Result shows that the factors of low yields of different pea

Inam Ullah1, Mahfooz Khan2*, Munir Khan2, Himayatullah Khan2 and Azra3

Factors Affecting Different Wheat Varieties Yield in District Peshawar, Khyber Pakhtunkhwa, Pakistan: A Comparative Analysis
...results show that for Pirsabaaq-5 wheat variety, the effect of (weedicides) was insignificant. While for Pirsabaaq-8 wheat variety irrigation was found insignificant and for Pirsabaaq-13, wheat variety the effect of seed was insignificant. It was observed that majority of the farmers were illiterate and do not apply the recommended amount of inputs due to its high prices/lack of availabili...

Dilshad Ahmad1* and Muhammad Afzal2

An Empirical Analysis of Economic Efficiency and Farm Size of Cotton Farmers
... technical efficiency of samples of 240 small, medium and large-scale cotton farmers was estimated using Stochastic Frontier Approach of district Rahim Yar Khan Punjab, Pakistan. Empirical findings of the study indicated that medium farm size cotton farmers were more allocative, technically and economically efficient (0.90, 0.94, 0.85) rather than small farm farmers (0.79, 0.92, 0.76) and large farm farmers (0.70, 0.97, 0.68). Farmers schooling years, formal c...

Amit Sharma1* and Pankaj Sood2

Sonographic Assessment of Ovarian Follicular Dynamics during Breeding and Non-Breeding Season in Gaddi Goats
...ne, n=11) seasons. Blood sampling on alternate days was done during both the seasons for serum progesterone (P4) estimations. Follicular growth was characterized by presence of three to five numbers of follicular waves in both seasons. Significantly higher (P<0.01) number of follicular waves (4.0±0.21 versus 3.18±0.12), number of follicles at wave emergence during second (2.85±0.26 versus 1.77±0.14; P<0.01) and third wave (2.5...

Abdiaziz Idiris Mohamud1, Yonis Abukar Mohamed2, Osman Sheikh Ali Jama3, Pravin Mishra4* and Mohamed Idiris Mohamed5 

Prevalence and Major Pathogens Associated with Clinical and Subclinical Mastitis in Dairy Camel (Camelus dromedarius) in Benadir Region of Somalia
...ted microorganisms as causal agents of mastitis. The prevalence of this study was measured by using California mastitis test (CMT). Milk samples were collected from the Deyniile District, Benadir Region of Somalia. The overall prevalence of mastitis was 16.66% (7.93% on the quarter basis), the prevalence of clinical and subclinical mastitis was found to be 22.78% and 9.85% on the animal basis and 9.37% and 6.15% on quarter b...

Md. Aminul Islam1*, A.N.M. Aminoor Rahman2, Mohammad Shah Alam3 and Md. Taimur Islam4

Gastrointestinal Nematodiasis in Quail in Bangladesh
...sh. A total of 200 fecal samples were collected from quail farms from various regions of Bangladesh. The fecal samples analyzed by direct smear methods and nematodes identified by the presence of characteristic eggs in the feces. Five types of nematode eggs found on different farms. The overall prevalence of gastrointestinal nematodiasis were (17.5%), which could consider relatively higher. The highest rate of infection foun...

Malik Gul Hassan Awan, Shahzada Sohail Ijaz, Muhammad Ansar, Adeel Anwar, Umber Abbasi and Hashmat Mahmood

Influence of Foliar Application of Mineral Nutrients on Biological Nitrogen Fixation of Rainfed Soybean
..., Cu, Zn, P and Ca) in a sandy loam soil (Typic Ustorthents). The spray applied 0.7 g ha-1 Mn, 0.7 g ha-1 B, 0.7 g ha-1 Cu, and 1 g ha-1 Zn besides 59 g ha-1 P, and 27 g ha-1 Ca. Foliar application enhanced the nitrogen fixation in all the soybean varieties. Increase was 98% in NARC-2, 84% in Rawal-1 and 70% in Ajmeri-1. The highest N2 fixation of 68 kg ha-1 was observed in NARC-2 with foliar application of nutrients. Nodule numbers increased from 0.12 plant-1...
Tabassum Yaseen1*, Shehzad Ahmad1, Khushnood Ur Rehman2, Fayaz Asad1, Abdul Waheed1, Rani Gul3, Hussain Gulab4 and Naveed Akhtar2
Arbuscular Mycorrhizal Fungal Spore Density and Root Colonization in Weeds of Carrot field at Charsadda, Pakistan
...or weeds of District Charsadda. In the present study roots and rhizospheric soil of 15 weeds species belonging to 12 families were collected from Carrot field of District Charsadda and was investigated for the sporulation and root infections types. From the recorded results the highest spore density was found in Melilotus indica having spore number 276 which is followed by Malva neglecta and Sonchus asper having spore number...

Rao Wali Muhammad1, Hafiz Muhammad Wasif Ali2*, Amir Hamza1, Muhammad Qadir Ahmad2, Abdul Qayyum2, Waqas Malik2 and Etrat Noor2

Estimation of Different Genetic Parameters in Various Safflower (Carthamus tinctorius L.) Genotypes under Field Condition
...d yield per plant of 200 safflower accessions. Analysis of variance and principal component analysis were carried out to estimate the extent of variability for studied parameters and to partition the germplasm into various cluster groups on basis of their mean performance. A value of 74% was indicated by the first three PCs, which showed the highest variability between studied parameters. Eigen values >1 contributed variability between the genotypes under f...

Muhammad Arsaln1, Sair Sarwar2*, Rizwan Latif1, Junaid Nawaz Chauhdary3, Munazza Yousra2 and Shahbaz Ahmad2

Effect of Vermicompost and Microbial Inoculants on Yield, Soil Fertility and Economics of Wheat under Rainfed Conditions

Rabab Rafaqat1, Habib Ahmed Rathore1, Tariq Masud2, Imran Hayat1* and Imtiaz Hussain1

Determination of Different Chemical Constituents of Fruit, Leaves and Oil of Olea cuspidata (Wild Olive) Grown at Rawalakot, Azad Jammu and Kashmir
...ide value, iodine value, saponification number and unsaponifiable matter. Results indicated that fruit and leaves of wild olive grown at Rawalakot Azad Jammu and Kashmir had moisture content (48.33±1.6 and 53.33±1.4 %), crude fiber (4.40±0.3 and 10.0±0.9 %), crude protein (0.80±0.02 and 8.20±0.80 %), total oil (34.43±1.1 and 1.00±0.6 %), ash content (1.71±0.2 and...

Mohammad Javad Golmohammadi1, Hamid Reza Mohammaddoust Chamanabad1*, Bijan Yaghoubi2 and Mostafa Oveisi3

GIS Applications in Surveying and Mapping of Rice Weeds in Guilan Province, Iran
...nce. The Langarud and Rudsar regions each with 47 species and Anzali and Shaft with 25 species (with 71.2 and 37.9 percent, respectively) had the highest and lowest species diversities, species diversity in 2015 was more than 2014 and 2016 years. The frequency of weed species in Guilan rice farms in 2014-2016 was 3.38, 3.87 and 4.82percent, respectively. Cyperus esculentus and P. distichum were the most abundant species with 9 and 8 plants/m2, respectively. Ec...

Joseph Anejo-Okopi1*, Obinna Oragwa Arthur2, Ocheme Julius Okojokwu1, Sarah Joseph1, Geoffrey Chibueze1, Joshua Adetunji1, Joseph Ameh Okwori3, David Ochola Amanyi4, Otobo I. Ujah5 and Onyemocho Audu6

Seroprevalence of Rift Valley Fever Virus Infection among Slaughtered Ruminants in Jos, North-Central, Nigeria
...-Central, Nigeria. Blood samples were collected from 100 livestock (cattle and goats) at selected slaughter locations in Jos Metropolis. Questionnaires were administered to obtain information on animal species, sex, and localities of origin. The blood samples were screened for RVFV antibodies using competitive Enzyme Linked-immunosorbent Assays (C-ELI

Iram Sharif1*, Amna Nazir1, Eram Shahzadi2, Shahid Munir Chohan3 and Ghulam Sarwar4

Influence of Sowing Dates on Cotton Growth (Gossypium hirsutum), Yield and Fiber Quality
...ton Research Station, Faisalabad, during the year 2018-19. The objective of the research was to evaluate the sowing date effects on growth, yield parameters and fiber quality of cotton and genotypic assessment in two sowing dates. The set of six genotypes was sown in two different dates viz., 10th April and 10th May in split plot design with three replications by allotting sowing dates in main plot and varieties in sub-plots. The results indicated that crop so...
Huma Abbas1, Muhammad Azhar Iqbal2, Muhammad Kamran3, Muhammad Umar Shahbaz3*, Haseeb Ullah Kamber1, Nazir Javed1, Muhammad Junaid1, Hira Abbas4 and Muhammad Ehetisham ul Haq3
Evaluation of Advanced Mung Bean Germplasm against Cercospora Leaf Spot and its In-vitro Management by Different Fungicides
...and with environmentally safe fungicides is a cost-effective approach. The present study was aimed to find the resistant germplasm against the disease and to evaluate the relative efficacy of different fungicides against the C. canesens in lab conditions. Twenty varieties/lines were screened against Cercospora Leaf Spot disease in field conditions. No line/ variety was found immune and only one line (16051) was resistant against this disease. Five different fu...

Shahid Nadeem1*, Taj Naseeb Khan1, Mushtaq Ahmad1, Adnan Younis2 and Iram Fatima3

Influence of Plant Growth Regulators by Foliar Application on Vegetative and Floral physiognomies of Gladiolus
...bberellic acid (GA3) and salicylic acid (SA). The results revealed that the application of GA3 and SA had significant influence on vegetative as well as floral traits of gladiolus. The maximum numbers of days (7.90) for the sprouting of gladiolus corms were counted with 1mM of

Attiq ur Rehman1,2*, Iftikhar Hussain Khalil3 and Ihtisham Ali4

Genetic Diversity and Traits Association in Tetraploid and Hexaploid Wheat Genotypes in Khyber Pakhtunkhwa Province of Pakistan
...eat verities, Janbaz, Barsat and Shahkar outperformed others in terms of 1000-grain weight, grain yield, and harvest index, respectively. These genotypes are recommended to be further tested at multi-locations to check for wider adaptability and a possible use in future wheat breeding programs in the area.

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Zahir Shah1*, Saima Munawar1, Ihsan Ullah2, Taif Shah3, Haq Aman Ullah1, Saqib Nawaz1, Farhan Anwar Khan1 and Manoj Kumar Shah4

Combined Physico-chemical and Analgesic Effects of Electroacupuncture Plus Clonidine in Goats
...oup received 0.9% normal saline, 2nd, 3rd and 4th groups were treated with 10, 15, and 20µg/kg of clonidine respectively, while 5th and 6th groups were treated with EA and EA plus 10µg/kg of clonidine, respectively. Pain threshold, cardio-respiratory effects, rectal temperature, hematological and biochemical variables were assessed in all groups. Clonidine at the dosage of 20µg/kg increased the pain thresho...

Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...as carried out in the biosafety level 3 Microbiology Lab, Faculty of Life Sciences, University of Bradford, Bradford, UK. Two different concentrations (low, 5 mg/ml and high extracts, 300 mg/ml) of these plants were evaluate against two different bacterial strains i.e. Bacillus subtilis and E. coli using disc diffusion method to uncover antibacterial potential of the plants extracts. The antibacterial investigation was performed in three replicates using disc ...

Shahzad Khan*, Munir Khan, Inayatullah Jan, Mahfooz Khan and Fida Muhammad Khan

Determinants of Sugarcane Yield in District Charsadda, Pakistan
...ane crop in district Charsadda in the year 2018. Three villages viz. Kamran Kalay, Aspandehri, and Sarfaraz Kalay were selected purposively. Primary data were collected with face to face interview method from 41 randomly selected sugarcane growers through structured questionnaire. The selection of farmers was based on proportional allocation technique in the selected villages. For data analysis, profit margin, gross margin and Cobb-Douglas production function ...

Muhammad Zamin1*, Anees Muhammad1, Ibadullah Jan1, Hidayat Ullah1, Shahen Shah2, Muhammad Amin3 and Haroon Ur Rashid4

Production of Tuberose (Polianthes tuberosa L.) as Affected by Bulb Size and Planting Medium
...found in compost amended sandy soil. As far as the effect of bulb size is concerned, maximum days to flowering (97.44), plant height (43.29cm), number of leaves (24.54), number of florets (19.17), bulblets plant-1 (24.30) and bulblet diameter (2.08 cm) were produced by growing large sized bulbs.

...

Muhammad Zamin1*, Anees Muhammad1, Ibadullah Jan1, Hidayat Ullah1, Shahen Shah2, Muhammad Amin3 and Haroon Ur Rashid4

Production of Tuberose (Polianthes tuberosa L.) as Affected by Bulb Size and Planting Medium
...found in compost amended sandy soil. As far as the effect of bulb size is concerned, maximum days to flowering (97.44), plant height (43.29cm), number of leaves (24.54), number of florets (19.17), bulblets plant-1 (24.30) and bulblet diameter (2.08 cm) were produced by growing large sized bulbs.

...
Ateeq Ahmad1, Umar Hayat2, Shahid Ali2*, Hazrat Bilal3, Naveed Ali2, Khan Mir Khan4 and Khalid Khan4
Descriptive Analysis of Water Resources in Pakistan: A Perspective of Climate Change
...gh economic resources to satisfy the basic needs of masses. Furthermore, the future of Himalayan glaciers at risk due to emerging climate change in the region. Pakistan is located in rid region where the issue of water security is forefront problem particularly for agricultural sector. While its use in various sectors like industrial and urban push the country further into water crises. The purpose of present research is to highlight the significance of water ...
Muhammad Zahid Mengal1, Hamida Ali2, Raheela Asmat3, Muhammad Naeem1,4, Ferhat Abbas1, Abdul Samad1, Mohammad Zahid Mustafa1, Jannat Raza2 and Tauseef M. Asmat1*
...up>® MTB/RIF assay.In total, 2300 clinical specimens were collected from suspected TB patients at Fatima Jinnah General and Chest Hospital Quetta, Pakistan between January and August 2017. These specimens were analyzed by GeneXpert® MTB/RIF assay. The data was statistically analyzed using SPSS software. Out of 2300 clinical specimens, M. tuberculosis was positive in in 899 (39.1%) cases by Gen...
Sajad Ali1*, Naeem Ur Rehman Khatak1, Iftikhar Ahmad2, Jangraiz Khan3 and Azra4
Impact of Socioeconomic Factors and farm Size on Wheat Productivity: A Case Study of District Peshawar, Pakistan
...from district Peshawar.A sample of 200 (160 large and 40 small) wheat growers weretaken from twelve selected villages of Peshawar. An unpaired t-test was used to test the differences in the socioeconomic factors and farm sizes on the wheat production. Chow test were used for structural differences and dummy variable were introduced in the production function to test any significant productivity differentials (if any) between two farm groups. A significant diff...
Muhammad Yaseen1*, Xu Shiwei2, Yu Wen2, Ejaz Ashraf1, Muhammad Luqman1, Muhammad Ameen3 and Sadia Hassan4
Farmers Behavior to Participate in Public Sector Agricultural Extension Services: Comparative Analysis of Pakistan and China
...r this purpose, a random sample of 160 households from four districts of Punjab province of Pakistan was selected, and another random sample of 122 households selected from the six villages of Huailai County of Hebei Province in China. Data were collected using the interview method with the help of validated and expert reviewed interview schedule. The results revealed that the household head’s average age is 47 years i...
Sri Nuryani Hidayah Utami*, Andin Muhammad Abduh, Eko Hanudin and Benito Heru Purwanto
Study on the NPK Uptake and Growth of Rice under Two different Cropping Systems with different Doses of Organic Fertilizer in the Imogiri Subdistrict, Yogyakarta Province, Indonesia
...cropping systems was the same, which is a mixture of cow manure and neem compost (1:1) at doses of 0, 3, 6, and 9 ton ha-1. The research data are presented in tables and graphs to show the effects of modification of the rice cropping system on the nutrient uptake. The results showed that the Jarwo 2:1 provided a higher N, P, K uptake compared to the Grid system. The provision of PFA had an impact on increasing the number of tillers, the number of productive ti...

Malik Abdul Rehman1, Shafqat Ali1, Muhammad Babar Shahzad Afzal1, Muhammad Nawaz Khan1 and Mujahid Ali2*

Efficacy of Chemicals and Botanical Extracts to Control Citrus Canker on Kinnow in Sargodha Region

Muhammad Imran1,2*, Shahbaz Talib Sahi1, Muhammad Atiq1 and Amer Rasul3

Brown Leaf Spot: An Exacerbated Embryonic Disease of Rice: A Review
...ist are considered to be safe, eco-friendly, cost-effective economically and biodegradable. The use of plant activators as another new strategy that activates the defense system of plants and reduces the disease. The plants which are scarce nutrients are more prone to disease as compared to nutrient deficient. The pathogen damage is compensating by a specific nutrient that reduces the disease through tolerance. Good manageme...

Zulnorain Sajid1*, Muhammad Ramzan2 and Noreen Akhtar1

Foraging Behavior and Pollination Ecology of Bumble Bee and Honey Bee in Pakistan; A Review
...tion to burst the pollen sac, high speed to flower visitation at low temperature and solitary colony structure. In northern areas of Pakistan bumblebees are natural pollinators. The various products of honey bee especially honey, royal jelly and bee venom used for the treatment of various diseases like cardiac diseases, hypertension and many others problems. These are the main source of income for entomologist and agriculture graduates. There are several facto...

Md. Sahidur Rahman

Origin and Spillover of Coronaviruses: Prospects of One Health Action
...and regulating proper biosafety, biosecurity, and risk communication to facilitate strong disease prevention and control strategy by which we could prevent the next coronavirus outbreak in humans.

...

Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*

Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...rescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity is influenced by antibody production methods. Therefore, the aim of this review was to describe various antibody production methods, which might be used in developing DFA assay to diagnose H9N2 infection in humans or chickens, and we discussed avi...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...lo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PV...
Asmat Ullah1,2, Shahid Iqbal1,2 and Furhan Iqbal2*
.../i> leaf extract or with saline solution (0.9% NaCl) for 17 days. Behavioral observations were made by applying a series of neurological tests (Rota rod, Elevated plus maze, Light and dark box, Open field, Novel object and Morris water maze test). It was observed that Bauhinia variegata’s leaf extract supplementation improved neuromuscular co-ordination in female albino mice during rota rod test (P = 0.002), had more rotations (P = 0.02) and clock...
Gulzhan Aubakirova1*, Zhanat Adilbekov2, Assylkhan Inirbayev2, Assel Zhamanova2 and Aibar Akhmetov3
...khstan, and assesses the safety of fish. It was established that the hydrochemical regime of the water bodies was within the permissible concentration, however, a decreased permanganate oxidation was noted, which indicates a satisfactory state of water quality. Widespread infection of fish with postdiplostomatosis was revealed in assessing fish safety; in rare cases, diplostomosis and ligu...
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
Asim Faraz*
...a camel calves of almost same weight and age were divided randomly into three groups each having 3 ♂ and 3 ♀ reared under intensive (IMS), semi-intensive (SIMS) and extensive (EMS) management systems. The calves in first group reared in IMS were fed @ 1 kg concentrate having 18% CP and 2.41 Mcal/kg energy along with gram crop residues ad libitum, the second group calves reared in SIMS were allowed grazing/browsing for 8-10 hours and fed gram crop re...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...>Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 billion per mL conc., >60% motility) and cryopreserved using standard procedures. Percent sperm motility, liveability, plasmalemma integrity and viabil...

Joseph Anejo-Okopi1*, Dorcas Yilger Gotom1, Noble Allison Chiehiura1, Julius Ocheme Okojokwu1, David Ochola Amanyi2, John Otumala Egbere1, Joshua Adetunji1, Otobo Innocent Ujah3 and Onyemocho Audu4

The Seroprevalence of Zika Virus Infection among HIV Positive and HIV Negative Pregnant Women in Jos, Nigeria
... done using a commercial sandwich enzyme-linked immunosorbent assay (ELISA) kit to qualitatively analyze Human Zika virus IgG (ZV-IgG) in human serum. A total of 90 pregnant women was recruited, and the overall seroprevalence was (14.4%) for Zika virus (20.0% HIV positive and 8.9% negative) IgG antibodies. A significant association was found with age cat...

Dalal H. Sary and Rama T. Rashad*

A Comparative Study on the Impact of Compost, Humate, and Silicate on the Nutritional Characteristics of Calcareous Soil Cultivated by Soybean
...il may be enriched by versatile advantages upon application of K-H and K-Si but the compost is still the most effective.

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Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...tal of 1260 poultry meat samples were collected from four different regions of Khyber Pakhtunkhwa province and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni were isolated using enrichment and plate media followed by confirmation through multiplex PCR. Isolates were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence...

Muhammad Nauman, Unsar Naeem-Ullah*, Mehreen Hanif, Hafsah Ghaffar, Muhammad Shahid and Syed Haroon Masood Bokhari

Management of Tribolium castaneum (Herbst) and Rhyzopertha dominica (Fabricius) by using Microwave Oven
... using microwaves can be safe option than chemical control. Therefore, in this study a digital microwave oven of 50 Hz is used to determine the mortality of Tribolium castaneum (Herbst) and Rhyzopertha dominica (Fabricius) adults. Grain samples of 20 g in each petri dish were infested with both adults and exposed to microwaves for 0, 10, 20, 30 and 40 sec. For germination determination 25 seeds from each treatment were selec...

Sajid Amin Sarmad1, Muhammad Zeeshan Majeed1*, Muhammad Luqman1, Muhammad Asam Riaz1, Sohail Ahmed3 and Sylvain Nafiba Ouédraogo2

Development and Laboratory Evaluation of a Slow Release Formulation of Fipronil against Subterranean Termites (Odontotermes obesus Rambur)
...tment after 48 h of bioassay. It was concluded that fipronil can be used as a slow acting toxicant by formulating it with some cellulose-based material such as powdered maize cobs in order to attract and kill the subterranean termites.

...

Ghulam Akbar1*, Ali Ahmad2, Neha Arooj1, Muhammad Anjum Zia1, Aamna Rafique1, Sania Riasat1, Mohsin Raza1, Mahpara Qamar1, Shahneela Nusrat1 and Shakila Hanif1

Critical Update for the Treatment of Anemia by using Advanced Genome Editing Crispr Cas Technology
... provides potential for usage of gene editing technique for different types of anemia. In evaluation we defined currently developed genome editing device update used for the remedy of anemia, its mechanism of movement and sickle cellular mutation corrections.

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Muhammad Ilyas1,2*, Imran Khan2*, Muhammad Umer Chattha2, Muhammad Umair Hassan2, Muhammad Zain3, Wajid Farhad4, Sana Ullah1, Ateeq Shah5, Safeer Ahmed6, Bismillah Khan1 and Muhammad Adeel1
Evaluating the Effect of Zinc Application Methods on Growth and Yield of Wheat Cultivars
...rains/spike (39.18), thousand grain weight (40.27 g), biological (11.42 t ha-1) and grain yield (5.12 t ha-1), whereas the minimum LAI, CGR, tillers (261.89 m2), spike length (7.74 cm), spikelet/spike (15.11), grains/spike (32.76), thousand grain weight (33.99 g), biological yield (10.55 t ha-1) and grain yield (4.35 t ha-1), were noticed in control. Among the wheat cultivars, Galaxy-2014 performed better than the Punjab-201...
Rubina Munir1,2*, Muhammad Zia-ur-Rehman3, Muhammad Makshoof Athar1, Noman Javid1,4, Ayesha Roohi2 and Syeda Fatima Razavi2
...e been prepared by condensation of substituted 2-chloroquinoline-3-carbaldehydes with sulphonamide substituted benzoic acid hydrazides. Structures of the Schiff bases have been confirmed by spectral techniques including FT-IR, 1H-NMR, 13CNMR, and mass spectroscopies. The purity of compounds has been established by elemental analysis. Free-radical scavenging activity of these compounds has been carried out by DPPH as<...
Ammara Gull-E-Fareen1, 3, Imran Bodlah1*, Muhammad Tariq Rasheed1, Yasir Niaz2, Muhammad Adnan Bodlah2, Muhammad Asif4 and Nasir M. Khokhar5
...1855), Myrmica aimonissabaudiae Menozzi, 1939, Tapinoma melanocephalum (Fabricius, 1793) and Tetraponera allaborans (Walker, 1859). A lot of surveys were conducted during 2015-17 for the collection of ants associated with aphids on different host plants in different localities of Pothwar region. As a result of surveys, ant-aphid new mutualistic trophic associations were determined like Camponotus compressus with 12, Formica fusca...
Rida Younas1*, Tamseela Mumtaz1, Nabeela Roohi2 and Muhammad Amir Iqbal3
...f IL-6 and IL-8 in serum samples were evaluated by Enzyme-linked immunosorbent assay. Correlation between serum inflammatory cytokines (IL-6 and IL-8) level and post predicted FEV1%, FVC% and FEV1/FVC was analyzed. Results of serum IL-6 and IL-8 showed significant difference among control and GOLD stages II, III and IV (both P< 0.05). The level of IL-6 was highest in GOLD stage II and lowest in stage III significantly (P&...
Muhammad Umer Khan1,2*, Muhammad Farooq Sabar1, Atif Amin Baig3, Arif-un-Nisa Naqvi4 and Muhammad Usman Ghani1
...has been adopted as a versatile genetic marker all over the globe and provides a unique maternal ancestry portrait of a person’s genetic pin code. Overall knowledge of mtDNA profiles of worldwide populations can benefit population genetics and forensic sciences. Consequently, this study was designed to establish the mtDNA profiles of the Shin ethnic group in Gilgit-Baltistan, the northern most territory of Pakistan. Phlebotomy was performed for a total o...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1* and Sajida Naseem2

...region of CO1 gene of 64 samples was amplified. The sequences of 658 base pairs were recovered from 62 samples, representing 7 families, 20 genera and 27 species. Araneidae was the most dominant familyfollowed by Salticidae, Oxyopidae, Clubionidae, Tetragnathida, Thomisidae, Mitergidae and Lyc...

Asma Rashid Tariq1,2*, Saadia R. Tariq2, Tariq Mahmud1 and Misbah Sultan1*

Selective Capture of CO2 from Mixture of Different Gases using Cellulose Acetate, Polyimide, Polysulfone and some other Polymers based Mixed Matrix Membranes: A Review
...sity, polyurethane, chitosan and many more. Likewise, along with other fillers, Metal organic frame works have also been tested as additive for the mixed matrix membranes as they offer several advantages over other fillers. This review majorly presents use of three polymers viz. cellulose acetate, polyimides and polysulfone, for fabrication of mixed matrix membranes. All of these polymers are of utmost industrial importance in the field of separation. Further ...
Hassam Ud Din1*, Huma Rizwana1, Rameez Raja Kaleri2, Kaleem Ullah2 and Muhammad Din2
Evaluation of Production Performance and Marketing of Small Ruminants in District Dukki, Balochistan
... Nana Sahib, Sadar and Nasar Abad of district Dukki of Balochistan. The sample size was composed by producer (50), wholesalers (50) and retailer (50) for convenient sampling contribution of each selected producer involved in the business area. The result showed that in the study area (60.00%, 24.00% and 16.00%) farmers have kacha, pacca and semi pacca ho...

Muhammad Nazar1, Chaudhary Muhammad Ayyub2, Mujahid Ali3*, Malik Abdul Rehman4, Shoib ur Rehman5 and Saqib Ayyub2

Amelioration of Thermo-Tolerance Ability in Spinacia oleracea L. Cultivars by Foliar Application of Chitosan
...liar application of chitosan was a very significant tool. Three cultivars of spinach (All Pure Green, Desi Local, and Lahori Palak) were grown at normal temperature 18/20°C (day/night temperature) and then after four weeks after emergence 40/32°C (day/night temperature) was given by gradually enhancing 2°C per day. After one week of heat stress (40/32°C), four treatments (25, 50, 75, 100 ppm) of chitosan were...
Amir Aziz1*, Mukkram Ali Tahir1, Noor-us-Sabah1, Ghulam Sarwar1 and Sher Muhammad2
Effect of Rice and Wheat Straw and K-Silicate Application on Maize Growth
...ve hybrid maize seed (Monsanto 234) were sown but only 3 plants were maintained after germination. The fertilizers like nitrogen, phosphorus and potassium (Urea, SSP and SOP) were provided to the plants according to their requirement and time. The agronomic practices needed for plants were done according to plant demand. At maturity, data regarding yield components were recorded. Soil sampling was performed in all pots to an...

Muhammad Ramzan1, Muneer Abbas1*, Sohail Abbas2, Niaz Hussain1, Muhammad Aslam1, Javed Anwar Shah1, Muhammad Irshad1 and Mudassar Khaliq1

Incidence of Different Aphid Species on Various High Yielding Wheat Varieties
...ties i.e. Sahar-2006, Faisalabad-2008, Punjab-2011, Galaxi-2013, Gold-2016, Fakhr-e-Bhakkar-2017 and Bhakkar Star-2018 the most susceptible wheat stage against the infestation of aphid was investigated. Exploring most susceptible wheat stage was conducted through most common aphid species, i.e., Rhopalosiphum padi, Schizaphis graminum, Sitobion avenae on different varieties. Results indicated that heading and dough stages (Standard Weeks 10 and 11) were most s...

Malik Abdul Rehman1*, Hafiz Muhammad Ehsan2, Tehseen Ashraf2, Zulfiqar Ali Gurmani3, Sajjad Khan3 and Mujahid Ali2

Effect of Growing Media on Plant Growth of Rough Lemon (Citrus jambhiri Lush.) and Poncirus (Citrus trifoliata)
...ss, compost, soil, silt, sand, sawdust and leaf manure) in 14 different combinations of growth media to check for plant growth of rough lemon and poncirus rootstocks. Five plants per treatment were growing in black polythene bags filled with the required media using a Completely Randomized Design with three replications. Data of stem length stem diameter, number of leaves, leaf area, and survival percentage was recorded. Che...

Muhammad Yaseen1*, Muhammad Luqman1*, Zahoor Hussain2, Usman Saleem3, Asif Nawaz1, Tahir Munir Butt4 and Muhammad Umer Mehmood1

Assessment of Knowledge Level and Information Sources of Vegetable Growers regarding Tunnel Farming in District Sargodha
... the study. The snowball sampling technique led us to have 8 off-season vegetable growers in Sargodha tehsil, 13 in Bhulwal, 27 in Bhera, 17 in Kot Momin, 9 in Shah pur, 11 in Sillanwali, and 5 in Sahiwal. This way a sample of 90 respondents was interviewed using a structured interview schedule. The content validity of the instrument was checked by the panel of experts and followed by the reliability test run through SPSS. D...
Hong Hu1, 2, Li Qian1, Yuanlang Wang1, Chaodong Wu1, Xiaodong Zhang1, Yueyun Ding 1*, Li Wang1, Xudong Wu1, Wei Zhang1, Dengtao Li1, Jian Ding1, Min Yang1 and Zongjun Yin1*
...ction, biosynthesis of unsaturated fatty acids, fatty acid metabolism, regulation of lipolysis in adipocytes, glycerolipid metabolism, etc. The results of differentially expressed genes from sequence were highly reliable by qRT-PCR confirmation. The present work will help understanding of the different mechanisms involved in fat deposition between lean and obese pigs.
...
Shakeel Ahmed* and Sarwat Jahan
...ugh a cannula affixed in saphenous vein. Blood samples were collected individually 60 minutes before and 120 minutes after NMS administration at 15 minutes intervals. Plasma T and GH concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly (P<0.001) decreased plasma T levels but it did not cause any significant (P>0.05) change in plas...

Syeda Farzana Bibi* and Siraj ud Din

Unraveling the Bioherbicidal Potential, Elemental Analysis and Nutritional Evaluation of Crataeva adansonii Dc Leaf and Bark
...oncentration, and at the same concentration, the bark extract inhibited the frond growth by 40%. The pharmacognostic evaluation of this medicinal drug will be supportive in the detection of adulteration and quality control. Due to the herbicidal potential, it can be used in the agricultural industry. For future studies, it is suggested to understand the mode of action of its active compounds through future studies.

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Taqi Raza1*, Sergio de Los Santos Villalobos2, Muhammad Shehzad3, Shakeel Imran4 and Derly José Henriques da Silva5

PGP Characterization of Rhizobacteria Associated with Apple Gourd (Praecitrullus fistulosus L.)
... Furthermore, for exopolysaccharide (EPS) test, 73% of isolated strains confirmed the EPS production. The conclusion revealed that almost all isolated bacterial strains exposed the growth-promoting abilities and may be used as bio-fertilizer to enhance crop production.

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Atef  Mohamed El-Sagheer
Status of Phytonematodes in a Main Commercial Banana Production of Upper Egypt
...>The fields of banana (Musa sapientum L.) cultivations were surveyed for the prevalence of phytonematodes in Assiut governorate at middle of Upper Egypt. The encountered phytonematodes were identified during the survey belong to sixteen genera comprising sixteen genera belonging to nine families viz., Criconemella, Criconemoides, Ditylenchus, Helicotylenchus, Heterodera, Hoplolaimus, Longidorus, Meloidogyne, Paratylenchus, P...
Taqiur Rahman1, Tabassum Yaseen1*, Ali Mujtaba Shah2, Samiullah3, Ghulam Jelani3, Gul Nawaz1,Qudratullah Kalwar2
...healing of wounds, Arisaema jacquemontii blume used as antidote, Artimizia annua used asanathematize also in gastric problems, Avena sativa L. used as a laxative and antiseptic (R1). 
Samreen Khan, Tabassum Ara Khanum, Nasira Kazi, Salma Javed* and Shahina Fayyaz
Description of Aphelenchoides acacia n. sp. and Aphelenchoides naurangiensis n. sp. (Nematoda: Aphelenchoididae) with Observation on Aphelenchoides saprophilus Franklin, 1957 from District Lakki Marwat, Khyber Pakhtunkhwa, Pakistan
... sp. retrieved from soil sample of kikar (Acacia nilotica L.), ber (Ziziphus muritiana L.) and paper flower (Bougainvillea spectabilis L.) and Aphelenchoides naurangiensis n. sp. recovered from soil samples of wheat (Triticum aestivum L.), thoroughly described and illustrated from District Lakki Marwat, Khyber Pakhtunkhwa, Pakistan. The new species namely Aphelenchoides acacia n. sp. belongs to the Group 2 of Aphelenchoides ...

 Majid Shahi Bajestani1, Esmat Mahdikhani Moghadam2*, Reza Aghnoum3 and Hamid Rohani2

Study of Plant Parasitic Nematodes and Description of New Record (Rotylenchus alius) Associated with Barley (Hordium vulgare L.) in Khorasan Razavi Province, Northeast Iran
...lgare L.) fields of Khorasan Razavi Province, Northeast Iran, during 2015-2017. A total of 120 soil and root samples were collected to examine the prevalence of Plant parasitic nematodes. In morphological and morphometrical identification 18 species were recorded belonging to nine genera. Among these species, Rotylenchus alius was found as a new record from Iran and Basiria gracilis, Boleodorus thylactus, Ditylenchus apus an...
Kui Zhang1,2, Ping Geng1, Sher Khan Panhwar3, Khadim Hussain Memon4 and Zuozhi Chen1,2,*
...d. Therefore, it is necessary to intensify the management of the fish resources, and take effective measures to control the fishing effort and catches in Pakistani waters. Moreover, we recommended developing the pelagic fishery resources, such as purpleback flying squid Sthenoteuthis oualaniensis in the Arabian Sea.
...
Muhammad Aamir Naseer1, Amjad Islam Aqib2*, Ambreen Ashar3Muhammad Ijaz Saleem1, Muhammad Shoaib4Muhammad Fakhar-e-Alam Kulyar1, Khurram Ashfaq1, Zeeshan Ahmad Bhutta1 and Shagufta Nighat5
...> at various cadre. Milk samples (n=250) from cattle (n=90) and buffalo (n=160) were aseptically collected from various dairy farms and put to biofilm detection and antibiogram. Based on collected data with statistical inferences, the study found 61.60% of S. aureus from subclinical samples, while 72.73% of S. aureus were positive for biofilm with uniform hike in samples from...

Kecheng Zhu1,2,3, Peiying He1, Baosuo Liu1,2,3, Huayang Guo1,2,3, Nan Zhang1,2,3, Liang Guo1,2,3, Shigui Jiang1,2,3 and Dianchang Zhang1,2,3,* 

...s of promoter activity assays show that Almyomaker expression is notably activated by two MyoDs. Transcriptional activity of the Almyomaker promoter was observed to dramatically decrease after targeted mutation of the MyoD1 M1 and MyoD2 M2 binding sites. In summary, MyoD1 and MyoD2 play an important role in the regulationof Almyomaker expression and may promote myoblast fusion during muscle development and growth by modulating Almyomake...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...ty was assessed by MTT assay. LDH leakage was determined using a LDH assay kit. Mitochondria membrane potential (MMP) was measured by flow cytometry. LC3 conversion and p62 expression were evaluated by western blot analysis. MTT assay revealed that spermidine decreased the viability of Hela cells in a dose-dependent manner in conjunction with disrupted morphology. Moreover, spermidine caus...
Yasir Sharif1, Saba Irshad1*, Ammara Muazzam1, Muhammad Hamza Tariq1, Ambreen Kanwal1, Sana Rasheed1, Mabel Baxter Dalrymple2 and Anam Tariq1
...1071.9ng/mL, alanine transaminase 117.12±32.001U/L, aspartate transaminase 84.77±18.223U/L and bilirubin 1.02±0.139mg/dL. CBC of control group revealed that all of the studied parameters were normal in them and BTM patients showed significant deviation from control in both hematological and hepatic profile (P < 0.05). Examination for secondary complications revealed that Pallor sign was observed in 79...

Fan Sigang1, Guo Yihui1* and Xu Youhou2

...lated genes, including vasa, nanos, and vitellogenin, and sex-determining genes, such as FoxL2, Dmrt, and sox9, were detected. Transcriptome comparison revealed 2,842 differentially expressed genes (DEGs), of which 591 exhibited biased expression in the ovary and 2,251 exhibited biased expression in the testis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the DEGs were conducted. Results showed that GO terms and ...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
...xtracting was an indispensable process to employ this method. In eDNA extracting of macro-organisms, DNeasy Blood and Tissue Kit was commonly used, but its price was about 80 RMB/sample, which was too expensive. Cheaper eDNA extraction kit was a potential aspect for improvement to expand further application of eDNA analysis. In order to examine some cheaper kits’ effectiveness of extracting eDNA of macro-organisms and ...
Neelam Pery1*, Fatima Ijaz1, Nayab Batool Rizvi1, Munawar Ali Munawar1 and Muhammad Imtiaz Shafiq2*
... failed to produce universal response leaving a large proportion of patients untreated. This is due to complex pathogenesis comprising a network of signaling pathways. By controlling one pathway, the pathogenesis continues through complementary pathways. Janus kinase and bruton’s tyrosine kinase regulates a number of T-cell and B-cell mediated signaling pathways ranging from cytokine expression and antibody production to the bone resorption and cartilage...

Shazia Rasheed1*, Javed Mustaquim2 and Khalil Hasni1

...ties occur almost at the same size which was from 68 to 75 mm short carapace width or 86 to 94 mm long carapace width. Male P. pelagicus matures earlier than the females. The size at which females attains full sexual maturity is 74-86 mm short carapace width (or 93 to 108 mm long carapace width). The size at 50% sexual maturity for male and female crabs was 63.57 mm and 83.55 short carapace width, respectively. The minimum number of eggs was 1, 72, 963 ...
Gautam Patra1*, Ana Sahara2, Sonjoy Kumar Borthakur1, Parthasarathi Behera3, Subhamoy Ghosh1, Apurba Debbarma4 and Seikh Sahanawaz Alam5
...ed from the cages. Blood samples were examined after staining with Giemsa stain. The positive samples were used for amplification of cyt b gene of Plasmodium relictum. Cloning and sequencing of the amplified products of each samples was performed separately. Out of 120 birds examined, 25 were found positive for P. relictum based on m...
Jian Yang1, Guangxiang Tong2, Zhipeng Sun2, Xianhu Zheng2, Weihua Lv2, Dingchen Cao2, Xiaowen Sun2 and Youyi Kuang2*
...posterior part to the dorsal fin at the same developmental stages. In WT and mutants, the density of muscle fiber declined gradually over time. In conclusion, the lack of intermuscular-bones has no influence on adjacent muscle development.
...

Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3

...ys, at three different dosage regimens: 10ρg, 1ηg and 1µg compared to control (saline-treated) rats. Effects on pituitary gonadotropins [Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH)], secretion of testosterone and spermatogenesis were evaluated on alternate day for one spermatogenic cycle. Major techniques applied were; radioimmunoassay, light microscopy an...
Faryal Saad1,3, Zia Ur Rahman2, Aamir Khan3, Rabia Noushin4, Irfan Ullah5, Farman Ullah Dawar3,Shahid Naiz Khan3, Rafiqe Hussain6, Kenza Javed2Abid ur Rehman7, Amna Fayaz3, Zohra Saifullah3 and Kalim Ullah3*
...wa. A total of 188 blood samples were randomly collected from pregnant women of both districts and were examined through Enzyme-Linked Immunosorrbent Assay (ELISA) and Polymerase Chain Reaction (PCR) for CMV. Out of total 88 samples, 73.40% were positive for CMV through ELI
Mushtaq Ahamd Khan1, Ziaul Islam2, *, Amin Ullah Jan3, Kamran Khan2 and Abdullah Shah3
... Linked Immunossorbant Assay (i-ELISA). The study revealed that overall 57.28% sero-prevalence was recorded in women of child bearing age. Highest (56.46%) seroprevalence was recorded in pregnant women as compared to non-pregnant women (43.53%). Highest (57.3%) sero- prevalence was recorded in women having 21-30 years age. Notably, the highest (25%) prevalence was reported in second trimes...

 Amna Arshad Bajwa1, Saher Islam1, Muhmmad Imran1, Karman Ashraf2, Arman Khan1, Muhammad Farhan Khan3, Imran Rashid2, Muhammad Yasir Zahoor1Waseem Ahmad Khan4 and Wasim Shehzad1*

...recise with non-invasive sampling approaches such as faeces, would be valuable. In the present study a set of molecular markers was developed exploiting the AMLx/y gene to assess gender of Punjab urial population in Kala Bagh, using faecal samples as the DNA source. In our study, among 92 urial samples, 54 (58.69%) were identified as female samples, 34 (...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
...tan. A total of 341 milk samples from buffaloes (n=180) and goats (n=161) were screened by (Milk Ring Test) MRT and milk i-ELISA for anti-Brucella antibodies. Higher prevalence was found in buffaloes (5.6% and 16.1%) than in goat (4.97% and 1.9%) both through MRT and i-ELISA respectively. Commercial production a...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...city and repellency bioassays according to completely randomized design. The response of termite workers varied with plant species, botanical concentration and exposure time. The extracts of Azadirachta indica (neem) and Nerium indicum (oleander) appeared to be most effective against the termites with minimum LC50 (6.35 and 10.38%, respectively) and LT50 (12.11 and 17.49 h, respectively) values, followed by the extract of
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...nd filter paper-dip bioassay methods, respectively. Results revealed that the extracts of Mentha longifolia, Sonchus asper and Nerium indicum were the most toxic to D. citri exhibiting 90% mortality. The extracts of Dodonaea viscosa and Olea ferruginea caused highest mortality of S. litura (i.e. 70 and 58%, respectively). Maximum mortality of C. quinquefasciat...
Muhammad Ramzan, Unsar Naeem-Ullah*, Mudssar Ali and Hasan Riaz
...s were present on the dorsal side of female forewings. Female forewings were broader than male wings with dark reddish brown thorax, head and abdomen. Adult hind wings were grayish with reddish brown outer margins. The biological and morphological information of Trilocha varians described in current paper will lead to proper management practices during peak reproductive period of insect to avoid pest outbreak. 
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
... zinc concentration. The sample zinc level is estimated by using the spectrophotometer in laboratory. The level of zinc in cancer patients was observed lower than the optimum value. Male have much lower concentration as compare to females, therefore male are more susceptible to cancer than female. In the nutshell Zinc is an effective anti-inflammatory and antioxidant agent, including as a natural cancer treatment.

...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...wering process share the same precursor. However, this interfering factor could be inhibited by using a high-red LED induction  to delay the flowering stage, which may contribute to higher biomass and glycoside concentrations through greater system productivity. Hitherto, there was no significant study about the effect of far-red LED induction to improve the steviol glycoside content of Stevia plants, particularly at the molecular level. This research was...
Jianjun Fu1, Shili Liu2, 3, Wenbin Zhu1, Yongyi Jia2, 3, Lanmei Wang1, Yufen Yang4, Zhimin Gu2, 3*and Zaijie Dong1*
...s, including twelve microsatellite loci for C. alburnus, and applied parentage analyses using 36 candidate parents and 136 offspring from four independent artificial breeding pilots. Based on allele frequency analysis using 36 candidate parents, the number of alleles ranged from 7 to 22, observed heterozygosity ranged from 0.39 to 0.75, and expected heterozygosity ranged from 0.57 to 0.91. All loci were highly informative (polymorphic information conten...
Xiaopeng Tang1,2* and Kangning Xiong1,2
...4 h for ussing chamber assay. The transepithelial electrical resistance (TEER, Ω.cm2), glutamine and glucose absorption rate (%) were measured. The results showed that: (1) EGF significantly (P < 0.05) increased the TEER of TPEC-J2 cells induced by LPS; (2) EGF significantly (P < 0.05) increased the absorption of glucose and glutamine of IPEC-J2 cells induced by LPS. It can be speculate that EGF repaired the injured cells by reducing ...

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...ng gene (s) required for saponins production is effective in increasing crop yield together with fixing nitrogen. Escherchia coli is still used as a host for the recombinant DNA technology – based industrial production of proteins and peptides to manage root-knot nematode. Mi gene resistance in tomato plants has been utilized to manage M. incognita and M. javanica. Also, protoplast fusion between Pseudomonas fluorescens and P. aerugino

Zubia Rahim1, Gulnaz Parveen1*, Salma Gul2 and Khushnood-ur-Rehman3

Ameliorating Effects of Salt Stress (KCl, NaCl) on Growth and Germination Parameters of Pearl Millet (Pennisetum americanum)
...biotic stresses in which salinity is considered as most pronounced factor to effect the plant growth. This research was performed to assess the effects of NaCl, KCl, a mixture of NaCl + KCl in various concentrations on the growth and germination of Pearl millet (Pennisetum americanum), locally named as Bajra. Five seeds sown in petri dishes to observe the radical, plumule length and germination percentage after treatments to 100, 150, 200 and 250mM concentrati...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation
...ckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gave more shoots (7.33) when the concentration of BAP and IAA was 2.5 and 1 mg L-1, respectively. Alike, Wiallium-8818 hybrid produced the longest shoot ...

Muhammad Abdullah1, Syed Attaullah Shah1, Khurram Nawaz Saddozai1, Jahangir Khan1*, Mohammad Fayaz1, Irfan Ullah1* and Sabeeh Ullah2

Analysis of Agricultural Land Price Determinants and Policy Implications for Controlling Residential and Commercial Encroachments: Facts from District Swabi (Pakistan)
...hwa. For this purpose, a sample of 79 land parcels, having transaction record for the year 2015-2017, was selected from 10 randomly chosen villages. Data were collected on their prices and their physical, agricultural and location characteristics. Descriptive statistical tools were used to summarize data on agricultural land characteristics. A linear Hedonic Pricing Model (HPM) was used to estimate agricultural land prices b...

Muhammad Yasir*, Mansoor ul Hasan, Muhammad Sagheer, Amer Rasul, Rameesha Amjad Ali and Habib ur Rehman

Evaluation of Spinosad Applied to Grain Commodities for the Control of Stored Product Insect Pests
...esidual efficacy of spinosad was assessed by exposing the Oryzeaphilus surinamensis, Tribolium castaneum and Trogoderma granarium to the treated commodities (wheat, maize, rice and oats) at concentrations of 0.25, 0.50 and 1 mg Kg-1 under laboratory conditions maintained at 28 ± 2oC, 65 ± 5% RH and continuous darkness. Seven bioassays were conducted by releasing the insects on treated commodities after differen...

Sajid Khan* and Shahnaz Akhtar

Effect of Farm Labor Transformation on Households Income in Central Districts of Khyber Pakhtunkhwa, Pakistan
... selected through random sampling techniques. The data were collected from 393 sample households and were analyzed by using paired sample t-test through Statistical Packages for Social Sciences (SPSS) and descriptive statistics, percentages, and rank orders. Farm labor transformation was found significantly affecting the earnings of households’ in the study area. Data revealed that m...

Masoud Hassani1* and Omid Madadgar2,3

Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
37th PAKISTAN CONGRESS OF ZOOLOGY (INTERNATIONAL) DEPARTMENT OF ZOOLOGY, GC UNIVERSITY, FAISALABAD, PAKISTAN
...quot;, Helvetica, Arial, sans-serif; margin-bottom: 10px !important;">37th PAKISTAN CONGRESS OF ZOOLOGY (INTERNATIONAL) DEPARTMENT OF ZOOLOGY, GC UNIVERSITY, FAISALABAD, PAKISTAN

Abdul Majid Khan1,*, Muhammad Tahir Waseem1, Rana Manzoor Ahmad2, Ayesha Iqbal1, Hafiza Imrana Naz1, Amtur Rafeh1 and Muhammad Ameen1
..., Chakwal, Pakistan. The sample comprises maxillary and mandibular fragments along with isolated upper and lower teeth. The morphometric analysis of the dental specimens led us to recognize the sample as belonging to Eotragus noyei, which has been considered as the smallest and the oldest bovid in the Siwaliks. Eotragus noyei is characterized by brachydont teeth, finely rugose enamel, more inclined buccal walls...
Yaqiu Liu1, Zhijian Wang 2, Xinhui Li 1*, Jianrong Zhao2 and Weitao Chen 1
...als were returned to the same conditions as the control group for a further 3 d acclimation. The catalase (CAT) and superoxide dismutase (SOD) activity in the gill and gut in P. dabryanus were examined in all three groups after the 7 d stress acclimation, 1 d later, and after a 3 d recovery. It was found that CAT and SOD activity in the gill, foregut, and midgut in the air-exposed group were much lower than in the control group. After recovery for 1 d, ...
Muhammad Mohsin1, Yin Hengbin2,* and Zhang Luyao3
...ndition, it becomes necessary to estimate stock status of fishery resources particularly having commercial importance. Thus, this study is the first time attempt to know whether Spanish mackerel, a major landed fishery resource in Shandong, China, is experiencing overexploitation or not by estimating its stock status. For this purpose, long data series, 2006-2016, comprised of catch and effort figures was statistically analyzed to know maximum sustainable yiel...
Ye Ge1,2,3, Qiucheng Yao1, Hongliang Chai3, Yuping Hua3* and Guohua Deng2*
...species, play an indispensable role in the spread and reassortment of AIVs and provide unknown opportunities for mutation and emergence of novel influenza viruses. Additional studies of AIVs originating from wild birds will help to determine viral adaptation and maintenance in alternative hosts.
...
Jin-Juan Wan, Mei-Fang Shen*, Hui Xue, Hong-Yan Liu, Mei-Qin Zhang, Xi-He Zhu and Chong-Hua Wang

 

...F4 and F5) following the same ration size for 60 days. After the feeding trial, fish were challenged with white spot syndrome virus (WSSV) and cumulative mortality was recorded for the next 4 days. The results showed that the weight gain rate (WGR) of crayfish tended to increase initially and then decrease as feeding frequency increased. The WGR in F3 were significantly higher than those fed 1, 4 and 5 times/day (P<0.05). The highest serum lysozyme (...
Yonghua Liu*, Xianhua Li, Xiongfei Yan and Gang Li
...a (apple), Prunus salicina (plum), and Amygdalus persica (peach) on the growth, development, survival, reproduction, and life table parameters of A. fimbriana were studied. Different host plants had significant effects on the growth, development, and reproduction of A. fimbriana. The overall developmental duration was the longest on apple (60.71 d) and peach (60.78 d) and shortest on apricot (54.55 ± 3.56 d). The cumulati...
Xuya Zhou1, Ying Liu1, Deqin Xu1, Jie Bao1, Yaru Cao2 and Yong Jin3*
...e-linked immunosorbent assay (Elisa) was used to detect the prostaglandin E2 (PGE-2), 20-Hydroxyeicosatetraenoic acid (20-HETE), endothelin 1 (ET-1) and B-type natriuretic peptide (BNP) in blood serum of diabetes complicating arthritis rats. Blood pressure was measured by a noninvasive caudal artery blood pressure meter. Western blot analysis showed that unification of two drugs caused pro...

Muhammad Jamal Nasir*, Atiq Ur Rahman and Anwar Saeed Khan

Assessment of Irrigation Development in the Small Dams Command Area: A Case Study of Palai Dam, District Charsadda
... some 40 km north of Charsadda town. The present study is an attempt to evaluate the impact of the Palai dam on the irrigation system of selected villages of tehsil Tangi. The study is based on an analysis of revenue data acquired from revenue office Tangi, between 2008-09 to 2016-17. The geospatial analysis of collected data is done using the software ArcGIS 10.5.2. The analysis revealed a tremendous increase in canal irrigated area. Before construction of th...

Syed Muhammad1*, Badar Naseem Siddiqui2, Farhat Ullah Khan1 and Nawab Khan3

Self-perceived Constraints of Extension Field Staff Affecting their Working Efficiency in Khyber Pakhtunkhwa, Pakistan
...n in policy decisions, unsatisfactory pay structure, unavailability of medical facilities, low opportunity of proper in-service trainings, poor linkages of agricultural extension with research wing and low social status. The Department of Agricultural Extension, Khyber Pakhtunkhwa, is advised to adopt appropriate measures to address these constraints of EFS in order to enable them to efficiently deliver their services.

...

Ghulam Murtaza1*, Muhammad Ramzan2, Amna Razzaq4, Muhammad Numan3, Rukhsar Beanish4, Ayesha Zafar4, Muhammad Shan Latif3 and Muhammad Adnan3

Screening of Brinjal (Solanum melongena) Varieties against Jassid, Amrasca bigutulla bigutulla
... bigutulla suck the cell sap from underside the leaves resulting in leaves curling and yellowing. Jassid (Amrasca bigutulla bigutulla) inject toxic materials into leaves which results necrosis. An experimental study was conducted by using randomized complete block design (RCBD) during 2018 to check the resistant brinjal varieties (Nirala, Dilnasheen and Bemisal) against jassid (A. bigutulla bigutulla). The highest population...
Adeen Kiran1, Umar Farooq Gohar1*, Ayesha Farooq1, Malik Muhammad Asif2 and Hamid Mukhtar1
Redressal of Antibiotic Resistance using Plant Extracts
...n: justify;">With the passage of time antibiotic resistance is prevailing in microbes and current drugs are not effective enough to cure infectious diseases caused by these resistant pathogens. In this battle, there is a continuous need to develop new drugs or compounds that can cure these infections or to reverse the antibiotic resistance in these pathogens. In recent years different experimental approaches highlighted the importance of phytochemicals present...
Yongmei Gao1, Peihong Gao2, Yanjun Wang3, Jinhua Han4 and Yang Shu1*
... the and overall nursing satisfaction of the research group was significantly higher than that of the control group, p<0.05. It is concluded that combination therapy of mifepristone and Guizhifuling capsule can significantly improve the treatment effect in patients with uterine fibroids.
...

Muhammad Iqbal1, Muhammad Ehetisham ul Haq1*, Muhammad Kamran1, Muhammad Idrees1, Shahid Nazir2, Ihsan Ullah3, Sumera Naz1, Shaukat Ali1 and Muhammad Zafar Iqbal2

Morpho-Molecular Characterization of Xanthomonas Axonopodis Pv. Citri Associated with Kinnow (Mandarin) and its Management
... bacterium from diseased samples was characterized morphologically and molecularly. The relative efficacy of commercially available antibiotics against the pathogen was evaluated in the lab and in field conditions. A systematic survey of randomly ten localities/sampling sites was conducted for a reliable estimation of citrus canker disease in District Sargodha of Punjab Province. Nine samp...
Arshad Mahmood Malik1*, Hafiz Muhammad Tayyab1, Muhammad Arshad Ullah2 and Muhammad Talha Bilal3
Dynamics of Salinity and Land Use in Punjab Province of Pakistan
... is mainly threatened by salinity which is expanding to 6.28 mha, thus developing wasteland of 2 m ha due to presence of highsalinity. The injudicious use of brackish underground water is one of the major factorsresponsible for increased salinity ultimately effecting productivity in Pakistan. Salinity hasdevastating socio- economic impact on farmer’s micro economy in Pakistan, direct...
Anjali Khadka1*, Subodh Raj Pandey2, Subarna Sharma Acharya3, Amrit Poudel4 and Sushma Adhikari1
Morphological Evaluation and Multivariate Analysis of Soybean Glycine max (L.) Merrill Genotypes in Western Mid-Hills of Nepal
...ield. Number of nodes, basal pod height, internodal length, plant height, number of branches, number of grains, pod length at maturity, leaf area was positively correlated with grain yield while days to 50% flowering, days to maturity, number of trifoliate, number of pods and fresh weight of the pod were negatively correlated with grain yield. The path analysis depicts that the internodal length and days to maturity had the highest direct positive and negative...

Ahmed Weli Hussein Osman1,2, Rakibul Islam1, Md. Bazlar Rashid1, Abdiaziz Idiris Mohamud2,3* and Pravin Mishra4

Nanoparticle Tactics for the Oral Delivery of Insulin in Alloxan-Induced Hyperglycemia in Rat (Rattus norvegicus domestica) Model
...n with nano particles of same dose as insulin. Over the sequence of the experimental trials, observations were noted for blood glucose level, glycosylated hemoglobin, and body weight. Blood glucose levels were increased significantly (P<0.05) in all treated groups compared to the negative control group. Similarly, bodyweight was decreased significantly (P<0.05) in all alloxan treated groups compared to negative control. There was significant falling in b...

 Shafii Abdullahi Mohamed1, Abdiaziz Idiris Mohamud2*, Yonis Abukar Mohamed3, Pravin Mishra4 and Osman Sheikh Ali Jama5

Assessment of Knowledge, Attitude, and Practices of Population Towards Brucellosis in Benadir Region, Somalia
...ional study using random sampling was conducted from May to September 2020 in Benadir region of Somalia, to determine the knowledge, attitude, and practices of population towards Brucellosis. In this study, a total of 120 participants share their knowledge, attitude, and practices towards Brucellosis. Their knowledge regarding causative agent was 37.5% (n=45) and the disease was 45% (n=54), while transmission of animal to hu...
Abdul Hameed1*, Ihtsham ul Haq Padda2 and Abdul Salam3
Estimating Food Consumption Patterns in Pakistan by Using Ideal Demand System
...y price subsidization or safety nets programmes for deprived groups. In addition, the state should concentrate on rural income generation activities such as agriculture, livestock and its related industries.

...

Muhammad Ali Shah, Faiz Ur Rehman, Abbas Mehmood, Fareed Ullah, Sayed Irfan Shah and Syed Majid Rasheed*

Combining Ability, Heritability and Gene Action Assessment in Rapeseed (Brassica napus L.) for Yield and Yield Attributes
...esearch Farm (BARF), Charsadda, Khyber Pakhtunkhwa, Pakistan during the growing season of 2017-18. The data were collected for pods related traits and total yield. The results of the study showed significant differences among parents and F1’s for all the studied traits. General and specific combining ability analysis revealed non-significant results for the traits under consideration, suggesting the involvement of non-additive type of gene action. A high...

Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz

Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar
...on. A total of 100 fecal samples  were collected aseptically from four different zones of district Peshawar during the period of February, 2019 to April, 2019. C. perfringens  Type D was confirmed through bacterial culturing, Gram staining, biochemical tests and polymerase chain reaction (PCR) . Results revealed that 25 feacal samples collected from suspected goats were positive for C. perfringens  Type D ...

Ghulam Rabbani1, Uzma Javed1*, Javed Iqbal2, Ruqeah Mustafa3, Ghulam Shabbir2 and Fida Hassan Shah4

Barani Mash a Newly Developed Disease Resistant and High Yielding Mash Cultivar for Rainfed Areas of Punjab, Pakistan
...s resistance to yellow mosaic. The main yield contributing characters were number of pods per plant, number of primary branches per plant, yield per plant and 100-kernal weight. Based on desirable traits and higher yield, this line was approved with name of “Barani Mash” by the Punjab Seed Council for its cultivation on commercial level in rainfed areas of Punjab. “Barani Mash” will prove to be a good alternative of existing cultivars a...

Muhammad Shahzad Akbar, Farrukh Sajjad, Muhammad Afzal, Muhammad Luqman, Muhammad Asam Riaz and Muhammad Zeeshan Majeed*

Field Evaluation of Promising Botanical Extracts, Plant Essential Oils and Differential Chemistry Insecticides against Subterranean Termites Odontotermes obesus (Isoptera: Termitidae)
... extracts (Dodonaea viscosa, Gardenia jasminoides and Nerium indicum), plant essential oils (Allium sativum, Citrus aurantium and Cymbopogon citratus) and differential-chemistry insecticides (chlorantraniliprole, chlorfenapyr, emamectin, indoxacarb, pyriproxyfen and triflumuron) against subterranean termites (Odontotermes obesus) attacking sugarcane crop. Setts of sugarcane variety HSF555 were sown on the ridges under RCB de...

Muhammad Luqman1*, Roshan Hussain1, Muhammad Yaseen1, Muhammad Umer Mehmood1, Ijaz Asghar2 and Usman Saleem1

Comparative Analysis of Dietary Intake Patterns of Rural and Urban Communities of Southern Punjab, Pakistan
...Southern Punjab. Exactly sample size of 600 respondents (300 rural respondents and 300 urban respondents) was randomly selected through equal distribution technique and interviewed by using structured interview schedule. Data were analyzed using Statistical Package for Social Sciences and the following key results were obtained. Most of the respondents from the study were above the 30 years of age (mature), illiteracy was high in rural area and most of the res...

Amjed Ali1, Rafi Qamar1*, Muhammad Ehsan Safdar1, Salman Saleem2, Sami Ullah3, Muhammad Arshad Javed4 and Syed Wasim Hasan5

Development and Growth: Influence of Sowing Dates on Performance of Cotton Cultivars

Ali Zohaib1*, Habib Ullah2, Shakeel Ahmad Anjum2, Tahira Tabassum2, Muzzammil Hussain1, Mohsin Nawaz3, Ghulam Abbas4 and Sohail Irshad5

Effect of NPK Fertilization Strategies on Growth, Yield, Nutrient Use Efficiency and Economic Benefits of Relay Intercropped Wheat in Cotton
...o (BCR) were obtained by same NPK application timing (i.e. T4). In conclusion, fertilization timing substantially affected the yield, NUEA and economic benefits of relay intercropped wheat in cotton; and application of ½ N and all PK at sowing and ½ N at tillering was most appropriate option.

...

Imran Ahmad Rana*, Muhammad Ali Yab and Ch. Abdul Rehman

Quality Engineering Tools Implementation a Comparison of Indian and Pakistani Business Organizations
...ma practices on customer satisfaction, where common elements like SS Training management, Information and Communication planning and execution, and quality control practices found statistically significant variables explaining variability of customer satisfaction. The study may help leaders, engineers, professionals working at Indian-Pakistan based organizations to know significant organizational behaviors associated with su...

Mehreen Ijaz

Effect of Weave Type on Tensile Strength of Cotton Fabrics
...such as plain, twill and satin. Tensile strength of each prepared fabric was determined by following Strip Methods ISO 13934-1. The results depicted that plain weave structure had better tensile strength both in warp and weft directions followed by twill and satin weave. It was due to the less interlacing per unit area of the prepared fabric. Moreover, plain weave helped to create more compact structure that resisted the pre...
Jun Yan Bai1*, Ren Tao Di Wu2, Qiang Zhang2, Di Bao2, Le Ma Dao2 and Xing Hua Tian3
...">In this study, 17 microsatellite markers were used to analyze the genetic diversity of Sonid bactrian camels, and the correlation between microsatellite markers and their body size was analyzed to find microsatellite markers associated with the body size traits of Sonid bactrian camels, so as to provide reference for marker-assisted selection of Sonid bactrian camels. The results showed ...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...ulcers of intestinal mucosa, hemorrhages in trachea and spleen. Histopathological lesions observed in proventriculus were erosions in epithelium, localized hemorrhages and congestion in the glandular region. In small intestine of affected birds, villi appeared hyperemic and ulcerated. Tracheal surface epithelium often showed discontinuation, the loss of cilia of the lining epithelium and disturbance of the coherence among various histological layers. In spleen...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...ant and dominant models; same hold true for the Asian population. Stratification by ethnicity revealed that in Asian population also, the association with RA manifestation is significant. However, in a cohort of 200 RA and 176 healthy subjects (n=376) from Pakistan, significant association between IL-6 -572 G/C polymorphism and progressive RA could not be established despite the fact that GG genotype exhibited higher susceptibility (p=0.088) towards RA in comp...
Zhilong Tian1, 2, Yuqin Wang2, Jishun Tang1,3 and Mingxing Chu1*
...ail han sheep (STH) were sampled to detect single nucleotide polymorphism (SNP), and Sequenom Mass ARRAY®SNP assay was applied to genotype SNP loci of the SYNE2 gene. In this study, four SNPs were identified and that SNPs were identified that involved in amino acid changes. Population genetic analysis indicated that SYNE2 gene g.73310578G>A, g.73312791A>G showed moderate polymorphism (0.25&...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
Saima Yaqub1, Tahir Yaqub1*, Muhammad Zubair Shabbir2, Asif Nadeem3Aziz-Ul-Rahman1, Muhammad Furqan Shahid1, Zarfishan Tahir4 and Nadia Mukhtar4
...evise and implement necessary interventions to control disease in developing countries like Pakistan. A total of 201 plasma samples were collected from IDUs. Samples were first screened using HIV-1 Ag/Ab Combo test and then antigen positive samples were amplified for HIV-1 pol gene (1084 bp) and analyzed. Initial screening showed a total of 49 HIV sample...
Chaojie Yang1,2,3, Haishan Wang1,2,3, Le Ye1,2,3 and Zhi Chen1,2,3,*
...collected Muraenesocidae samples in our present study. Teeth characters that are conclusive for the species were consistent with Muraenesox and were sufficient for separation from other Muraenesocidae species. The morphological results further suggested that only two Muraenesocidae species belonging to Muraenesox inhabit the East China Sea.This study confirms the occurrence of Muraenesox bagio (Hamilton, 1822) misreported as Congresox talabonoides

Zohaib Ahmad Hassan1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Usman Saleem3, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz4, Khurshid Ahmad Mufti5 and Aamer Sattar2

Toxic Effects of Sodium Bicarbonate (NaHCO3) on Growth and Yield of Rice
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5Hamayun Khan1 and Shabana Naz6*

 

...low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality...

Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu* 

...0 mg / kg, p.o.). Intranasal OVA has been instilled on the 21 days. Biochemical parameters, spleen weight, physiological parameters, interleukin (IL-1β and IL-6) parameters and immunoglobin-E (IgE) were calculated at the end of the experimental study. To investigate the potential mechanism of action, Paw edema and mast cell de-granulation are estimated. Used to measure immune and inflammatory mediators, qRT-PCR technique. Quercetin significantly (P<0.0...
Muhammad Riaz1*, Zahida Tasawar1, Muhammad Zaka Ullah2 and Zawar Hussain3
Parasitological and Molecular Survey on Theileriosis of Sheep and Goats and the Related Risk Factors in Musa Pak Shaheed Town, Multan, Pakistan
... PCR and microscopy in Musa Pak Shaheed, Multan district, Pakistan amidst 2013. A total of 200 small ruminants (93 sheep and 107 goats) without any apparent signs of theileriosis were examined to diagnose Theileria piroplasms. During sampling, blood was amassed by puncturing the jugular vein in eppendorf comprising EDTA as anticoagulant coated tubes for the detection of Theileria piroplasms. DNA was extracted from collected ...

Said Sajjad Ali Shah1*, Muhammad Ilyas Khan1, Aziz Ullah1, Hayat Ullah2 and Faisal Ahmad2

Coprological Examination of Small and Large Ruminants in Central Zone of Khyber Pakhtunkhwa
...e, a total of 1700 fecal samples were received from central districts and processed for coprological examination. Out of 1700 samples, 947 samples (55.7%) were positive for intestinal parasites. Specie wise prevalence of GI parasites was recorded as 51.5% in cows, 58.3% in buffalo, 73.9 % in sheep and 68.5% in goats. Trichostrongylus (32.8%) was recorded as highly prevalent in cattle follo...

Zarqa Khalid1*, Muhammad Asad ur Rehman Naseer1, Raza Ullah1 and Shahzad Khan2

Measuring the Global Trade Competitiveness of Pakistan’s Cotton Crop
... cotton while having a disadvantage in imports of cotton and also has no specialization in its exports. The results suggested that Pakistan has to explore new potential markets to strengthen its comparative and competitive advantage as well as raise cotton export earnings.

...

Sanaullah1*, Abdul Basit2 and Inayat Ullah3

Challenges and Prospects of Farm Mechanization in Pakistan: A Case Study of Rural Farmers in District Peshawar Khyber Pakhtunkhwa
...y employed a multi stage sampling technique to gather data on the socio-economic features of the farmers and available machinery. A total of 240 rural farmers were randomly selected from two local union councils of provincial government Khyber Pakhtunkhwa Peshawar. The accumulated data were analysed using mean and standard deviation with an acceptance mean value of ≥3.00 and estimating logit model. Socio demographic features revealed that majority (52%) wer...
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...r. Freshly dropped fecal sample were collected once before treatment with anthelminthic drug and twice after treatment and brought to laboratory for qualitative and quantitative analysis. Direct smear method and fecal floatation technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites were i...
Hongqun Li1*, Xiaoli Liu2*, Zongxian Han1, Shabin Wu1 and Changlei Cao1
...n of the brown-eared pheasant (Crossoptilon mantchuricum) was studied in Huanglong MountainsNature Reserve, Shaanxi Province, China from October to November 2018. Data were collected by examination of fresh sites used by the brown-eared pheasant located on 22 transects across the entire study area. The results showed that foraging habitats were characterized by broadleaved forest, mid and upper slope location, and gul...

Zaka ur Rehman1*, Kashif Akbar1, Altaf ur Rehman1, Hassan Waqas1, Alif Khan1 and Asad Ali2

Development and Storage Stability of Diet Mango Juice
...e) instead of sugar. The samples were filled and sealed in 300 ml of transparent glass bottles. Then diet mango juice samples were stored at room temperature for physicochemical analysis (Total soluble solids, ascorbic acid, pH, percent acidity, and sugar-acid ratio) and organoleptic evaluation (appearance, taste, flavor, overall acceptability) for three months. These were prepared with sweeteners, numbered as (T0) pure mang...

Shawaiz Iqbal1*, Nadeem Iqbal2, Usama Bin Khalid1, Muhammad Usman Saleem1, Adila Iram1, Muhammad Rizwan1, Muhammad Sabar1 and Tahir Hussain Awan1

Growth, Yield and Economic Analysis of Dry-Seeded Basmati Rice
... a contemporary resource saving crop establishing technique, which enables farmers to harvest economical rice production. Field study was conducted at Rice Research Institute, Kala Shah Kaku, Pakistan in 2014 and in 2015 to evaluate the different planting methods. Three dry-seeded rice (DSR) planting methods viz. (i) DSR-broadcast, (ii) DSR-drill, (iii) DSR-ridges were compared with the conventional transplanting (TR) method in lines having row to row and plan...

Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3 and Waqas Raza1*

Influence of Resistance Inducers on Nitrogen, Phosphorus and Potassium Contents of Susceptible Chickpea Cultivars after Inoculation with Ascochyta rabiei
... (acibenzolar-S-methyl), salicylic acid (SA), potassium hydroxide (KOH) or plant extracts of neem (A. indica A. Juss.), datura (D. metel L.) and garlic (A. sativum L.) were investigated 7 and 14 days after inoculation with Ascochyta rabiei. Elevated nitrogen (2.95%), potassium (1.05%) and potassium (533.00 ppm) contents in the tissues decreased disease s...

Delower Hossain1,5, Shuvo Singha2,5, Chelsea Marie Van Thof3, Sanna Gough3 and AHM Musleh Uddin4,5*

Effect of Somatic Cells on Milk Quality and Human Health
...l strategies. Again, the safety margin evaluated though SCs ensures safe and healthy milk at consumer level devoid of bacterial presence in milk. This review highlights a brief overview of the importance and demand of estimating somatic cell count (SCC) and its effects on milk quality and human health.

...

Ambreen Akhtar Saddozai1, Amer Mumtaz2, Naseem Rauf1, Saeeda Raza2, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Sahar Shibli2, Muhammad Suhail Ibrahim3*, Muhammad Akhtar4 and Muhammad Saad Rehan5

Preparation and Quality Evaluation of Soymilk Carrot Blend
...g the storage period the samples were analyzed for different chemical parameters total soluble solids, pH, total carotenoids, protein contents and antimicrobial analysis. On the basis of quality attributes it was observed that plain and carrot supplemented soymilks remained acceptable up to 7 days at refrigerated temperature. Plain soymilk had lower TPC at both temperatures than carrot supplemented milk. pH, TSS, carotenoids and protein contents in

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Mushtaq Ahmad1, Muhammad Saleem2, Ali Aziz2, Irfan Rasool2, Zeshan Ali3 and Muhammad Amin2

An Update on Biology, Extent of Damage and Effective Management Strategies of Chickpea Pod Borer (Helicoverpa armigera)
...ize, sunflower, sorghum, safflower, pigeon pea, okra and tomato), installing animated bird perches and application of biological control measures (use of plant extracts, virus/bacteria based insecticides). In case of pod borer outbreaks, chemical insecticides remain as last option for farmers. However, management of chickpea pod borer through use of resistant cultivars, adopting recommended cultural practices and biological control measures have been found mor...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Sher Muhammad3, Usman Saleem4, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz5 and Aamer Sattar2

Impact of NaCl Toxicity on Yield and Yield Components of Rice (Oryza sativa) Grown in Aridisol
...">Measurement of soluble salt content in soil is termed as soil salinity. Both positive and negatively charged ions are contained in such soils and being free can be absorbed by plants. A pot study was performed to assess the poisonous impact of NaCl salinity on rice growth parameters. Selection of soil with customary characteristics was done. Various levels of sa<...

Zaheer Ahmed Deho*, Saifullah Abro and Muhammad Rizwan

Assessment of Stability for Seed Cotton Yield of Cotton Genotypes Across Different Environmental Conditions of Sindh Province
Fangqing Liu1, Jared Atlas2, Chaohao Du1, Anoop Das3 and Longying Wen1*
...ing-necked or common pheasant Phasianus colchicus across mainland China in response to environmental factors. We collected 399 pheasant and divided them into two groups: high altitude (>1500m) and low altitude (<1500m). The results that all morphological size measures of males were significantly higher than those of females (P<0.05). Most measures of size at low altitude significantly greater than thos...
Ahmad Sadiq1, Muzafar Shah2*, Habib Ullah1, Irfan Ali1, Amir Alam3
Navid Jalil1 and Muhammad Khan3
...oprotein (HDL) was found same in both male and female. Moreover, patients with HbA1c > 7 have 26.66% stroke, 4.44% angina, 2.22% ischemic heart disease and 27.77% micro vascular complications.
...
Nadia Islam1, Khalil Ahmed1,*, Maisoor Ahmed Nafees1, Mujtaba Khalil2, Ishtiaq Hussain3, Muhammad Ali1 and Raja Imran1
...s. A total of twenty one samples were collected and analyzed by membrane filtration method. At Misto uch, the mean temperature was 9.7°C and 15.4°C, turbidity was 0.44 NTU and 0.67 NTU, electric conductivity was 147.4 µS/cm and 226.7 µS/cm, total dissolved solids was 99 mg/l and 118 mg/l, pH was 6.8 and 6.8 and total phosphorus was 48.3 µgP/L and 64.3 µgP/L in both the seasons. Whereas Bar spring the mean values in both the...
Zhen-Yang Wu1, Li Li1, Yu-Hua Fu2, Sheng Wang3, Qing-Ming An1, Xiao-Hui Tang4, Xiao-Yong Du3,5,* and Fei Zhou6,*
...nced genes from six skin samples using Solexa sequencing. The RNA-Seq analysis generated 63,283,784 and 63,644,062 clean reads in black and white skin, respectively. A total of 60 differentially expressed genes (DEGs) were identified, providing evidence that the different coat color skin changed considerably. Among these differentially expressed genes, there were 18 up-regulated genes and 42 down-regulated genes in black skin tissue. Further analysis showed th...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...% AES and 10% ABS. At passage 4, each group showed same morphology and appearance, there was also no significant difference between any groups, when viability was compared by using Trypan blue staining. Collagen1 was also equally expressed in all groups and there was no significant difference observed. Thus, present study concluded that AES or ABS could also be used as affordable alternatives to FBS. ABS could be preferred a...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...alaysian zoo. Ten faecal samples were collected from pumas (Puma concolor, n = 5), African lions (Panthera leo, n = 3), a spotted leopard (Panthera pardus, n = 1), and a black panther (Panthera onca, n = 1). All faecal samples were examined for parasite eggs, larvae, and oocysts by simple faecal floatation and formalin – ether sedimentation technique. All large felines in the zoo were infect...
Jun Yan Bai*, Kun Peng Shi, Xiao Ning Lu, Xiao Hong Wu, Xue Yan Fu, Heng Cao, Hong Deng Fan, Meng Ke Chen and Yong Kang Ma
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
...nes, respectively. Serum samples were collected on 21, 28 and 35 days post vaccination for anti-MG-ELISA antibody titer. Cumulative mean anti-MG-ELISA antibody titer of birds to G-MG, O-MG, G-MG+NDV, O-MG+NDV and control was 80.30, 137.49, 91.59, 169.76 and 0.15 units respectively on 35 days post-vaccination. It is con...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...sexual maturity was necessary to optimize reproductive performance. In fertility trial, a significantly higher embryonic mortality (8.65%) and significantly lower hatchability (90.48%) was found in males fed with 135.5g/d diet than other two groups (p<0.05). Taken together, feed allocation of 125.5 g/d under ME 2770 kcal/kg and CP 15.4% was recommended to broiler breeder males of genetically modified F line after sexual maturity. 
...
Oluwatobi Emmanuel Olaniyi1,2*, Chukwudiemeka Onwuka Martins2, Mohamed Zakaria2
...r Malaysia. The distance sampling point count technique using stratified random design was employed to survey (from November 2016 to December 2018) and choose 57 and 54 point stations around 14 and 24 lakes of PIW and PW respectively. Significant differences (p<0.05) existed in the encounter rate and effective detection radius of PPI and AP between PIW and PW. Both wetlands had low site occupancy, an unevenly distributed and non-significantly relative abund...
Sana Mehmood1, Hafiz Muhammad Tahir1*, Muhammad Summer1, Sher Muhammad Sherawat2, Shaukat Ali1*, Sajida Naseem3
...rcode analysis of tissue samples. A standard barcode sequence of COI (Cytochrome c Oxidase I) was recovered from 90 specimens of spiders. Percentage accuracy of morphological identification was 92.10%. Four morphologically misidentified spiders were allotted to correct taxon after molecular identification. To describe the species diversity and richness in numerical structure, diversity indices (Shannon’s and Simpson’s) and evenness indices (Margalf...

Muhammad Shahid1*, Unsar Naeem-Ullah1, Waheed S. Khan2, Shafqat Saeed1 and Kashif Razzaq3

Application of Nanotechnology for Insect Pests Management: A Review
...he designing of greener, safe and environmentally benign protocols for the synthesis of nanoparticles. Insect pests are main density dependent factors that deteriorate the quality and production of various crops i.e. vegetables, fruits, ornamental and field crops. In past decade, these insect pests had been controlled by the application of synthetic insecticides but due to the injurious application of these insecticides causes the development of resistance, en...

Inam Ullah1,2, WU Qing-Ming1*, Ruqia Bibi2 and Najam Un Nisa2

Comparison of Nesting Site Preferences and Breeding Ecology of Red-Vented Bulbul, Baya Weaver, and Grey Bush Chat in Sheikh Badin National Park Dera Ismail Khan, Pakistan
...tudy period at different sampling sites. All the three birds’ nest were observed on Acacia Nilotica. The average highest nests   height of 5.06 ± 0.43 m were observed for Red-vented bulbul. Maximum Nest n=8 of baya weaver bird observed. Grey Bush Chat successfully hatched 100%.   The average incubation days for Red-vented Bulbul l12.83 ± 1.16, Baya weaver 12.71 ± 0.75, and Grey bush chat 13.25 ± 1.5 were re...

Rahat Afza, Muhammad Asam Riaz*, Muhammad Afzal and Muhammad Zeeshan Majeed

Adverse Effect of Sublethal Concentrations of Insecticides on the Biological Parameters and Functional Response of Predatory Beetle Coccinella septempunctata (Coleoptera: Coccinellidae) of Brassica Aphid
...e insecticides was found safe and all insecticides have had adverse effects on the life parameters of coccinellid beetle C. septempunctata. However, thiamethoxam, lambda-cyhalothrin and cypermethrin did not change the functional response of larval coccinellids; while, these insecticides increased the handling time and decreased the maximum attack rate relative to control. Thiamethoxam, lambda-cyhalothrin and cypermethrin were considered relatively

Sabina Noor1*, Fatimah Abang1 and Hamady Dieng2

Biology of an Exotic Butterfly Acraea terpsicore (Linnaeus, 1758) (Nymphalidae: Heliconiinae), in a Newly Invaded Region, Sarawak, Borneo
Luka Anthony*, Olugbenga Omotayo Alabi, Elizabeth Samuel Ebukiba and Vandi Gamba
... households. Multi-stage sampling technique was adopted. Data were analyzed using Multinomial Logit Model, Gini-Coefficient, Double-Log Regression Model, (Cobb-Douglas) and Principal Component Analysis. The results of the Multinomial Logit regression model show that among the significant factors that influence the choice of rice market outlets were: sex of the household head (P<0.10), educational level (P<0.10), access to credit facilities (P<0.05), e...
Alam Zeb* and Amanullah Jan
...eld in oil seed crops. Sesame is being considered the most tolerant crop for avoiding drought conditions with higher yield. Experiments on “response of sesame to sowing methods, nitrogen and sulphur levels” were carried out in Agronomy Research Farms, The University of Agriculture, Peshawar. During two consecutive Kharif growing seasons of 2013 and 2014, respectively. Three sowing methods (flat, ridges ...
Vijay Lal and Muhammad Naeem*
... of T. jarbua. 70 samples of T. jarbua ranging from 18.00-29.90 cm in length and 119.60-429.50 g in body weight were collected from Fisheries Harbour, Karachi Pakistan. The mean percentage of constituents were found to be for water 73.22%, ash 6.72%, fat 3.45% and protein 16.62% in the whole wet weight of body of T. jarbua. % water represented correlation with highly significant value (P<0.001) with protein, ash, fat and organic constit...

Tofique Ahmed Bhutto1, Mahmooda Buriro1, Niaz Ahmed Wahocho2, Safdar Ali Wahocho2, Muhammad Iqbal Jakhro3*, Zulfiqar Ali Abbasi1, Reema Vistro1, Fehmeeda Abbasi1, Sanam Kumbhar1, Fateh Muhammad Shawani4 and Nadar Hussain Khokhar5

Evaluation of Wheat Cultivars for Growth and Yield Traits under Agro-Ecological Condition of Tandojam
...of crop cultivars of the same species for growth and yield-related attributes is well documented. The present investigation investigated the performance of wheat cultivars under Tando Jam conditions. For this study, two-year field experiments were performed during October 2015-2017 (both seasons) applying randomized complete design with three replications. Performance of various wheat varieties for their yield and yield-related characters, including TD-1, Moom...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz3, Abid Niaz2, Khurshid Ahmad Mufti4 and Muhammad Arif2

Deleterious Impact of Sodium Chloride (NaCl) Toxicity on Chemical Properties of Soil and Ionic Composition of Rice
...leterious impact of NaCl salinity on rice growth parameters. Selection of soil with customary characteristics was done. Various levels of salinity; < 4, 4, 6, 8, 10, 12 and 14 dS m-1 were established precisely using NaCl salt. Soil was left for appropriate period (30 days) for the accomplishment of salinity. Completely Randomized Design (CRD) was used...

Saba Aleem1*, Mehvish Tahir2, Iram Sharif3, Muqadas Aleem4, Muhammad Najeebullah2, Ali Nawaz1, Amina Batool1, Muhammad Imran Khan1 and Waheed Arshad1

Principal Component and Cluster Analyses as Tools in the Assessment of Genetic Diversity for Late Season Cauliflower Genotypes
... germplasm is an indispensable assist for crop improvement strategies. Multivariate analyses such as cluster and principal component analysis measures the amount of genetic diversity in respect of several charaters and asseses the relative contribution of different traits to the total variation. In the present study, nineteen genotypes were evaluated for presence of genetic diversity through principal component and cluster analyses. The experiment was designed...

Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review
... infrastructure and necessary facilities. Therefore, authors planned to document different critical stages of olive micropropagation like; media preparation, culture establishment, shoot and root proliferation and acclimatization which would be applied for micropropagation of olives at Barani Agricultural Research Institute (BARI), Chakwal. Resultantly, olive expansion through micropropagation can be predicted in near future for sustainability of olive sector ...

Hafiz Hammad Ahmad1, Mukkram Ali Tahir1*, Noor-us-Sabah1, Ghulam Sarwar1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Aneela Riaz4

Growth and Yield Response of Sunflower to Organic Amendments in Aridisol

Muhammad Farooq1*, Elham Azadfar2, Zohre Bahrami3, Mahniya Sharifi3, A. Shakoor4, Shabir Ahmed5, Iftikhar Ahmed Solangi1, Muhammad Noman Khan6, Memona Siddique7, Naila Ilyas8*, Muhammad Bakhtiar9, Mohamed El-Fatieh Ismael1 and Wang Yunyang1

Optimization of Drying Process of Rambutan by Combined Method of Osmosis Ultrasound and Complementary Drying of Hot Air
...er osmosis pretreatment, samples were put under an osmosis dehydration process. First, using Osmosis-ultrasound with 40.5% concentration in Sekanjabin solution then dried in an oven at a temperature of 70°C. The characteristics of solid gain, water and weight loss, shrinkage and rehydration were evaluated. The obtained results showed that by increasing the concentration of weight and water loss, solid gain increased. Accordingly, in a higher concentrated o...

Hillary M.O. Otieno

A Review of White Mango Scale (Aulacaspis tubercularis Newstead; Hemiptera: Diaspididae) in Sub-Saharan Africa: Distribution, Impact and Management Strategies
Muhammad Umar1, Muhammad Arshad2, Mubashar Hussain1*, Moazama Batool3 and Muhammad Faheem Malik1
...+ were higher than safe limits. The concentration of these metals showed variation in different months of the year. An extensive monitoring of the lake and catchment area is suggested in order to address the threats posed by heavy metals.
...
Ashara Sajid1, Muhammad Usman Ghazanfar1, Saeed Rauf 2, Zahoor Hussain3, Salman Ahmad1 and Yasir Iftikhar1,*
...culate Blanco) and Mosambi (Citrus sinensis (L.) Osbeck), among significant citrus-growing areas of Sargodha district based on symptoms. Random samples of trees within orchards were scored based on characteristic symptoms like blotchy mottle, lopsided unripe fruits, and aborted seeds were observed. The highest incidence of 26% was recorded in Kot Momin, while the lowest incidence of ...
Umair Faheem1*, Qaisar Abbas1, Saghir Ahmad2, Ghayour Ahmad2, Abdul Karim2and Mussurat Hussain1
...t is attacked by several sap sucking and chewing insect pests during its growth, which results in the significant reduction of the cotton yield. Among these insect pests, Amrasca biguttula biguttula ‘Jassid’ are the most destructive ones. Beside these insect pests, environmental factors also affect the cotton production as well as population fluctuations of insect pests. The current study was designed to measure the impact of different meteo...
Faiza Altaf1, Shamim Gul1,2*, Tasawar Ali Chandio3, Gul Bano Rehman1, Attiq-ur-Rehman Kakar4, Sami Ullah4, Naqeebullah Khan4, Umbreen Shaheen5, Muhammad Naeem Shahwani6, Muhammad Ajmal7 and Misbah Manzoor8
 
 
...lettuce was grown in the same soil. As compared to control, amendments of biochar-poultry manure mixtures had a significantly positive influence on growth of both crops. There was ~ 254.49% - 871.9% (~3.5 – 9 fold) increase in aboveground biomass of tomato and 25.67% – 94.59% increase in aboveground plant biomass of lettuce. These fertilizers also increased significantly water use efficiency by ~3.5 – 12.5 times (364.8% - 1166.6%) of tomato p...
Abdul Rauf Bhatti1*, Ahmed Zia1, Falak Naz2, Amjad Usman3, Rukhshanda Saleem4, Ghulam Sarwar5 and Amad Ud Din6
 
 
...ecords viz. Ptochus assamensis, P. ovulum and P. limbatus. The species were recorded from walnut (Juglans regia) trees and Khabbal Grass (Cyanodon dactylon), which highlight their importance. Keeping in view topographic gradients, ecological complex in Pothohar plateau and pest status of curculionid weevils, further surveys are suggested to unveil more important records from the area.
...
Imran Haider1, Muhammad Aown Sammar Raza1*, Rashid Iqbal1, Salman Ahmad1,2, Muhammad Usman Aslam1, Muhammad Israr3, Umair Riaz4, Maliha Sarfraz5, Nasir Abbas6, Salman Haidar Abbasi7, Zafar Abbas8 and Muhammad Aamer9
 
 
...antly mitigated the adversarial effect of drought on the wheat as compared to the treatments in which drought was imposed without the application of yeast and amino acid. However, yeast more effectively moderated drought effects than amino acids. Maximum spike length (15.73 cm), spike number (18.75), grains per spike number (54.50), 1000 grain weight (44.08g) seed yield (5.445 tons), sugars, proline, total phenols, amino acids, and carbohydrates were recorded ...
Nasir Rashid1,2*, Javaid Iqbal1,2, Umar Shahbaz Khan1,2, Mohsin Islam Tiwana1,2 and Amir Hamza1,2
...evious studies using the same dataset for calculating multi-class classification accuracy. 
...
Nowsher Yousaf1*, Bushra Khan1, Dennis Flanagan2 and Ishaq Ahmad Mian3
...ecting soil erosion. The sandy clay loam soils of dense forest lands had a relatively small intensity of erosion (2.1 mm.yr-1) due to mean annual rainfall of 31.46 mm.yr-1 with high soil infiltration capacity value of 19.8 cm/h while the sandy clay loam soils of moderate forest lands having soil infiltration capacity value of 16.4 cm/h have mean annual erosion of 2.9 mm.yr-1due to mean annual...
Abdul Hafeez1*, Akhtar Nawaz Khan2 and Zahid Ullah3
... insufficiently rigorous sampling rate. Furthermore, the phases leading to useful decision-making often follows pre- and post-processing in a subjective manner, which is tedious, time-consuming and erroneous. An important and an unrealized need of these systems is the real-time answer from the acquired data, which is often multi-dimensional and at remarkably high-resolution. The inherit noise in the data is another major bottleneck for real-time processing. Th...
Tariq Mahmood Khalil1,2*, Muhammad Ajmal3, Iftikhar Zeb1,4 and Muhammad Azeem Khan1,5
 
 
...ping countries are using saline water for irrigation. This laboratory scale study was aimed to investigate the effects of saline water irrigation on development of total water potential within coarse and fine textured soils using a novel method based on water potential dynamics. Chilled mirror dew point hygrometer accurately captured the water potential dynamics when soil samples were irri...
Jia Shi
...patitis patients is necessary as they are vulnerable to developing cirrhosis and hepatocellular carcinoma. The present study was aimed to evaluate the response rate of treatment by Peginterferon alfa-2a. In this systematic review and meta-analysis, a review of articles evaluating response rate to Peginterferon alfa-2a was done through the databases of Scopus, Google Scholar, Pubmed (inception - 2019). Fourteen studies reporting the response rate of HBeAg negat...
Ghazanfar Ali and Riffat-un-Nisa Awan*
... between CTIPs and AA. A sample of 320 final year science students of BS programs, was selected from three selected universities and surveyed through a questionnaire. The results of data analyses revealed gender wise differences in perceived level of CTIPs, CTS and AA in favor of female students. Students of physics were found better in CTS and students of chemistry were found better in academics. Teachers of chemistry were employing CTIPs more frequently than...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...this problem, it is necessary to explore alternate methods for production of non-conventional proteins utilizing the available cheap sources. The indigenous Saccharomyces cerevisiae were isolated from different fruit samples, identified by conventional polymerase chain reaction (PCR) and were characterized for production of single cell microbial protein (SCMP). Out of 60 different fruit sa
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
...">Pseudomonas aeruginosa is an opportunistic pathogen and the leading cause of nosocomial infection worldwide. This bacterium produces enzymes known as extended spectrum β-lactamases which render broad spectrum cephalosporins and penicillins inactive. This study reports antibiotic susceptibility pattern, multiple antibiotic resistance (MAR) index and prevalence of extended spectrum β-lactamases among clinical isolates of P. aerugino
Qin Liu1,2, Bin Wang1,3, Yu Xu1,4, Xiuyue Zhang1, Tao Zeng1* and Jianghong Ran1*

 

...17 tetra-nucleotide microsatellite loci to test parentage and kinship for a wild population with 29 individuals from 10 different families (A-J). The 17 loci with the mean polymorphic information content (PIC) of 0.566 and the combined probability of exclusion for the second parent (CPE2 = 99.98%) met the minimum standard of international human paternity test. Among all 18 identified birdlings and helpers, bi-parents of eight offspring were successfully inferr...
Muhammad Hamayoon Khan*, Niaz Hussain Khuhro, Muhammad Awais, Muhammad Usman Asif and Raza Muhammad
 
 
... (Saunders) and B. dorsalis (Hendel) cause severe qualitative and quantitative damages to various fruits. The present study was executed to record the population dynamics of these fruit fly species in guava and mango orchards with respect to meteorological factors using methyl eugenol-baited traps. The results revealed that population of both the species highly fluctuated round the year. B. zonata appeared to be the most abundant species both in ...
Mashal Malik and Mudassar Nawaz Khan*
 
...). Five SSR primers (Soy satt 001, Soy satt 005, Soy satt148, Soy satt160, Soy satt171) which were previously reported to be highly polymorphic and mapped on different linkage groups, were used for analyzing genetic variations in soybean. All the primers were amplified with two different-sized alleles against each prim...
Arsalan Khan1, Aminullah Khan2, Dil Fayaz Khan2, Sajid Khan1, Anjum1, Waqar Ali3, Habib Akbar1 and Ahmad Khan1*
 
 
...erimental area was Loamy sand with Haplic yermosols soil series based on FAO classification. The experiment comprised of three tillage practices i.e. Shallow tillage (ST), Conventional tillage (CT) and Deep tillage (DT) and eight nitrogen treatments (Control, 100% Urea, 100% FYM, 100% PM, 50% Urea/FYM, 50% Urea/PM, 50% FYM/PM and 33.33% each PM/FYM/Urea). These ratios provided a total of 120 kg N ha-1. The wheat cv. Pakhtunkhwa-2015 was sown with se...
Muhammad Zubair1, Habib Ahmad2*, Brian E. Hemphill3, Muhammad Tariq4 and Muzafar Shah5
...nsular India prehistoric samples of Indus valley Central Asia and South Asia. Inter sample affinities based upon pair wise MMD values were examined with neighbor-joining cluster analysis and multidimensional scaling. The results remained highly consistent throughout different data reduction techniques. The consistency in phenetic affinities was even evident when they were considered in the backdrop of living ethnic groups of...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...s change and insect bioassays using Plutella xylostella larvae as the host resulted in the isolation of a collection of mutants with increased virulence. One mutant, designated, MlUV-40b showed 3.3-fold increase in virulence as compared to the wild type parent, with an LC50 = 0.3 ×105 conidia/ml ve...
Wali Muhammad1*, Humayun Javed1, Munir Ahmad1 and Tariq Mukhtar2
...ts long-term control and safety to the environment. In the present study, effect of four sowing dates was evaluated on the infestation of L. orbonalis on brinjal at two locations in the Pothwar region of Pakistan. The study revealed that sowing dates influenced infestation by brinjal shoot and fruit borer. Shoot infestation significantly varied on brinjal at both locations sown at different dates. The maximum average shoot infestation of 9.7% was observ...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...g analysis results of 46 samples, it showed that new mutation at g.1626G>T was only detected in one individual sample. Polymorphism of bLHR/HhaI gene in the present study was not significantly associated with S/C of Pasundan cows, since the genotype TT of the studied animal was found in the lowest of S/C value than other genotype.
...
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
...cycle using radioimmunoassay. The average length of the estrous cycle in Lohi sheep was 17.0±0.1 days and follicular and luteal phases were 4.6±0.2 and 11.3±0.2 days long, respectively. Estrous cycles had either three or four follicular waves; 3-wave cycles were more frequent than 4-waves (87vs.13%; p≤0.05, respectively). In 3-wave cycles (n=14), follicles (≤ 3mm) emerged on Days 0.7, 5.2 and 10.5. In 4-wave cycles (n=2), foll...
Muneer Abbas1*, Dilbar Hussain2, Muhammad Saleem2, Abdul Ghaffar2Sohail Abbas3, Niaz Hussain1 and Abdul Ghaffar1
.../i> and Bactrocera dorsalis (Diptera: Tephritidae) has endangered the trade of Pakistani fruits in foreign markets. Use of IPM is best way to control fruit fly by avoiding harmful insecticidal spray application in order to save natural enemies and environment. Experiment was conducted in three guava, citrus and mango producing districts of Punjab i.e. Faisalabad, Sargodha, To...
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
...ating (NM). Blood samples were collected at 1, 7, 15, 23, 35, and 60 days post NM. Levels of ISG15 and ISG17 mRNAs were assayed using real-time PCR, and serum progesterone (P4) concentrations were assayed using an ELISA kit. Pregnancy detection was performed by US on 23, 35, and 60 days post NM....
Ijaz Haider1*, Muhammad Akhtar1, Ali Noman2 and Muhammad Qasim3
...and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) on light trap. The effect of some abiotic factors like temperature, relative humidity and rainfall on the adult population of these pests was also studied. The population of these pests was monitored on the light trap from March to November from 2000 to 2014 in rice wheat cropping system. Yellow stem borer (YB), white stem borer (WB) and pink stem borer (PB) first appeared on the lig...
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
...tic marker, such as microsatellites. Based on our preliminary sequencing data, the present study describes the use of time-of-flight mass spectrometry (TOF-MS) to identify the locations of four new microsatellites in Qinghai Bamei pig (n=256), an indigenous breed of Chinese pig. TOF-MS demonstrated that the L1-(GT)n locus had three alleles: 152 bp, 164 bp, and 166 bp. The three other loci, L2-(AC)n, L3-...
Muhammad Tahir Waseem1, Abdul Majid Khan1*, Abdul Ghaffar2, Ayesha Iqbal1 and Rana Manzoor Ahmad1,3
...ed to have inhabited a mosaic of woodland and grassland with predominant C3 vegetation which latter shifted to C3/C4 grasses. For this purpose, fifteen dental samples (Five for each Hipparion species) from well dated late Miocene localities between 8.8-7.7 Ma are analyzed. All the δ13C values (V-PDB) are in range of -11.27‰ to -3.36‰ which represent that ...
Ying Liu1, Shankun Liu1, Hui Wang2 and Weihua Su2,*
...nancy (19 day) the blood sample was collected via puncturing the retro arbital and collecting the fetus from all the groups. The rats, fetus weight and placental weight were calculated. The blood glucose level, serum, lipids, antioxidant, cytokines and C-peptide was recorded. GDM group rats showed the lower weight of pregnant rats, fetal and placental weight as compared to other group rats. Caffeic acid treated group rats exhibited the modulated level of blood...
Ahmad Kamran Khan1, Muhammad Hamid Bashir2*, Shanza Ahmed3, Muhammad Amjad Bashir1, Shahbaz Ali1, Syeda Amber Hameed4Munaza Batool5, Irfan Ahmed6 and Muhammad Naeem Khan7
...f Punjab, Pakistan. Soil sampling was done after two months interval for a year 2014. Family richness, abundance and Shannon diversity indexes were higher in undisturbed soil as compared to the disturbed one in all the localities, while low variability with respect to evenness had been found in two different types of soil. The highest diversity of soil inhabiting Prostigmata were recorded in undisturbed type of soil in the month of June at Fai

Saleem Maseeh Bhatti1*, Aslam Khan Gadehi1, Inayatullah Rajpar1, Muhammad Nawaz Kandhro2, Mukesh Kumar Soothar1 and Zohaib Ur Rehman Bughio1

 

 

... against a range of NaCl salt-waters (EC 2.0, 4.0, 6.0 and 8.0 dS m-1) and canal water (EC 0.3 dS m-1). Spinach plants were irrigated with defined EC levels at 80% field capacity of a clay loam, non-saline soil. Results indicated that the growth and yield parameters of spinach genotypes were constantly decreased with an increase in EC levels of irrigation water. The plants irrigated with EC 6.0 and 8.0 ...
Baochao Liao1,2, Xiujuan Shan2,3* and Yunlong Chen2,3
...ovy lifecycle (egg, yolk sac larva, exogenous feeding larva, juvenile, and adult) and predicted how individual-level processes affect energy dynamics at higher levels of biological organization. We estimated primary model parameters (e.g., energy conductance, ; allocation coefficient, κ; and volume-specific somatic maintenance, [M]), and for a 5-year simulation, calculated a mean population growth rate (r...
Muhammad Qayash Khan1,2, Muhammad Zubair Anjum2, Muhammad Adnan3, Abbas Khan1, Hafsa Zahid1, Javed Nawab4, Sher Zaman Safi5Muhammad Ishaq Ali Shah6, Atif Kamil7 and Abid Ali1*
...khtunkhwa, Pakistan. The samples were morphologically identified, its mitochondrial DNA was extracted and subjected to molecular characterization by amplifying and sequencing the mitochondrial cytochrome c oxidase 1 (CO1) gene. The sequencing data revealed rich genetic diversity among fish specimens belonging to the genus Schizothorax. For the genus Tor, as was commonly believed of having two species (Tor putitora and Tor macrole...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 
...medium. Quantification assays such as test tubes revealed significantly (p<0.001) strong biofilm after 5 days with significantly increased planktonic cells (after 3 days) and increased loosely bound cells (after 5 days). Similarly, air liquid interface coverslip indicated significant increase in biofilm after 1 day. Comparison of antibiofilm effect using proteolytic enzymes indicated that although all enzymes resulted in significant decrease (p<0.05) in ...
Muhammad Akram Muneer1, Khalid Munir1, Ghulam Abbas1’*, Isra Munir1Mumtaz Ahmad Khan1, Asif Iqbal1, Munsoor ud Din Ahmad1, Muhammad Arshad Javid2, Zareen Fatima1 and Maria Arshad1
...uesduringlastthree decadesas these infectionscaused quitelargenumberofhumandeathsworldwide.Coronavirusesaresingle-strandedpositive sense RNA viruses which mainly in past were considered responsible for high percentage of (around 30%) of common cold/flu cases. Viruses causing SARS, MERS and COVID-19 are members of family Coronavirdae. World Health ...

Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3

...osed. It is given a proposal of measures for further activities on the preservation and improvement of the state of animal genetic resources in Serbia.
...
Laura Alejandra Arriola-Mosqueda1, Yadira Jiménez-Lara2, Eugenia del Carmen Prieto-Avella1, Carlos Ricardo Cruz-Vazquez3, Roberto Montesinos-Matias4, Mauricio Valencia-Posadas2, Ana Martha Cruz-Avalos5, Jaime Molina-Ochoa6,7 and César Andrés Angel-Sahagún2,*
...rvae of Rhipicephalus sanguineus, as well as the LC50 on R. sanguineus larvae, were determined and the germination (at 18 and 24 h post-inoculation) and radial growth (after 14 days) of four strains of M. anisopliae. We evaluated the pathogenicity on the eggs and larvae of all tested strains of R. sanguineus at day 3 and 7. The LC50 of four...
Ammara Gull-e-Fareen1,2, Imran Bodlah1,*, Muhammad Adnan Bodlah3, Muhammad Tariq Rasheed1, Habib Ali4 and Muhammad Asif5
...ike scape, pedicel and basal part of antennal segment 3 slightly darker than remaining segments, scutellum and mesopleura mostly dark reddish with some darker areas, legs also reddish brown except tarsal segments lighter in colouration. Suspected trophic associations with Ischiodon scutellaris (F.) via Aphis nerii onCalotropis procera (Ait.) Ait and Tinocallis kahawaluokalani (Kirkaldy) ...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...l disease in 2017. Blood samples were collected from all age groups patients visiting hospital labs and District Head Quarter Laboratory who were recommended for diagnosis of malaria. About 1050 patients were examined and malaria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found infected with malaria parasite. Comparative Data was also collected from other labs of District Swat which accum...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
... Punjab, Pakistan. Blood samples (n=400) taken from local (n=200) and exotic (n=200) breeds of cattle were processed for complete blood count. Based on lymphocyte counts, samples were divided into two groups (A, lymphocytosis, B, normal lymphocytes) and tested through ELISA and nested PCR for serological investigations and molecular characterization resp...
Nosheen Basharat1, Usman Waheed2,3*, Muhammad Arshad1, Noor e Saba4, Iram Masood1, Akhlaaq Wazeer1, Ahmad Farooq1, Sadaf Moneeba1Abdul Rauf5 and Hasan Abbas Zaheer2,3

 

... to June 2017. Total 282 samples were selected after routine blood screening of Hepatitis B and C, of which 75 were HCV positive (asymptomatic), 75 were HBV positive (asymptomatic) and 132 were from healthy (without HCV and HBV) voluntary blood donors. Selected samples were tested for TTV presence by ELISA and PCR assa...
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
...d. A total number of 100 samples of mastitic milk from native lactating buffaloes’ breed were collected aseptically from 4 private farms in Cairo-Alexandria desert road in the north part of the Egypt and subjected to bacteriological and molecular examination for M. bovis followed by their antibiogram profile. Seven (7%) isolates were identified as M. bovis from totally examined 100 milk sample
Rahmat Ullah Khan, Asif Sadam and Sajid Mahmood*
...ation are recommended to save the population of chakor.
...
Sana Khan1*, Faiza Aman1, Muhammad Ismaeel2, Zafar Ali2, Mehboob Alam1, Sadeed Iqbal1 and Taimur Khan3
 
...ameters of pea (Pisum sativum L.) cultivars at Agricultural Research Station, Swabi-Pakistan, during the year 2016-17. The plots were prepared according to Randomized Complete Block Design (RCBD) with split plot arrangement that were replicated three times. Phosphorus levels (0, 30. 60, 90, and 120 kg ha-1) were subjected to main plots and cultivars (Meteor, Samrina Zard, Leena Pak and Pea-2009) to sub plots. Data was collected on growth and ...

Faiza Aman1,*, Neelam Ara1 and Syed Mehar Ali Shah2

 

 

...ifty-seven pea (Pisum sativum L.) genotypes including 50 accessions and seven varieties were evaluated for the nine quantitative traits to assess the variability and association patterns for the characters under study. The genotypes were grown in plots using Randomized Complete Block Design (RCBD) at Horticulture Research Farm, The University of Agriculture Peshawar during 2016-2017 pea growing season. All the studied parameters showed significant (p&le...
Saba Shabeer1, Riffat Tahira2 and Atif Jamal3*
 
...pathogenic fungi is Fusarium spp. Fusarium species are very well-known soil-inhabiting fungi that cause many economically important diseases of crops.Many species are included in the Fusarium genus, which are not only pathogenic to plants but also cause different diseases in humans and livestock.Apart from diseases, one of the most dangerous characteristics o...
Hafiz Hammad1, Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Abid Niaz4 and Muhammad Arif4
Sheza Shehzadi1, Mohammad Umar Farooq2*, Rukhsana Kausar1, Ijaz Ali1*, Muhammad Arshad Ullah3 and Maqbool Shahbaz4
...1 small ruminants on the same biomass and also maximum forage production recorded 3882 kg/ha and it sequester carbon to up to 3560 kg C/ha in the given interval. Increase in fiber and ash content also justify the increase in biomass yield of the plant after interval of 60 days. The study indicates that Mott grass or Napier grass based on its high biomass yield is an effective approach in maintaining the fodder quality for animals on the sustainable basis and b...
Kartika Noerwijati1*, Sholihin Sholihin1, Tinuk Sri Wahyuni1, Rohmad Budiono2, Nguyen Van Minh3, Roy Hendroko Setyobudi4, Zane Vincēviča-Gaile5 and Lulu Husna6
 
 
...nes from 42 families. Cassava selection in the early stages involved a large number of clones, therefore cluster analysis (the K-means cluster analysis method) was carried out to classify clones based on tuber yield and harvest index and to facilitate the selection process. The aim of this study wasto select high yielding cassava clones as selection material for the next stage of selection in the highlands. The F1...
Muhammad Tariq Chaudhary1, Saleem Ashraf 2, Muhammad Yaseen3 and Muhammad Luqman3
 
 
...re found complete, thus, sample size was reduced to 237. Effective Schools Model presented by Lezotte (2010)” was employed as theoretical framework of this study. Collected data were analyzed through Statistical Package for Social Sciences (SPSS). Results unveiled that average age of respondents was 25.03 years. Maximum respondents (31.96%) had received DAS from IATI, Sargodha while least contribution came from University of Agriculture Fai
Sana* and Abbas Ullah Jan
 
...   collected from a sample of 275 farm households, and the involvement level of their children in agriculture was quantified over three points Likertscale(Neverinvolved,Part-time involved and Full-time involved). Data analysis revealed that 34.7 percent of children of the sampled farm households were full-time involved and 41.9 percent were part-time involved in different agricultural activit...
Raheel Saqib1*, Muhammad Luqman2, Saleem Ashraf3, Tahir Munir Butt4, Abdur Rehman5, Imtiaz Hussain6 and Muhammad Umer Mehmood2
 
 
... drawn randomly. A total sample size of the study was 110 respondents (rural female engaged in different farming activities). Data were collected through pre-tested interview schedule and analyzed using SPSS. Results showed that an overwhelming majority of respondents (89.1%) were perceived that family farming played a significant role in improving their livelihoods on a sustainable basis. A large majority (84.6%) of respondents reported the positive role of f...

Dilara Devranoglu, Ilksen Tavaci, Gonul Filoglu and Ozlem Bulbul*

...en individual based on a sample of DNA alone. In this study, we tested degraded DNA samples to determine the eye color prediction accuracy of the IrisPlex system. We used five years old bloodstains and UVC exposed (30 and 60 min) DNA samples. PCR and electrophoresis were performed. The multinomial logistic regression model statistics is applied for eye color prediction. We observed allele ...

Muhammad Ihtisham1, Noor Amjad2, Muhammad Nauman3, Asghar Ali3, Khawar Riaz3, Muhammad Sajid3, Muhammad Owais Shahid*3 

 

...t and its seeds have the same issue. In this experiment, seeds of Sophora secondiflora were treated with H2SO4 and then sown at different dates. Both H2SO4 scarification and sowing time had a significant effect on germination and seedling growth. The seeds scarified with sulfuric acid for 135 min gave the maximum plant height (25.42 cm), leaves plant-1 (34.45), leaf area (8.00 cm2), stem thickness (0.47 cm), fresh leaves weight (3.31 g), fresh stem weight (1.9...

Muhammad Yaseen1, Xu Shiwei2, Yu Wen2, Muhammad Luqman1*, Raheel Saqib3, Muhammad Ameen4, Sadia Hassan5 and Tahir Munir Butt6

... latest information necessary for agricultural productivity. This study aimed to explore the patterns of farmers to access and receive information from different sources. A well-structured and expert reviewed interview schedule was used to collect data from farmers from Huailai county. A total of 122 interviews were conducted for the collection of data. Data were recorded using EpiData software program and a logistic regression model was applied using the comp...
Yuanyuan Zhang1*, Liping Song1, Hui Guo2, Jun Wu1, Xiaoli Wang1 and Fangbin Yao1
...ial was carried out. A basal feed was supplemented with 0 (control), 30, 60, 120 and 240 mg/kg curcumin to formulate five experimental feeds. At the end of the feeding trial, the growth performance was determined. Subsequently, CCl4 was used for the model experiment. The plasma and liver were collected for the test after 72 h. Results showed that there was a significant (P<0.05) increase in weight gain rate (WG) and a special growth rate (...
Jiawei Hong1,2, Mingqiang Chen 1,2, Zhenghua Deng1,2, Youning Li1,2 and Yu Wang1,2*
...reatments at different dosages (0, 5, and 10mg/L ENR) using 16S rDNA gene sequencing. A total of 11 phyla comprising 76 genera were detected. At the phylum level, the relative abundance of Proteobacteria increased (from 34.96% to 77.31%) with the increasing of enrofloxacin exposure dosage. The dominant position of Tenericutes was replaced by Proteobacteria, and in parallel the proportion of Tenericutes slumped to 3.85%. At t...
Sadaf Javaria1, Anum Marwat1, Muhammad Nadeem2, Mehwish Zerlasht1, Aiman Kareem3, Iqra Rubab1 and Masooma Munir4*
...tion showed that all the samples were in an acceptable range. However, Mix fruit leather with 50 % apple puree and 50% peach puree was liked the most by the panelists.
...
Asima Batool1, Ghulam Abbas2*, Zuhair Hasnain3, Nasira Perveen4 and Muhammad Naeem Akhtar5
...riculture University, Faisalabad. Germinated maize seedlings / plants were placed in the glass fitted canopies, where canopy temperature was found 7-10°C higher than ambient temperature along with light reflection was enough for optimum photosynthesis. The plants were raised under heat stress environment for 15 days and harvested. Data regarding seedling parameters
Tariq Ali Mastoi1, Zulfiqar Ali Mastoi2*, Zahoor Ahmed Khetran3, Ghulam Hussain Alizai2, Binish Baig4, Mitha Khan2 and Syed Jahangir Shah1
 
 
...posively by using random sampling techniques to investigate eligibility, education and obstacles of the respondents for obtaining micro finance loan from Zarai Taraqiati Bank Limited (ZTBL). Out of selected districts; ten respondents were chosen randomly who obtained micro finance loans. The information of the selected districts and respondents was obtained (ZTBL). Thus, the respondents were interviewed personally and necessa
Tasneem Shah*, Muhammad Tariq and Dost Muhammad
...rtile soil. Surface soil samples from a 4 years long field experiment were collected to assess the effect of added biochar (0, 50, 90 and 130 t ha-1) and chemical fertilizers (viz., urea for N, single superphosphate for P and potassium chloride for K) on soil respiration and N mineralization during lab incubation experiment under wheat-mungbean and wheat-maize cropping systems. The rate of CO2 evolution increased significantly (P<0.05)...
Muhammad Nawaz Kandhro1*, Nadia Mangrio1, Aijaz Ahmed Soomro1, Zia-ul-Hassan Shah2, Ghulam Sughra Mangrio3, Nihaluddin Mari4, Zulfiqar Ali Abbasi1 and Shazia Parveen Tunio5
 
 
Md. Sabbir Ahamed1, Md. Rayhan Chowdhury1, Md. Atik Mas-ud1, Sujat Ahmed2, Md. Shahadat Hossain1 and Mohammad Nurul Matin1
 
 
Yongyun Zhang1, 2, Xinyang Fan1, Fangting Zhou1, Weizhen Li3, Yina Ouyang1,4 and Yongwang Miao1*
...types of buffalo was the same in length, which contained 645 nucleotides and encoded a peptide composing of 214 residues. A total of 5 single nucleotide polymorphisms (SNPs) was identified in two types of buffalo. Among them, c.516T>C and c.578C>T were observed only in river buffalo, while c.175A>G, c.580T>C and c.609T>G were found only in swamp buffalo. The c.175A>G, c.578C>T and c.580T>C were non-synonymous, which led to substitutions...

 Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*

...reas of the body. Two consanguineous families were enrolled from Basti Mochi Wala, Mouza Gulab Shah in Muzaffargarh District of Punjab (Pakistan) having multiple siblings suffering from alopecia. The aim of this study was to find out the genetic mutation(s) in hairless (HR) gene, if any, in the enrolled subjects. A questionnaire was filled for each subject on the sampling site in order to collect epidemiological data ...
Kamran Baksh Soomro1*, Sina Alaghmand2, Sanyogita Andriyas3, Muhammad Rehmatullah Khan4, Naveedullah5 and Amin Talei6
 
 
... agriculture, the use of saline water often emerges as an alternate source of irrigation. However, one hardly knows the economic implications of such recommendations. In this regard, the effect of saline irrigation on the economics of crop yield deserves scientific inquiry. A field experiment was conducted during 2018-19 to study the impact of saline water on the economics of bitter gourd ...
Muhammad Ilyas Khan*, Ihsan Mabood Qazi and Mohammad Ayub
 
 
...hich may be useful in versatile food products, especially noodles. 

...
Haseeb Khattak1, Imran Ahmad1, Abdul Basit1*, Izhar Ullah1,2, Syed Tanveer Shah1, Humaira Wasila3, Inayat Ullah4, Intizar Ahmad1 and Noman Ahmad1
 
Roland Eric Yessinou1*, Alain Richi Kamga Waladjo2, Nestor Noudeke1, Ignace Dramou3, Justin Adinsi1, Victorien Tamègnon Dougnon4, Elsie Yélognisê Sangnidjo5, Razack Osse5, Alfred Dansou6 and Souaïbou Farougou1
...line-height: normal;">Lassa virus (LASV) is a highly prevalent arenavirus that affects two to three million people in West Africa. A retrospective was conducted review through literatures search using the AGORA, PubMed, Science Direct, Scopus, Researchgate and Google Scholar Databases on Lassa fever (LF) from West Africa. A total of 34 articles were studied from 11 countries. Studies were categorized by host and country, and...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...9.3% -100%). The main causative agent of recent ND outbreaks in vaccinated broiler flocks in Menofia governorate, Egypt was found to belong to very virulent genotype VII. This strain was genetically identical to Egyptian genotype VII isolates isolated in the last period.
...
Ryan Septa Kurnia1* and Radiana Dhewayani Antarianto2
 

...helium is one of the mucosal epithelial which functions as protective and adaptive barrier against continuously inhaled substances including pathogens and allergens. Current research about mechanism of disease and drug development is conducted in 2D cell culture or in animal models. The 2D cell culture models poorly imitate the condition in vivo and provide limited utility due to mimic tissue physiology in multicellular organisms. Although animal models...
Hafiz Tassawar Abbas1*, Tamoor Khan1, Ghulam Khaliq2, Muhammad Aqeel Sarwar3, Muhammad Rashid4, Intazar Ali5, Muhammad Abuzar Jaffar2, Ghulam Ali Bugti6 and Muhammad Waseem4
 

Hafiz Imran Iqbal1*, Zahir Ahmad Zahir2, Obaidur Rehman1, Rizwan Khalid1*, Abdul Waheed1, Raja Abad Raza1, Shahid Saleem1, Muhammad Rashid1, Sarosh Tariq Alvi1 and Asia Munir1
 

...of maize plant affect by saline stress conditions. Salt stress can be reduced through beneficial rhizobacteria that may increase the growth of plants. An experiment in pot was conducted to assess the effect of combined use of rhizobia and plant growth promoting rhizobacteria (PGPR) (containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase) on the growth and yield of maize under salt affected conditions. In pots three lev...

 Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2

...five equal aliquots. The samples were diluted with a solution having different additives at 37 °C. The first two groups contained L-Cystine as an additive (2 and 4 mM), the next two groups had methionine as an additive (2 and 4 mM), and the last group had no additives (control). After dilution, the semen samples were cooled at 5 °C for 2 h and then stored in liquid nitrogen. For further evaluation, the
Aylin Celile Oluk1*, Ugur Serbester2, Murat Reis Akkaya3 and Oya Berkay Karaca4
...rbons and terpenes. Milk samples were collected at the early and late lactation periods from Hatay and Kilis, Turkey. During both periods, the goats consumed a diversity of plant species. Milk collected from Kilis had 15.27-16.10% total solids,5.70-6.50% fat and 4.80-5.10% protein. The lactose content did not differ significantly between milk samples from the two locations (3.90-4.20%). Total unsa
A.E.S. Patrick1,*, S. Kuganathan2 and U. Edirisinghe3
...lettinus, Puntius sarana and Heteropneustes fossilis. Only Channa striata (b= 2.996, p=0.396) and females of Puntius dorsalis (b= 3.076, p=0.234) appeared in the catch throughout the study period and showed a strong correlation between length and weight. Combined sexes of, Glossogobius giuris (b= 3.178, p=0.216), Labeo dussumieri (b= 2.752, p=0.069) an...
BamideleAkinsanya1, Isaac O Ayanda2,*, Benson Onwusa1 and Joseph K Saliu1
...agoon. One hundred (100) samples of Heterotis niloticus were investigated for parasitic infection for a duration of six months, while the parasites encountered were used as sentinel organisms to check for the presence of PAH and BTEX. GC-MS was used to analyse for the presence of these chemicals, while parasite identification was done using conventional method. Only one parasite species, Teneuisentis niloticus, an acanthocephalan was recovered. T...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2,  Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*

Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6

... antibodies (IgM) were assayed using enzyme-linked immunosorbent assay HDV IgM ELISA Kit (MyBiosource, Inc, USA). The result was interpreted according to manufacturer’s instructions. Ninety HBsAg positive patient were included (45 HBV/HIV, and 45 HBV-mono infected patients), 36...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...age. A total of 20 fecal samples were collected in the winter season of 2018 and 2019, all samples were serologically screening by antigen-capture ELISA, then molecular confirmation by RT-PCR targeted to nucleocapsid protein gene (N-gene) was carried out followed by phylogenetic analysis. Positive samples were isolated...
Titin Herawati1,*, Indra Adiwiguna1, Ayi Yustiati1, Iis Rostini1, Roy Hendroko Setyobudi2, Hafizan Juahir3 and Asep Sahidin1
 

...vation method and census sampling technique were applied. Research commenced from November 2017 to January 2018. Parameters measured are diversity index, dominance index, equitability index, and water quality. Data analysis employed comparative-descriptive method by mapping the species and comparing the diversity of fish living in both ends of Jatigede Dam. The results indicate that six families of fish are identified, consisting of 14 species. The fish divers...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
 

...58]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE. Each of the treatments was repeated three times. The sperm mortality and motilit...

Uwem E. George1, Oluwadamilola A. Arowolo2, Oluseyi A. Olayinka3, Ijeoma M. Ifeorah4, Temitope O.C. Faleye3,5, Bolaji Oluremi6, Arthur O. Oragwa7, Ewean C. Omoruyi8, Emmanuel E. Udoh9, Oluwadamilola G. Osasona1,10, Emmanuel Donbraye11, Olufunke P. Adeniji12, Olubusuyi M. Adewumi3,13* and Johnson A. Adeniji3,13,14

...c administration and/or usage in both humans and farm animals have led to a consistent interference and contamination of biomes and ecobiomes. These resultantly give platforms for positive selection of resistant pathogens and high levels of Antibiotic Resistance Genes (ARGs). We examined Nigerian Chimpanzees in Boki Afi Mountain Wildlife Sanctuary, Cross-River State, Nigeria, to detect ARGs. Faecal samples from 15 Chimpanzee...

Masood Sadiq Butt1, Sadia Aslam1,2, Rizwan Shukat1,*, Syed Qamar Abbas1, Muhammad Issa Khan1, Shadab Shaukat3 and Muhammad Shahid4

...uction of protein hydrolysate with enhanced functionality. Minced rohu (Labeo rohita) waste mainly comprising of head, tail, fins and skin was considered as raw material to prepare protein hydrolysate using flavorzyme enzyme. The results showed that response surface methodology was employed to optimize the reaction conditions (temperature, time, pH and enzyme to substrate ratio) for enzymatic hydrolysis of fish protei...
Canan Vejselova Sezer*
...lucidated by using MTT assay for cytotoxicity, annexin-V analyses for cell death mechanism and confocal and transmission electron microscopies for morphological and ultrastructural changes. Based upon our findings, it can be concluded that silymarin-loaded solid lipid nanoparticles significantly reduced the growth of A549 and MCF-7 cells compared to silymarin. Also these nanoparticles induced apoptosis both in A549 and MCF-7 cells in higher percentages than th...
Rukhsar Ali* and Ali Muhammad
 

... during storage. Control sample without preservatives (MLBPr0) blemished within one month of storage period. Different treatments preserved with food chemical preservatives were statistically significant during storage period of six months at (p <0.05). From this research work, it has been accomplished that the effect of collective dosage of chemical food preservatives i.e. 0.033% PS, 0.033% SB, and 0.01% KMS o...

Oluseyi A. Olayinka1, Temitope O.C. Faleye2, Oladipo O. Omotosho3, Oladapo A. Odukaye3, Bolaji Oluremi4, Ibipeju H. Ibitoye1, Oludayo O. Ope-Ewe1, Uwem E. George5, Oluwadamilola A. Arowolo6, Ijeoma M. Ifeorah7, Ewean C. Omoruyi8, Emmanuel Donbraye9, Olufunke P. Adeniji10, Olubusuyi M. Adewumi1,11* and Johnson A. Adeniji1,11,12

...is study was designed to sample the fecal Resistome of Pigs from a small-scale Piggery in Ibadan, South-West Nigeria. Three fecal pellets were randomly picked from the floor of unit pens containing a minimum of three pigs per unit in selected piggery in Ibadan, Nigeria. The samples were pooled and resuspended in phosphate buffered saline. The suspension was then subjected to nucleic acid e...

Monoar Pallab*, Moktadir Reza and Debashish Sarker

...esia. A small portion of saline tube was inserted into the teat cannel to maintain the flow of milk and prevent adhesion during healing. Muscle and skin were closed by interrupted and continuous sutures respectively. The animal recovered without any complications three weeks post-operatively.

...

Mustasim Famous1*, Asim Chakrabartty Aditya2, Shahabuddin Ahmed3 and Sabuj Sutradhar2

Safdar Hussain1, Muhammad Naeem Mushtaq5, Ali Bakhsh3, Muhammad Mudassar Maqbool1, Muhammad Sarwar1, Muhammad Jan4*, Muhammad Abdul Qayyum2 and Arif Husain2

...le dwarf-1, Aas-2011; Faisalabad- 2008) were exposed to critical drought stage. The plants were exposed to normal irrigation, application of bio stimulants like 2µM ABA, 10 mM SA, 15% MLE and 10% MBLE were applied at grain filling stage but skipping irrigation. Maximum growth related parameters were observed by applying full irrigation. Comparing the bio stimulants application, the a...

Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3, Waqas Raza1* and Mahmood Ahmad Randhawa4

Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*

...mmunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein (OMP) on Nili Ravi buffaloes. Prepubertal Nili Ravi female buffaloes (N=18) of 10-12 months age in good health condition were divided into five treatment groups (n=3 each) and a control group (n=3). Treatment groups’ animals were exposed to P. multocida bacterial culture and its immunogens, i.e., LPS (oral and intravenous) and OMP (oral and subcutaneous). Animals were analyzed for the d...

Salma Javed and Samreen Khan*

...A). Two fungal species Fusarium oxysporum and Aspergillus niger were examined for their aptitude to assist the population rate of the said species. Nematodes were collected from soil around the rhizospheres of sponge gourd (Luffa cylindrical L.) field in the vicinity of National Nematological Research Centre, University of Karachi and further processed by Cobb’s sieving and Baermann’s funnel techniques. Specimens...

Aurang Zeb1, Abdur Rab1, Shujaat Ali1,2*, Ijaz Hussain2, Shah Masaud Khan2, Israr Ahmad1, Bilal Pervaiz Khan1, Shakila Umer3 and Muhammad Abbas1

...s i.e. location-1 (Torwarsak, Buner), location-2 (Rabat, Lower Dir), location-3 (Wartair, Malakand) and location-4 (Palai, Malakand), which were stored in three replications at room condition for 48 days and Complete Randomized Design was used during the post-harvest experiment. Data were recorded on fruit juice pH %, titratable acidity, percent juice content, Rind thickness, Rind weight loss, at 12 days intervals. The fruit juice pH showed an increasing trend...

Aamer Sattar1, Sadia Sultana1*, Abid Niaz1, Muhammad Aftab1, Ghulam Sarwar2, Irfan Rasheed3, Muhammad Shoaib4, Raheela Naz1, Amina Kalsom1, Nisa Mukhtar1, Farah Rasheed1, Arfan ul Haq1, Munazza Rafique5, Muhammad Arif1, Sarfraz Hussain1 and Jafar Salim6

...l Research Institute, Faisalabad. Four treatments (sub plots) viz; 0 fertilizer, 50% of recommended fertilizer, 75% of recommended fertilizer and 100% of recommended fertilizer were applied under split plot design repeated thrice with 3 major plots (methods of residues management) i. traditional practice by complete removing (C) whole crop biomass from field, ii. burnt (B) practice by burning the crop biomass in field after threshing and iii. Residue’s (...

Arshad Mehmood Khattak1, Saleem Ullah1*, Farida Anjum2, Hamid U. Shah1 and Sahib Alam1

...d can be eaten either as salad or cooked as leafy green and their grain can be consumed as curries. Samples of tender leaves, green pods, green grains, Mature pods and Mature grains of seven different Chickpea cultivars KK-1, KK-2, KK-3, Chattan, Lawaghir, Sheenghar, Fakhar-e-Thal, were obtained from Karak Research Station Ahmad Wala and evaluated for proximate composition and mineral contents. The data related to proximate composition and mineral profile show...

Muhammad Musa1*, Muhammad Riaz Gondal2, Aaqib Riaz3, Sikander Hayat2 and Iftikhar Haider1

Syed Mazuan, Syed Mohamed, Insyirah Ishak, Dzolkhifli Omar and Norhayu Asib*

...ysia is the bagworm, Metisa plana. Various spraying methods had been developed for controlling the bagworms. A ground study was conducted in FELDA Gunung Besout 04, Perak, with its main focus on mistblower application towards bagworms. Stihl SR420 mistblower was characterised by having 1.2L / min flow rate, produced volume median diameter of 80μm, and achieved spraying productivity of 2.58 hectares’ land size (approximately 350 oil palms) per man day....

Saleem Maseeh Bhatti1*, Muhammad Aslam Panhwar1, Zohaib ur Rehman Bughio1, Muhammad Saleem Sarki1, Allah Wadhayo Gandahi1, and Niaz Ahmed Wahocho2

...wberry (Fragaria x ananassa cv. Silder) at Nasarpur, Sindh, Pakistan during 2015-16. The foliar rates comprised of 33, 66, and 99 mg Zn L-1 (equivalent to 100, 200 and 300 mg ZnSO4 L-1) and distilled water (control treatment) and sprayed to strawberry plants before flower initiation. A continual increase in number of leaves, number and weight of berries, fresh weight of strawberry plants, and Zn concentration in strawberry f...

Mehwish Saleem1*, Maria Tahir1, Maryam Ilyas2 and Aqsa Malik2

...ent’s blood. Blood samples were collected from cancer patients from MAYO hospital LHR during the time period April 2018 to May 2018. Some cancer patients have increased calcium level in blood. Cancer is more prevalent in males (n=17) 57% than females (n=13)43%. The most frequent cancer type among males is blood 41cancer (n=5) 36%, lung cancer (n=3)   22% and oral cancer (n=3)22%. In females breast cancer (n=5) 32% is most prevalent, followed by...

Manzoor Hussain Memon1, Khalid Khan2, Anjum Parvez3, Sarfaraz Ahmed Shaikh4, Khan Mir Khan2, Guo Xiangyu5*, Mohammad Nasrullah6 and Saima Liaqat7

...urveyed using multistage sampling technique. Building on quantitative industry figures the study also highlighted various qualitative problems and factors which are responsible for the production, and profitability of the poultry industry in the district. The results of the study revealed that the unfair and unwarranted distribution of profits among producers, commission agent and retailers are the major impediment to the extension of the poultry industry in t...

Ch. Muhammad Rafiq1, Qasim Raza1*, Awais Riaz1, Misbah Hanif2, Wajiha Saeed2, Shawaiz Iqbal1, Tahir Hussain Awan1, Syed Sultan Ali1 and Muhammad Sabar1

...ogy has water and labour saving advantages over conventional transplanting culture, however, poor crop establishment due to reduced germination in variable field conditions greatly hampers its large-scale adaption. To address subordinate germination issues, we investigated the effects of five salicylic acid (SA) concentrations (0, 75, 150, 225 and 300 pp...

Fazal Wahid1, Arif Ullah Shah1, Muhammad Rahim2*, Fazal Dad1, Nadir Khan1, Saqib Ullah1, Yasir Ali1 and Sayed Ashfaq Ali Shah3

...as evaluated in the soil samples of agricultural area of the Urmar Bala, district Peshawar, Khyber Pakhtunkhwa, Pakistan. To solubilize the targeted metals from selected soil samples, a wet digestion procedure was adopted. The targeted metals in selected soil samples were in the ranges of: 1.37-2.00, 28.91-29.50, 1.62-1.82, 1.22-1.179 and 0.07-0.112 mg/kg for Cr, Fe, Ni, Pb and Co, respect...
Asim Faraz1*, Abdul Waheed1, Hafiz Muhammad Ishaq1 and  Muhammad Shahid Nabeel2 
...onomical and resulted in savings of PKR 16,137 and 15,213 in calve groups fed with 18% and 22% levels of protein, respectively.
...
Li Shengqing1,2,3, Zhang Xiyun2,3, Liu Shengcai2,3, Hu Guoyuan2, Fan Yuxia2,Liu Huaixin2, Wang Tingting2 and Zhang Yanming1*
...gh plot experiments. The safety of type D botulinum toxin toward nontarget animals, such as yaks, Tibetan sheep, dogs, vultures, and birds, is also tested. Results show that the toxin is encoded by a type D botulinum neurotoxin gene. The DNA and amino acid sequences of the full neurotoxin gene comprise 3831 base pairs and 1276 amino acid residues, respectively. The nucleic acid and protein sequences of the toxin exhibits 99% homology with those of a known type...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2
Mark Wen Han Hiew1 and Lawan Adamu1
... cryopreserved using the same procedure. Different concentrations of either cysteine (0, 0.25, 0.5, 1, 2, and 4mg/ml) or ascorbic acid (0, 0.25, 0.5, 1, 2, and 4mg/ml) were added to the semen of the first and second experiments. HF-20 containing no ascorbic acid and cysteine was used as a control. The post-thawed semen from all the samples were assessed for motility, oxidative stress, sperm membrane integrity, morphology, an...
Xinjian Wang1,2, Eryan Yang1,2 and Lizhi Zhou1,2*
...us scan and focal animal sampling was conducted to collect the behavior data. Foraging behavior is the major behavior in the wintering period, followed by rest behavior and vigilance behavior. In daily activity rhythms, the trough and peaks of foraging behavior appeared in the morning (09:00-10:00 h) and evening(16:00-17:00 h), which is just contrary to the rest behavior. Foraging and rest behaviors showed highly significant differences among all the daily tim...
Ahmed Saud Alsaqufi1, Sheikh Mustafizur Rahman2,3*, Roshmon Thomas Mathew2, Yousef Ahmed Alkhamis1,2, Md. Moshiur Rahman3,4 and Muhammed Aslam Pathiri2
...total of 108 larvae were sampled for phenotypic traits analysis. The results revealed that complete darkness (24D) significantly improved the overall growth parameters (total length, standard length, caudal peduncle length, anal fin length, caudal fin length, total body depth, dorsal fin length, pre dorsal distance, pre anal distance, pre ventral distance, head width, head length, eye diam...
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
...a pacos, and Homo sapiens. Expression of C. dromedarius IFNα cDNA in Escherichia coli revealed a fusion protein with a weight of 22.5 kDa after induction of expression with IPTG for 5 h. The recombinant IFNα was expressed in the form of inclusion bodies that were separated and solubilized in vitro and the protein was refolded using SDS and KCl. The folded protein is then purified using on Ni-NTA Agarose affinity chro...
Arsalan Rasheed1, Tahir Usman2,*, Sadaf Niaz1, Irfan Khattak2, Saira Gul2, Nawab Ali1,Naimat Ullah Khan2, Hazrat Ali2, and Mian Saeed Sarwar2
Xiujun Yao1, Haofeng Wang 2, Ligong Zhang3, Jingzhang Wu4 and Lijun Wang1*
...establish rat model of mesangial proliferative glomerulonephritis (MsPGN), and were equally divided into model group and total glucoside of paeony (TGP) group. TGP group was given 150 mg/kg TGP by gavage, normal group and model group were given the same amount of saline by gavage. After 12 weeks of intervention, 24-h urine protein quantity, serum levels of IL-6, IFN-γ, and TNF-&alpha...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
...ded in total alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) in liver and urea, and creatinine levels in kidneys, in Al exposed groups against control. In Al + antidote groups these deviations were less significant (P≤0.05). However, these morphometric and biochemical alterations improved to a great extent when onion extract was co-administered with Al. So it is concluded that Al cause...

Frederic Ndihokubwayo and Atakan Koç

...ield (MMY, kg), the milk samples were taken from these cows during the morning milking for the determination of fat content (FC, %), non-fat dry matter content (NFDMC, %) and somatic cell count (SCC, cell/ml). The means for BDS, TEHS, MMY, FC, NFDMC and Log10SCC were 3.30±0.048, 2.17 ± 0.038, 13.92 ± 0.315 kg, 3.58 ± 0.039%, 10.14 ± 0.032% and 5.22 ± 0.024 (165 958 cells/ml), respectively. The effects of farm and parit...
Abdul Muhaimin Wahab1, Basit Zeshan2,*, Naveed Ahmed2, Muhammad Afzal2 and Muhammad Naveed2
...sia. Eighty cloacal swab samples were collected from 4 different broiler chicken farms in district Tumpat, Machang and Bachok. The samples were processed for identification of C. jejuni followed by PCR to detect the presence of C. jejuni. Overall, 65% in total (80 samples) of cloacal swab samples showed positive reactions, where prevalence ...
J.Y. Bai1,*, R.T.D. Wu2,*, Q. Zhang2, D. Bao2, L.M. Dao2 and X.H. Tian3
...">In this study, 17 microsatellite markers were used to analyze the genetic polymorphism, genetic differentiation, gene flow and genetic distance of Alashan desert Bactrian camel, Alashan Gobi Bactrian camel, Sunite Bactrian camel and Qinghai Bactrian camel.The results indicated that the number of effective alleles of the four Bactrian camel varieties ranged from 2.7302 to 3.0524, and average theoretical heterozygosity and average polymorphic information conte...
Fuhua Zhang, Yishuang Yu and Shibao Wu*
...ot only easy to operate, safe, and noninvasive, but also very intuitive and efficient compared with hormone detection methods. Efficient detection methods such as B-ultrasonography are critical to avoid housing already-pregnant pangolins together with males, which may mate with the females again or stress them, resulting in abortion. This approach also helps to conserve male reproductive resources, thereby improving the reproductive efficiency of captive Sunda...
Naimat Ullah Khan1*, Muhammad Hassan Saleem2, Aneela Zameer Durrani2, Nisar Ahmad2, Muhammad Shafee3, Ayesha Hassan2, Mumtaz Ali Khan2 and Nadeem Rashid3
...n. A total of 1440 fecal samples were collected from goats, 120 samples per month from each of 3 districts for twelve months. Identification of oocysts was done through conventional acid fast ZN staining. Prevalence in District Bannu was 48/480 (10%) followed by District Lakki Marwat12.08 % and in Kohat 19.16 %, respectively. Overall prevalence in three Districts was 13.75%. The highest month wise percent prevalence was reco...
Saba Afzal1, Sohail Raza1,*, Masood Rabbani1, Sehrish Firyal2, Imran Altaf1 and Zahra Naeem1
...s determined using MTT assay. The antiviral activity of ivermectin was determined using viral inhibition assays and median tissue culture infectious dose (TCID50). The results showed that 2.5 µM concentration of the ivermectin is non-toxic to the Vero cells. At this concentration, PPRV titre was significantly reduced (p < 0.001) by two log to 103.0 TCID50/0.1 mL as compared to...
Rida Haroon Durrani1*, Fareeha Akhtar1, Ali Ahmad Sheikh1, Munazzah Maqbool1, Aleena Kokab1, Kiran Imtiaz2 and Muhammad Ilyas Riaz3
...of fish meat. A total 24 samples were procured during three different seasons from local retail shops (n=12) and supermarket (n=12) in order to observe the bacteria which are associated with meat spoilage. Total viable count of aerobic psychrophilic, mesophilic and thermophilic bacteria was performed through selective enrichment and culturing of samples to determine the diversity of bacteria. Staphylococcus aureus (42...
Khurram Goraya1,*, Qais ALRawahi1, Sultan ALBalushi1, Hani ALSaadi1, Sami ALRahbi1, Zahir ALAlawi1, Muhammad Hammad Hussain2 and Madad Hussain1
...le (Gazella subgutturosa marica). These antelopes are only kept in a few countries worldwide. Therefore, only limited information is available on their husbandry requirements and diseases occurring in these species. This study aimed to evaluate the causes of mortalities at WWR. During July to October 2019, fifty mortalities were observed at WWR. These mortalities consisted of 10 Arabian oryx and 40 Sand gazelles. All carcasses were subjected to the deta...
Aiguo Zhou1,2, Di Sun1,2,Shulin Liu 1,2, Yongyong Feng1,2, Yue Zhang3
Yanfeng Chen4, Shaolin Xie1,2* and Jixing Zou1,2*

 

... snakehead belong to the same species at the molecular level. Moreover, all the haplotypes were gathered together by the Neighbour-Joining (NJ) tree, further confirming that the two kinds of snakehead morphs belong to one species.
...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
...se, a total of 272 fecal samples were collected from sheep and lambs from four different zones of district Peshawar. Isolates were morphologically, biochemically and molecularly confirmed through Gram staining, gelatin liquefaction test (GLT) and polymerase chain reaction (PCR), respectively. Out of total, 85 (31.3%) were positive for C. perfringens type D on PCR. The results revealed 6-34% prevalence of C. perfringens type D in different regions...

Muhammad Yousif Jakhrai1, Ahmed Nawaz Tunio2, Ali Mujtaba Shah1*, Tarique Ahmed Khokhar1, Muhammad Mohsen Rahimoon1

...study. The scope of lumbosacral epidural analgesia and also its clinical application was studied. The most commonly anesthetic agents injected to produce high epidural analgesia in ovine and caprine species were 2% lidocaine and xylazine were included in this study. This study showed that administration of xylazine in high epidural anesthesia produce a significant analgesic and sedative effect for laparotomy (peritoneal) surgical interventions in ovine and cap...

Muhammad Afzal1, Muhammad Irfan Ullah1*, Muhammad Hamid Bashir2, Shah Najaf Mukhtar1, Muhammad Arshad1, Nimra Altaf1

...present study, extensive sampling was done to assess mites’ biodiversity in different citrus orchards of Kinnow, Musambi, and Feutrell’s early at tehsil Sahiwal Sargodha, Sillanwali, and Bhalwal of district Sargodha. Sampling was done at fifteen days interval to record the population dynamics and identification of citrus mites during 2018. A total 37,963 mites specimens were collected in which nine species were i...

Imrana Khushk1, Abdul Nabi Jatt2, Abdul Sattar Qureshi1*, Choudhary Haider Ali3, Muhammad Aqeel Bhutto1, Imran Ibrahim Khaskheli1, Sartar Iqbal Panhwer1

...ed on wheat straw hydrolysate and were monitored with regular interval until stable cell growth, glucose consumption, and, ethanol and lactic acid production. Fermentation performance of the adapted strain improved over parental strains. Ethanol and lactic acid yield reached to 90% and 75%, respectively, when using freshly pretreated wheat straw or wheat straw hydrolysate or rice straw as carbon source. On-site produced cell...

Aioub Sofizadeh1, Ehsan Allah Kalteh2, Shahin Saeedi3, Mulood Mohammadi Bavani4*

... A total of 111 scorpion samples were captured. All the samples belonged to the Buthidae family, including Mesobuthus eupeus (97.3%), Orthochirus farzanpayi (0.9%), and Mesobuthus caucasicus (1.8%). M. eupeus had the highest distribution and abundance in the region and was recognized as the leading cause of scorpion sting in the region. O. farzanpayi and M. caucasicus are new to the scorpion’s species of this province....

Attaullah Jan1*, Saleem Khan2, Iftikhar Alam1, Farzeen Khan3 and Muhammad Farooq4*

... Council of District Charsadda KPK. The study included 235 school-going children of both sex aged 5-10 years. A Pre-tested questionnaire was used for collection of data for the parameters: socio-demographics, anthropometrics, and dietary intake. Data on anthropometrics included measurement of weight, height and BMI. Age and anthropometrics (weight, height and BMI) were used for z-score calculation following WHO standards and using WHO AnthroPlus software. Diet...

Muhammad Adnan Islam1*, Zia-Ul-Haq2, Rana Shahzad Noor2, Matiullah Khan4, Muhammad Mohsin Ali1, Zulfiqar Ali3, Asif Ali Mirani3, Hafiz Sultan Mahmood1, Muzammil Husain1 and Badar Munir Khan Niazi1

...t drill even 50% DAP was saved with 10 % excess wheat yield. In previous study s results of the fertilizer band placement drill were very encouraging as compare to farmer practice (Rabii Drill). Farmers were reluctant to adopt this fertilizer saving drill due to its heavy weight, which make it difficult to operate by commonly available 50-40 hp tractors. Keeping in view all these issues a low-cost version of fertilizer band ...
Mohammed  Akinlabi Rufai1*, Abideen Akinkunmi Wahab2, Kamil Ayo Fasasi1, Quadri Olusegun Adeshina1 and Mary Tolulope Awotidebe1
... water (µg/l); one sample not treated with nematode was served as control. Biocidal efficacy was achieved by collecting soil samples, sterilized and kept into experimental plastic plates labelled as A, B, C, D, and E. This was further divided by weight into five equal portions labelled as A1-A5, B1-B5, C1-C5, D1-D5, and E1-E5, respectively. The observation was done after every twelve hours for five iterations, and the ...
Yawang Sun, Yongjiang Wu, Jingbo Chen, Zili Wang, Juncai Chen, You Yang and Guozhong Dong*
...amage induced by lipopolysaccharide (LPS) in bovine mammary epithelial cell line MAC-T cells. Cells were cultured for 48 h with different LPS levels (0, 0.1, 0.5, 2.5, 12.5, and 100 ng/mL). The results showed that cell viability was negatively correlated with LPS concentrations. The production of reactive oxygen species increased in a dose-depend manner. Cellular concentrations of oxidative damage markers were positively correlated with applied LPS concentrati...
Jen-Hong Chu1, Ya-Wen Lan2, Shyn-Shin Sheen3 and Angela Chien3*
...mum dietary n-3 highly unsaturated fatty acid (HUFA) (C20:5n-3 + C22:6n-3) requirement for L. canarium was 19.59% of total lipid. Lipase activity in soft body increased with an increasing dietary lipid level, but amylase activity was not significantly affected by the dietary lipid level. Based on a broken-line of the final weight of L. canarium larvae, the optimum dietary lipid levels were 5.6%.
...

Talat Farid Ahmed1*, Shamim-ul-Sibtain Shah1, Ashfaq Ahmed Sheikh2, Hashim Nisar Hashmi3, Muhammad Atiqullah Khan1 and Muhammad Azeem Afzal1

...s annually, resulted thousands of families would be beneficial, getting equitable irrigation water availability and maximum water would be utilized.

...

Syed Ismat Hussain1, Muhammad Ramzan Asi1, Habbib Anwar1, Faqeer Ahmad1, Ateeq ur Rehman2*, Muhammad Shahid3, Syed Atif Hasan Naqvi2*, Ummad ud Din Umar2, Muhammad Asif Zulfiqar4, Memuna Ijaz1 and Hafiz Usman Shakir1

Qaiser Shakeel1*, Rabia Tahir Bajwa1­, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4

...ere used to conduct bioassay through poisoned food technique with triplicates. The crude extract of all the plants showed a better inhibitory effects on the pathogen’s (P. digitatum) mycelial growth rather than fungicides. Among all the plant extracts, the best result was showed by garlic extract with 87% of mycelial growth inhibition followed by moringa with 82%, the best concentration is 100mg/mL. Besides, Ridomil gold showed the most effective control...

Arjun Bastola1*, Sachet Subedi2 and Malatee Bastola3

...of experimental site was sandy loam with a moderately acidic pH. Results revealed that genotype Rampur composite was early yielder but JKMH 502 was late in flowering. Rampur composite had higher ear height hence susceptible to stalk lodging than rest of the genotypes. Stalk lodging seen less in CP-808. Rajkumar had longer cob length and larger cob diameter while maximum number of rows per cob were noted in CP-858 genotype. Higher number of grains per rows was ...

Muhammad Adnan Fahad1, Muhammad Jamal Khan1, Sheraz Ahmed2* and Imtiaz Ali2

...e measured to find water saving from improved watercourses. Conveyance losses were measured in improved and unimproved watercourses by current meter and cutthroat flume, respectively. Farmers were interviewed to find out the effect of watercourse improvement on crop productivity and water management practices using questionnaire proforma. The losses in five improved watercourses (lined sections) were 9, 28, 5, 11 and 20% per 1000 meter, respectively while that...

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sher Muhammad3, Muhammad Latif4, Ayesha Zafar1, Imran Shehzad1, Sarfraz Hussain5, Aneela Riaz6 and Abid Niaz2

...ents. At maturity, plant samples of maize were collected from all pots. Laboratory analysis for collected plant samples was carried out. Data were statistically analyzed. Results indicated that treatment (T8) produce maximum nitrogen (3.27%), phosphorus (1.0%) and potassium (2.85%) concentration in shoots of maize plants. The same trend of improvement was noted for maximum nitrogen (3.16%)...

Taqi Raza1*, Kashif Nazir Qureshi2, Shakeel Imran2, Neal Samuel Eash3 and Imma Bortone4

..., TSS and TDS). Effluent samples were collected from the discharge of industries entering into main wastewater collecting channel located in Faisalabad city. Heavy metals contents were determined via atomic absorption spectrophotometry. Results showed that the highest pH (11.06) was recorded in textile effluent while EC (7.89mS/cm), TSS (1185.55mg/L) and TDS (6317.33mg/L) were found highest in chemical industrial effluents. ...

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sarfraz Hussain3, Ayesha Zafar1, Imran Shehzad1 and Aneela Riaz4

...eness of soil. Combined usage of poultry waste material and chemical fertilizer improved maize growth and health of soil. This experiment comprised of 11 treatments applied under completely randomized design like, T1 = chemical fertilizers at recommended rate, T2 = chemical fertilizers at half recommended rate, T3 = waste of poultry at the rate of 5 ton/ha, T4 = waste of poultry at the rate of 7.5 ton/ha, T5 = waste of poultry at the rate of 10 ton/ha, T6 = T1...
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...tic goats. The purposive sampling technique was applied to collect n=200 milk samples from different regions of goat populated areas of district Faisalabad-Pakistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of pathogenic strains of S. aureus through micr...
Yahong Guo1,3, Zeqin Fu1,3, Jiantong Feng1,3, Chengrui Yan1,3, Yingying Ye1,3*, Kaida Xu2 and Baoying Guo1,3
...gested that only the FZW sample showed significant divergence from the others. The wild and cultured ZS and WZ specimens groups were similar, and the wild and cultured ND specimens groups were crossed. Thus, we need to establish genetic protection units for M. unguiculatus, limit the selection of breeding parents, and maintain a high-quality germplasm bank for M. unguiculatus.
...

Muhammad Khalid Mukhtar*, Sania Waliat, Shafaat Yar Khan, Naila Amjad

...on were used in the bioassays. The results showed that LC50 for the lab strain against bifenthrin and dimethoate was 11.068 µg/ml and 8.879 µg/ml, respectively. LC50 for field strains, Bhalwal L1 and L2, against bifenthrin was 96.184 µg/ml and 130.903 µg/ml, respectively whereas against dimethoate it was 74.340 µg/ml and 84.531 µg/ml, respectively. The resistance ratios for field strains of locations 1 and 2 against bifenthr...
Suman Bhattarai1*, Subodh Raj Pandey2, Jaya Prakash Dutta3, Meghnath Timalsena4 and Rajendra Bam5
...terview by simple random sampling technique. Interview using semi-structured questionnaire schedule was carried out for primary data. Cobb Douglas production function, multiple regression, and paired t-test were used for the analysis of collected data. Multiple regression model revealed that family labor (p=0.68), age(p=0.007), family size (p=0.001), experience (p=0.007), harvesting (p=0.000) and maintenance of flowering plants (p=0.057) have significant effec...

Muhammad Suhail Ibrahim1*, Asif Ahmad1, Anwaar Ahmed1, Amer Mumtaz2, Muhammad Javaid Asad3, Saqib Jabbar2, Ahmad Mujtaba1 and Muhammad Nadeem4

...xt-align: justify;">Food safety refers to the potentially hazardous agents or contaminants present in food that can cause food-borne illness. These extraneous agents are causing morbidity and mortality. Contamination of food by various agents includes physical, chemical, and microbiological. Food safety ensures that what we eat, chew, or drink is safe for human health. The whole food chain...

Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Muhammad Zeeshan Manzoor1 and Aneela Riaz3

...cularly upon exposure to salt stress. Present research was performed using wheat variety Punjab-2011 with Randomized Complete Block Design (RCBD) under field conditions using three treatments and each replicated three times. Treatments were wheat florae were developed in plots having normal soil (EC= 2.32 dS/m) and salty soil (4.93 dS/m). Si as phosphate industry waste was applied @ 0, 50 and 100 µg Si/g soil. Results ...

Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Humaira Ramzan1, Mudassar Hafeez1, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Sher Muhammad4 and Muhammad Latif4

...is seriously affected by salinity worldwide. This research was performed in the research zone of Department of Soil and Environmental Sciences during the summer, 2018 in College of Agriculture, University of Sargodha, Sargodha, Pakistan. In the present study growth response of rice under normal and saline soil conditions was checked using Randomized Complete Block Design (RCBD) under divided plot arrangements with nine treat...

Qudsia Nazir1, Muhammad Aftab1*, Ghulam Sarwar2, Aneela Riaz3, Sarfraz Hussain1, Ifra Saleem1, Amina Kalsom1, Noor-Us-Sabah2, Mukkram Ali Tahir2, Ateeq-ur-Rehman4 and Muhammad Arif1

Hajira Nur-ul-Islam1, Ahmed Zia2*, Khurshaid Khan1, Hirad Ali3 and Abdul Aziz3

...khwa province. Out of 33 sampling sites, specimens of S. hypomelas were found from a single locality of the district. Identification of the specimens was done at National Insect Museum, Islamabad. Detailed description including synonyms, differential characters for the species, previous global records, habitat description, measurements of body parts and ecological data for the positive localities are provided. With the addition of this taxon, Anisoptera fauna ...

Muhammad Ahsan1*, Aneela Ramzan1, Muhammad Nafees1, Adnan Younis2, Muhammad Amin1, Gulzar Akhtar3, Khansa Saleem1 and Azka Sabeeh1

...nd extend vase life of Rosa hybrida cv. Freedom. The research was performed at the research area of Department of Horticultural Sciences, The Islamia University of Bahawalpur. There were 11 treatments comprising of honey (T1), sugar (T2), salicylic acid (T3), acetic acid (T4), indole acetic acid (IAA) (T5) and combination of all solutions with sugar. These treatments were compared with tap water (T0). There were four plants ...

Alim-un-Nisa1*, Sajila Hina1, Imran Kalim1, Mohammad Khalid Saeed1, Ijaz Ahmad1, Naseem Zahra1, Sania Mazhar1, Shahid Masood1, Muhammad Ashraf1, Qurat-ul-Ain Syed1 and Rabia Shad2

...g;C ensure that they are safe for consumption at both storage temperatures. 

...

Muhammad Shakil Ahmad1*, Muhammad Afzal1, Sylvain Nafiba Ouedraogo2 and Muhammad Zeeshan Majeed1

...ato crop in Sargodha, Faisalabad and Okara districts. This in-vitro study assessed the comparative feeding preference, food consumption and utilization rates and larval development of S. litura on different white and red skinned potato cultivars. Feeding bioassays revealed that all parameters including leaf weight loss, larval weight gain, relative food consumption and growth rates and conversion efficiency of the ingested f...

Mohammad Tariq Aziz* and Ayesha Khan

...earch area. District Charsadda was the universe of the study and Multi Stage Sampling technique was used to select respondents from three selected villages of Tehsil Shabqadar of District Charsadda, namely Hajizai, Katozai and Battagram. Through Proportional Allocation Technique a sample of 112 respondents on basis of 10% of total 1120 registered farmers was drawn to collect primary data t...

Chala Hailu Hussen and Fikiru Temesgen Geleta

.... By employing two-stage sampling techniques, data was collected from 160 farm households of selected Western Shewa zone districts, namely Ejersa-Lafo, and Bako-Tibe. Data was emanated from primary sources, which administrated by semi-structured questionnaire using personnel interview. On top of this, the focus group discussion was carried out on particular topics to supplement primary data source discussions. Both descripti...

Amjed Ali1, Kamran Arooj1, Bilal Ahmad Khan1*, Muhammad Ather Nadeem1­, Muhammad Imran1,2, Muhammad Ehsan Safdar1, Muhammad Mohsin Amin1, Amir Aziz3 and Muhammad Fraz Ali4

Maqsood Ahmad1, Muhammad Umer Chattha1, Imran Khan1, Muhammad Bilal Chattha2*, Faqir Hussain Anjum3, Sadia Afzal4, Muhammad Faran1, Fiaz Hussain3, Muhammad Talha Aslam1, Abdul Jabbar5, Muhammad Sultan Ali Bazmi5, Mahnoor Mehmood6 and Muhammad Umair Hassan1

...ersity of Agriculture Faisalabad. The experiment consisted of different sowing dates (SD); 25th April, 5th May and 15th May and variable mungbean cultivars Azri Mung-2006, NM-2011 and NM-2006. The maximum plant height (52.67 cm), pod length (11.39 cm), pods/plant (22.11), seed yield (1300.22 kg ha-1) and biological yield (4439.56 kg ha-1) was recorded in crop sown on 25th April and minimum plant height (43.67 cm), pod length (9.92 cm), pods/plant (18.33), seed...

Khan Tamoor1*, Hafsa1 and Hanif Maryam2

...ria multiflora, Achillea santolina and Nepeta cateria against Meloidogyne incognita at banana orchards of District Lasbela, Balochistan, Pakistan. Crude extracts, essential oils and fixed oil of these selected plants were tested separately. The crude extracts of Z. multiflora and F. oopoda showed higher mortality as compared to A. santolina and N. cateria. The results showed a maximum mortality of 76% by Z. multiflora and F....
Bin Liu1,2, Libo Wang2, Dandan Xue2, Peng Xu1, Yuting An2 and Changhu Lu1*
...ature birds was much the same as adults of the same sex in both seasons (Mann-Whitney U test, spring, P=0.571; autumn, P=0.236). Body size and sex were found to affect body mass significantly (ANCOVA, wing length P =0.001; sex P=0.001). No individuals were recaptured in both migration seasons, hence the Blue-and-white Flycatcher may adopt a strategy of one-day stopovers. The Yancheng seawal...
Sajid Ali1, Muhammad Avais1*, Aneela Zameer Durrani1, Kamran Ashraf2, Saira Jabeen1, Sajid Hameed3, Muhammad Awais1, Jawaria Ali Khan1 and Imtiaz Ahmad4 
...s, which results in colossal economic losses for dairy producers. Nevertheless, it is overlooked and least studied dairy problem in Pakistan. The objective of this study was to determine the prevalence and associated risk factors of lameness at commercial dairy herds in Punjab, Pakistan. The sample size was 2,555 cows from 15 dairy herds assessed using a 5-point locomotion rating scale. A cow with a locomotion score of 3 or ...

Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*

...nd shrimp farming. Water samples (n = 3 each) were collected from these farms and analyzed for contents of ammonia, alkalinity, temperature, nitrate, salinity, nitrite, pH, total hardness, dissolved oxygen (DO), chlorophyll-a and phytoplankton-density during the period from March, 2015 to June, 2016. The chlorophyll-a concentration was determined by using the acetone extraction technique. Ponds water

Mian Muhammad Arif* and Malik Muhammad Shafi

...htunkhwa. A total of 105 sample broiler poultry farms were selected through random sampling technique and data were collected for 483 flocks by means of interview schedule. Descriptive statistics was used for general analysis, while budgetary technique was used for profitability analysis. Ordinary Leased Square (OLS) technique was employed to find the effect of different inputs on profit of broiler poultry farms. The ANOVA t...

Fazal Subhan1,2, Abdul Malik1, Zia Ul Haq1 and Tariq Mahmood Khalil1,3*

...es to enhance WUE and to save water without a significant reduction in the yield of cauliflower.

...

Rabia Iqbal and Muhammad Naeem *

...s were maintained within safe limits for fish growth. Ten fish specimens from each treatment were randomly collected at the end of trial for growth parameters analysis. Different growth parameters, such as mean weight gain, mean length gain, specific growth rate, protein efficiency ratio, feed conversion ratio and production were determined. Results revealed maximum gains in T3 (25%) feed group as compared to T1 (15%) and T2 (20%) feed groups. The lowest food ...

Sajid Ali, Shahen Shah* and Muhammad Arif

...ar application of full dosage at the tillering stage, full dosage at the booting stage and their respective half dosages at the tillering stage + half at the booting stage) along with one unsprayed check were used in the experiment. All the treatments’ combinations and a control {(4 x 4 x 3) +1} were laid out in a randomized complete block design having three replications. The plot s...

Muhammad Ibrahim Kubar1, Fahad Nazir Khoso1*, Imran Khatri1, Niaz Hussain Khuhro2 and Arfan Ahmed Gilal1

...eated control, T2 = Spinosad (Tracer), T3= Cue-lure, T4= Protein hydrolysate, T5 = Trybliographa daci. Furthermore, combinations of these treatments were designed to find out the most effective combination. The results revealed that among the solo treatments, the plots sprayed with chemical (T2 = Tracer) had least number of infested fruits (29.75±2.69) while in combine treatments, the most effective treatment was T16 ...

Misbah Ali1, Safdar Ali1, Muhammad Ahmad Zeshan2*, Rana Binyamin3, Nadeem Ahmed4, Muhammad Usman Ghani5 and Awais Ahmed Khan2

...um graminicola is the causal organism of red leaf spot of sorghum which causes heavy losses to the sorghum crop cultivated in different areas of Pakistan. It produces black anthracnose spots on all the above ground parts especially leaves. In the present study, four fungicides (Aliette, Cabrio Top, Nativo, and Melody Duo) were evaluated against C. graminicola under in-vitro and in-vivo conditions. The poisoned food technique was used for in vitro trials with t...

Weenghar Ali Chandio1, Tajnees Pirzada1*, Abdul Majid2 and Farzana Rashid3 

...ally extracted from soil sample of agriculture lands of Khairpur, Sindh, Pakistan by International Humic Substance Society method and confirmed by UV-Visible spectroscopy and characterized by spectral features obtained by using different techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM). Finally, the effect of extracted humic acid on wheat seed germination profile including root/shoot length, ...

Mehnaz Safdar1, Urooba Pervaiz1* and Dawood Jan2

...rposively selected and a sample size of 384 women farmers were selected through multistage sampling technique. Descriptive analysis revealed that 305 women farmers were involved in rearing poultry. A 5 point Likert scale was used, showed that women farmers had various constraints out of which Pardah, mobility and lack of information source were ranked on top with Likert score value of 4.12, 4.00 and 3.94, respectively. Due t...

Shahinshah Khan1*, Aijaz Ali Khooharo1, Zaheeruddin Mirani1 and Velo Suthar2

...lying multistage cluster sampling approach. Survey data further revealed that 34% farmers produced their own seed. Regarding source of seed, dealers (96.8%) and neighboring (88.8%) farmers were observed to be the major sources of basic seed for multiplication. However, 36.8% of the farmers reported that seed companies are their exclusive source of  basic seed. Only 14% of the farmers reported that seed was purchased from public sector. Major seed sources ...

Barış Bayraklı

... crab (Eriphia verrucosa) were determined. From the southern coast of the Black Sea, fifty individuals of warty crab were caught every month. Amino acid score (AAS), chemical score (CS), and meal size amino acid score (MSAAS) were calculated. In August, when E. verrucosa breeds densely, the crude protein (%13.52) rate was found to be the lowest compared to other months. In September, the crude fat ra...
Shuang Yang1,2, Huiting Zhao3, Xuewen Zhang2, Kai Xu1, Lina Guo1, Yali Du1 and Yusuo Jiang1,*
...xpressed between the two samples. In conclusion, the study lay a foundation for understanding the olfactory functions of chemosensory genes in G. mellonella, which can help to control and prevent the damage caused by this pest.
...

Muhammad Qaddafi Khan Wali, Arif Alam* and Ikram Shah

...an all-out number of 351 sampled households were reached through a simple random sampling strategy using a structured questionnaire as a tool for collecting data and used for various factors responsible for food security status and the affect they have on food security at the household level. We used binary regression analysis to identify factors affecting food security in the target area. This investigation discloses that 9...

Muhammad Tariq Mahmood1*, Muhmmad Akhtar2, Kaiser Latif Cheema2, Mushtaq Ahmad1, Muhammad Jahanzaib Khalid2, Afzal Zahid2, Muhammad Kashif3 and Zeshan Ali4

...iculture and Biology, Faisalabad) were most discriminating environments for grain yield and most useful for screening of genotypes. Result for yield stability indicated that G-10 (K-15019), G-2 (CH47/12), G-12 (K-15001), G-15 (CH66/10) and G-16 (CH53/12) were higher yielding and more stable across environments, therefore these genotypes may be utilized in chickpea breeding program for development of widely adapted chickpea cultivars. Moreover, G-10 (K-15019) i...

Amar Iqbal Saqib, Khalil Ahmed*, Muhammad Khalid Bhatti, Ghulam Qadir, Muhammad Qaisar Nawaz, Muhammad Ashfaq Anjum, Abdul Rasul Naseem, Aftab Ahmad Sheikh and Belqees Akhter

...: (A) Types of soils. 1) sandy loam, 2) clay loam, (B) Remedial strategies, 1). canal water, 2) saline-sodic tube well water (continuous), 3) three irrigations of tube well water and two of canal water in a cyclic manner (short cyclic use), 4) tube well water + gypsum @ RSC of water, 5) tube well water+ canal water in 1:1 ratio (conjunctive use). Data showed that the highest biomass and paddy/grain yield were produced by can...

Ayaz Ahmed* and Muhammad Zulfiqar

...villages, located in the same forest-ecological and socio-economic settings. The study analyzed the effects of JFM on local livelihood by using binary logistic regression. Odds ratio analysis of the social and natural assets data showed that the Joint Forest Management approach had significantly increased the social and natural assets of the participants. In terms of financial asset, odds ratio in favor of ‘NTFPs income’ and ‘employment oppor...

Tahseen Ullah* and Noorul Amin

...ard wood cutting). The basal ends of the cutting were immersed in the desired strength of the IBA. After treating the cuttings with solution they were planted in raised beds and covered with plastic sheets to maintain the humidity in the experiment plots. Types of stem cutting and IBA concentration have significantly affected (at P≤0.05) the sprouting %, survival %, sprout weight, dry leaves weight, leaf area, root diameter, chlorophyll content, sprout diam...

Muhammad Sarfraz1*, Muhammad Amjad Qureshi2, Muhammad Arshad Ullah3, Shahzada Munawar Mehdi4, Abdul Rasool Naseem1, Sarfraz Hussain1, Muhammad Qaisar Nawaz1 and Muhammad Rizwan1

...ustify;">Amelioration of salt prone areas and reuse of saline/ sodic water is becoming important tool to improve the crop production as well as to decrease the disposal of such water. More land can be brought into cultivation by using poor quality ground water. Poor quality water can be used for cultivation of crops with appropriate management viz. chemicals like gypsum, H2SO4, calcium chl...

Gulnaz Parveen1*, Naila Mukhtar2, Shumaila Irum3 and Nain Bukhari4

...identification of the causal pathogen is important before appropriate treatment can be made to control the pathogens. Therefore, In the present study, Alternaria alternata, Alternaria, Rhizopus, Mucor, Aspergillus niger, Aspergillus flavus, Cladosporium, Botrytis cinerea, Fusarium moniliforme, Drechslera, Penicillium, and Geotrichum candidum were identified among various vegetables including potato, carrot, okra, eggplant, t...

Samra Aftab1, Saleem Ullah1 and Farida Anjum2*

...d related constituent of saw dust and sugarcane bagasse showed that cellulose (49 %), hemicelluloses (23.5 %) and ash (1.8 %) were higher in sugarcane bagasse, while lignin (7.9 %) was higher in saw dust. The data regarding microbe isolation showed highest colonies of Aspergillus sp. (8 CFU × 104g-1) followed by Penicillium sp. and Trichoderma sp. (7 CFU × 104g-1 and 5 CFU × 104g-1), while the lowest coloni...

Hussan Ara Begum1, Muhammad Hamayun1, Noor Shad1, Waqar Khan3, Jawad Ahmad1, Muhammad Ezaz Hasan Khan2, David Aaron Jones2 and Kishwar Ali2*

...assica rapa L. and Eruca sativa L. Some seeds of Brassica rapa L. and Eruca sativa L. were placed in petri plates, and were exposed to UV light for 30, 60 and 120 mins daily. The source of UV light was a UV box having a UV Tube. The UV exposure on the seeds reduced the germination percentage in both species. The germination percentage in control was recorded as 19.35%, while it was 18.65% in 30min and 19.00% in 120min exposu...

Abdul Hameed1*, Ihtsham Ul Haq Padda1 and Abdul Salam2

...Pakistan and compare the same across various provinces. It also tracks the pattern and levels of consumption of various macro and micronutrients. The study indicates 38% of the households at the national level experience calorie deficiency (less than recommended calories). The corresponding figures for the urban and rural areas are 40.5% and 36.7%, respectively. Households experiencing deficiency in caloric intake in Balochistan and Sindh provinces are compara...

Saddar Faheem1,2, Hameeda Kalhoro1, Naeem Tariq Narejo1*, Muhammad Hanif Chandio3, Memon Samina4, Shahnaz Rashid5 and Ghulam Abbas5*

...o, Sindh, Pakistan. Fish samples (100 specimens for each sex) were collected during two seasons i.e., summer: March to May, 2017 and winter: November, 2017 to January, 2018. Haematological characteristics of the fish specimens were considered season and sex wise separately. Results showed that erythrocytes (2.09 × 106 to 2.26 × 106), leucocytes (164.90 × 103 to145.55 × 103) and haemoglobin (10.34 g/100 ml to 7.06 g/100 ml) concentration...
Shafaq Fatima1,*, Farkhanda Manzoor1, Humaira Amman1, Zakia Kanwal1, Asma Latif1, Zeeshan Ali2, Hamid Iqbal Gondal2, Sumera Sajjad1 and Raja Shahnawaz Janjua3
...lant source of n-3 polyunsaturated alpha-linolenic acid (ALA) which can be used as supplemental dietary lipid in fish feed particularly carps. In developing countries where fish oil is not an ingredient of fish feed due to its high cost, the addition of economical plant oils rich in n-3 PUFA in fish diet can remarkably improve its quality. The present study evaluated the effect of the addition of linseed in fish feed on growth and fatty acids profile in grass ...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
...trol (C) and offered a basal diet. The chicks were reared in floor pens bedded with saw dust litter. Measured feed was offered twice a day and drinking water was offered ad-libitum. Growth performance parameters, hematology, serum biochemistry, immune response (ND and SRBC), intestinal histo-morphology, bacterial enumeration and cecal volatile fatty acids (VFAs) were measured. There were significant improvements in we...
Muhammad Mohsin1, Yin Hengbin2*, Qian Chong3 and Bohyun Kim4
... regime, it becomes necessary to estimate overexploitation risk and understand ongoing economic implications of particularly important fishery resources. Thus, this study is the first-time attempt to evaluate very important fishery resource, i.e., Penaeus shrimps of Pakistan by considering these aspects. A long catch and effort (CE) data series, 1971-2009, were used in this study. CE data was statistically analyzed by using non-equilibrium surplus produ...
Asif Sadam*, Rahmat Ullah Khan and Sajid Mahmood
...gricultural area. Twelve sampling sites were selected in well-defined habitats in Mardan district. Bird species traits were taken from field observations and published literature. The trend and variation in traits was recorded along a sampled gradient of decreasing urbanization from urban area to agricultural area within district Mardan. These traits were compared among urban exploiters, adopters and avoiders. Thirty-five sp...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...ated. Cell sensitivity assay (MTT) showed that golden piller was more toxic than galangal in cell viability tests and more potent in the cell growth inhibition. The calculated LC50 were 120 µM and 200 µM for golden piller and galangal, respectively. Both plant extracts significantly reduced the gene expression levels of chitinase genes and SFbtl (p<0.05) following 72h of exposure to LC50. This work provides evidence of a vi...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...n.A total of 250 poultry samples, 92 poultry environmental samples, and 50 poultry farm worker’s urine samples were screened for ESBL producing E. coli using ChromeIDTM ESBL agar. Biochemical confirmation of the isolates was carried out by API 20E. The antimicrobial susceptibility profiling and phenotypic confirmation of ESBL producers were performed as per CLSI 201...
Zeynep Sonmez1 and Memis Ozdemir2,*
...72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in the determined genes and gene regions as a result of the sequence analysis. In the 1st exon of the EPS8 gene, 9 polymorphic regions were identified as g.94554132C>T, g.94554252G>C, g.94554348T>G, g.9...
Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*
...riological analysis of nasal swabs of 52 diseased and healthy donkeys at different localities in Egypt revealed that the overall occurrence of Cryptococcus spp. was 11.5%. The highest proportion was in El-Fayoum Governorate (25). Phenotypic identification of Cryptococcus indicated that 13.2 % and 7.1% among healthy and diseased donkeys were positive for this pathogen, respectively.The study of the potential risk factors associated with Cryptoc...
Kai Huang1,2, Wancai Xia1,2, Yi Fu3, Yaqiong Wan4, Hao Feng2, Ali Krzton5, Jiaqi Li4,* and Dayong Li1,2,*
...emale individuals in the same one-male unit (OMU), and the grooming reciprocity index was used to detect reciprocal relationships between these females within the OMU. The results showed that loose social hierarchies exist among the females in each OMU, and more dominant individuals have higher grooming incomes. These results are consistent with the aggressive-submissive hypothesis and the resource control hypothesis.
...
Arshad Ali1, Muhammad Nasir Khan Khattak2*, Muhammad Ali Nawaz3 and Shoaib Hameed3
...n bear (Ursus arctos isabellinus)), grey wolf (Canis lupus), and lynx (Lynx lynx) often encounter humans and contribute to significant economic losses. The present study was conducted in Kalam Valley, District Swat, Pakistan to estimate the occurrence of brown bear and conflicts with humans. Data about the presence-absence and depredation in five years (2009-2013) were collected from 86 households in the area. Total losses in Kalam Valley ...
Muhammad Ramzan1*, Unsar Naeem-Ullah2, Muhammad Umair Sial2, Naeem Iqbal2 and Shafqat Saeed2
... justify;">Ficus spp. (Rosales: Moraceae) are a large group of plants grown for landscape and medicinal purposes in various regions and also known for absorbing various toxic pollutants from the environment. Several insect pests like thrips, whiteflies, mealy bugs and caterpillars attack on these plants. Among them, leaf eating caterpillar, Trilocha varians (Lepidoptera: Bombycidae) is serious insect pest for these precious plant species in various countries. ...

Ahmed A. Kheder

...ing 2018-2020. Forty-one samples out of 693(5.94%) reacted positively to SLRSV. Specifically, the percentages of infection were 7.3, 6.7, 5.4, 5.16 and 2.63% in El-Qalyubia, El-Beheira, El- Menoufia, El-Sharkia and El-Ismailia governorates respectively. SLRSV was mechanically transmitted from infected strawberry plants onto ten herbaceous plant species, different types of symptoms have been observed associated to virus infection on indicator hosts; Mentha spic...

Mohammed Bashar Danlami1*, Basiru Aliyu1, Abbas Bazata Yusuf1, Bashar Gulumbe Haruna1 and Asmau Nono Mahe1

...alence of abnormal and unsatisfactory Pap smear among women attending Federal Medical Center Birnin Kebbi. The study was a retrospective review of all Conventional Papanicolaou smear registry data of women who underwent a cytological examination at the cytology unit of the FMC Birnin Kebbi spanning a period of 10 years (2009-2019). The study used a hospital-based archive that covers the entire Kebbi State without any well-defined population to collects data on...

Sehrish Khudadad1, Muhammad Ather Rafi2, Ahmed Zia3, Mian Sayed Khan4, Gulnaz Parveen5, Muhammad Kamal Sheikh6*, Falak Naz7, Muhammad Qasim8 and Syed Waqar Shah9

... pollinators, seed dispersal/ orchid seed removal agent and scavengers. Ants can be both prey and predators for the crops and various insects. They also spread human diseases. Ant fauna is poorly known in Pakistan. As Pakistan occupies an important biogeographic position and the taxonomic studies on ants of Pakistan are badly needed. During this study ant specimens were collected from different localities of district Mansehra. Total 28 species were identified ...

Muhammad Shahzad1*, Syed Amer Mahmood2, Athar Ashraf3, Amer Masood2 and Saira Batool4

Iftikhar Jan* and Sahib Alam

...l and stored maize grain samples collected from four agroecological zones (AEZ) i.e., AEZ-A, AEZ-B, AEZ-C and AEZ-D of Khyber Pakhtunkhwa (KP) province of Pakistan. The mycological analysis of the samples demonstrated that the highest population of Aspergillus section Flavi was existed in soil (n = 236) than in maize grain samples (n = 198). Across all the four zones, the highest populatio...

Syed Muhammad Sulaiman, Nazir Ahmad and Nazir Ahmad Khan*

... and quality evaluation, samples were collected from randomly selected one-meter long strip of two consecutive rows of each plot at feekes stages 7 to 8, when the second last leaf was visible. The collected samples were immediately weighed, and 10 plants were subsampled from each sample for morphological evaluation. Ten plants were weighed and separated ...

Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1

...tablished, so it is necessary to produce its active compounds abundantly and to propagate purple and white-purple varieties of O. aristatus. A strategy that can be adopted  to achieve plant tissue culture (callus induction). This research aim is to identify secondary metabolite in callus using thin layer chromatography (TLC). The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl aceta...

Shahzad Khan1*, Munir Khan1, Arif Alam2, Ikram Shah2, Mahfooz Khan1 and Fida Muhammad Khan1

...eat crop in district Charsadda, Khyber Pakhtunkhwa, Pakistan in the year 2018. Three villages, namely, Aspandehri, Kamran Kalay and Sarfaraz Kalay, were purposively selected. A sample size of 41 wheat growers from farm households was chosen from these villages for data collection. Thus, selection of the farmers was based on the proportional allocation technique. However, primary data were randomly collected through face to f...
Samina Tahir Kiani1, Abdul Rauf1, Syed Ayaz Kazmi1,*, Nuzhat Shafi1, Madiha Khalid2, Naeem Latif1, Samina Gul2, Saghir Yousaf3 and Muhammad Arshad4
...Jammu and Kashmir. Blood samples of a total of 300 children (150 males and 150 females), 4-17 years of age were screened for anti-HCV antibody, HBsAg and anti-MTB antibodies using ICT kits. Sputum microscopy for MTB was done in MTB antibody positive samples. The samples positive in initial screening were confirmed by enzyme linked immunosorbent assay (EL...
Ying Yang 1,2,3, Tietao Zhang 1,2,3, Min Rong 1,2,3, JiaPing Xu 1,2,3 and Xiumei Xing 1,2,3*
....06 KJ/kg appeared to be satisfactory for the growth of female.
...

 Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*

...d flies.
The salivary gland symptom was similar to the previously reported
symptoms of the MdSGHV. Examination of the SGH suspension with
Electron microscopy revealed the presence of rod-shape virus particles
with 500-700 nm in length and 50-70 nm in diameter. PCR analysis of
SGH homogenate with virus specific primer pair resulted in DNA
fragment with expected ...

Ain-ul-Abad Syed1*, Zaheer Ahmed Khan1, Shakeel Hussain Chattha1, Irfan Ahmed Shaikh2, Mian Noor Hussain Asghar Ali1, Zohaib ur Rehman Bughio3, Shahzad Hussain Dahri2 and Ghous Bakhsh Buriro4

...ivation is successful in saving water and fertilizer and increasing crop yields at the desirable limit. To enhance yield, the hydroponic model should be equipped with sensors and artificial intelligence technologies to handle difficulties related with food supplies. The hydroponic growing technology should be introduced to cities. At the time, such as when challenged with Covid-19 and the locust attack.

...

Ghulam Murtaza1*, Muhammad Ramzan2, Assad Ullah3, Abid Ali5, Ayesha Zafar3, Rukhsar Beanish3, Ahmad Ali4, Ghulam Mustafa4 and Mudassar Aslam4 

...e placed in cages having sand in bottom for pupation. After the adult emergence fruit fly species were identified based on diagnostic morphological features and placed in separate cages. Different fruits such as guava, apple, fig, banana and citrus were exposed to Bactrocera zonata for oviposition. Fruits were placed in cage to assess the host preference for oviposition fruits having equal weight (500g). Fifty pairs of 11-12 days old adult of B. zonata were re...

Ahmed F. Afify1, Mohamed A. Shalaby2, Ahmed A. El-Sanousi2 and Amal S. Gaber1

...total of
548 samples were collected from governmental and private studs of Arabian horses
and also foreign horse breeds, including 540 serum samples, among these, 4 EDTAblood
and 4 semen samples. Serologically, indirect ELISA was performed on 540
serum

NawalM. Abdulla1, M. Haroun1*,Mohamed A. Shalaby2and Ahmed A. Elsanousi2

...entic analysis using the same primers.
...

El-Habbaa, A.S.

...wide and in Egypt. Forty samples of skin lesions from suspected 30 sheep
and 10 goats from different areas of Qaliubeya governorate were subjected
tovirusisolation on embryonated chicken eggs (ECE), antigen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were show...

Walaa ,S. Shabanaa;Bakr, A. Abdallaa; Eman, M. ELShalamia; I.M. Redab; M. A. Shalabyb

Ala’audin Hakami,1AusamaA Yousif,2* Mohamed Zaki,3 Mahmoud Ismail,4,5 Ahmed Al-Ali,5 Abdu-Rahman Al-Ankari5 
...
feather and blood samples from randomly selected 19 clinically-suspect
and 153 apparently-normal birds collected from 10 locally-bred and
imported Psittaciformes. Specimens were collected between 2008 and
2010. A replicase-associated protein (Rep) gene-specific PCR was used to
amplify a 603 bp region of the viral genome. PBFDV was detected in
31.6% of clinical cases (3.5% of
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...ts demonstrated that the safest
concentration (non-cytotoxic) of the plant extract under study on MDBK cells was 0.1
mg/ml.The plan extract showed a significant antiviral effect against BHV-1 at
simultaneous treatment experiment. The virus titeration assay showed a marked decrease
from 105 TCID50/ml to 103 TCID50/ml in adsorption step simultaneouslly incubated
with ...
El-Dougdoug K.A.1, Sofy A.R.2, Rehab A. Dawoud3 and Korkar H.M.4
...echniques. Osmotherapy wasapplied for
eliminationbanana bunchy top virus(BBTV) through preservation of shoot tips on
MS media containing high sucrose concentration at low temperature. Shoot tips and
subculture 1st of banana plantlets were preservedsuccessfully for 12 months on
storage MS medium supplemented with 40, 50, 60 and70 gL-1 at 10, 15 and 20ᴼC.
The highest survival percentage 100% with reco...
Mandour, A. M. 1, Abdel Maksoud, H. M. 2, Amal, A. Khalil 3 and Mohga, A. El-Tahlawey 4
...so rested. Maize dwarf mosaic virus (MDMV) is the most
economically significant virus infecting maize crop in Egypt. The percentage
of infection differs from one location to another. MDMV was isolated from
naturally infected maize plants at El-Monfia. These samples were checked
for virus presence using Indirect ELISA<...
Mansour, L. L.*; Othman, B. A. **; Abd-EL Ghaffar, M. H. **; Eman, M. Marai** and Sahar, A. Youssef* 
...d from infected banana(Musa
acuminata cv Grandnain)obtained from EL-Behira, Egypt
byPentalonianigronervosa .Polymerase chain reaction (PCR) was used to detect
BBTV in infected plants and banana aphid (P.nigronervosa) using specific primer
of DNA-1 for BBTV. The results showed that amplified PCR product with the
expected size 476 b...
Sallam A.A.A.1, Hoda M.A.Waziri2,E. K .F. Elbeshehy1, SamiaI.Massoud,1 and Abeer M. Abo El-Wafa2.
Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1
...lost its ability to lyse salmonella cells at pH 4, 5, 6, 10, 11 and 12
while it was infectious only at pH 7, 8 and 9.The virus lost its infectivity after
exposure to UV for 50 min. at distance of 53 and 70 cm from the UV source. The
bacteriophage (St-1) was inactivated in presence of NaCl at concentration higher
than 15%; sodium benzoate at concentration of 0.5%; potassium sorbate at
concentration of ...

D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3

...nvironmental or clinical samples. Its replication in cell culture is slow and so cytopathic effect might take up to two weeks to appear making it not easy to detect presence of virus. the present work aimed to study effect of inhibiting HepG2 cellular proteases on replication rate of the virus. Plaque infectivity count assay was carried out using safe concentrations of the cocktail proteas...

Maha I. El Zaafarany1, WafaaM. Badawy2, Eman M. El Salakh3

...sh frozen breast cancers samples were analyzed. Samples were tested for HPV by PCR, and products were sequenced. Findings were correlated with clinical and pathological characteristics.The HPV DNA prevalence in the breast cancer samples was 28 % (15/54). Sequence analysis in a subgroup of cases indicates the prevalence of high risk HPV16. In conclusion, the present study demonstrated for the presence of HPV in Egypt in a pro...

Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.

... superoxide dis-mutase assay kit. Genotyping was performed by polymerase chain reaction and re-striction fragment length polymorphism (PCR-RFLP) method. The homozygous Ala/Ala (CC) was significantly more frequent in HCV-related HCC patients com-pared to HCV-infected patients and control group (67.5%, 12%, and 15% respective-ly, p ˂ 0.05). Moreover, the C allele was more often associated with HCV-related HCC patients compared to HCV-infected patients and contr...

Enas K. Abo-Elmagd,Kouka S. Abd El-Wahab,Azza H. El- Salakawy

... immuno-chromatography assay (LFICA) as a rapid test for detection of RV antigen in stool specimens collected from Egyptian infants and young children with ELISA, and nested reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequency of RV infection in Egyptian infants and young children during the summer season 2012 and the effect of certain risk factors ...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...ted from cattle clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID and 3D genes were determined using standard automated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) revealed (99–1...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...luenza (HPAI) H5N1 virusesare now endemic in Egypt inducing economic losses in poultry industry and subsequent risk of human infection. Vaccination is an effective method for controlling avian influenza virus infections.This study was conducted to evaluate the efficacy of the commercially applied AI vaccine regimens against challenge with three H5N1 HPAI viruses isolated from chickens, Egypt during 2009-2011. Both inactivated and recombinant fowlpox virus expr...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

...hicken eggs for three passages followed by ten successive passages in Madin Darby Bovine Kidney (MDBK) and identification of isolated virus was done by indirect immunofluorescence test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves a...

Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

...tavirus antigen in fecal samples of forty diarrheic new borne calves of cattle (32) and buffaloes (8) below three months in some farms in Egypt. The detection was done by ELISA and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal ...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...resent study, twenty one samples in the form of nodular lesion of skin were collected at different stages during the course of the disease from private farms in Ismailia and Fayoum governorates during April to June 2013. Different diagnostic tests used for Isolation of LSD virus from the nodular samples by inoculation on chorioallantoic membrane (CAM) of embryonated chicken eggs (ECE) SPF at 9-11 day and Madin Darby bovine k...

Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3

...rmed directly from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 98.4-98 and 98.4% respectively. Egy/cairo/14 share nucleotide identity with the vaccinal strain flury LEP and kelev with a percentage of (93.1% and 98% respectively). All Egyptian...

Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***

Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3

...t study, thirty tracheal samples of broiler flocks suspected to be infected with IBV were collected from different Egyptian governorates during 2012. Isolation and genetic characterizations were carried out for only ten samples which were positive for IBVs. Multiple nucleotide and amino acids alignments revealed prominent nucleotide substitutions which affect amino acids composition of HVR3 of S1 subunit. Phylogenetic analys...

1Mona A. El-Manzalawy, 2Abeer A. Boseila , 3Hanan M. El Zahed

...lum gel, then ensure the safety and sterility of these vaccines and compare between them with the local RVF vaccine with Alum gel alone in sheep. This peppermint formulation including Alum gel adjuvant was the best of choice as it induced higher immunological enhancement. In addition, synergistic action was discovered in RVF inactivated PMO vaccine including Alum gel as the immune response detected by serum neutralization test (SNT). This vaccinated group of s...

Hala K. Abdelmegeed 1, Eman M. Abohatab 1, Khattab,O. M. 1 , Salem, S.A.H. 1 , Arafa, A. 2, Nashwa M. Helmy 3

...udy 161 out of 376 serum samples in percentage 42.8% were positive for FMD non structure proteins( NSP) antibodies which indicated the natural infection. ELISA kit used for FMDV antigen detection for the epithelial suspension from 42 field samples collected from various geographic locations 10 governorates are ( El-Sharkia,Giza, Port Said, Assuit, Suize,...

Nashwa M. Helmy1 and Ahmed S. A.2

...and Indirect
sandwich ELISA in suspected samples. The serum samples were collected from Sharkia
and Fayoum governorates (30 and 40 sera respectively) submitted to laboratory
examination by Priochek for NSPs (3ABC) 32 out of 70 were positive (12 sera sa...

El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.

...aloes. Twenty Buffy coat samples were collected from each clinically infected cattle and buffaloes in Qaluobia province during the year 2014. The virus was isolated on BHK-21 cell line. Infected cells with BEFV showed 80% cytopathic effect (CPE) after 48 h by the 3rd virus passage. The isolates were serologically identified by neutralization and immunoflourescence techniques, and then molecularly identified using reverse tra...

Nelson D. Zambrano1, Wilber Arteaga1, José Velasquez2 and Dorys T. Chirinos1*

...illa maculata, Cycloneda sanguinea, Cheilomenes sexmaculata and Zelus sp., were inferior in the treated plots. Yield ranged from 1.2 to 1.4 tons. Thrips palmi is first reported feeding on cotton in Ecuador. The results showed high populations of B. tabaci in the chemical treatment, and its suppressive effect on the beneficial entomofauna. Future research should focus on evaluating the selective applications of some insecticides to control A. gossypii and Dysde...

Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2

...e HSV-2 gD antigen was assayed by ELISA on protein extracts isolated from same transfected plant tissue samples as for the RT-PCR assays. Results showed that the HSV-gD2 construct was expressed at both the RNA and protein levels in the transgenic tomato plants. Finally, we report her...
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H.
M. Mazyad1
...iv>...

Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4

...ctrum of purified Beet mosaic virus (BtMV) particles obtained by density gradient centrifugation revealed that, the concentration of virus preparation estimated using spectrophotometric measurements at 260 nm was 3.004 mg/ml. The min, max nm, Amax./Amin., A260/A280 and A280/A260 ratios were 244, 260, 1.276, 1.512 and 0.662, respectively for BtMV. The corresponding figures of the purified virus preparation of Beet necrotic yellow vein virus (BNYVV) were 2.196 m...

Hanaa H. A. Gomaa1; Entsar A. A. Nassar2; K. A. El-Dougdoug3

...marin production has the same pattern of fruits yield. The methanolic solutions were extracted from milk thistle varieties then evaluated against HAV, HSV1 and COXB4 viruses. The cytotoxicity of different milk thistle methanolic extracts in Vero cells did not show any morphological difference compared with control. Antiviral affects of milk the most potent methanolic solutions of milk thistle that have antiviral activity with different degrees, line 32 with (H...

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

...resistant (Diamond, Execusa, Hermes and Valor), tolerant
(Sisi, Lady Balfor, Nikola, Ditta and Lady Rosetta), susceptible (Anabel and Spunta) based on
different external symptoms, the severity infection and virus concentration. Expressed protein
was differed in leaves of potato cultivars. Since PVY infection unexpectedly increased in
(Anabel, Sisi, Lady Balfor, Nicola and Execusa

Om-Hashem M. El-Banna1, Maisa A.E. Awad2, M.S. Abbas3, Hoda M. A. Waziri2 and Huda S. A. Darwish2

...checked in the suspected samples by ELISA using the specific antiserum. Anatomical and ultrastructure changes in leaf tissues of both Tomato and Grapevine artificially infected with Tomato ringspot virus were studied by light and electron microscopy. By light microscopy viral infection resulted in the presence of amorphous inclusion bodies in the cytoplasm of infected leaves.Phloem tissues...
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H. M. Mazyad 1 
... Egypt. Symptomatic leaf samples were collected from bean fields
cultivated in different governorates and tested by PCR using Geminivirus degenerate
primers and Squash Leaf Curl Virus (SLCV) specific primers. All bean varieties grown in
surveyed governorates were found susceptible to Geminivirus infection and the dominant
Geminivirus affecting bean fields was SLCV. Percentage of infection was higher at Nili
...
M. A. S. El-kady1; A. B. Badr2; Ahmed K. El-Attar1, Hoda M. A. Waziri1 and Kh. E. A. Saker1
Aly M. Abdel-Salam1, Rehab A. Dawoud2, Amira M.E.Aly2, and Salama M. El-Saghir2
...dish subgroup) banana (Musa acuminate)
variety and from Pradica (AAB group) banana (Musa × paradisiaca) variety,
collected from different locations in Egypt, were tested for the presence of Banana
streak virus (BSV) using immunocapture-polymerase chain reaction (IC-PCR) and a
specific antiserum for BSV. IC-PCR indicated the episomal presence of BSV in
Williams...

Mohamed I. Azzam1, Safaa M. Ezzat1, K. A. El-Dougdoug2, Badawi A. Othman2

...iv>
drainage water samples. Transmission Electron microscopy revealed that, the isolated
coliphages have an isometric head and long-contractile tail; some particles revealed a
short tail with full heads, resembling those belong to the myoviridae and siphoviridae
family. Restriction enzymes by EcoRI, HindIII and BamHI showed the presence of
double stranded (ds) DNA as well as heterogeneity among these phages...

Rehab A. Dawood1; Entsar A. Nassar2; and K.A. El-Dougdoug 3

... stem nodes at MS medium saturated with sucrose. Stem nodes of potato were successfully stored for up to 12 month at osmo-preservation medium supplemented with sucrose 50, 60 and 70 gl -1 at 210C. The survival percentage was recorded 85.1, 81.2 and 50.8 for 50, 60 and70 gl -1 respectively for 12 months at 210C. All survival plantlets revealed viability and resumed growth on fresh medium within three weeks. PVY was eliminated from infected potato plants using o...

Eman A. H. Kattab1,3, A. H. Ebrahem2 , Om-Hashem M. El-Banna2 and Hanan F. EL-Kammar³

Amel, S.M. Abo-Senna1; M.A. Nasr-Eldin2; B.A. Othman3 and A.A. Megahed4

...

...

Aly M. Abdel-Salam

...ts vector Saccharicoccus sacchari and in another unidentified
Pseudococcidae- mealybug. Amplicons for the RT/RNaseH motifs of the two virus
isolates were cloned, sequenced, and submitted to the GenBanak. Pair-wise nucleotide
identity indicated 86% identity between the Giza and Assiut isolates. NCBI-blastn
analysis for the two isolates with four SCBV accessions retrieved from the GenBank
indicated iden...

Amira A. Mazyad1; A. A. Kheder1; Ahmed K. El-Attar1; W. Amer.2; Mahasen, H. Ismail.2; Amal, A.F.1

...nt>) and molecular assays. Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify 497 bp fragment using PCR primers specific for the viral coat protein gene as a tool for molecular diagnosis. The PCR detection was confirmed with direct DNA sequencing and phylogenetic analysis for the coat protein gene. Further insurance of SLRSV infection was performed using light microscopy which showed presence of amorphous inclusion bodies, electro...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...v>
large number of samples and for isolation of RNA and DNA from
small quantities of tissues. The method does not require expensive
and environmentally hazardous reagents and equipment. It can be
performed even in low technology laboratories. The amount of tissue
required by this method is ∼50–100 mg. The quantity and the quality
of the nucleic acid extracted by this method is high eno...
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1 
...
positively when assayed by nested polymerase chain reactions (PCR) using universal
phytoplasma-specific primer pairs P1/P7 and R16F2n/R16R2. Similar assays were used
to detect phytoplasma interactions with experimentally host plant. Dienes’ stain was also
used for detection of natural infection of phytoplasma. The phloem of infected tissues<...

Samah A. Mokbel, Soad H. Taha, Mahmoud M. Abd-El Hamid, Ali H. Hamed

AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1

...isease symptoms and bioassay experiment confirmed virus concentration
response mortality. Injection of a purified virus suspension to third instar larvae of Galleria
melonella and Spodopteralittoralis failed to induce any mortality indicating that the virus is
most likely host specific to N. viridula. This virus is similar to the previously reported NVV-1
virus in N. viridula from South Africa strongly suggesting...

Aly M. Abdel-Salam1 and Samah A. Mokbel2

...lotting immuno-binding assay (DBIA) showed positive reaction between
NRSV-infected apple sap and an Egyptian antiserum for NRSV. Purified preparation
from infected leaves, using the electro-elution technique yielded nucleoprotein which
had Amax and Amin at 260 and 240 nm respectively. Electron microscopy examination
showed spherical virions with ca. 26 nm in diameter.
...
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3 
... the present work, Pea mosaic (PMV) was first reported on cowpea
(Vignaunguiculata (L.) Walp).PMV was isolated from naturally infected
cowpea plants, showing mosaic and choloratic ring spot symptoms, which
had been grown in the experimental fields of Giza Agricultural Research
Experimental Station (A.R.E.S) in 2008. Identification studies based on host
range, sympto...

Arshad Abdulkhalq Yaseen1,2* and Mária Takácsné Hájos1

...ters of lettuce (Lactuca sativa L.) grown under plastic tunnel conditions. In this regard, three different lettuce varieties (May King, Kobak and Great Lakes) were grown in spring 2019 and 2020. The concentration of 6% MLE was sprayed as a biostimulant every two weeks from transplanting with the amount of 25 mL plant-1. The results indicated that lettuce vegetation quality (head structure, head weight and root weight) was significantly improved by the use of 6...

Sadiq Haladu1*, Taiwo Oladoye Akande2 and Mudassir Nasir3

...e diets were fed to 105 Isa brown laying birds with 15 birds per treatment arranged in 2x3+1 factorial. Samples of blood and egg yolk taken were analyzed for serum components, haematological indices and lipid profiles. The results of plasma lipid profile showed that diet 4 (78.76 mg/dl) is significantly low in HDL compared to other dietary treatments. Egg total cholesterol showed significant reduction in diet 5 (254.65 mg/dl) observed when compared to the cont...

Maryam Yousaf1, Salman Ahmad1*, Romana Anjum2 and Muhammad Zeeshan Majeed3

...sease caused by fungus Fusarium oxysporum f. sp. lycopersici (FOL). Two months old tomato seedlings were grown under normal conditions in green house and were artificially inoculated with FOL conidial suspension of 1×106 conidia/mL by drenching method. Untreated plants served as control. First disease symptoms appeared after seven days of inoculation and seedlings became wilted within 30 days post inoculation. Stems from wilted as well as control seedlin...

Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3

...or the negative 152 sera samples to measure the titer of
antibody against strains A,O, SAT-2 with high titer of antibody against serotype (A) was 87 ,
serotype (O) was 72 and serotype (SAT-2) was 53 . Matching between SNT and ELISA

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune sera for each virus by virus neutrization test (VNT). As the Neutrization index (NI) for PPV and FPV isolates were 2 and 2.75 when neutrized by FPV hyperimmune sera (HIS) while it was 2.5 and 2 when neutrized by PPV (HIS). DNA was extracted from the produced pock lesions and polymerase chain reaction amplifying 578b...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

... aimed to isolate the causative agent and identify it by serological characterize of the viral antigens, then molecular characterization of its genome. Infection by the bovine ephemeral fever virus (BEFV) was noticed in Dakahlyia governorates during the summer season of 2015. Twelve samples of buffy coats were collected from dairy farms suspected from clinical investigations to be infected by bovine ephemeral fever virus. Th...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...rabian Horses ,collected samples were either aborted fetal tissues either Lung and livers as well as Aborted placenta and nasal swaps from mares suffering from repeated Abortions, The current study used the gene synthesis technology introduced by Invitrogen to synthesis the glycoprotein D of the Kentucky Strain as reference strain to use it as appositive control for the envelope glycoprotein D of EHV-1 (EHV-1gD) cloned in pM...

Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1

...nd Land Reclamation, the samples collected from the stud were either aborted fetal tissues or nasal swaps from apparently healthy animals and previously aborted mares the current study focuses on isolation and molecular characterization of EHV-1 of the local isolate Egypt/VSVRI/Zahraa/2014 using the glycoprotein D of EVH-1 due to its role in virus infectivity and its function in entry of virus into cells and is considered as...

Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1

...cial one-day-old broiler saso chicks were divided into 8 groups (35 birds each). Groups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines with different adjuvants except for group 1 & 2. Group 1 was kept as unvaccinated control. At 28 days all groups were challenged with a dose of 105.5 EID50/bird of Variant 2 genotype (1494) IBV through oculo-n...

Megahed, A.A.1; Kh. A.El-Dougdoug2 and B.A. Othman2

... incidence of Cucumber mosaic cucumovirus (CMV), Beet mosaic potyvirus (BtMV), Beet necrotic yellow vein benyvirus (BNYVV), Beet curly top geminivirus (BCTV) and Beet yellows closterovirus (BYV) symptomatology and serologically by double antibody sandwaich Enzyme linked immunosorbent assay (DAS-ELISA

A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3

...e-linked immunosorbent assay (ELISA). The conjugated antisera that prepared in this study were succeeded to detect the virus in infected plants.

...

Sahar A. Youssef1; Manal A. El-Shazly1,2; Azza G.Farag1,3; Eman A. Khattab1,2

...lotting immune-binding assay using authentic and induced antiserum for PNRSV.RT-PCR with specific PNRSV primer was used to confirm the obtained results. No amplified product was obtained from healthy control. The partial RNA3 movement protein product from PNRSV infected rose directly cloned using the TA cloning system. Nucleotide sequencing revealed the clones to be portion of PNRSV genome. These results indicate that the virus in rose is an isolate of PNRSV f...

Badr, A. B. 1, Abou-zeid, A. A2. and Al-Naggar, A. M1.

...per epidermal layer, palisade and spongy tissues, length and width of protoxylem and metaxylem vessels were reduced. However, lower epidermal layer was increased. Stomatal characters i.e. number of stomata and length of stoma pore were increased, while, width of stoma pore, length and width of guard cells were reduced. Anatomical characters of tubers i.e. thickness cork layers was slightly increased, however, its number was sharp reduced compared with healthy ...

Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar

...WPV with AI vaccine were satisfactory and equal to or above that of AI control group (80-85%). It is advisable to apply vaccination with the live attenuated CAV vaccines in poultry farms to select hygienically well controlled farms or even farms with closed system which maintain their birds in strong and well healthy state. Also it is preferable to vaccinate these birds with the inactivated AI vaccine at the 2nd WPV with eit...

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and M. A. Shalaby2

...us contaminant and PCR assay for detection of bacterial contaminants as mycoplasma.

...

Serageldeen Sultan1, Nabila Osman2

...pt. A total of 510 serum samples were collected from broiler breeder (n=191), layer breeder (n=235) and commercial broiler chickens (n=84) from different farm flocks (24 flocks) in Egypt. These samples were examined for the presence of avian HEV antibody using enzyme linked immuno-sorbent assay (ELISA). The results rev...
Eman A. Ahmed1, Osama Y. shalaby2, Emad F. Dwidar2, Samah A. Mokbel1 and Ahmed K. El-Attar1
...f flower petiole. At the same time, tomato leaves were greatly affected as a result of the infection with phytoplasma. The thickness of leaf blade was remarkably increased by 200% as well as thickness of either palisade or spongy tissues by 212.5% or 275%, and significant malformation in leaflet midvein was observed which consequently led to increase in both length and width of midvein by 15.19% and 5%. Electron microscopy w...

Manal A. El-Shazly1. M. I .Kobeasy2and Sarah H . Altalhi3

... symptoms circumvented mosaic, curling, bronzing and/or purpling, chlorosis and necrotic spot on the leaves. Disease symptoms of infected fruits produced from inoculated healthy tomato seedling showing discoloration, faint concentric rings, necrotic and chlorotic spot on mature tomato fruits. The virus was biologically purified from a single local lesion formed on Chenopodium amaranticolor Caste & Reyn. The isolated virus was identified on the basis of sym...

Radwa M. Shafie 1 and Soha S. M. Mostafa2

...e, against Bean yellow mosaic virus (BYMV) infection on Vicia faba L. Giza 716 and Chenopodium amaranticolor Cost and Reyn. The non-inoculated algal synthetic media were used as control. Findings revealed that the mixture of the three algal filtrates gave the highest percentages of inhibition. Lower inhibition effect was produced by culture filtrate of N. muscorum and S. platensis, respectively in particular in plants treated with algal filtrates by mixed with...

Shahida Sabir1, Anwar Ali Shad1, Tariq Masood1*, Zafar Ali Shah1, Nasiruddin2, Yaseen Ahmad1 and Syed Sartaj Alam3 

...Aspergillus flavus and Fusarium sp.). Methanolic extract of P. eryngii showed increasing % mortality against red flour beetle (Tribolium castaneum) in methanolic extract with increasing concentration after 24 and 48 h exposure time. Maximum mortality (100%) was noticed at 300 mg/L after 40 h exposure time. Hence, methanolic extract of P. eryngii can be used as a natural pesticide, especially in stored grains. 

...

Rafia Ahsan1, Saif Ullah1, Ijaz Yaseen1, Faisal Sohail Fateh1, Muhammad Fayyaz1, Shahzad Asad1, Atif Jamal1, Muhammad Sufyan2 and Muhammad Zakria1*

...orty seven diseased leaf samples were collected from the entire surveyed locations. Thirty isolates were confirmed as Xanthomonas oryzae pv. oryzae (Xoo) through PCR and pathogenicity on susceptible host. The results indicate that BLB is continuously present in rice growing areas of Pakistan specially in Basmati area of Punjab for last many years. There is need to introduce resistant Basmati varieties to reduce the inoculum level and yield losses.

...

Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed

...nd: A total of 30 biopsy samples from cutaneous nodules were obtained from infected animals at different stages during the course of the Lumpy skin disease (LSD), and (100) Peripheral blood samples without anticoagulant were drawn from apparently healthy non vaccinated cattle against LSDV and (100) serum samples were drawn from cattle 4 weeks post vaccination with local attenuated sheep po...

Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5

...ction (PCR). Results: 15 samples were +ve for LSDV by conventional PCR.The results showed that 11/23 biopsies were positive by IFAT. Molecular identification of LSD virus by using RT-PCR, revealed positive for amplification of bands at the predicted molecular size (1926bp). Neutralizing antibodies against LSDV were (55) out of total 100 serum samples by serum neutralization test. Conclusion: Selection and processing of clini...

Lamya A. F. Ateya1 , Said. A .Ahmed2, Khamees K.S. Ashraf3, Heba A. Abdel-Hady4

...rate. Methods: 220 serum samples were tested for FMD NSP by 3ABC Trapping ELISA.20 serum samples were used as control (no vaccination),100 serum samples collected from animals received local vaccine and 100 serum samples from animals received imported vaccine. Results: 34/220(15.5%) ...

Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.

...>tests such as identity, safety, purity, potency and efficacy that were not satisfactory in vaccine
evaluation.
Objective: The present study was conducted to standardize a protocol for quality control and
evaluation of the recombinant HVT-H5 vaccine.
Methods: The protocol was designed for evaluation of H5 identity besides regular vaccine safe...

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

...tion with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 98.2% and 95.2% nucleotide similarities and 96.8% and 91.4% amino acid similarities with the commonly used IBV vaccine Massachusetts strains H120 and M41 respectively. Egypt/Qal/014p had a unique amino acid substitution at residues 39 (Serine), 40 (Tyrosine), 41 (Lysine), 64 (Gl...

Salama M. El-Saghir1, 2

...mptoms, viz. rugosity, mosaic, stunting, leaf bronzing, and early
leaf dropping. Heavy losses; however, can occur upon mixed infection with PVS and PVX or PVM.
Objective: The objective of this study is to characterize the incidence of PVS serologically with
authentic antiserum for PVS and, molecularly using specific primers for PVS.
Methods: In-direct ELISA...

Salama M. El-Saghir1, 2

...R tested using the universal primers for phytoplasma detection, P1/P7, in the first step.
The PCR products were re‐amplified with nested‐PCR to verify phytoplasma incidence using the
nested primers R16F2/R2.
Results: DAS-ELISA confirmed the presence of PNRSV in the tested peach trees. For phytoplasma
incidence, the univer

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

...tes originating from the same ancestral node however it is distantly related to other Egyptian strains 2005 and 2006 grouped together with a common ancestral node but on a separate branch. These results proved diversity between the isolated velogenic NDV Giza 2014 strain and other vaccinal and circulating NDV strains. Conclusion: It is concluded that further studies on the antigenic characters of that variant isolate is required to study antigenic variation be...

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and Mohammed A. Shalaby2

.../div>
releasing of safe, effective and potent product.
Objective: To use of some molecular techniques as conventional reverse transcriptase polymerase
chain reaction (RT-PCR) and real time RT-PCR in identity test of PPR vaccine.
Methods: Four batches of PPR vaccines that produced in Egypt by Veterinary Serum and Vaccines
Research Institute (VSVRI) were collected and tested for identification of PPRV by conv...
Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2
...v>Methods: The collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular fluids were
tested by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) for detection of
FMDV. The highly viral loaded FMDV positive samples were typed by conventional reverse
transcriptase polymerase chain reaction (RT-PCR). Sequencing and phylogenetic analysis of 6
rep...

Naglaa F.S. Awad1, Gamelat K.F. Kotb2

...: A total of 24 clinical samples were investigated by real-time reverse transcription PCR
(RRT-PCR). Hemagglutination analysis of RRT-PCR positive samples were performed, also these
positive RRT-PCR samples were purified and directly partial sequenced for C-terminal VP60 gene.
Results: Only two samples were positive fo...

Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden

... (ECE) or by molecular assay for detection AI virus specific nucleic acids such as (rRT-PCR).
Methods: Titration of the HPAI virus for infection of SPF chicken. The tracheal swabs were collected
from diseased chicken for virus isolation and quantification by ECE inoculation and rRT-PCR.
Results: the titer of original HPAI virus was 1010.3 EID50/ml. The AI titers after experimental
infection of chicken and viral r...
Rasha M. Mahrous1,4, Ahmed K. El-Attar2, Ahlam A. AlWatban1, Sohair I. EL-Afifi3,
Nagwa M. Aref1,3
...>detected in symptomatic samples using the specific primers of their 16S-23S rRNA gene by PCR.
Results: The Phytoplasma was isolated on indicator (Vollka marina) plants. It was transmitted on
Lemon (Citrus limon) by grafting and on periwinkle by dodder. It was detected in the sieve tubes and
parenchyma cells of leaf midribs tissues using Diene's stain and TEM. Infection of Lemon trees with
Phytoplasma associated ...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...nodular and crusted scab samples were collected from clinically
infected SPV sheep and were prepared and isolated on chorioallantoic membrane of 9-11 days old
emberyonted chicken egg for three passages. The isolated samples were identified by EM and PCR.
Results: Five samples were positive and showed a typical pock les...

Dina N. Abd-Elshafy

...traction. Cytotoxicity assay was
carried out according to Aquino (1989) to identify non-cyotoxic concentrations of the
prepared extract. Plaque reduction assay was performed to investigate the anti-HAV activity
of the green tea extract according to Tebas (1995), evaluate its effect, to test effect of
applying green tea extract against contaminated drinking water with HAV and st...

Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3

...iv>Background: Alfalfa mosaic virus (AMV) is only virus in the genus Alfamovirus and has very wide host
range among weed and crop plants which produces a variety of symptoms. It can cause problems in potato
in some regions where vectors easily move into potato fields from reservoir host, particularly if a tuber
necrosis-causing strain is involved.
Objective: : The purpose of this study is to characterize biologic...

Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2

...e virus (FMDV) is the causative agent of multiple outbreaks and
severe economic losses in the cattle population since its introduction in Egypt. Although vaccination is
practiced to control the disease, it is still endemic. Moreover, emergence of new strains is possible
through the international borders of the endemic countries. Consequently, there is a dire need for
routine molecular characterization of the circ...

Abdou Nagy1, Fatma Abdallah1, Kareem Sweed2, Ahmed Salama3, Mohamed Omar4

...iv>
Methods: Serum samples were collected from non-aMPV vaccinated apparently healthy 40 chicken
(23 broilers and 17 layers) and 8 duck (6 Pekin and 2 Muscovy) flocks and then, tested with
commercial indirect aMPV ELISA.
Results: Five out of 23 broiler, 6 out of 17 layer, 1 out of 6 Pekin duck and 1 out of 2 Muscovy duck
flocks showed p...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...e challenge trial. Blood samples
were collected weekly for 4 weeks of age and tested by HI test. Post challenge, tracheal and cloacal swabs
were collected at 3, 5, 7, and 10 days and tested by both real time RT-PCR (rRT-PCR) and virus titration
in SPF eggs.
Results: HI test revealed no significant difference between groups in the first 3 weeks post vaccination
and group 2 showed lower significant stat...

Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid

...
clinically affected samples (n=18) by RT-PCR using a specific primer set targeting a conserved
sequence within ARV- sigma C protein revealed 7/18 positive samples. All positive samples (7/18)
were successfully isolated on specific pathogen free embryonated chicken eggs (SPF-ECE). Additional
RT-PCR testing and re-isolation of ARV from ECEs of a breed...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
... experiment study. Blood samples were
collected for weekly for 4 weeks and antibody titers were determined by HI test. All vaccinated groups
were challenged 4 weeks post vaccination and tracheal and cloacal swabs were taken at 3, 5, 7, and 10 days
post challenge and tested by real time RT-PCR (rRT-PCR) and virus isolation and titration in SPF ECE.
Results: Results of HI demonstrated significant difference between...
Arslan Mahmood Ahmad1, Muhammad Ameen Jamal1, Abdul Sattar1,  Aamir Ghafoor2, Aqeel Javeed3 and Amjad Riaz1*
...ium oscillation inducing salt, is commonly used for the artificial activation mouse oocytes; however, limited reports are available to evaluate its negative impact. The objective of this study was to optimize the concentration of SrCl2 for artificial activation of mouse oocytes and to evaluate its toxic effect on early embryonic development.Our results indicated that higher percentage of activation of mouse oocyte was achieved with 10mM and 15mM for...
Ruqayya Bint Khalid1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Zubair Shabbir3 and Masroor Ellahi Babar4
...breed of Pakistan. Blood samples of Cholistani Cattle were collected from Government Livestock Farm, Jugait Peer, Bahawalpur. Genomic DNA was extracted from whole blood by organic method. PCR primers were designed and optimized according to respective melting temperatures. PRALINE tool, MEGA 6.0 and POPGENE tool were utilized for phylogenetic tree construction and statistical analysis, respectively. Characterization of physical and chemical properties of &beta...
Abdullah Sheikh1,*, Faisal Almathen1,2 and Hairul Islam Mohamed Ibrahim3,4
Zain-Ul-Aabdin Abro1*,Naheed Baloch1, Raza Muhammad Memon2 and Niaz Hussain Khuhro2
...uit fly Bactrocera dorsalis (Hendel) were monitored from January to December 2018 in guava orchards of the Sindh Pakistan. The investigations were done at different localities of Hyderabad and Larkana using Steiner type traps incorporated with methyl eugenol. The trap catches of B. zonata and B. dorsalis in both climatic zones shown a similar pattern during the study with a major peak in August in off se...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
...rent farms were randomly sampled and tested for the presence of A. marginale between May to August 2016. The study revealed that 15.0%, 20.8% and 29.1% of sampled animals were found positive for A. marginale using microscopic examination, indirect ELISA and PCR, respectively. Two major surface protein genes (MSP1a and ...
Wen-Feng Li, Rong-Yue Zhang, Jiong Yin, Hong-Li Shan, Xiao-Yan Cang, Zhi-Ming Luo, Xiao-Yan Wang, Jie Li and Ying-Kun Huang*
...lied for control of Sesamia inferens Walker and Argyroploce schistaceana (Snellen). The results showed that new sex pheromone trap of sugarcane borers in combination with Abamectin•Bacillus thuringiensis or Tebufenozide were the optimum mode for environmentally friendly control techniques of Sesamia inferens Walker and Argyroploce schistaceana (Snellen). The use of new sex pheromone tr...
Syed Maaz Nadeem1,*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh2, Arfan Ahmad2, Syed Nadeem Mansoor3 and Muhammad Anees4
...ler chicken. Bone marrow samples were collected from proximal tibiotarsus and femur of each of the five healthy and CAV-infected flocks under natural condition. The bone marrow smears were fixed, stained with Romanowsky Giemsa Stain (RGS) and examined under microscope. Results showed a significant difference between the cellularity of normal and CAV-infected birds. While comparing healthy and infected chicken bone marrow, we...
Abeera Razzaq1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3 and Qingyou Liu3
...i>by using micronuclei assay. Fish were exposed to the four sub-lethal doses (26.61, 13.30, 19.98 and 7.98 mgL-1) of Cr+Cr mixture for 28 days and blood was sampled after 7 days interval. A group (n=10) of fish were also kept in clean water (negative control=NC) and cyclophasphamid (positive control=PC), separately. Blood from caudal vein of fish was collected to see the micronuclei (MN) and de-shape nuclei (DN). ...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

...div>Methods: 130 Nodular samples were collected from 150 vaccinated cattle showing signs of LSD,
Then Molecular detection of LSDV with PCR using G-protein-coupled chemokine receptor (G-PCR)
gene primer revealed positive result for LSDV, So classical Isolation of LSDV was started by
inoculation of nodules on Chorioallantoic membrane (CAM) 9-11 day old SPF eggs, then adaptation
on Madin-Derby bovine kidney cell lin...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...s carried out. Sixty-two samples of whole blood with anticoagulant
were collected and screened for EHV-1 using nested PCR that amplify the conserved fragment of
glycoprotein B gene (ORF 33), followed by sequencing and phylogenetic analysis.
Results: The study revealed that 19% of our samples were positive to EHV-1 and phylogenetic
analysis showed that the Egyptian EHV-1 isolate...

Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1

...xamination. The positive samples were prepared and inoculated in SPF chicks' oneday-
old then at aged 10-days post-inoculation collected organs for PCR, sequencing and
immunohistochemically examination.
Results: There's one positive farm of age 100-days-old from Qalubia governorate. The
histopathological examination of positive sample showing severe depletion of hematopoietic c...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3
...to December 2017, 1500 nasal swabs were collected from
imported camels in Birqash veterinary quarantine, 195 nasal swab and Serum samples collected from
imported breeds in abattoirs of different governorates in Egypt, In addition to 98 nasal swabs and
serum samples were collected fro...

Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2

...y potent
and safe vaccines.
Aim: The present study investigated the effect of simultaneous administration of inactivated AI
vaccine and live attenuated TRT vaccine on the immune response of turkey chickens.
Methods: Four groups of bronzy turkey chickens were used in this study; group-1 received live
attenuated TRT vaccine, group-2 received inactivated AI vaccine, group-3 received TRT and AI

Ayman S. El-Habbaa1 and Mervat E. Radwan2

...
Israel during the same period suggesting that the virus was circulated in middle east.
Conclusion: These findings demonstrated the recent picture of BEF virus which incriminated for cattle
infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could
guide the application of efficient control strategies of BEF virus in Egypt.
...

Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1

...v>Background: Cucumber mosaic caused by Cucumber mosaic virus (CMV) is a serious threat to crop
production all over the world.
Objective: The aim of the current investigation was to evaluate the potential of fan-forced wind and
the growth promoting fungus, Fusarium equiseti GF 19-1, as novel and safe management strateg...
Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin
...>
Immuno-Sorbent Assay (DAS-ELISA). The PVM was mechanically isolated on potato cv. Diamant.
Host range was carried out by mechanical inoculation of PVM on a set of different plant species.
Electron Microscopy was used to examine the virus morphology. The cytopathic effect of the virus on
tissue ultrathin section was examined by transmission electr...
Samira M. Bolis1, Magda Mahmoud2, Salah E. Gumma3, Naser E. Bilal1 and
Isam ElKhidir4
...ytologically twenty nine samples 80.5% (29/36) which were positive for the PCR showed
koilocytosis and mitosoid cells. Some specimens were positive for more than one genotype. FEH was
more common in females than males. There was a significant difference between the PCR/Pap.
findings.
Conclusion: The most frequent genotypes which were detected were HPV 32 followed by HPV 16.
HPV genotypes 31, 33, 35, 5...

Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2

...us isolates of Alfalfa Mosaic Virus (AMV) found throughout the world
affect a wide variety of aromatic and medical plants. In March 2016, several symptoms of leaf
necrosis, bright yellow mosaic and malformation of leaves suggested viral infection of AMV on
basil plants grown in Beni Suef governorate.
Objectives: The present study aimed to characterize the virus at the molecular...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
...ular characterize IBV in samples
collected from Lebanese flocks geographically distributed in 7 Governorates.
Methods: Blood sera, tracheal swabs and tissue organs were collected from 60 poultry flocks from
different ages, different type of raising which were housed in different Governorates in Lebanon
including, broilers, layer hens of breeders, backyard chickens and ducks for serosurveillance and
mo...

Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi

...
Results:Testing of samples with rt-RT-PCR revealed that 6 flocks reared in 4 Lebanese governorates
were positive (CT ranged from 20.42 to 33.86). A Positive sample with CT 20.42 was tested and
partially sequenced and submitted to GenBank, acquired accessions number MH266696 and
designated A/chicken/Lebanon/21-E03/2018 (H9N2). Phylogenetic analysis of the obtained sequence

Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2

...ackground: Infectious bursal disease virus (IBDV) remerged frequently in Egypt, causing serious
economic losses. In Egypt very virulent (vv) IBDV strains are annually reported since its first
introduction in 1989. Variant IBDV strains were also reported in few studies. To date, IBDV
associated with breaks has been characterized in flocks which were vaccinated with classical IBDV
vaccines and demonstrated IBD char...

Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2

... study, we collected 122 sample representing 12 different area in Egypt and seven different species of poultry produced and traded in Egypt. These samples were isolated on SPF eggs, detected for AIV infection Via HA, RT-PCR and gene sequencing. We analyzed the sequence on gene bank to compare it with the other AIV strains
Results: We found 35 and 55 samples showed positive resu...

Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2

...tracted from symptomatic samples was
used as a template in nested polymerase chain reaction (nested-PCR) using universal primer pairs
P1/P7 and R16F2n/R16R2. Sequencing and phylogenetic analysis were performed to identify the
detected phytoplasmas.
Results: Phytoplasma was transmitted successfully from naturally infected gazania to healthy
ornamental plants by dodde...
Hanan Sayed1, Mohamed S. Madkour1, Hussein A. Hussein2, Mounir Mohamed Elsaft 3,
Marwa F. El saied3, Ahmed A El-Sanousi2
...n.
Results: Usage of formalin inactivated combined H9N2, LaSota and IB (M41 and Var 2) vaccine with
montanide 71 adjuvant decreases the shedding pattern of H9N2 and NDV in SPF and commercial layer
chicken for six months after the booster vaccination.
Conclusion: Formalin inactivated, montanide 71 adjuvanted combined H9N2, LaSota and IB (M41
and Var 2) vaccine induces protection for 7 months against ch...

Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2

.../div>
Conclusion: Usage of formalin inactivated combined H9N2, LaSota and IB (M41 and Var 2) vaccine
with montanide 71 adjuvant can protect chicken until six months from the booster vaccination and
decrease shedding of IBV.
...

Ahmed M. Soliman

...v>Background: Cucumber mosaic virus (CMV) is one of the widest spread viruses in the world and has the largest host range of plants.
Aim: The present study was conducted to identify four Saudi CMV isolates (cucumber, tomato, pepper and watermelon) using biological, serological and molecular assays.
Methods: Survey of some vegetable crops exhibiting mosaic, yellowing...

Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2

...ackground: Bean yellow mosaic virus (BYMV) is recognized as one of the most wide distributed virus disease affecting faba bean in Egypt.
Objective: The aim of this study is to explore the efficiency of the plant growth promoting fungi (PGPF) of Penicillium simplicissmum GP17-2, Fusarium equiseti GF19-1 and Trichoderma asperellum SKT1 as a sustainable method to control the invasion of BYMV in faba bean plants.

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

...immunize the cattle with safe vaccine .Ismailia isolate of LSD was propagated and titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 5.5 TCID50/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characterization of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37°c. The inactivated LSDV tested on MDBK cells and the animals fr...

Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein

...phocytic proliferation assays demonstrated the continuous response of lymphocytes started at the 3rd day till reaches peak at 28th day post vaccination then start to decline. Whereas the peak titers for antibodies were reported at the 28th day post vaccination. Indeed, the study reports the role of ail adjuvant in stimulating the cellular response for inactivated vaccine.

...
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...iv>using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Host range study
was carried out using mechanical inoculation into fourteen different diagnostic host plant species and
cultivars belonging to 6 families. Tomato seeds were treated with four different concentration of
NaN3and EMS (1.0, 2.0...

Tahsin S. Shoala1, Ahmed K. El-Attar2, Ahmed I. Abd El Maksoud3

...cid ammonium
salt (GAS), Glycyrrhizic Acid (GA), Curcumin (CU) and Rosmarinic Acid (RA) were
sterilized and added to MS media at different concentrations.
Results: Potato plants those treated with Glycyrrhizic acid ammonium salt (2ng, 4ng, and 8
ng/ml MS media) showed that the presence of PLRV at concentration 2ng/ ml MS media
while they were negatively diagnosed fo...

Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon

...ivity using corn bran (dusa) as alternative vaccine carrier in five
Local Government Areas (LGA) in Adamawa state, Nigeria.
Method: Structured questionnaire on general knowledge, practice and productivity indicators was
administered thrice to participating household poultry farmers alongside administration of feed based
V4 thermostable ND vaccination in chickens. Sera obtained from forty (40) chickens per LGA dur...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...>
(SPPV) giving un-satisfiable results and incomplete protection in vaccinated cattle, since that it was a
must to produce a homologues vaccine for LSDV.
Methods: A local isolate of LSDV strain was propagated on MDBK cell line and CPE was appeared
after 72 hours as cell rounding, cell aggregation and cell degeneration with titer of 105.5TCID50/ml,
DNA extraction and PCR for the candidate strain was performe...
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
...s used to detect LSDV in sampled ticks. PCR-positive tick samples were subject to a
modified RT-PCR assay to detect the polyadenylated LSDV DNA polymerase mRNA. Metagenomics
analysis using MinIon technology was used to generate LSDV sequences and to investigate relative
abundance of LSDV sequences in the sampled tick p...

Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2

...s used for six serial passages.
The obtained LSDV isolate was molecularly identified and deposited to the GenBank accession
number of MN271728. In addition, a comparative study of LSDV growth curves of two permissible
continuous cell lines (MDBK and Vero). The growth curve of LSDV was determined by inoculating
both cultures, harvesting at intervals up to 24 hours and titrating the virus culture. To confirm LSDV
Soad, E. Elsayed 1, Mohammed A. Shalaby2, Ausama A. Yousef2, Abu bakr A. Agor1,
Sherouk E. Aly1
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
...
trehalose as dispersant and measuring different physical characters including particle size, Zeta
potential, loading capacity and imaging of adjuvant before and after formulation.
Results: Ultrasonication of aluminum hydroxide gel resulted in generation of 120-200 nm gel
structures followed by vaccine formulation showed enhancement of vaccine loading capacity. Animals
vaccinated using the enhanced formula w...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
...
inhibition (HI) assay, protection post challenge, virus shedding and transmission in contact
non vaccinated chickens using qRT-PCR test.The genotype VII (GVII) vaccine group
induced higher HI titer by using GVII monospecific antigen and decreased virus shedding
than other group. The vaccinated group with genotype VII significantly reduced the virus
shedding at 3, 5, 7 and 10 days post challenge unlike the ...

Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder

...v>Background: Cucumber mosaic virus (CMV) is known to occur in sugar beet plants in Egypt and may
produce severe damage to infected plants. However, studies on the effect of the CMV on the cellular
and internal structures of sugar beet leaves were rare.
Methods: The CMV was isolated from sugar beet samples collected during November 2018 from the
Fayoum governorate, exhibited sy...

Muhammad Ehsan Safdar1*, Samra Ammara1, Muhammad Sikander Hayyat1, Shahbaz Hussain2, Muhammad Mansoor Javaid1, Abdul Rehman1 and Amjed Ali1

Md. Abdullah Al Mamun1,2*, Shamima Nasren1,3, Sanjay Singh Rathore1 and Kavalagiriyanahalli Srinivasiah Ramesh1

...ermanganate II) Aquarium salt III) Formalin and IV) Mechanical. The significant lower (P<0.05) parasitic burden were found in mechanical treatment followed by salt, formalin and potassium permanganate. Clinical signs like excessive mucus production, fin erosion, pale gills, and slight hemorrhage were observed in the affected area of skin and operculum. Among the internal organs, liver lost its original reddish-brown and t...

Gulnaz Ismaylova

...ards was carried out. Orosanga japonica first recorded on Morus sp. in the Sheki-Zagatala region located in the northwest, and then in the Khachmaz region, located in the northeast of Azerbaijan. Representatives of this family have not yet been registered in Azerbaijan. Besides of mulberry, the pest was registered in 11 more plant species. The article provides information on the distribution, phenology and plants affected by the pest. Both nymphs and adults of...

Aliya Munir1, Sajid Abdullah1*, Huma Naz2*, Khalid Abbas1, Tanveer Ahmed3 and Zahid Farooq2

... 24-hr period. Fish were sacrificed and organs were collected to calculate the POx activity. It was observed that the exposure of DM+ES significantly accelerated the POx level in gills, liver and kidney of fish in a time dependent manner.  

...

Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski

... medicine. In this study samples were taken from three points in the broiler meat production (farm, slaughter line and cold storage of the meat before shipping to the market). A total of 283 samples (cloacal swabs, caeca and carcass swabs) were analyzed for the presence of C. jejuni. The isolates of C. jejuni were confirmed with the conventional microbiological method and with the use of multiplex PCR metho...

Anila Kousar, Muhammad Naeem*, Samrah Masud, Abir Ishtiaq, Zara Naeem and Rabia Iqbal

...d for T1, T2 and T3 fish samples. An increasing trend was observed in the concentrations of studied elements with the increase of dietary crude protein in fish feed. The elemental concentration in the carcasses of studied GIFT yielded similar accumulation pattern for three treatment groups T1, T2 and T3, i.e., K > Na > Ca > Mg > Fe > Zn > Pb > Cd. Linear and multiple regression was applied to check the significance of accumulated elements....

Nadeem Anwar1, Muhammad Luqman2*, Shaoib Nasir3, Moazzam Sabir1, Hafiz Bashir Ahmad4 and Saleem Ashraf5

...rmers were found to be unsatisfied about the role of intermediaries and termed these intermediaries as profit-making group indenting the share of farmers. The Harvesting behavior of the farmers is significantly affected by the area under citrus cultivation. The results also indicated that product marketing and harvesting behavior of citrus growers is positively associated with a unit increase in area under citrus cultivation. The analysis of Socio-economic cha...

Bakhtiar Gul*, Saleetha Rabial and Haroon Khan

... were recorded for glyphosate as compared to weedy check (4.45 g pot-1, 58.7 % and 22.67 %, respectively). Moreover, highest plant mortality (24.33 %) after four weeks was noted in glyphosate. Glyphosate effectively controlled the duckweed, followed by MCPA as compared to bromaxonil, isoproturon and paraquat. Therefore, glyphosate and MCPA proved suitabl...

Ayesha Manzoor1, Muhammad Saqib Naveed1, Sayed Rashad Ali1, Danish Ibrar2, Sairah Syed1, Sharmin Ashraf1 and Rafiq Ahmed1*

Ibadullah Jan1*, Iqbal Munir2, Inamullah Khan3, Syed Muhammad Suhail4 and Aqib Iqbal2

...ained from Dodonaea viscosa, as an anti-inflammatory, antipyretic, and analgesic along with in vivo acute toxicity in the animal model. Different techniques such as xylene induced ear edema model, Carrageenan induced paw edema model, Hot plate pain model, Ethanoic acid-induced pain model, Yeast instigated pyrexia model acute toxicity model was used to investigate Dosiflavone. Dosiflavone showed significant (p<0.05) reduction of the ear edema at a high dose....

Ameer Hamza1, Mukkram Ali Tahir1, Noor Us-Sabah1, Ghulam Sarwar1 and Muhammad Luqman2,*

...e brackish water. Use of saline water along with leaching fraction could be useful by keeping low concentration of salts in the root surrounding area. This study assessed the role of leaching in alleviating the negative effect of saline water on soil characteristics. Results of the studies indicated that 6.57% reduction in soil pH (7.6), 46.06% reduction in EC (1.3 dS m-1), and 46.41% redu...

Shakeel Ahmad1, Muhammad Israr2*, Muhammad Amin3, Muhammad Sadiq Hashmi4, Nafees Ahmad5 and Rasheed Ahmad6

...asses: evergreen forest, savannas, grasslands, permanent wetlands, croplands, natural vegetation, and barren land. The result indicated that grasslands (17.04–12.84%) and cropland (34.73–18.12%) decreased significantly due to over population pressure coupled by natural hazards, while savannas (40.63–49.25%), permanent wetlands (0.03–0.07%), and natural vegetation (3.13–14.96%), were increased si...

Md. Sahidur Rahman1*, Tishita Ape1, Md. Islam1 and Sharmin Chowdhury2

...s a major hazard to food safety and leads to the development of antimicrobial-resistant microorganisms. We conducted a cross-sectional study to assess the perspective of dairy farmers regarding antibiotic use as well as its residual and resistance effect. We collected data from 101 dairy farmers in Chattogram, Bangladesh. The results revealed that ceftriaxone, gentamicin, streptopenicillin, amoxicillin, and sulfur drugs were the most frequently used antibiotic...
Jehan Dastagir, Muhammad Amir*, Bilal Ur Rehman, Shahid Hameed and Majad Ashraf

Iftikhar ud Din* and Yousaf Hayat

... Data on two parameters (say plant height and yield) are simulated with varying magnitude (low, moderate, and high) of correlation coefficient between the two response variables. The comparative analysis of MANOVA and ANOVA reveals that even a small amount of correlation between the dependent variables creates huge change on the status of main effect and interaction of the three independent variables. The results reveal that when linear relation between traits...

Ghani Akbar*, Muhammad Asif and Zafar Islam

....) and cucumber (Cucumis sativus) under tunnel farming compared with traditional open-air farming method in Potohar, a rainfed area in Pakistan. The results indicated increased production potential per unit of land (46%) and per unit of water (74%) and larger farm income (3.47 BCR) than the traditional open-air farming method. These production benefits can be instrumental in improving livelihood and nutrition of remotely located rain-fed farmers in the country...

Nusrat Habib

...den doubled during the disaster days comparing with their male member’s counterparts. Women contribute significantly to adaptation and resilience to climatic extremes. The drivers of their resilience are hard work and diversity of livelihood resources. In changing climate conditions, it is imperative to devise gender-sensitive and region-wise policies and planning.

...
Song Jiang1,2,3, Xianbin Mo1,2, Falin Zhou2,3, Jianhua Huang2,3, Qibin Yang2,3, Lishi Yang2,3 and Shigui Jiang2,3*
...erefore, it was not necessary to establish different breeding lines for different feed conditions in the range of 10% - 30% protein level of feed fish meal.
...

 Yongmin Li1,2, Huabin Zhang1, Xiaoyou Wu1, Dongwei Li 2, Peng Yan1 and Xiaobing Wu1*

...geri) occupied the basal position in the clade of Rhacophoridae.
...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
...tion and wound healing assays, while the auto-fluorescent property of DsRed was confirmed by fluorescence microscopy and immunofluorescence. The potential application of his-AGR2-DsRed was demonstrated by its internalization in fibroblasts. Therefore, the purified recombinant fluorescent protein would be a very useful tool for further investigation of AGR2’s extracellular function and molecular mechanism.
...
Jing Yu1*, Yao Lu1,2, Zhaojin Lin1, Pimao Chen1 and Yuting Feng1,3
...linear regression, using satellite remote sensing and investigation data during April to June in 2014 and 2015. Satellite data including Sea Surface Temperature (SST), Sea Surface Salinity (SSS) and water Depth from Digital Elevation Model (DEM) were used to build the Habitat Suitability Index (HSI) model, based on Maximum Model (MAXM), Minimum Model (MINM), Arithmetic Mean Model (AMM), and Geometric Mean Model (GMM). Results showed that the accuracy of the HS...

Syed Ismat Hussain1, Khalid Mehmood2, Mudassar Khaliq3, Habib Anwar1, Syed Muhammad Zaka4, Ateeq ur Rehman5*, Muhammad Shahid6, Syed Atif Hasan Naqvi5*, Ummad ud Din Umar5 and Muhammad Asif Zulfiqar7

Rupinder Kaur1,2*, Pretty Bhalla3, Jubin Kumar Saini4 and Sayeeduzzafar Qazi5

...loring its effect on job satisfaction, job performance and well-being of employees, and testing and implementing coping measures is suggested for future studies. 

...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
... testis. Blood and semen samples were collected from clinically diagnosed 130 oligozoospermic males throughout D. I. Khan. The patients included had been married for 3 to 19 years, their wives had normal reproductive capabilities; the couples were living together for more than 3 years but fertilization had not occurred. Separation of sperm samples from semen plasma, sperm counting and DNA extraction for assessment of clinica...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1,  Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...ated with MDV, and blood samples of two groups were obtained at 14 and 22 days post-infection (dpi) for RNA extraction. Transcriptome analysis was performed to explore the immune responses of chicken erythrocytes to MDV infection at 14 dpi and 22 dpi. In total, 129.6 million reads of two groups at two time points were mapped against the reference genome (Gallusgallus 5.0). A significant differential pattern of the immune response of chicken erythrocytes was id...

Dilan Ozmen Ozgun1, Gonca Alak2, Arzu Ucar2, Sinan Bilginer3, Halise Inci Gul3, Mahmut Kocaman2, Cem Yamalı3, Veysel Parlak2, Muhammed Atamanalp2, Ahmad Maslat4 and Telat Yanık2*

...DMSO for 96 hours. Blood samples were collected for hematological analyzes and mortalities were recorded daily. Exposure to B4 caused significant changes in antioxidant enzymes of gill and liver of alevins (p<0,05). Catalase, superoxide dismutase (SOD) and glutathione peroxidase activities were decreased, while MDA values increased significantly (p<0.05) in gill and liver of fish compared to control group. SOD activity was time dependent; it decreased in...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...spp.beef meat samples were collected from the local butcher shops (n=75) of Karachi, Pakistan. Three types of meat samples were collected viz., muscles, lymph nodes and minced meat (n=25 each). The samples were packaged aseptically and transported to laboratory within 4-6 hs of collection in cooling conditions. The samples were examined for
Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*
...he tick Rhipicephalus sanguineus (Latrielle) that causes canine monocytic ehrlichiosis (CME) in dogs. We have investigated the prevalence of R. sanguineus and E. canis infection in pet dogs in Wenzhou, China. Serum samples were obtained from animal hospitals were examined for antibodies by commercial rapid in-clinic ELI...
Wenjuan Yan1, Qunlan Zhou2, Bo Liu2,3*, Cunxin Sun2, Huimin Zhang3 and Changyou Song2
...igate the effects of Isaria cicadae Miquel (I. cicadae) on growth performance and meat quality of giant freshwater prawn (Macrobrachium rosenbergii). The prawns were fed with five isonitrogenous (40.54% crude protein) and isolipid (6.16% crude lipid) diets with different I. cicadae levels (0, 100, 200, 400, and 800 mg Kg-1). Results revealed that I. cicadae significantly increased specific growth ratio (SGR) and pr...
Muhammad Khaled Siddiq1, Muhammad Adeeb Babar1,2*, Muhammad Akbar Khan1, Muhammad Umar Ijaz3,Sayyed Ghyour Abbas1, Muhammad Asim1Asif Mahmood Qureshi4, Mahboob Iqbal4 and Muhammad Akhtar4

Zain Ullah1*, Anwar Ali Shad1 and Saqib Ali2

...ues. The antibacterial assays of the obtained six oil sub-fractions (H-1 to H-6) were accomplished against S. epidermis, E. coli, and S. aureus bacterial strains and the results ranged from 10-18 mm zone of inhibition. The GC-MS analysis of the six oil sub-fractions (H-1 to H-6) confirmed the existence of linoleic acid, palmitic acid, and oleic acid in the highest amount.

...

Sana Javeid* and Khalid Nawab

...multistage simple random sampling techniques. The data were collected using an interview schedule; analysis were carried out by using cross-tabulation, frequency counts, percentages through Statistical Package for Social Sciences (SPSS). To measure the poverty level of apple orchard growers, poverty score card was employed. In addition, bivariate analysis was used to examine relationship between various extension practices and poverty. Results of the study rev...
Nabila Kousar1, Tanzeela Riaz2, Anum Feroz1, Abdul Rauf Shakoori3 and Farah Rauf Shakoori1*
... also tested against the same larval stage of different strains of pest populations and the mixture 0.5DM+1DE was found to be most effective among all mixtures on the basis of LC50 values and relative toxic unit. The toxicity of this mixture was analyzed on glucose, glycogen, trehalose, total proteins, soluble proteins, free amino acids and total lipids contents of 4th instars larva of most resistant GUW and least resistant LAB-S populati...
Atta Muhammad Memon1, Ubedullah Kaka2*, Muhammad Umer3, Asghar Ali Kamboh4, Atique Ahmed Behan5, Habibullah Janyaro6, Yong Meng Goh7, Amir Bukhsh Kalhoro6Jalila Abu8, Khaleeq ur Rehman Bhutto9 and Arsalan Maqbool10


...ch are respiratory depressants to ensure safe or balanced anaesthesia. The timing of administration and the effect on recovery for specific alhpa2 agonist antidotes in birds have not been widely investigated. This study was conducted to assess the reversal effects of atipamezole administered at the first sign of recovery on medetomidine-ketamine anaesthesia in pigeons. Twelve pigeons were ...
Muhammad Mobashar1, Qazi Aqeel1, Muhammad Tahir Khan1, Assar Ali Shah2*, Ahmed A.A. Abdel-Wareth3, Salman Khan4, Nazir Ahmad1, Sami Ullah1 and  Haq Amanullah5
...in-vivo and in-saco nutrient degradability in Kari sheep. A total of 36 Kari sheep were randomly weighed and offered the following four type of ration: A, total mix ration (TMR) + alfalfa (AL) 70 % and Prangos pabularia (PP) 0 % (control); B, TMR + 24% PP: 46 % AL; C, TMR + 46% PP 24 % AL and D, TMR + 70% PP: AL 0 % on dry matter basis were fed to experimental sheep for a period of 90 days. The dry matter intake, crude protein intake and o...

Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1

...tured plants contain the same secondary metabolites as the wild type. This study aims to identify the chemical content of two varieties of O. aristatus resulting from in vitro culture. The five-month-old variety of O. aristatus purple and white purple and wild type was extracted using two solvents with different polarity levels, namely ethanol and ethyl acetate. The concentrated extract was identified for its chemical content using gradient system HPLC 0.1% fo...

Muhammad Madni Afzal1*, Shahbaz Talib Sahi1, Amer Habib1, Waqas Ashraf2, Muhammad Ahmad Zeshan3, Muhammad Raheel2 and Qaiser Shakeel2

...its yield and quality. Fusarium oxysporum is a very common soil-borne fungus that causes basal rot onion and it has the ability to survive in the soil for many years without having a host. Many fungicides are available in the market to control the basal rot of onion which contain hazardous material that continuously destroys the environment and human health. The objective of the present st...
Syed Azmat Ali Shah1*, Asfandyar Ahmed2, Khan Shahzada3, Syed Muhammad Ali4, Akhtar Naeem Khan5 and Akhter Gul6
...emonstrated that for the same gravity load level the enhancement of brick unit properties enhances the total capacity of the RC frame with infill. Similarly, by increasing the gravity load on the structure with the same brick unit properties, the lateral load capacity increases considerably.
...

Miloudi Hilali1*, Hanae El Monfalouti1, Larbi El Hammari2, Farid Amar3, Zakaria Mennane4, Malika Haddam5, Nadia Maata6 and Badr Eddine Kartah1

...ges of maturity from the same tree in October, November and December 2020, the extraction was made by mechanical cold pressing. After extracting the olive oil, the physico-chemical analysis such as acidity, humidity, fatty acids and sterols was assayed. The results obtained the sample which extracted in the month of december contains a higher percentage of β-sitosterol (95.2%) and hig...

Ghulam Muhiyuddin Kaloi1* and Mehrunisa Memon

...xygen demand and soluble salts. Its injudicious use may be hazardous for soil health. Study was conducted to evaluate significance of wastewater in context of sugarcane juice quality and soil health. Wastewater was tested on experimental field of Matiari Sugar Mills in a randomized complete block design with factorial combination of five concentrations of wastewater (0, 5, 10, 15 and 20%) and four rates of inorganic fertilizers (0, 1/3rd, 2/3rd and full) besid...

Javed Anwar Shah1, Azhar Iqbal1, Muhammad Tariq Mahmood2, Muhammad Aslam3, Muneer Abbas4 and Ilyas Ahmad5*

... is considered as most disastrous and widespread fungal disease of chickpea across the world. Under favorable environmental conditions AB epidemics may cause partial to complete yield loss. Chickpea faces several biotic and abiotic stresses. Among biotic factors chickpea blight is most important factor responsible for drastic decline in productivity. Other disease management approaches are not more efficient and economical except to exploit the host plant resi...
Asim Mushtaq1*, Asra Nafees1, Raza Muhammad Khan1, Amina Israr1 and Zaeem Uddin Ali
...in sight of fly residue, saving might be up to 35 percent. All these benefits result in the overall enhanced durability of concrete. 
...

M. Azam, Iqtidar Hussaian*, Ghazanfar ullah, A.A. Khakwani, M.S. Baloch, K. Waseem, M. Amjed Nadeem and M.K. Javaid 

Muzamil Farooque Jamali1, Fayaz Ali Jamali1, Tanveer Fatima Miano1, Zulfiqar Ali Abbasi2, Sohail Ahmed Otho3, Khalid Hussain Talpur4, Niaz Ahmed Wahocho1 and Muhammad Iqbal Jakhro5*

...e mix) were subjected to salinity stress condition (0, 2, 4, 6 and 8 dS m-1). The results exhibited that salt stress revealed adverse effect on all the parameters of both of the varieties of marigold. The plants irrigated with canal water (control) having EC of 0.7 dSm-1 showed better results for both seed and flowering related traits. The plants treated with canal water showed better seed germination (82.56 %), seed germina...

Izaz Hussain, Ahmad Khan* and Habib Akbar 

...justify;">The useful and safe disposal of farmyard manure (FYM) is required for avoiding the environmental pollution. However, the bulky application of FYM and slow decomposition limits its widespread application by farmers as a source of nutrients. With the objectives of improving soil mineral N availability and its impact on maize growth, two years field experiments were conducted at the University of Agriculture Peshawar ...

Hafsa Naheed* and Hidayat-Ur-Rahman

...he genotypes was not the same across the environments. Various stability parameters; Eberhart and Russell, Perkin and Jinks and Finlay and Wilkinsons models were used to determine stability. Considering the Eberhart and Russell’s approach G.27, G.34, G.11, G.17, Siran and G.35 for tillers m-2 and G.22, G.23, G.31 for kernel weight had the bi (regression coefficient) values near to unity and had higher (coefficient of determination) Ri2. Based on the stab...

Sajid Abdullah1*, Huma Naz2*, Zunera Abbas1, Uzma Nazir1, Mehrunisa Basharat1, Tanveer Ahmed3, Adnan Ahmad Qazi2

...sessed by micronucleus assay. Fish were exposed to different concentrations (1/3rd, 1/4th, 1/5thand 1/7th of LC50) of Pb, Cr and Cd, separately, for three weeks. Blood sample of fish was collected to see the micronuclei (MN) and deshape nuclei (DN) in peripheral erythrocytes of fish after one-week interval. Fish W. attu showed significant induction of MN and DN in blood due to metals (Pb, Cr and Cd) exposure. It was noted th...

Sajida Batool1, Saira Batool1, Sitara Shameem1, Fatima Khalid1, Tahira Batool2, Summera Yasmeen1, Saima Batool3*

...th day, all animals were sacrificed to recover liver for histological preparations. Atrazine (ATZ) caused various hepatic implications in the treated animals. ATZ exposure caused a significant decline in mean body and liver weight as compared to the Cnt group. The histopathological findings in ATZ administered group included loosening hepatic tissue, massive degeneration of hepatocytes, deformed nuclei of hepatocytes, highly disordered trabecular arrangement, ...
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...rcus) stearoyl-CoA desaturase 1 (SCD1) plays a crucial role in fatty acid metabolism including milk and muscular fatty acid. This study investigated the expression of SCD1 gene in goat and examined its inheritability and expression in transgenic SCD1 mice and goats. Our results suggested the possibility of generating transgenic goats by sperm-mediated gene transfer (SMGT). The expression of SCD1 gene and fatty acid metabolism genes in goat ma...
Tanmoy Mandal1 and Sunil Kr. Ghosh2*
...ilk worm (Antheraea assama West wood) is reared on leaves of som plant (Machilus bombycina King) because of their high nutritional value. Rearing becomes difficult due to attack of large number of insect-pests on som plant. Leaf miner(Phytomyza spp.)(Diptera: Agromyzidae) is a major pest and very harmful to som plant leaves. From observation it is found that leaf miner was found active throughout the year on som plant leaves. Higher...
Ahmet Arpacık
...e="font-size: small;">Dorsal guard hairs from three canid species that included red fox (Vulpes vulpes), golden jackal (Canis aureus), and gray wolf (Canis lupus) found in Turkey were characterized using light microscopy to study hair features, including cuticle and medullary patterns, medullary index, and hair length and root. The morphological features of the medulla and cuticle structures were quite similar for the three species, but a ...
Chao-Qun Shi1, Hong-Ying Lin1, Jin-Qing Zheng1, Fan Yang1, Kwame Ayisi Lartey1, Dan-Ju Kang1, Hwa-Chian Robert Wang2, Ravi Gooneratne3*and Jin-Jun Chen1*
...The groups were control (saline), negative control (E. coli + 0 mg/kg TFCO), low dose (E. coli + 10 mg/kg TFCO), medium dose (E. coli + 40 mg/kg TFCO), and high dose (E. coli + 160 mg/kg). TFCO was administered by gavage 3 h post intraperitoneal (i.p) E. coli injection. Serum IgM, IgA, IgG, TGF-β1 mRNA expression in duodenal villi, and histopathology of duodenum were also studied. TFCO significantly increased the ...
Zhengyi Fu1,2,3,4, Rui Yang2,3,4, Zhenhua Ma2,3,4*, Mingyang Han2,3,4 and Yifu Wang2,3,4
...nd the height of the mucosa in the HL were significantly higher than those in the other two groups. The lipase activity of HL and HC was higher than that of control group in both stomach and foregut. This study indicates that increasing the proportion of lipid in the dietary improves growth performance and digestion performance of barramundi, while higher proportions of carbohydrate in the diet don’t affect their growth performance. Considering the cost ...
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...otal of 334 chicken meat samples, 197 eggshells and 160 human stool specimens were included in this study. The presence of salmonellae spp. was examined using bacteriological isolation and serological identification. PCR amplification of the ESBL and carbapenemase-encoding genes was performed. The bacteriological examination of the samples showed that 6 Salmonella strains [S. Typhim...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1
Xiu-Juan Zhang1 and Jin-Ping Chen1*
... tested in 39 individual samples. For parentage testing, the allele frequency date-based indicated that the combined exclusion probability values were over 99.99% when 9 SSRs were used. This study demonstrated that the microsatellite-based approach could be effectively utilized for parentage analysis in pangolins and that it has a significant application in selective breeding in endangered and valuable mammalian species.
Masoomeh Sohrabi Mollayousefi1* and Babak Moghaddasi2
...oughout the country. The sampling stations were selected to cover the shoreline both extremes as well as middle parts. Different physio-chemical properties like salinity, pH, carbonate content, sediment particle size and peculiarities were measured in the pre-defined sampling sites. Our findings show that Foraminifera and Ostracods abundance negatively dependent to the sediment particles&r...
Kang Chen1,2, Dekui He1, Chengzhi Ding3,4, Yintao Jia1 and Yifeng Chen1*
...e was produced by Bochu Tsangpo, the differences in otolith shape among 11 stocks were not very noteworthy. The information generated in this research can be used as a reference for stock discrimination of G. selincuoensis from the Tibetan Plateau. Further research is needed to explore the causes of shape variation.
...
Wanqiu Zhao1,2, Taoyan Yuan1, Li Chen2, Xue Du2, Weihu Chen4, Yan Fu1, Dong Niu3,* and Lizhi Lu2,*
...f geese duodenum content samples at three different stages (laying, broody, and recovery stage). Taxonomic analysis revealed that Firmicutes, Actinobacteria, and Proteobacteria were the three most abundant phyla in the duodenum of the laying geese. Cyanobacteria predominated in broody geese, followed by Firmicutes, Proteobacteria, and Spirochaetes. The microbial communities of the recovery geese were dominated by Spirochaetes, Firmicutes, Cyanobacteria, and Pr...
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
...gastric gland and submucosa of the stomach, the submucosa, muscular layer and intestine villi of the intestine, the granulose cell and follicular membrane cell of the ovary and the interstitial cell and seminiferous tubule of the testis. Moreover, we investigated seasonal changes of leptin in plasma of Chinese alligator by radioimmunoassay method. Our results demonstrated circulating lepti...
Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
...rmation. The flagellar basal body has an ancient and evolutionarily conserved macromolecular assembly and known architecture making it an ideal drug target. The knowledge obtained will help to elucidate mechanism and design principles necessary to understand protein secretion systems.
...

Kawa A. Ali1*, Sazar S. Noraldeen2 and Arshad A. Yaseen2, 3

...>) instruments, then the same leaves were cut and chlorophyll estimation were done by destructive methods using two organic solvents ( 80% acetone and 100% ethanol). Results demonstrate significant differences between SPAD and atLEAF data for tomato and pepper plants where higher levels with atLEAF instrument comparing to SPAD chlorophyll meter. Higher chlorophyll levels were recorded in open field plants comparing to lath and plastic houses plants. Chlorophyl...

Muhammad Waheed* and Dost Muhammad

...abile P at all the three sampling times. However, the moderately labile and non-labile fractions showed non-significant changes with time. The Plant height, biomass and total P uptake were found significantly higher in the treatment fertilized with 2 % TSP enriched compost followed by 4 % TSP enriched compost as compared to control. The effect of TSP enrichment was better than RP as well as the enrichment with 2 % was better than 4 % for both sources in both s...

Khaliq Dad1, Muhammad Nawaz2*, Muhamamd Ibrahim3, Fengliang Zhao4, Rumsha Hassan2, Humaira Nawaz5, Muhammad Usman Saleem6, Kinat Javed2, Ayesha Komal2 and Hajra Naz2

...oremediation phytostabilisation, rhizofilteration, phytoextraction. This review paper has summarized the impact of Cd on soil, plants and humans and strategies to remove or minimize its toxicity by applying some low cost and environmental friendly techniques. 

...
Yanhong Hu* and Linkai Cui
... an immunofluorescence assay of the insect cells and a bioinformatics analysis. The result of immunofluorescence assay showed EpFAR to be localized to the cytoplasmic region, and the observed fluorescence pattern was characteristic of endoplasmic reticulum (ER) proteins. Comparative analysis of the prediction results showed EpFAR was localized to the ER membrane. These results, combined with the localization of other FARs, s...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...s isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shanghai, Sichuan, and Gansu. The results showed that the amplified product bands with three primers per isolate ranged from 1~9 and the produ...
Li Ma1 *, Xu Han1, Bo Yang2 and Chunhua Zhou3
...yltetrazolium Bromide) assay was applied in hydrogen peroxide-treated human umbilical vein endothelial cells (HUVECs). The hydrogen peroxide-induced apoptosis was gauged using flow cytometry whereas western blotting was applied to detect the expression of key cellular markers in oxidation pathways. The data indicate that a total of 50 µmol/l concentration of kaempferol can profoundly protect the oxidative damage in HUVECs. However, compared to the oxidat...
Junyan Bai1*, Rentaodi Wu2*, Qihang Hu1, Lema Dao2 and Xinghua Tian3
...correlation between microsatellite marker polymorphism and wool fineness of sunite bactrian camel was analyzed. The results show that, the average effective allele number, heterozygosity and polymorphism information content of 17 microsatellite markers in sunite bactrian camel were 2.8913, 0.6205 and 0.5602, respectively. The wool fineness of AB genotype of CMS36 locus was significantly higher than that of BB genotype (P<...
Arsalan Khan1*, Navid Anjum2, Muhammad Shuaib Khan3, Shakeeb Ullah3
Ali Zaman3, Kamran Safdar3, Khalid Muhammad3, Ambrina Tariq4
Muhammad Inam Malik3, Muhammad Kamal Shah3, Atiq ur Rehman3 
and Hamza Maris3
...ivestock but potentially satisfactory alternative technique is the immunological control against ticks infestation. Therefore, this study was planned to investigate the effect of crude extract of hard ticks and its application as a vaccine against hard ticks of the cattle. For this purpose, ticks were collected from cattle and crude extract of ticks was prepared using standard procedures i.e. mixed with formalin, antibiotic and vegetable oil to prepare ...
Ayman Balla Mustafa1, Abdalla Elgenaidi1, Aldukali Alkeskas1, Asim Faraz2,*, Ahmed Eisa Elhag3, Mohanad Bashari4 and Bernard Faye5
... managed together at the same environmental conditions under intensive system. Collection of milk samples started at second week postpartum and continued biweekly interval up to end of 4th month postpartum. The total fat, protein, lactose, solid non-fat (SNF) and density percentages were determined by automatic milk analyzer device (lactoscan Model-90, Europe). Results elucidated that there was no significant diff...
Liping Liu and Pengfei Liu*
...ging together closely on same substrates in one flock during non-breeding season.

...
Jun Yan Bai*, Zhi Hao Dong, Shu Juan Zhao, Ying Lei, You Bing Yang, Heng Cao and Kun Peng Shi
...nch giant meat quail and savimalt meat quail in order to provide reference for breeding and improvement of meat quail. The results show that french giant meat quail shows far higher body weight, carcass weight, carcass net weight, breast muscle weight (total), leg muscle weight (single) and leg muscles rate compared to savimalt meat quail (P<0.05). Moreover, heart weight, liver weight, slaughter rate, whole net car...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...spitals of Lahore and Faisalabad,Pakistan. A total of 148 A. baumannii isolates were collected. The initial identification of bacterial isolates was performed by standard microbiological techniques, and API 20E and for final confirmation multiplex PCR was performed using genus and species-specific primers. The susceptibility to various antimicrobial agents including the aminoglycosides was determined by the Kirby-Bauer method and minimum inhibitory conc...

 Yanqiang Zhou1, Lixin Wang1, Chunxiao Hao1, Xiuyun Li2, Shakeel Hussain1, Dongdong Shen1, Zhiwei Peng1, Qi`an Zhai1 and Zhijun Hou1,*

... alpacas, and goats with same husbandry and fence site in Harbin Zoo, China. From August 2015 to August 2016, 507 fecal samples of blue wildebeest (188), alpacas (195) and goats (124) were examined for gastrointestinal parasites by saturated sodium chloride floatation technique. The microscopic analysis, based on the morphology of oocysts, indicates that the present parasites are Capill...
Niaz Muhammad1, Samina Yasin1, Zunaira Fatima1, Noor ul Ain1, 
Muhammad Faizan2 and Sadaf Naz1*

Fahima Khatun1, Abdullah-Al-Maruf2, Md. Mizanur Rahman2, Afroja Yasmin1, Mohammad Ali Zinnah3, Md. Aminul Islam4 and Mohammad Shah Alam5*

...als for treatment. Fecal samples were collected from the rectum and examined by direct smear method and helminths identified by the presence of characteristic eggs in the feces. This study was carried out with three age groups: calves (<1 year), young (1-3 years), and adult (>3 years) and three different consecutive seasons (winter, summer, and rainy) during the periods of January 2018 to December 2018. The highest incidence was found in infestation with...
Waqar Ali1,3*, Muhammad Yaqoob2, Abida Arshad1, Guifu Dai3,Safir Ullah Khan1, Muhammad Arshad Malik4, Imran Ullah1 and Ihsan Ullah1
...ll;">A total of 514 milk samples collected independently from beetal goats were tested for prevalence of clinical and subclinical mastitis through surf field mastitis test. The bacterial isolates identified through different biochemical test and colony morphology from positive milk sample were Staphylococcus aureus (25.4%), Escherichia coli (20.3%), Streptococcus (16.9%) in which (6.7%) Streptococc...
Yuli Andriani1*, Rintan Octaviana Julia1, Lintang Permata Sari Yuliadi1, Iskandar Iskandar1 and 
Yaya Rukayadi2
...e result indicated that usage of BPLM is safe as a sources of natural pigment for ornamental fishes, such as Swordtail fish.
...

Muhammad Aftab1*, Aneela Riaz2, Ghulam Sarwar3, Muhammad Arif1, Qudsia Nazir1, Ifra Saleem1, Sarfraz Hussain1, Abid Ali4, Abid Niaz1, Fakhar Mujeeb4, Khalid Mahmood5 and Sarfraz Nawaz6

...the mechanism involve in salinity-boron interaction is still not well known. This research was carried out to evaluate the plants tolerance under combined stress of B and salt. In combined stresses of (B and Salt) causes oxidative stress in plants due to increase in production of the reactive oxygen species (ROS) including hydroxyl radicals (OH-), hydrogen peroxide (H2O2) and superoxide (O2-). However, on their side, efficie...

Hidayatullah1*, Sammia Mahroof1, Saleem Abid2, Naveeda Anjum3, Noor Habib4, Akhter Saeed5 and Muhammad Arshad Farooq1

...r locations; Chakwal, Faisalabad, Islamabad and Swat. Combined analysis of variance showed that mean yield of chilli advance lines at four locations were statistically significant. Genotype x locaton had significant interaction suggesting inconsistent performance of chilli genotypes over environments. The NARC Chilli-2 has regression slope equal to one with relatively low Wricke’s Ecovalence, low value of Shukla’s Stability Variance, relatively les...

Muhammad Zubair Ishaq1, Umar Farooq1, Muhammad Asim Bhutta2*, Saghir Ahmad2, Amna Bibi2, Hafeez UR Rehman3, Umar Farooq3, Javaria Ashraf4 and Samaria Nisar4

Diana Rachmawati1*, Johannes Hutabarat1, Olga Anne2, Roy Hendroko Setyobudi3 and Tita Elfitasari1
.... fuscoguttatus. The sampled fish has an average weight of 4.24 g ± 0.023 g per fish. Diet used in the study contained 45 % protein with the supplementation of various amounts of B. subtilis varying in (0, 2.5, 5, 7.5, 10, and 12.5) % per kg of diet as A, B, C, D, E, and F treatment, respectively. The results show that B. subtilis supplementation in the diet significantly affected on protein digestibility (ADCP), the efficie...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...miptera) are destructive sap-sucking pests of citrus and cotton, respectively. Extensive use of persistent synthetic insecticides against these pests poses issues of environmental contaminations and health hazards and suggests looking for alternate biorational plant protection measures such as botanical pesticides. This study evaluated the potential toxicity of acetone extracts of 40 indigenous plant species of Soon valley and surrounding
Ayu Suci Wulandari1, Siwi Indarti1,2*, Muhannad Illayan Massadeh3 and Nguyen Van Minh4
...sts in garlic (Allium sativum L.). To determine the influence of elevation and soil abiotic factor to abundance and diversity of plant parasitic nematode in garlic crops, this research was conducted Sampling  was carried out in four locations: Brebes, Magelang, Tegal and Temanggung, Central Java, Indonesia. Samples of soil, roots and tubers were collected. Soil abiotic factors are taken measurements such as pH, temperature, and organic matter. The ...
Ahmad Wahyudi1*, Sujono Sujono2, Listiari Hendraningsih2, Ari Prima2, Zane Vincēviča-Gaile3 and 
Ivar Zekker4
...DM and OM (%) showed the same pattern, TMR followed by CON then TMR silage (64.09; 62.98; 54.21 for DMD) and 73.92; 75.99; 63.78 for OMD. The age has non-significant effect into digestibility. Highest ADG (kg head-1 day-1) were was obtained from TMR feed (0.70), followed by CON (0.65) and TMR silages (0.45). The older calves the higher ADG reached (0.65; 0.60 and 0.55). It could be concluded that addition urea 1.5 % in TMR could increase ...
Naveed Farah1*, Izhar Ahmad Khan1, Asif Ali Abro2, Jehanzeb Masood Cheema3 and Muhammad Luqman4*
...itudinal analysis of Landsat imagery of land use was done through GIS and Remote Sensing for a time period of 2001-2016. The results of GIS analysis revealed that during the period 2001 to 2016, Faisalabad showed 24% increase in urban area and 23 % of this expansion consumed agricultural land. In Sahiwal, urban land has increased by 21 %, which encroached 13 % of agricultural land and 8 % bare land. Qualitative and quantitat...

Rahmat Gul1*, Imran Khan1, Iftikhar Alam2, Zia Uddin1 and Zafar Iqbal3

...f Ramadan in 2018 from a sample of 109 (majority men; 60.5%) . Based on these data, using the frequency of report and on the contribution to the intake of energy and nutrients in the 24-HDR, 80 distinct food items were identified that are commonly used during Ramadan. Respective reference servings of these food items were selected to comprise the Food Frequency Questionnaire  food list. The most reported portion sizes in the 24-HDR were used for Reference...

Usman Khalique*, Muhammad Zahid, Noor Fatima, Syed Jawad Ahmad Shah

...ams. Further, it is necessary to identify the resistant genes in these varieties that could be incorporated in future wheat cultivars.

...
Nazish Huma Khan1*, Mohammad Nafees2, Tooba Saeed3, Khaliq Ur Rahman4 and Adila Bashir2
 
...ling, treatment and disposal of urban and industrial solid wastes. For this reason, there are difficulties in planning solid waste management. In this context, this study aims to determine the organization’s role in record keeping and solid waste management in the Hayatabad Industrial Zone, Peshawar. To this end, a questionnaire survey was carried out among 105 industries and relevant government departments were interviewed. The solid waste generation ra...

Muhammad Dawood1,2, Naeem Sarwar2, Muhammad Umer Chattha1, Imran Khan1, Muhammad Bilal Chattha3*, Faiz Hussain4, Muhammad Mahmood Iqbal5, Muhammad Akram4, Hammad Husnain5, Muhammad Shahid5, Mussarrat Hussain6 and Muhammad Umair Hassan1

...ial and quality under Faisalabad conditions. The experiment was comprised of eight wheat cultivars, i.e. Galaxy-2013, Ujala-2015, Inqlab-2000, SH-2002, Punjab-2011, Millet-2011, Faisalabad-2008 and Lasani-2008. The results revealed that all wheat cultivars have differential response for growth, yield and quality. The wheat cultivar Galaxy-2013, produced the maximum productive tillers (298 ...

Muhammad Yaseen*, Muhammad Adnan, Muhammad Luqman, Muhammad Zeeshan Majeed and Muhammad Umer Mehmood

...lation of rural youth, a sample of 450 respondents was selected using a multistage sampling technique. Statistical Package for Social Sciences (SPSS) was used to have descriptive statistics of data. The majority of youth respondents were taking part in agricultural activities like land preparation, sowing practices, irrigation, harvesting and storage obtaining a percentage of 80.2%, 77.6%, 79.3%, 90%, and 75.8% respectively....

Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali

...and DSS solutions of egg-sac silk exhibited no inhibitory potential by either of the two methods. However, application of web silk using the DSS treated discs showed no growth inhibition compared to the DgS treated discs, where increased inhibition zones were observed against all three bacteria. This study suggested that spider silk does possess antibacterial potential, however, this is not an intrinsic property and is type specific as well as species specific...

Hasnain Raza1*, Maryam Maqsood2, Muhammad Muzamil Nazir1, Iqra Tariq1, Kaynat Ahmed1, Qurat-ul-Ain1, Ali Raza2, Huda Bilal3, Muhammad Bilal Shoukat1, Attiq ur Rehman1, Awais Rasheed1 and Muhammad Zeshan Gulzar1

... solutions to this universal problem are being provided through eco-technologies, such as constructed wetlands (CWs). CWs are among the most widely used natural water management options. CWs can be used for phytoremediation, which acts as a natural sink for toxins. The present paper aims to provide a brief discussion on wastewater health risks, CWs, and its phytoremediation attributes as a plant-based cleanup solution for wastewater remediation.

...

Junaid Ahmad1, Shahzeb Javed2*, Mehmood Khan1, Shadman1, Muhammad Farooq3*, Muhammad Saleem4, Rahila Afzal5, Muhammad Aasim6, Naqeeb Ullah7, Muhammad Noman Khan8, Dawood8 and Naila Ilyas9

...xt-align: justify;">Food safety and cleanliness are becoming increasingly important in Pakistan, as new incidences of food contaminants emerge, which, if overlooked, can result in a variety of health risks, illness, outbreaks, and even death. The goal of this study was to look at the students’ knowledge, attitudes, and practices on food safety and cleanliness at a different institution in KPK, Pakistan. A cross-section...

Aiman Amur1*, Nasreen Memon1, Pervaiz Khan2 and Fozia Gull3

...ruits. The Bactrocera dorsalis is one of the complex fruit flies of this diver’s genius, hunting on very delicious fruits such as mango. The purpose of this study is to observe the relationship among the environmental and host effects on the outbreak of this crucial pest. The research work was conducted in two gardens (50 hectors) of mango fields of Mirpur Khas, known as mango land of Sindh during (2014-2015) seasons. The two sites of garden with differe...
Mona Mohammed EL-Derbawy1, Eman Naser Hafez2*, 
Mona Abdel Daym Abd Rabbo1, Nagwa Ibrahim Toaleb3,
 Saedia Abdel Hady Sayed El-Ahl1 and Bahaa Eldkeen Wade EL-Aswad4 
...ifty two individual sera samples divided as follows: Group І included 21 obtained from patients who were positive for schistosomiasis and negative for other parasitic infections; Group ІІ included 18 sera from patients who were negative for schistosomiasis and positive for other parasitic infections; (3) fascioliasis, (5) amoebiasis, (7) toxoplasmosis and (3) hydatidosis and Group ІІІ included (13) sera from healthy individuals all were diagnosed parasit...
Aneela Hameed1, Faqir Muhammad Anjum2, Zia ur Rehman3, Saeed Akhtar1, Asim Faraz4*, Majid Hussain1 and Amir Ismail1
...e lactation stages, milk samples were collected from two groups of sixteen animals each under controlled atmosphere and feeding conditions. One group was subjected to intramuscular injection of oxytocin (20 IU) and other was kept as control. Significant variations were obtained in milk composition along with lactations stages. Decrease in fat, protein, lactose, solids not fat and total solids contents and increase in ash contents were noted in oxytocin adminis...
Jun-Dong Tian1,2, Wei-Jie Guo1,2, Han Yan1, Jie Zhang1, Shu-Liao Tian3 and Ji-Qi Lu1,2,*
...ta, we used non-invasive sampling method to collect the feces defecated in the morning (AM group) and afternoon (PM group) from a zoo rhesus macaque population. Then 16S rRNA sequencing technology was adopted to assess the microbial communities. The results showed i) the dominant phyla were Firmicutes, Bacteroidetes, Proteobacteria and Spirochaetace accounting for over 86 % of the richness of whole microbiota, ii) Ruminococcaceae, Prevotellaceae, Lactobacillac...
Muhammad Mansha1*, Sidra Yaqub1, Asma Abdul Latif2, Muhammad Wasim3 and Peter C. Thomson4
Anwar Khan1, Fareeha Nosheen2,4, Bushra Tabassum2*, Olawale Samuel Adeyinka2, Khurram Shehzad3, Naila Shahid2, Arif Muhammad Khan4 and Idrees Ahmad Nasir2
...irus X in cell culture assays. Three specific siRNAs against CP-PVX gene were designed in which siRNA 3 significantly reduced the CP-PVX mRNA expression in HepG 2 cells upto 91.91% (12.5 fold) as compared to siRNA 1 and siRNA 2 where knockdown percentages were only 20 and 73.04%, respectively as revealed by realtime PCR. Maximum knockdown values were obtained at 100 nM concentration of siRNA 1 and 2, while for siRNA 3 promising knockdown was obtained at 50 nM ...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...rospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. Haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte an...
Meiping Deng1, Xiaowen Ye1, Jiashan Wu1, Lijuan Chen1, Xiaoxia Jiang1, Changzheng Zhang1, Peiling Zhou1* and Yi Luo2*
...rats were anaesthetized, saline or arecoline was intravenously administrated, and the systemic BP signals were recorded. We calculated the reaction times, the mean arterial pressure (MAP), the maximum changes in MAP, and the area under the curve (AUC; MAP change relative to the reaction time) due to arecoline stimulations. The results showed that arecoline induced biphasic modulations in BP, including an initial downregulation (Period 1) and a subsequent upreg...
Amal E. Abdel-Hady*, Sara M. El-Amir, Noura S. Al-Muttri and Heba M. El-Hariri
... outer surface of the dorsal scales from the mid-body region of Acanthodactylus opheodurus (Lacertidae), Mesalina guttulata guttulata (Lacertidae) and Trapelus ruderatus blanfordi (Agamidae). Skin specimens were prepared and investigated by using scanning electron microscopy. The microornamenation of the examined species exhibited different pattern in the same microhab...
Essam H. Ibrahim1,2,3, Attalla F. El-kott1,4, Ali Alshehri1, Mona Kilany2,5,  Reza Yavari6, Salahud Din7* and Diaa Massoud8,9
... were observed on the dorsaum lingua. Numerous filiform papillae were visible on the lingual apex and body, while, fungiform papillae were scattered among them. The discoid-shaped fungiform papillae were more densely on the lingual apex. The filiform papillae were extended to the lateral margin of the lingual body. They were transformed into the conical papillae on the caudal part of the lingual body and root. Totally, 14-16 of spheroid and oval vallate papill...

 Song Jiang1,2, Xianbin Mo1,2, Falin Zhou2,3, Jianhua Huang2,3, Qibin Yang2,3, Lishi Yang2,3 and Shigui Jiang2,3*

...on families fed with the same diet(P<0.05). The AGR (absolute weight gain rate) of the fastest weight gain families was 97.16% and 95.46% higher than that of the slowest weight gain families fed with Diet A and Diet B, respectively, but the order of weight gain of different families in the different diet groups was not the same. The average survival rates of P. monodon fed with different diets were significantly different...
Yonghua Liu*, Xianhua Li, Xiongfei Yan, Gang Li, Caiyun Luo and Ying He
... treatment times, at the same temperatures, the average fecundity, fecundity rate, and average adult longevity (of both the males and females), also gradually decreased.In conclusion, T. vishnou gigantina is significantly impacted on survival and reproduction by short term high temperatures. This study is expected to provide a scientific basis for predicting the population dynamics in T. vishnou gigantina.
...

Suhu Duan, Zaiqiang Li, Zhenzhen Fan, Mengran Qin, Xiaoxue Yu* and Liuan Li*

...illa) seeds on the polyunsaturated fatty acid in egg yolk. Three groups, each of 40 healthy Gallus domesticuss 26 weeks old, were fed 6, 10 and 15% of perilla seeds in addition to daily basic ration.. The control group (CK) of 40 birds was fed basic daily ration. The polyunsaturated fatty acid (PUFA) content and the ratio of n-6/n-3 PUFA in yolk were determined by gas chromatography with 24 eggs randomly selected from...
Dilek Şahin1*, Meryem ÖZ2, Orhan Aral2, Mehmet Bahtiyar2 and Selda Taşçi2
...owth rate and feed conversation ratio values occurred at 0.815 g, 0.462 % and 0.86, respectively were observed in the group fed with 9% of purslane extract. The survival rate parameters indicated no death in any experimental group of goldfish fry. The purslane extract had a positive effect on coloring, and the highest pigmentation for goldfish was obtained in T6 and T9 (Hue (Habº) angle values (86.73±0.32 and 77.64±0.47, respectively). The r...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
...ve real-time (qRT)-PCR assays were performed to explore the effects of QZZKF on the expression of surfactant protein A (SP-A), B7 homolog 3 protein (B7-H3), interleukin (IL)-12 and IL-13. The expression levels of B7-H3 and IL-13 were upregulated after MPP infection in the mouse model (P<0.05), while the expressions of SP-A and IL-12 were significantly decreased (P<0.05). After five days of therapy, the protein and mRNA expression levels of B7-H3 and IL-1...
Jialiang Han1, Guohou Liu1*, Wenke Bai2,3, Qixian Zou4, Ye Cao5, Caiquan Zhou2,3*, and Guy Michael Williams6
Muhammad Arslan Akbar1, Khalid Javed2, Asim Faraz3* and Abdul Waheed3
...(TL), barrel depth (BD), sacral pelvic width (SPW), birth weight (BiW), Live body weight (BW), teat length (TEL), teat diameter (TED), testes length (TsL), testes width (TsW) and scrotal circumference (ScD). Male and female animals were placed in two separate groups. The correlation coefficients among most of the morphometric traits were high and significant (P≤ 0.01) particularly among withers height, body length, heart girth and live body weight in both m...

Milkessa Wakjira, Fikiru Temesgen*, Chala Hailu and Dabesa Wegari

...f data. Three multistage sampling techniques was employ to select 372 MSE in six district towns of Western Shewa Zone by administering personal interview. Descriptive statistics such as percentage, frequency, maximum, minimum, mean and standard deviation were used to measure the association and growth probability of MSEs. The major problems finding in the study area were facing multiple issues such as increase in cost of production, shortage of workers and red...

Kemi Funmilayo Omotesho1*, Philip Akintunde Fatodu1 and Toyin Benedict Ajibade2

...ng. A three-stage random sampling technique was used to select 178 youth farmers on whom a structured interview schedule was administered. Data was analyzed using descriptive statistics and the Multiple Regression Analysis. Results reveal that most of the respondents were male (73.6%), with mean age of 28 years and average of 12 years farming experience. Farmers’ level of record keeping was low (Mean=2.26), and their attitude was poor (index=0.43). Farme...

Malik Muhammad Shafi, Rabia Habib and Haidar Ali*

...affecting farmers’ savings the study was designed in district Kohat (KP, Pakistan). For the purpose of data collection, interview schedule and direct observation was used as a research tools. Through random sampling technique, a sample size of 90 households from 452 farm households were taken. Total consumption expenditures, total income, and land size were the variables which showed...

Shahid Ali1*, Murtaza1, Waqas Ahmad1, Muhammad Israr2, Aftab Khan3, Hamdullah1 and Syed Attaullah Shah1

...khwa, Pakistan. A random sample of 275 rice growers was selected from district Swabi, using a multi-stage random sampling procedure. Pre-tested interview schedule was used for collection of data from rice growers. Through Cobb-Douglas type production function (CDPF) data was analyzed. Results indicated that 1 percent increase in labor hours and urea increased yield by 0.411% and 0.231%, respectively, at 0.01 level of α...

Theophilus Miebi Gbigbi* and Rodney Akpoviri Isiorhovoja

...e, Nigeria. A multistage sampling procedure was adopted to pick a sample of 120 farmers from whom data were elicited using structured questionnaire. Data were analyzed using descriptive and inferential statistics. The findings indicate that the average age of respondents was 43 years; majority (85%) of respondents were males with 16 years’ experience in exotic chicken production; 75% were married and 48% had secondary ...

Saadu Mustapha1,2, Norsida Man1*,  Jasmin Arif Shah1, Nitty Hirawaty Kamarulzaman1 and Ahmadu Abubakar Tafida3

...sing multi-stage cluster sampling technique, using organized questionnaires administered to 254 respondents. Descriptive and inferential statistics was used. The research used Rogers’ Theory of Innovation Diffusion, Rogers and Burde’s Theory of Social Change. The findings from the study have shown that GSM, radio, television, camera, internet, computer, CD/DVD player and VHS video are the most commonly used ICT components in various aspect of exten...

Nazeer Ahmed Lashari1*, Saghir Ahmed Sheikh2, Aijaz Hussain Soomro2 and ShamsuddinTunio3

...rough stake Sheller, the samples were dried at 25±2 °C for 24 hours and kept in cotton, gunny & nylon bags and stored for the period of one year at 20 ± 1°C in cold storage room. The current findings indicates that the maximum moisture content was 20% of (DR-82) in nylon packing at 6 months of storing time; whereas, lowest moisture was observed in BS-385 (10.76%) at fresh level. 1000 grain weight (g) was between 14.90g to 25.00g for a...

Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1

... was accomplished at the same reaction conditions. Amplified products had a length of 400 bp, which was our estimated length. After the sequencing of these amplicons, In silico restriction analysis was performed for each specie. Restriction endonuclease TfiI was selected for further analysis. Restriction fragment length polymorphism (RFLP) was performed at the same reaction conditions for all species. RFLP analysis produced ...

Raheela Noor Memon1*, Nadir Ali Birmani1, Naeem Tariq Narejo2 and Shahnaz Rashid3

...ary 2020. A total of 107 samples of Cyprinuscarpio were tested during the research and helminths were found in 17 out of 107. Various helminths groups were identified including trematode and acanthocephalan. Other helminths groups, such as cestodes and nematodes were not noted. These helminths infected the gut of Cyprinuscarpio including trematode and acanthocephalan. No additional organs were found to be infected with helminths parasites. Helminths prevalence...

Murad Ali* and Said Wahab

...ored throughout. All the samples were analyzed for TPM and Acid value at the time of collection with the help of oil testometer DOM-24. A 100 ml of sample from each of the frying mediums was collected and transferred to the Biochemistry Laboratory, University of Malakand for further analysis. The peroxide values (18.073 meq kg-1) were found significantly higher in oil medium, followed by ghee (11.96 meq kg-1) and then tallow...

Kirshan Chand1, Fahad Nazir Khoso1*, Arfan Ahmed Gilal1, Abdul Mubeen Lodhi2, Agha Mushtaque Ahmed1, Ghulam Murtaza Jamro3, Sohail Ahmed Otho1 and Jamal-U-Din Hajano4

...route, mode of action, dosage, calibration and application timing) but one factor that doesn’t get much attention is the quality of the water used to spray the product which may reflect in the success of spray operation. To know the influence of water quality on pesticide performance, this study was proposed to evaluate the water sources used for mixing the insecticides prior to application. For this purpose, two districts of lower Sindh province i.e., H...

Muhammad Yaseen1*, Muhammad Sallam Shahzad2, Farhat Ullah Khan2, Muhammad Luqman1, Usman Saleem3 and Shoaib Nasir4

... District. A multi-stage sampling technique was used for this study; firstly, one tehsil out of three tehsils i.e. Narowal, Shakargarh, and Zafarwal was selected, secondly, 6 union councils were selected, and then two villages from each union council and 10 farmers from each village were selected randomly. Thus, a total sample comprised of 120 rice growers. A well-structured interview schedule was used for data collection. D...
Zafer Karslı1,*, Dilek Şahin1, Meryem Öz2, Ünal Öz2 and Orhan Aral2
...itive (growth, sex conversation etc.) impacts of hormone administration were also assessed. This study had two experiments. In the first experiment, six experimental feeds were prepared by adding two different hormones (17α-Methyltestosterone (Mt), 17β-Estradiol (Es) at three different doses (20, 40, 60 mg kg-1) and control feed was without hormone. All six experiment groups receiving experimental diets and control were conducted i...
Dia Mamadou1,2*, Meissa Beyah1, Sow Amadou Harouna1, Ba Samba Alassane1, Bouzouma Moustapha1, Braham Cheikh Baye1 and Beibou Ely1
...es a week or even daily, sampling was carried out on board the vessels or at lobster production plants of this species at the time of their sorting and packaging. A total of 31770 individuals were measured and weighed; their level of maturity and moulting state were noted. This deep-sea species, reproduces all year round with a period of intense reproduction between August and December. The size of the smallest mature female mature female encountered during th...
Muhammad Zahid1, Aneela Zameer Durrani1, Muhammad Ijaz1, Khushi Muhammad2,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal3
...luated on the basis of disappearance of clinical biomarkers, while values of BEF positive animals’ hematological and serum biochemical parameters were analyzed and compared with altered values in different conditions. These physiological biomarkers were evaluated at short intervals and processed for total MCV, MCH, MCHC, and lymphocytic and leukocyte count, most importantly. It was recorded that there was significant increase in number of WBCs and neutro...
Amin Arif1, Khizar Samiullah1*, Riffat Yasin2, Bilal Rasool1, Shakila Naz1, Xijun Ni3 and Saleem Akhtar1
...als the persistence of mosaics of diverse habitats ranging from tropical evergreen forest to subtropical ones, closed seasonal woodlands to wooded savannas during the deposition of the Dhok Bun Ameer Khatoon, Chinji Formation.
...
Madiha Nawaz and Shoaib Freed


...Ma-11.1, Ma-2.1) and Isaria fumosorosea (isolates If-2.3, If-02) showed percent mortalities of (61.0%, 85.0%), (78.0%, 56.0%) and (52.0%, 54.0%) with LC50 values of (4.25×108, 2.54×108 spores/ml), (3.26×108, 5.08×108 spores/ml) and (6.22×108, 7.20×108 spores/ml) with LT50 (6.43, 4.80), (5.43, 6.74) and (6.66, 6.69) days at highest ...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
...c stress affects lipopolysaccharide (LPS) -induced inflammation via the crosstalk. Pigs were administered LPS injection with or without continuous adrenocorticotropine hormone (ACTH) pretreatment. Twenty-four 30-day-old Duroc × Landrace × Large White crossbred piglets (12 ± 0.5 kg) were randomly assigned to 4 treatments: control, LPS, ACTH, and ACTH+LPS groups. Each group consisted of 6 male piglets. All ACTH pigs and ACTH+LPS pigs were inje...

Ilker Kepenekci1, F. Dolunay Erdoğuş2 and Adnan Tülek3*

...PPNs from soil and plant samples collected from strawberry orchards in 10 different locations in Tokmok area of northern Kyrgyzstan during the summer of 2016. PPNs were identified according to morphological and morphometric characters. Eight species belonging to the orders Tylenchida and Aphelenchida (Nematoda) were identified: Bitylenchus dubius, Boleodorus thylactus, Filenchus filiformis, Helicotylenchus digonicus, Merlinius brevidens, Pratylenchus neglectus...

Christopher Oche Eche1*, Juliana Iye Oluwatayo1, Demben Moses Esang2, Paul Madina2 and Alexander Uloko1

...nvestigate the effect of sawdust mixed with eucalyptus biochar on nematodes associated with beniseed as well as their impact on yield. A total of nine treatments combinations were evaluated using a 3 x 3 factorial experiment laid out in a Randomized Complete Block Design (RCBD) in three replications. The treatments combinations consisted of single applications of sawdust and eucalyptus biochar, and mixed formulations of

Yonis Abukar Mohamed1, Shafii Abdullahi Mohamed1,2, Abdiaziz Idiris Mohamud1,3*, Abdiaziz Ahmed Mohamud1,3, Kassim Abdullahi Jimale1,2 and Said Ali Ibrahim2

Thomby Paul, Sreekanta Biswas, Sabiha Zarin Tasnim Bristi, Debashish Sarker, Saroj Kumar Yadav and Bhajan Chandra Das*

Riaz Alam1*, Muhammad Sajid2 and Durri Shahwar3

...of dropper. The daughter saplings of cultivar Manzanilla produced through air-layering by the application of IBA in various concentrations, produced early rooting (36.00 days), high rooting percentage (77.47%), maximum number of roots (6.38) per layering, root length (7.03 cm), root weight (2.62 g) and more number of re-sprouts (5.57), followed by number of days to root appearance (38.80), rooting percentage (65.73%), number of roots (5.69) and root length (6....

Muhammad Junaid Yousaf 1, Farhad Ali2*and Fawad Ali2

...is considered a moderate salt-tolerant plant cultivar that shows varying effects and adaptations to different concentrations of salt stress (i.e. NaCl) to which it is exposed. When three days old seedlings of maize were subjected to aerial salt stress of concentrations; 0.2%, 0.6% 1.2%, and 2% after a week in soil, the seedlings revealed the antagonistic effects which increased with elevat...
Ziyue Qin1, Zhen Wang1, Ali Mujtaba Shah3,4, Zifan Ning1, Yaofu Tian1, Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Lin Ye1, Diyan Li1, Gang Shu2 and Xiaoling Zhao1,*
...etic background have the same response to feed restriction is not reported, herein we evaluated three levels of feed restriction effects on growth performance and blood glucose concentrations in faster- and slower- growing chicken (different genotypes). In the present study, birds were restricted to 100% to full feed in faster- and slower- growing broilers groups. Body weight gain, feed consumption, blood glucose concentrations were measured on each week from ...
Qazi Muhammad Noman1, Farhan Mahmood Shah1, Khalid Mahmood2 and Muhammad Razaq1*
...l group) and static Spinosad® (spinosad 2% and methyl eugenol 51%, target group) in mango and citrus orchards at different locations in Multan, Southern Punjab, Pakistan. Bactrocera zonata (Saunders) was the most abundant fruit fly species in this study. Our results indicated a positive and significant correlation between the species of fruit flies and mean temperature in mango and citrus orchards at al...
Song Jiang, Dong-liang Liu, Fa-lin Zhou, Xian-bin Mo, Qi-bin Yang, Jian-hua Huang, Li-shi Yang and Shi-gui Jiang*
...y up-regulated under low salt stimulation (P> 0.05),while under high salinity stimulation, the transcription level of PmATP synthase mRNA reached the maximum at 16th h (P<0.05).The expression level of PmNa+-K+-ATPase mRNA increased at 4th h and 16th h, which was significantly higher than that in the beginning under low salt stimulatio...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...s research a total of 79 samples were collected from different local market. In which 54 samples were from dried fish and 25 from cooked fish samples. In this research there were 18 different types of dried fish and 6 types of cooked fish were used as a sample. Laboratory work was done by different bacteriological laboratory methods and purified isolates...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
... of oocyte maturation, disappeared after parthenogenetic activation, re-appeared and elevated in the blastocysts stage. The following western blot analysis revealed a similar expression pattern with QRT-PCR and immunofluorescence. In conclusion, the expression pattern of Gas6 gene is closely related to oocytes maturation and embryo development. Gas6 may play a crucial role in the process of oocytes maturation and improve the development potential of ear...
Manar M. Farouk1*, Amal El-Molla1, Fayez A. Salib1 and Yousef A. Soliman2
...d with the occurrence of salmonellosis in some diarrheic sheep and goats belonging to mixed reared flocks in Giza governorate, Egypt, 2) Investigate the presence of enterotoxin(stn) gene in the recovered Salmonella spp. strains, and 3) Build a phylogenetic tree for the partial codon sequence of stn gene of some recovered strains in order to provide a scientific basis for the implementation of practical preventive measures. A total number o...
Muhammad Awais1,2, Waqas Ahmad3, Akhtar Rasool Asif4, Sayyed Aun Muhammad1, Iahtasham Khan1*, Muhammad Amjad Ali5, Muhammad Qaiser Riaz1 and Muhammad Saqib2 
...with pyrogen free normal saline, 0.05% PI gel, certified Acacia honey and H-PImxr (an equal volume mixture of Acacia honey + Pyodine gel) respectively. Significant differences were found among four treatments with respect to contraction, granulation tissue, tensile strength, healing time and hydroxyproline contents measurement in both experiments. Honey and povidone mixture treated animals showed significantly improved wound contraction, healing time, t...
Ikechukwu Eugene Onah*, Ifeoma Esther Aniaku, Jude Ifeanyi Okwor and Ada Sylvester Haruna
...t of crow-man contact to safeguard the public health of rural and urban dwellers in the study area.
...
Muhammad Hamza Tariq1, Mahjabeen Akram2 and Yasir Sharif3*
...pting SwissADME and ADMETsar online servers. The compounds namely Morin, Curcumin, Apigenin, Cedronolactone A and Matairesinol showed acceptable drug-like properties therefore these compounds can be proposed as effective inhibitors, disrupting the S-protein- ACE2 interaction. This study might help in the development of a natural and cost-effective drug against COVID-19. Further, in vivo and in vitro examinations are required to validate our resul...

Muhammad Usman Asif*, Raza Muhammad, Waseem Akbar, Mubasshir Sohail, Muhammad Awais and Javed Asgher Tariq

Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...ons, their frequency, consanguinity in parents and inheritance pattern in patients of Karak region, Khyber Pakhunkhwa (KP) province, Pakistan. During the study, 200 peripheral blood samples were collected both from families having at least one transfusion dependent child and sporadic patients from different areas of karak region. Using the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) techniqu...

Amina, Muhammad Zahid Rashid and Amina Rashid

...stigate the role of chitosan coating on partially mature guava to retain good quality and prolong shelf life in cold (11°C). Different chitosan concentrations i.e. 20% 30% and 40% were coated and stored at 11°C temperature. These fruits were compared with control (non-treated) fruit after 15 days. It was observed that the fruits coated with 40% chitosan showed minimum weight losses...
Zhi Hao Dong1, Jun Yan Bai1*, Xiao Ping Jia2, Ying Lei1, Hui Cao1, You Bing Yang1 and Guang Lu Li1
...n french giant quail and savimit quail (0.531 and 0.778, respectively). The frequencies of TT genotype in expressed exon 4 of ESR gene were the highest in french giant quail and savimit quail (0.338 and 0.581, respectively). The frequencies of TT genotype in expressed exon 8 of ESR gene were the highest in french giant quail and savimit quail (0.544 and 0.838, respectively). ...

Dilshad Ahmad1*, Muhammad Afzal2 and Asif Ali Abro3

...study used simple random sampling approach for collecting primary data of 240 livestock household farmers and employed regression analysis model to estimate credit impact on household income and livestock production. An economic technique of log-linear regression model was applied to identify the factors influencing dairy production and estimate household income difference from dairy production due to formal credit from Zarai Taraqiati Bank Limited (ZTBL). Est...

Mubeen Zahra1,2, Muhammad Nawaz3, Muhammad Umer Chattha4, Imran Khan4, Muhammad Bilal Chattha5*, Muhammad Ashraf Bhatti6, Abdul Rehman4, Faryal Ahmed4, Faran Muhammad4, Mina Kharal7 and Muhammad Umair Hassan4

...) and wheat cultivars Faisalabad-2008 (standard height and low tillering), Td-1 (low height and low tillering) and Galaxy-2013 (standard height and high tillering). The results indicated that maximum leaf area index (LAI), crop growth rate (CGR), tillers (356.2 m2), spikelet/spike (18.61), grains/spike (49.53), 1000-grain weight (44.4 g), biological yield (12.95 t ha-1) and grain yield (4.61 t ha-1) was recorded in control whereas lowest value for these parame...

Muhammad Usman Hanif, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad, Muhammad Irfan Ullah

...in benzoate 1.9 EC, spinosad 240 EC and lufenuron 50 EC) and the aqueous extracts of three local plant species (i.e. Eucalyptus camaldulensis Dehn., Citrus limon L. and Azadirachta indica A. Juss) against the grubs and adults of E. vigintioctopunctata. Three concentrations of each synthetic insecticide (i.e. 2.5, 2 and 1.5%) and botanical extract (i.e. 10, 7.5 and 5%) were bioassayed using standard leaf-dip method. Results r...

Ayesha Khan1*, Asmatullah2, Urooba Perviaz1, Khalid Nawab1 and Mahmood Iqbal1

...ermenter on fertilizers usage and irrigation of selected crops. Organic farming is the dire need of the day; to overcome the excessive use of chemical fertilizers alternative methods are adopted to decrease use of fertilizers. One of the techniques for organic farming is EM-Technology. EM-Technology is used in addition with animal manure and other organic wastes effectively in agriculture. The universe of the study was Malakand division of Khyber Pakhtunkhwa. ...

Sonam Khatri1, Shaheen Faizi2, Shahina Fayyaz1 and Erum Iqbal1*

...ent of an alternative biosafe measure to control these pests. In this regards, researchers have focused on the use of phytopesticides which are eco-friendly and easily accessible and degradable in soil as compared to synthetic chemcials. The given information in this review article highlights the importance of Citrullus colocynthis L. Schrad as a promising biological and pesticidal agent useful for the treatment of various medicinal ailments as well as reducin...

Oluwatoyin Adenike Fabiyi

...cyst nematode Heterodera sacchari. Broadly, synthetic nematicides are utilized in the suppression of soil nematode infestation of sugarcane, with positive outcome in yield. However, emergence of resistant nematode strains and health hazards are associated with the ceaseless use of the synthetics. The efficacy of furfural (2-furanaldehyde) from agricultural biomass waste was examined as a practicable replacement to the regular synthetic nematicide. Agricultural...
Monif AlRashidi1*, Sami Saeed M. Hassan2 and Mohammed Shobrak3
...h) when the parent never sat on the egg during the period (02:00 - 03:59 h) and sat for only 16.67 % during the period (20:00 - 21:59 h). Moreover, the incubating adult seemed to avoid facing the sun which may be considered a behavioral mechanism to protect the egg from direct sunlight.
...
Misbah Sarwar1*, Abdul Hamid2 and Iftikhar Hussain2
...et, captive breeding and sale. The present study concludes that demoiselle cranes are being illegally hunted at a large scale in the study area resulting in decline in their population. There is a dire need of law enforcement to conserve the bird species in the study area.
...
Abdul Sattar*, Hasan Orooj and Muhammad Iqbal Afridi
...team of DHS (Health) envisaged that the load of disease followed by precise micro plan/ mapping that details out distribution of disease, vector (pre-adult and adult stage), as well as sectors vulnerable for dengue be earmarked for timely interventions in window period (winter). The results of high risk mapping followed by focused intervention are remarkable. The last 10 years data reflect the load of disease through these interventions introduced by DHS is at...
Abdul Sattar*, Hasan Orooj and Muhammad Iqbal Afridi
...team of DHS (Health) envisaged that the load of disease followed by precise micro plan/ mapping that details out distribution of disease, vector (pre-adult and adult stage), as well as sectors vulnerable for dengue be earmarked for timely interventions in window period (winter). The results of high risk mapping followed by focused intervention are remarkable. The last 10 years data reflect the load of disease through these interventions introduced by DHS is at...
Hailong Dong1, Fubin Gan2, Khalid Mehmood3, Jiang-Yong Zeng4, Rao Zahid Abbas5, Mushtaq Ahmad Gondal6, Zhenyu Chang1 and Qingxia Wu1*
... assay. The results showed that the 305/849 (35.92%) studied animals were sero-positive for F. hepatica with the further distribution of 24.20% (38/157), 38.02% (92/242) and 38.89% (175/450) from Bayi, Mainling, and Gongbo’gyamda areas, respectively. The seroprevalence of F. hepatica increased from 20.75% to 48.8% with age of yaks, with the further distribution of 20.75%, 30.23%, 39.55%, 37.70% and 48.8% for age g...
Nasir Ali Tauqir1, Asim Faraz2*, Annamaria Passantino3, Muhammad Asif Shahzad1, Rana Muhammad Bilal4, Adeel Tahir1, Hafiz Muhammad Ishaq2 and Abdul Waheed2
...considered as an indispensable source of animal protein and it is speculated that the demand of animal protein will be increased by 60-70% in 2050 for human consumption and much of this share will come from developing nations. This research was executed to find out the nutritional influence of corn steep liquor and enzose mixture as a non-conventional protein ingredient in day old broiler chicks. For this purpose, 42 days study was conducted using hubbard broi...
Shabana Naz1*, Nishat Ali Khan1, Arnab Tanweer1, Shifa Moazzam1, Qudrat Ullah2, Farkhanda Asad1, Irfan Khattak3, Sajida Batool4 and Rifat Ullah Khan5
... this purpose, the fecal samples were collected from 30 peafowl. Sampling was done thrice from same birds i.e., once before treatment and twice after treatment with Albendazole and Levamisole. The efficacy of Albendazole and the Levamisole against parasites was calculated. The fecal samples were examined by using flotation and modified Macmaster’s egg counting technique. The results...
He Yujiao1,2, Xie Huichun1, Lin Gonghua2,3, ZhangTongzuo2,3, Su Jianping2,3 and Du Yurong1*
... using mtDNA versus microsatellite markers. The most obvious difference between the two markers was reflected in the fact that the populations Yelashan (YLS) and Bangda (BD) represented a separate cluster based on the microsatellite data. Additionally, our studies involving the comparison of the two markers also reveal that a few of the differentiated populations from the middle region have a higher admixture. Due to a faste...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
...on and apoptosis of osteosarcoma cells through long non-coding RNA (lncRNA) LINC00857/microRNA (miR) -340. U2OS of osteosarcoma cells was treated with harringtonine at a concentration of 0.25, 0.5, 1, 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and colony ...

Nawab Khan1, Ram L. Ray2, Muhammad Ihtisham3*, Badar Naseem Siddiqui4*, Muhammad Khayyam5, Raheel Anjum6 and Simplice A. Asongu7

... northern Balochistan. A sample of 120 respondents was selected through multi-stage-random sampling from two tehsils (Sinjawi and Ziarat) of district Ziarat. Data were collected with the help of a pre-tested interview schedule. The association of some chosen socioeconomic characteristics like age, education, and size of landholding of the farmers with their awareness and adoption level was measured through the chi-squire (&c...

Rasool Bux Kalhoro1, Ghulam Mustafa Laghari2*, Ghulam Hyder Jamro2 and Muhammad Ibrahim Keerio2

...61.61 to 61.72 days to disappearance leghaemoglobium in the nodule.Furthermore, days to nodule formation and the number of nodules plant-1 were not significantly affected by seed rates, except row spacings. The 60cm row spacing had a positive relationship with nodules formation and their number in plant roots. In the case of crop growth rate (gm-2 day-1) and net assimilation rate (gm-2 day-1), both were significantly higher 0.719-0.707 and 17.46 to 17.12 gm-2 ...

Syeda Shazia Bokhari, Aisha Waheed Qurashi*, Roheela Yasmeen*, Fouzia Yasmeen, Nabeela Nayab, Uzma Rafi

...rds adherence and exopolysaccharides productions. Both productions are important parameters of plant growth promoting bacteria. Earthworms, along with soil sample, were collected from different areas of Lahore. Bacteria were isolated, purified and further characterized morphologically, biochemically and at molecular level. Bacteria after isolation were labelled as EPF1 while by molecular confirmation this bacterial strain wa...

Samreen Khan*, Salma Javed, Tabassum Ara Khanum, Nasira Kazi

...uary 2020. Total 73 soil samples retrieved from multiple fruits, vegetables, ornamental flowers, medicinal plants and other commercially important trees from different four sites of district Lakki Marwat and subsequently assessed the presence of EPNs using last instar larva of greater wax moth Galleria mellonella L. as baited host. After completion of requisite process, resultantly the corresponding recovery rate of EPNs was found 10.9% which includes only gen...
Funda Turan1*, Ihsan Akyurt2 and Sehriban Cek-Yalniz1
...over extract on sex reversal and gonadal development were also examined at the end of 120 days.At the end of experiment, highest feminization (89%) was observed at 50 mg L-1 red clover group. Morphological and histological examinations of the gonads in all groups revealed no intersex fish. Histological examination of fish treated with red clover extract revealed no damage to the testes or ovaries. Therefore, this study suggested that red clover extr...

Adel M. Abdelrahman1, Sahar R. Mohamed1, Soliman M. Soliman2, Sherif Marouf3* 

...tive Pseudomonas aeruginosa is harbor multidrug antimicrobial resistance of camel has serious consequences for human health, so, this study aimed to characterized of P. aeruginosa especially extended spectrum β-lactamases (ESBL) producing one; phenotypic and genotypic. The isolates of P. aeruginosa were confirmed biochemically by a Vitek 2 compact system (bioMe´rieux). Suspected...
Farah Deeba1, Hafiz Abdullah Shakir1, Muhammad Irfan2, Muhammad Khan1 and Javed Iqbal Qazi1*
...g medium (CPM) from soil samples collected from local termites’ influenced areas. Of all these, three isolates gave positive test for chitinase screened on the bases of clear zone on CPM following chitinase assay. The best chitinase producer was selected and identified as Bacillus subtilis employing 16S rRNA gene sequence identification technique. B. subtilis yielded highest chitinase on CPM at pH 7 with ...

Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4* 

...2018. For this, 264 milk samples and 155 swab samples were collected from 50 different dairy farms available in CMA and Patiya Upazila. A pretested questionnaire was implemented during sample collection for identifying the associated risk factors of udder lesions and SCM, and to identify the association of udder lesions with SCM. Clinical inspection, palpation and observation were the main...
Teuku Fadlon Haser1*, Muh. Saleh Nurdin2, Eddy Supriyono3, Deni Radona4, Fauziah Azmi1 , Kukuh Nirmala3, Widanarni3, Tri Heru Prihadi4, Tatag Budiardi3 and Riris Yuli Valentine5
...nserve mahseer in Atu Suasah and Lawe Melang Rivers, Aceh, Indonesia. This research aimed to analyse and compare of mahseer biology including sex ratio, length and weight relationship, Fulton and relative condition factor and fecundity to estimate the reproductive potential of mahseer in these two rivers. The study was conducted from July to December 2019. Mahseer samples were obtained from fishermen who captured the fish us...

Khlood G. Abdelkhalek*, Aly B. A. Badawy, Mohamed Fathi, Elshymaa A. Abdelnaby 

...mage analysis, and blood sampling were performed on a weekly basis. Results showed that FSH level was elevated (P<0.05) firstly from 5-months to 6.5-months-old, and then a second elevation was reported at 9.5-months-old, both E2 and NOMs levels were increased significantly (P<0.05) from 8-months to 10-months-old. Testosterone level showed a significant (P<0.05) increase from 8.5-months to 10-months-old, while LH level was elevated from 6.5-months to 7...

Mohammed Naji Ahmed Odhah1,2*, Dhary Alewy Almashhadany6, Abdullah Garallah Otaifah7, Bashiru Garba5, Najeeb Mohammed Salah2, Faez Firdaus Abdullah Jesse4*, Mohd Azam Khan G.K3 

...amar Province-Yemen. The samples were collected from the Central Corporation Slaughterhouse in Dhamar-Yemen from January 2019 to November 2020. During this period, a total of 29,071 and 5,705 samples were collected from local and imported food animals respectively. The results of analysed samples revealed that the distribution of the hydatid cysts among the local slaughtered animals were 3...

Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2* 

... governorate, Egypt. All samples were submitted for PCR test and other conventional methods of identification. Recovery of P. multocida isolates of diseased rabbits from lung, liver, and heart were 23 (46%), 11 (22%), 13 (26%), respectively, with a total incidence of 47 (31.3%). Biochemical reactions proved that 20 (42.5%) out of 47 isolates had the typical biochemical properties of P. multocida. However, only 10 isolates were identified as P. multocida using ...

Hailemariam Assefa1*, Tesfahun Dagnaw2 

....5% at 30oC while at the same operating condition ethyl acetate and methanol resulted a maximum yield of 17.0% and 16.5% and a minimum of 12.0% and 13.0% respectively. So petroleum ether is a good solvent to afford high yield in this work. The oil was analyzed by GC-MS and result in the identification of the compounds, (Palmitoleic acid methyl ester, Palmitic acid methyl ester, Linoleic acid methyl ester, Oleic acid methyl ester, Stearic acid methyl ester and ...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
...ithout leukoarais in the same period as the control group. ELISA was used to detect τ (Tau) protein expression level in the cerebrospinal fluid of the two groups. DTI technique was taken to compare DTI anisotropic diffusion coefficients of the two groups. According to our results there was a statistically significant difference in cerebrospinal fluid Tau protein concentration between t...
Syed Sikandar Habib1*, Saira Naz2, Sadia Nawaz1, Iqra Ameer1, Ameer Khatoon1, Hameed Ur Rehman3, Sahibzada Muhammad Jawad4 and Haider Ali5
...stan and worldwide. Fish sampling was done by using different nets (river and farm) and blood sampling was immediately drawn from caudal peduncle by syringe and added in EDTA containing vial and shake well for proper mixing. Vials stored in ice containing boxes and carried to laboratory for total complete blood count. The water parameter was analyzed in laboratory by taking the sample of w...
Nabila Rasheed1*, Bushra Waseem1, Kanwal Haneef2, Shumaila Usman3, Madeeha Sadiq1, Tabinda Urooj1, Sarah Tarique2 and Dabeeran Zeha4
...g potential by scratch assay method. Healing of the scratch was observed at different time intervals (0h, 6h, 24h, 48h). The images were analyzed by Image J software. Morphological analysis was done under inverted microscope. Results showed that with each passaging stage the viability of GDM cells remained normal whereas Wound healing potential of GDM-WJMSCs was decreased as compared to controls. Moreover, there were few mor...
Jhan Zeb1*, Saiqa Tufail1, Nausheen Saboohi1, Zafar Samuel2, Asher Azeem3, Yaseen Amir4 and Sehrish Akram5
...lpha;-linolenic acid, ecosapentanoic acid (EPA) and docosahexanoic acid (DHA). However, these fatty acids were found to be present in the flesh of L. rohita at the end of the experiment. The sum of saturated fatty acids (SFA) was higher as compared to the sum of monounsaturated fatty acid (MUFA) and polyunsatura...
Muhammad Anwarullah1, Aneela Zameer Durrani1, Muhammad Ijaz1Aftab Ahmad Anjum2, Muhammad Usman1*,Muhammad Zahid Iqbal1Muhammad Husnain1,Ahsan Anjum3, Qurratulain Ashraf4 and Ghulam Mustafa3

...r for MAP, so 1100 blood samples were collected from diarrheic animals or having history of some diarrheic episodes from four districts of Punjab i.e. Kasur (n=300), Rawalpindi (n=230), Lahore (n=230) and Bahawalpur (n=340). These samples were tested using ELISA and overall seroprevalence found was 39.64%. The major host-associated and non-associated ris...

Vladimir Safonov 

...evel in animals from the satisfactory region throughout all studied groups. Besides, an increased accumulation of lipid peroxidation products was observed. In particular, malonic dialdehyde increased by 17.9–96.6%. Also, the malfunction of the glutathione system was noted, manifested in the reduced activity of glutathione peroxidase and glutathione reductase. The consumption of Vitamin E as a non-enzymatic element within the antioxidant defense system in...

Sadarman1, Agung Irawan2, Muhammad Ridla3, Anuraga Jayanegara3*, Nahrowi3, Roni Ridwan4, Ahmad Sofyan5, Hendra Herdian5, I Nyoman Guna Darma5, Teguh Wahyono6, Dewi Febrina1, Rakhmad Perkasa Harahap7, Rizki Amalia Nurfitriani8, Danung Nur Adli9 

...t are widely proposed as safe additives to improve protein utilization in ruminants. This study aimed to investigate the effect of tannins extracted from acacia and chestnut on the degradation kinetics of ensiled and non-ensiled soy sauce residue (SSR) in sacco. Two fistulated beef cattle (425±25 kg) fed a diet consisting of 80% Pennisetum purpureum and 20% concentrate was used to i...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...quo;s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparativel...

Tilahun Woretaw1, Netsanet Beyero2*, Yonatan Kassu3 

... schimperiana. Purposive sampling technique was used to select two agroecology (midland and highland) and five representatives (two from midland and three from highland) kebeles based on the coverage of P. schimperiana. A total of 100 respondents, 58 from three high land peasant associations and 42 from two midland peasant associations, were purposely selected for an interview who had at l...

Muhammad Waryam Warraich1, Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2

...crop was harvested, soil sampling was carried out followed by analysis in the laboratory. It was observed that use of press mud @ Yield 1 % in integration with SOP as chemical K fertilizer performed as best treatment by improving yield (4.11 t/ha) and yield contributing parameters like maximum plant’s height (95.10 cm), length of spike (11.04), number of fertile tillers (5.67), 1000 grains weight (39.26 g) and grain count per spike (51).

...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...lants resulting in the disappearance of its wild populations in different mountainous regions in Pakistan. A proficient way for large-scale and fast propagation of C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and ...
Hongbin Li1,2, Lei Fang1, 3 and Lei Cao1,2*
... been applied as a universal explanation for spring migration timing of avian herbivores. However, studies of the green wave hypothesis have focused on species feeding intensively on above-ground primary production. To test whether a facultative grazer strictly follows the green wave, we used 16 complete tracks in time and space of spring migrating tundra bean geese (Anser serrirostris)fitted with telemetry devices from two flyways between Yangtze River...

Muhammad Zeeshan Majeed1*, Muhammad Irfan Ullah1, Dilbar Hussain2, Muhammad Luqman3, Muhammad Qasim1, Gulfam Yousaf1, Hamza Latif1 and Muhammad Zeeshan1

...nd filter paper-dip bioassay methods were used against sap-feeding pests and termites, respectively. Factorial analyses of variance revealed a significant impact of tested insecticidal formulations and exposure time on the mortality all insect pest individuals. The most effective differential chemistry insecticides against mango leafhopper, citrus psyllids, mango mealybugs and subterranean termites were nitenpyram, clothiani...

Sanjeela Sabahat1*, Juliya Abbasi2, Mushtaq Ahmad1, Saima Mumtaz1, Taj Naseeb Khan1, Sudheer Tariq1 and Muhammad Imran1

...awberry (Fragaria x ananasa) is a widely grown fruit crop known for its color, aroma and texture. The fruit is enriched with vitamin C, manganese and antioxidants. In Pakistan, except for a few low-temperature regions, it is mainly grown as an annual fruit crop where runners are transplanted every year. Owing to its short growing period and high profitability, it is mostly produced by small scale farmers. However, the changing climate from the past few years h...

Aftab Ahmad Sheikh1, Khalil Ahmed1*, Belqees Akhter1, Ghulam Qadir1, Muhammad Qaisar Nawaz1, Hafeezullah Rafa1, Abdul Wakeel1, Abdul Manan Saeed2

...ario, use of underground saline water for crop production with acceptable economic yield is a viable management strategy. Therefore, a lysimeter study was conducted to evaluate the effects of saline water on quality and quantity of forage sorghum (Sorghum bicolor L.). Forage sorghum was sown in cemented blocks and five increasing levels of saline water (0.30, 4, 6, 8 and 10 dS m-1) were us...

Stephen Chijioke Emencheta1, Chinelo Charity Eze1, Anthony Amaechi Attama2, Damian Ejike Agbo1 and Ebele Benedette Onuigbo1*

...riaxone (CRO)). Plaque assay was done to determine lytic action of the bacteriophage on the lawns of the Pseudomonas sp. The host range of the isolated bacteriophages was also assessed. Cultural characteristics and biochemical tests confirmed the Pseudomonas aeruginosa isolates. The bacterial isolates were sensitive to only ciprofloxacin. The Multi Antibiotic Resistance Indexes (MARI) of the isolated strains were greater tha...

Daniel Stewart Robertson

...ions supporting the proposals are presented.

...

Pham Tan Nha*, Le Thu Thuy 

...nd shrimp soluble hydrolysate with 1%, 2%, 3% and 4%, respectively (DM). The treatments were: SH0, SH1, SH2, SH3 and SH4, respectively. The results shown that daily weight gain (DWG) was lower for the ducks without supplementing shrimp hydrolyte (SH0 treatment) than those mix shrimp hydrolyte (drinking water) and the significantly higher result found in the SH2, SH3 and SH4 treaments (P<0.05). FCR of Ha Lan ducks were better in the SH3 and SH4 treatments (P...

Vahid Mollasadeghi1,2* and Samaneh Elyasi1,2

...ero), 1, 2 and 3 per thousand. PVY-activated tobacco leaf extract was used to infect the potato plant. This paper measured chlorophyll a, b, leaf area, number of leaves, stem length and tuber weight. The results showed that the symptoms of PVY were completely distinguishable on the leaves of tobacco plants compared to PVY on the leaves of Agria. PVY-infected leaves were more curled and paler than healthy leaves of cultivars Agria. The results of analysis of va...

Wassan Mhammed Husain1*, Zainab Sattar Ali2 

...of the experiment, blood samples were taken by heart puncture for serum collection. In comparison to all other groups, the current results reveal a significant (p≤0. 05) decrease in testosterone, somatic testicular index, and sperm quality in the G3 group, nevertheless, there was a significant (P<0.05) increase in LH, FSH, sperm abnormalities percent, and dead sperm percent in the G3 group. The capacity of Phitofert ® to restore all passive effects o...

M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2* 

...atory manifestation as nasal discharge, fever, off food, with ocular discharge and air gasping, paralysis of lips and salivation were subjected to clinical examination and a total of 141 samples were collected as follow; 111 nasopharyngeal swabs, 15 tracheal swabs and 15 lung specimens collected from camels from different governorates (New valley, Giza, Naj Hammadi (Qena governorate), Hala...

Noha M. El-Shabrawy1, Atef M. Kamel2, Aza S. Goda1, Gehad R. Donia1, Ahmed M. Salah-Eldein2* 

... feather. Also, 16 water samples were collected to assess the level of heavy metals pollution in the study area. The result revealed that the kidney is the most tissue burdens the highest concentration of heaviest metals among the examined tissues. Egyptian barn swallow and house sparrow have a great tendency to accumulate heavy metals in their tissues among other examined birds. Levels of Pb in Egyptian barn swallow and house sparrow have exceeded the normal ...

Arshad Farooq1, Abdul Hassan1, Muhammad Ishaq2, Asif Nawaz3*, Iltaf Ullah4 and Hidayatullah5

...out in two districts Charsadda and Nowshera of Khyber Pakhtunkhwa province during 2020 with main objectives to measure farmers’ knowledge level in fourteen climate smart recommended agricultural production technologies. The data were collected randomly from 60 sample respondents comprised of 30 respondents each from Charsadda and Nowshera districts through pre-tested interview schedu...

Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1

...verall, 390 no of sputum samples collected as of 800-participants comprised of TB patients (100), livestock(L/S) farm employees(200), abattoir employees(174), butchers(294), veterinarian(10) and veterinary assistants(22). Two out of 100 TB patients (2/100), 3 out of 200 livestock farm workers (3/200) and 3 out of 23 abattoir workers (3/23) were found positive for the presence of M bovis through PCR technique. A significant association (p<0.05) was found bet...
Samaria Nisar1, Tariq Manzoor Khan1, Muhammad Ahsan Iqbal1, Rahmat Ullah2, Muhammad Asim Bhutta3*, Saghir Ahmad3, Amna Bibi3, Hafeez ur Rehman4, Umar Farooq4 and Muhammad Zubair Ishaq5
...rsity of Agriculture, Faisalabad; Pakistan, during the normal growing season of cotton. A Randomized Complete Block design with three replications was used for the study. Plant height (PH), boll weight (BW), bolls/plant (BP), ginning out turn (GOT percent), hundred seed weight (100SW), yield/plant (YP), fiber fineness (FF), fiber length (FL), and fiber strength (FS) all revealed substantial variation in the analysis of variance (FS). The goal of this research ...
Ardi Prasetio1*, Christina Maria Sri Lestari2, Sutaryo Sutaryo2, Manar Fayiz Mousa Atoum3,4Muhammad Zahoor5, Asma Nisar6 and Muhannad Illayan Massadeh7
...abbit ration, has some disadvantages. Protein value in Black Soldier Fly Larvae -BSFL (Hermetia illucens Linnaeus, 1758) is equal to SBM and better in the amino acid profile. However, the chitin contained in BSLF may cause subclinical inflammation by blood parameter detection. Thus, this study investigated the BSLF substitution effect on rabbit blood traits. This study was performed using 28 wk old weaned male New Zealand White rabbits (1300 g ± ...

Faryal Malik, Mudassar Nawaz Khan* and Israr-ud-Din

...es in Siran revealed the same trend as observed in Atta Habib. Recovery capacity was least in Ghanemat-e-IBGE. The results suggest that the tested three wheat varieties suffered oxidative stress due to flooding but showed a recovery trend due to increased activities of antioxidant enzymes that scavenged the oxidative radicles, thus helping the wheat to recover. Atta Habib reflected higher recovering ability than Siran and Ghanimat-e-IBGE. The findings of the c...

Nguyen Binh Truong1,2,3*, Nguyen Van Thu3 

...tion and another one for sampling. The results showed that nutrient intakes (kg/animal/day) were similar (P>0.05) among treatments, however DM and OM intakes were numerically higher for the NDF55 treatment compared with the others. Digestibilities (%) of DM and OM were significantly different (P<0.05) among treatments and gradually decreased from the NDF47 to NDF59, however these values between NDF51 and NDF55 were not significantly different (P>0.05)...

Tamoor Ali1*, Muhammad Hamid Bashir1, Bilal Saeed Khan1, Abdul Ghaffar2, Danish Riaz3, Muhammad Zia ul Haq1, Shoaib Nawaz1, Aneeb Ali1, Iftikhar ul Hassan1, Ikram Ul Haq4, Muhammad Khuram Shahzad1, Usama Saleem5 and Tamsila Nazir2

...ment was conducted at Faisalabad in the trail region of UAF during 2018 to understand response of sucking insect pest fauna against the transgenic and non-transgenic cotton varieties. To figure out the response of these pest with the meteorological factors against whitefly, thrips and jassid. For this experiment RCBD Design was used. The five varieties of cotton were used in which IUB-2015, MNH-886, FH-142 and CEMB-33 were the Bt varieties while FH 942 was the...
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*

...f Pseudomonas aeruginosa which is responsible for the synthesis of long side-chain AHL (3OC12-HSL), and gene yenI of Yersinia enterocolitica which is responsible for the synthesis of short side-chain AHL (C6-HSL), were selected and transformed into ETEC strain C83902, respectively, and endowed with the ability of endogenous synthesis of 3OC12-HSL and C6-HSL. At the same time, 200 μmol/L 3OC12-HSL and ...

Altaf Hussain1*, Muhammad Saad Ashraf3, Asim Shamim1, Mohsin Nawaz1, Arslan Mehboob2 and Urooj Fatima2

... justify;">Infectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is one of the most devastating and immunosuppressive diseases of the poultry and has been a constraint on the sustainable food security around the globe including Pakistan. Poultry industry is the second biggest industry in Pakistan while IBD is an important disease seriously threatening poultry farming. Despite the use of mass and ...

Prima Mei Widiyanti1*, Mirnawati B Sudarwanto2, Etih Sudarnika2, Raphaella Widiastuti1 

...biotic in milk. The milk samples spiked with enrofloxacin antibiotic were submitted to thermal treatments. The milk samples were analyzed using high performance liquid chromatography (HPLC) method. This study showed that no heating (control), heating at 72 oC for 15 seconds (high temperature short time pasteurization), and 89 oC for 1 second (higher heat shorter time pasteurization) did not affect on enrofloxacin antibiotic ...

Abbas M. Al-Azab1*, Ahmed A. Zaituon2, Khalid M. Al-Ghamdi3, Fahd M. Abd Al Galil4,5 

Ijaz Rasool Noorka1,2*, Bilal Ahmad Khan3*, Kafeel Ahmad4, Zafar Iqbal Khan4, Muhammad Ather Nadeem3, Amer Nawaz2, Tasneem Ahmad5 and Humayun Bashir4

... were selected. Standard samples preparation methods were worker to check occurrence of the heavy rock’s metals in the forage and soil samples. The Atomic Absorption Spectrophotometer was used to check Iron (Fe). The results revealed that Fe were found in safe limit, as per WHO. 

...

Amal H. Mostafa, Mohammed Y. Matoock, Heba M. A. Khalil* 

...essful; in terms of time-saving, accuracy, and reliability in assessing and managing free roaming dog’s aggressive level.

Keywords | Free roaming dogs, Aggression, SAFER, MATCH-UP-II, Management 

...
Tabinda Urooj1,*, Bushra Wasim1, Shamim Mushtaq2, Syed Nudrat Nawaid Shah1, Lubna Faisal3, Moazzam Ali2, Nabeela Rasheed1 and Syed Faizan Ali Rizvi4
...istory of contraceptive usage. Also, most of the females have three children or more with the history of breast feeding. It was concluded that, lack of education combine with low socioeconomic status, which further aggravates the health outcomes especially by neglecting the early diagnosis of the disease. Also, higher risk was observed for married women, housewives with extended family that is 3 or more children. Breast feeding for extended duration (at least ...

Muhammad Iqbal Jakhro1*, Nadeem Sadiq1, Javed Ahmed Abro1, Amanullah1, Fateh Muhammad2, Maqbool Ahmed3, Syed Ishtiaq Ahmed Shah3 and Qasid Hussain4

...of Olive (Olea europaea) saplings as well as to determine the soil physiochemical properties. One-year old Olive plants were applied with organic and inorganic mineral fertilizer application for six months. The results showed that in comparison to control, plants fertilized with T2 (N 50g/plant), T3 (P 25g/plant), T4 (K 25g/plant), T5 (NPK 50:25:25/plant), T6 (Biochar 2:2) and T7 (FYM 2:2) had significant results. The studied parameters which was height of pla...

Muhammad Rizwan1*, Jehanzeb Farooq1, Amjad Farooq1, Muhammad Farooq1,2, Ghulam Sarwar1, Muhammad Nadeem1, Muhammad Riaz4, Muhammad Rafique Shahid3, Hafiz Ghazanfar Abbas1 and Muhammad Kashif Shahzad Sarwar1

...ton Research Station, Faisalabad. Highly significant results were noticed in the analysis of variance. Further investigation in correlation analysis revealed that there was comendable positive association among leaf chlorophyll contents, seed cotton yield (SCY), No. of sympodia, seed index and boll weight(g). Principal Component Analysis (PCA) demonstrated that only 02 out of 08 PCs showed eigen value greater than 1. The contribution of first two PCs in total ...

Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2 

... handlers working in the same places yielded 13 Salmonella isolates, at the time 4 isolates were only obtained from 50 hospitalized patients with diarrhea. From the public health point of view, S. typhimurium, S. enteritidis, and S. kentucky were serotyped from both food and human samples. Epidemiologically, insignificant sex risk factor was statistically found in this study although Salmonella was more common in males (14.6...

Anguara Khatun1*, Sachchidananda Das Chowdhury1, Bibek Chandra Roy2, S.M. Shafikul Gani3, Bipul Chandra Ray1, Tanvir Ahmed1 

...ing 20 birds each. The basal diets were corn-soya broiler starter and broiler grower diets. Starter diet was fed up to 21 days and grower diet during 22-35 days. In treatment 1, chicks were fed a basal diet (Diet 1), in treatment 2, chicks received a basal diet plus a multi-strain probiotics @100g/100kg feed (Diet 2) and in the third treatment, the basal...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
...hich included control (basal diet), control +0.25% AZO, control + 0.50% AZO and control + kitasamycin (as AGPs). The results indicate that LBGW, ADG and FCR was significantly (P<0.05) improved in AZO 0.25% and 0.50% than the control. Eviscerated (EV), breast muscle (BM) and leg muscle (LM) were significantly (P<0.05) higher in the AZO 0.50% treated groups than control. Digestibility of DM, CP, AME, Ca and P digestibili...

Mohammed Sirajul Islam1,2*, Nurhusien Yimer1*, Wahid Haron1 , Faez Firdaus Abdullah Jesse1, Mark Hiew Wen Han1, Mamat-Hamidi Kamalludin3, Wan-Nor Fitri1, Ubedullah Kaka4, Abdul Quddus1,5 

...ages were kept under the same paddock and fed with equal quality and quantity Guinea grasses and palm kernel cakes. Body weight was measured at monthly basis by using a digital animal weighing scale. Age at sexual maturity was determined by the time of semen ejaculation and quality. The results revealed significantly (p<0.05) greater body weight in KK × Brangus bulls (230.50±9.3 k...

Fang Zhao, Gen Wang, Xiaobin Li, Guodong Zhao, Hui Chen, Chen Ma and Kailun Yang*

...(CT, corn-soybean meal basal diet), CT + 111 (CT + 111 mg/kg BW RPT 5-HTP), and CT + 222 (CT + 222 mg/kg BW RPT 5-HTP) groups. The experiment lasted for 16 d. On the 16th day of the experiment, blood samples from the sheep were collected at 0 h before feeding and at 1.5, 3, 4.5, 6, 8, 10, and 12 h after feeding in the morning. The plasma concentration of 5-HTP, tryptophan (Trp), 5-hydroxytryptamine (5-HT), and MLT was determ...

Mustafa Ozan Atasoy

...urred the development of safer and efficient vector vaccines, which convey immunogenic genes of interest obtained from vitally important diseases. Herpesvirus of turkeys (HVT) has long been considered a versatile tool owing to its non-pathogenic characteristic, relatively higher transgenic capacity, and enabling the DIVA strategy. Numerous bivalent and multivalent HVT constructs have been generated by various methods and som...

Shireen S. Aboelwafa¹, Alsagher O. Ali², Rania Hamada³, Hassan Y.A.H. Mahmoud2* 

...e-linked immunosorbent assay (ELISA) to assess the seroprevalence in 92 sheep and 92 goats from four districts in Luxor governorate (Al-Qurnah, Armant, At-Tod, Esna). A total of 92 sheep were sampled, of which 37 (40.2%) were positive for T. gondii, six (6.5%) were positive for N. caninum and four (4.3%) were positive for both. Out of 92 tested goats 32 ...

Kusuma Adhianto*, Satria Ibnu Lenanto, Akhmad Dakhlan, Muhamad Dima Iqbal Hamdani 

...vey method, the research sample was determined by purposive sampling. The data were analyzed using the paternal half-sib correlation method. The observed variables included weaning weight and yearling weight. The results of this study showed that the average weaning weight of female Saburai goats in Sumberejo District was 16.4±2.0 kg, and the average weight for one year was 36.9±2.5 kg. The weaning weight and y...

Hongqun Li1,*, Liganag Xing1, Xiaoli Liu2, Yonglan Pu3, Yuqing Yang3 and Yongyao Fu1

...at Maxent model provided satisfactory result with the AUC value of 0.871 and 0.841 for the model training and testing, respectively. The most significant factors were maximum temperature of hottest month, mean temperature of hottest quarter, altitude, annual mean temperature and minimum temperature of coldest month, with the thresholds 30.5~31.8°C, 27.5~28.7°C, ≤ 400 m, ≥ 17.5°C, and ≥ 2.3°C, respectively. Under the current conditions ...
Elfadil Elfadul Babiker1, Khalid Ahmed Abdoun2*, Fahad AL Juhaimi1, Kashif Ghafoor1, Riyadh Salih Aljumaah2 and Ahmed Abrahim Al-Haidary2
... MPD) for 6 weeks. Blood samples were collected every 2 weeks throughout the experimental period. Vitamin C concentration and the total antioxidant capacity of serum increased by > 33% in animals fed on Moringa-supplemented diets. Dietary supplementation with Moringa enhanced serum catalase activity by > 9% as a function of feeding period. Animals fed on Moringa-supplemented diets also showed reduced serum concentration of malondialdehyde (MDA) by > 4...

Tintin Rostini1*, Irwan Zakir1, Danang Biyatmoko2 

...nts consisting of TR0 (basal ration of palm waste-based local feed without Curcuma and yeast), TR1 (basal diet with 0.5% yeast), TR2 (basal diet with 2% Curcuma flour) and TR3 (basal diet with 0.5% yeast and 2% Curcuma flour). Meanwhile, the variables measured include consumption, dry matter digestibility, organic matter, crude protein, NDF, ADF, daily b...

Mary A. Sargious1, Hanaa A. Ahmed1, Ragab M. El-Shawarby2, Hatem H. Bakery2, Sherif I. Ramadan3, E. Gus Cothran4 and Ayman Samir Farid5*

...f 229 Arabian horse hair samples including 120 samples from El-Zahraa stud and 89 from two private farms in addition to 20 hair samples of Dutch Warmblood were genotyped by 16 microsatellite markers. The purposes of this study were; firstly, to investigate the current status of the genetic diversity and inbreeding of Arabian horse populations reared in E...
Lin Ye, Jie-Lan Jiang*, Jia Xu, Rui-Ting Liu, Yin-Qiu Tian, Qing-Qing Zang, Jia-Yi Cao and Ming-Guang Mao*
... role of CLCN5 under low-salinity stress, a cDNA sequence of CLCN5 (TrCLCN5) was cloned from Takifugu rubripes using the reverse transcription PCR (RT-PCR) technique. The results showed that TrCLCN5 cDNA contained a complete open reading frame of 2505 bp continuously encoding 834 amino acid residues. Sequence alignments revealed that the TrCLCN5 cDNA is highly homologous (96.2% and 96.1%) with that of Takifugu bimaculatus and Takifugu flavidus. SignalP and TMH...
Imran Bodlah1*, Muhammad Tariq Rasheed1, Muhammad Farooq Nasir1, Tariq Mahmood2 and Muhammad Asif3
...(Loew, 1858), Allobaccha sapphirina (Wiedemann, 1830), two species of Coccinellid beetles, Coccinella septempunctata (Coleoptera: Coccinellidae), Menochilus sexmaculatus (Fabricius, 1781) and Chrysoperla carnea (Stephens, 1836) (Chrysopidae: Neuroptera) were found to be the bio-control agents of P. simlae.

...

Mohammad Hussain Haidary1, Rozanaliza Radzi1*, Muhammad Waseem Aslam1, Seng Fong Lau1, Farina Mustaffa Kamal2, Ahmad Rasul Radzali1 

...lateral to the palatoglossal folds. Descriptive statistics were derived from the retrospective data using simple percentages. Fifty-seven cats were analyzed up to the final treatment with known survival status. The majority affected cats age between 3-6 years old (24/57) and male (41/57). Most affected cats came from indoor multicats households. The two most common clinical signs were halitosis (42%) and anorexia (28%). Caudal stomatitis (100%) and gingivitis ...

Muhammad Khubaib Hameed, Muhammad Aziz ur Rahman, Muhammad Ashraf, Safdar Hassan, Muhammad Riaz, Muhammad Qamar Bilal, Fawwad Ahmad, Muhammad Sharif*

...immune organs such as bursa, spleen and thymus weight were also recorded. Weekly feed intake (3506g, 3531g) and body weight (2080g, 2139g) were better (P<0.05) in group fed arginine supplementation as 0.4 and 0.6%, respectively as compared to control group. Better feed conversion ratio (FCR, P<0.05) was shown by the all arginine treated groups with best FCR result in D group. Birds fed diet containing higher level of arginine (0.6%) had higher (P<0.05...

Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3

.... quinquefasciatus. Bioassay of whole-plant extracts was performed following WHO methods, with slight modifications. LC50 values for A. scoparia and A. indica against early fourth instar larvae were 360.4 and 971.1 ppm, respectively. LC50 values for pupae were 1665 and 2838 ppm for A. scoparia and A. indica extracts, respectively. Percent knockdown after 1 h exposure was 49.0 for A. scoparia. KDT50 and KDT90 values for A. scoparia were 69.7 and 763.5 min, resp...

Muneeb M. Musthafa1,* and Fauziah Abdullah1,2,3

...r’s hill, Gunung Besar Hantu and Gunung Angsi at lower altitudinal (500 m) cline was selected for sampling, where light, malaise and pitfall traps were utilized during 2013-2014 season. Altogether from these three sampling sites 1,575 beetle samples were collected and they went through with some diversity analysis. The Margalef index for Gunung Be<...

Khadim Hussain Memon1,*, Amir Mahmood Memon2, Tahira Jabeen Ursani3, Abdul Manan Shaikh1 and Sapna Waheed Memon1

I Nyoman Sulabda1*, I Nengah Wandia2, I Ketut Puja3 

...BoLA gene, the BoLA microsatellite loci (DRB3 and BM1815) were used to evaluate allel diversity using 18 samples of Taro white cattle. Genetic variability was low, with observed heterozygosity and expected heterozygosity for locus BM1815 was 0.778 and 0.51, and for DRB3 was 0.556 and 0.478, respectively. Moreover, the polymorphic information content (PIC) for these two microsatellite was ...

Sadaf Shahid1, Abdul Razzaq2, Gul-Makai1, Asim Shamim3*, Hafiz Muhammad Rizwan4, Rana Hamid Ali Nisar5, Qaiser Akram6, Mohsin Nawaz3 

... Ixodes (3.98%), Haemaphysalis (1.90%), Hyalomma (7.38%), Rhipicephalus (7.14%), and Boophilus (2.62%) were prevalent in the study area. A non-significant association (P > 0.05) of tick infestation with sheep and goat breeds was found in the present study. The highest prevalence of ticks was found in animals of 1-2 years of age (15.79%), followed in order by less than one year (11.44%), and 2-3 years (9.06%) of age. Females (15.56%) were found more prone to...

Jiao Ma*

...lecular processes and messaging pathways involved in success Cardiac stem cells offer an effective approach to the production and presentation of cardiac lesion therapy using cardiac stem cells.

...

Irshad Ahmad1,2*

...ment through pheromones, sanitation of the crop and field, removal of the infested frond and offshoot, eradicating the concealed breeding sites of the pest. IPM also include cultural techniques comprising in-groove humidity and palm density, judicious use of insecticides, using biological control agents including entomopathogenic fungi, nematodes as well as adopting the regulatory methods of domestic and international quarantine. This paper provides updated in...

Amjad Ali Channa1, Mansoor Tariq1*, Zaheer Ahmed Nizamani1, Nazeer Hussain Kalhoro2 

...akistan. A total of 1920 samples, including each of 960 blood samples and tracheal swabs were collected from broilers and tested through hemagglutination inhibition (HI), AIV-ELISA and RT-PCR. The HI test revealed a significant (P<0.01) difference in the seroprevalence of H9 virus (40.11%), H5 virus (16.88%) and H7 virus (6.25%) respectively. The mean...

Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1 

...types of NDV. Out of 550 samples were collected during an active surveillance in 2020 and 2021 from different poultry farms in five Egyptian governorates, 100 pooled samples were tested for NDV identification. NDV replication and growth was detected by embryo mortality after inoculation of all samples in one passage in embryonated chicken eggs and furthe...

Ziyue Qin1, Ali Mujtaba Shah2, 3, Qing Zhu1, Yan Wang1, Diyan Li1, Gang Shu4, Yaofu Tian1 and Xiaoling Zhao1*

...ivalent volume of normal saline. In the IST test, twelve chickens on d 70 fasted 12 h from each stock were assigned into four groups, respectively. Each of 3 birds was injected with human insulin at the dosage of 40, 60, and 80 µg/ kg BW via intraperitoneal injection, respectively. In both trials, the blood glucose concentration was determined at 0, 10, 20, 40, 60, 120, and 180 min through small brachial blood vessels....

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...atitis and chronic compensatory alkalosis. The X-ray examination revealed pleural effusion of the dog; B-ultrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphom...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...atitis and chronic compensatory alkalosis. The X-ray examination revealed pleural effusion of the dog; B-ultrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphom...

Guangming Xiang1, Ran Di1, Yaowu Wang1, Shangquan Gan2, Shouren Liu2, Xiangyu Wang1, Wenping Hu1, Qiuyue Liu1* and Mingxing Chu1*

Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
... (NDV) and infectious bursal disease virus (IBDV) were not affected by enzymatic inductions. Therefore, the antigen prepared by phospholipase C has been found to be more effective for HI test for rapid diagnosis of IBV during infection.

...

Sherif Abdelghany1, Lynda Allouche2, Ahmed A. Abd El-Maksoud3, Ehab N. Daoud4, Saleh A. Kandeal1, Mohamed A. Radwan1* 

...son, which supports milk safety and dairy chain sustainability. Forty-two Holstein cows in the middle of the first lactation were used and divided into a control group (22 cows) that received control diet and a treatment group (20 cows) that received a control diet supplemented with 200 g of potassium carbonate through 30 days. Milk yield for all cows were recorded, while a total number of 84 milk samples were collected...

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

...e-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the diagnosis of infection with H. Pylori in human stool and fasces of dogs and cats. Two hundred stool samples from humans and eighty-eight fecal samples from dogs and cats were collected with gastric disorders. The presence of H. Pylori infec...

Saeed Ahmed Essote1, Asim Iqbal1, Muhammad Kamran Taj2*, Asmatullah Kakar1, Imran Taj2, Shahab-ud-Din Kakar1 and Imran Ali 3,4*

...palerosophis (6.3%) and Psammophis. The Viperidae family has been on the second in occurrence with four genera viz., Echis (4.5%), Eristocophis (2.4%), Psudocerastes (2.9%) and Vipera (3.5%). The Typhlopidae (Ramphotyplops 5.8%, Typlops 4.6%) and Elapidae (Bungarus 6.3%, Naja 5.3%) families were represented with two genera each. While Boidae (Eryx 4.48%) and Leptotyphlopidae (Leptotyplos 7.5%) families had only one genus each. The highest diversity was recorde...

Mohamed S. Hussein1*, Hanan A. Fahmy2, Ashraf A. Abd El Tawab3 

...ed that 92% (138/150) of samples were found positive for E. coli. The antimicrobial susceptibility testing of 72 confirmed E. coli isolates against seven quinolones and fluoroquinolones antibiotics revealed that 62 (86.1%) were resistant to at least one antibiotic, with highest resistance rates observed against first generation (86.1% for nalidixic acid, and 81.9% for flumequine), and lowest rate against levofloxacin (33.3%). A total of 24 isolates, dsplaying ...

Uzma Ishaq1, Shahnaz Dawar2, Nasira Kazi1, Erum Iqbal1* and Saboohi Raza3

...cro;m long, post uterine sac 10-16 µm in length, apex of dorsal tooth 3-5 µm from anterior end. Morphometric and morphological data of O. paraobtusus Jairajpuri and Khan, 1982 and O. obtusus Cobb, 1913 are also given. These species are described and redescribed for the first time from Pakistan.

...

Tran Thi Bich Ngoc1,2, Ninh Thi Huyen1,2, Nguyen Cong Oanh2, Pham Kim Dang2* 

...l diets consisted of a basal diet (CONT), PROB diet (CONT + 0.2% PROB), ORAC diet (CONT + 0.2% ORAC), and PROR diet (CONT + 0.2% PROB + 0.2% ORAC) for 60 days including 2 periods (25-45 kg and 45-65 kg). In comparison to the CONT group, dietary supplementation of PROB or ORAC or PROR increased ADG and decreased FCR (P < 0.0001), with the higher ADG and lower FCR for PROR group during the entire experimental period. Pigs fed PROB or ORAC or PROR diets led to...

Esraa Yosry Abdel Halim1, Hamdy El-Essawy1, Abeer Abdel Nasser Awad1, Mona S. El-Kutry2, Lamiaa Ibrahim Ahmed1* 

...e dairy products’ usage, recent growth of globalization and the widespread of international trade mandate the microbial evaluation of the imported dairy products. In the present study, ninety random samples of imported milk powder, labneh and cheddar cheese from New Zealand, Ireland, Turkey, Poland, Netherlands, European Union and Switzerland were collected from the Egyptian markets then were investigated organoleptica...

Pham Tan Nha1*, Nguyen Thi Kim Dong2, Le Thu Thuy1 

...3 and 0.4 % (in DM) to basal diet. Corresponding to the G0, G0.1, G0.2, G0.3 and G0.4 treatments. The results showed that the daily intakes of DM, OM, CP and EE were signiificantly higher (P<0.05) for the 3 last treatments (the G0.2, G0.3 and G0.4 treatments). The significantly higher daily weight gain, final live weight and the lower FCR were found for the G0.3 treatments (P<0.05). The ginger supplement in the diets of Tau Vang chicken improved the carc...

Masouras P.K.1, Nikolaou K.1,2, Laliotis G.P.1*, Koutsouli P.1, Bizelis I.1 

...ality characteristics on samples obtained from cattle reared for meat purposes in Greece and to investigate if any correlation between these characteristics, IMF and marbling exists. Thirty steak cuts (12th thoracic rib) obtained from carcasses of both sexes of three different group of breeds (Holstein, Greek Red breed, crossbreds) were used to determine color parameters (L*, a*, b*), drip and cooking loss, tenderness and IMF. Marbling was estimated using an i...
Shehzadi Saima1*, Faiza Ghaffar1, Ghulam Yasin1, Muhammad Nawaz2 and Khalid Masood Ahmad1
... germinations under high salt concentration is very vital. With this object we observed the effects of various levels of NaCl (0, 25, 50 and 75 mM) on six varieties (Green leaf, Rama posa, Arka anamika, Super green, Okra kashish and Nerali) of Okra. The results clearly indicated that seeds of all varieties can tolerate the lower concentration of salt (25 mM) and higher (50 mM) greatly redu...

Abdul Haleem1*, Ghulam Hassan1, Arshad Iqbal2, Fahim Ullah Khan3, Muhammad Sajid3, Farhad Ahmad4, Rafi Ullah Khan5 and Mohammad Ilyas2

...es, parents, F2 progeniessand parent versus F2 progenies for all the studied traits. Among parents, best lines were Atta-Habib for plant height (95.10cm) and tillers plant-1 (12.30), Janbaz for economic yield per plant (20.93g) and spike length (10.87cm), Khatakwal for 100-grain weight (3.27g). Among F2 progenies, best combinations were Khatakwal × Lalma-13 for plant height (93.96 cm), Janbaz × Pirsabak-05 for ti...
Seble Mulugeta1 and Amenu Leta2*
...-stage stratified random sampling was employed to draw 210 respondents. The descriptive statistics and multinomial logit and the binary logit model were used to analysis the data . The descriptive result revealed that a total of samples 37 (17.6%) were adopter of modern energy sources, 50(23.8%) were adopter of both traditional and renewable energy technology, and 123 (58.6%) were user of traditional energy source. The binar...

Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2 

...ering from infectious bursal disease (IBD) for isolation of fungal species on specific medium. A total of 19 purified fungal isolates have been identified morphologically. Aspergillus isolates were the most identified (42%), followed by Trichosporon (10.5%), Penicillium (10.5%), Fusarium (5%), Candida (1%) and non-identified isolates (26%). Furthermore, the obtained 8 Aspergillus isolates were further morphologically identif...

Mahreen Alam*, Muhammad Ashfaq, Sarfraz Hassan and Asghar Ali 

...iry animals. Groundwater samples were analyzed for the Total Dissolved Solid, Electric Conductivity, and potential Hydrogen. The groundwater quality was good at the tail, marginal at the middle, and poor at the head reaches of the distributary. Primary data were collected from 300 respondents along 11-L distributary located in District Sahiwal, Punjab-Pakistan during 2018-19. A stratified random sampling technique was used t...

Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6 

...ved by using the TBARS Assay Kit, also the superoxide dismutase activities. As supporting data the histological analysis of the mother’s and offspring’s liver and spleen were prepared by using the paraffin method. There were sigificant results in the parasites levels and the increase in P. berghei infection followed an increase of oxidative stress in mothers and offspring mouse in the treatment and control groups. The liver and spleen of mothers ha...

Fulencio Ferretiz-Rodríguez1, Jose Alejandro Roque-Jiménez1, Héctor A. Lee-Rangel1*, Gregorio Alvarez-Fuentes1, Juan Carlos Garcia-Lopez1, Rolando Rojo-Rubio2 

...1 ± 0.16) fed a basal diet, were randomly assigned to one of four treatments: Control diet, Biocholine (BIO) supplementation (15 grams/cow/day), Optimethionine (OP) supplementation (9 grams/cow/day), and BIO x OP supplementation, with a duration of 60 days. Optimethionine treatments decrease milk production (P ≤ 0.05), but fat corrected production was higher (P ≤ 0.05) when cows were supplemented with BIO. The chemical composition of the milk did ...

Jarmuji Jarmuji1,3, Lili Warly2*, Mardiati Zain2, Khasrad Khasrad2

...d to obtain the level of sakura block plus as an in-vitro supplementary feed in Oil Palm Fronds (OPF) feed on optimizing rumen fermentation products, digestibility of nutrients, and gases production. Sakura block plus is a modification of commercial sakura block by using earthworm flour as a source of protein as a substitute for rice bran. The treatments were arranged as follow: P0= OPF + concentrate + 10% commercial
Indah Prihartini1*, Miftachi Ari2, Manar Fayiz Mousa Atoum3,4, Akhis Soleh Ismail1 and  Listiari Hendraningsih1
... in rice straw (Oryza sativa L.) on digestibility and efficiency of microbial protein synthesis using in vitro residual gas production and the optimal lignolitic probiotic in rice straw. The materials used were rice straw IR 64 cultivar and lignolitic TPG probiotic. The method used was Randomized Complete Block Design (RCBD) with four levels of treatments and three groups. In addition, a further test was conducted using Duncan’s multiple range due...

Martha Echioda Ogbole*, James Agbo Ameh, Samuel Mailafia, Olatunde Hamza Olabode and Bridget Jessica Adah

...text-align: justify;">Lassa fever (LF), caused by Lassa virus (LV), is a severe and acute viral haemorrhagic fever. The LV is a single-stranded RNA virus of the family Arenaviridae, transmitted by the Mastomys natalensis rodent. This retrospective study was undertaken to determine the annual prevalence, distribution and case fatality rate (CFR) of LF in Nigeria with a view to establish the disease burden and dynamics, and to...

Shang Zhenda1,2*, Kong Qinghui1,4*, Li Jiakui1,4, Liu Suozhu1,2, Tan Zhankun1,2, Shang Peng1,3 and Wang Honghui1,2

Oce Astuti1, La Sara2*, Muzuni3 and Safilu4

...it were studied. Monthly samples from different habitat characteristics of intertidal zone (station A), river mouth (station B), sea grass (station C), and water depth of > 30 m (station D) using collapsible crab pots and gillnets were recorded, identified its species, sexed, counted its number, and analyzed its spatial and temporal CC and SR. The Chi-square test (α = 0.05) was used to test significant differences of expected 1: 1 SR. It was 12 DC spe...

Yao Zou, Shien Ren, Miao Xu, Nannan Liang, Xuxin Zhang, Chongxuan Han and Xiaoning Nan

...uch as cranial length, basal length and cranial base could be key measurements for cluster and classification. Zygomatic breadth could be considered as quantifiable differences between the two genera Eospalax and Myospalax. We suggest that the clustering patterns could be related to climate and geographical factors. Finally, it was found that most of the skull measurements were positively correlated with latitude but negatively correlated with annual mean temp...

Yongjie Xue1, Jinling Yan1, Huifeng Zhao1*, Haijing Zheng2 and Changhai Ma1

...uld be considered in the same manner as the issue of national security. This study explores how China’s beef cattle industry will react to the international impact of soybean and beef trade. In contrast to previous studies, we put beef and soybean imports into the same model and studied them simultaneously, which is more in line with actual industrial development. Based on the Error Correction Model (ECM) and Vector Au...

Abdul Jabbar*, Anees-Ul-Hussnain Shah, Abdul Basit, Ghulam Ahmad, Aftab Ahmad Khan, Suleman Raza, Muhammad Sultan Ali Bazmi, Imtiaz Akram Khan Niazi and Ahmad Hussain

Lanjie Li1, Jingjing Zhang2, Ruiyan Zhang2, Ning Zhang2, Zixiang Wei2, Guiqin Liu1,*, Riaz Hussain Pasha3, Muhammad Akram Khan4 and Saif-Ur-Rehman5

... oligopeptides in hydrolysates were studied. The optimum enzymatic hydrolysis conditions were identified at 5.96 pH, 0.7% enzyme-substrate ratio, 55ºC temperature and 3 h time. Under these optimum conditions, DH of 25.4%, PR of 95% was obtained. Moreover, hydrolysates were rich in oligopeptides, especially di- and tri-peptides and demonstrated good antioxidant activities. These results introduce a new methodology with t...
Furqan Awan1,2, Sadia Nawaz3, Muhammad Muddassir Ali3,*, Asia Iqbal4, Raheela Akhtar5, Sehrish Firyal3, Haleema Sadia6, Arshad Javid4, Muhammad Rashid7, Muhammad Sulman Ali Taseer8 and Zhenling Zeng1
...actices were convenience-sampled on the basis of voluntary participation. Forty practices were approached, and veterinarians from 24 practices agreed to participate. Data were provided on 96 original presentations, excluding revisits, of which 47.92% were intact males and 52.08% were intact females with the mean age of 3.98 years. Moreover, further 96 controls were selected from the same clinical practices with

Juliet N. Ozioko1, Obinna V. Ayogu2, Benjamin O. Ezema1, Dilibe C. Uramah3 and Kingsley O. Omeje*,1

...ds, flavonoids, tannins, saponnins and terpernoids were detected. The leaf extract had 0.415 % of alkaloids, 0.102 mg/TAEq of tannins, 0.211 mg/QEq of flavonoids and 0.360 mg/GAEq of phenolics. Phytol, oleic acids, hexadecanoic acids and octadecanoic acids were detected in the leaf extract by Gas Chomatography-Mass Spectroscopy. White blood cells, hemoglobin and platelets were some of the hematological indices increased after the oral administration of the ext...
Muhammad Qasim Mazari1, Dildar Hussain Kalhoro1,*, Hasina Baloch1, Muhammad Saleem Kalhoro2, Shahid Hussain Abro1, Rehana Buriro1, Asmatullah Kaka1, Fahmida Parveen1, Mazhar Hussain Mangi3, Ghulam Murtaza Lochi1, Abdul Ahad Soomro4, Ali Gul Soomro1, Abdul Ghaffar Abbasi1 and Sarfraz Hussain Depar1
...ent study a total of 800 samples were collected from two hundred descriptive (Holstein Friesian) and non-descriptive (Thari) cattle breeds from districts of Mirpurkhas and Badin, 400 samples were taken from each district. Animals were first screened through Single Intradermal Tuberculin Test (SITT) then positive and negative reactors of SITT were further investigated through Rapid BTB Ab test, culture examination and ELI

Shuangye Wang1,2, Yunlin Zhao1,2, Zhenggang Xu2,3,*, Junzhi Chen3, Guiyan Yang3, Song Wang2 and Kangkang Jiang2

 

...ger than in 2016 for the same period; (2) The milu population lived together in the released area. They even moved to outside of Dongting Lake in summer and moved back in autumn with the water level change. The milu population separated into several groups during the movement process; (3) By combining home range and the maximum flooded time index map, the milu population habitat covered various types of land use and active area when water source was included. ...
Dyah Roeswitawati1*, Iva Kristova1, Muhidin Muhidin1, Otto Endarto2, Manar Fayiz Mousa Atoum3,4, Irum Iqrar5,6 and Luqman Ali Shah7
 
...paya L.) leaves, chrysanth(Chrysanthemum morefolium Ramat.) leaves on the persistence of red mites. This research was conducted at the Research Institute for Citrus and Tropical Fruit Plants in Batu City, East Java, Indonesia from July to September 2017. The study was a factorial experiment, the 1st factor is the type of natural pesticides: A1 = soursop leaves, A2 = papaya leaves, A3 = chry
Damat Damat1*, Roy Hendroko Setyobudi2, Juris Burlakovs3, Zane Vincēviča-Gaile4
Devi Dwi Siskawardani1, Rista Anggriani1 and Anas Tain5
...e the fiber content. A disadvantage of analog rice is its bland taste, which can be improved by adding spices and seaweed, expected to increase the functional value. This study aims to determine the effect of the addition of various seaweed pulp concentrations and spice formulations on the characteristics of analog rice. Various formulas of concentrated seaweed pulp (5 g, 10 g, and 15 g) and spice formulations (onion, garlic, ginger, turmeric, and lemongrass) ...

Nour El-Hoda Khayrat Hammad1*, Yousef Y. El-Seady3, Azza E. Hassan1, Sara T. Elazab2, Magdy S. Amer2 

...iven LIN oil (60 ml/kg basal diet) for two weeks. FCZ administrated cocks showed elevation in abnormal sperm % and significant decrease in serum testosterone hormonal levels and spermatozoa (viability and motility) which accompanied by some histopathological damage in testes and epididymis. On a molecular basis, mRNA expression of CYP17A1 and LHR genes were significantly reduced in testicular tissues of FCZ group while aromatase gene was elevated. The administ...

Sajad Ali Solangi1, Jamal-U-Ddin Hajano1*, Rehana Naz Syed1, Sohail Ahmed Otho2, Khadim Hussain Wagan1, Agha Mushtaque Ahmed2, Fahad Nazir Khoso2, Aftab Raza Jarwar3 and Suman Tarique Qazi1

Raheela Naz1*, Muhammad Aftab1, Ghulam Sarwar2, Ana Aslam1, Qudsia Nazir1, Asifa Naz3, Abid Niaz4, Farah Rasheed1, Amina Kalsom1, Nisa Mukhtar1, Sadia Sultana1, Ifra Saleem1, Arfan ul Haq1, Muhammad Arif1, Aamer Sattar1, Sarfraz Hussain5 and Muhammad Adnan Rafique6

...ity status and free from salinity and sodicity hazards. Wheat grains were analyzed for mineral contents. The results revealed that maximum grain yield was obtained from treatment T6 (Half N at sowing time + spray of 2% N after 30 & 45 DAS + half K at sowing time + spray of 2% K after 30 & 45 DAS) as compared to other treatments. Furthermore, the chemical analysis showed the maximum quantity of N, P and K in wheat grains obtained from T6. This study con...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

Noura El-Shahat Attia1*, Abd El-Khalek Ramadan El-Sheikh1, Mohamed Omia Siam2 

...l spores or mites. Blood samples were taken for hematological analysis, along with taking serum samples for biochemical analysis. Rectal temperature, heart, and respiration rates were not significantly differed between the two groups. Packed cell volume (PCV), total erythrothetic count (TEC), white blood cells (WBCs), serum glucose, total proteins and serum Zn were significantly decreased. Moreover, while copper (Cu) and iro...

Ahmad-Ur-Rahman Saljoqi1, Sumayya Amin1, Muhammad Salim1*, Taufiq Nawaz2 and Farida Anjum3

...ecommended in the IPM as safe pesticides against tomato fruit worm in tomato crop. 

...

Neda Amani1, Mehrdad Shariati1, Rahim Ahmadi2,3*, Mokhtar Mokhtari1 and Saeed Khatamsaz1

...was measured using MTT assay method. iNOS, MMP9, caspase-3, -8 and -9 expression level were evaluated by real-time PCR and the caspases activity was measured by ELYSA method. Flow cytometry was used to detect and quantify the level of apoptosis in cell lines. The data were statistically analyzed between groups using ANOVA and student’s t-test. Decreased viability was observed in AGS ...

Saba Iqbal, Asmat Ullah*, Muhammad Luqman, Hafiz Muhammad Akram, Muhammad Kashif Munir and Nawal Zafar

...hout compromising yield, sacrificing income of the farming community and evidencing sustainability in production systems. Thus, a two years field study (during 2017-18 and 2018-19) was conducted at research area of Agronomic Research Station Khanewal, Punjab, Pakistan to compare the existing traditional winter wheat (Triticum aestivum L.) planting techniques (broadcast and drill) with the water efficient planting techniques (ridge and bed planting) to evaluate...

Saima Parveen1, Altaf Mahmood2*, Ayesha Azad3, Sajid Umar4, Nosheen Shoukat6, Mirza Muhammad Arsalan Azam5, Qurat-Ul-Ain5 and Nausheen Akhtar Malik1

...hakwal, a representative sample of 70 farms with capacity of 448000 broilers was randomly selected for collection and analysis of disease data. Five years’ data of these randomly selected farms revealed highest (44.64%) crude morbidity during monsoon season followed by 23.92%, 22.12% and 17.49% for winter, spring and post-monsoon seasons respectively. The highest (14.90%) prevalence was recorded for new castle disease followed by infectious bur

Muhammad Niamatullah1, Abdur Rehman1* and Raheel Saqib2

... respect, only 100  sampled farmers were interviewed with the assistance of a well-designed structured questionnaire using simple random sampling procedure. Regression analysis as a statistical method was used to assess the impact of tube well irrigation on agriculture production, household annual income, poverty line, kinds of land, sources of irrigation and inputs used on land. The significant (P<0.05) impact of ho...

Neelam Saba and Saba Irshad*

... its association with consanguinity in congenital cataract cases was also studied. This study was conducted on 192 cases of congenital and acquired cataract presented at LRBT Hospital and Mughal Eye Hospital, Lahore (March 2016-September 2019). Children under 15 years of age (mean age=2.2 ± 1.03 years) and diagnosed with congenital or acquired cataract were included in the study. Among 192 cases of cataract 166 were of congenital and 26 were of acquired...
Hadia Gul1, Abdul Haleem Shah1, Ricardo Harripaul2, Anna Mikhailov2, Ejaz Ullah Khan3, Wasim Shah3, Nisar Ahmad3, John B Vincent2,4 and Muzammil Ahmad Khan3*
...ic factor of OCA in a consanguineous family from Pakistan. Genetic analysis was performed through microarray genotyping and homozygosity-by-descent (HBD) mapping, whole exome sequencing (for mutation identification) and Sanger sequencing (for variant segregation). Homozygosity analysis revealed a 1.2 Mb HBD region on chromosome 15 between markers rs4778147 to rs8036234 (chr15:27752745-28962131bp), which harbors the previously reported OCA2 gene. The subsequent...

Mukhlisi Mukhlisi1,*, Tri Sayektiningsih2 and Ishak Yassir1

...g, East Kalimantan. Bird sampling was done in hill karst forests and coastal karst forests. We employed a point count method by following transects. Observers walked constantly along transect and stopped every distance of ±200 m to record all sighted birds for 10-15 min. We found 89 bird species: 67 bird species were identified in hill karst forest and 33 bird species were recorded in coastal karst forests. Eleven bird species were found in both study s...

Sozan A.A. Ismaeil1*, Hassan Emam2 

...ng pigeons and Ibis. The samples were fixed and processed for the histological and histochemical examination of the liver. Pigeons were collected from the squab breeder while Ibis were collected from streets at Shariq province. The morphological findings showed that the liver of two species of birds is located in a hepato-peritoneal cavity, and each is composed of two undivided lobes, a large right lobe and a small left one which have light brown to dark red i...

Riaz Shah1*, Shaikha Hilal Al Ismaili2, Sheikha S. Al-Siaby1, Amal Mohammed Al Nasiri2, Thuraiya Hafidh Al Maskari2, Jamal AlSabahi3 and Huda Al-Ruqaishi3

...adinol were found in all samples. These 14 stable compounds could be considered as chemical markers for basil. The different regions seem to have different yield/composition.

...
Muhammad Faisal Riaz1, Abu Bakar Muhammad Raza1*, Muhammad Zeeshan Majeed1 and Talha Nazir2
...e) are small soft-bodied sap-sucking insects with about 5,000 described species worldwide. Many of the aphid species are destructive pests of a wide array of horticultural and agricultural plants. This study determined the prevailing diversity of aphids on different economically important plantations in district Sargodha. From November to April 2018-2019 and 2019-2020, about 51,000 apterous adult aphid specimens were collected from various plantations from all...

Junaid Ahmad1*, Shazma Anwar1, Anwar Ali Shad2, Sher Shah Souri1, Bibi Amina3, Wajia Noor4, Abidullah1 and Muhammad Adil1

...), seeds pod-1 (11), thousand seeds mass (38 g), biological and grain production (2960 and 777 kg ha-1) while highest nodules plant-1 (26), plant height (79 cm), protein (21.70 %), carbohydrates (60.53 %) and seed N content (3.76) was recorded with molybdenum applied at rate of 2.5 kg ha-1. Similarly in case of phosphorus application at rate of 60 kg ha-1 recorded maximum pods (30), seed pod-1 (11), thousand seed weightage (...

Muhammad Shoaib1*, Muhammad Nawaz2, Muhammad Ilyas3, Muhammad Shafique1, Imran Khan1*, Muhammad Talha Aslam1, Muhammad Sultan Ali Bazmi4, Muhammad Arshad5, Ghulam Ahmad4, Muhammad Irfan6, Muhammad Umer Chattha1 and Muhammad Umair Hassan1

...lers (PT) (364 m-2), thousand grain weight (TGW) (42.67 g), biological yield (BY) (10.61 t ha-1), grain yield (GY) (4.49 t ha-1) and harvest index (HI) (41.94%) was obtained with 150 kg ha-1 seeding rate and lowest LAI, CGR, height (88.65 cm) PT (283.11 m-2), TGW (38.30 g), BY (9.57 t ha-1), GY (3.66 t ha-1) and HI (38.66) was noted in seed rate of 100 kg ha-1. In case of seed size maximum LAI, CGR, plant height (99.67 cm), PT (348 m-2), TGW (42.67 g), BY (10....

Shoaib Hameed1, Shakeel Ahmad1, Jaffar Ud Din2 and Muhammad Ali Nawaz3*

...rown bear (Ursus arctos isabellinus) historically occupied the vast mountain ranges of South and Central Asia. Their range has shrunken significantly in the past century and they currently live in small and isolated populations. Most of their range has not been surveyed; hence information on their distribution is largely based on anecdotal information and expert judgments. The present study investigated the species’ current distribution in the Hindu Kush...

Amir Z. Allam, Hayam A. Mansour, Nermeen M.L. Malak, Heba H.S. Abdel-Naeem* 

...ion rate was recorded in samples treated with T. Therefore, addition of O into the mixture of T-O, O-CL, or T-O-CL improved their antibacterial activity. Furthermore, treatment of chicken patties with TCL, TO, OCL, as well as TOCL completely suppressed the growth of LAB (< 2 log10 CFU/g) at 0-time of examination and during the entire freezing storage. Additionally, the highest reduction rate in APC, psychrotrophic, and Enterobacteriaceae count was observed ...

Bikash Puri1, Anil K. Tiwary2, Bharata Regmi3,4, Dinesh K. Singh1, Doj R. Khanal5 and Manoj K. Shah3*

...al. A total of 220 blood samples were collected randomly from apparently healthy ruminants (cattle, buffalo, sheep and goat) and screened for Bluetongue virus (BTV) antibodies in sera using a cELISA kit. Out of 220 sera samples, 92 were positive for BTV, accounting for 41.8% prevalence in ruminants. Seroprevalence rate was the highest in Buffaloes (58.3%...

Hams M.A. Mohamed1, Mona A. El-Zamkan2* 

... of pathogenic and commensal bacteria that show a surprising capacity for adaptation to new hosts and antimicrobial resistance, resulting in the spread of infection all over the world, leading to huge health loss. A total of 100 raw milk samples collected from small scale producers, farmers and markets at Qena city, Egypt, were examined for the presence of Streptococcus species. The preliminary identification was confirmed b...

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

...and expectations on food safety and quality are increased over time as many food stuff and products are exposed to contamination by numerous pathogens concerning Salmonella species. The current study aimed to investigate the prevalence of Salmonella contributing contamination in chicken samples that were collected from broiler farms and slaughterhouses using conventional culturing, biochemical, and serological identification...

Syed Haseeb Ahmed Shah, Irfan Shahzad Sheikh, Abdul Samad, Muhammad Kamran Taj, Mohammad Masood Tariq*, Majed Rafeeq, Sumaira Fazal, Niamatullah Kakar, Sabeera Afzal and Asadullah

...trol group was kept on basal diet. AGPs (Oxytetracycline and Tylosin phosphate) were supplemented solely and in combinations with different concentration. Chicks were reared in floor pens bedded with saw dust litter, optimal husbandry conditions for broiler rearing were provided to the birds and vaccinations against Newcastle disease and Infectious bursal disease (IBD) were done at 9th, 19...

Nafisa Shoaib* and Aisha Majid Ali

... (Perna viridis) in MN assay proved to be a sensitive tool for the assessment of genetic damage.
...

G. Basana Gowda*, N.B. Patil, M. Sahu, S.R. Prabhukarthikeyan, S. Raghu, G.P. Pandi, T. Adak, C.K. Swain, S. Pokhare, S.D. Mohapatra and P.C. Rath 

...astaneum. Phosphine bioassay of different populations of T. castaneum revealed that Jagatsinghpur (Odisha, India) population had the highest LC50 value (1.104 mg/l). Further, gut bacteria were isolated and characterized through biochemical and molecular techniques. Among nine isolates of bacteria from resistant and susceptible populations, six isolates belonged to gram positive bacteria and three belonged to gram-negative. The 16S rRNA gene sequences displayed...

Hong Yin*, Dan Yang, Ji-Jie Liu, Jing-Wei Ding and Dan-Dan Cui

...ted for serum and tissue samples. Histopathological studies showed the tissue protective role of β-CM-7 in aged mice. The treatment of β-CM-7 in aged mice significantly increased the triglyceride (TG) level with decreasing high density lipoprotein (HDL) level in serum. The superoxide dismutase (SOD), glutathione peroxidase (GPx) activities and malondialdehyde (MDA) level were significantly increased in liver tissues with β-CM-7 treated. β-C...

Mirza Lena1,2, Dinda Fathia Syahramadani2, Alfi Nurrachma Gustya2, Arif Darmawan2,3, Sumiati2, Wiwin Winarsih4, Minoru Maeda5, Komang Gede Wiryawan2*  

...e treatment diets were basal diet with 0.1% different supplementations: T1= CaCO3 (w/w) (control), T2 = L. lactis at 108 CFU/g, T3 = B. licheniformis spore at 108 CFU/g, T4 = combination of L. lactis at 108 CFU/g and B. licheniformis spore at 108 CFU/g, T5 = B. subtilis spore at 108 CFU/g, and T6 = B. amyloliquefaciens spore at 109 CFU/g. At the pre-starter, the dietary combination T4 yielded lower (p < 0.05) feed conversion ratios compared to the T6 group....

Gülseren Kırbas Dogan1,*, Emin Karakurt2, Mushap Kuru3 and Hilmi Nuhoglu2

...ma reflexum, two with anasarca, and one with nasal and calvarium openings. After necropsy, samples were taken from the organs, foreseen and routine pathological examinations were performed. Following these procedures, the calves were brought to the anatomy laboratory and anatomically examined. The most important result; in the mid-section of the sections taken from the existing structure o...

Hafiz Adnan Ahmad1,2*, Muhammad Aslam2, Salman Gul2, Tariq Mehmood2 and Munawar Ali Munawar2

...le (3) followed by condensation with amines and all derivatives were obtained in moderate to good yield. All synthesized hybrids were characterized with spectroscopic techniques and further evaluated for anti-inflammatory potential. The synthesized hybrids were screened in vivo by employing carrageenan-induced paw edema method at 10 mg/kg dose. Among all derivatives, two compounds 4g and 4b displayed better anti-inflammation potential than standard drugs celec...

Ayman M. Khalifah1*, Walaa A. Kashyout1, Sara A. Abdalla2, Hassan S. Zeweil3, Soliman M. Zahran3, Tarek A. Ebeid4, Waleed M. Dosoky3 

...s the concentricity of unsaturated fatty acids (UFA) and significantly suppresses the concentricity of saturated fatty acids (SFA) compared to birds fed BD. Likewise, dietary Cr-Met supplementation improves blood traits, including a lipid profile, kidney functions, and antioxidant profile of laying quails. In conclusion, Cr-Met supplementation to laying quails diets at 0.4 and 0.6 g Cr-Met/kg under HS enhances performance tr...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...mbrane integrity (HOST assay), acrosomal integrity (NAR), viability (Live/Dead), DNA integrity (Acridine orange assay) and oxidative stress (TBARS assay). Five straws from each Gallic acid group were thawed individually in water bath at 37°C for 30 seconds. The addition of 15 µM GA to semen extender improved marginally the buffalo bull spermatozoa motility, viability and membrane...

Ramy S. Yehia1,3, Essam A. Shaalan2* and Hashem M. Al-Sheikh1 

...ls (celery, ginger and sesame) as well as influence of sublethal dose from both fungi on the larval development were also evaluated. B. bassiana was more potent than M. anisopliae in both larvicidal activity and inhibition of flies’ emergence. Blends from fungi and essential oils exhibited synergistic effect but fungi mixture produced antagonistic effect. The development of M. domestica larvae was affected by sublethal dose from fungi. In conclusion, M. ...

Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4 

...up: received 0.9 percent saline, doxorubicin group: Mice were intraperitoneal injected with doxorubicin (5 mg/kg) ithree times over a period of two weeks (dose every five days) and almond oil group: Mice were administered almond oil orally (2.26 g/kg) using oral gavage daily over a period of three weeks, one week prior to the doxorubicin injection and two weeks along with doxorubicin injection. The effects and mechanisms of doxorubicin on superoxide dismutase ...

Maskur Maskur1*, Muhammad Muhsinin1, Lestari, Tapaul Rozi1, Asnawi2 

... in Lombok island West Nusa Tenggara province locus was investigated based on morphometric traits and four microsatellites marker. Experimental ducks consisted of 75 Sasak ducks, 75 Khaki Campbell ducks and 70 Mojosari ducks. Morphometric traits were recorded and statistically analyzed, whereas genetic parameters of microsa

Kanakuntla Sandhyarani*, Dhoppalapudi Madhuri, Yadala Ravikumar 

...nd decreased alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatases (ALP), serum total proteins, serum albumin and glutathione stimulating hormone (GSH). Grossly, chalky white deposits of urate crystals on the serosal surface of pericardium, liver, intestines, air sacs, kidneys and ureters. Microscopically, tissue sections show urate crystal deposition in paren...

Nargis Sardar1*, Maria Binte Sarfraz2, Sufian Rasheed3 , AKM Rezwan Sardar4 , Fahamida Zaman5, Arsalan Rasheed6,7* 

...h, therefore, it is necessary to find a sensitive, selective as well as quick and easy technique for its determination. This review focuses on the Vitamin D investigations of electrochemical biosensors that have been conducted in recent years. According to the research, the practical use of electrochemical biosensors is attributed to the existence of UV radiation and transactivation of pharmaceutical items, food, or even hum...

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

...tive glass/collagen/chitosan composites scaffolds with osteoblast mineralization of normal rabbit bone marrow mesenchymal stem cells (rBM-MSCs) and cancer osteosarcoma cells. The non-cellular in vitro bioactivity test was performed in simulated body fluids for periods 1, 3, 10, 20 and 30 d by measuring the calcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiat...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
...of the study was fined a safe way to control the hazards of tetracycline concentrations through study the influence of simmering and frying on these residues. Quantitative determination of tetracycline residues was done using High Performance Liquid Chromatography. The results revealed that the incidence of tetracycline residues was 260 (87%) in all examined chicken meat. The average concentration values of oxytetracycline (OTC), tetracycline (TTC), chlorotetr...
Ghada Mohamed Safwat1*, Mohamed Ahmed Kandiel1, Omayma A.R. Abozaid2, Mahmoud Mohamed Arafa3, Sahar Omar Mohamed4
...ficantly increased while saturated concentrations fatty acids were significantly decreased in olive cake group. Gene expression of FAS and ACC was significantly increased in the olive cake group.
 

Naima Din1, Misbah Ashraf1, Muhammad Rizwan2*, Muhammad Babar Shahzad Afzal4, Hafiz Ghazanfar Abbas2, Farrukh Ilahi2, Amir Hameed5, Muhammad Ahsin Ayub6, Qurban Ali1 and Muhammad Farooq2,3

...rch Institute (AARI), Faisalabad in 2018-19. Observations regarding the population of A. devastans were recorded from 20 leaves per treatment at random (upper, lower, middle leaves), Physio-morphic characters viz., plant height, number of branches, area of leaf lamina, hair density on midrib, chlorophyll contents and moisture percentage were also evaluated for the tested varieties. Among the tested varieties, OK Advanta-803 was most resistant to the jassid (13...

Imtiaz Ali Shah1*, Maleeha Anwar1, Rafiullah2, Inamullah Wazir1, Muhammad Hasnain Riaz1, Abdur Raziq1, Muhammad Ijaz Ali1, Faizul Hassan1, Khalid Khan3, Yasin Ahmad4, Irshad Ahmad5, Muhammad Ibrahim Rashid5, Muhammad Tariq Zeb1* 

...or this purpose, 200 egg samples were collected. The culturing of Salmonella from egg shell and their contents was carried out according to ISO guidelines for Salmonella isolation. A total of twenty-two (22) eggs were positive for Salmonella species. The antibiotic susceptibility testing revealed that Salmonella isolates were resistant to ampicillin, amoxicillin/clavulanic acid, cefotaxime, and kanamycin while isolates were susceptible to chloramphenicol, gent...

Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2  

...ycoplasma mastitis. Milk samples (n = 579) were collected from three districts viz., Jaffar abad, Sohbat pur and Naseer abad from mastitis suspected buffaloes. The samples were collected from those animals which had abnormal milk or physical signs (like swelling, abscess, wound etc.) on udder or teats or have suddenly dropped milk production. All samples were cultured on PPLO (pleuropneumo...

Saima Mustafa1, Firdous Bukhari1, Muhammad Nazar Aftab1, Muhammad Asif1, Muhammad Amjad1, Maryam Ijaz1, Muhammad Latif2 and Furhan Iqbal1*

...s adopted to identify causative agent of dwarfism that reveled a previously reported a missense mutation (c.2011 A > G, p.Ser 671Pro) in exon 3 of COL10A1 gene. Sanger sequencing confirmed these mutations in all enrolled subjects and mutation followed Mendalian pattern of inheritance. Multiple sequence alignment by Clustal Omega revealed that domain of COL10A1 containing mutations is highly conserved. In conclusion, we are reporting a p...

Shakila Mumtaz1, Khalid Javed1, Muhammad Dawood1*, Muhammad Imran2, Asad Ali1 and Nazia Ramzan1

Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz

...e (bla-Ndm1) gene. Blood samples (5ml) of children suffering from different infections, under treatment in a teriary care hospital, were screened for blaNDM-1 gene. Blood samples of 116 patients having tested for multiple drug resistance were analyzed for blaNDM-1 gene by PCR. Sixteen samples were found to be positive for blaNDM-1 gene. The bacterial species harboring blaNDM-1 gene were 25...

Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3

...tagious disease of young salmonids and nonsalmonid fish with acute to peracute clinical forms. This study was designed to investigate the relationship between oxidative stress and cell damage due to IPNV in rainbow trout gonad cell line-2 (RTG-2). For this purpose, IPNV was inoculated in RTG-2. Then, media of IPNV infected cell and media of negative control were collected (n=3) for each incubation time (0, 8, 24, 48, 72 and ...

Qaisra Siddique1*, Sajid Abdullah1, Huma Naz2, Khalid Abbas1, Laiba Shafique1 and Qingyou Liu3

...s exposed for 60-day and sampling was done after 7-day. Results showed that the GST activity was considerably increased in L. rohita as compared to control. The GST activity was enhanced in various tissues (hepatic, brain, cardiac, gills, renal and muscle) of CPF treated fish as compared to control group. Comparison among different concentrations indicated that 1/3rd of LC50 caused greater increase in GST activity followed by that of 1/5th and 1/6th. The GST a...
Ranjhan Junejo1*, Shahabuddin Memon1, Muhammad Usman Shar2, Ayaz Ali Memon1 and Fakhar-un-Nisa Memon3
...ne (Ethyl acetate, EA) dosages, effect of date fodder, trap density, the effect of trap color and seasonal changes have been optimized. From the results, it has been revealed that the traps baited with 300 g of date fodder, captured more adult red palm weevil as compared to the traps without date fodder. In addition, 3 mg/trap/day pheromone dose and 5 mg/trap/ day EA captured the maximum number of adult red palm weevils and effective trap density was found to ...
Mohamed Ali Zayed
...as water is excessive in salt, which could reason physiological dissatisfied or maybe a death in farm animals. Three major muscles Triceps Brachii (TB), Longsimus Dorssi (LD) and Biceps Femoris (BF) samples were collected at 24 h postmortem from the carcasses of Bakri lambs. Lambs were separated according on drinking water type into two groups, the 1st group (G1); la...
Panjono1*, Endy Triyannanto2, Widagdo Sri Nugroho3, Muhammad Danang Eko Yulianto1, Bayu Andri Atmoko1,4
...erally, slaughtering the sacrificial cattle on Eid al-Adha is conducted traditionally, conventionally, and tends to be rough so that the cattle experience torture. Restrain is important in the slaughtering process by using equipment in the form of a restraining box. This study aimed to examine and identify the effect of using the portable restraining boxes (PRB) on the slaughtering efficiency and the level of animal welfare in the slaughtering process of

Jocelín Selene Sánchez Cisneros1, Braulio Alejandro Fuantos Gámez1, Martin Herrik Ramón Kane1, Laura Miranda Contreras2, Camilo Romero Núñez2* 

...e folds and wounds. Skin samples were taken for histopathology and compared with a healthy specimen of the same species and colour characteristics. The diagnosis was cutaneous collagen dysplasia suggestive of cutaneous asthenia. This is the first report of cutaneous asthenia in a baby albino Burmese python in Latin America.

Keywords | Burmese python, Histopathology, Ehlers-Danlos syndrome, Wounds, Cutaneous asthenia&...

Shahid Sherzada1,2*, Muhammad Naeem Khan1 and Masroor Ellahi Babar2

...ntification of sequenced samples was done by NCBI (98-99%) and BOLD (99%) databases. The sequence data analysis represented 15 variable polymorphic sites and 4 haplotypes. Mean haplotypes and nucleotide diversity was 0.42 and 0.0018, respectively. The mean intraspecific and intragenric K2P genetic distances were 0.2% and 1.2%, respectively. Rate of transitional and transversional substitution was 16.68% and 4.16%, respectively. The transition/transversion bias...
Fei Kong1,2, Qingjun Zhu1, Fanrong Xiao1, Jacob Mueti Ngwawa1, Hongxing Zhang2 and Haitao Shi1*
...hina. 24 stomach content samples were collected from wild-caught individuals from natural habitats, the analysis result showed that the species diet was noted to be primarily omnivorous and comprised 23 prey taxa classified into nine groups: plant (4), fish (1), frog (1), earthworm (1), insect (9), mollusk (3), shrimp (2), and other miscellaneous items.

...

Sajjad Khan*, Naila Chand, Abdul Hafeez, Nazir Ahmad 

...and 1.5% GA along with basal feed for 42 days. A completely randomized design (CRD) was followed during statistical analysis while difference in means was calculated through Tukey’s test. Results indicated that supplementation of GA at 1.5% significantly (P<0.05) improved feed intake, body weight gain, feed conversion ratio (FCR), European Production Efficiency Factor (EPEF) while no effect on livability of broiler chickens. High level (1.5%) of GA si...
Afsheen Noman Saddar1, Anila Naz Soomro2*, Sadaf Tabasum Qureshi1, Syeda Saleha Hassaney1 and Mukhtiar Ahmed Mahar2
... brine shrimp toxicity assay. Brine shrimp eggs hatching was optimized by applying three temperature levels (25°C, 27°C and 29°C), three salinities (10 ppt, 30 ppt and 35 ppt), two food demands (0.8 g and 1.6 g) and two levels of shrimp egg (1 g/l and 1.5 g/l). Five concentrations (1200, 2500, 5000, 10000 and 15000 µg/ml) of aqueous extracts of five fertility enhancing plants were tested for 24 h incubation...

El-Kholy KH1, Tag El-Din H1, Tawfeek FA2, Sara HM Hassab1* 

...trol group (received a basal diet without addition), SLAE2.5 group (received basal diet + 2.5 ml SLAE /kg diet), SLAE 5 (basal diet + 5 ml SLAE /kg diet) and SLAE 7.5 (basal diet + 7.5 ml SLAE /kg diet). Averages of ambient temperature, relative humidity, and THI during this study were 32.27±0.44°C, 71.65±0.85%, and 30.54±0.43; r...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
... handlers working in the same food premises. In addition to the sequencing of 23S rRNA gene and phylogenetic relation between S. aureus isolated from meat products and food handlers. Also, detect the antimicrobial profile and effect of two natural agents of cow milk proteins such as casein and α-lactalbumin on S. aureus isolates of chicken and beef meat and human. Bacteriological culture and PCR were used for S. aureus detection in 150 meat products (50 ...

Muhammad Salman*, Muhammad Hamayoon Khan, Muhammad Zahid, Gul Zamin Khan, Fazli Rahim and Usman Khalique

...amilies, viz., banana (Musa acuminate), persimmon (Diospyros kaki), apple (Malus domestica) and tomato (Solanum lycopersicum). The study was performed under free choice conditions by exposing all the tested fruits in the same arena to B. zonata female adults. Results revealed that tested biological parameters were significantly affected by the tested host except sex ratio. However, banana was found the most preferred host wi...

Bilal Jan1, Farida Anjum1*, Aasma Bibi1, Syed Roohul Hussain1, Abdul Ahad1, Azeem ud Deen1, Zia Ullah2 and Farrakh Mehboob3

... total of thirteen peach samples, ten from fruit orchards and three from fruit markets, belonging to five different varieties were collected and analysed. 38% of the samples were found to be non-compliant to EU-MRL for chlorpyrifos, 8% for atrazine and parathion-methyl, whereas no non-compliance was observed for FAO/codex maximum residue limits (MRLs). The designed method for the multi-class pesticide residues was standardiz...

Hassan Ali1, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1* and Muhammad Imran Hamid2

...granarium. Toxicity bioassays revealed that the extracts of Citrus reticulata L. and Solanum nigrum L. were most effective against T. granarium causing significantly higher larval mortality (35 – 40%) than other botanical treatments. Similarly, the highest concentration (15%) of C. reticulata extract exhibited maximum repellency (88%) of larvae, followed by Datura stramonium (84%) and Azadirachta indica (79%) at 24 h post-exposure. Regarding bioas

Houria Bouazzara1,2,*, Rachid Chaibi 1,2, Farouk Benaceur 1,2,3, Amira Nouioua4 and Laura Bruno 5

...er 2018 to October 2019, samples were collected on a monthly basis from four stations. Thirty-three zooplankton species belonging to four taxa have been described from the Wadi M’zi River; Copepoda, Rotifera, Cladocera and Nematoda. The following diversity indexes were used to measure the diversity of zooplankton species in the lake; Shannon Index 3.14 to 3.8, Pielou Evenness Index 0.72 to 0.84 and Margalef Index 1.08 to 1.45. The zooplankton communities...

Hazel Tamakan* and Huban Gocmen

...d by PCR. A total of 100 samples including 50 cats and 50 dogs were examined from 6 veterinary clinics. S. pseudintermedius (4%) and S. aureus (8%) were isolated in cat samples; S. pseudintermedius (56%) S. aureus (6%) and S. schleiferi (2%) were isolated in dog samples, and 3 MRSP strains were identified. Methicillin resistance of these isolates was found to be statistically significant (...

Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1* 

...factors along with serum samples from eight non-vaccinated dairy herds. The collected serum samples (n=56) were examined for detection of leptospiral antibodies using the Microscopic Agglutination Test (MAT), the gold standard serological test. The Chi-square and odds ratio analyses were employed to identify the important risk factors for leptospirosis in dairy farms. The study revealed that the seroprevalence of bovine lept...

Hilarius Yosef Sikone1*, Budi Hartono2, Suyadi2, Bambang Ali Nugroho2 

...od. Determination of the sample using two methods: purposive sampling (applied to farmer respondents) and snowball sampling (to collect respondents from traders and butchers). Samples used in the study were 115 respondents consisting of 90 farmers, ten collectors, four inter-island cattle traders, six cattle butchers, and five meat retailers. The data was analyzed quantitatively, the prese...

Asmaa Sh. Fayed1*, Safaa M. Abo El-Soud2 

...rage. After coating meat samples, we analyzed their microbial, chemical, and sensory properties after 0, 3, 5, 7, 9, and 11 days of chilled storage. GA/ zein wax sheets significantly reduced (p < 0.05) the abundance levels of total bacteria, and psychrotrophic bacteria, as well as pH, thiobarbituric acid (TBA), and total volatile base nitrogen (TVB-N), especially for 4% GA/ zein wax film. The coatings resulted in the prolongation of the shelf life of meat c...

Rafiqul Islam1, Nasrin Sultana1*, Md. Abu Hadi Noor Ali Khan2 

...e trial, blood and heart samples were collected. The serum cholesterol and triglycerides levels were determined. The heart specimens were prepared for histomorphological examination after the gross morphologic and morphometric (weight, length, and width) examination. Results indicated that the DEX treated broilers had significantly higher serum cholesterol and triglyceride levels than the control broilers. In the gross morphologic and morphometric examination,...

Tahir Munir Butt1*, Azra2, Muhammad Luqman3, Naveed Farah4 and Muhammad Yaseen3

... Punjab (Lahore & Faisalabad) were purposively selected as the targeted research area. Data were collected from randomly selected 120 vegetable growers from each district who used to do vegetable farming using wastewater. Total sample size of the study was 240 vegetable growers. Quantitative and qualitative data were collected by conducting research survey. Interview schedule as well as interview guide was used as the re...

Palwasha1, Siraj-ud-Din1 and Muhammad Fahim2*

...nowledge about good phytosanitary practices and v. inability to choose the right pesticide/fungicide for better management are some of the contributing factors behind peach decline. Such situations are common in developing countries where lack of knowledge and awareness of farmers are playing a major role in food insecurity and perhaps in implementations of SDGs. 

...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Shaukat Ali1, Muhammad Farooq Bhatti2, Azizullah1, Shafaat Yar Khan3, Abdul Khaliq4

Leila Shirani Bidabadi1,2, Mulood Mohammadi Bavani3, Fatemeh Janjani4, Abedin Sghafipour5*

Arman Sayuti1, Hamdan Hamdan2, Miftahul Jannah3, Safriadi Safriadi3, Teuku Nasri3, Tongku Nizwan Siregar2*

...e-linked immunosorbent assay (ELISA), blood samples were collected on day 0 (the day when rabbits mated) for estrogen measurement and on day 7 and day 14 for progesterone measurement. Furthermore, pregnancy examination using ultrasound was carried out on the 19th day after rabbits were mated. The average concentration of estrogen on day 0, progesterone c...

Jan Clyden B. Tenorio

...seek to keep communities safe and healthy by promoting human and environmental health through preserving animal health. As a panopticon to view the achievement of vital global health outcomes, One Health is truly a collaborative affair. The paradigm, by definition, is multidisciplinary, multi-sectoral, and integrative in nature. Thus, effective collaborations and partnerships towards understanding are integral to successful programs. Moreover, how a One Health...

Nora Faten Afifah Mohamad1, Hassan Haji Mohd Daud1, Ruhil Hayati Hamdan2, Sharifah Raina Manaf3, Ain Auzureen Mat Zin2, Nik Nur Fazlina Nik Mohd Fauzi2

...ers. The overall mean of saturated fatty acid (SFA) composition was significantly higher (p<0.05) in C. macrocephalus (48.21 ± 5.11) as compared C. gariepinus (32.15 ± 1.23) and hybrid CMxCG (37.70±0.35). Total monounsaturated fatty acid (MUFAs) composition of C. macrocephalus, C. gariepinus, and hybrid CMxCG was 32.14 ± 5.86, 45.24 ± 3.21, and 30.89 ±0.80, respectively, where pal...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...y identify infectious bursal disease (IBD) virus (IBDV) from naturally infected native chickens. Thus, bursa specimens were collected from two IBD vaccinated native broiler chicken flocks showing clinical signs, mortality and lesions of IBD in this study. The collected bursae showed pathological changes related to those of IBD infection. Furthermore, the isolates were molecularly character...

Muhammad Hanif Khan1, Syed Muhammad Suhail2, Hayaz uddin1, Aitbar Khan3, Rashid Ahmed Magsi3, Rajwali Khan2*, Iftikhar Ahmed2, Asim Ijaz2 and Khalid Khan2

Ali Zohaib1*, Muzzammil Hussain1, Iftikhar Ahmad1, Mushtaq Ali2, Tahira Tabassum3 and Adnan Bashir1
...atic, Chenab Basmati, Kissan Basmati, Punjab Basmati, Noor Basmati and NIAB Basmati 2016) in Gujranwala rice-wheat cropping zone. Among all varieties, NIAB Basmati 2016 produced the greatest plant height. However, Kissan Basmati and NIAB Basmati 2016 matured earlier while Super Basmati matured at last. The highest productive tillers and grains number per panicle were produced by Super Basmati and Chenab Basmati, respectively...
Ilvir Khabibullin1*, Ruzel Khabibullin1, Irina Mironova1, Lyalya Musina2, Elmira Akhmadullina1, Victoria Morozova1 
...rs had approximately the same indicators of physical endurance, by the end of the experiment, the swimming time of the experimental group animals was significantly longer. The animals receiving pantocrine turned to be stronger. Their interval time increased by 267 seconds (Р≤0.05) while the swim time of animals consuming may chang rose by 96 seconds (Р≤0.05) compared to the control. Male hamsters consuming lemongrass tincture showed the maximum efficie...
Ni Putu Vidia Tiara Timur1*, Maria Maliga Vernandes Sasadara2, Purwanta1, Anak Agung Jayawardhita3
...it was confirmed that no saliva was re-accumulated.
 
Keywords | Salivary mucocele, Surgery, Treatment, Local dog, Bali
...
Hanan A. Abo-State*, Mosaad M. El-Monairy, Yasser A. Hamouda, Hussein M.A. Hassan
...g fish-1) were fed the basal diet contained 30.36% crude protein and 3879 kcal kg-1 gross energy with different levels of PSO (0.0 (control), 25%, 50% and 75% from the source of vegetable oil that was added to the basal diet). Fish (300 male) were distributed randomly into four groups (4 groups x 3 replicate x 25 fish of each). All groups were fed diets twice a day during the trial period (56 day) at 5% of body weight for th...

Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain

... by immunodot blotting assay. The appearance of the dot on spotted position confirmed the specificity of anti-HA antibodies. The titer of antibody produced in immunogenic mice was determined through ELISA. The highest geometric mean titer of HA-specific serum IgG, which was greater as compared to the control.

...

Evrim Sönmez

...eds decreased. It can be said that different doses of B. bassiana are quite effective on the mortality rates of A. obtectus adults, the number of eggs they lay, and especially the number of adults emerged from beans.

...
Maha Khalil1, Gamal Shams2, Hosny Abdel Fadil2, Nagah Edrees2, Mostafa Abonorag1, Nasser El-Sabbagh3, Eman A. Ahmed4*
...irds were fed either a basal diet (control), basal + 1mg/kg AFB1 (AFB1 diet), basal+ 500 mg/kg GSPE (GSPE diet), or basal+ 1mg/kg AFB1+ 500 mg/kg GSPE (AFB1+GSPE diet) for 5 weeks. Biochemical parameters, lipid peroxidation, pro-inflammatory cytokines, and histopathological investigations were assessed. GSPE supplementation alleviated AFB1-induced hepato...

Moh Zali*, Nur Ihsan, Bambang Ali Nugroho, Kuswati, Umi Wisaptiningsih Suwandi, Riszqina, Suyadi

... local breeders in Pamekasan, Indonesia, known as a centre of Sonok cattle breeders. To examine the interaction effects, we used an IBM SPSS Statistics 25. Our study suggests that age, education, long-time breeding experience, family dependents are not related to cattle business activities. Moreover, we find that number of livestock is positively and significantly associated with cattle business activities. The theoretical and practical implications are discus...
El-Kholy, K.H.1*, Tag El-Din H.1, Seham N.E. Seleem1, A.M. El-Shhat2
...duckling, liver and yolk sac, leg length, relative leg length, and yolk free body weight. However, such interactions were significant for hatchability and Tona Score. The highest values of leg length and duckling body weight/duckling length ratio produced from eggs dipped in nutritive solution were recorded at 17 and 31 d of embryonic ages, respectively. According to the results, it can be concluded that in-ovo dipping in 0.15% of SV solution, especially on da...
Khaled El-Kholy1*, Yaser Rizk2, Mohamed El-Helbawy1, Eman El-Said1
...mpare the effectiveness, safety and adverse effects of clove oil with those of salbutamol drug on growth performance, carcass characteristics and some blood parameters for growing rabbits. Forty New Zealand White (NZW) rabbits aged 6 weeks and weighing 950±30 g g were equally and randomly distributed into five groups which containing eight rabbits in each. Rabbits were injected once-weekly for all experimental period ...
Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...airy cows. A total of 60 samples of cow’s whole blood were collected and extracted and DNA isolated. DNA amplification was carried out using primer Forward (BLAD_F) 5’-TCAACGTGACCTTCCGGAGG-3’ and Reverse (BLAD_R) 5’-CCCAGATTCTTGACGTTGAC-3’. Amplification of the CD18 gene segment on chromosome 1 exon 2 with a target length of 106bp has been successfully carried out with a success rate of 100%. RFLP analysis of NCOI cutting restrict...

Amal Hamad1, Ashraf M. Abu-Seida2*, Faisal A. Torad2, Nahed S. Thabet3, Shabaan M. Gadallah1

...as carried out by paired samples t-test. Unlike Nalbuphine HCl, Fentanyl citrate and Tramadol HCl, Meloxicam induced better analgesia, no significant changes in total leukocytes, neutrophil, eosinophil, lymphocyte and monocyte counts, serum levels of total protein (TP), albumin, urea, creatinine, creatinine clearance and serum enzymatic activities of alanine transaminase (ALT) and aspartate transa

Shewangizaw Wolde1,2*, Tadele Mirkena1,3, Aberra Melesse1, Tadelle Dessie4, Solomon Abegaz5 

Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2* 

...s) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-depen...

Nzube Thaddeus Egboka1*, Olajire Fagbola2, Ugochukwu Nnamdi Nkwopara1, Nnaemeka Henry Okoli1, Akaninyene Isaiah Afangide1 and Tochukwu Victor Nwosu3

...es and soil depths. Soil samples were collected from some fallow, cassava and pineapple fields in Ibadan and Ikwuano areas of Nigeria at 0-15, 15-30 and 30-45 cm depths and analyzed in the Laboratory. Spore densities of AMF varied significantly (P > 0.05) between the fallow and cultivated (cassava and pineapple) land use types in both locations. Across the soil depths, however, AMF spor...

Magdy M. Fahmy1, Nisreen E. Mahmoud 1*, Mohamed R. Mousa2, Manal M. Zaki3, Elshaimaa Ismael3, Mai Abuowarda1 

...ponding fish farms. Fish samples together with water samples were collected from 3 affected farms and 6 locations along Manzala Lake. Field observations and physicochemical analysis of water revealed deterioration of water quality indicating marked pollution. Parasitological examination of fish samples showed high total prevalence of 68.18% with 10 different ectoparasite species (4 Crustac...

Nazakat Nawaz1, Nasir Mahmood Cheema2*, Malik Muhammad Yousaf3, Muhammad Jahanzaib1, Mubashir Ahmad Khan1 and Muhammad Munir4

...to fungal attack. At the same time, it is 10 to 15 days earlier than check varieties i.e. BARD-479 and Golden. PG-1090 has been approved by concerned authorities as a new variety with the name NARC-2019 for cultivation in rain-fed as well as irrigated areas of Pakistan.

...

Abid Hussain1*, Saifullah Khan2, Shamas Liaqat3 and Shafiullah4 

...ished sources. Moreover, salient outcomes of a multi-stakeholder discussion on mountain specific innovative solutions for potential up-scaling in Pakistan have also been highlighted to cover the opinion of technical experts. The data presented herein is important for development of evidence based policy and designing development projects in agriculture and related sub-sectors in Gilgit-Baltistan and Chitral regions of Pakistan. The regions are unique in topogr...

Mudssar Ali1*, Muhammad Awais Ahmad1, Asif Sajjad2 and Shafqat Saeed1

...east. In Apidae, Apis dorsata was the most abundant followed by Lasioglossum sp (Halictidae) while Xylocopa sp. was the least abundant bee species. The foraging behavior in terms of visitation rate proved A. dorsata and Lasioglossum sp. as the most efficient pollinators followed by E. laetus. Open pollination resulted in higher seed weight per umbel (4 times), total seeds per umbel (4 times), umbel diameter (1.5 times) and u...

Adriana Cervantes-Noriega1, Norma A. López2, Alfredo Estrada-Angulo2, Beatriz I. Castro-Pérez2, Elizama Ponce-Barraza2, Gilberto López-Valencia1, Alberto Barreras1, Alejandro Plascencia3* 

... the effect of filtering-sanitization of water offered as drink water on growth performance, dietary energetics, carcass traits, and diarrhea frequency in finishing lambs. Lambs were fed with a cracked corn-based high-energy diet during 89-d. Treatment consisted in filtering and sanitization of raw dam water qualified as “clean and safe” comparing it as follows: 1) dam water (R...

Doaa Khairy1, Mohamed Ali Osman2 and Fatma Abdel Mohsen Mostafa1*

...NPK, chlorophyll Aand B, salicylic acid, phenols and phenylalinine enzyme (PAL) in tomato leaves were significantly (P<0.05) induced by plant extracts mixture. Phytochemical analysis of aqueous neem leaf extract demonstrated the occurrence of a number of flavonoids (21) and phenolic compounds (23) including Hespiridin, Naringin, Rosmarinic acid, Pyrogallol, Salicylic acid and Gallic acid which are implicated in plant preservation and hence induce strength a...

Hyun-Ju Cho1, Eun-Jae Lee2 and Shin-Jae Rhim3*

... in urban areas are necessary and should be considered in urban planning for the conservation of biodiversity.

...

Aymen Mabrouk*

... volatile oil of Nigella sativa seeds, against the deterioration of blood indices by lead (Pb) has never been studied. Therefore, the present study was carried out to evaluate the possible beneficial effect of TQ against Pb-induced hematological changes. Adult male Wistar rats were treated with Pb (2000 ppm of Pb acetate in drinking water) and/or TQ (5 mg/kg/day, per os) for five weeks. Results obtained clearly showed that Pb intoxication significantly decreas...

Ji Xu, Zhonghua Liu, Zhenhai Cui and Wenhai Zhao*

...gate the effect of gross saponins of Tribulus terrestris (GSTT) on chondrocyte injury induced by IL-1β and its possible mechanism. Chondrocytes were isolated from rat knee joints and cultured. Chondrocytes were treated with IL-1β, and chondrocytes were treated with different concentrations of GSTT. ELISA was used to detect the levels of IL-6, TNF-α and IFN-γ. Flow cyt...

Sheng Dong1*, Ning Li2, Jiawei Zhang2 and Ting Wang2

...hod and colony-forming assay were taken to detect cell proliferation ability; flow cytometry was adopted to detect apoptosis rate; qRT-PCR method was used to detect the expression of KCNQ1OT1; Western blot method was used to detect Nephrin, GRP78, GRP94, and Caspase 12 protein expressions. We found that DS enhanced the proliferation of high glucose-induced podocytes (P<0.05), increased the number of clones formed (P<0.05), reduced the apoptosis rate and ...

Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr 

...lves were studied. Blood samples were taken from 3 calves (128.33±7.58 kg and ageing 6 mo.) and 10 bulls (400±37.5 kg and 24-25 months of age) and semen samples from bulls. Semen was collected once a week from all bulls which were divided according to semen quality to high and low fertile bulls (5 for each). Reaction time and semen characteristics were determined. Blood from bulls and calves, and seminal plasma...

Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2 

...vey method and purposive sampling to determine the respondents. The observed variables were breeder profile, maintenance management, number of KBC, actual population (Na), effective population (Ne), and inbreeding rate. The total population of KBC in Nagari Batu Bajanjang, Tigo Lurah District is 1960, with an actual population of 610 chickens, an effective population of 600 chickens, and an inbreeding rate of 0.08%. The result showed the farmer’s profile...

Taiwo Oladoye Akande*, Dayo Johnson Ogunyemi, Priscilla Funmilola Okunlola, Emmanuel Owolabi, Odetayo Olakanmi 

Rana A. Ali1, Abd-Elraheim A. Elshater2, Heba A. Mohammed3*, Mahmoud Elshazly4 

...er the experiment, blood samples were collected for biochemical analysis. Also, the pancreas was dissected for histopathology. Results: biochemically, there was a significant increase in hepato-renal enzymes, cholesterol, glucose, and insulin in the STZ group, while, the other treated groups showed an improvement in such enzymes. Histopathology, STZ induced necrosis, and degenerative changes with vacuolation. Treated groups displayed moderate to mild vacuolat...

Amira M. Refaie1*, Manal S. Mohamed1, Enayat H. Abo El-Azayem1, Sherein H. Mohamed2 

...al control group fed a basal diet without AA (T1), a positive control group fed T1 diet plus 0.1% Bacillus subtilis and Bacillus licheniformis (T2; Pro.), then the 3rd, 4th and 5th were fed the basal diet supplemented with 0.05%, 0.10% and 0.15% AA, respectively and abbreviated as 0.05% AA (T3), 0.10% AA (T4) and 0.15% AA, respectively. The trail was ended after pregnant period and nursering kits till weaning. The results in...

Samah Samir, Amal Awad*, Gamal Younis 

...overnorate. Organ tissue samples from liver, kidneys, gills, skin and spleen were examined bacteriologically for the presence of E. tarda; and confirmed by PCR targeting gyrB1 gene. Disk diffusion was used to test their antimicrobial susceptibility, and PCR was used to screen E. tarda isolates for the presence of four virulence-related genes (cds1, edwI, qseC, pvsA). In addition, the ability of E.tarda to form biofilm was tested by tube test. E.tarda was detec...

Seok-Jun Son1,3, Sung-Kyung Lee1, Jung-Woo Oh1, In-Kyu Kim2, Dal-Ho Kim2, Bo-Ra Hyun1 and Jung-Hoon Kang1,*

...abited island in the Chilsan Archipelago off the west coast of the Korean Peninsula. GPS tracking was used to identify their autumn migration route and their home range and habitat type in breeding (post-fledging/pre-migration stages) and wintering sites (post-migration stage). The two egrets crossed the East China Sea to Taiwan. The signal for one bird was lost, whereas the other rested and then moved to the Philippines for winter. The home ranges of the two ...

Ejaz Ali and Nageen Hussain*

...e to a higher rate of consanguinity and Hearing Loss (HL) is one of them that affects more than 466 million people worldwide; will increase to 900 million by 2050. GJB2 mutation is one of the main causes of hearing loss in different populations, including Pakistan, that encodes a gap junction protein involved in the homeostasis of the inner ear by recycling potassium ion. This research aimed to find out mutations in the GJB2 gene and its protein structure. Bot...

Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1

...d in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 109 ml-1 ): 2.66 ± 0.10 versus 3.61 ± 0.11, P <0.001; total sperm (× 109): ...

Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1

...d in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 109 ml-1 ): 2.66 ± 0.10 versus 3.61 ± 0.11, P <0.001; total sperm (× 109): ...

Mariam Arain1, Asghar Ali Kamboh1* and Muhammad Javed Arshed2

..., Control that offered basal diet and Se supplemented group that offered basal diet + selenium yeast (0.15mg/kg BW). In basal diet concentrate was fed at the rate of 2% body weight while roughage (hay)was given as ad-libitum. The dietary treatments were continued for 2 months. The results showed that microbial count (E. coli and Lactobacillus) was higher (p<0.05) in goats offered ba

Jumshaid Iqbal1, Muhammad Sharif1*, Muhammad Nadeem Suleman2, Muhammad Saeed3, Fawwad Ahamd1, Asghar Ali kamboh4, Tugay Ayaşan5 and Muhammad Arslan3

Tiyyabah Khan1, Hafiz Azhar Ali Khan1*, Muhammad Rizwan Khan1, Muhammad Umer1, Adnan Akhter1 and Waseem Akram2

...ional insecticides (spinosad, thiamethoxam, indoxacarb and imidacloprid) against Pakistani strains of Cryptolestes ferrugineus (rusty grain beetle) and Tribolium castaneum (red flour beetle), and tested whether insects’ mortality could control fungal contamination in stored grains or not? Bioassays were performed on wheat grains artificially infested with C. ferrugineus or T. castaneum along with spores of Aspergillus ...

A. Samy1*, H.M.A. Hassan1, Fatma T.F. Abd-El Ghany2, Shama H. Morsy2 

...ive diets containing the same proportions of all nutrients were made. The first control diet (without any additives); curcumin (CE) or garlic extract (GE) at concentrations of 200 or 400 ppm were added to the other diets. The results revealed that adding curcumin or garlic extract to growing rabbit diets boosted significantly meat crude protein (P<0.05) while, decreasing moisture and fat levels. Digestibility coefficients of CP, CF, and EE, as well as the n...

Ishrat Younus1,2 and Afshan Siddiq1*

...lign: justify;">Raphanus sativus L. Var. caudatus belongs to Radish plant has been reported for analgesic potential thus it might have antipyretic potential. The present investigation was undertaken to evaluate analgesic and antipyretic activities of ethanol extract of Raphanus sativus var. caudatus in albino mice. The analgesic and antipyretic activities were determined at three different doses (50, 100 and 200 mg/kg) using...

Honnakerappa S. Ballari1 and Shashikant S. Udikeri2*

...g pyrethroid, ogranophaopsahte and carbamate molecules monitoring was exercised using populations of H. armigera collected from different agroclimatic situations in Karnataka state, India. An abstinent to tremendous resistance was documented for synthetic pyrethroids, carbamates and organphosphates insecticide despite under use of these molecules over decades. Raichur populations comprehend high level of resistance to Pyrethroids with LC 50 121.63 ppm to cyper...

Asmaa S. Mohammed*, Ahmed F. Abou-Elnaga, Ahmed I. Ateya, Mohammed M. Fouda, Ragab A. Darwish, Usama A. Abou-Ismail 

... 25-hydroxy vitamin-D to safe levels and decreased mortality rate of kits. Furthermore, the exposure to 8 hours of UVB radiations in particular improved reproductive indices including litter weight at weaning and Kits’ weight gain from birth to weaning, and up-regulated the relative expression levels of FSHR and ER-α genes. Therefore, based on the multiple measures approach used to evaluate the effect of UVB light on behaviour, reproductive and hea...

Mohamed Mohamady Ghanem1*, Yassine Mahmoud Abdelraoof1, Abdelghany Hefnawy Abdelghany2, Eman Abdelhamid El-Ebissy3, Ahmed Ragab Askar4,5, Attia Ahmed Eissa6  

Taghreed Mohamed Nabil1, Randa Mohamed Hassan1, HebatAllah Hamdy Mahmoud2, Mohamed Gomaa Tawfiek2, Usama Kamal Moawad1* 

...r concentric layers; mucosa, submucosa, muscularis, and serosa but lacks lamina muscularis mucosa. The lamina propria submucosa contained immune competent cells and tubular glands in all portions of the oviduct except the infundibular funnel and vagina that devoid of any glands. The neutral mucopoly

Xiaopeng Tang1*, Kangning Xiong1, Peng Peng2 and Dun Deng2*

...than 13.55%) may be used safely in commercial broilers diets.

...

Hosny Kesba1, Ashraf Suloma2, Samy Sayed3*, Abdullah Abdel-Rahman1 and Shaimaa Diab1

...5 days after planting in sandy loam or sandy soils. Each irrigation source was applied daily at 150 mL/pot. The STP source was more suppressive to nematode development than ITB irrigation source in the two inoculation times of treatments either in sandy soil or sandy loam soil. Also, the produced eggs were highly influenced by the STP source achieving ha...

Muhammad Tahir Jan1,2, Mushtaq Ahmad Saleem3,*, Muhammad Binyameen1,* and Sarfraz Ali Shad1

.... Similarly losses compensation was 4.5 and 9.1% higher in long duration genotypes as compared to short duration genotype in 1%TL and TL2, respectively. It was concluded that E. vittella can cause 22-24% fruiting losses if the pest is not controlled. Plant protection measures should therefore be taken before the critical tolerance period.

...

Majid Hussain1, Syed Makhdoom Hussain2, Razia Iqbal3, M. Mudassar Shahzad4,*, Syed Zakir Hussain Shah3, Afia Muhammad Akram4, Nisar Ahmad5 and Muhammad Zubair ul Hassan Arsalan2

...s mrigala fingerlings. Basal diet was supplemented with 0%, 1%, 2%, 3%, 4% and 5% CA resulting in the formulation of six experimental diets. Ten fingerlings were stocked in tanks in triplicate for each treatment. Feed was given at 5% live wet body weight of fingerlings for 90 days. Results showed that diet acidification with CA significantly (p< 0.05) improved the mineral absorption, carcass composition and hematological indices of C. mrigala fingerlings co...

Amira M. Nowier1, Hassan R. Darwish2, Sherif I. Ramadan3, Nadia A. Abo El-Maaty2 and Othman E. Othman2*

... hundred and sixty blood samples were collected for DNA extraction; 74 from Zaraibi, 41 from Damascus, and 45 from Barki breeds. Two genotypes, TT and TC, in the α-lactalbumin gene were identified using single strand conformation polymorphism. DNA sequencing resulted in one non-synonymous MT163744: g.128T>C SNP (TCG Ser> CCG Pro) at the sixth nucleotide of α-lactalbumin gene exon III. Barki breed showed the highest heterozygosity (0.260) and ...

Lin Lu

...chanism of Hedyotis diffusa polysaccharide (HDP) on apoptosis of thyroid cancer cell line. TPC-1 cells were treated with HDP (final concentration 1 mg/mL, 2 mg/mL and 4 mg/mL), PI3K/AKT signal pathway inhibitor LY294002 (20 μmol/L) and LY294002 + HDP (4 mg/ml HDP and 20 μmol/L LY294002). Cell viability and apoptosis rate were detected by MTT and flow cytometry, respectively. The expression of Cyt-C, Bcl-2, Bax, cleaved...

Xiuli Wang1, Cuilan Hu2 and Sen Ye3*

...hrombolysis group at the same time point. At the same time, the infarct volume of rats decreased in both 4.5h thrombolysis group and 6h thrombolysis group. On the 3rd and 7th postoperatively, compared with the acute infarction group, 4.5h thrombolysis group and 6h thrombolysis group showed increased microvessel density which grew gradually over time, while brain waves tended to flatten. RT-PCR results showed that the infarct...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

...uitable for non-invasive samples are important to wild population investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on which, genetic diversity and population structure of three populations of M. thibetana were estimated. Our results showed the novel SNP loci were polymorphic and bi-allel...

Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5

...iduals in a Pakistani consanguineous family by Sanger sequencing of UROS gene to identify potential pathogenic sequence variants. CEP patients were identified using successive clinical tests. Blood samples of patients were collected and processed for genomic DNA extraction followed by Sanger sequencing to identify pathogenic mutations in UROS gene. Sequence analysis revealed a pathogenic missense mutation (c.935T>C [p. L2...

Abdul Malik Ahmed, Bhargav Bhushan Nath, Ayub Ali, Jiten Sarma, Bipul Phukan, Imtiaz Ahmed* and Jyotirmoyee Das

... Brahmaputra Valley of Assam. A total of 458 specimens comprising of 161 males and 297 females with total length (TL) 7.8 to 15.6 cm and total weight (TW) 5.01 to 38.19 gm were collected monthly basis from August 2017 to July, 2018 from Thekera beel (wetland) of Morigaon District of Assam. The length-weight relationship of Gudusia chapra was found to be W=0.01L2.897, W=0.01L2.9165 and W=0.01L2.9214 for male, female and poole...
Saeed Akram Khan1, Shahid Sherzada1*, Muhammad Ashraf1, Durr-e-Shehwar1, Sonia Iqbal1, Usman Atique1,3, Farzana Abbas1, Sohail Ahmad2 and Muhammad Sultan Haider4
...al development, and yolk sac absorption induced at 4 different water temperatures viz. 26, 29, 32 and 34 ºC. C. mrigala displayed the maximum release of eggs, fertilization and hatching rates at 29 ºC. The maximum number of eggs were observed in 26oC whereas the lowest were obtained in 34oC. 85020.48). However, the temperature exceeding 30ºC not only increased the cell growth but significantly decreased the hatching time. On the contrary, temper...

Muhammad Altaf1* Arshad Mahmood Abbasi2, Muhammad Shoaib Amjad3, Sadia Naseer1 and Muhammad Umair4

Fan Xu1,2*, Cheng Wu3 and Su Zeng1

...provide a basis for drug safe use in the clinic. PGE1 lipid microspheres (lipo-PGE1) and alprostadil dried emulsion (DN-PGE1) were compatibility with different volumes of 0.9% sodium chloride and 5% glucose, respectively. After the compatibility of different concentrations, the changes of pH, particle size distributions, the particles greater than 5μm and encapsulation rate of the compatibility solutions were observed within 8 h by using electronic acidimet...

Riffat Sultana1*, Samiullah Soomro1 and Chuan Ma2

... 1914 were identified as same species i.e. C. (Chrotogonus) h. homalodemus (Blanchard, 1836) and C. (Chrotogonus) t. robertsi Kirby, 1914 respectively. Their lineages indicating negligible genetic divergence and very close resemblance with 99.85% identity between C. (Chrotogonus) h. homalodemus (SWF and LWF) against RID-MMV2XTNY114 and 99.85% identity between C. (Chrotogonus) t. robertsi (SWF and LWF) against RID-MMVW0MTF114. Finally, we discuss the possible t...
Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
...ce of NDV. A total of 80 samples were collected from eight different poultry farms located around Faisalabad city with ten samples from each farm viz. Khurrianwala road, Shahkot road, Faisalabad road, Satyana road, Jhumra road, Nankana sahib road, Lahore road and Jaranwala road. Homogenate from house flies was first ha...

Huahua Fang, Daquan Zhou and Xiaoyu Dong*

...re estimated. Ninety-one samples were collected from March to June 2019 through trawl nets in the Yellow sea area (36°27′02″N 121°08′68″E). The length-weight relationships of Lepidotrigla microptera, Liparis tanakae and Trachidermus fasciatus can be expressed as WL.microptera = 0.0123L2.92, WL. Tanakae = 0.0848L3.46, WT. fasciatus = 0.067L3.06, respectively. The values of exponent b ranged from 2.92 to 3.46 for these three speci...

Xiaopeng Tang1*, Kangning Xiong1 and Rejun Fang2*

...ls challenged by lipopolysaccharide (LPS). Cells were treated with: (1) EGF (0 ng / mL) + LPS (0 μg / mL) (Control group), (2) EGF (100 ng / mL) + LPS (0 μg / mL) (EGF group), (3) EGF (0 ng / mL) + LPS (1 μg / mL) (LPS group), and (4) EGF (100 ng / mL) + LPS (1 μg / mL) (EGF+LPS group) for 24 h. The gene expression of glutamine transport protein Na+-dependent neutral amino acid transporter (ASCT2) and protein expression of ASCT2 were measured. The ...

Ahmed M. Darwish1*, Hassan R. Darwish1, Dalia M. Mabrouk1, Mohamed A. Abdelhafez1, Ahmed M. Abdel-Salam2, Ibrahim E. Mohamed3, Ibrahim M. Farag1 

...y and Damascus goats. 80 samples of blood and milk were collected from farm animals in Sakha, Egypt. The potential of hydrogen (pH), fat, protein, lactose, and solid not fat (SNF) were determined by biochemical methods in all milk samples. Different genotypes of β-LG and LEP genes were detected by single-strand conformation polymorphism (SSCP-PCR), and then validated by sequence analysis. The results of SSCP-PCR showed ...

Qi Zhao1, Yuanyuan Hou1, Xiangdong Jin1 and Chen Zhang 2,*

...the clinical effect of mesalazine combined with bifid triple viable (BTV) on ulcerative colitis. One hundred sixty patients with ulcerative colitis were divided into the experimental group accepting mesalazine combined with BTV and the control group accepting mesalazine alone. It was found that the overall treatment effective rate was 93.75%, in the experimental group as against 77.50% in ...

Kafeel Ahmad*, Muhammad Siqaf, Gul-E-Rana Abid, Ramla Somayya and Umama Qasim

...ates recovered from fish samples were screened phenotypically for extended spectrum beta lactamases and metallo beta-lactamases production by disc diffusion method using the antibiotics cefotaxime (30 µg), ceftazidime (30 µg), cefepime (30 µg), amoxicillin-clavulanic acid (30 µg), imipenem (10 µg) and meropenem (10 µg). Three extended spectrum beta lactamases genes (blaCTX-M, blaSHV and blaOXA-10) and one metallo beta-lactam...

Nahla Hamada Magd Khalil1*, Ihab Mahmoud Helal2, El-Desoky Hassan Ibrahim Dorrah1, Soad Ahmed Soliman Ismail3 

...g 

...

Stephen Chijioke Emencheta1, Anthony Amaechi Attama2, Ezinwanne Nneoma Ezeibe1, Adaora Angela Agubata1 and Ebele Benedette Onuigbo1*

...RI) was assessed. Spot assay was done to determine lytic action of the bacteriophage on the lawns of the Escherichia coli. The phenotypic and biochemical tests confirmed Escherichia coli isolates. There was metallic green sheen on EMB agar. The isolates were citrate negative and indole positive. The isolates were found to be resistant to amoxicillin (100 %), meropenem (100 %), and ceftriaxone (100 %). The Multi Antibiotic resistance index (MARI) of the isolate...

Aml M. Ragab1*, Maha R. Basyoni1, Enas A.I. Khoris2, Nadia A. Abd Elghany3 

...nbsp;

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Azra*, Muhammad Awais, Alam Khan and Muhammad Shoaib

...omestic credit, national savings, lagged investment (IV), imports, and the real exchange rate were found to be the major factors affecting investment in Pakistan. Among all, the savings coefficient is positive and insignificant because of savings in Pakistan had no healthy pattern. Exchange rate has adversely affected the investment because of unidirectional causa<...

Nabila Khan1, Imran Ahmad2 and Muhammad Bilal Sadiq1*

...t extraction parameters; sample to solvent ratio (1:20-1:40 w/v), solvent concentrations (20-80%, v/v) and time (10-30 min), whereas, TPC and DPPH were used as response variables. At optimized extraction conditions (40 ml/g, 22.5 min and 80% of ethanol), the experimental values for TPC and DPPH inhibition were 110.17 ± 3.44 mg of GAE/g of extract and 87.45 ± 1.28%, respectively. For DPPH inhibition assay, IC50 ...
Sajad Ali1*, Naeem Ur Rehman Khatak1, Shahab E. Saqib2 and Saleem ur Rehman1
...tudy area. A multi-stage sampling technique was employed. Furthermore, to estimate wheat productivity, differences in wheat productivity and socio-economic factors between owner and tenant wheat growers, a log-log regression model and an independent unpaired sample t-test was used in conjunction with face-to-face interview schedule. In addition, a dummy variable was added to the production function to analyze the impact of t...

Albina Gabdrakhimovna Darmenova1, Samat Ravkhatovich Yusupov2, Abzal Kenesovich Kereyev1*, Mirzabek Gashimovich Zukhrabov2, Yerbol Maratovich Sengaliyev1, Dosmukan Ermuhkanovich Gabdullin1, Berik Garipullievich Satybaev1 

Alaa Jaheen*, Noha Salem, Mohamed El-sherif 

...sical examination. Blood samples were collected for hematological profile and estimation of serum concentration of total antioxidant capacity (TAC), malondialdehyde (MDA), C-reactive protein (CRP), serum zinc, copper concentrations, total protein, albumin, globulin values, triglycerides, and cholesterol concentrations. The most consistent hematological alteration was elevated eosinophils in eczema-affected horses. The oxidant-antioxidant status showed a signif...

Wael Felefel1*, Mohamed EL-Beskawy2, M. F. El -Dakroury3, Mohamed Morsi Elkamshishi4, Eman Sayed Mohammed5 

...ramectin was found to be safer where it improved kidney and liver function tests in addition to the velocity of killing ticks with injection.

Keywords | Doramectin, Fipronil, Hard Ticks, Stray dogs, ROC curve  

...

Ibrahim Samir Abd El-Hamid1*, Wafaa Adel Abd Fouda1, Hesham Attia Shedeed1, Safaa Ali Mostafa1, Ahmed Mohamed Elbaz2, Salah Abo Bakr2, Baliegh Hamdy Mosa1, Ali Saber Morsy1, Amal Mohamed Hasan1, Khamis Refaay Emam3 

...oalgae N. oculata / kg basal diet. The 3rd treatment (Tr3), received 10 g of microalgae N. oculata /kg basal diet during experimental period. Blood samples were monthly collected from all animals to determine some blood biochemical constituents, blood minerals and hormonal profiles. Reproductive and productive performance were evaluated. The results showed that total protein (TP) and globu...

Mubashar Ahmad, Roheela Yasmeen*, Aisha Waheed Qurashi, Muhammad Hassan Ali Shah 

...of contaminated food. To save animals formation of Zoos is years old practice. The animals are placed in captivity for recreation, education and economic benefits. The physical needs of animals are even met in captivity however the conditions of confinement and exposure to humans can result in physiological stress. The stress response consists of the suite of hormonal and physiological reactions which are helpful in an animal’s survival. Cortisol levels ...

Tehseen Ali Jilani1*, Muhammad Saleem Jilani2, Javeria Sherani3, Kashif Waseem2, Muhammad Sohail Khan2, Hasnain Saleem3, Abdul Manan2, Rashid Jawad2 and Sami Ullah4

Waqar Khan1, Muhammad Atif Khan1, Waseem Abbas1, Muhammad Shakeel1, Hammad Uddin2 and Bismillah Shah1*

... on around 200 specimens sampled from various regions of Pakistan during 2019–­2021. The study revealed the presence of two species, Exitianus nanus (Distant) and Exitianus indicus (Distant) in the region. Their morphological descriptions, an identification key, and photographs are given. Specimens of all examined materials are deposited at the department of Entomology, The University of Agriculture, Peshawar, KP, Pakistan.

...

Masudul Haq Wani and Arshad Bhat*

...ify;">The cultivation of saffron started around three or four centuries back in Arabia and Spain, afterwards its cultivation spread to Iran, Sweden and India. In India, around 5,707 ha. of land are under its cultivation with annual production of around 16,000 kgs. The state of Jammu and Kashmir ranks at top of the list of saffron growing states in India, with district Pulwama at slot one. Out of the total 5,707 ha. of land u...

Ibrarul-Haq* and Malik Muhammad Shafi

...lected from the selected sample households using simple random sampling technique with the help of well-structured and self-administered questionnaires. The study has employed multiple regression model to estimate the relationship amongst the variables. The empirical estimation indicates that all variables except household’s size and agriculture land holding have shown positive and significant influence on income from ...

Asim Faraz1*, Bernard Faye2, Cem Tirink3, Ayman Balla Mustafa4, Amal AlKharusi5, Morteza Bitaraf Sani6, Nasir Ali Tauqir7, Muhammad Arslan Akbar8, Muhammad Usman Saleem9, Rana Muhammad Bilal7, Abdul Waheed1, Muhammad Shahid Nabeel10

...spot regions where the disasters of environmental changes hit severely. The future hope for food security is a camel particularly for drought-stricken areas of the country, such as Cholistan, Thal, in Punjab, and Thar Deserts in Sindh. Camels have the ability to adapt to the harshest climatic conditions when kept for milk, meat, wool, and hides production. Camel production can be enhanced by reducing the age at puberty and calving interval. The current study w...

Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5

...entify ABs residues. The samples were collected from Lahore. Raw milk of buffalo and cow collected from the open market have shown 23.5% as the highest positive and UHT milk as the lowest positive 8.5%. Thus, it is an indispensable question to be further explored prospectively. 
 
Novelty Statement | The detection of antibiotic residues including penicillin and o...

Saima Naz1*, Ahmad Manan Mustafa Chatha2, Sajjad Ali2 and Muhammad Irfan Ullah3

...age, learning, sensory disability and memory deficit. Major portion of insects have malformed growth and mortality rate affected by heavy metals, mainly with the exposure of pesticides. On the other hand, pesticides not only alter the behavior, growth and developmental physiology, gut microbiota, but also cause mitochondrial abnormalities. The use of different antibiotics is also posing threat to bees by causing changes in the structure, productivity and physi...

Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5

...ealth, and environmental safety. A survey was conducted through questionnaire distribution among 1000 literate persons who could easily read English i.e. physicians, veterinarians, lawyers, livestock farmers, managers and other literate stakeholders. This survey comprised of a variety of questions related to one-health, use of antibiotics, antibiotics (ABs) residues in milk, meat and yogourt, the role of food safety, organiz...

Uzochukwu Ephraim Emeto, Chukwuemeka Calistus Okolo* and Nwakaego Ernestina Nweze

...cal evaluations are necessary for deploying meaningful intervention strategies. Such investigations are lacking in the region; hence, the occurrence, risk-factors and nature of cestodiasis and nematodiasis of horses at Obollo-Afor southeastern Nigeria were investigated. Horses billed for slaughter were randomly selected (N = 304) for sampling. About 5grams of faeces and 2ml of blood were collected from each horse into labele...

Basanta Kumar Adhikari1*, Deepak Subedi1*, Sumit Jyoti1, Krishna Kaphle2, Chet Narayan Kharel3, Doj Raj Khanal4

...A total of 89 goat serum samples were tested by using a competitive enzyme-linked immunosorbent assay (c-ELISA) for the presence of antibodies against Mycoplasma capricolum capripneumoniae. Out of the total serum sample tested, 3 were seropositive for CCPP giving an overall apparent seroprevalence of 3.37% and true ser...

Ouedraogo Oumar1,2, Tianhoun Denté Fidèle2,4*, Séré Modou3, Kaboré Adama2, Tamboura H. Hamidou2 and Belem Adrien Marie Gaston4

...tchers without any compensation during the same period. The study shows that tuberculosis exists in the cattle population of the urban commune of Koudougou, and that these animals must therefore be properly inspected to protect human health.

...

Bridget Adah1*, Clara Kwanashie2, Haruna Kazeem2 and Samuel Mailafia1

... (EMJH) enrichment and basal medium, and identified using dark field microscopy. A total of two hundred (200) blood samples and two hundred (200) urine samples each were collected from pigs in Kaduna state, Nigeria. A total of 9 (4.5%) of the cultured samples were positive for the Leptospira organisms and % isolation from blood s...

Manish Gautam1*, Pravakar Kattel2 and Krishna Kaphle3

...s was reported which was said to have originated from Zambia in Africa. LSD can be transmitted by both the vector and non-vector routes and is mainly seen in the rainy season. It mainly affects cattle and buffalo manifesting nodules in skin and mucous membrane with a high fever. Since the first report of this disease from the Morang district of Nepal in 2020, the disease has been spreading in various parts of the country. Cattle are comparatively more affected...

Rasha Ali Taha Hamza1, Atef Saad Osheba1, Hassan Mohamed Sobhy2, Sahar Hussein Abdalla Hekal2* 

....03 mg gallic acid/g dry sample for total phenolic and 28.45mg quercetin /g dry sample for total flavonoid whereas the 90% Ethanol Extract of galangal had 46.12 mg gallic acid/g dry sample for total phenolic and 20.08 mg quercetin /g dry sample for total flavonoid. Two types of burger were manufactured, the first was prepared from Brazilian meat while th...
Roy Hendroko Setyobudi1, Erkata Yandri2, Yogo Adi Nugroho3, Mardiana Sri Susanti4
Satriyo Krido Wahono5, Wahyu Widodo1, Lili Zalizar1, Elfi Anis Saati1, Maftuchah Maftuchah1
Manar Fayiz Mousa Atoum6, Muhannad Illayan Massadeh6, Dwi Yono3, Rangga Kala Mahaswa7
Herry Susanto2, Damat Damat1*, Dyah Roeswitawati1, Praptiningsih Gamawati Adinurani8 and 
Susi Mindarti1
...n La Boite, New York. CP samples were picked from the pulper, oven-dried at 80 °C for 15 h, and then grounded using a coffee grinder. Analyses on Mengani’s CCF and La Boite’s comprised Fe content (Inductively Coupled Plasma Optical Emission Spectrometer) as well as five enhancer agents – vitamin C (Iodimetric titration), beta carotene (UV-Vis spectrophotometer), amino acid (amino acid analyzer), and reducing sugars (colorimetric method). ...

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ayesha Latif2, Majid Hamid Bajwa1, Iftikhar Ahmad1, Ali Zohaib1, Naeem Faisal1 and Muhammad Hamza3

...now-ball non probability sampling method was used due to less adoption rate of MTR with a sample size of 240. Among targeted MTR growers 72% had 6-row riding type mechanical transplanters (MT), 18% had 4-row walk after type MT and 10% had 8-row riding type MT. Super Basmati and PK 386 were found more adopted varieties with 47% and 38% share of rice area. An 11-13% improved yield and 16-20% more net returns estimated with MTR...
Syarif Husen1*, Devi Dwi Siskawardani2, Setiawan Deny Rexmardi1, Erny Ishartati1, Muhidin Muhidin1, Jumpen Onthong3 and Ivar Zekker4
...engrowingmediacompositionsandfourreplicatesinamicro-cuttingplanting.The growingmixmediacompositioncontainedhuskcharcoal+cocopeat+bokashi(goat manure) + bokashi(cowmanure), andhuskcharcoal+ cocopeat+bokashi(cow manure) + bokashi(poultrymanure)resulted13.38stemcutting(G0). The beneficial roles of bokashi in increasing organic matters concentration in the soil, and it was able to repair air and groundwater systems that supported plant roots growth and optimum cap...

Eman Alsayed Hammad and Atef Mohamed El-Sagheer

...cacy comparative of Chitosan, Marjoram emulsion oil, Trichoderma asperellum, and Vermicompost as alternative eco-friendly control agents for root-knot nematode M. incognita infected Pomegranate were evaluated under greenhouse and open field conditions. Results indicated the ability of tested strategies to suppress M. incognita after being applied three times. Under protected conditions the pest reduction percentages of M. incognita and increase percentages in ...

Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1 

...trophoresis (SDS-PAGE) assay of the partially purified MH-OMVs revealed approximately ten protein bands ranging from 25 to 104 kDa. Hemagglutination inhibition (HI) assay confirmed that the immune response was significantly increased in both single-dose and booster-dose immunized groups as compared to calves in the control group. Calves in booster-dose group reach its peak geometric mean (GM) log10 HI antibody titer (3.46 &p...

Md. Serajul Islam1,2, Hongxin Wang1,2,*, Amer Ali Mahdi1, Mohamed Ismael Ahamed2, Zaixiang Lou1 and Fu An Wei3

...xidant capacities of the samples increased by thermal treatment at 180°C for 5 min in liver, 10 min in muscle and hard shell, 20 min in skin and bone by ABTS assay; 15 min in skin, 20 min in muscle, liver, hard shell, and bone by DPPH assay, but FRAP value decreased by heating at different time. These results suggested that grass turtle could be used as food additives to improve the fu...

Weidong Huang1,2,3, Xinyue Liang1,2,3, Xiufeng Xie4, Xingmin Wang2, 3 and Xiaosheng Chen1,2,3*

...aplotypes was 17 and the same elytral color patterns of M. sexmaculatus didn’t share the same haplotype. Furthermore, phylogenetic analyses showed all M. sexmaculatus samples formed a single clade, but the identical elytral pattern individuals do not cluster together as no special relationships among different phenotypes individuals. Our systematic analyses illustrated the

Jiang Wu*

...ferent supermarkets. All samples were isolated and identified by conventional methods as Staphylococcus aureus, Salmonella, Escherichia coli and Listeria monocytogenes. Different bacteria were respectively inoculated into LB medium, the OD600 was adjusted to 0.5, and the initial bacterial concentration was adjusted to about 5×108 CFU/mL. T. officinale were dried in a hot air oven, and chemical substances in the dried powder were separated using Soxhlet e...

Dali Wang1,2, Yujing Zhu1,2, Wancai Xia1,2, Mei Zhao1,2,3, Chan Yang1,2 and Dayong Li1,2*

..., a serious geological disaster, are common at the edge of the Tibetan Plateau, and damage the surface vegetation. Like other natural disasters, landslide and debris flows cause a shift in local ecological conditions, which can have profound impacts on the habitat of non-human primates. This study investigated the characteristics of surface vegetation found within the debris field of a landslide and debris flow that occurred...

Saied Belal1, Ashraf Albrakati1*, Khalaf Alsharif2, Saad Al-Shehri2, Mohamed Alblihed3, Malik Almuqati4, Anfal Alsharif5, Alaa Albarakati6 and Anas Al-sharif7

...igh altitude in much the same way as at other altitudes. The effect of residence at high altitude on the prematurity rate needs further investigations.

...

Noora Hassan Hezam Al-Aqmer1*, Zain Aamir2, Muhammad Farooq Hanif3, Soumble Zulfiqar4, Sibgha Zulfiqar1, Mateen Izhar5 and Abdul Rauf Shakoori4*

...ths after completing the same treatment. Informed consent was taken from participants, demographic data was collected, and 5 mL of blood was drawn from each participant and used for DNA extraction, polymerase chain reaction and restriction fragment length polymorphism analysis. After restriction, samples were run on 2% agarose gel followed by visualization under UV light. Data analysis was done using IBM SPSS 24. We found th...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...g substrate-agar plate assay and laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancin...

Man-Seok Shin1, Hyun-Ju Cho2 and Eun-Jae Lee3*

...p guide management of nuisance egret and heron rookeries in urban forests of Daejeon Metropolitan City, Korea. This study examined 126 locations regarded as breeding sites of egrets and herons at the nationwide census conducted by the National Institute of Environmental Research between 2019 and 2020. In addition, 252 randomly selected locations were used to identify the significant variables among a total of 15 environmental variables within 4 factors (topogr...

Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3 

...ingle layer of pregranulosa follicular cells are called primordial follicles and they remain dormant in the meiotic prophase I stage. Activation of primordial follicles initiate through pregranulosa follicular cells. The primordial follicle takes several months to grow and develop to preovulatory follicles in mammals. Changes occur during follicular growth and development stages on the oocyte and the surrounding cumulus cell...

Aiman Al Mufarji, Abd El-Nasser Ahmed Mohammed* 

...mp; 50.0 g daily). Blood samples were collected and were subjected to analysis through hematology and plasma biochemistry analyzers. The hematological parameters include red and white blood cells (RBCs & WBCs), hematocrit (Ht) and hemoglobin (Hb) values. The plasma biochemistry parameters include plasma glucose, proteins, liver and kidney functions and minerals values. The results indicated that organic M. oleifera leaves contain protein (28.28%), carbohyd...

Suhu Duan, Zaiqiang Li, Zhenzhen Fan, Mengran Qin, Xiaoxue Yu* and Liuan Li*

...ct egg quality and monounsaturated fatty acid content. A total of 160 randomly selected healthy Gallus domesticuss, aged 26 weeks, were randomly divided into 4 groups, and each group contained 40 chickens that were fed outdoors in an activity area of 63 m2 with free food and portable water. The CK was fed basic diet, and the experimental group added 1%, 3% and 5% linseed oil to the basic diet (TP1, TP2, TP3), respectively. On the 10th, 25th and 40th day of the...
De Hu, Jia Wang, Huan Wang, Xiang-Yang Leng, Tian-Yi Zhao* and Shu-Min Wang*
..., namely control (normal saline), model (normal saline), ranitidine group (0.03 g/kg) and FP groups (1 and 2 g/kg). Stress gastric ulcer was induced by water-immersion and restraint stress (WIRS) after 10 days of drug administration. To observe the histopathological changes of gastric mucosa. the activities of pepsin, superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) in ...

Muhammad Anwar Ul Haq1*, Tariq Mukhtar1, Muhammad Inam-ul-Haq1 and Azeem Khalid2

... Dilnasheen, Wer and Bemisal showed highly susceptible reaction against M. incognita. All the genotypes showed significant differences in number of galls, eggmasses, females, root and soil populations, reproductive factors and eggmass/gall and eggmass/female ratios. Minimum galls, eggmasses, and females were observed on resistant and moderately resistant genotypes. On the other hand, maximum values in these parameters were recorded on susceptible and highly su...

M.A. Salam1,*, Sarada Kanta Bhagabati1, Rajdeep Datta1, H. Ramananda Singh2 and Gunajit Oinam2

... fish production without sacrificing the lake’s ecosystem with the help of full participation of community.

...

Qiong Xiang1, Jing-Jing Li1, Qian Zhang1,3, Rong-Bo Tian1 and Xian-Hui Li1,2*

...nical hyperalgesia in dorsal root ganglion neurons (DRGs) based on Pinch-nerve injury painful model. However, more details on the role of SSTR2 involved should have been clearly elucidated. Here in this study, we focused on the early phase of painful process; detected the painful hyperalgesia and allodynia induced by Pinch-nerve injury firstly; and then we analysed the expression of SSTR2 protein in DRGs after injecting Carrageenan, the inflammation-induced re...

Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4 

...y was conducted on fecal samples from suspected parvovirus dogs were taken from different provinces in north Iraq for PCR testing, and 76% of such samples were confirmed to be positive. Sequence analysis showed two CPV-2c, six new CPV-2b, and ten new CPV-2a variants. In the CPV-2 studied, several unique and existing mutations have been discovered, indicating the emergence of CPV-2c variation in Iraq.  Genetic differenti...

Serdar Bektas* and Ozer Ayik

...infection with Aeromonas salmonicida, in rainbow trout (Oncorhynchus mykiss) was performed. In order to compare the daily alterations of the hematological parameters and glucose-6-phosphate dehydrogenase activity, blood samples were taken 1,3,7,14 and 21 days after the infection. Total red blood cell values, were significantly decreased in 7 and 21th day, a significant decrease in hemoglobin concentration noted only in the 2...

Ahlam G. Khalifa1*, Walaa A. Moselhy1, Hanaa M. Mohammed2 and Khaled Abdou1 

...deltamethrin loaded chitosan Nps at a dose of 30.44 mg/kg BW. All remedies were administered by oral gavage 5 days a week for one month. The deltamethrin group showed a highly significant elevation in chromosome aberrations. Also, the ratio of polychromatic erythrocytes to normochromatic erythrocytes proved that deltamethrin had a stronger cytotoxic effect on the bone marrow cells than the CS/DM Nps and S/DM Nps groups. It was concluded that these innovative n...

Nkiru E. Ekechukwu, Felicia N. Ekeh, Chinenye M. Ohanu, Hope C. Ezinwa and Ifeanyi Oscar N. Aguzie*

...y, and distribution. The sampling was carried out over a period of eight weeks, using sweep net and hand picking as the collection methods. Overall 12 species were recorded, Orba had the lowest species diversity with 7 species while Obollo-afor and Ikpa recorded 10 and 9 species respectively. Dipteran and Coleoptera were the most diverse and abundant order observed with Musca domestica (Diptera: Muscidae) being the most abundant species encountered. Equitabili...

Seun Eui Kim1,2*, Myoung-Hoon Lee1, Hye Myoung Jang2, Garam Park2, Wan-Taek Im3, Gwang Joo Jeon2,4* 

...ity and cell migration assay were tested and the expression of 17 genes in related signaling pathways was analyzed to determine the potential of CK as an anticancer agent for neuroblastoma. For the study of human neuroblastoma, SK-N-MC cells were studied. IC50 values were 33.06 μM and at 40 μM and 60 μM of CK, the wound coverage rates were 11.5% and 0.5%, indicating the cell migration was strongly inhibited. Among the apoptosis-related genes of BAK1, ...

Tabassum Ara Khanum, Nasir Mehmood* and Shahina Fayyaz

..., Pakistan, several soil sample analysis revealed one new and one known free-living soil nematode species, Neorhabditis andrassyii n. sp., and Poikilolaimus oxycercus which represents a new record of this species from Pakistan. Neorhabditis andrassyii n. sp. is characterized by having male (1109-1222) µm long body, spicules 32-44 µm long, gubernaculum slightly curved, boat shaped with flat distal end eight pairs of bursa

Amreen Zahra1, Mushtaq A. Saleem1*, Hasnain Javed2 and Muhammad Azmat Ullah Khan3

...ficiency virus (HIV), causative of AIDS, mainly leads to reduced immunity provoking increased vulnerability to opportunistic infections such as Hepatitis C virus (HCV). Here, we present co-occurrence of HCV and HIV in male IDUs from four major cities of Central Punjab, Pakistan. Initial screening for HIV and HCV was performed using ELISA and real-time PCR. A high HCV and HIV co-infection w...

Jawad Abdullah Butt1*, Yasmin Nergis1, Ahmad Hussain2 and Mughal Sharif1 

...eters of Thar local coal samples from Block-II and adjacent Block-VI for toxicity consideration and to estimate the potential of Thar coal assets for environment-friendly consumption in power production. In the current study, forty Thar coal samples from each Block II and adjacent Block VI were analyzed for their physicochemical and pyrolysis properties with the chemical configuration of coal ashes. The mean moisture content...

Nour H. Abdel-Hamid1*, Walid Elmonir2, Eman I. M. Beleta1, Rania I. Ismail1, Momtaz Shahein1, Mahmoud E. R. Hamdy1 

...-preferable hosts and passages of Brucella strains are likely to have resulted in such heterogeneity. We concluded that the acceptable diversity induced by ERIC-PCR and RAPD PCR allows sufficient discrimination between Brucella isolates. These findings recommend the application of these techniques as an inexpensive and quick tool for traceability of B. melitensis and B. abortus strains, especially in developing countries with limited resources needed for other...

Hamidur Rahman1*, Asghar Ali Kamboh1, Nazar Ali Korejo2, Atta Muhammad Memon3, Rashid Ali Korejo3, Manzoor Ahmed Chandio1 

...an. A total of 100 serum samples, 50 each from male and female camels were collected from district Sibi, Pakistan and analyzed by competitive enzyme linked immunosorbent assay (cELISA), and rose Bengal plate test (RBPT). The overall seroprevalence of brucellosis in camels was observed as 13% and 17% by RBPT and cELI

Lopamudra Samantaray*, Yashaswi Nayak 

...s are cost-effective and safe natural growth enhancers for broiler chickens with health-promoting properties.

Keywords | Feed intake, Health, Impact, Phytobiotics, Poultry 

...

Zhongqing Wang1, Qiuyue Wu1, Ruiling Ye1, Fazul Nabi1,4, Yangfei Shang1, Sarfaraz Ali4 and Juan Liu1,2,3*

...tylodes macrocephala polysaccharide (AMP) on the quantity of intestinal intraepithelial lymphocytes (iIELs), and the IL-6, TNF-α mRNA transcription level in duodenum of diarrheal mice. A total 72 mice were randomly divided into 6 groups: control (CG), infection (IG), positive drug (PG), AMP low/middle/high dose (LG/ MG/ HG) groups respectively, (n=12). The mice were induced by diarrhea with E. coli (5×107CFU) via i.p injection (0.5 mL/time/d) after...

Abdul Majeed1, Zhenlin Liang2, Lixin Zhu2, Chunli Liu2, Muhsan Ali Kalhoro1,* and Faisal Saeed1

...fy;">Alepes djedaba (Forssakal, 1775), is commonly known as yellowtail fish/shrimp scad, belonging to Carangidae family widely spread in tropical waters. A. djedaba is commercially important fish species from northern Arabian Sea, Pakistani waters. Present study was conducted on stock status of shrimp scad A. djedaba fishery from Balochistan coast, Pakistan. The data was collected from March 2019 to February 2020, and a total of 1,027 pairs of length-weight an...

Mujahid Tanvir1, Muhammad Asam Riaz1*, Muhammad Zeeshan Majeed1, Mazhar Iqbal Zafar2, Muhammad Tariq3 and Muhammad Bilal Tayyab1

...n Valley and surrounding salt range of Pakistan bioassayed against C. quinquefasciatus larvae, eighteen botanicals exhibited more than 50% larval mortality in 48 h exposure. The most effective botanical extracts were Maerua arenaria Forsk, Nerium indicum Mill., Withania coagulans Dunal, Suaeda fruticosa (L.) Delile, Olea ferruginea Wall., Adiantum capillus-veneris L. and Dicliptera bupleur...

Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5

...rom a total of 1250 milk samples from buffaloes were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82....

Mei Zhao1,2, Xiaoqin Zeng1, Wancai Xia1, Baoping Ren3,4, Hao Feng1, Fan Wang1, Qihai Zhou5,6* and Dayong Li1,7*

...lated behaviors via scan sampling and focal animal samples during the birth of two infant Yunnan snub-nosed monkeys (Rhinopithecus bieti) in 2017. One of the infants was born during diurnal to a multiparous female, while the other one was a nocturnal birth to a primiparous female. Actual parturition lasted only 7 min for diurnal birth. During the parturition process, the mother received what could be described as assistance ...

Nausheen Irshad1, Maria Akhter1, Tariq Mahmood2*, Faraz Akrim3, Muhammad Rafique Khan1 and Muhammad Sajid Nadeem4

...d” mammal, in Bunjosa Game Reserve, Azad Jammu and Kashmir, Pakistan. The species occurrence was confirmed by direct field sightings (live and kill), and indirect signs (pugmarks, burrows, fecal droppings, and reported sightings by local community). During one-year study period (September 2015-August 2016), a total of 28 individuals of the species were sighted in the field including 18 live and 10 dead specimens. In addition, 93 fecal

Rabia Goher, Inamullah and Mohammad Akmal*

...ng genotype may not necessarily reflect its potential performance when subjected to a different Agro-Environment (AE). This study, therefore, aimed to assess the foremost developmental stages of the selected genotypes of wheat in three AE i.e. AEP (Peshawar), AEK (Kashmir) and AEC (Chitral). An experiment was conducted in a randomized complete block, having 3 replications. Four cultivars (i.e. Pirsabak-2005, Pakhtunkhwa-2015...

Samreen Khan*, Salma Javed and Nasira Kazi

... was retrieved from soil sample collected around roots of paper flower (Bougainvillea spectabilis L.), thoroughly described and illustrated from village Sarai Naurang located in southern district Lakki Marwat, Khyber Pakhtunkhwa, Pakistan. The population was identified as new species and related with Group 2 of Aphelenchoides species, distinguished via length of female’s body 428-444 µm (0.42-0.44 mm), cephalic region rounded and offset. Lateral fi...

Ikele*, Chika Bright, Ujunwa Aghaji, Mgbenka, Benard Obialo 

...tems. The CN ratios (cassava and wheat) of 10:1 and 20:1 were; cassava (CA 10:1, 20:1) groups C, D, and wheat flour (WH10:1 and 20:1) groups E and F, respectively. Meanwhile, the normal controls, group A (inoculum, no carbon addition) and B (no inoculum, no carbon) had 50% water exchange and received only fish feed at 3% body weight. The physicochemical parameters maintained the tolerable limit for catfish farming. Signific...

Eman Alsayed Hammad1* and Mahmoud Mohamed Hassanin Hasanin2

...loidogyne javanica and Fusarium oxysporum on coleus plants, Coleus forskohlii in vitro and greenhouse conditions. Nanoemulsions of thyme (droplets size was in the range of 25.4–32.9 nm) and spearmint (droplets size was in the range of 5.91–9.77 nm) at concentrations 4000 and 5000ppm separately, recorded the best results in vitro investigations, completely prevented F. oxysporum growth at all concentrations, and increased M. javanica mortality by 10...

Phassakon Nuntapanich1*, Hathaichanok Nuntapanich2 and Woraman Maicharoen3

...ts of fish from the wholesale farmer. All of these farming practices are efficient using of natural resources as the local wisdom that shield against inevitable shock in household economy and food security including encourage interaction between people in the community. The farmers in the study site obtain benefits from ecosystem services such as increasing income and maintaining food security (rice and fish) by using local wisdom. Therefore, to ensure sustain...

Muhammad Ismail1,*, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1 and Umair Abbas1 and Riaz Hussain2

... justify;">Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the economic pests of horticultural crops. This invasive species causes substantial economic loss to citrus produce each year in Pakistan. Farmers rely on persistent synthetic insecticides for fruit fly control. Insecticidal phytoextracts are biorational alternates to hazardous synthetic insecticides. This study evaluated the efficacy of aqueous extracts of neem (Azadirachta indica A. Jus...

Md. Enayet Kabir1, Md. Jahangir Alam1*, Md. Mosharaf Hossain2, Zannatul Ferdaushi3 

... fed TMR (TMR2) with the same feed ingredients in the same quantity except probiotic fermented rice straw instead of untreated rice straw of TMR1. The trial lasted ninety days. Feeding of TMR2 improved the growth rate (P<0.05) of fattening cattle compared to others. Furthermore, TMR2 fed cattle had higher (P<0.05) values for hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs) and lactobacillus count. Seru...

Tariq Mahmood1*, Shakeela Ismail1, Faraz Akrim2, Muhammad Farooq1, Nadeem Munawar1 and Muhammad Raza Khan1

...ition of grey wolf in Deosai National Park, Pakistan to record how much proportion of its diet comprises of livestock, leading to human-wolf conflict. Field surveys were made on monthly basis from July 2018 to December 2019 for data collection. The distribution of the species was determined by surveying the potential habitats and recording its direct and indirect field signs (such as scats, pug marks, prey remains, hairs), while diet composition was investigat...

Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*

...e anesthetized and blood samples were collected to scrutinize the various parameters such as creatine kinase myocardial band (CK-MB), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine, troponin-T (TRT), cholesterol, C - reactive protein (CRP), and concentration of mitochondrial enzymes viz., Ca2+, Na+ and K+ ions were estimated in blood. Heart tissue was also isolated for caspase-3 activity. Our result showed that naringenin pretreatment si...

Zahra Jamilah Sabrina1, Adrian Pearl Gunawan2, Beryl Reinaldo Chandra2, Ilham Pangestu Harwoko3, Johannes Marulitua Nainggolan4,Widi Nugroho1* 

...acture causes physical disability, psychological stress or even fatal to a patient. Autograft and allograft have long been used to improve fracture healing, but flaws limit their use. Xenograft from micro-carbonated hydroxyapatite (micro-CHA) and nano-CHA from bovine bone combined with Platelet-Rich Plasma (PRP) was hypothesized to accelerate fracture healing. This study aimed to characterise micro-CHA and nano-CHA synthesized from bovine bone and analyze thei...

Weam Mohamed Baher1, Wageh Sobhy Darwish2*, Abdelazim Elsayed Elhelaly3,4 

...he prevalence rate of Anisakis larvae in two marine water fish commonly consumed in Egypt, namely, herrings and sardine. Samples were collected equally from Suez, Ismailia, Damietta, Port Said and Alexandria. The obtained results revealed overall incidence rates of Anisakis larvae in herrings and sardine at 70% and 50%, respectively. The parasite infeste...
Mudassar Jehan1, Masroor Ahmed Bajwa2, Mohammad Masood Tariq2, Asim Faraz3*, Abdul Samaad2, Jameel Ahmad1 and Yousaf Hassan Barozai2
...ls of Balochi breed were sampled for DNA extraction. Some specific markers (15 out of available 30 SSR markers) were employed in the present study to highlight genetic polymorphism. The PCR was utilized for amplification of individual DNA samples. All of the 15 SSR markers were amplified. After gel documentation, a total number of 97 alleles were recognized having 1 to 5 polymorphic forms (OARFCB193, OARJMP29, MAF33) to 4 (O...

Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1

...f EDTA in the activity assay mixture reduced the β-lactamase activity to 91% while Zn2+, Co2+, Mn2+ enhanced the enzymatic activity to 144, 121 and 108% when used at a final concentration of 1 mM. The ionic and non-ionic detergents showed slight inhibitory impact on the recombinant β-lactamase activity. The enzyme exhibited the Km and Vmax values of 2.27 mM and 45.45µmol/min, respectively when benzylpenicillin was utilized as substrate. The deg...

Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1* 

...-Suef, Giza, Minia). All samples were cultivated on PPLO media and detected under stereo-microscope for fried egg colonies. Forty-three samples were detected as Mycoplasma spp., using PCR for species identification, MG, MS, and Untyped strains, with a high prevalence of MG (27.9%) and lowest for MS (8.8%). An rRT-PCR used for detection of AIV-H9N2 and IBV showed high prevalence for IBV (88.2%) and AIV-H9N2 (52.9%), the hig...

Samira Merabet1, Nora Khammes-El Homsi1,*, Lydia Aftisse1 and Stéphane Aulagnier2

...ed murine species to the same physical and ecological conditions in a locality of Great Kabylia (Algeria). Apodemus sylvaticus, and Mus spretus synchronized their reproduction in winter and spring, Lemniscomys barbarus in spring and summer. Photoperiod and temperature cannot explain this reproduction shift that might be linked to the feeding ecology of the species.

...

Weam Mohamed Baher*, Gamilat A. El said 

...d results indicated an unsatisfactory hygienic status of the retailed chicken meat in the study area, in terms of high microbial counts. Chicken breast collected from rural areas had significantly (p< 0.05) the highest counts. A significant reduction of the microbial load of chicken breast was achieved after treatment with nisin, and gingerol, particularly at 2%. For instances, TBC was significantly (p< 0.05) reduced by 26.98%, 31.94%, 32.84%, and 38.83%...

I Gusti Lanang Oka Cakra1*, Anak Agung Ngurah Badung Sarmuda Dinata2,  I Gede Mahardika1,  I Gusti  Nyoman Gde Bidura1

...ical plant that contains saponin that may be used as de-faunating agent in ruminants. In some areas, these plants commonly being used as an alternative feed for ruminant, particularly during the dry season, but the study of its effect was limited. This study aims to determine the effect of additional Hibiscus leaf flour (HLF) in the concentrate on protein balance, blood metabolic profile, and body composition of Etawah crossbreed goats. A total of 20 goats wit...
Tri Eko Susilorini1*, Ahmad Furqon2, Wike Andre Septian1, Desinta Wulandari3, Suyadi Suyadi3
...y 1 to 4. Blood and milk samples were collected in Senduro district, Lumajang. The traits observed were milk yield, fat, lactose, solid non-fat, total-solid, and protein. The CSN3|Alw44I polymorphism was analysed using Polymerase Chain Reaction-Restricted Fragment Length Polymorphism method. The result showed that three genotypes (CC, CT, and TT) and two alleles (C and T) were found. The frequencies of CC, CT, and TT genotypes were 0.450, 0.500, and 0.050 resp...
Paulus Klau Tahuk*, Oktovianus R. Nahak T.B., Gerson F. Bira, Adrianus Manek, Donatus Manek, Tobias Y. Purnama, Emanuel Bubun, Bergias Subay
...take were relatively the same among treatments. However, the intake of CP and CF in T2 and T3 was higher (P<0.05) than T1. On the other hand, the TDN intake of T2 was higher (P<0.05) than T1. However, T3 has relatively the same consumption with T2 and T1. Furthermore, the nutrient digestibility was relatively the same among the treatments, while the daily body weight gain (ADG) of T2...

Aiman Batool1, Muhammad Sohail Sajid1,2*, Hafiz Muhammad Rizwan3**, Asif Iqbal4, Imaad Rashid5, Ibadullah Jan6, Faiza Bano7, Fiaz Ahmad8, Waqas Ahmad1, Muhammad Nisar Khan1 

...kistan. Faecal and blood samples were collected along with ectoparasites directly from the animal rectum, jugular vein and skin, respectively. The examination of gastrointestinal (GI) parasites and haemoparasites was carried out through qualitative and quantitative methods, and Giemsa-stained thin smear, respectively. Morphological identification of ectoparasites was carried out under the stereomicroscope. The overall preval...

Hye-myoung, Jang 1,3, Ju-Hyeun, Kim3, Garam Park1, Yoon Dong Choi4, Sun-Eui Kim1,5, Gwang Joo Jeon1,2* 

...pression. Western blot assay was made for Aβ and τ-protein expression. For blood chemical compositions, both LDL and HDL tended to increase in AE group than those in the control. Though body weights increased in the AE group than those in the control, AE significantly reduced both Aβ and τ-protein formation at p=0.05, indicating potentially preventative and medicinal effects of Agarwood against Alzheimer’s and other dementia related dis...
Manatsanun Nopparatmaitree1*, Pornpan Saenphoom1, Sittichai Bunlue1, Silchai Washiraomornlert1, Warangkana Kitpipit2,3,4, Soranot Chotnipat1
...ased on a corn-soybean basal diet and a control diet supplemented with 10, 30, and 50 g/kg TABP combined with 2 g/kg probiotics. This study shows that TABP combined with probiotic supplementation significantly affected the apparent digestibility of dry matter, ether extract, crude fiber, and crude protein (p<0.05). The supplementation of TABP combined with probiotics increased the lactic acid bacteria, Enterococcus sp., and volatile fatty acids (p < 0.05...

Muhamad Khairul Riza, Valentinus Priyo Bintoro, Sri Kismiyati, Sugiharto Sugiharto*

...s determination and meat sample was collected at day 37. CONT meat had lower (P < 0.05) pH than other meats. HSDAT meats had greater (P < 0.05) redness (a*), whereas yellowness (b*) values were lower in CONT than other meats. CONT meat had higher (P < 0.05) unsaturated and saturated fatty acids (FA) than other meats. CONT had higher (P < 0.05) n-3 to n-6 FA ratio than HSDT, but...
Mohammad Belal Shaker1, Waleed Rizk El-Ghareeb2,3*, Marwa Magdy Seliem4, Wageh Sobhy Darwish3, Bassam Abdulla Alhawas2, Ahmed E. Tharwat3
... source of omega-3 polyunsaturated fatty acids, high biological value protein, minerals, and vitamins. However, crustaceans are regarded as potential sources for the transmission of foodborne pathogens. Aeromonas spp. is one of the opportunistic microorganisms, that normally inhabits aquatic environments such as fresh, and marine water bodies. Aeromonas spp. might cause food-borne gastroenteritis that might be complicated to cause septicemia, meningitis, endoc...

Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*

...addition of organic acid salts (OAS’s) significantly (P<0.05) enhanced weight gain and feed efficiency. Dietary supplementation with OAS’s enhanced significantly (P<0.01) the weight of carcass and dressing percentages of birds. The liver, heart, gizzard, and spleen were unchanged (% of body weight). Birds fed diets enriched with SF or SP had considerably (P<0.05) longer and wider guts than the control group, and scored zero in intestinal l...

Chandni Wajid1, Abdul Hameed Baloch1, Ahmed Nawaz Khosa1*, Hubdar Ali Kaleri2, Nasrullah Bangulzai1, Sarfraz Ali Fazlani1, Haleema Sadia3, Saeeda Kalsoom4, Muhammad Bilawal Arain2, Wassem Ali Vistro2 

...gene analysis, the blood sample were collected from the selected sheep breeds and processed for DNA extraction. Primers were designed using Primer 3 software and PCR was performed. PCR products were sequenced and analyzed for observing genetic variation in PROP1 gene of these four sheep breeds of Balochistan. The result for PROP1 gene analysis showed 7 variations including 3 frame shift and 4-point mutations with c.63G>A, c.83G>A, c.94G>T, c.104C>G...
Emtenan M. Hanafi1*, Fouad M. Tawfeek2, Walid S. El Nattat1, Moetazza M. Alshafei3, Seham S. Kassem3, Kawkab A. Ahmed4, Enas N. Danial5, Hagar Elbakry3, Hoda S. El-Sayed2
...r 2 months, animals were sacrificed. Results showed that SP had antimicrobial effect against pathogenic bacteria while, it kept probiotic bacteria alive. The animals fed on HFD showed elevated lipid profile, leptin, oxidative markers and low level of testosterone and the epididymal Spermatozoa had low sperm viability and higher abnormalities than control group. Feeding the microcapsules (G2,G3) improved the animals’ health condition. Animals in G2 showed...
Noha M El-Motaily1, Ossama M Abdou1, Heba S Farag1, Kawkab A Ahmed2, Mahmoud Saber1*
...of the normal skin commensal Demodex mites. Hemato-biochemical changes were previously reported in patient dogs with demodicosis due to the effect of stress caused by mites. This study was conducted on 38 dogs, 20 of them were used as control group and 18 were demodicosis infected dogs, for evaluation of hematological, biochemical and histopathological alterations. Beside detection of the antioxidant trace elements (Zn, Cu, Se) levels in dogs suffering from de...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
...reas in the lung. Pooled samples from the investigated quails were inoculated in specific pathogen free embryonated chicken eggs (SPF-ECEs). The dead embryos showed hemorrhages, congestion and dwarfism. The collected allantoic fluids were examined later by haemagglutination (HA) and haemagglutination inhibition (HAI) test. The allantoic fluids of 5 pooled samples expressed positive using specific Newcastle disease virus (NDV...

Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4 

...he presence of steroidal saponins. Therefore, the main objective of this study was to determine the effects of different levels of B. decumbens diets on the in vitro gas production and ruminal fermentation characteristics. Graded levels of B. decumbens were mixed with P. purpureum, where 10% was identified as the low-level B. decumbens diet (T2) and, 60% was identified as the high-level B. decumbens diet (T3) based on the concentration of

Asaad A. Fares1*, Mohamed M. Ghanem2, Yasein M. Abd El-Raof2, Hossam M. El-Attar2, Ameer A. Megahed2, Heba M. El-Khaiat2

...ood and mid-stream urine samples were collected from 24 cows fed on DCAD ration and 24 cows fed on non DCAD ration daily starting 24h before calving (-1day), the day of parturition (0day), one day after parturition (+1), and second day after parturition (+ 2). Feeding DCAD ration lead to decreased urine pH and urine creatinine, but increase Ca urine execration in the DCAD group. However, the DCAD group showed a significant increase (P<0.05) in Ca, Mg, P, K,...

Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby* 

...s, 200 different chicken samples were examined for isolation of E. coli and for colistin resistance gene. Eighty-three selected samples (chicken breast) were subjected to detection of colistin residues by HPLC (High-performance liquid chromatography). In this research, we used polymerase chain reaction (PCR) for the detection of the mcr-1 gene, and phylogeny was performed on three isolates to detect mcr-1 gene mutations and ...

Ida Bagus Oka Winaya1*, Anak Agung Ayu Mirah Adi1, Putu Henrywaesa Sudipa2, I Gusti Ketut Suarjana2

... of streptococcosis. The samples were 35 cadaver of post weaning piglets that showed muscle spasm and swollen joint at several villages in Badung Regency, Indonesia. Samples of the brain and synovial tissue were processed using hematoxylin-eosin dyes (HE). On histopathological examination, congestion, edema, mild infiltration of neutrophils and macrophages in the meningen, and ischemic neuronal pyknosis were found in the brain. In the synovial tissue, there is...
Neeranoot Detcharoenyos1, Nakrob Pattanapon1, Soontaree Petchdee2*
...er dog presented to Kasetsart Veterinary Teaching Hospital, Kamphaeng Saen, with progressive paresis over two weeks. The dog was ultimately diagnosed with a spinal tumor by radiography and MRI. In this study, the dog was successfully treated by dorsal laminectomy of T8-T9 to excise an abnormal spinal mass, and a modified pedicle screw-rod was fixed to T7-T10 for stabilization. The biopsy result was cartilaginous exostosis (o...

Tu Thi Minh Tran1*, Diep Hoang Tran1, Thuong Thi Nguyen2, Tomas J. Acosta3, Takeshi Tsuruta4, Naoki Nishino4, Hai Thanh Duong5

...fecal, milk, and uterine samples from dairy cows and two sets of airborne dust and water samples from the cowshed were collected from two farms in Ho Chi Minh City at 20–40 days postpartum. The microbiota was characterized using Illumina sequencing of the V4 hypervariable region of the 16S rRNA genes. The predominant species in the fecal microbiota was not as predominant in the milk microbiota, but occasionally appeare...
Rubaijaniza Abigaba1,4*, Pharaoh C. Sianangama1, Progress H. Nyanga2, Edwell S. Mwaanga3, Wilson N.M. Mwenya1
...employed to obtain sex-disaggregated data from 622 respondents using a semi-structured questionnaire. Descriptive statistics including frequencies, mean, and standard error of the mean as well as inferential statistics, namely, Chi-square, Mann-Whitney U test, independent samples t-test, ANOVA, and the post-hoc tests were used to analyse the data. Out of 622 respondents, 66.9% were females while 33.1% were males. This study ...

Aamir Ibrahim1, Bingyao Chen1, Hassan Ali2, Imran Ali3, Yang Cao1 and Guang Yang1*

...the Indus River from Taunsa barrage (District Muzaffargarh, Tehsil Kott Addu) to just downstream of Ghazi Ghat near Samina (District Dera Ghazi Khan) in Punjab, Pakistan. We successfully photographed and first reported seven types of skin marks originated from their natural or social interactions and anthropogenic activities. Dead bodies of five calves were collected from two different subpopulations in the Punjab river section, two from the Chashma-Taun

Zahra Hussain, Zulfiqar Ali* and Rida Ahmad

...ong infectious diseases, salmonellosis, infectious coryza and colibacillosis were most abundant with 34, 34 and 29 cases respectively. Out of 99 different diseases and pathological conditions in birds, maximum variety was recorded in ring-necked pheasant (Phasinaus colchicus), black-shouldered peafowl (Pavo cristatus) and green pheasant (Phasianus versicolor). Comparison of different study...

Ghulam Abbas1*, Muhammad Arshad1, Muhammad Saeed2*; Safdar Imran3, Ashgar Ali Kamboh4, Duraid KA Al-Taey5, Muhammad Asad Aslam1, Muhammad Saeed Imran6, Muhammad Ashraf3, Muhammad Asif7, Abdul Jabbar Tanveer8, Razia Abdul Majid Qureshi9, Maria Arshad1, Hussain Ahmed Khan Niazi1, Muhammad Tariq10, Sikandar Abbas1 

...greatly outweigh their disadvantages like decreased palatability. Organic acids can increase egg productivity and enhance the egg quality in layers. In broiler, use of OA is associated with improved weight of birds and feed conversion ratio. Dietary OA showed 1.85-8.48% increase in the FCR of chicken. Lactic acid fed 0.3 g/kg diet reduced Escherichia coli and Salmonella significantly in the cecum of broilers. Butyric acid fed 500 g/t feed significantly increas...

Samia Azad1, Iram Liaqat1*, Riffat Iqbal1 and Uzma Rafi2

...re. Shrinkage of sub mucosal tissue, enlarged flattened villi, increased apoptosis and degenerated nuclei alongwith missing cytoplasmic boundaries were observed at high concentration of exposure (0.4-1.0 ppm). Likewise, CdCl2 at high concentrations (0.6-1.0 ppm) had toxic effects kidneys and severe pyknosis, degenerated renal tubule, loss of cell integrity in complete tissue were observed. Pronounced degeneration of liver tissues, severe vacuolization, remarka...

Ghulam Murtaza1,6*, Muhammad Irfan Fareed2, Noshaba Munawar3, Muhammad Nadeem Khan4, Gul-E- Zahra5, Taskeen Ahmad6, Muhammad Daud6 and Taqi Raza6

...erent insecticides (Spinosad 100%EC, chlorfenapyr 36%SC, pyriproxyfen 10% EC, matrine 0.5 AS and imidacloprid 70% WDG) against Aedes aegypti larval mortality under laboratory conditions. Result indicated that Spinosad (77.32% mortality) and pyriproxyfen (66.28% mortality) are more toxic to A. aegypti larvae as compared to matrine, chlorfenapyr and imidacloprid after 72-h exposure. While minimum mortality was recorded after 2...

Iram Liaqat1*, Umaima Bibi2, Muhammad Arshad3 and Najma Arshad2*

...resently, there is universal interest to discover new and safe antioxidant compounds from natural sources, to reduce oxidative injury to the cells and overcome oxidative deterioration of foods affecting human life. The study was designed to assess the antioxidant capability of six selected medically principal plants; Hyssopus officinalis, Origanum vulgare, Thymus vulgaris, Glycyrrhiza glabra, Cordia latifolia and Zizyphus ju...

Junaid Ali1, Muhammad Nawaz2, Muhammad Ilyas3, Muhammad Umer Chattha1, Imran Khan1, Muhammad Bilal Chattha4*, Muhammad Arshad5, Muhammad Akram6, Mina Kharal7, Ehsan Ullah1, Muhammad Talha Aslam1, Fareeha Athar1, Ayesha Mustafa1 and Muhammad Umair Hassan1

...mi-arid conditions of Faisalabad.

...

Abdul Majid1*, Farah Naz1, Nasrullah Laghari1, Sanaullah Abbasi1, Sham Lal2 and Safdar Ujjan3

...ucture was maintained in saline phosphate buffer (PBS) pH (6.4, 7.4 and 8.0), similar to that of water. Temperature based experiments depicted the resistance due to changes in the secondary structural conformation up to 50 °C, however such resistance was reversible after the cooling to 20 °C. In both solvents (PBS and water) lysozyme was predominantly folded to α-helical conformation. At 50 °C, helices were altered to turns. However, in prese...

Nur Prabewi , Supriyanto, Budi Purwo Widiarso* 

...bsp;meat has some disadvantages such as antibiotic residues, low antioxidant and tenderness value, and protein instability upon heating, thus it needs special treatment. Marination is a flavored liquid that serves as the ingredients of the meat marinated, and is usually used to improve the yield and shelf-life of meat. The research aims to study the effect of herbal marination on the quality character...

Asim Zubair1*, Iffat Batool2 and Ghulam Akbar Malik3

...ced to take out loans to satisfy their basic needs, and the requirement of collateral places them at the mercy of the black market. Mostly people in that rural areas were depending a lot on NGOs for their basics needs. So, the Purpose of this research was to identify the possible role of NGOs in Pakistan’s Agriculture sector. The experience of non-Governmental and community-based organizations were reviewed in this research. Pakistan occupies a unique po...

Muhammad Musa1*, Saba Saeed2, Azher Mustafa2, Muhammad Ussama Yasin2, Saima Naseer2 and Iftikhar Haider3

...defense activators i.e., salicylic acid, citric acid, benzoic acid, KH2PO4 and K2HPO4 were tested against potato leaf roll virus (PLRV) in field conditions while two nutrient solutions i.e., macronutrients (N, P, K) and micronutrients (B, Zn, Cu, Mn, and Fe) were tested against the disease under glass house conditions during the years 2018 and 2019. All plant defense activators and nutrients were sprayed thrice using three different concentrations (0.25%, 0.50...

Muhammad Zeeshan Nadeem1, Muhammad Farrukh Saleem1*, Muhammad Ashfaq Wahid1 and Muhammad Anwar ul Haq2

...ersity of Agriculture Faisalabad, Pakistan. Three seed priming treatments viz. hydro priming (48h), ascorbate (40 mg L-1) and calcium chloride (100 mg L-1) including one control (no-priming) were tested with four pearl millet cultivars (YBS-98, 18-BY, Ghana White and Sargodha Bajra 2011) during the experimentation. Treatments were arranged in randomized complete block design and replicated thrice. The results revealed that seed germination, plant growth, fodde...

Noor Muhammad Khan1,2, Tariq Mahmood Khalil1,3*, Rashid Rehan2 and Iftikhar Zeb4

...site as the biomass has usage in the local market.

...

Abid Hussain1, Mubbashira Nazir2*, Saira Batool2 and Sidra Majeed1

...t was collected from 110 sample farmers in the year 2018. It is found that adopters of the gypsum technology are more educated, having large family size, operational land and livestock holdings. Gypsum application at a rate of 0.26 ton per acre doubled the productivity (26.80 mounds/ 40kg) of groundnut crop than without its use. Benefit-cost ratios (BCRs) for groundnut crop with and without gypsum application are 4.47 and 3.23, respectively. Similarly, product...

Mehwish Saleem Khan* and Sumama Farooq

...’s why majority of samples (33/60) were obtained from females as compared to males (27/60). From these collected samples exceeding numbers were of blood samples (20/60) followed by urine samples (18/60), pus samples (12/60) and (10/60) samples were of sputum. Bacterial isolates...

Samina Malik1, Tahira Jabeen Ursani1, Naheed Mehmood Soomro1, Naeem Tarique Narejo2, Jawaid Ahmed Khokhar1* and Asif Soomro1

...Circadian rhythm is necessary for the adaptation in the environment. In present research diurnal behavior was more frequently found as compared with nocturnal.

...

Zunaira Fatima Syeda1*, Ahafaque Ahmad Shah2, Farrah Deeba3 and Farheen Malik1

...nveniently selected as a sample of the study. Mean median, mode and range were calculated to describe the data. The difference between the levels of competence was measured with the help of independent sample t-test. The same was applied on gender and group to observe the difference. The relationship between generic competence and students’ grades was found with the help of Pearson c...

Akbar Hayat1*, Marayam Nasir2, Ehsan-Ul-Haque1, Muhammad Asim1, Sohaib Afzaal1, Shagufta Naz3, Muhammad Nawaz1, Abid Mahmood4, Faheem Khadija1, Fiaz Hussain1 and Tariq Mehmood5

Marwa Ragab Saeed Abdallah, Hussein Mohamed Hussein Mohamed, Mohamed Mohamed Talaat Emara, Mai Atef Mohamed

...W, and untreated control samples, respectively. Therefore, the application of WBI/BW edible coating before packaging of pastirma may be safely used at a commercial scale to extend the durability of pastirma.

Keywords | Pastirma, Whey protein, Beeswax, DPPH, carbonyl compound. 

...

Naseem Sharif1,2*, Imran Muhammad Siqqique2, Muhammad Kashif Raza3, Urwa Irshad1, Muhammad Ikhlaq Khan4, Ammara Noreen4 , Muhammad Ahsan Qureshi1, Mohsin Abbas5, Muhammad Maaz Aziz5, Sitwat Riaz5, Komal Aslam5 and Naseem Akhtar6

...ne is highly favoured to save rich genetic resources of this unique jujube specie.

...

Iftikhar Alam1*, Muhammad Ali2 and Muhammad Farooq3*

...cha Khan University, Charsadda, KPK, Pakistan. Data on food and physical activity were collected using a set of food and lifestyle photos, which were intended to describe various situations of on-campus food and active lifestyle environment. Students identified support and barriers for healthy eating. They also identified barriers and support for active lifestyle on campus. The support and barriers identified by students coded under themes of food and active l...

Juweria Abid1, Hooria Latif2, Amir Sohail2, Saroosh Fatima Tahir Syed2, Shakeel Ahmad3, Tooba Asif4 and Muhammad Farooq5*

...reme malnourishment. The sample consisted of 450 university students aged 20 to 25 years. It was observed that majority of the students were day-scholars (59.8%) due to which their daily eating routine was normal i.e. three meals per day and BMI ranges were found to be normal (18.5 to 25 kg/m2) in most of subjects which show no prominent presence of any eating disorder. But some minor eating abnormalities were detected in their eating behaviors. The tradition ...

Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy 

...o 24 month of age. Blood samples were taken from 66 male buffalo calves at 1, 3, 6, 12, 18 and 24 month of age (11 animals at every age). TES, leptin and PRL levels were determined in blood plasma. Results showed insignificant increase in TES and PRL by age advancing at 1-3 month, and in leptin at 1-6 month of age. All hormones markedly increased up to 24 month of age. TES increased by increasing body weight (BW) from 40 to 420 kg. Leptin and PRL increased by ...

Nawal Abd-elhafez Hassanain1, Raafat Mohamed Shaapan1*, Ahmed Maher1, Ahmed Badwi Yousif2, Gomaa Desoky Emam2 

... The collected 504 blood samples were screened for malaria (card test ‘RDTs and microscopic blood film examination), typhoid fever (Widal test, blood culture and stool analysis) and brucellosis (Rose Bengal test ‘RBT’ and also standard agglutination test ‘SAT’) a similar time. The results showed that 29 of 504 examined cases (5.75%) were RDTs positive while al...

Syeda Rubab Zaidi* and Safdar Ali Mirza

...the enzyme, the purified samples of enzyme was significantly greater protein concentration than crude sample so, the optimum pH was 5.0 for laccase activity and the enzymatic extract was thermostable up to 60oC for 1 hour with guaiacol as substrate. The biological production of enzyme have an ecofriendly impact in current scenario. 
 
Novelty Statement | The study is novel in suggesting ecof...

Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7

...y bred she camels. Blood samples from G1 was collected at first day of trial, while G2 animals blood collection was done at day 14th and 21st day post mating. All the blood samples were centrifuged at 5000 rpm for five min; serum was collected and stored at – 18°C for laboratory analysis. Commercially available ELISA kits were used to measure s...

Ahmad Ali1, Zaib Ullah2, Muhammad Javaid Asad1*, Muhammad Rafi3, Muhammad Maqsood4 and Sajid Mahmood5,6

...therapy (PDT) on Hela versatile feasibility underneath outstanding oxygen circumstances becomes quantified by the utilization of the equivalent vintage check. Photodynamic therapy is developing as a potential solution for masses malignancies. The purpose of this task changed into to find the reasonable total of acknowledgment of photosensitizer as medication and portion of laser moderate which may likewise also in addition additionally reason most extreme harm...

Heba Abdo Basha1, Walaa Slouma Hamouda Abd El Naby2*, Samya Erian Ibrahim3, Abeer Fikry Elnahas2 

...d low body weight. Blood samples were collected from each bird for DNA extraction. Four birds were selected randomly/group to be slaughtered for carcass traits. In MSTN, only six non-synonymous SNPs were recorded leading to four amino acid substitution in exon 1 and 2 amino acids substitution in exon 2. In low body weight, only one non-synonymous mutation was recorded at exon 2. The 3’UTR-IGF-2 sequence showed 14 SNPs in high body weight and three SNPs i...

Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2** 

...6. Five hundred (n= 500) samples were collected from fifty farms (5 tracheal and 5 cloacal pools from each farm) have been examined by real time RT-PCR for the prevalence of AIV H9. The results revealed that ten farms (20%) were positive for AIV (H9N2). Three isolates with high CT values have been selected for complete sequencing of hemagglutinin gene. Amino acid sequences showed that these isolates were identical to the Asian strain “A Quail-HongKong-G...

Mohamed A. Hussein1, Bahgat A. Abd El-Rehman2, Kareem A. Eldin2, Mohamed Fouad Mansour1* 

...une response. It must be safe, inexpensive and easy to prepare. This study aimed to develop a new RVF virus vaccine with chitosan nanoparticles and aluminum phosphate nanoparticles as adjuvants and evaluate their impact on biochemical, cellular and humoral immune response in sheep, The prepared vaccines were sterile, safe, emulsion is stable and validity within acceptable limit 0.02 EDF50/...
Faiza Saleem1, Aly Khan2*, Rafia Rehana Ghazi3, Nasira Khatoon1, Samina Waheed1, Mian Sayed Khan4
...und were alive. Five thousand worms in just 4 fish can be very harmful and may lead to further decline in the population of host fish.

...

Oluwatoyin Adenike Fabiyi

...raging a sustainable and safe environment in tomato cultivation.

...

Rizwana Abdul Ghaffar* and Kamil Nadeem

...ng themselves to the mucosal layers. This causes severe pathogenicity in the intestine as a result the pigeon shows signs and symptoms of illness. Pathological studies of tissues from infected intestine reveal several changes from the normal architectural organization of tissues. The most common findings were massive congestion, cellular infiltration, atrophy, hypertrophy and dystrophy of cells and disintegration of cellular layers, fibrosis, necrosis and dila...

Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2

...i.e., on day 34, intra-nasal administration of E. coli of strain χ7122 (serotype 078: K80:H9) at pathogenicity amount (9.4 x 105 cfu E. coli per bird) was done and morbidity and mortality rate was also recorded. The significantly minimum ammonia (ppm) was recorded in CRH (30.53±0.77); however, the maximum ammonia (ppm) was recorded in RH (49.78±0.42) with that compared to SD (33.42±0.25) and RS (43.22±0.56). Similarly, the morta...

Arfan Ahmed Gilal1*, Lubna Bashir Rajput1, Muhammad Ibrahim Kubar1, Ghulam Murtaza Kaleri2, Tanzeela-ul-Zahra1,3, Muhammad Ishaque Mastoi4 and Zeeshan Rasheed1

... features, which is necessary for better management. Accordingly, life table and fertility schedule studies of FAW were conducted under laboratory conditions on maize leaves and stems. Three cohorts comprised of 131, 112 and 105 eggs of similar age were used for both leaves and stems. The results indicated that maximum mortality of FAW in both maize stems and leaves were recorded in pupa and 1st larval instar, respectively whereas, maximum survival was recorde...

Inam Ul Haq1*, Muhammad Ramzan1, Mansoor Khan Khattak1 and Ahmad Khan2

...m aestivum) cultivar (Pirsabak 2015) under deep tillage in silt clay loam (Pedocals) soil during 2019. The levels of planting technique factor were; 0 m high raised seed bed (P1), 10 cm high raised seed bed (P2) and 20 cm high raised seed bed (P3). Compost from domestic residues (C2), a combination of urea with compost (C3), and urea (C4) were compared with control, no N-fertilizer (C1) in integrated nutrient management factor. The result showed that the treat...

Nkana Kontchiachou J. Gwladys1, Kouamo Justin2, Vemo Bertin Narcisse3*, Mweugang Ngouopo Nathalie4, Wang-Baa Temoa Christophe1, Awantu Christian Funwi1, Semi Yam Alphonsius1, Kenne Noubissie Christèle5, Tendonkeng Fernand5

... in dry tubes, for the dosage of serum biochemical parameters. The main results revealed that the number of total white blood cells, lymphocytes, monocytes and granulocytes increased significantly (p<0.05) in guinea pigs supplemented at 0.5% green anise, compared to the control. The haemoglobin concentration, packed cell volume, mean corpuscular volume of haemoglobin, the number of red blood cells and the concentration of placitonin were comparable (p>0....
Bulkaini*, Syamsuhaidi, Yusuf Sutaryono, Djoko Kisworo, Sukirno, Sukarne, Tapaul Rozi
...ation of broiler chicken samples in each Regency was done by random sampling method. Research variables such as live weight, carcass weight and percentage, offal weight and percentage, edible offal weight and percentage, meat weight and percentage were analyzed using the Microsoft Excel program and regression correlation analysis. The results showed that carcasses with large size categories 77.67%, medium size 17.51% and sma...
Sara Mohamed Hemeda1, R.H. Sayed2, Hani Hassan1, Sheima A.E.2, Hassan Aboul-Ella3, R. Soliman3*
...ctam antibiotics in milk samples that exceed the permissible limit. In the newly developed kits, each involved β-lactam antibiotic conjugated with KLH was placed in the test line to compete for the gold chloride anti-β-lactams antibodies conjugate with the free beta-lactam antibiotics supposedly exceeding the permissible limit and existing in the tested samples or vice versa. The...

Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3

... resistance to 0.3% bile salt media, and antimicrobial activity against Escherichia coli: O157, Pseudomonas, Listeria inokua, and Klebsiella pneumonia; and (3) Molecular identification of LAB by analysis of the base sequence of the 16S RNA gene. The whey for this study was collected in Lasi Farm in Agam Regency, West Sumatra, Indonesia. The data was subsequently subJected to descriptive analysis. The bacteria obtained were rod-shaped that were catalase negativ...
Aiman Al Mufarji1, Abd El-Nasser Ahmed Mohammed1*, Rashid Al Zeidi2, Haitham Al Masruri3
...orded, as well as, blood samples were collected and analyzed for hematological parameters and plasma biochemistry parameters. The results indicated that heat stress negatively influenced the thermo-tolerance, hematological, and plasma biochemistry parameters. M. oleifera alleviated the negative effects of heat stress, as well as modulated thermo-tolerance responses, metabolites, and liver and kidney functions. M. oleifera supplementation with a rate of 50 and ...

Teguh Wahyono1,2*, Wahidin Teguh Sasongko3, Wijaya Murti Indriatama4, Setiawan Martono5, Slamet Widodo3, Widhi Kurniawan6, Muhamad Nasir Rofiq7

Oluwaseun Adetarami1*, Oluwatoyin Olagunju2, Babatunde Adebayo Oyebamiji1, Adegboyega Abel Odeyemi3 and Sina Basil Johnson4

...s of Nigeria. Multistage sampling procedure was used to select (160) respondents. The primary data were collected through structured questionnaire. For the data analysis, statistical tools such as budgeting technique and logitistic regression were used. Results of the budgeting technique showed that each farmer of the FBS approach had a mean profit of ₦ 81,134.65 with a cost benefit ratio of 1.09. The results of logistic regression revealed that the age of c...

Muhammad Luqman1*, Adeel Mustafa1, Sheer Abbas2, Muhammad Yaseen1, Muhammad Umer Mehomood1 and Raheel Saqib3

...om randomly selected 200 samples from two purposively selected districts Chakwal and Rawalpindi. Data were analyzed using Statistical Package for Social Science (SPSS). Data depicts that the maximum number of respondents were belonging to the age category of above 40 years of age and 70 respondents were recorded as illiterate. There were 75% of respondents (148) who claimed that there are multiple major income sources. The respondents who had a low level of po...

Syed Muhammad Amir1*, Shehzad Khan2, Asif Ali Abro3, Ehsan Inamullah4 and Tahir Mehmood5

...lternative activities in saved time, and enhances fuel energy consumption pattern. Biogas technology brings improvement in the economic condition in terms of reduced expenditure (on buying other fuel energy materials and on buying chemical fertilizers), and in terms of higher earnings from selling trees. In light of the findings from this research, biogas technology has been proved to have great potential to improve the quality of life of farming households wh...

Saima Mehar1 and Salma Javed2*

...omponents employing bioassay-directed fractionation of both extracts. Concentration of the extract was directly proportional to the mortality of second stage juveniles, the resulted, six withanolides comprising coagulin E, G and J, withaperuvin C and triterpenoid, betulinic acid and the pure isolated bio active constituents also showed the 100% significance mortality with the constant concentration of 1 and 0.5 mg/lagainst the M. incognita. The present study r...

Amal Ramadan Fawy1, Hussein Yousef Ahmed2, El-Sayed S.E. Shabana3, Mohamed Abdelfattah Maky4*

...lity. Twenty beef burger samples were collected from Qena mar­kets, Egypt. They were divided into four groups based on their price; A, B, C, and D, with group A being the cheapest while the group D being the most costly. Proximate and quality analysis were conducted. Group D of Egyptian beef burger had a greater protein than other groups as well as being the best in terms of quality. Furthermore, six beef burger formulations with different amounts of beef ...
Widiastuti Ardiansyah1,3*, Osfar Sjofjan2, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2
...ction performance of Mojosari ducks. The turmeric consisted bioactive substances, while Lactobacillus sp. produced lactic acid bacteria. The 280 laying ducks used were Mojosari ducks aged 20–28 weeks reared for eight weeks on the experimental diet. The Curcuma longa flour used was produced by Balai Materia Medika, Batu District and the liquid probiotics used contained Lactobacillus sp. bacteria in concentration of 1.4 ...
Amreen Zahra1*, Mushtaq A. Saleem2, Hasnain Javed3, Muhammad Azmat Ullah Khan4 and Abdul Rauf Shakoori4
... Kot Imrana Sargodha, Faisalabad and Gujrat. Drug resistance mutations such as T12A, I13A, K14R, I15V, K20I, T31S, E35D, M36I, N37D, R41K, K43X, R57K, Q61H, H69K, K70R and L89M were observed across several sites across the whole length of PR region among the isolates. A significant accessory resistance mutation V32I conferring a low level of resistance against non-nucleoside reverse transcriptase inhibitors was observed among two isolates. This kind of genotyp...
Dalia M. Aboelhassan1*, Inas S. Ghaly1, Noha E. Ibrahim2, Nermeen M. Shaffie3, Mariam G. Eshak1, Aboelfetoh M. Abdallah4, Ibrahim M. Farag1
...the genetic study, the assaying of expressions of IkBα and COX-1 genes in liver tissues, as well as the expressions of mdr1a and COX-1 genes in kidney tissues are evaluated. In addition, the histological architectures of these organs are examined. Bisphenol A (BPA) treatment causes over-expressions of the above mentioned genes, and induces massive damage to the histological architectures of liver and kidney tissues. In contrast, FCE treatment with differ...

Alsi Dara Paryuni1, Soedarmanto Indarjulianto2, Tri Untari3 and Sitarina Widyarini4*

...n of fungi. Skin scrapes samples were cultured onto Sabouraud’s dextrose agar medium. Hair and skin scraping were conducted at the day-1 (pre-treated with ketoconazole). Subsequently, infected cats were treated with topical application of 2% ketoconazole cream, twice a day for 21 days. Results showed that all of cats positive infected with Microsporum canis. Feature of the skin of cats infected by Microsporum canis demonstrates erythema, scale, crust, an...
Aiman Al-Mufarji1, Abd El-Nasser Ahmed Mohammed1*, Haitham Al-Masruri2, Rashid Al-Zeidi3
...ude “proteins, polysaccharides, lipids, PUFAs, vitamins, pigments and other bioactive compounds”. The microalgae composition varies depending on species and nutrient availability for production. Genetic engineering might be used for production invaluable microalgae compounds. Microalgae consider a promising source of omega-3 fatty acids (n-3 FAs), which have beneficial effects for humans and animals. Knowledge for microalgae effects on productive, ...
Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1
...attle. Serum and ovarian samples were collected from cattle during the year of 2019. RT-PCR, viral isolation, sequencing, and phylogeny revealed that the primary causative agent was HoBi-like Pestivirus. Our HoBi-like Pestivirus strain, Egypt-020-1ncp, BVDV-Egypt-020-3ncp and BVDV-Egypt-020-2ncp, shared 99.6% homology with BVD3- B1-AU, 99.2% with BVD3- B5-3-MX and BVD3-G2-BR, 98% with BVD3- Hobi-like virus

Amany R. Sultan1, 2, Gamal A. Morsi2, Hoda El-Fayoumi1, Abdel-Azeem S. Abdel-Baki1*

... June and 10th July. The samples of T. absoluta collected in the present study were parasitized by Bracon sp., Pteromalus sp. and Eulophus sp. with maximum rate of parasitism reached to 51% with 5 peaks similar to these of T. absoluta. Therefore, we suggest that these parasitic species could be used in biological control of T. absoluta.
 
Keywords | Solanum lycopersicum, Biological control, Parasite, Prevalence
...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...irus-5 (BoHV-5) have the same antigenic and genetic relatedness. BoHV-1 is one of the viruses that incriminated in respiratory disease; affects the genital tract and causes significant economic losses to domestic cattle in Egypt. Although BoHV-5 mainly causes encephalitis in calves, the virus is excreted in nasal discharge under stress factors, so herpesvirus latency reactivation cycle is the hallmark of the disease. In the ...
Genevieve R. Tabon1,2, Noraine P. Medina2, Mary Rose D. Uy-De Guia3, Claro N. Mingala2,3*
...s showed that all of the samples were found to be of AA genotype which is not the favorable genotype according to previous studies. The litter sizes of the PNP were also determined. Results showed that the genotype of ESR1 gene present in PNP is homozygote AA. The average litter size of the 30 PNP is 7.07 which is relatively higher than the average litter sizes of Nepal native pigs which ranges from 2 to 6. Moreover, the average litter size of the Philippine b...

Sajid Ali1*, Shahen Shah2, Muhammad Amin2, Asad Ali Khan2, Dawood Ahmad4, Ikram Ullah5, Faiq Ahmad2, Sajjad6 and Sikandar Azam1

...s Section Harichand- Charsadda, Pakistan. The field experiment was conducted on 7 July 2021. Two levels of P (90 and 120 kg ha-1) and three levels of Zn (0 i.e., water spray only, 2.5 and 5 kg ha-1) were applied at three growth stages, full at vegetative stage (V6), full at reproductive stage (R2) and ½ at V6 and ½ at R2 stage along-with an overall control were used in the experiment. Randomized complete block design was used with three replicati...

Imtiaz Ahmed*, Imran Khan and Zia Ud Din

...and composite test bread samples were performed using standard methods. Folin-Ciocalteu, Aluminium chloride, and Colorimetric assays (ABTS and DPPH) were followed to determine total phenolic content, flavonoid contents and antioxidant status respectively along with“in-vitro starch digestibility”. The ash, fiber and protein content increased significantly while the carbohydrate content had significantly lower valu...

A. El-Shemy1*, Hoda M. Mekky2, M. A. Bosila2, A. M. Allam1, Kh. M. Elbayoumi2, M. M. Amer3 

...sses. We collected liver samples from three duck farms representing Muscovy and Pekin breeds at ages ranging from 2 to 4 weeks and located in two Egyptian governorates between August and October 2018. The affected ducks were suffering from a short course of nervous signs, and mortality was high, reaching 75% within 3–4 days. Implementation of one-step reverse transcription polymerase chain reaction (RT-PCR) for processed livers using specific primers aim...

Ngoc Tan Nguyen1*, Minh Thanh Tram1, Thi Thu Pham1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Nguyen Khang Duong4 

...n on their origin. Blood samples were taken from two local goat breeds in Ninh Thuan (De Co = CO and Bach Thao = BT, 10 samples each) and three other exotic goats including Saanen (SA; 10 samples), Red Boer (RB; 10 samples) and White Boer (WB; 9 sa...

Khuzeema Tanveer1, Muhammad Shahid Mahmood1,*, Muhammad Ashraf1 and Ahrar Khan2

...e products a hemolytic assay was performed which showed a variable decrease or increase in red blood cells cytotoxicity caused by bacterial treated or untreated dye solutions. The current study suggests that some of the bacteria have the potential to degrade a number of textile industry dyes and could be exploited for xenobiotic removal.

...

Ziaul Islam1*, Asad Sultan1, Sar Zamin Khan1 and Sher Bahader Khan2

... dietary treatments. A basal diet formulated with corn-soybean meal (CON; without any additives) served as control diet and fed to birds in control group. Birds in other four groups were given same basal diet mixed either with zinc bacitracin (150 mg/kg diet; ZB-150); organic acids (200 mg/kg feed; OA-200); essential oil (150 mg/kg feed; EO-150) and or a combination of organic acid (OA) an...
Baila Ahmad1, Muhammad Ammar Khan1*, Zulfiqar Ahmad1, Rana Muhammad Bilal2 and Asghar Ali Kamboh3
...se concerns. Breast meat samples were obtained from the broilers given zinc (Zn from ZnSO4) supplements (T0, T1, T2, and T3 @ 0, 10, 20 and 40 ppm, respectively of finisher diet from 21 to 42 days). Initially, the pH, expressible moisture (EM), water-holding capacity (WHC), instrumental color, drip loss (DL) and cook loss (CL) were measured. Afterward, the effect of processing (25°C, thermal treatment for 15 min, refrigeration for 3 d, and pressure treatme...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...g kits of progesterone assay, and to confirm the diagnosis of COD, mostly trans rectal palpation and trans rectal ultrasonography methods have been used. COD should be treated by using different treatment protocols such as hormonal, medicinal and homeopathic medicines.

...

Xiaowei Huang1, Jinji Wang1, Zhun Yu2, Minghua Duan1* and Zhe Lin1*

...;">Angelica sinensis polysaccharide (ASP) is a biomacromolecule that isolated from the roots of Angelica sinensis. This study aims to investigate its protective effect on kidney injury and its influence on BMP-7/Smads/TGF-β1 signal pathway in irradiated rats. Total 60 Sprague Dawley (SD) rats were randomly divided into 5 groups: the normal (normal saline), model (normal saline), and l...

Xinjun Zhang1,2, Pengyong Wang1,2, Huimin Ju1,2, Yanhong Wang1,2, Yang Yang1,2* and Guoqiang Zhu1,2*

...ulence-associated genes (sat, cdtI, cnf1, hlyD), iron uptake system encoding genes (iroN, iut, ireA) were investigated, and the ability of biofilm formation was examined. In addition, UPEC in invading T24 cells, cytotoxicity, immune response and synthesis of proinflammatory cytokines were discussed. The results showed that fimH was 100% detected, followed by iut, iroN, and focA, with the detection rates of 71.4, 57.1 and 57.1% respectively; while papG allele I...
Tariq Mehmood1, Muhammad Arif Khan1, Ayesha Safder Chaudhry1, Kamran Ashraf2, Muhammad Mohsin Ali1,3, Muhammad Faisal Ayoob3*, Mudassir Hussain4, Farrukh Saleem3 and Muhammad Fayaz3
Muhammad Zaeem Abbas1, Khurram Ashfaq1, Amjad Islam Aqib2, Mughees Aizaz Alvi1*, Muhammad Haleem Tayyab1, Muzafar Ghafoor1, Muhammad Usman Naseer3, Ali Abbas4 and Ali Hassan1
...s regard, milk and urine samples were individually collected from 96 buffaloes and analysed by Ross Modification of Rothera’s test and Abbott Precision Xtra™ meter to assess the ketone bodies levels. We observed a total of eight out of 96 (8.33%) buffaloes positive for ketosis. The peak prevalence (13.3%) of ketosis was detected during the third week of lactation. Animals with body condition score >4 (15%), buffaloes yielding 18 liter milk in a ...

Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider

... first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS, 0.5mM + 10mM CYS, 1mM GLU + 5mM CYS, 1mM GLU + 10mM CYS and Control). At pos...

Abdul Hafeez Mastoi1, Muhammad Rashid Nizamani2, Shafique Ahmed Memon3, Jun Jiang1, Xiang-Shun Hu1* and Tong-Xian Liu1*

... cultivars of pea, Pisum sativum L., was determined in laboratory testing. Of the 5 pea cultivars and 5 temperature levels included in our study, the highest values of the population parameters of net reproductive rate, intrinsic rate increase, finite rate increase, generation time, and fecundity of A. pisum was observed at 27°C, regardless of pea cultivar. In decreased as temperature increased at and above 30°C for each cultivar. Furthermore, differen...

Jawaid A. Khokhar*, T. J. Ursani, Fareeda Memon, Samina Malik, Asif Soomro and Fareeda Shah

...ntomology. The collected samples were kept at an Average temperature and humidity between 28.2 ± 0.47 to 38.78 ± 0.47 0c, 57.6 ± 0.55 to 72.6%, respectively.

...

Khalid Javed Iqbal1, Irfan Baboo2*, Arshad Javid3, Noor Khan4, Hamid Majeed5, Azra Anwer1, Zahid Farooq2, Muhammad Altaf6 and Moazam Jalees7

...an be beneficial for the safety of aquatic environment and management of aquatic toxicity.

...

Veronica Ngozi Emenuga1, Clara Idara Eleazar2*, Seto Tunrayo Aladenika1  

... examined alongside with samples of milk and sawdust beddings. Culture, identification and characterization of isolates were carried out using standard methods. Antifungal biogram was performed. Result showed that all the cattle and the bedding tested positive for fungi, while 41(82%) of the milk samples had fungal contamination. Rate of isolation from cattle, bedding and the milk was no...

Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi 

... Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% and 52.76± 8.88%, and 53.00&pl...

Tooba Ather1, Muhammad Rafiq Shahid2*, Muhammad Ahsin Ayub3, Javed Iqbal2, Muhammad Asim Bhutta2, Muhammad Akram2, Naveeda Anjum4, Muhammad Rizwan6, Hafeezur Rehman5 and Umar Farooq5

...gulator (IGR) were bio-assayed against 3rd instar of CMBat Integrated Pest Management Laboratory, Department of Entomology, University of Agriculture, Faisalabad, during 2016. The results revealed that mortality of 3rd instar of P. solenopsis increased with increase in concentration of Pyriproxyfen and Buprofezin at three days of post application interval (PAI). Pyriproxyfen 0.5% and Buprofezin at 2% concentration induced mo...

Khalid Khan1*, Ahmed Farhan Saeed2, Sanam Wagma Khattak3 and Saima Liaqat4

...tan. A multistage random sampling method was adopted to select the samples of 75 farmers from five tehsils of Lasbela namely Hub, Gadani, Uthal, Dureji, and Bela. Around 15 farmers were randomly selected and questioned in each of the tehsil. To achieve the objectives of study nonparametric correlation coefficient test and regression analysis are carried out. The study accentuated various factors which are responsible for the...

Waleed Asghar1,2*, Ahmad Mahmood1, Farhan Iftikhar2,3, Bushra Ahmad4, Rehmat Ullah5, Muhammad Bilal5, Abdul Latif6*, Muhammad Arsalan6, Madeeha Khan6, Rizwan Latif7 Muhammad Ehsan7

...onal fertilization could save chemical fertilizer’s contribution and minimize soil deterioration by overuse of chemical fertilizers.

...

Masudul Haq Wani and Arshad Bhat*

...u and Kashmir during the same period it has reduced from 1.06 to 0.58. The study shows that city populace within the area has accelerated from 7.42 to 33.00 percentage as towards 10.84 to 38.70 at national level. During last two decades, developed districts based on per capita income such as Srinagar and Jammu have shown low growth in urban population. The study make a sound point that in the UT the up rise in urban population was not observed in old cities li...

Dede Kardaya*, Dewi Wahyuni, Elis Dihansih 

...ation is considered necessary. Leaves of Asam gelugur (Garcinia atroviridis) contain hydroxy citric acid and other active substances that function as antioxidants, so the use of them in ration is expected to improve the meat quality of spent layer ducks. Sixty spent layer ducks were fed four rations including commercial rations (R1), nonconventional rations + 2% Asam gelugur ...

Mostafa El-Sebelgy1*, Hanafy Madbouly2, Sabry Tamam2, Nagwa Ata1, Kawther Zaher1

 

...were detected and at the same time, integration events were examined in this study. This research work entails unpredicted integration of foreign non-BTV viral proteins (NS1, NS3 and VP3 of AHSV and NS3 for EEV). The multiple sequence alignment models elucidated the extent of the integration into the BTV genomic backbone with a resultant insight into PCR negativity. The result confirms the unexpected integration of some segments from equine viruses AHSV and EE...

Muhammad Ajmal Khan1, Muhammad Yahya2*, Ali Hazrat2, Javed Khan3, Saeed Jan4, Tabinda Nowsheen2 and Inam Ullah5

...gh agar well diffusion assay (small wells were made with cork borer and 6μl of plant extract was added to the wells). The antioxidant activity of methanolic leaves extract was evaluated through a well established protocol 2,2-diphenyl-picrylhydrazyl hydrate (DPPH) at various concentrations (50, 100, 150, 200, and 250 µL). The phytochemical analysis of methanolic leaves extract of T. camphoratum showed 2.80% alkaloids, 4.90% flavonoids and 1.80% terpen...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2 

Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg  

... wet seasons. Wastewater samples from five slaughterhouses and water samples from water sources next to the abattoirs were examined using standard procedures. The bacteria isolated were E. coli, P. aeruginosa, K. pneumoniae, E. faecalis, and S. dysenteriae. Bacterial concentrations cfu/100ml of abattoir effluents ranged from 3.17 × 106 of TC. 3.94x104 of FC, 2.84x104 of E. faecalis, ...
Ramadan Sary, Asmaa M. Ibrahium*
...ones of face formed of nasal, quadrate bone, zygomatic, palatine, vomer, premaxillary, maxillary and ethmoid. The cranium and viscerocranium demarcated from each other by orbital cavities. The mandible composed of five ill distinguished bones; dental, splenial, angular, supra angular, and the articular.
 
Keywords | Egyptian owl, Skull, Morphology, Radiology, Computerized tomography
...

Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6 

 

...mL of phosphate-buffered saline (PBS) via the intramuscular route. At week 2, both non-vaccinated and the vaccinated groups were challenged intranasally with 2 mL of bacterial cell suspension containing 105 colony-forming unit (CFU) of M. haemolytica serotype A2. Blood samples were collected on weekly basis. The results revealed that the plasma progesterone concentration increased signific...
Gamilat A. Elsaid1, Weam Mohamed Baher1*, Eman Shukry1, Abeer E. Abd El. Ghafar2, Marwa Shalaby1
...nd 3.60 ± 0.25 in samples collected from street vendors. The prevalence rates of E. coli were 20%, and 60% in kareish cheese samples collected from grocery stores and street vendors, respectively. The identified E. coli serotypes were E. coli O2:H6, E. coli O26:H11, E. coli O55:H7, E. coli O78:H-, E. coli O111:H4, and E. coli O119: H. All isolated harbored 16S rRNA specific gene for E. coli. The expression of shiga to...
Eman M. Aboelela1, Mohamed A. Sobieh2, Eman M. Abouelhassan3, Doaa S. Farid4, Essam S. Soliman2*
...identified Rhipicephalus sanguineus ticks, Ctenocephalides canis flea, Heterodoxus spiniger lice, and Sarcoptic scabiei mites in dogs, Otodectes cynotis ear mites, and Ctenocephalides felis flea in cats. Prevalence of parasitic infestations in dogs and cats showed strong associations with the breed, sex, age, coat color, housing system and pattern, type of food, and type of infesting external parasites concerning seasonal variations.
 
...

Peyman Mohammadzadeh1*, Aida Rasuli2, Bahman Noroozi Gorgani2, Sajjad Mohammadi3

...scale less than 8, and basal nucleus ganglia dysfunction. A 40-ml intracerebral hemorrhage occurred followed by severe blood aspiration and asphyxia.
 
Keywords | Choroid blindness, Neuroinflammation, Neuro-ocular toxoplasmosis, Red Kangaroo, Traumatic Brain Injury, Necropsy, Histopathology
...

Ayesha Khan*, Mohammad Tariq Aziz, Urooba Pervaiz and Muhammad Zafarullah Khan

...ancement in district Charsadda, the universe of the study. For selection of sample multi stage sampling method was used and respondents were selected from three villages namely Hajizai, Katozai and Batagram of tehsil Shabqadar. A sample of 112 respondents was drawn through proportional allocation technique based on 10% of total 1120 registered farmers. A...

Zahin Anjum1*, Farhat Shehzad1, Shaista Ali1, Sumbla Yousaf2, Amina Rahat1 and Hamid Ullah Shah3

...arbecued mutton and beef samples available in district Peshawar, Pakistan. In addition, the effect of various grilling methods on PAH levels was evaluated. Mutton grilled with three grilling procedures, were analyzed for its PAHs content. A total of 120 samples were collected from local food vendor spots or general restaurants, and control samples of gas and electric grilled meat

Amina Batool1*, Saba Aleem1, Ali Nawaz1, Muhammad Imran Khan1, Waheed Arshad1, Muhammad Aslam2, Shiraz Ali3 and Muhammad Zeeshan3

...er count (405.67 m-2) at same level of seeding density. In contrast, 16FJ17 stood second in all parameters except root length and number of tillers m-2. The value for grain per spike (40.0) and shoot length (10.55 cm) is at par with Fatehjang-2016 and Dharabi-11, respectively at 120 kg ha-1 of seeding level. It is evident from the results that wheat variety Fatehjang-2016 can effectively be planted at an optimum seed rate of 120 kg ha-1 for general cultivation...

Zohair S. Mulla1*, Mostafa M. Abdelhafeez2 

...f, beef burger, and beef sausage retailed in Saudi Arabia. Bacteriological examinations involved the estimation of total bacterial count (TBC), total psychrophilic count (TPsC), most probable number (MPN) of coliforms, and MPN of E. coli. In addition, isolation, and identification of shiga toxin producing E. coli were further conducted. An experimental trial was done for improvement of the bacteriological status of the groun...

Iqra Ejaz1, Saima Chaudhry2 and Sarah Ghafoor1*

...(1mg/mL) into buccal mucosa of animals daily for 8 weeks. The 24 male Wistar rats were equally divided into two groups; six rats (group 1A) were injected with normal saline (control group) while 18 rats (groups 1B, 2A and 2B, experimental groups) were injected with bleomycin. The six rats of control group (1A) and the six rats of experimental group (1B) were sacrificed after week 4 and wee...

Lin Li1,2, Pingsheng Ye1, Xi Chen1, Shuping Yan1 and Yuanshu Zhang1*

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

...e-linked immunosorbent assay (ELISA) method based on the SAG2 gene of Toxoplasma gondii (T. gondii) to improve the detection of toxoplasmosis in pet cats. Escherichia coli BL21 (DE3) was transformed with a prokaryotic expression vector, pET-21a-SAG...

Muhammad Furqan and Zulfiqar Ali

...ext-align: justify;">Pheasants are bioindicators of our ecosystem and their population is declining. We investigated diet composition and threats to kalij pheasant Lophura leucomelanos to prepare conservation and management strategies in Azad Jammu and Kashmir, Pakistan. The diet analysis was done through crop contents of kalij pheasants, threats were assessed through field surveys, commun...

Shambel Boki Dabi

...and rainy seasons, water samples were collected at a depth of 10 to 15 cm in plastic bottles from each site of three streams for laboratory study. Water quality parameters such as CO2, Chloride, and alkalinity were instantly assessed using the titration method, while water turbidity, temperature, water pH, and dissolved oxygen were measured directly on site. A simple test kit was used to evaluate nitrite and ammonia levels. The result revealed a high level of ...

Mudssar Ali1*, Muhammad Awais Ahmad1, Asif Sajjad2 and Shafqat Saeed1

..."text-align: justify;">Sesame is ranked among the top ten traditional oil seed crops of Pakistan. The present study is aimed to compare the effectiveness of native honey bees and solitary bees for better seed production of sesame and future conservation of effective insect pollinators. The sesame crop was grown during two years (2019 & 2020) at the research farm of MNS- University of A...

Asma Fatima*, Ghulam Abbas and Shahnaz Rashid

..., Sindh, Pakistan. Water samples were collected monthly (Jan-Dec, 2019) from different sites of the ponds and studied the total abundance of plankton in each month. During the study, physicochemical parameters of the pond water like temperature, pH, dissolved oxygen, salinity, transparency, ammonia, phosphates, nitrates, potassium, calcium, total alkalinity and total hardness were determined. Overall, 61 species of plankton ...

Qian Chong1, Muhammad Mohsin2*, Zhu Ting1, Chen Qiqi1 and Ana Mehak3

...antity of T. lepturus to safeguard this fishery resource for the future. Besides, further analogous research is strongly advised prior to making or executing any administration strategy.

...
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
...ions in proximal tibiotarsal bone. The aim of present study was to investigate the antioxidant capacity of FK228 and role of vascular endothelial growth factor (VEGF) and Flk-1 genes in thiram induced TD. One hundred and fifty broiler chicks were equally divided into 3 groups: control; thiram fed; and FK228 treatment group. Expressions of VEGF and Flk-1 genes were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) on day 10 and 14 post-hatc...
Rida Haroon Durrani1, Ali Ahmad Sheikh1*, Masood Rabbani1, Muti-ur-Rehman Khan2, Aneela Zameer Durrani3, Salman Ashraf1, Muhammad Anas Naeem1, Muhammad Usman3 and Tauqeer Mahmood4
...>Despite advances, avian salmonellosis pose a major challenge to the poultry health, associated with high production losses. The study aimed to investigate the effect of oro-nasal phage delivery on bacterial population. As prophylaxis, oral phage delivery via drinking water and feed resulted in a significant (P ≤ 0.05) reduction in bacterial numbers with a mean log101.43, log101.17, log101.92 and log101.625 on 3rd and 6th...

Mohammed A. Almujtaba1, Turky Omar Asar2, Salma Naqvi3, Vikas Kumar4, Fahad A. Al-Abbasi1, Abdulbasit I. Al-Sieni1 and Firoz Anwar1*

...otocol, the animals were sacrificed, heart preserved, and blood collected for biochemical estimations. Serum levels of cardiac biomarkers, CRP, Fibrinogen, BUN and creatinine increased, while zinc, IL-10 and angiotensin levels decreased significantly in DC as compared to NC. Interestingly, renin level also decreased in DC group. Heart tissues from each group were examined for histological changes. Altered serum levels of the biomarkers were restored on treatme...

Abid Ali1*, Safia Naureen Malik3, Muhammad Akmal2, Hafeez Ullah Rafa2 and Abid Subhani3

...total of 565 groundwater samples were collected from farmers’ tube wells during random survey of four tehsils of Gujranwala district, i.e., Gujranwala, Kamoke, Wazirabad and Noshera Virkan. The water samples were analyzed in Soil and Water Testing Laboratory Gujranwala for electrical conductivity (EC), sodium adsorption ratio (SAR), residual sodium...

Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3

...e Linked Immunosorbent Assay (ELISA). For purification of fraction, bacterial fresh cultures were centrifuged at 3000 rpm / 20 min and were homogenate in phosphate buffer saline and centrifuged at 15000 rpm / 20 min that known as the crude antigen. It entered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Che...

Tasya Nur Karina1, Osfar Sjofjan2, Tri Eko Susilorini2, Muhammad Halim Natsir2*

...evels as follows, T0 = basal diet + 0% control, T1 = basal diet + 2.5% date palm flour, T2 = basal diet + 5% date palm flour, T3 = basal diet + 7.5% date palm flour, T4 = basal feed + 10% date palm flour. The variables observed were production performance and egg physical quality. This research used 125 laying hens age...

Murat Durmuş* and Nazan Koluman

... lambs of the Cukurova Assaf type (75% Ost-Friz + 25% Awassi) and 10 head kids of Improved Cukurova Boer type (10% Native Hair Goat + 90% Boer) were used as animal material in the experiment. Kids and lambs were housed in two different paddocks during the eight-week trial period and performance data such as feed consumption and live weight gain were recorded during the trial. Four head animals, of which closest to the average live weight of each group in the f...

Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5

... g, respectively), or bursal (1.55+0.79 g and 1.63+0.21 g, respectively) body weight ratios. However, lincomycin did adversely affect bursal (0.91+0.12 g), but not splenic (1.21+0.23 g), thymic (3.52+0.36 g), or hepatic (23.72+1.78 g) body weight ratios. The total viable bacterial counts per gram of feces before and 120 h after medication were significantly different (p<0.05). Interestingly, the feed efficiency of non-med...

Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6

Idrissa Sacko1, Madou Dao1, Souleymane Sanogo1,2*, B. Michel Orounladji2, Kaly Diakité1, Diakaridia Traore3, Mamadou D. Coulibaly1, Amadou B. Cisse1

Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2

...e of 16 liver and spleen samples out of 20 (80%) from aborted fetuses were positive for EHV-4, but all were negative for EHV-1. Virus isolation trial for EHV-4 were done, eleven samples out of 20 (55%) on the CAM of ECE were positive. The virus glycoprotein B (gB) fragment (580pb) was amplified in selected isolates using the nested PCR (n-PCR), sanger sequencing of gB from three isolates w...

Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1 

...prominent making foods unsafe and insecure. Food and feed contamination by mycotoxins are among key factors creating food insecurity. There are various forms of aflatoxin (AF) occurring in foods of plant origin (B1, B2, G1, and G2) and animal origin (M1 and M2). Aflatoxin M1 (AFM1) is a geno-toxic carcinogen and is less lethal but it is still cytotoxic. The present study was conducted on prevalence and quantification of AFM1 in raw buffalo milk in Sindh provin...

Xinran Wang1,2 and Lizhi Zhou1,2*

...arge flock could provide safety by the vigilance partners through coordinated and synchronous vigilance modes. In this paper, we aimed to shed light on behavioral strategies adaptive to anthropogenic disturbances. Vigilance behavior of wintering Grus monacha was observed to assess the effect of traffic intensity on their vigilance mode at Shengjin Lake, a Ramsar Site in China. Disturbance intensity was divided into three lev...

Agus Mulyono

...ppearance that looks the same, especially the color of their fur. Limited information regarding the characteristics and behavior of male and female Java sparrows can cause problems regarding sex determination, causing delays in normal mating. Further impact on the crossbreeding efforts of Java sparrow. This study aims to determine the sex detection process of Java sparrow through color and texture analysis of Java sparrow beak image with classification method ...
Samreen Fatima* and Muddasir-Uddin
...esults show that both in-sample and out-sample RMSE, MAE and RMAE are minimum in GARCH (1, 1)-M-ANNs models. Furthermore, empirical analysis reveals that the forecast result of GARCH (1, 1)-M-ANNs models of all three forms give a similar result. The results obtained here are useful for the market practitioners, policymakers and investors. 
...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
... by various cell-based assays such as neutral red assay and BrdU cell proliferation assay showed that higher concentrations were lethal for the cancerous cells. IC50 was found to be 12.6 µM. Further contextualization of the therapeutic potential of G9a inhibition was provided by expression analysis of the G9a gene and genes associated with cell proliferation, tumor suppression, and a...

Zannatul Ferdous1, Abdul Goffar Sarder2, Mustasim Famous3*, Mahfuza Ferdous4, Shahabuddin Ahmed5 and Md. Amir Hossain6

...tal groups was about the same. Weekly observations were recorded for live body weight up to 35 days and blood parameters of birds at day 14th, 21th and 35th days. The result showed comparatively better live weight in T4 which is treated with Kalojeera and Ginger having a satisfactory feed conversion ratio (FCR) of 1.68. The better feed conversion ratio (FCR) value was recorded by chicks fed diet supplemented with antibiotics...

Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1 

...follicular fluid, granulosa cells, ovarian stroma and blood vessels), the fallopian tube (covering epithelium, sub mucosa and musculosa) and at the uterus (epithelium, endometrial glands, blood vessels and endometrial stroma). While in the acute cases, the positive BVDV antigen could not be observed in the primordial follicles, blood vessels or endometrial glands.

Keywords | Bovine...

Gawhara Ahmed-Abdelmonem1*, Zeinab Aboezz2, Ahmed Habashi1, Saad Sharawi2 

...aks in Egypt. Thirty-six samples (26 blood and 10 oral epithelial tissue samples) were obtained from suspected cattle in three Egyptian governorates during 2019-2020. The samples were screened for FMDV by means of real‑time RT‑PCR that showed nearly 86% (n=31) of the examined samples to be FMDV positive. Virus isolation was carried out on ten of the ...

Aysam Fayed1, Mohga A Ibrahem2, Soha A. Hassan3, Mohammed Abdalla Hussein4*, Tamer Roshdy1  

...s determined using blood samples, and liver tissue samples were used to determine glucose, insulin, leptin, TC, TG, HDL-C, ALT, AST, MDA, TNF-α, PCO. The HFD administrated rats resulted in increased total body weight, glucose, insulin, leptin, cholesterol, triglycerides, MDA, ALT, AST, TNF- α and PCO levels, as well as SREBP1 gene expression, according to the findings. However, our findings revealed a significant...
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
...ease virus (NDV), the causative agent of Newcastle disease (ND) in chickens. Owing to its highly conserved nature among NDV strains and also due to its pivotal role in the viral life cycle, the M protein can be employed as a promising diagnostic antigen for reliable detection of NDV infection in chickens. In the present study, we have devised a strategy for extraction and solubilization of the NDV M protein from Escherichia coli in a single step using a non-io...

Umar Farooq Gohar, Attia Majeed, Bushra Muneer* and Hamid Mukhtar

... (Podophyllum hexandrum) samples were collected from different localities of Pakistan. About 261 fungal strains isolated, among them 22 strains had the ability to produce podophyllotoxin. Maximum podophyllotoxin production (88.14µg/ml) was shown by fungal strain P-18, which was later identified as Penicillium sp. through 18S rRNA gene sequencing. Optimum growth conditions and parameters such as fermentation medium, pH, temperature, inoculum size, carbon ...

Muhammad Hassan Naseem1, Muhammad Mansha1*, Muhammad Triq1 and Peter C. Thomson2

...onsent. Peripheral blood samples of case and control subjects were analysed to evaluate the effect of shisha smoking on blood pressure, BMI (body mass index) and blood parameters, namely HB (hemoglobin), RBCs (red blood cells), WBCs (White blood cells), PLTs (platelets), MCV (mean corpuscular volume), MCH (mean corpuscular hemoglobin), MCHC (mean corpuscular hemoglobin), MPV (mean platelets volume) RDWc (red cell distribution width), PDWc (platelets distributi...

Zaidi Zona, Zulfiqar Ali*, Rida Ahmad and Irfan Zainab

... concentration, and high salinity manifested to be the best habitat conditions downstream of Thakot area. To conserve the richness and integrity of fish communities, it is recommended that instream flow evaluations to include target species that occupy flow-sensitive habitat categories.

...

Xiaoli Yao1, Xiaofang Yue2, Yue Yang3 and Guobin Zhang3,*

...ined with argatroban are safe and effective in the treatment of acute cerebral infarction.

...

Xiaoli Yao1, Xiaofang Yue2, Yue Yang3 and Guobin Zhang3,*

...ined with argatroban are safe and effective in the treatment of acute cerebral infarction.

...

Maria Endo Mahata1*, Hamdan Sukri Lubis1, Takayuki Ohnuma2, Yose Rizal1 

...ral pellet binders and dosage in producing pellet made of Miana plant as poultry feedstuffs. This experiment was administered in a completely randomized design with two factors. The first factor was natural pellet binders composed of brown seaweed Sargassum binderi, taro tubers (Colocasia esculenta (L.) Schott), and tapioca flour (Manihot utilissima), and the second factor was dosage (1.5; 3; and 4.5%) of natural pellet...

Nguyen Hai Quan, Nguyen Huu Van*, Nguyen Thanh Thuy, Vo Thi Minh Tam, Le Duc Thao, Le Duc Ngoan 

... added to CTL (SCTL); Cassava byproduct added to CTL at 5, 10 and 15% (CB5, CB10 and CB15, respectively); Molasses added to CTL at 2.5, 5.0 and 7.5% (M2.5, M5.0 and M7.5, respectively). Samples were collected at day 0, 7, 14 and 21 for pH and ammonia, and day 0 and 21 for chemical analysis. Results indicated that after 21 days of ensiling, the pH value of silage in 8 treatments were lower than 4.5. There was no effect of additives and their level inclusions on...

Hanan Saad El-Samahy, Amani Abd El-Naby Hafez, Mohamed Talat Ragab, Disouky Mohamed Mourad*  

...ifficult isolation of causative bacterial agents, weight loss, reduced fertility, and hatchability, so this study aimed to determine the bacterial causative agents using multiplex PCR as an accurate rapid diagnostic technique. Exudates of swollen infra-orbital sinus were aspirated and aseptically collected from affected turkeys of seventeen flocks and examined by multiplex PCR against Escherichia coli (E. coli), Mycoplasma g...

Mutassim Abdelrahman1, Ibrahim Alhidary1, Majdi Bahaddi1,2, Mohsen Alobre1,3, Riyadh Aljumaah1 and Rifat Ullah Khan4, *

...pectively). Although the same trend was reported for Mn in the liver, no difference between T1 and T2 groups was observed. Moreover, numerical gradual increase coordinated with increased zeolite level (T2) was detected in concentration of Potassium in the liver, kidney, and rumen fluid, but not in meat. Significant differences (P < 0.05) were reported for Mn level between treatments, in which higher values were found for lambs from T1 and T2. Introduction o...

Hazem E. M. Hassanien1*, Awad M. M. Mahmoud2, Elsayed M. Abdel-Raouf1, Nabil M. Eweedah1 and Midhat. N. Nassif3

...(DCAD) motivates a compensated metabolic acidosis, stimulating calcium (Ca) absorption and mobilization before calving and decreasing clinical and subclinical hypocalcemia postpartum. The objective of this trial was to determine the effects of varying pre- and postpartum DCAD, mEq [(Na + K - Cl + S)]/ kg of dry matter on blood total Ca, ionized iCa, blood, and rumen metabolites, and endocrine status in prepartum and postpartum phases. Forty-eight multiparous H...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...lobacter species. The causative agent is Campylobacter that asymptomatically colonizes broilers during development and contaminates it during slaughter. Outbreaks mostly start from the ingestion of contaminated poultry products or infected water. Reducing colonization of Campylobacter jejuni in the gut can be useful in decreasing the contamination of the poultry. Different organic acids display potential as a substitute of antibiotics. These not only improve p...

Malik Fiaz Hussain1, Atif Akbar2, Muhammad Asif1, Laraib Nisar3, Muhammad Naeem Ashiq3 and Furhan Iqbal1*

...) or high dose (50mg/ ml saline/ Kg body weight) of NiO NPs (average particle size 43 nm) for 14 days. Control groups were treated with saline solution. A series of behavioral tests were conducted in all subjects. Both low and high dose of NiO NPs treated male mice, during rota rod test, spent significantly less time on rotating rod than controls. Male mice treated with both doses of NiO NPs performed more stretch attend ref...

Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5

...98 of CXCL10 for all the samples. The results showed significant association (OR, 2.1 (0.99 to 4.30), p = 0.04) of A allele of rs2276886 with EPTB group as compared to the control. In genetic model analysis, we found strong association (OR, 0.4 (0.181 to 0.94), p = 0.03) of GG genotype with male with EPTB as compared to female in gender stratified dominant model. Furthermore, in context of different ethnic groups of Pakistani subpopulation, we found Punjabi sp...
Mutassim M. Abdelrahman1, Ibrahim Alhidary1, Mohsen Alobre1, Riyadh S. Aljumaah1 and Rifat Ullah Khan2*
...ent levels of artificial saliva (AS) on the rumen pH, epithelial tissues pigmentation, fecal odor and status of some trace minerals. Forty-five growing lambs were used in an 84-day trial. Lambs were randomly assigned to five dietary treatments as follows: TMR, AS1 (TMR +15 kg AS/Ton; 22.5 g/lamb/day), AS2 (TMR + 30 kg AS/Ton; 45 g/lamb/day), AS3 (TMR + 45 kg AS/ Ton; 67.5 g/lamb/day) and AS4 (TMR +90 kg AS/Ton; 135 g/lamb/day). The growth performance was not a...

Qi Zhuo, Yuanchun Yao, Meisongzhu Yang*, Jinhua Chen and Miao Tian

...ast cancer cells. Tissue samples (28) were collected, and malignant and normal tissues were confirmed pathologically. The breast cancer cell line MDAMB-231 and the immortalized normal breast epithelial cell line MCF-10A were used in this study. Western blot analysis of miR-216b-5p and HDAC8 protein expression in tissue samples; reverse transcription quantitative PCR (RT-qPCR) real-time analysis of miR-216b-5p, HDAC8 mRNA exp...

Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3 

...well as peripheral blood samples of cats with MG tumours was used. Blood samples and surgical material obtained from healthy cats after a planned hysterovariectomy were used as control. In cats with established diagnosis of stage II breast cancer (BC; n = 4), fibrocystic mastopathy (FCM; n = 3) and healthy cats (n = 4), the following were examined: (1) haematological parameters; (2) biochemical blood indicators; (3) expressi...

Abdulkhaliq A. Al-Janabi1*, Mohammad S. Alsalami1, Arkan B. Mohammed1, Abdulkhaliq A.R. Al-Douri2 

...se (AST) and alanine transaminase (ALT), were significantly decreased in both Omega-3 treated groups compared to the control. In conclusion, the supplement with Omega-3 (150 and 300 µl) induced the growth rate, and liver enzymes, and reduced their lipid profiles, suggesting it would be a beneficial dietary supplement for rabbits.

Keywords | Omega-3, Polyunsaturated fatty acids, Dietary supplement, Albino rabbit...

Heba S.S. Salem1, Hend M. Megahed2, Marwa M. Sarhan2, Maha M. El Alem3, Gehan N. Alagmy3* 

...nce of S. aureus as a causative agent. S. aureus was diagnosed in 40% of the examined cases via traditional culture methods and molecular confirmation using PCR. All tested S. aureus isolates were positive for virulence-associated genes (nuc, icaA, and Hlg). In an experimental trial, a total of 75 healthy rabbits were divided equally into five groups (n =15/each group). The first group served as a negative control, the second group was injected intra-articula...

Muhammad Jamil1*, Muhammad Hussnain Babar2, Aamir Hussain3, Rana Abdul Hameed Khan3, Hammad Hussnain4 and Saghir Ahmad4

...ix sites viz; Multan, Faisalabad, Khanpur, Bahawalpur, Vehari and Sahiwal in the Punjab province during Kharif 2020-21. The main objectives of the study were to quantifythe GEI component of variation and to assess the yield stability of recently bred upland cotton strains across test environments. Twenty-one cotton strains were sown according to a randomized complete block design (RCBD) with three replications. Additive main effects and multiplicative interact...

Auzair Javaid Butt, Irfan Ullah*, Shahid Ali, Khurram Nawaz Saddozai and Jahangir Khan

...y producers, twenty wholesalers and twenty retailers are randomly selected and interviewed for data collection. From the current research it can be revealed that majority of potato growers followed the same marketing channel i.e. Producer → Wholesaler → Retailer → Consumer. The analysis shows that marketing-margins of potato growers, wholesa

Sarzamin Khan1, Abdul Jabbar Tanweer2, Rafiullah1, Ibrahimullah1, Ghulam Abbas3*, Jabbar Khan4, Muhammad Saeed Imran5, Asghar Ali Kamboh6 

...alworm scales in feed (basal diet). Group 2, 3 and 4 were fed ration with 1, 2 and 3 g/kg mealworm scales respectively incorporated in the basal feed. Feed intake, FCR (feed conversion ratio) and body weight gain were recorded on weekly basis. The use of mealworm scales at level of 3g/kg in feed significantly (P<0.05) decreased feed intake, increased weight gain and improved feed conversion rate (FCR) as compare...

Tarek A. Wrshana1, Yousry A. Dowidar1, Bahgat A. El-Fiky2, Ali M. El-Rify1, Walaa A. El-Sayed3, Basem M. Ahmed4* 

...strategies based on the usage of stem cells includes the cells either themselves or their secretions (secretome), which has recently shown ability to inhibit SARS-CoV2 replication in-vitro. In this study, MSCs were prepared from the bone marrow of native Egyptian Fayoumi chicken. The MSC with the surface marker CD105 (CD105+) were magnetically separated and infected with virulent Newcastle...

Mohamed Saleh*, S.E. Laban, G.Z. Mostafa, S.T. Moubarak 

...fection of nest bedding (sawdust). Paraformaldehyde powder was mixed manually with nest bedding (5 grams per nest), while potassium peroxy- monosulfate 0.5% based disinfectant was sprayed in each nest. Random samples were collected before and after treatment by 24-, 72- and 120-hours intervals and were tested for total bacterial, coliform, and fungal counts to evaluate the reduction percent achieved. The obtained results ill...

Desy Cahya Widianingrum1*, Himmatul Khasanah1, Melinda Erdya Krismaputri1, Susan Barbara Patricia Sembiring Meliala2 

...er supplemented with VCO saponification (P1); wafer supplemented with VCO protected by formaldehyde (P2); wafer without VCO (P3); and wafer supplemented with VCO mixed on indigofera (P4). Each treatments were using six replications and tested for their physical quality, rancidity, and rumen fermentation characteristics and in vitro digestibility. The data obtained were analyzed using Analysis of Variance (ANOVA) and further tested with an Honestly Significant ...

Iqtidar Hussain1*, Shakeel Ahmad Jatoi2, Muhammad Jawad Nazir1, Ehtesham Ul Haq1, Faheem Abbas1 and Muhammad Saqib Raza Shah1

...in cultivated oat (Avena sativa L.), maize (Zea mays L.), rice (Oryza sativa L.), pearl millet (Pennisetum glaucumL.), bread wheat (Triticum aestivum L.) and sorghum [Sorghum bicolor (L) Moench. Response of aqueous leaves extract was assessed through various agronomic and physiological parameters including number of plants emerged, leaves per plant, chlorophyll content, root and shoot length, plant fresh and dry biomass were...

Sajid Hanif1, Abdul Shakoor1,4*, Muhammad Farrukh Saleem1, Ifra Saleem2, Sajid Ali3, Muhammad Awais Ashraf4, Majid Nadeem4, Hira Shair4, Anwar ul Haq5, Rana Abdul Hamid Khan6 and Muhammad Amir Amin7

...ersity of Agriculture Faisalabad. Treatments viz., control (no stress imposition) and stress treatments viz., drought; heat and combination of drought + heat stress effects were imposed at anthesis. Three different levels of foliar-applied proline included water spray, 10, 20 and 30-mM concentrations. Combined heat and drought stress increased unfertile tillers, unfilled grains and abortive, chalky and opaque kernels, along with declines in chlorophyll and rel...

Ghulam Qadir1, Khalil Ahmed1*, Muhammad Ashfaq Anjum1, Quais Muhammad Affan2, Muhammad Zaighum Mushtaq3, Muhammad Amjad Qureshi4, Amar Iqbal Saqib1, Hafeezullah Rafa1, Abdul Wakeel1, Ghulam Shabir1, Muhammad Rizwan1, Muhammad Qaisar Nawaz1 and Muhammad Faisal Nawaz1

...s for the utilization of salts affected soils is the screening of available local plants which can grow or survive under salt stress and have considerable economic importance to the farming community. Therefore, a three-years pot experiment was executed to explore the salinity tolerance of medicinal plants i.e., Podeena (Mentha spicata), Hina (Lawsonia inermis), Qulfa (portulace oleracea),...

Muhammad Umer Chattha1, Muhammad Ilyas2, Imran Khan1, Athar Mahmood1, Muhammad Bilal Chattha3*, Ambreen Fatima4, Muhammad Iqbal5, Muhammad Tahir Akbar6, Muhammad Mahmood Iqbal5, Faran Muhammad7, Muhammad Talha Aslam1 and Muhammad Umair Hassan1

...d of different levels of salinity stress i.e., 0, 8 and 12 dsm-1 and different chickpea cultivars i.e., NIAB-2016, Bittle-2016 and Bhakar-2011. The maximum time to 50% emergence (T50), and mean emergence time (MET) and minimum germination percentage (GP) and emergence index (EI) was recorded when high level of salt stress (12 dsm-1) was imposed, while minimum, T50, and MET and maximum GGP and EI was observed under control co...

Muhammad Mansoor1, Shahid Hameed Khan Khalil2*, Zafar Islam2, Muhammad Asif2, Ghani Akbar2, Muhammad Ashraf Khan3 and Ibadullah Jan4

...Kg dry dates whereas the same quantity of Rutab fruits on ripening and drying yields ¾ Kg of fresh dates. General evaluation of bunches bagging treatments on fruiting traits was conducted. It was concluded that bunches covered with blue paper bags obtained highest score among all the traits and is recommended to achieve best results under current climate change scenario. Generally, fruit covering shields the fruit from adverse effects of monsoon rains, ...

Ghalib Ayaz Kachelo1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Shahbaz Talib Sahi1, Nasir Ahmad Khan1, Akhtar Hameed2, Noor Muhammad1 and Muhammad Saqib Mushtaq1

...(25.910), garlic (Allium sativum) (28.087), neem (Azadirachta indica) (29.010) and aloevera (Aloe barbadensis miller) (29.538) mm as compared to control plates, while in chemicals Tilt showed minimum fungal growth (7.982) followed by Score (10.865), Antracol (11.395), Radomil Gold (11.965) and Blue Copper (14.390) mm as compared to control. In the greenhouse, the most effective fungicide and plant extract were applied as tilt and moringa alone and their combin...

Sohail Ahmed Talpur1, Imran Khatri1*, Maqsood Anwar Rustamani1 and Zubair Ahmed2

..., 1874 and Chaetocnema pusaensis (Maulik, 1926). Distributioanl map for each species is also provided.

...

Farah Rasheed1*, Ana Aslam1, Muhammad Aftab1, Ghulam Sarwar2, Raheela Naz1, Hina Nazir3, Sadia Sultana1, Amina Kalsom1, Nisa Mukhtar1, Ifra Saleem1, Qudsia Nazir1, Muhammad Arfan-ul-Haq1, Abid Niaz4, Muhammad Arif1, Aamer Sattar1, Sarfraz Hussain5 and Adnan Rafique6

...e grains remained within safe limit in all treatments but lower grain phytic acid have been observed where only recommended dose of NPK fertilizers were used.

...

Amna Sulaman1, Qurat-ul-Ain Ijaz1, Muhammad Shafi2 and Faiz Muhammad1*

...nt resource as well as a sacred animal. The meat of this animal is used in traditional medicine. T. pyrum is a less known species from Pakistani marine waters. This research validated its molecular identification as T. pyrum. The neighbor-joining results revealed that it clustered with the same species of family Turbinellidae except for the individual of the same species from India’s...

Shailendra Sharma, Smriti Shrestha, Naresh Pandey and Laxman Khanal*

...through the focal animal sampling method for 101 hours, and human disturbances were categorized into four levels based on the number and behavior of visitors. Out of the entire activity budget, resting (49.27%) constituted the highest proportion, followed by feeding (22.93%) and grooming (12.59%). There was a negative association between the grooming behavior and the number of visitors; however, no significant difference was observed in grooming proportion bef...

Ngoc Tan Nguyen1*, Pham Kim Ngan Nguyen1, Thao Huong Phan1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Cong Dinh Nguyen3, Nguyen Khang Duong4 

...mtDNA). Thirty-one blood samples that were collected from three native buffalo populations such as Bao Yen (BY: 7 samples), LangBiang (LB: 7 samples) and Thanh Chuong (TC: 7 samples) and two imported buffaloes from Thailand (T: 5 samples) and India (Murrah – M: 5 samples) were ...

Eman H. Abotalp, Sahar R. Mohamed, Jakeen K. El Jakee

...ypt are colonized with unsafe E. coli serotypes and the antibiotic resistance in these E. coli isolates. A total of 129 rectal swabs were gathered from apparent healthy and diarrheic canines and felines. By using Vitek2 compact system, 42 E. coli isolates (32.6%) were resulted from canines (24%) and felines (44.4%) rectal swabs. E. coli were serotyped to: O8, O25, O26, O28, O36, O55, O78, O86, O111, O114, O125, O127, O128 and O157. The resistance to 16 an...

Husmaini1*, Sabrina1, Firda Arlina1, Linda Suhartati1, Yozella Martilova2 

...um and Pediococcus pentasaceus using a purple sweet potato carrier on laying hen’s performance and egg quality. This study used 210 layers of medium-type laying hens, Strain Isa Brown, 38 weeks old. The method used was an experiment with a completely randomized design (CRD) with seven treatments and three replications. The observed variables were ration consumption, ration conversion, egg mass production, hen day prod...

Sajid Ali1*, Shahen Shah2, Muhammad Amin3, Asad Ali Khan2, Sajjad Khan4, Dawood Ahmad5, Faiq Ahmad2, Maaz Khan2, Sikandar Azam1, Siddique Ahmad2 and Bismillah Khan2

... Harichand, district Charsadda- Pakistan. Two levels of P (90 and 120 kg ha-1) and three levels of Zn (0 i.e., water spray only, 2.5 and 5 kg ha-1) were applied at three application stages (AS) i.e., full at vegetative stage V6 (AS1), full at reproductive stage R2 (AS2) and ½ at V6 and ½ at R2 stage (AS3) along with an overall control were used in the experiment. Randomized complete block design was used with three replications. Most of the studi...

Shahid Ali1*, Murtaza2, Waqas Ahmad1, Nargis Bibi3, Aftab Khan2 and Jahangir Khan

...Pakhtunkhwa, Pakistan. A sample of 300 rice growers was drawn randomly using a multistage sampling procedure through a well-organized pretested interview schedule. Stochastic frontier models (SFM-1 and SFM-2) were utilized to investigate effects of farm inputs and socioeconomic factors on rice production. Results of SFM-1 explicated that all the regressors except irrigation numbers were highly significant with the estimated ...

Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari

...h meal) and maintain the same egg production as control.
 
Keywords | Commercial ration, Tofu dregs, Tenebrio molitor, Nutritional content, Quail egg production
...
Abdulaziz A. Alaqil1*, Mohammed I. Buhaya2
...-source of omega-3 polyunsaturated fatty acids, on egg production performance and egg yolk cholesterol content, blood hematology and biochemical composition of commercial laying hens. A total of 180 Hy-Line brown laying hens 36-week-old were randomly divided into four groups (9 replicates x 5 birds each). The four groups were fed a basal diet formulated to contain; 0, 2, 4 or 6% LO for six weeks. Results showed a significant...

Razaq Adekunle Animashahun1*, Gbenga Emmanuel Onibi2, Samuel Olanrewaju Aro2, Oghenerobor Benjamin Akpor3, Funmilayo Abimbola Okeniyi1, Olayinka Olubunmi Alabi1, Michael Babatunde Falana1, Ayoola John Shoyombo1, Samuel Oyewale Olawoye1 

...and microbe fermented cassava stumps using Aspergillus niger (ATCC 16404) strain in a solid substrate. The dried and milled cassava stumps were aseptically inoculated with Aspergillus niger (ATCC 16404) strain in a solid-state at substrates to water ratio 1.0:1.0 w/v and then incubated for 192 hours at room temperature. Samples were taken at 48 h interval for the proximate, minerals and anti-nutrients composition determinati...

Hassan Raza1, Muhammad Zeeshan Majeed1*, Muhammad Irfan Majeed2, Mujeeb-Ur-Rehman1 and Muhammad Asam Riaz1

...rget site resulting in unsatisfactory and short-term termite eradication along with environmental contaminations. This situation necessitates looking for more target-oriented and ecologically safer strategies such as baiting insecticides with some cellulose attractant. In this study, 5% technical grade fipronil was formulated as small beads (3.5 mm) made by a matrix composed of 65% maize cob powder (as source of natural cell...

Waqas Raza1*, Muhammad Usman Ghazanfar1, Muhammad Asif2, Ikram-ul-Haq3, Muhammad Zakria4 and Laith Khalil Tawfeeq Al-Ani5,6

...havior of pathogen. The usage of various growth media is providing a useful pattern of the growth that can be utilized in determining the best possible control of late blight disease.

...

Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy* 

...kest disinfectant at the same conditions. Moreover, the organic matter hindered bactericidal power of many commercial disinfectants such as quaternary ammonium compounds (QUATS), halogen releasing agents including iodine, chlorine and its compounds, but some were not affected, such as glutaraldehyde. Finally, the success of the disinfection process in different poultry farms mainly depends on the selection of suitable disinfectants.

Keywords | Disinfe...

Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1  

... A total of 350 random samples were collected from animals and hospital acquired infected patients. The recovered isolates were identified biochemically by vitek2 compact, confirmed by PCR and tested for sensitivity against varieties of antimicrobials. Three multi-drug resistant isolates carried ica from human abscess (A9), sputum of dog (A12), and cow mastitic milk (A13) were sequenced with other genome sequences retrieved from the GenBank. Ten

Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1 

...gives an apprise on the usage of garlic (Allium sativum) extract as an alternative additive for sperm preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental protocol was designed as four groups (Control, G1, G2 and G3). Buffalo-bulls’ semen were exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the ...

Ayesha Khan*, Zubair Ahmad Khan and Urooba Pervaiz

...htunkhwa (KP). Purposive sampling technique (sample taken on certain judgments about the population or researcher chooses who would be appropriate for the study) was employed to select three districts i.e. Peshawar, Mardan and Swabi from Agro-ecological Zone C of KP. The population of the study comprised of all extension agents of the selected district. Data was collected through a well-developed interview schedule from all ...

Shomaila Sikandar1*, Imran Afzal1 and Sadaf Sarfraz2

...of these fungal genera Fusarium spp., Penicillium spp., and Aspergillus spp., are major mycotoxins producers in food and feed crops. Mycotoxins thrive in high-temperature, humid environments, and they can enter the food chain either directly or indirectly by contaminating food and feed crops. They can cause infection before and after agricultural crop harvesting. Economically mycotoxins infection leads to loss of feedstock, reduced livestock production, human ...

Gudeta Nepir Gurmu, Tade Bitima Mulisa, Alemu Lenco Gemechu, Kegna Gadisa Amena and Gemechu Nedi Terfa*

...stify;">Field pea (Pisum sativum L.) is one of the leguminous crops that is rich in protein and essential amino acids. A field experiment was conducted during the 2019 cropping season consisting of eight (Bursa, Burkitu, Adi, Herena, Hortu, Letu, T/shaman, and Weyib) improved field pea varietiesand one local variety at west Showa zone Oromia region to identify high yielding varieties. The ...
Irfan Shahzad Sheikh1, Syed Haseeb Shah1, Niamatullah Kakar2*, Mohammad Masood Tariq1, Muhammad Essa Kakar3 and Muhammad Zahid Mustafa1
...e growth and can be used safely at sub-therapeutic levels in food and/or water of broiler chicken. The result further determined that the longer use (6 weeks) of AGPs had no harmful effect on blood, liver and kidney in broiler chicken and can be used after withdrawal period.

...

Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid

...ingle layer of pregranulosa follicular cells and they remain dormant in the meiotic prophase I stage. Primordial pregranulosa follicular cells initiate activation of primordial follicle and govern the development of dormant oocytes. The primordial follicles take about 6 months to grow and develop to ovulatory graafian follicles in cattle and humans. Growth of preantral follicles is gonadotropin-independent whereas growth of ...

Muhammad Waseem Akhtar1*, Khalid Mahmood Khawar2, Muhammad Naeem Akhtar3, Muhammad Rafique1, Khalid Hussain4, Sharmin Ashraf1 and Muhammad Fida Hassan1

...l Research Institute, Faisalabad. 1 kg of each genotype was taken in plastic jars. There were three replications of each genotype for each insect. Twenty adults of dhora beetle were discharged in each container which were enclosed with muslin cloth with rubber bands. The Jars were placed at room temperature. Data of egg laying were observed after five days of release of insects. Data of emergence F1 adults were observed after 30 days of storage. At this time p...

Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3

...ce. A total of 1013 milk samples belonging to 726 cattle and 287 buffaloes were examined to detect clinical mastitis (CM) and subclinical mastitis (SCM). Mastitic milk samples (n=541) were further used to investigate the prevalence of bacteria associated with mastitis and their antimicrobial susceptibilities by standard disc diffusion assay. The results showed that the prevalence of CM and...

Maher Mohammed Yousef Alobaysi,1,4 Turki Albacker2, Waleed Alharbi2, Osama Almogbel2, Sadik Mohammed3, Fahad Al-Abbasi4, Vikas Kumar5 and Firoz Anwar4

...opin and Vit D in paired sample correlation. WBC counts measured before and after intervention were 5.81±1.12 and 5.19±0.98 respectively, which showed a significant fall in the WBC count and increase in cortisol after intervention. These alternations suggest to review and redesign the curricular or assigning duties to medicos under training.

...

Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3

Shou-Dong Zhang1,2,3, Dao-Xin Liu1,2, Dan Mou1, Tong-Zuo Zhang2, Jian-Ping Su2 and Jiu-Xiang Xie1*

...nutrients and avoiding disadvantageous secondary plant metabolites.

...

Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1

...eptor (ESR1) gene. Blood samples in this regard were collected from clinically diagnosed eighteen breast cancer patients, all possessing invasive ductal carcinoma. DNA was amplified through PCR for 2 different sequences of ESR1 gene. Twelve normal individuals of different ages were also characterized. Amplified products were digested with Xbal and Pvull restriction enzymes and were electrophoresed to visualize single nucleotide polymorphisms (SNPs); rs9340799 ...
Ghulam Nabi Dahiri1, Akeel Ahmed Memon2, Qudratullah Kalwar3*, Asmatullah Kaka2, Waseem Ali Vistro4, Yar Muhammad Jalbani3, Kiran Nazish3 and Jai Parkash Kolhi5
... treated with 2ml normal saline on day 0, 7 and 9 of the treatment. Group C was biostimulated and A was not biostimulated. The results showed that estrus response (60 %) and pregnancy rate (50%) was higher, onset of estrus was earlier (54.89 ± 1.20), duration of estrus was long (23.17 ± 1.20) in group D as compare to other groups. It was concluded in the present study that the biostimulation with ovsynch protocol can effectively be used to induce...

Jun Wang*, Xiaoming Jiang and Zhiwei Sun

...Kessler, 1876); Triplophysa siluroides (Herzenstein, 1888); Silurus lanzhouensis (Chen, 1977); Silurus asotus (Linnaeus, 1758); Channa argus (Cantor, 1842). The b values of the LWRs ranged from 2.598 for Ctenopharyngodon idella to 3.271 for Parabramis pekinensis. This study represents the first report on LRWs of commercial fish species in the whole mainstream of the Yellow River.The LRWs of five species have not been previously record. These data can serve as ...

Lei Xu1, Qin Yang2 and XiaoYan Zhang3,*

...e-linked immunosorbent assay to measure the levels of fibroblast growth factor-23 (FGF-23), 25(OH)D and sclerosin in the serum of patients. The levels of sclerosin and FGF-23 in the serum of patients in the experiment group were much higher than those in the control group, while the level of 25(OH)D was lower (P < 0.05). Aggravation of calcification came up with the increases in the levels of sclerosin, FGF-23, intact parathyroid hormone (iPTH), phosphorus,...

Amoon Danial1, Shahan Azeem1*, Sohail Raza1 and Muhammad Hassan Mushtaq2

...prevalence in the cattle sampled from Lahore and Rawalpindi districts of the Punjab Province of Pakistan. The objectives of the present study included evaluating the seroprevalence of BoHV-1 in goats accompanied by cattle in the areas where seropositive cattle have been previously identified. A total of 126 blood samples were collected from goats located in areas of Lahore District where previously cattle were seropositive f...

Indyah Aryani 1,3*, Suyadi Suyadi2, Hartutik Hartutik2, Kuswati Kuswati2 

...ndonesia. A total of 400 samples of Madura cattle were collected from four sub-district at Pamekasan Regency as follows: Pakong, Pasean, Batumarmar, and Waru (PAPABARU). The collected feces were examined using whitlock sedimentation methods. The specific species was identified by their morphology. A total 43 Madura cattles were infected by nematode worm as follows: Oesophagostomum sp., Cooperia sp., Capilaria sp., Ostertagia...

Muhammad Rabiul Hasan1, Zannatul Mawa1, Muhammad Ashekur Rahman1, Selina Yeasmin2, Yahia Mahmud2 and Muhammad Yeamin Hossain1*

...wo mussels (Anadara granosa and Meretrix lamarckii) in the Bay of Bengal, Bangladesh were investigated based on occasional data from January to December 2020. Samples were collected from the sampling sites (Sathkhira and Cox’sBazar) with the help of laborer in the presence of Principal investigator and research assistant. This study also revealed the maximum recorded length of A. granosa...

Sajjad Ali1*, Atta ur Rahman1 and Sher Ali2

...ucture. Built-up is necessary; however, it is worth mentioning to feed 11.23 million people in the district. The region’s land is the most fertile and productive in the Khyber Pakhtunkhwa province. Data were taken from the Agricultural Bureau of Statistics from 1990 to 2020, and the satellite imageries of the region were acquired from the United States Geological Survey (USGS). ARIMA technique is employed to get the fo...
Muhammad Younas1*, Khalid Hussain1, Abdul Ghaffar1, Muhammad Atiq2, Niaz Hussain1, Wasim Abbas3, Muhammad Azeem Khan4, Muhammad Nadeem1, Muhammad Irshad1, Nasir Ahmad Khan2 and Muhammad Zubair5
...ollowed by Mirpurkhas, Nasarpuri, HON-1069, Pania, Rubi F2, GSL-132, Red Moon, PK-1032, F-1122, Red Imposta, Early Red, Desi Large and Vrio-4 which showed moderately resistant response to A.porri with PDI of 11-20%, whereas, the four accession including Golden Arab, Desired, Sultan F1 and Red Nasik expressed Moderately resistant response with PDI of 21-40%. Moreover, six accessions (Yellow Gystal, Vrio-9, Vrio-5, Vrio-4, Vrio-1, and Vrio-3) expressed susceptib...

Muhammad Halim Natsir*, Osfar Sjofjan, Yuli Frita Nuningtyas, Danung Nur Adli, Lizar Basnelda, Dimas Frisky Apriyanto, Wahyu Tri Utami 

...ts consisted of B1T0 = basal diet, B1T1 = basal diet + 0.4% avocado seed flour, B1T2 = basal diet + 0.8% avocado seed flour, B1T3 = basal diet + 1.2% avocado seed flour, B2T0 = basal diet, B2T1 = basal diet + 0.4% avocado seed flour enhanced with nanotechnology, B2T2 = ba

Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1* 

...n in oocysts and comet assay were used to investigate the C-Oo.Nc destructive effects. The infectivity of sporulated oocysts exposed to LC50 & LC90 were evaluated by In vivo inoculation of broiler chicks. The results demonstrated that the increase in the rate of sporulation inhibition is directly related to the increase in C-Oo.Nc concentrations and exposure time. Analyzing the DNA damage in the oocysts exposed to different concentrations using comet as

Olufemi Bolarin, Sijuade Adebukola Adebayo and Sola Emmanuel Komolafe*

...y (180) yam farmers were sampled for the study. Primary data was gathered with the use of interview schedule. Descriptive statistics and regression were used to analyze data collected. The result shows that the mean age of the farmers was 45.3 years and 83.3% were males. The result also shows that weather forecast (Mean=1.42) and change in farming type (crop- livestock Mean=1.36) were not commonly used by farmers meanwhile the farmers preferred planting of ear...

Zulqarnain Haider1*, Muhammad Akhter2, Syed Sultan Ali1, Tahir Latif1, Rana Ahsan Raza Khan1, Awais Riaz1, Tahira Bibi1, Muhammad Ijaz3, Qasim Raza1, Mohsin Ali Raza4, Samina Sarfaraz1, Muhammad Iqbal1 and Muhammad Rafiq1

...lite basmati rice (Oryza sativa L.) lines including three checks were investigated in advance generation. Triplicated randomized complete block design (RCBD) was used during three consecutive years from 2016 to 2018 and the data were subjected to combined ANOVA, correlation, principal component, GGE biplots in order to demonstrate the stability and performance of these advance Basmati lines. A highly significant and exploitable level of genetic variability was...

Onwusiribe Ndubuisi Chigozirim*, Okpokiri Chibuzo Ikechukwu, Agu-Aguiyi Fortune Nneka and Oteh Ogbonnaya Ukeh

... practices. A multistage sampling technique was applied in choosing the 270 smallholder farmers in the studied area. Focused group discussions, interview sessions and surveys were used to obtain the required data. For the data analysis, descriptive statistics and correlation analysis were used to analyze the data. The findings revealed that the socio-economic status of rural farmers, especially income and education are significant factors influencing their acc...

Sidrah Ashfaq1, Muhammad Nadeem1*, Muhammad Yamin1, Talha Afzal1, Muhammad Waqar Akram1, Rabia Anam1 and Ali Mehboob2

...t. To overcome the above-said problems, there is a need to look for a feasible and robust solution for thermal applications in remote areas. Biomass gasification proved to be one of the most efficient and clean waste-to-energy conversion processes in contrast to direct combustion of agricultural waste. Other than controlled burning of biomass, it can be subjected for different purposes like a thermal, synthesis of ethanol, engine running, and for fuel cell app...

Muhammad Shafiq1, Tehseen Ashraf2*, Sehrish Mushtaq1, Naveeda Anjum3, Muhammad Asim4, Muhammad Aqeel Feroze3, Malik Abdul Rehman4 and Marja Aziz3

...iods were studied. GFP assays revealed that the putative transgenic calli were not transformed and died after 40-60 days. The experiment was repeated ten times to see the success rate of the regeneration system. In this study a protocol for chili plant regeneration was developed for callus induction, plant regeneration, and plant transformation. Because developing a viable chilli tissue culture and plant regeneration system is difficult, this study was designe...

N. Wachida1*, P. M. Dawuda2,1, I. U. Ate3,1, P. I. Rekwot4, 2 

...e-Linked Immunosorbent Assay (ELISA). Blood samples for serum were collected for oestradiol and luteinizing hormones. Follicular diameters at 42 hr were longer (P<0.05) in hot dry season (8.088±0.52 mm) than in raining seasons (8.618±0.9 mm) and cold dry season (6.338± 0.68 mm). At 72 hr follicular diameters were longer (P<0.0...

Al-Hassan M. Mostafa1*, Gehan Mohammed Sayed2 

...w. (fourth group). Fecal samples were collected weekly after 3 weeks post infection for Fecal Egg Count (FEC). One animal of each group was slaughtered at 7th week of experiment for sampling of abomasal specimens for histopathological examination and tissue estimation of Total peroxide, malondialdehyde (MDA), protein carbonyl (PC), Catalase, reduced glutathione (GSH) and superoxide dismuta...

Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2  

...se and mortality is necessary. The current study evaluated the efficacy of different NDV vaccines used in poultry flocks in Egypt: live attenuated NDV vaccines (genotype II) and live recombinant herpes virus of turkey (rHVT-ND-IBD) alone or in combination with inactivated NDV vaccines either of genotypes II or VII (commercially available or autogenously prepared). Different vaccination regimes are applied at various designated days to 260 commercial broiler ch...

Rijumoni Daimari, Silistina Narzari, Jatin Sarmah* 

...testine of Doom pig of Assam, India. The samples were collected from semi-extensively reared pig farms of Kokrajhar district, Assam. The approximate composition range of these edible viscera was found to be: moisture 50.67-68.47%, fat 0.47-9.85%, ash 0.16-0.92%, protein 6.85-22.36%, carbohydrate 10.18-24.65% and calorie 124.31-198.16 k/cal/100g. Among the sa...

Muhammad Jamil1*, Ghulam Sarwar2, Imran Akhtar3, Ghayour Ahmad4 and Saeed Ahmad4

Marcos E. Bollido1,2*, Renell Jay G. Villaluz1,2 and Ronald L. Orale1

...creased by 9-39% for the same year in review. It was noted that 27% of the supply was locally produced, and 73% were imported from other regions. Locally sourced pig supply is pegged at 53%, and the rest (47%) are imported from various towns in Samar and other regions. The demand for pork and live pigs is 27% and 55% higher than the supply. The said gap can be filled in by local farmers/raisers with an overall market opportu...

H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1 

.... A total number of 2160 samples included litter, pen litter, stored feed, feed from the feeders, water, drinkers, droppings, dust, swabs from walls, birds’ cloaca, worker’s hands, and wheels of vehicles. The results showed that there was a negative relationship between the prevalence of Enterobacteriaceae and the hygienic measures enforced in the poultry farms under study. The highest prevalence of Salmonella and E. coli was recorded in ...

Shandana* and Ayesha Khan

...cted to investigate the usage of ICT tools in agricultural extension services in Khyber Pakhtunkhwa. For the study three districts (i.e. Swat, Haripur and Mardan) of Khyber Pakhtunkhwa were chosen and a total sample size of 400 respondents was selected. Data were collected through pre-tested and validated interview schedule and analyzed using Statistical Package for Social Sciences (SPSS). The findings of the study showed th...

Hafiz Kashif Ali1, Iftikhar Ahmad1, Mujahid Ali2*, Zahoor Hussain3, Muhammad Ather Nadeem4, Malik Abdul Rehman5, Barkat Ali6, Muhammad Iftikhar7

...ayed by bio-stimulants (Isabion and Tecamin Max) with three different concentrations (1 ppm, 3 ppm, and 5 ppm) with the different number of sprays (1, 2, or 3 sprays). The first spray was applied after one month of transplanting, while the other two were applied at fortnight intervals after the first spray. Fisher’s Analysis of variance revealed that Isabion at 1 ppm as 1 spray, 3 ppm as 3 sprays, and 5 ppm as 3 sprays...

Riaz Hussain1*, Adnan Ihsan2, Azaz Ali Shah2, Najeeb Ullah2, Hamza Iftikhar3 and Ranra Jalal4

...ests of green pea (Pisum sativum L.) and their effect on yield/cost benefit ratio. The randomized complete block design (RCBD) was followed with 9 treatments and a control each replicated thrice. Treatments including the extracts of garlic bulb (Allium sativum) @ 2%, tobacco dry leaves (Nicotiana tabacum) @ 2%, red chili (Capsicum spp.) @ 5% and Chinaberry dry fruits (Melia azedarach) @ 10% concentrations were used singly an...

Paulus Klau Tahuk*, Oktovianus Rafael Nahak, Gerson Frans Bira 

.../DM) were relatively the same between treatment. Digestibility (%) of DM, OM, CP, CF and energy were relatively the same between treatment. The T3 treatment showed higher daily body weight gain (ADG) (P<0.05) with optimal feed conversion and efficiency than T1 and T2 treatment. Therefore, it can be concluded that the use of fish meal levels of 12% in a complete feed improves the growth performance of fattened male Bali ca...

Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3 

..., we concluded that the usage of a half-dose combination of enrofloxacin and amoxicillin is safer and more efficient in the treatment of Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens than the usage of these antibiotics in a full-dose combination or solely.

Keywords | Amoxicillin, Enrofloxacin, E. coli, Salmonella, Mixed infection. 

...

Asim Ali Khan1, Muhammad Ahmad Zeshan1, Yasir Iftikhar1*, Mustansar Mubeen1, Maham Sohail1, Sonum Bashir1, Malik Abdul Rehman2 and Muhammad Usman Ghani3

...za virus (CTV) expresses salient symptoms like stem pitting, yellowing of leaves, stunted growth of large, small lateral branches and twigs. The study was carried out to quantify the spatial and temporal distribution of CTV disease in major citrus growing areas of Sargodha district (Sargodha, Kot Momin, and Bhalwal). The samples from the declined orchards were collected based on symptomology and confirmed through ELI

Andi Hiroyuki1,2,*, Refina Sastya Qomariyuti1, Najmah Nur Islami3, Vanessa Ayu Sumirat4, Neni Anggraeni5, Mas Rizky A.A Syamsunarno2,6 

...fter that, the mice were sacrificed and blood samples were taken for hematological examination and the heart, liver, white fat tissue, and kidneys were weighed. The results showed that the average heart weight (0.120 grams) and white fat tissue (0.145 grams) decreased in the KSO group, as did RBC (8.285×106/μl), WBC (2,943×103/μl) and platelets (506 ,5×103/μl), but statistically there was no signif...

Shakeel Ahmad1, Muhammad Israr2*, Rasheed Ahmed3, Anam Ashraf1, Muhammad Amin4 and Nafees Ahmad5

...ed significantly. At the same time, savannas, permanent wetlands, urban and built-up lands, natural vegetation, permanent snow and ice, and barren lands increased substantially over the entire period. Due to the lack of baseline data, the LULC map will play an essential role in the sustainable management of LULC in the Hindu Kush, Karakoram and Himalayan regions of Pakistan. For the sustainable maintenance of the mountainous...

Naheed Zahra1*, Saira Batool1, Mubbashira Nazir1, Ghulam Akbar Malik2 and Iffat Batool3

...n: justify;">It is universally recognized that women are playing valuable role in all segments of crop and livestock; but unfortunately, the contribution of women in agriculture sector is still underestimated. The purpose of this study was to investigate women’s involvement in farming and livestock in Pothohar, particularly in rain-fed areas of Punjab, Pakistan. The research was carried out at Thatti Gujran, one of Fateh Jhang’s villages during 201...

Sajjad Khan, Fahad Karim Awan*, Zulfiqar Ali Gurmani, Muhammad Usama Hameed, Allah Bakhsh and Kainat Bibi

...ntal constraints, mainly salt and water stress due to their elevated degree of impression and non-availability of salt and drought tolerant oat cultivars. For the purpose in-vitro study was conducted at NARC fodder laboratory to study the response of two oat cultivars namely NARC and PARC Oat against drought and salinity stress. Salt concentration of 80 mmolLˉ¹ showed non-significant...

Ejaz Ul Haq, Urooba Pervaiz*, Muhammad Zafarullah Khan and Ayesha Khan

...a were collected from 60 sample respondents from three circles (Mattani, Peshawar and Tarnab) through pre-tested and well-structured interview schedule during February- March, 2020. The results showed that 48.3% farmers were motivated by extension staff towards tunnel technology, only 6.7% farmers got training regarding tunnel farming, 70% respondents reported that extension worker visited their field once a year. The study concluded that though extension work...

Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1* 

...nt study, 150 total meat samples, 100 from raw meat (beef=40, mutton=40, fish=20) and 50 from cooked meat (beef=20, mutton=20, fish=10) were randomly collected from district Hyderabad and cultured on different media for isolation of bacterial species. The isolated species were identified by different biochemical tests. The results showed that the contamination of bacterial organisms in both raw and cooked meat was highest in beef followed by mutton and fish re...

Dede Kardaya*, Ayu Ratnasari, Dewi Wahyuni 

...to people in Indonesia. Asam gelugur (Garcinia atroviridis) leaves are known to reduce fat content in duck meat because it contains hydroxy citric acid. This study aimed to examine the effect of Garcinia atroviridis leaf meal (GALM) supplementation in fermented nonconventional diets on the percentage of spent layer duck carcasses. The study used a completely randomized design with three treatments, and 50 replicates. The treatments consisted of 50% rice bran a...

Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti 

...Kernel Meal 

...

Bilal Dik1, Saima Naz2*, Muhammad Sohail Sajid3,4 

...aaetus pennatus, Aq. chrysaetos and Aq. gentilis. Circus cyaneus, Ci. macrourus, Clanga clanga and Accipiter brevipes were not found infested with the louse. There are some lice species reported for the first time in Turkey, put new records for the hosts and the country on record. Many Turkish birds are, however, yet to be screened for chewing louse infestation.

Keywords | Accipitriformes; Amblycera; Ischnocera; Phthiraptera; Turkey. 

...

Nurdan Urvaylioglu1*, Ogunc Meral1, Serkan Sayiner2, Ulvi Reha Fidanci1 and Arif Altintas1

...and subclinical mastitis samples. Somatic cell counts (SCC) in cows with clinical mastitis were significantly higher than the milk of cows with subclinical mastitis and healthy cows. Milk serum haptoglobin (Hp) and amyloid A levels were not determined statistically different between groups (p> 0.05). No correlation was found between CMT scores, SCC values, Hp and milk amyloid A levels in milk serum. There were no significant differences in Hp, MAA levels an...
Ahmed H. Massoud1, Mohamed S. Ahmed2, Moustafa Saad-Allah1, Aly S. Derbalah1, Ashraf Albrakati3* and Ehab Kotb Elmahallawy4*
... changes were nearly the same in all doses of the used pesticides. Taken together, our study recommends examining pesticides for their possible adverse effects on animals and humans in case of repeated use, even in small doses, before their application to agricultural fields.

...

Semine Dalga* and Kadir Aslan

...s prominently located dorsally at the intersection of the last premolar and the first molar in both sexes. The infraorbital foramen was located dorsal to the second upper premolar tooth on the os maxilla. The septal process was shown to terminate with two ends in females and a single end in males. It was observed that the processus nasalisi of the os inciivum extended to the os na

Muhammad Ikram Ullah

...erized by intellectual disability, facial dysmorphism, musculoskeletal and immune abnormalities. This study identifies a genetic mutation in a complex consanguineous Pakistani family of Punjabi language group. The family presented with rare complex neurological symptoms including mental retardation, facial dysmorphism, skeletal and muscle abnormalities. Five affected family members were recruited along with unaffected member...
Zain-ul-Aabdin Abro1*, Naheed Baloch1, Raza Muhammad Memon2, Niaz Hussain Khuhro2 and Iram Shaikh1
...zonata and Bactrocera dorsalis) (Diptera: Tephritidae) associated with mango and guava fruits were studied during (January, 2019 to December, 2019) to determine the population dynamics of these notorious pests in different climatic zones of Sindh Province. Steiner type (Tando Jam) traps baited with methyl eugenol and 5% pesticide were incorporated in the guava and mango orchards of Hyderabad and Larkana. Fruit flies were trapped in the field throughout the yea...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...in-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutio...

Zain-ul-Aabdin Abro1*, Naheed Baloch1, Raza Muhammad Memon2, Niaz Hussain Khuhro2 and Waseem Akbar Qazi2

...zonata and Bactrocera dorsalis are the deleterious pests of several plant species. Trybliographa daci (Weld.) and Diachasmimorpha longicaudata (Ashmead) are natural enemies of fruit flies widely used as bio-agents in biological control programs against Bactrocera species. In current investigations we inspected the infected guava and mango fruits from lower (Hyderabad) and upper (Larkana) Sindh regions for population surveillance of parasitoids during 2019. The...

Farheen Shaikh*, Saima Naz and Nadir Ali Birmani

...al mounting in Canada balsam. At present 130 specimens were recovered and classified into 4 species which belongs to 3 genera. The species and their prevalence are 19.23% for Menopon gallinae (Linnaeus, 1758), 23.84% for Menacanthus stramineus (Nitzsh, 1818), and 22. 30% for Menacanthus abdominalis (Piaget, 1880) which belongs to the family Menoponidae and 34.61% for Goniocotes gallinae (de Geer, 1776) which belongs to the family Philopteridae. All four specie...

Juweria Abid1, Shakeel Ahmad2, Humaira Wasila3, Tooba Asif4, Muhammad Farooq5* and Attaullah Jan6

...f KP and after composite sampling dried in oven and stored at room temperature for further biochemical analysis. Samples were then analyzed for minerals content, total phenolic compounds, total flavonoids and antioxidant activity. Physicochemical characteristics, proximate composition and energy density were also estimated. Statistical analysis showed significant differences (p <0.05). Fat content (6.73±0.06%) and total solid (15.21±0.01%) wer...

Shakeel Ahmad1*, Humaira Wasila2, Juweria Abid3, Nazir Muhammad4 and Hazrat Usman5 

...n different milk product samples were collected from 3 different markets in the study area to get a composite sample. AOAC 15th edition methods were used to analyze moisture, ash, carbohydrates, fats and protein contents of milk products. Atomic absorption spectroscopy and flame photometry were used for mineral analysis. Total phenolic compounds were evaluated by the Folin-Ciocalteu method. The aluminum chloride colorimetric...

Kanwal Hanif1, Dilbar Hussain1, Qurban Ali1, Asad Aslam1*, Tamsila Nazir1, Muhammad Saleem1, Muhammad Faheem Akhtar1, Muhammad Zubair2, Najuf Awais Anjum3 and Muhammad Umar Qasim1

...e Research Institute, Faisalabad. The viability of eggs was evaluated by exposing egg cards to insecticides by dipping them in insecticide solution for 10 seconds while adult survival was investigated through dipped surface residue bioassay method. The results revealed that new chemistry insecticides viz., Flubendiamide, Flonicamid and Chlorantraniliprole were comparatively safer to egg pa...

Kokab Nazim1*, Asghari Bano1 and Ghulam Jellani2

...es. Antioxidant enzyme essay showed enhanced production of Superoxide Dismutase and Catalase coupled with higher accumulation of proline in tolerant genotypes while their production was lower in sensitive genotypes during two growing seasons under stress. All the genotypes made two major clusters. Main cluster “I” was comprised of two genotypes. Main cluster “II” was comprised of 43 genotypes. Cluster “II” was divided into A...

Sonia Kanwal1, Muhammad Naeem1*, Zaib Un Nisa1, Rabia Farhat1 and Zia Ur Rehman2

...d lac III (n=10)]. Blood samples were collected during all production stages and serum has been preserved at -20 0C and used to evaluate the differences in vitamin E, vitamin C, Triiodothyronine (T3), Thyroxine (T4) and Cortisol. The statistical analysis was done by two way ANOVA and Duncan Multiple Range Test (DMRT). T3 and T4 showed significantly different results  during various stages of lactation in Lohi sheep and Beetal goats. However, Vitamin E, Vi...

Ghulam Qadar* and Khalid Nawab

...mmu and Kashmir by using sampling technique and well-structured interview schedule of 5-point likert scale was prepared in light of the study objectives. In addition, SPSS software V. 20.0 was used to analyze the research data. Principal Component Analysis (PCA) test was conducted to overcome the multidimensionality and multicollinearity issues in the data to produce valuable components. Results showed that among teaching competencies, 15 variables were analyz...

Khalid Hussain1, Muhammad Younas1*, Niaz Hussain1, Abdul Ghaffar1, Muhammad Raheel2, Anees Akhtar3, Muhammad Irshad1, Munir Abbas1 and Farah Shabir4

...e to the prevalence of Fusarium wilt. Present investigations were conducted at Arid Zone Research Institute, Bhakkar, Punjab, Pakistan during winter 2021. Experiment was laid out in Randomized Complete Block Design (RCBD) following three replications. Thirty chickpea genotypes were examined for their resistant levels against Fusarium wilt caused by Fusarium oxysporum ciceris (FOC). Six exh...

Yue Ren1, Ting Jia2, Hao Zhang1, Zhengkun Wang1 and Wanlong Zhu1*

... and Lijiang. Using microsatellite approaches, we observed considerable genetic variation and high heterozygosity in T. belangeri chinensis populations in different Yunnan province, and Chuxiong population and Lijiang population had relative low genetic diversity. Moreover, skull morphology changed for adapting to changing environment in T. belangeri chinensis. Finally, MengLa population had remarkable differences with the other populations both in genetic div...

Nurcan Karslioglu Kara*

...t significant, it can be said that the same interval is advantageous for low disease incidence in Simmental cows. Furthermore, it can be accepted that the same interval is optimum also for the best lactation milk yield since it was not found a significant relationship among the average milk yields according to AFC. In conclusion, it can be said that plan...

Muhammad Usman1*, Aneela Zameer Durrani1, Nasir Mehmood2, Muhammad Hassan Saleem1 and Mamoona Chaudhry3

...1 tick pools using PCR assay and sequencing in the Lahore City Metropolitan Area, Pakistan. Potential association of various risk factors with occurrence of Lyme borreliosis was also estimated via univariate and multivariate logistic regression. From each dog, blood and tick samples were collected and then tick species were identified. DNA extraction was followed by detection of 16S rRNA signature gene using B. burgdorferi s...

Wael Mohamed Wafa1*, Mohammed Mahmoud Hegazy1, Mohamed Mohamed El-said Ibrahim1, Hamdy Abdala El-Nagar1, Rehab Fawzy Ismail2 

...ght. All groups were fed same diet; control group was untreated (CG), while those in the 2nd and 3rd groups were orally treated with 300 ml GL/head/twice/week (GG) or daily 25 g PF/100 LBW (PFG) from 14 to 18 month of age. Results showed that PFG achieved the highest (P<0.05) body weights, weight gains, and body measurements at puberty, 1st service, and conception. Values of red and white blood cells, hemoglobin, packed cell volume, plasma total proteins, g...

Hossam Mahrous Ebeid1, Ahmed Abdelkader Aboamer1*, Amgad Ahmed Abu Elella2, Ibrahim Mohamed Khattab3, Osama Hefny Matloup1, Fatma Ibrahim Hadhoud1  

...nd ADF for DMSC using in sacco technique. Fifteen lactating Damascus goats (averaging 45.4± 0.5 kg, body weight and averaging milk yield 900±65 g/head/day) were randomly assigned to one of three treatments for 6 weeks to study DMSC’s effect on blood metabolites and quality and quantity of the produced milk. Treatment groups were provided either a control diet or control diet with 40 g/head/day (DMSC40) or 80 g/head/day (DMSC80) of DMSC. DMS...

Shad Mahfuz1,2†, Hong-Seok Mun1,3†, Muhammad Ammar Dilawar1, Keiven Mark B Ampode1,4, Veasna Chem1, Young-Hwa Kim5, Jong-Pil Moon6, Chul-Ju Yang1,7* 

...y 59.13 kWh/head and the saving efficacy was about 49.6% than the conventional incubator. Internal temperature was higher (P < 0.05) in solar based incubator. The ammonia concentration and hydrogen sulfide concentration were significantly lower (P < 0.05) in solar based incubator than the control incubator. The solar based heating incubator might be eco-friendly and renewable source of energy for the sustainable pig production.

 

Key...

Shabana Ehsan1*, Aneela Riaz2, Muhammad Amjad Qureshi1, Abid Ali1, Ifra Saleem3, Muhammad Aftab3, Khalid Mehmood4, Fakhar Mujeeb1, Muhammad Asif Ali1, Hina Javed1, Fraza Ijaz1, Anwar-ul-Haq5, Khaliq-ur-Rehman3 and M. Usman Saleem

...l micronutrient, is necessary for human health and to maintain the integrity and development of the plant. In Fe-limiting conditions, plants and plant growth-promoting rhizobacterial (PGPR) have a siderophore production mechanism. Inoculation with seed soaking of such siderophore-producing bacteria can be a cost-effective biofortification technique. The current study includes the collection of rhizobacterial isolates from wheat, maize, sorghum, millet, and mai...

Khalid Khan1*, Mahmood-ul-Hassan2, Ihsan Ullah1, Haris Khurshid1, F.Y Saleem Marwat1, Salman Saleem1, M. Jehanzeb1 and Zubair Ahmad1

...he world; now it is necessary to understand and utilizing the genetic diversity of sorghum hybrids for better crop production. In this study, crossing and selfing was done in parental lines of sorghum hybrids; such as A, B and R line system out of which 14 hybrids were finalized based on its morphological traits and statistically interpreted. Its genetic diversity is most important for exploitation of the genetic resources to develop promising hybrids. The obj...

Pharaoh C. Sianangama1*, Brian Tembo2, Sylvia J. Harrison1, Rubaijaniza Abigaba1,3 

...n-AIH). Free-catch urine samples, from 18 randomly selected dairy animals, were collected on day 15, 22, 30, and 35 after insemination and used at a dilution rate of 1:4. Maize seeds were soaked in different treatment groups, followed by observation for germination and shoot length. Data were analysed descriptively to obtain the means, and inferential statistics including Kruskal-Wallis and Bonferroni tests were employed to explore significance between the mea...

Syed Ishfaq Ali Shah1,3, Azaz Ahmad2 and Wanzhi Cai1,*

... Acanthaspis quinquespinosa (Fabricius, 1781), is a common species in Pakistan. Due to its variable color patterns, its taxonomic distinction from Acanthaspis flavipes Stål, 1855 has been confounded. The genitalia in Reduviidae are commonly used for species identification and in present studies, because of identical genitalia, A. flavipes Stål, 1855 stat. restit. is restored as a junior synonym of A. quinquespinosa

Muhammad Umar1*, Mubashar Hussain1 and David C. Lee2

...> 500 m apart at each sampling point in all three study sites. We conducted both morning (0500-0800 hours) and afternoon (1600-1900 hours) surveys, recording all birds seen or heard along transects to a maximum perpendicular distance of 50 m; 0.1 km2 surveyed per transect. In total, we recorded 39 bird species belonging to 20 families and eight orders. We found significant differences in seasonal abundance and species richness related to species’ resi...

Zahin Anjum1*, Mubashra Tarana1, Shaista Ali1, Faryal Yousaf1, Amina Rahat1 and Sumbla Yousaf2

...sh were carried out. The samples were obtained from five different sites of the University Campus, Peshawar- Pakistan. The Atomic Absorption Spectrometry was employed for determination of Cu, Zn, Pb, Ni, Ca and Mg. Furthermore, a spectrophotometer was also used for the purpose of phosphorus (P). However, the analysis of sodium (Na) and potassium (K) content was done through a flame photometer. It was observed that gizzard had the highest concentration of Zn (2...

Ahmad Nadeem1,2 and Rubina Arshad1,2,*

...morphology on De Man Rogosa Sharpe (MRS) agar plates, Gram staining, biochemical tests and molecular characterization. The co-culturing of LAB isolates with fungal cultures (in vitro) showed that among seven LAB isolates, only three possessed antifungal activity. One promising wild type isolate (LPA6) was subjected to gamma irradiation for mutation induction. Seeds of two chickpea varieties were inoculated with LAB isolates in four treatments comprising of T1 ...
Azra Kalhoro1, Abdul Aziz Mirani2, Fozia Khan Siyal1, Tahira Jatt2*, Abdul Razak Mahar1, Sadia Iram3 and Muhammad Abbas Bhutto4
... of SW respectively. The samples analyzed through atomic absorption spectrophotometer using flame atomic absorption techniques revealed that among the five treatments, accumulation of the six metals was found higher with 100% SW, which was decreased with decrease in SW concentration up to 25% SW. The minimum accumulation of the metal was noted with 100% FW (control). Among the five types of vegetables, cabbage and cauliflower revealed a high tendency of accumu...

Ashira Manzoor1, Imran Ahmad Khan1,2*, Muqadas Sadiq3, Muhammad Omer Iqbal4 and Shaukat Hussain Munawar5

... rats. Group-1 was given saline for 2 weeks and on 11th day DOX 18mg/kg was given intraperitoneally. Group-2, Group-3, Group-4 rats pre-treated orally with the leaf extract of C. colocynthis 250mg/kg, 500mg/kg, 750mg/kg respectively for 14 successive days and on 11th-day DOX was inoculated, while group-5 was used as positive control and was given dexrazoxane 180mg/kg after 30 min DOX was given. The levels of CK-MB, LDH, troponin-I, AST, CRP, ALT, and ALP were ...

Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*

...growth. The clonogenic assay was performed to determine the clonogenic efficiency CPSCs. The expression of cell surface markers was detected by immunofluorescence, flow cytometry, and RT-PCR, and it was found that the CPSCs positively expressed FGFR-3, collagen type II, CD44, CD73, CD90, vimentin, Notch1, CD105, and CD106, but negatively expressed the haematopoietic stem cell surface marker CD34. Furthermore, CPSCs were successfully induced to differentiate in...

Sekobane Daniel Kolobe, Tlou Grace Manyelo*, Jones Wilfred Ng’ambi, Munyadziwa Felicia Dorcus Nemauluma, Emmanuel Malematja  

...W of both sexes were the same (P>0.05) across all treatments for the first 2 weeks. During the 3rd week, AKLM inclusion levels significantly (P<0.05) affected DM intake, FCR, GR, and LWs of both sexes. In the last 3 weeks of the trial, FI, FCR, GR, and LW for both male and female broilers were similar (P>0.05) across all treatments. AKLM did not affect (P>0.05) gut organ digesta pH, lengths, and weight values of male and female broilers aged 21 day...

Ibrahim S. Abu-Alya1*, Nagwa I. Sheraiba2, Enas K. Aziz2, Noha A. Osman2, Afaf A. Kishta1 

...up) nourished with the basal diet only, T2 given the basal diet supplemented with 0.5% garlic oil, T3 given the basal diet supplemented with 1% garlic oil, T4 given the basal diet supplemented with 0.5% ginger oil, T5 given the basal diet supplemented with 1% ginger oil, and T6 given the basa

Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2 

... the infected mice. Most samples of mice expressed a different level of tissue necrosis where anti-caspase-3 was examined. Additionally, several mast cells in the mice liver samples were observed. By reestablishing efficient immune responses and preventing evasion mechanisms of the parasite, these findings help improve the understanding of local immune responses to CE and maybe lead to the design of novel treatments.

Qamar Javed Iqbal1*, Zainab Masood2, Asad Abbas3 and Sidra Munir4

...etic acid to ethylenic unsaturated double bond were studied. It was found that maximum Relative epoxy yield (REY) was 68% at temperature 60oC (333 K) with molar ratio of Double bond: Acetic Acid: Hydrogen peroxide (D: A: H) 1.0:0.5:1.5 at stirring speed of 1000 rpm. The kinetics of epoxide formation was studied under different ranges of temperatures i.e. 50oC, 60oC and 70oC.The Activation Energy of reaction was found to be 23.64kj/mol with the help of Arrheniu...

Ahmad-ur-Rahman Saljoqi, Muhammad Tayeb and Muhammad Salim*

...unkhaw-15, PS-15, NIFA-Insaf-15, NIFA-Aman-15, Pakistan-13, PS-13, Shahkaar-13, PS-5, NIFA-lalma were planted in Randomized Complete Block design (RCBD) having three (3) replications. Data regarding the population trends of R. padi and its associated natural enemies were recorded on weekly basis. The result showed significant differences among different wheat cultivars with respect to the time interval for most of the parameters. Aphid’s population was a...
Rafiq Ali Khan1, Sajjad Ahmed1, Salim Raza2* and Shehla Gul1
...tics. The representative samples of the units are used for Unconfined Compressive Strength (UCS) tests to establish the fracture pressure and fracture gradient of the formation in Kohat-Potwar and Nizampur Sub-Basins and adjoining areas. Based on the UCS tests and extrapolation techniques, a benchmark value for fracture gradient of Lockhart Formation is derived that is 0.91 psi/ft. This value is very important parameter in drilling operations and can be used i...

Marat Kuibagarov1, Assylbek Zhylkibayev1, Dinara Kamalova1, Anara Ryskeldina1, Nurdina Yerzhanova1, Yerlan Ramankulov1, Alexandr Shevtsov1*, John A Angelos2  

...y pathogenic factor necessary for attachment of M. bovis to the surface of the eye epithelium. To evaluate pilA diversity amongst Kazakh isolates of M. bovoculi, the pilA gene sequence was determined for 35 isolates of M. bovoculi. Ten genotypes (gNA1-gNA10) were identified which were 96.1% to 99.8% identical. It was found that as a result of the replacement of cytosine with thymine at the 97th position of the gNA8 genotype, a stop codon resulted which, if ex...

Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana  

...polymorphisms (SNP). The samples used in this study were blood samples (n= 70) collected from Pesisir cattle (aged 2-5 years). DNA was isolated and the targeted region was amplified using the polymerase chain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used in this study was polymorphic. There were 5 polymorphisms in the exon 9 and intron 9 re...

Siriporn Namted, Kanokporn Poungpong, Wiriya Loongyai, Choawit Rakangthong, Chaiyapoom Bunchasak* 

...eir fractions, and the dosage as feed additive on the growth performance, immune function, and gut morphology of pigs. Inconsistent results have been reported for the various yeast products utilized in the animal feed industry, with differing types of YE processing (autolysis or hydrolysis) and differing doses/responses. In a feed additive, the components of the cell wall (β-glucan and mannan-oligosaccharides) and some ...

Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim* 

...olate. So concluded that safety measures should be done because the disease has zoonotic effect on public health. The prevalence of Fascioliasis was high in Qualyobia, Egypt, a lot of liver was condemned, which resulted in financial losses for affected farmers. In order to control the disease and raise farmer awareness, it is vital to implement the appropriate preventive measures.

Keywords | Fasciola species, Prevalence, Phylogenetic analysis, ITS2 gen...

Hamenya Mpemba1, Fan Yang1, Kirsty J. MacLeod2,3, Dusu Wen1, Yan Liu1,4 and Guangshun Jiang1,*

...as perceived as risky to safer spaces where predation risk is lower, which can have important consequences for investment in foraging, movement, and mating, and for the behaviour and habitat use of other species, such as mesopredators. These changes in prey and mesopredator behaviours are likely mediated by the presence of predator cues in the environment. Here, we test how different predator cues (visual and odor) from familiar and novel predators (brown bear...

Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1 

...xemption. Milk is a necessary social meal, but as the quality and quantity of milk decline, it has an impact on dairy farmers’ livelihoods and family nutrition, resulting in significant losses. Because the quantity and quality of milk are so important in the dairy sector, there have been significant financial losses. As a result, adequate maintenance and preventative measures must be maintained in order to ensure any dairy business is viable and sustaina...

Shad Mahfuz1,2†, Hong-Seok Mun1,3†, Veasna Chem1, Keiven Mark B Ampode1,4, Muhammad Ammar Dilawar1,5, Hae-Rang Park1, Il-Byung Chung1, Young-Hwa Kim6, Chul-Ju Yang1,5* 

...r. Therefore, it is necessary to identify the effective repellent for pigs. For this test, 6 pigs (Avg. wt. 66.03± 3.99 kg) were housed in an individual pen containing repellent and without repellent feeder. In this current study, a total six (6) ingredients (capsaicin, red chili, ground garlic, dead insects, denatonium benzoate, and thiophanate-methyl) were used as repellents. The repellent ingredients were used to h...

Syeda Batool Zehra1, Abdullah G. Arijo2, Aly Khan3, Nasira Khatoon1* and Samina Waheed1

...se enterobiasis, under unsanitary conditions, the Oxyruidae family causes health concerns, particularly in children. Samples were collected from the laboratory of Jinnah Hospital, Karachi, Sindh, Pakistan. The data of 60 pinworm-infected children were recorded. These children were of various sexes and ages. Pinworm prevalence in children by gender from 2019 to 2020 demonstrates that females have a higher rate of incidence than males because of un

Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2

...to yellowish mottling, mosaic spots and deformation were observed in leaves of fig plants. To evaluate the presence of Fig latent virus (FLV -1) in fig plants. One hundreds fig trees were collected with virus-like symptoms and symptomless fig trees. The virus was detected by DAS-ELISA. Infected fig leaves were mechanically inoculated on Chenopodium amaranticolor L. and reinoculated on Ch. ...

Reza Aghnoum*, Hamid-Reza Sharifi, Masoud Ghodsi and Ahmad Zare Fizabadi

...erm experiments are necessary to determine the capacity of non-pathogenic nematodes in suppression of soil-borne pathogens including the fungal and pathogenic nematodes.

...

S. M. Ashraful Karim1, Md. Shohel Al Faruk2* 

...pected of CKD. The Blood sample was collected for estimation of biochemical parameters e.g. ALP, ALT, AST, Phosphorus, Glucose, Total protein, BUN, and Serum creatinine. The Urine sample was taken also for determination of Urine pH, Specific gravity, Proteinuria, and Glucose. Ultrasonography was performed to check the condition of the kidney. Increased levels of BUN, Serum creatinine, Proteinuria, and thickened cortex of the...

Ayesha Saeed1*, Farzana Kishwar2, Ahsan Nazir3, Sharjeel Abid4, Muhammad Abiodullah5, and Danial Ali6

...nature. Traditionally, Musa Acuminata (MA) and Hibiscus Rosa Sinensis (HRS) leaves famous for its medicinal value as they contain phytochemicals such as triterpenoids, polyphenols, flavonoids, and alkaloids. Keeping in view and get benefit from their fruitful properties the aim of the study was to develop antibacterial dry sheet nanofiber mask loaded with leaf extracts of MA and HRS as antibacterial agents in

Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1* 

...aloes. Forty five paired samples of ovarian tissue from buffaloes were obtained from slaughterhouse. Ovaries were collected and CLs were classified morphologically into three stages: early, mid and late stages. Moreover, the CLs were frozen at -80 °C for NO estimation in CLs homogenate, RNA isolation and gene expression. Blood samples were obtained for P4 serum levels estimation. Results showed a significant rise of TNF&...

Nishim Bhusal1, Bhuwan Raj Bhatt2*, Saroj Shrestha3 and Arjun Chapagain4

...otal of 270 fresh faecal samples were collected purposively from the study area with different ages, sex, stage, and breeds for examination (sedimentation method) to visualize eggs of Fasciola microscopically. The obtained data were coded and analysed using Microsoft Excel 2016. The overall prevalence of fasciolosis in cattle was found to be 15.56%. Age and sex-wise prevalence was found to be statistically significant (P<0.05), while stage and breed-wise pr...

Muhammad Tariq Navid1,2*, Mian Muhammad Awais2*, Muhammad Irfan Anwar2 and Masood Akhtar2

...ryngeal and cloacal swab samples were collected in sterile containers. About 13.61% of the samples were found seropositive by using commercially available ELISA kit. The supernatants from oro-pharyngeal and cloacal swabs of the seropositive samples were separated and divided into two segments; one was used directly to ...

Muhammad Izhar ul Haque1, Farhan Anwar Khan1*, Umar Sadique1, Hamayun Khan1, Zia ur Rehman1, Salman Khan1, Hayatullah Khan1,2, Faisal Ahmad1,3, Mumtazur Rahman1, Faiz Ur Rehman1, Muhammad Saeed1, Mehboob Ali1 and Saqib Nawaz1

...eshawar. Serum and fecal samples were collected at random both from commercial farms and abattoirs. Additionally, tissue samples (intestine and mesenteric lymph node (MLN)) were collected at random from sheep and goats at abattoirs of district Peshawar. Analyses of serum samples by iELISA revealed the presence of antib...

Murad Ali Rahat1*, Muhammad Israr2, Hakim Khan1, Ishtiaq Hassan1, Mohammad Islam1 and Muzafar Shah3

...human eye color from DNA samples. To check the validity of IrisPlex system in the local Pakhtun population living in Shangla Valley of Pakistan, the current study was carried out. DNA samples as well as photographs were taken from total of 226 individuals. Exclusion and inclusion criteria were set for samples collection. The analysis showed that brown eye color individuals were greater in ...

Saleh M. Albarrak1*, Fahad S. Aldahmashi1,2, Abdulrhman A. Alrubayan1,2, Abdel Kader A. Zaki1,3 

... assay to specifically detect and quantify ASAs in the serum and seminal plasma of 29 infertile dromedary male camels, which were of two different breeds (Waddah & Majaheem) and three different ages (> 3 to ≤5, ≥5 to ≤7, and >7 years). ASAs were detected in ≥ 80% of the exami...

Shahid Amin1*, Jabbar Khan1, Imran Khan2, Dost Muhammad1, Kamran Ullah Khan1, Naqeeb Ullah Khan1, Muhammad Azhar Jameel3 

...atus. Total of 500 blood samples (Sheep, n=250, Goat, n=250) were collected from different areas of South Waziristan. Samples were processed meant for the incidence of haemo-parasites and diverse hematological limitations were anticipated. Prevalence of haemo-parasitic diseases was recorded as 14.4, 10.4 and 7.2% for anaplasmosis, theileriosis and babesiosis, respectively. Different risk factors were studied but statistical significant changes (p<0.05) were...

Cheng Zhao1,2, Qin Shi1*, Gen Yang3, Youyuan Tan2, Songwen Tan2, Jiahong Li2 and Marwan M.A. Rashed2

...was indicated that diet, safety and temperature were the main factors affecting the habitat use of Sichuan sika deer in winter.

...

Florence N. Nworah*, Nene O. Uchendu, Ifeoma F. Chukwuma*, Emmanuel C. Ossai and Emmanuel C. Ezeako

... Group A received normal saline, group B remained untreated, group C, D, E and F were respectively treated with standard haematopoietic drug, Ginovera (5 mg/kg body weight), 50 and 100 mg­­/kg body weight of raw blood and 100 mg­­/kg body weight of cooked blood respectively. Determination of the constituents of turtle blood revealed the presence of vitamins such as Vitamin A, B6, B9 and B12 and minerals such as Selenium, Iron and Zinc. After th...

Hira Jabbin1, Muhammad Arshad1*, Naunain Mehmood1, Wardah Hassan1, Syed Zakir Hussain Shah2 and Farzana Siddique3

...rishable. So, it is necessary to seek viable alternatives that help to preserve meat freshness and quality and to increase its shelf life. One of these alternatives is the chitosan which is natural preservative having antimicrobial properties. In present study, the effectiveness of chitosan coatings as natural preservative was assessed on rancidity development and quality changes in mori f...

Akbar Hayat1*, Ehsan Ul Haque1, Marayam Nasir2, Rab Nawaz3, Tariq Mahmood4 and Sohaib Afzaal1

Hasnain Raza1*, Tanveer-ul-Haq1, Muhammad Imran1, Nabeel Ahmad Ikram2 and Muhammad Bilal Shoukat1

...ater production and disposal have become issues of concern with the growing population and increase in overall water usage. About 4.36 billion m3 (BCM) of wastewater is generated every year in Pakistan and not even 1% of the total wastewater gets treated before its disposal. The reported research aims to assess the concentration of contaminants in wastewater. Samples of wastewater were col...

Madad Ali1, Muhammad Ahsan1, Muhammad Zubair Akram*2 and Samreen Nazeer3

...ersity of Agriculture Faisalabad to screen out the best single cross doubled haploid hybrids of maize using eleven doubled haploid single cross hybrids. A randomized complete block design was used to conduct the experiment with three replications under normal environmental conditions. Data for different growth, yield, and agro-morphological parameters were analyzed at a 5% probability of the HSD test. Significant differences were present among the doubled hapl...

Samina Kausar1, Rana Badar Aziz2, Muhammad Waseem3, Muhammad Ahmad3, Hamza Shafiq4, Muhammad Asim5, Usama Zia6, Sobia Afzal7, Wanpeng Xi8*, Mansoor Hameed1* and Muhammad Usman Shoukat9

...marigold flowers, and capsanthin and capsorubin in crimson pepper. Zeaxanthin is protective against scalp diseases, UV and skin redness. Lycopene is a bioactive component regarding the remedy of persistent sicknesses and lowering the chance of cardiovascular illnesses or cancer. The tremendous results of carotenoids in human food have prompted numerous efforts in plant genetic engineering to supply products with greater carotenoid accumulation, which isn&rsquo...

Ishtiaq Ahmad1*, Muhammad Nafees1, Maryam2, Irfan Ashraf3, Ambreen Maqsood4 and Muhammad Saqib5 

...tional losses. The fruit samples were collected from mulberry plants growing at the experimental area, The Islamia University of Bahawalpur, Pakistan. Analysis of variance showed significant effect of drying methods on fruit quality of mulberry (p ≤ 0.05). There was significant difference (p ≤ 0.05) in fresh and dry fruit weight, fruit width and area, and Cu contents of both the species in spectrophotometer. Reduction in fruit weight, fruit area and mois...

Adefunke Fadilat O. Ayinde1*, Peter Allison Johnston2, Olanrewaju Olusoji Olujimi3, Purnamita  Dasgupta4 and Dare  Akerele5

...sed the perception of cassava-based farmers to climate variability in two ecosystems in Nigeria. Climate data (spanning 1951 to 2010) were used to corroborate and evaluate the perceptions of farmers. Four hundred smallholder farmers were interviewed in Ogun State (rain-forest zone) and Kwara State (derived savannah) using a multi-stage sampling technique. Farmers perceived climate variabil...

Sanwal Masood1, Dildar Ahmed2, Silvia Machado1 and Sohaib Aslam1*

...the present study, water samples were collected and analyzed for selected physicochemical parameters including toxic metals for determining the impact of land uses on the ground water quality of Lahore, Pakistan. Our results showed that water of three sites namely Shadman, Samnabad and Township had the TDS above 300 mgL-1 and this value was comparatively greater than samples of other areas. Only one site named Ravi Town show...

Ty Nguyen, Giang Thi Huong Phung, Lan Le Thuy Hoang and Giang Van Tran*

...plified and sequenced 10 samples of CO1 gene of M. cunnesius sample in Tam Giang lagoon were recorded size of 650 bp. The average ratio of four types of nucleotides is A: 23.38%; T: 32.62%; G:18.15%; C: 25.85%. The rate of difference in CO1 sequences of the 2 samples is low compared to previous studies. The similarity coefficient of M. cunnesius in the two areas of Tam Giang lagoon is very...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

... or together on the reversal of hematological changes induced by carrageenan in the mice model. Mice were induced edema via λ-carrageenan (50µl/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophi...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

... or together on the reversal of hematological changes induced by carrageenan in the mice model. Mice were induced edema via λ-carrageenan (50µl/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophi...

Sherif Abdelghany1, Hossam Mahrous Ebeid2*, Ahmed Abdelkader Aboamer2, Mohamed Ali Radwan1, Rania Agamy1 

...tive feed staff and milk samples were collected and analyzed. Feeding practices efficiency indicators were calculated to be compared with standards. Results revealed that most farmers in the studied two districts used the concentrate to feed animals, which was formulated at home and mixed with roughage at feeding times. The Newly reclaimed district used mixed roughages (P < 0.001) compared with Nile Valley district that provided mainly wheat straw. Green fo...

Humera Manzoor1,2,5 Norbert Brüggemann2,3, Hafiz Muhammad Jafar Hussain1, Tobias Bäumer2, Frauke Hinrichs2, Muhammad Wajid4, Alexander Münchau2, Katja Lohmann2* and Sadaf Naz1*

...xt-align: justify;">A consanguineous Pakistani family with three affected siblings was investigated to determine the genetic diagnosis of an inherited contracture disorder. Whole-exome sequencing was performed for four participants. Variants were filtered based on homozygosity in the three patients and heterozygosity in the obligate carrier (mother), predicted effect of variants on the encoded protein, and their frequencies in public databases. Sanger sequenci...

Widiastuti Ardiansyah1,3, Osfar Sjofjan2*, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2 

...productive organs of Mojosari ducks. A total of 500 grams of turmeric were prepared for the identification of bioactive compounds and flavonoids, while a total of 280 Mojosari ducks aged 20–28 weeks were used in the second step. The study comprised a descriptive analysis and a completely randomized design with six treatments and four replications with eight Mojosari laying ducks in e...

Muhammad Shuaib1*, Abdul Hafeez1, Naila Chand1 and Muhammad Tahir2

...of soybean hull in the basal diet resulted in lower production performance and nutrient digestibility than the control group.

...

Masixole Maswana, Thinawanga Joseph Mugwabana and Thobela Louis Tyasi*

...ong donkey breeds of the same area within that country. One of the key aspects in animal evolution and migration from one area to another is at the core of it informed by adaptability. This study will help donkey farmers, extension officers and researchers to understand the morphological variation among donkey breeds.

...

Noha M. Abd El-Azeem*, Hemat A. Abdel Magied, Mervat N. Ghazal, Nehad A. Ramadan, Enayat H. Abo El-Azayem, Y. S. Hussein, Heba H. Habib 

...he treatments were fed basal diet as control (G1), basal diet plus 0.3 % turmeric (G2), 0.05 % MOS® (G3), 0.015 % Biostrong® 510 (G4), 0.05 % MOS +0.3 % turmeric (G5) and 0.05% MOS +0.015 % Biostrong (G6), respectively. Rabbits fed with turmeric, MOS and Biostrong recorded a significant (P≤0.05) increase in doe weight at mating, prepartum, partum and weaning period, and the rate of feed intake increased in the tre...

Irshad Ahmad1,2*

...l be limited with the passage of time due to the insubstantial resources available for wild fish harvesting. Therefore, other sources of feedstuffs need to be investigated to substitute FM and FO in aquafeeds. Terrestrial crops can be used to substitutes a portion of the FM however; they can result in changes in the nutritional quality of the fish produced. Microalgae can be considered as a favorable alternative that can substitute FM and FO ensuring the princ...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
Abdullah Iqbal1, Muhammad Abubakar2, Shumaila Manzoor3, Muhammad Kamran Ameen4 and Rani Faryal1*
 
...b> from serum samples. Antigen shedding by vaccinated animals via nasal and fecal route was checked by the haemagglutination test and RT-PCR. All group 2 bucks, developed humoral protection against PPR within the 1st week. Only, 50 percent bucks of group 1 developed humoral protection against PPR after one week of vaccination. The mean percent inhibition value of competition-ELI
Mansha Tahir1, Abdul Rauf2,3, Syed Ayaz Kazmi2,3*, Nuzhat Shafi2, Muhammad Hasnain Ali Gillani3, Sundus Khawaja4, Syeda Maria Fiaz Bukhari4, Tehniat Summer4 and Muhammad Majid Bashir2

Mingqing Wang1 and Xiaojuan Chen2,*

...lone. According to the dosage of glucocorticoid methylprednisolone, the patients were divided into three groups, each containing 60 patients. The changes of arterial blood gas parameters including pH value, oxygen partial pressure (PaO2) and carbon dioxide partial pressure (PaCO2) were compared. We found that respiratory stimulant combined with different doses of glucocorticoid methylprednisolone positively improve the pH value of patients with acidosis, incre...

Ayma Aftab, Samia Afzal* and Muhammad Idrees

...e result. Nasopharyngeal samples from a 22 years old man were detected as negative for COVID-19 for consecutive three RT-PCR tests. Complete blood count (CBC), D-dimer, serum ferritin, eosinophil sedimentation rate (ESR) test, tuberculosis test, real time PCR and high-resolution computed tomography (HRCT) were done to rule out the cause of flu like symptoms. HRCT reveals a haze area in the right perihilar region adjacent to medial part of horizontal fissure on...

Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2

...a, Punjab, Pakistan. The sample size was calculated using Raosoft sample size calculator. A total of 250 animals were sampled from herds of different sizes for sampling through convenient sampling technique. A questionnaire having variables related to animal species, age, herd size, parity, breeding method and housing ...

Asma Basharat1, Abdul Wajid2*, Andleeb Batool1, Tayyeba Batool3, Abdul Basit4, Kamran Abbas5, Aziz Ullah1 and Mahmood Shaukat6

...lyzed a total of 134 DNA samples including 51 CP clinically assessed patients and 83 healthy controls. Our preliminary results revealed two novel missense substitutions i.e. P180Q in T-box domain and S328I in C-terminal of TBX22, while unrelated healthy controls revealed no sequence variants in these locations. The present report is first of its kind in Pakistani population. The findings suggest that TBX22 polymorphisms may responsible for a significant propor...
Jie Wu1,2, Xiang-long Lu1,2, Wen-dan Wang1,2, Tian-you Wu1,2, Xing-yi Zhang1,2 and Yan-jing Su1,2*
...e fatty acids (FFA), the saturated fatty acids(SFA) content in milk C was lower, and unsaturated fatty acid (UFA) content was higher, than in milk E. The highest FFA contents were observed for C14:0, C16:0 and C18:1, and the C16:0 content in milk E was significantly higher than in milk C. In brief, although whey protein improves foaming, but the presence of FFAs strongly impairs foaming.

...

Anum Syyam1,2, Nazish Saqlain1, Sidra Hareem1, Naghmana Mazhar1, Liaqat Ali3 and Samia Afzal2*

...2019 to Feb, 2020. Blood samples were collected from 350 healthy and voluntary group “O” donors (males = 320 and females = 30, median age 27 years ± 6.4 SD). Estimation of IgM type anti-A and anti-B titers was done using the standard tube technique and the samples positive for hemolysis were further evaluated for titers of IgG type anti-A and anti-B. All the titers were confirmed microscopically. The total...

Aqsa Batool1, Masood Rabbani1*, Farhat Nazir Awan2, Sohail Raza1, Sehrish Firyal3 and Shahan Azeem1

...Province. A total of 400 samples were randomly collected from cattle and buffalo of wheat rice Punjab, mixed Punjab, cotton/wheat Punjab, Low intensity Punjab and Rain-fed Punjab zones. The study revealed individual seroprevalence of BoHV-1 49.5% in cattle and 51.5% in buffalo. Over all prevalence in five zones of Punjab was observed as 25% in mixed Punjab to 71.25% in Rain-fed Punjab. The BoHV-1 seropositivity ranged from 30 - 60% in cattle and 20-75% in buff...

Qingyue Han1, Ying Li1, Jichang Deng1, Kunxuan Huang1, Yanyang Yang1, Quanwei Li1, Zhuowei Zhang1, Na Qiao1, Yanju Ji2, Khalid Mehmood3, Sarfaraz Ali Fazlani4, Hui Zhang1 and Zhaoxin Tang1*

K. H. El-Kholy1*, Enayat Hassan Abo El-Azayem2, Safaa Ataya Barakat2, Mona Gamel Mohamed3, Sara H. M. Hassab1 

... First group was fed a basal diet only (control group, G1), while the other three groups fed a basal diet along with 0.5 (G2), 1.0 (G3) and 2.0 g (G4) AV leaves powder/kg diet. The results revealed that, AV addition at its highest levels (G3 and G4) significantly (P≤0.05) increased the final body weight, daily weight gain and feed efficiency. The AV inclusion, especially in high dose “G4”, lead to significant ...

Chattida Panprom1, Nakrob Pattanapon1, Wannisa Meepoo1, Soontaree Petchdee2* 

...gs with valve disease is safe and appropriate for preserving cardiovascular functions.

 

Keywords | Dog, General anesthesia, Mitral valve degeneration, Noncardiac surgery 

...

Faiq Ahmad1, Shahen Shah1*, Muhammad Amin2, Ikram Ullah3, Sajid Ali4, Maaz Khan1, Muhammad Shakur5 and Sajjad Khan6

...wing. These ratios compensated total of 120 kg N ha-1. Nitrogen application at the rate of 120 kg ha-1 compensated as 50% from FYM and 50% from urea without beneficial microbes resulted in delayed emergence (15.4 days) as compared to rest of treatments, while delayed heading (114.8 days), anthesis (124.7 days) and physiological maturity (158.9 days) were recorded with application of 120 kg N ha-1 applied as 50% from FYM and ...

Inam Ul Haq1*, Humara Umar1, Farah Umar2, Naeem Akhtar3 and Muhammad Jan4

... of true to type healthy saplings, there is a dire need to optimize the protocols for nursery production. In order to attain success in this sector, investigation was carried out to improve the rooting ability of a widely grown olive cv. “Arbequina”. This research aimed to enhance the rooting ability of semi-hardwood cuttings through application of different separate and combined concentrations of indole butyric acid (IBA), urea phosphate (UP) and ...

Zarnosh Habib1, Muhammad Ibrahim2*, Nasir Shah2, Israr Ullah3 and Norman Javed Gill4

... fruit fly Bactrocera dorsalis (Hendel) at various host pupae depths (0, 1, 2, 3, 4 and 5 cm) in plant debris and at different host pupae ages (24, 48, 72 and 96 h) in laboratory at 25 ± 2ºC, 60 ± 5% relative humidity and a photoperiod of DL 14:10 h. The observations indicated a significant effect of different depths of host pupae and its age on the amount of parasitism by parasitoid. Maximum significant (F = 10.78, P < 0.0001) parasitize...

Andy1,4*, Ratmawati Malaka2, Sri Purwanti3, Hikmah M Ali2, Tutik Lusyta Aulyani4, Danung Nur Adli5 

... of T0 = control, T1 = basal diet + acidal, T2 = basal diet + 0.5% encapsulation of liquid smoke, T3 = basal diet + 1.0% encapsulation of liquid smoke, T4 = basal diet + 1.5% encapsulation of liquid smoke. Results were analysed using general linear model using SAS Demand on Academics...

Ghani Akbar*, Zafar Islam, Muhammad Umar and Shahi Salam

...tudy evaluated the water saving and productivity benefits of rice on furrow irrigated narrow beds (NB-65cm furrow spacing with 2 crop rows), drip irrigated wide beds (WB-130cm furrow spacing with 5 crop rows) and flood irrigated flat basin (FB) as control. The irrigation was scheduled at above field capacity (30kPa matric potential). The calibrated Aqua Crop FAO model was used for evaluating the root zone and atmospheric water balance and water productivity ba...

Muhammad A. Ahmad1, Shabbir Hussain2*, Humera A. Awan3, Muhammad Riaz4 and Muhammad Saeed1

Bilal Saeed Khan1*, Muhammad Arslan1, Abdul Ghaffar2, Muhammad Farooq2, Saghir Ahmad3, Sami Ullah4 and Awais Rasool5

...898 soil inhabiting mite sample slides were prepared from soil collected samples and after microscopic observation of slide specimen, 08 different families were recognized as Laelapidae, Pachylaelapidae, Ameroseiidae, Macrochelidae, Parasitidae, Phytoseiidae, Uropodidae, and Bdellidae. Macrochelidae and Parasitidae were the most prevalent familities while Pachylaelapidae and Bdellidae were recorded in the lowest numbers. The...

Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3 

... out includes; purposive sampling and accidental sampling techniques with the main respondents, namely breeders, collecting merchants, wholesalers, retailers, and consumers. The number of respondents in this study was 135 persons. Methods of data analysis carried out include; qualitative analysis and quantitative analysis. Qualitative analysis is intended to find out the characteristics of...

Sugiharto Sugiharto*, Ikania Agusetyaningsih, Endang Widiastuti, Hanny Indrat Wahyuni, Turrini Yudiarti, Tri Agus Sartono 

...ted papaya seed and chitosan on growth and haematological parameters in broilers stocked in high-density pens from 15 to 28 days old. A total of 490 broiler chicks were distributed into four groups, including CONT (chicks receiving basal feed and raised under normal density [10 chicks per m2]), HSD (chicks receiving basal feed and raised under high density [20 chicks per m2]), HSD-P (chick...

Hams M.A. Mohamed1*, Katreen K.G.2, M.W. Abd Al-Azeem1, Faysal A. Wasel2, Ahmed M. Abd-Eldayem3 

... raw milk. In total, 150 samples of raw milk from vendors and nearby dairy farms in Sohag, Egypt, were collected. The samples underwent a bacteriological investigation. The results showed that 22 out of 150 samples had Listeria spp. contamination. Polymerase chain reaction (PCR) using the Listeria iap gene identified 13 of the 22 isolates that were reported as biochemically positive Lister...

Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2 

...d Simmental bull has the same quality of fresh semen, but Bali bull had better viability and abnormality compared to those Simmental breed of frozen semen.  

...

Abdul Kabir1, Muhammad Rasheed2, Hubdar Ali Kaleri5, Depeesh Kumar Bhuptani3, Mithan Kumar4, Raza Ali Mangi6, Abdul Wahid Solangi4, Sheva Dari1, Saqib Kakar4, Panah Munir4, Ekra Akbar4 and Rameez Raja Kaleri4,5*

Amber Khalid1*, Farah Ashfaq1, Shahzadi Farah1, Amna Akbar1, Farkhanda Manzoor1, Muhammad Babar Khawar2, Saira Riaz3 and Safina Ashfaq3

...t was carried out. Blood samples were taken and lipid Profile, serum creatinine and Serum albumin analysis was done by using automated chemistry analyzer. Results concluded that the experimental subjects have shown a significant difference (P<0.00) in HDL level as compared to control subjects. Result of t test has shown significant increase (P<0.00) in total cholesterol and triglycerides level. Results have shown that there is highly significant correlat...

Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*

... organs, left tibia were sampled for the quantity of phosphorus in plasma, its digestibility and tibial calcium and phosphorus. The present study reported that H group was found higher in FI, BWG and FCR as compared to other groups. Higher gizzard, liver and heart weight were recorded in group A and B while tibia ash, Ca and P in group H. Described results concluded that supplementing liquid phytase enhances breakdown of phytate phosphorus, phosphorus availabi...

Layth Saleem Salman Al-Shihmani

... study were determined , samples were taken from each horizon according to the specific depth of each horizon, and the laboratory examination was conducted for the physical and chemical properties. As for the mineral examination, it was conducted for only two depths (surface depth and subsurface depth). The results showed a variation in the chemical properties represented by the electrical conductivity values, pH of soil, organic matter, carbonate minerals, an...

Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassa

...ckens were offered the basal dietas Negative Control (NC, group 1); the other three groups 2, 3, and 4 were fed NC supplemented with 0.5% HSCAS, SC and HSCAS plus SC respectively, group 5 chicks were fed a basal diet contaminated with 1ppm AFB1 as Positive Control (PC), the other three groups 6, 7 and 8 were fed PC supplemented with 0.5% HSCAS, SC, and HSCAS plus SC respectively. Results exhibited that broiler chicks fed wit...

Muhammad Jamil1*, Rao Sohail Ahmad Khan2, Rana Abdul Hameed Khan3, Khezir Hayat4, Saeed Ahmad5, Abdul Sattar6, Umair Faheem7, Hira Saher8 and Ayesha Bibi8

...cations viz., Multan, Faisalabad, Khanpur, Vehari and Sahiwal in the Punjab province in Pakistan for two years 2020-21 and 2021-22. Ten promising upland cotton genotypes recently bred by different research stations were sown by following a randomized complete block design (RCBD) with three replications. The main objective was to pinpoint cotton genotypes with stable yield performance across the studied environments. Additive main effects and multiplicative int...

Rezqita Putri Pitaloka1, Kartiawati Alipin1, Mas Rizky A.A Syamsunarno2, Gemilang Lara Utama3, Ramdan Panigoro2, Ratu Safitri1* 

... plants. Sappan wood (Caesalpinia sappan L.) contains active compounds, such as brazilin and flavonoid with the ability to chelate Fe. This study aims to determine the effect of sappan wood ethanol extract (SWEE) as an adjuvant and substitute for iron chelator. The adjuvant test group was given 60 mg/kg BW iron dextran (ID), 75 mg/kg BW deferiprone (DFP), and 50 to 200 mg/kg BW SWEE, while...

Antonius1,3, Anuraga Jayanegara2*, Komang Gede Wiryawan2, Simon Petrus Ginting3, Anjas Asmara Samsudin4, Elizabeth Wina5 

...ch), specifically: T0 (basal diet without gambir supplementation), T1 (basal diet + 2 g/kg DM gambir extract), and T2 (basal diet + 4 g/kg DM gambir extract). Results revealed that addition of gambir extract did not alter the consumption of dry matter, organic matter, crude protein, crude fiber and crude fat. The T1 and T2 treatments resulted higher (P<0.05) average daily gain of 52.87%...
Humaira Gul1*, Muhammad Junaid Yousaf1*, Fawad Ali1, Mamoona Rauf1, Farhad Ali2 and Iqthedar Ali3
...rticularly, the ratio of sand and clay. The current research was carried out to investigate the effect of different ratios of sand and clay composition on growth and biochemical aspects of Barley (Hordeum vulgare L.) and variations in soil physical and chemical properties were tested. During the experiment, plants were grown in eleven sets of soil combinations texture wise with varying ratio of sa

Lopamudra Samantaray*, Yashaswi Nayak 

...tated research towards a safer natural alternative, such as the use of phytobiotic essential oils (EOs). The purpose of this study was to determine the effect of phytobiotic EOs on laying hen performance, physical characteristics, and egg cholesterol content. A total of 280 birds aged 70 days were enrolled in the study, which included seven experimental diets supplemented with EOs of black pepper, turmeric, and fennel at rates of 0%, 1%, and mixed diet at 0.5%...

Pham Tan Nha*, Le Thu Thuy  

... fermented feed to the basal diet. The trial lasted 9 weeks with Noi chickens from 6 to 14 weeks of age. The results showed when adding yeast feed at 4% (DM) to the diet of Noi chickens, there is an increase the weight and decrease in FCR of Noi chickens. Especially the PM treatments had the best results. At crude protein level, the CP20 treatments was better than the CP16 treatments. Microorganisms of chicken manure, especially the PM treatments was the high...

Chanathip Thammakarn1*, Sawanya Umphonphison1, Paitoon Kaewhom2, Kanokrat Srikijkasemwat1 

...021. A total 2,002 serum samples were collected from 16 farms located in 10 provinces and tested by Enzyme-Linked Immunosorbent Assay. Among total serum samples, 914 samples were further tested by polymerase chain reaction (PCR) to detect ORF2 gene. The results revealed that 81.17% of pigs had low positive level antibody against PCV2, while 10.29% had h...

Abdullah Al Musabbir1, Md Abedur Rahman2*, Naveed Anjum2, 3 and Mustajab Ali4,5

Agha Mushtaque Ahmed1*, Fahad Nazir Khoso1, Ali Zachi Abdulqader Alhilfi2, Sohail Ahmed Otho1, Qurban Ali3, Din Muhammad Soomro1 and Zubair Ahmed Soomro1

...erties and environmental safety. In this context, we evaluated the insecticidal effect of four botanical oils against C. maculatus including neem seeds (Azadirachta indica A. Juss), rocket seeds (Erucas sativa Mill.), cotton seeds (Gossypium hirsutum L.) and mustard seeds (Brassica nigra L.). The effect of these oils was recorded on insect mortality, number of adult insects, seed damage (punctured seeds and weight loss of ch...

Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1 

...s. In the present study, samples were collected from 100 Egyptian domestic ducks of different ages from seven Egyptian governorates from period 2018 to 2020. Tissue specimens were allocated for HPAI H5N8 diagnosis using rRT-PCR and the results were 66 positive samples with the highest incidence in the Giza governorate. The winter season showed the highest incidence with 28 positive samples...

Ifrah Amjad1, Muhammad Nouman Khalid1*, Muhammad Kashif1, Muhammad Noman1, Sajid Ali2, Rizwan Ahmed Shaikh3, Muhammad Babar4, Muhammad Asim Bhutta5 and Amna Bibi5

...e and Biology (NIAB), Faisalabad. A pot experiment was also performed to study the influence of complete submergence on elongation and survival percentage of fiveSub1 genotypes along with two high yielding local cultivars. All Sub1 genotypes showed less elongation percentages than Super Basmati and KSK-133 while maximum survival percentage was observed in IR-07-F289 Sub1 followed by Swarna Sub1 and FR-13A. Five rice genotypes (FR-13A, Swarna Sub1, Ciherang Sub...

Emtiaz Ibrahim Ghoniem, Gaber Mamdouh Abdelgalil, Amira Farouk Gad* and El-Sayed Hassan Eshra

 
...te garden snail, Theba pisana is one of the widely distributed gastropods and is a dangerous agricultural pest for many plants. Copper sulfate (CuSO4) is extensively used for controlling a number of molluscs in many areas. In this study, CuSO4 toxicity indices against T. pisana after 24, 48 and 72 h using the topical application technique were estimated. Additionally, in vivo evaluation of acetylcholinesterase (AChE), alkali...

Shama Sadaf1*, Komal Hassan1, Ayesha Saeed1 and Zeeshan Ahmad2 

...ed 100% reduction on all samples after 22 hours, but after six days only one colony of microorganism was appeared on treated fabric, while nine colonies was observed on untreated cotton sample The cotton fabric treated with B. monosperma and L. chinensis exhibited 100% reduction after 22 hours and even after six days interval. In case of mechanical properties (Tensile strength, Tear strength) antimicrobial finish had made po...

Muhammad Khalid Bhatti, Khalil Ahmed*, Ghulam Shabir, Muhammad Irfan, Muhammad Ashfaq Anjum, Muhammad Sarfraz, Amar Iqbal Saqib, Abdul Wakeel, Hafeezullah Rafa, Nadeem Iqbal, Muhammad Qaisar Nawaz, Ghulam Qadir, Muhammad Rizwan and Muhammad Faisal Nawaz

...nsidered one of the most salt sensitive cereals, however, a great genetic diversity exists within rice species for salinity tolerance. Therefore, a study was executed to identify salinity tolerance of seventeen advance rice lines based on of agronomic characters and Na and K contents. Advance lines of rice namely, SRI-22, SRI-23, SRI-24, SRI-25, SRI-26, SRI-27, SRI-28, SRI-29, SRI-30, SRI-...

Hafiz Husnain Nawaz1*, Ayesha Ahsan2, Amir Afzal1, Muhammad Ashraf Sumrah1, Muhammad Jan1, Kashif Ali1, Muhammad Arsalan1 and Rizwan Latif3

...lign: justify;">Cannabis sativa is a high-value crop that grows naturally in Pakistan’s highlands. This is commonly known “Bhang” in Pakistan. This study is an attempt to recognize the C. sativa plant’s wonderful physiochemical qualities and socioeconomic benefits. Antioxidants abound in C. sativa extract, thanks to its high phenolic content. It’s a high-polyu...

Md. Aminul Islam1*, SM Mostafizur Rahaman Sumon1, ANM Aminoor Rahman2, Fahima Khatun3 

...er. A total of 85 faecal samples from five different dairy farms were examined. The overall infection rate of helminths in dairy cows is 37.65%. A total of five different types of nematodes were observed in all the investigated dairy farms. The highest infection rate (11.76%) and the lowest infection rate (4.71%) was found for Neoascaris spp. (Toxocara spp.) and Fasciola spp., respectively. Hemonchus spp. were fully (100%) susceptible to Ivermectin, Fenbendazo...

Ahmed M. El-Waziry1,2*, Saeid M. Basmaeil1, Ibrahim A. Alhidary1, Gamaleldin M. Suliman1,3, Mutassim M. Abdelrahman1, Maged A. Al-Garadi1 

...d the concentration of unsaturated fatty acids and conjugated linoleic acid (CLA). The benefits of these acids with respect to human health include lower cholesterol and blood pressure and a reduced incidence of diabetes. There have been few studies on the use of ionophores in the diet of goats. Therefore, more research are needed concerning the influence of ionophores on ruminal fermentation, performance and characteristics of carcass of goats.

Keywor...

Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
...tification of biological samples found in mass disasters and criminal events by using the 6-dye GlobalFilerTM amplification kit with 21 autosomal STR loci in our laboratory for validation purpose and routine use. In this regard, swab samples from different regions of Turkey (n=350) were collected and studied. The validation of the GlobalFilerTM Amplification Kit was c...
Ahmad Zaman, Azhar Rafique*, Farhat Jabeen and Tayyaba Sultana
...rved from seven selected sampling sites. There was a highly significant difference (P<0.01) in the diversity and abundance of open field area and built-up area. All 87 species and 203304 individuals were observed from open field area while built up area harbor only 55 species and 64709 individual birds. Among sampling sites, agriculture land was the most dominant site in terms of number of individuals while highest avian ...

Riaz Noor Panhwar1*, Abdul Fatah Soomro1, Muhammad Chohan1, Illahi Bux Bhatti1, Ali Hassan Mari1, Samia Arain1 and Sagheer Ahmad2

...ted work regarding appraisal of variation in lately evolved sugarcane genotypes for morphological and quality-related characters has been reported in Pakistan. Evaluation of sugarcane genotypes for desired attributes acceptable to growers and sugar mills is necessary before releasing them as commercial varieties. The study was undertaken to evaluate variations in sugarcane genotypes employing four morphological (Cane thickne...

Khunsa Khakwani1*, Muhammad Asif1, Zaheer Ahmed2, Ghulam Sarwar1, Yasmeen Abbassi2 and Ghulam Murtaza2 

Andi Tenri Bau Astuti Mahmud1, Lellah Rahim2, Muhammad Ihsan Andi Dagong2*, Sri Rachma Aprilita Bugiwati2, Wempie Pakiding2 

...eat characteristics, and sarcomere properties of Kalosi chicken, which were grouped according to high and low Residual Feed Intake (RFI) phenotype. This study used 64Kalosi chickens kept intensively in individual cages. Chicks were reared from 24 to 70 days old. Data on feed consumption and body weight gain were collected every week. The residual feed intake value was calculated based on the average daily feed intake (ADFI), average daily gain (ADG) and mid-bo...

Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5 

...in 50 random bovine skin samples. Each sample was kept in a separate sterile plastic bag and transferred to the lab in an insulated icebox for detection of the presence of ivermectin residues using the high-performance liquid chromatography (HPLC) technique. Skin histopathology and immunohistochemistry of collagen were performed. Results: Ivermectin was detected in 36 samples, out of them ...

Mochamad Rizal Umami1, Zainuri*2, Sebastiana Viphindrartin2 and Rafael Purtomo Somaji2

...informants by multistage sampling technique. The data processing includes data reduction, data display using Decision Explorer version 3.3 software. Data were analyzed through descriptive methods and content analysis. The study found that the COVID-19 pandemic formed a new pattern of sugarcane trading system, switching from a contractual trade model to a free market model (redeemer). The model refutes the theory stating that the contractual model will be more ...
Amber Iqbal1, Mahroze Fatima2, Syed Z. H. Shah3,*, Muhammad Afzal1, Moazama Batool4, Muhammad Bilal5, Naheed Bano6, Ayesha Khizar2 and Sumbal Iqbal7
...feeding trial, fish were sacrificed, fillets were stored in refrigerator at -20°C and analyzed on 0, 15 and 30th day of storage. Upon refrigeration the vitamin E fed fish showed reduced level of Thiobarbituric acid reactive substances (TBARS) at all storage days. The enzyme activities were improved by elevating the levels of vitamin E in diet as well as by storage period (P < 0.05) for all treatments. Fatty acid profile indicated that n-3, n-6 fatty aci...

Yar Muhammad Jalbani1,2*, Muhammad Farooque Hassan2, Kiran Nazish2 and Yang Shuming1

...hicken meat rapidly, the samples were analyzed on Day 0, 3, 5, 7 and Day 10 using near infra-red spectroscopy approach combined with chemometrics tool of data driven soft independent modeling of class analogy. The meat samples from day 0 were selected as target class and checked against other day samples. All the samples were accepted for training data s...

He-Cai Zhang, Tian-Ge Hu, Chang-Ying Shi, Guang-Wen Chen* and De-Zeng Liu

...rcation line and indispensable treasure house of natural resources and ecological barrier in North China. In order to explore the genetic diversity, genetic structure and demographic history of D. japonica in Taihang Mountains, the mitochondrial Cytb and nuclear ITS-1 sequences of 120 individuals from 20 populations were analyzed. The results showed that 46 haplotypes were detected in total, including 10 shared haplotypes and 36 private ones. The overall haplo...

Uzma Zulfiqar1, Mahroze Fatima2, Syed Zakir Hussain Shah3*, Muhammad Afzal1, Shazia Shamas3, Sadia Roshan3, Moazama Batool4 and Muhammad Bilal5

...ofile showed decrease in saturated and monounsaturated fatty acids (MUFAs). While, polyunsaturated fatty acids (PUFAs) were increased in all vitamin E supplemented diets compared to control group. Furthermore, vitamin E level of 80.62 mg/kg was found optimum for maximum growth performance in L. rohita fingerlings, beyond which no increase in growth was observed.

...

Sayed H. Al Habty1, Dina N.Ali2* 

...ogurt. 150 dairy product samples from yogurt, ice cream and Damietta cheese (50 for each) were collected from Assiut city, Egypt. Antimicrobial susceptibility against antibiotics commonly utilized in human and animals was tried using the disc diffusion method; PCR was applied on (MDR) S. aureus isolates for discovering of blaz, mecA and VanA genes. 38% of yogurt samples had the highest prevalence of S.aureus followed by Dami...

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

...0° E). A total of 65 samples of milk including 5 pasteurized milk samples from various sources were analyzed through scientific polymerase chain reaction (PCR) method including other microbiological laboratory techniques (Total plate count, Gram staining, Hydrogen sulphide, Citrate, Urease and Indole Tests). A high level of total plate count (TPC) and E. coli was observed from both raw and pasteurized milk. The results a...

Most. Shakila Sarmin1,2, Md. Ashekur Rahman1,2, Most. Farida Parvin2,3, Mst. Shefaly Khatun4, Kazi Ahsan Habib5, Wasim Sabbir6, Jun Ohtomi7 and Md. Yeamin Hossain1*

...aspis cordyla. Fish were sampled (male = 720, female = 793) from commercial vessels operating in the Bay of Bengal (BoB), Bangladesh between January-December 2020. All lengths (TL, total; FL, fork; SL, standard length), were taken using a fish measuring board to 0.01 cm as well as a digital balance is used to weigh body weight (BW) to 0.01 g precision. The size structure (length-frequency distributions, LFDs) indicated that the males with TL 22.0-22.9 cm and f...

Sumaria Maqsood1*, Muhammad Ali 2, Amna Shoaib3, Shahbaz Ahmad2 and Noor-ul-Ain2

...expenses for rearing the same number of larvae on chickpea based diet (1749/Rs.) and almost equal to the tomato paste-based diet (669/Rs.). Therefore, a modified diet based on wheat germ and tomato paste is suitable for mass rearing of S. litura for six generations for laboratory and field experimentation.

...

Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*

... of treatment, mice were sacrificed, blood was collected for biochemical analysis, and sex organs were dissected out for histological preparations. Administration of testosterone has significantly increased the body weight and decreased the gonad weight in both sexes. It drastically affected the histology of testes and ovaries, causing a reduction in the sperm count and motility with a parallel reduction in the acid phosphatase activity. It also directly reduc...

Muhammad Saif-ur Rehman1, Faiz-ul Hassan1* and Muhammad Sajjad Khan2

...anni, Rojhan, Lohani, Hissar, Hariana and Tharparkar) were analysed using RAPD markers. A total of 80 RAPD primers were used for PCR amplification of DNA from the cattle breeds. The PCR products were analyzed by agarose gel electrophoresis. Scoring of bands were performed to generate dendrogram using unweighted pair group method of arithmetic means (UPGMA). Six hundred and four amplified bands were observed from successful amplifications of 73 primers (average...

Saba Rehman1, Faisal Salih Hayat1, Sadia Norin2, Abdul Aziz1, Siddiq Ur Rahman1* and Noor ul Haq1*

Asim Zubair1*, Zohaib Atta Mehdie2, Iffat Batool3, Ghulam Akbar Malik4 and Ayesha Aziz5

... and2011 and now in 2022 same as previously 9.1 million acres agricultural land is affected by floods and now the flood situation is worse than the past. Being an agrarian country more than 70% labour force of Pakistan depends on the agriculture. Due to climate changes people living or depending on agricultural sector for their livelihoods are facing much serious problem as their production in agricultural sector is much less than before. People of Pakistan fa...

Talat Farid Ahmed1*, Muhammad Azeem Afzal1, Hashim Nisar Hashmi2, Hafiz Muhammad Yousuf2, Shamim-ul-Sibtain Shah1 and Muhammad Atiqullah Khan1 

...ravel/boulder mixed with sand/ sand stone and thin layers of clay. GWL at Sanjwal Cantt sites was at 50 ft average depth having low to moderate yield of water and the formation had dominance of sand with alternate layers of clay against resistivity between 25-86 ohm-m. The study concluded that electrical resistivity survey (ERS) is a sound method for measuring GWL in ...

Zaheer Ahmed Lashari1, Muhammad Saleem Sarki1, Saleem Maseeh Bhatti1, Muhammad Sachal Khokhar1 and Zohaib ur Rehman Bughio2*

...e prepared composts were sampled and analyzed for selected parameters. Afterwards, a field experiment was executed to study the effect of prepared composts and their integration with inorganic fertilizer on growth and yield of onion (Allium cepa L.). The treatments were: T1=Control (No NPK and/or Compost), T2=Recommended NPK (120-60-60 kg ha-1), T3=Compost 1 (15 tons ha-1), T4=Compost 2 (15 tons ha-1), T5 Compost 3 (15 tons ha-1), T6=Compost 1 (10 tons ha-1) +...
Farah Abid1,2, Mohammad Saleem1,3*, Tahir Maqbool4, Tania Ahmed Shakoori5, Faheem Hadi4, Tahir Muhammad4, Saira Aftab4, Yasir Hassan7 and Shabana Akhtar4,6*
...10 mg/kg). Antioxidant assays (superoxide dismutase and catalase) and histopathological examination were performed to determine the values of various parameters of inflammation and arthritis. Real-Time PCR technique was applied to analyze the expression levels of different cytokines and interleukins involved in inflammation. Real-Time PCR results showed a down-regulation of TNF-α, COX-2, IL-6, IL-1β, NF-κβ1and up-regulation of IL-4, IL-10...

Shaolin Zeng, Shuai Yuan* and Kai Liu

...njury in rats and its causative role. The differential expressions of miRNA in the rat myocardial tissue after post-ischemic treatment with sevoflurane were screened by miRNA expression profile chip. The potential targets of miR-26a-5p were predicted by application of Targetscan, microT-CDS, miRtargetLink and miRpathDB database. The luciferin reporter groups were used for verification. We detected the impact of miR-26a-5p expression content on the PTEN/PI3K/Ak...

Saima Rubab1,3*, Ghazala H Rizwani2, Arjumand Iqbal Durrani3, Iram Liaqat4*, Urooj Zafar5, Mahjabeen6, Farah Batool7, Noor-E- Seher3, Naveera Younas3 and Ayesha Sadiqa8

...s have been used for thousands of years in different region of the globe for the treatment of various diseases. In developing countries, traditional medicines are used as source of primary health care. Keeping in view the importance of Camellia sinensis L., present investigation was aimed to evaluate the phytochemical and pharmacological potential of different morphological parts of C. sinensis L. Successive extractions of all plant parts was performed with di...
Serkan Özkaya1*, Oktay Özkan2, Sabri Erbas3, Isa Gökhan Yalçın4, Dariusz Piwczynski5 and Wojciech Neja5 
... by-product, can be used safely in alleviating the problems such as oxidative stress that may occur in intensive growing systems.

...

Lei Zeng1,2,3,4, Pimao Chen1,2,3,4, Zhenzhao Tang1,3,4, Jie Yu1,2,3,4 and Guobao Chen1,2,3,4*

...atus, Charybdis callianassa and Harpiosquilla harpax, showing a distinct preference tropism to AR, were turned out to be the main factors contributing to the spatial heterogenity of nekton community between A and B, which was closely related to dissolved oxygen (DO), water transparency (Tra) and total inorganic nitrogen (TIN). The findings in this paper are significant for the protection and restoration of crustaceans resourcs along coastal water, given the ra...

Ahmed Fathy1, Rania A. Hassan2, Essam S. Soliman3* 

...-validation, and holdout samples were 83.1, 82.6, and 82.2%, respectively. The three benchmarks used to judge the goodness of the model (Maximum chance = 83.1, proportional chance = 66.2, and press Q = 134.4) showed a model with good predictive power.

Keywords | Baladi buffaloes and crossbred, Canonical Correlation, Clinical Epidemiology, Discriminant Analysis, Productive and Reproductive Traits.  

...

Ghazala Nasreen1, Ali Hussain2, Komal Tayyab1, Muhammad Rashid3 and Sumaira Aslam1*

...re recorded in the liver samples of the fingerlings of the treatment group T1, while the lowest corresponding figures were noted for the fingerlings of the treatment group T4. These values were lower than found in the control group having 10.50±0.23, 77.25 ± 1.19, 24.50 ± 0.96 and 18.25± 1.19 IU L−1 levels for the ACP, ALP, AST and SOD, respectively. The hyperproduction of the hepatic enzymes revealed dysfunction of the livers...

Xuan Liang, Rongfang Zhang, Yan Xu, Hui Yang, Li Guo, Feiyang Na and Donghai Liu*

...groups A and B, faster disappearance of high fever, cough, and pulmonary rales than the control groups A and B, showing statistically significant difference (P<0.05); after 2, 4 weeks of treatment, the observation group had higher contents of trace elements Ca and Zn than the control groups A and B, lower TLR2 and TLR4 levels than the control groups A and B, showing statistically significant difference (P<0.05); after 2, 4 weeks of treatment, the observa...

Nourhan M.M. Hassnin, Ali H.H. Ali and Hosny H. Kesba*

... (6001 F1, BS F1, Ohbkatosa F1, and RG F1) as rootstocks, watermelon hybrids (Aswan F1 and Nems) as scions, and their accessions to Meloidogyne incognita was detected. Nematode penetration, development, and potential reproduction were estimated. The rootstocks (6001 F1, Ohbkatosa F1, and RG F1) were considered highly resistant and BS F1 resistant. Scions (Aswan F1, and Nems) were moderately susceptible. Grafted watermelon hy...
Alia Tajdar1,3, Shaghef Ejaz2, Muhammad Shah Zaib1, Anum Ishfaq1, Syed Muhammad Zaka1*, Hafiz Muhammad Safeer1 and Khalid Abbas1
Chunlan Shan1, Chaoying Liu1, Qin Lu1, Guowen Fu2, Syed Aftab Hussain Shah3, Rana Waseem Akhtar4, Ru Zhao2, Libo Gao2, Chang Liu2, Shushu Miao2, Hongdan Wang2 and Hong Gao2*

 

Amal G. Abdelrahman1, Nabil A. Yassien1, Hussein M.H. Mohamed1, Khaled S. Tolba2, Heba H.S. Abdel-Naeem1* 

... that all enzyme-treated samples significantly increase protein content and significantly decrease the fat content, pH, and thiobarbituric acid value with a significant improvement in raw and cooked sensory attributes as compared to their counterpart control samples. Such effect was more pronounced in spent hen meat patties treated with a mixture of kiwi (5%) and pineapple (5%).

Keywords | Spent hen patties, Tenderiz...

Doaa H. Assar1, Rasha A. Al Wakeel2, Mahmoud M. El-Maghraby3, Mohammed M. El-Badawy3, Adel A. El-Badawy3, Wael M. Nagy3, Mustafa S. Atta2, Abdel-Khalek E. Abdel Khalek4* 

...hree groups were fed the same diet supplemented with allicin at the level of 0, 0.4, 0.8, and 1.2 g/kg, respectively. The experimental period lasted 150 days (45 d prepartum, 60 d suckling, and 45 d post-partum. Results showed that allicin (0.8 and 1.2 g/kg) had positive impacts (P<0.05) on ewe weight prepartum, and at lambing, suckling, and mating. Milk yield as 6% fat corrected milk, and fat and total solids per cent in milk increased (P<0.05) by allic...

Sheeza Iqbal* and Asima Azam

...was conducted at Qazi Hussain Ahmed Medical Complex from March to July 2020 in district Nowshera, Pakistan. A total of 150 patients with sciatica were analysed. All the patients were diagnosed with MRI, CT scan, spinal X-rays and straight leg raising (SLR) test. Of the 150 sciatica patients, 88 (58.7%) were females and 62 (41.3%) males. The prevalence of sciatica was found to increase with age, heavy lifting, occupational workload and an inactive lifestyle. Sm...

Ilhama G. Kerimova1*, Viktor A.Krivokhatsky2, Merve N. Aydemir3 and Lala N. Mamedova4

...ontidae). Thus, the proposal to merge the Myrmeleontidae and Ascalaphidae into one family as suggested by Machado et al. (2018) is not supported by our data. 
 
Novelty Statement | The article presents the first report on the DNA analysis of the lacewing insects of Azerbaijan. A total of 25 species of antlions, mantidflies and owlflies were DNA barcoded.
...

Ghulam Akbar1*, Muhammad Anjum Zia1, Amer Jamil1 and Faiz Ahmad Joyia2

...on study. There are some salts like KH2PO4, K2HPO4, NaHCO3, CaCO3, CH3COONa.3H2O and FeSO4. 7H2O have been optimized for the production of SK. The K2HPO4 has shown the highest enzymatic activity with UV-Et mutant 680.0 UmL-1 and with CaCO3 UV-Et mutant has been found to give the relatively lowest enzyme activity 652.01UmL-1. While UV-mutant has shown maximum enzyme activity 275 UmL-1 with the addition of K2HPO4 and this mutant shown minimum 57 UmL-1 with the a...

Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2

... and alkaline pretreated samples of sugarcane bagasse using a fermentation process. Amylase was partially purified from the bacterial strain N1 and its activity was checked at distinct pH and temperature. The optimum pH was from 6-8 whereas the temperature was 40 °C. Saccharomyces cerevisiea was added for enhanced fermentation and ethanol production. The acid pretreated sample produced the highest ethanol (15.2%) than th...

Muhammad Yaseen1*, Muhammad Luqman1*, Bushra Pervaiz2, Rana Shahzad Noor3, Muhammad Ameen4, Sadia Hassan3 and Wassi Abbas1

...limate change, natural disasters and natural calamities. Therefore, rural advisory services to improve food security among the farming community are imperative. The present study was designed to explore various rural advisory service mechanisms for improving food security in the Sargodha district. The sample size of the study was 120 farmers. An interview schedule was used as a research instrument for data collection using t...

Faisal Khurshid1*, Asad Ullah1 and Sana Manan2

... using stratified random sampling technique. A sample size of 322 farmers was selected randomly from three villages namely Palosai, Badizai and Shahi Bala of the District Peshawar, Pakistan. The conceptual framework of the study comprised of an independent variable (knowledge of innovations) and dependent variable (adoption of agricultural innovations). Chi-square test was applied to ascer...

Shabana Parveen1*, Naeem Tariq Narejo2, Ayaz Hussain Qadri1, Ghulam Dastagir3, Muhammad Hanif Chandio4, Pervaiz Khan4, Faheem Saddar5, Naveed Ahmad Abro6, Shahnaz Rashid7 and Ghulam Abbas7

...an was examined from 240 samples (120 for each sex) during October 2020 to September 2021.The experimental fish was procured from the commercial catch of local fisher community. The investigations were based on estimation of fecundity and its relationship with other body parameters, variation in egg size and calculation of gonadosomatic index. The results of fecundity estimation were based on 10 mature female fish ranging in size 30.4 to 43 cm and from 235.0 t...

Umar M. Bello1*, Samuel A. Ojo1, Abdurrahman Ghaji1, Ambrose A. Voh (Jr)2, Muazu N. Bappah3, Casmir O. Igbokwe4  

...nt with two luteolytic dosages of PGF2α analogue (Lutalyse®, Dinoprost tromethamine), 12.5mg administered intramuscularly in the gluteal region, 11 days apart. Vaginal smears were stained using a modified Papanicolaou method while the vaginal or rectal temperatures (oC) was recorded with a standard (dry-bulb) clinical thermometer taken from 07:30 to 08:30 hrs daily and monitored throughout the period of experiment. In vaginal cytological smear ex...

Adrial Adrial1*, Rudy Priyanto2, Salundik Salundik2, Ahmad Yani2, Luki Abdullah3 

...at peatlands of Pulang Pisau district, Central Kalimantan Province through observation method supported by laboratory analysis. Reproductive performance data were obtained through direct interviews and recording cards. Soil and forage samples were taken from the main forage source locations. A total of 138 blood samples were taken through the jugular vein from Bali cows and their crossbred...

Ibrahim Samir Abd El-Hamid1, Alaa Emara Rabee2, Moustafa Mohamed M. A. Ghandour2, Rasha Salah Mohammed3, Ahmed Mohamed. Sallam4 

...eor), and garlic (Allium sativum). The third group (n=5) supplemented with 5 g/head/day of commercial Quebracho tannins extract (QT). The findings showed that the PHM and QT groups had higher (P≤0.05) mean values of sperm concentration, mass motility, sperm motility, sperm viability, and cell membrane integrity. Plasma testosterone concentration and number of red blood cells were higher (P≤0.05) in both treated groups. However alanine aminotransferase, c...

Samiya Rehman*, Eman Mustafa, Ali Ahmad Faiz, Maheen Kanwal, Farkhanda Yasmin and Arooj Fatima

...n. As the rate of drugs usage has increased from era of industrialization, so now it is prevalent everywhere in rural and urban areas also in educational institutions of different countries of the world. Drug addiction has impacts on health and caused unbearable mental as well as emotional pain. Brain functioning is adversely affected by drug addiction, which causes reduction in cortex of brain, alteration in structure and function of myelin sheath, neuroinfla...

Shahid Hameed Khan Khalil1*, Abdus Subhan3, Ghani Akbar1, Muhammad Asif1, Zafar Islam1, Adnan Shakeel1 and Fawad Anwar2 

...onents of wheat. The thousand grain weight was recorded higher in pots receiving 25% irrigation water. Grain yield was found to be higher with the conventional irrigation method, which was statistically at par with the treatment that used 75% of field capacity irrigation water. The harvest index was recorded higher in the treatment that was irrigated at 50% field capacity. Conclusively, 50% of field capacity irrigation with water was an effective treatment and...

Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1 

...) of stem cells have the same effects on target cells that their parental stem cells act, these effects are brought on by paracrine signaling involves the release of cytokines and nucleic acids (DNA, mRNA, and miRNA) which may lead to alteration of the gene expression, proliferation, and differentiation of the recipient cells. Methodology: 24 male New Zealand albino rabbits were used in this study, divided randomly into 4 groups (6 rabbits per group), inducti...
Wasim Akram1, Azhar Hussain2*, Sartaj Ali2, Iqbal Hussain3 and Muhammad2
...zonata and Bactrocera dorsalis, in apricot fruit orchards. Results revealed that there was a significant difference between the species and month to month. B. zonata had a substantially larger population than B. dorsalis. To predict the spatial variability of fruit fly species, geostatistical analytic techniques were used. Using an inverse distance weight with a spherical semivariogram model, the spatial distribution maps we...

Müge Aliye Hekimoglu and Sırma Sönmez

...quariums. Based on the basal diet (55% crude protein+14% oil), the four experimental diets were designed with supplementation of L-carnitine (0, 500, 1000, 2000 mg/kg). They were fed 10% of their weight every day. Sampling was done at the beginning and the final day of the experiment for digestive enzymes (protease, lipase, amylase), weight and length, body colour and survival rate. The image analysis method was used to determine skin colour. The chemical anal...

Quang Minh Dinh1*, Tran Thi Huyen Lam2,3, Y Thi Nha Nguyen4, Thanh Minh Nguyen1 and Tien Thi Kieu Nguyen5

...(26–36 °C) and salinity (3.8-37.25%), but data on this fish species’ food and feeding habit are still fragmented and deficient. Consequently, this research aims at supplying necessary data related to diet compositions and feeding activity of Butis koilomatodon collected in six estuarine areas of Mekong delta from April 2019 to March 2020. A total number of 1,227 specimens of Butis koilomatodon (891 males and ...
Xiao Zhang1, Guoqing Shen1, Yang Wang1, Pengdan Huang1,2, Yan Shui2, Zenghong Xu2 and Huaishun Shen1, 2*
...xt-align: justify;">Microsatellites, also known as simple sequence repeats (SSRs), are a type of DNA marker that are highly polymorphic in the genome. However, the microsatellite markers available for the red swamp crayfish Procambarus clarkii are limited. In this study, a sizeable number of microsatellites were identified using the data of the P. clarkii genome survey generated from Illum...

Nam Thanh Nguyen1,2,3, Linh Manh Ha4, Anh Thi Nguyen5, Nam Hoang Chu5, Hau Duc Tran5, Hung Phuc Nguyen5 and Thuy Thi Ta6*

...(standard length, pre-dorsal-fin length, body depth, body width) showed higher growth rates in males than in females, suggesting a morphometric sexual dimorphism. The growth pattern of males and females were similarly higher than the isometric value of three, showing positive allometric growth in favorable environmental conditions. The slope values were higher in the dry season than in the wet season, but this was only significant in females. The higher intra-...

I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman 

...l after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 hours until the spermatozoa motility reached at least 40%. The results s...

Salma Bessalah1, Samira Jebahi2, Asim Faraz3*, Amel Raoufi2, Cem Tırınk4, Walid Dridi2, Mahmoud Daouad2, Hassib Keskes5, Touhami Khorchani1, Khaled Farah2, Abdul Waheed3 and Mohamed Hammadi1

...d the amorphous phases disappeared. XRD patterns showed a new peak at 2θ= 65.8° and a diminution of the relative intensity for the peak of 2θ= 20.54° after 30 kGy. FTIR measurements revealed structural deformations from 5 kGy through chain scission of C–H chains as well as the deformation of the C=O carboxyl groups with increasing γ-radiation doses. The camel skin was thus proved to be a source of gelatin. Irradiation induced a ...

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

...cal mastitis in 345 milk samples (Cattle n=173, Buffalo n=172) collected from indigenous bovines of district Rawalpindi, Pakistan. The milk samples were screened for S. aureus and the confirmed isolates were subjected to disc diffusion test, PCR, and SDS-PAGE analysis for the confirmation of methicillin resistance. The farm-associated and individual animal-associated risk factors were analyzed to check the association with S...
Shakeel Ahmed Wagan1, Akeel Ahmed Memon1, Qudratullah Kalwar2*, Asmatullah kaka1, Mohammad Farooque Hassan2, Hidayatulah Soomro2, Safia Kadhro3, Abdullah Sethar4 and Muhammad Jehangeer5
...group B animals received same treatment but Controlled Internal Drug Releasing Device (CIDR) (containing 1.38mg progesterone) inserted into vagina on 0 day of the treatment and removed on 7th day of the treatment. Group C (Control group) received 02ml normal saline on 0, 7th and 9th day of the treatment. The positivity of estrus in peak and low breeding seasons was higher in Group B (88.88% and 60%) followed by Group A (73.3...

Mian Shamas Murtaza1,*, Aysha Sameen2, Saima Rafique3, Muhammad Shahbaz1,*, Nabeela Gulzar4, Mian Anjum Murtaza5, Umar Farooq1 and Iram Hafiz6

...istics of low fat cheese samples when added up to the level of 0.5% and 1.0%, respectively.

...

Mustafa Erkan Özgür1*, Selim Erdogan2, Songül Aydemir3 and Hatice Yumusakbas2

...results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus mykiss but the quantities of total polyunsaturated fatty acids (PUFA) were less. Palmitic and stearic acid for SFA, oleic and nervonic acids for MUFA and linolenic, arachidonic, eicosapentaenoi...

Hanaa H.A. Gomaa1*, Shimaa A.S. Hasan1, Nashwa Harb1, Amal H.A. Gomaa2 and Maha Anani3

...as conducted on 94 serum samples of pregnant women in Ismailia City. The presence of herpes simplex virus type 1 and 2 antibodies (IgG and IgM) and viremia were evaluated by commercial ELISA and real-time polymerase chain reaction (RT-PCR) techniques. Our findings revealed a very high frequency of HSV-1 and 2 infections among the studied pregnant women with percentages of 74.5% and 98.9%, ...

Carla Meneguin Barbosa1*, Fabio Gregori2, Cairo Monteiro1, Thais Helena Martins Gamon1, Luciano Matsumiya Thomazelli1, Tânia de Freitas Raso2 and Edison Luiz Durigon1

...rus 1 (PSHv-1) is the causative agent of Pacheco’s disease that infects psittacine birds, highly transmissible and presenting high mortality rate. Here we describe the investigation of 98 sudden deaths of birds, belonging to 8 Brazilian native psittacine species, divided in two outbreaks, one year apart between 2016 and 2017, in a breeding farm in São Paulo State, Brazil. PSHv-1 was detected in 6 species (61 birds) through Nested-PCR, that tested ...

Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1

...ation patterns and dispersal of birds, as resting and feeding points become scarcer resulting in agglomeration of different species. One pathogen of greatest importance that may be dispersed by birds are the coronaviruses (CoVs). Despite its relevance and complexity, studies on the detection and genetic characterization of these viruses are scarce, especially in South American wild birds. Thus, this paper aims to detect and discuss the diversity of these agent...

Wafaa M.A. El-Nagdi1, Ahmed E.A. Mahgoob2, Mahmoud M.A. Youssef1*, Entesar H. Taha2, Mona M.S. Zayed3 and Nora R.A. Saleh1

... before, after or at the same time with Ne inoculation. On the basis of the average overall percentages nematode reduction, bacterial isolates added before and after nematode inoculation were equal in decreasing M. incognita numbers, as they achieved higher average overall reduction (69.8%), when Pf isolates were added with the same time with Ne. Also, number of galls recorded the highest overall percentage reduction (60.3%)...

Oluwatoyin Adenike Fabiyi1*, Mariam Temitope Baker2 and Gabriel Ademola Olatunji2

... protection is primarily saddled with replacing the synthetics. A promising technique is the application of bio-pesticides. Organic fatty acid esters (FAE) are reassuring materials with nematicidal activities. Medicinal plants are rich source of acid esters, hence Alstonia boonei (Apocynaceae) leaves were extracted cold in ethyl acetate. This yielded crude extract that was subjected to column chromatography (silica gel 100-120 mesh grade), which afforded fract...

S. Samina and Y.I. Erum†

...s were conducted and 150 samples of root and soil were collected from different locations of Kurram Agency. The detail morphological and taxonomical studies revealed a total of 26 species of plant parasitic nematodes belong to 17 genera, 13 families, 15 subfamilies and 3 orders while free-living soil nematodes revealed a total of 21 genera, 17 families and 8 orders. Overall percentage of plant parasitic and free-living nematodes was 40% and 60%, respectively. ...

M. M. A. Youssef† and W. M. A. El-Nagdi

... number of plants in the same pot increased (1-4 plants/pot), a higher number of nematode juveniles in plant roots occurred. Thus, plant density, such as 4 plants if produced it promoted the number of the hatched juveniles, galls and egg-masses in roots as compared to the plant densities of 1, 2 and 3/pot. However, number of juveniles in soil decreased with increasing plant density.

...

S. F. A. Awad-Allah 1 and M. S. Khalil 2

...nita infecting banana (Musa acuminata) cv. “Grand Nain” as compared to the nematicide fenamiphos 40% EC under laboratory and field conditions. Experiments were carried-out during two successive seasons (2016 and 2017). Results of the laboratory tests showed that the toxicity of VCT and Nemaless® to M. incognita second-stage juveniles (J2) was increased as their concentrations and exposure times were increased. The LC50 of VCT ranged from 30.86 ...

H. M. Hassan† and S. A. Ibrahim

...des, Steinernema carpocapsae and Heterorhabditis bacteriophora were tested against cotton leaf worm Spodoptera littoralis. All tested insecticides caused less mortality to the tested entomopathogenic nematodes. Magic smart significantly surpassed other insecticides causing the mortality of 11.6 and 13.8 % to the entomopathogenic nematodes S. carpocapsae and H. bacteriophora, respectively as compared to Coragen that caused sl...

Siti Dharmawati1, Muhammad Halim Natsir2, Hartutik Hartutik2, Danung Nur Adli2, Osfar Sjofjan2*  

...ail flesh (Pomacea paludosa) treated with bromelin enzyme (FSFIBE) on growth performance, relatively organ weight, and digestibility of Alabio ducks (Anas plathyrhyncos borneo). A total of 210 one-day old ducks male Alabio with an average body weight of 45.26±2.59 g were placed in colony-shaped pen, and each pen contained seven ducks. The trial design used was completely randomized design with five treatments and six replications for each one, consistin...

S. Ahmed†, Q. Liu and H. Jian

...stify;">(J2) mortality assay in vitro. About 40.16 % isolates showed 40.0% J2 mortality during 12 hours. Significantly
increased upto 86.0% mortality was observed in 24 hour exposures. Among them twenty six Bacillus strains were
identified by morphological, physiological and biochemical carbon source utilization using API 50CHB strips that
belonged to Bacillus cereus group. Bacillus cereus strain...

A. Beyan1, A. Seid†2 and H. Shifa1

...oot-knot nematodes and Fusarium wilt are among the most economically important pathogens of tomato. A study was initiated with the objectives of determining the interaction effect of M. javanica (MJ) and F. oxysporum f. sp. lycopersici (FOL) disease complex and evaluating the response of selected tomato genotypes against this disease complex under glasshouse condition. A glasshouse experiment was laid out in a factorial randomized design with 18 treatment comb...

A. Ghasemzadeh1, S. Jamali1†, M. Esfahani2 and H. Pedramfar1

...tage juveniles, and four sampling times (20, 40, 60 and 80 days after inoculation). After sampling, purification, identification, and population of root-knot nematode species and race were determined, and after amplification of purified population on the tomato cultivar cv. Rutgers, inoculums were sufficiently obtained. In four-leaf stage of the plant growth, the nematode inoculum levels were introduced, and photosynthetic p...

S. Feroza1, A. G. Arijo1†, F. M. Bilqees2 and M. S. Phulan1

...nd inflammatory cells, disarrangement of hepatici and shrinkage of hepatocytes were found to have resulted into dilation of sinusoids.

...

R. Hadadfar1, E. Mahdikhani-Moghadam2†, S. Baghaee2 and M. S. Bajestani1

...istachio gardens of Khorasan Razavi Province of Iran, 50 soil and plant samples from pistachio roots rhizosphere (Depth 30-50 cm) were collected during years 2016-2017. Samples were transferred on ice to laboratory and nematodes were extracted by centrifugal methods. Psilenchus species were identified on morphological and morphometrical characters based on recent valid keys. Seven species from Psilenchus genus were identifie...

H. M. Hassan, M. M. Tantawy*, A. M. Younes and M. O. Sayed

...vernorate. Soil and root samples were collected during fruiting seasons of grapevine in 2012 and 2013. The nematodes encountered were identified as Meloidogyne spp., Helicotylenchus spp., Longidorus spp., Pratylenchus spp., Tylenchulus semipenetrans and Hoplolaimus spp. Community analysis of these plant parasitic nematodes showed variability in nematodes population densities, frequencies, biomass and importance values in different Districts during 2012 and 201...

S. Miheret1, A. Seid2† and N. Hailu3

... Ethiopia. Soil and root samples were collected from 30 tomato farms represents six different localities of the two districts. The results revealed that the highest incidence percentage (80%) of the Meloidogyne species from root samples were recorded from three farms of district Kewet and three farms of district Efratana Gidim. Meloidogyne incognita and M. javanica were found infecting 70% of the surveyed tomato farms in mix...

I. K. A. Ibrahim1, Z. A. Handoo 2†, A. M. Zid1 and M. R. Kantor2

...istance of 4 cotton, 2 sesame, and 6 flax cultivars to the root-knot nematodes Meloidogyne arenaria (Ma), M. incognita race 1 (Mil) and/or M. javanica (Mj) was determined in green house studies. The results showed that the cotton cvs Giza 77, Giza 85, Giza 88, Giza 89 and sesame cvs Shandwil 1 and Toshka 1 were resistant (R) to the tested nematode species. On the other hand, six flax cultivars were tested, among them Giza 1 ...

W. M. El-Nagdi,1†, Z. , E. Ghareeb2 and E. M. Zayed3

...r beet genotypes Beta Rozsa and Jamon were considered as the best with high yield and resistant reaction to M. incognita; in contrast Starmon genotype had highly susceptible reaction while Jary, Mnro andVorosch genotypes had moderately resistant. Fodder root yield was positively and significantly correlated with root weight; meanwhile, it was negatively and significantly correlated with damage index and gall index, respectively. These findings indicate that se...

sans-serif; font-size: 14px; background-color: rgb(255, 255, 255);">A.H. Choshali1, S. Rezaee2, S. Jamali3, H. Reza3, Zamanizadeh4 and F. Rejali5

...mber of galls, eggs, egg sacs and J2) in both tolerant and susceptible cultivars. Inoculation of susceptible roots with AMF significantly reduced the nematode pathogenicity factors. Also the results indicated that the activity of both enzymes increased in the plant with AMF compared with the cucumber roots inoculated with M. incognita alone. Cucumber mycorrhizal roots showed the highest mean activity of chitinase and ß 1, 3 glucanase respectively on the ...

M. Israr1†, M. Habib1 and K. Nasira2

...lign: justify;">The soil samples were collected from underground vegetable fields of Punjab and Khyber Pukhtunkhwa provinces. The plant parasitic nematodes were extracted from soil samples by Cobb’s sieving and decanting method (Cobb, 1918) followed by modified Baermann technique (Baermann, 1917). After processing, the nematode genera and species were identified (Siddiqi, 2000). Tylenchorhynchus usmanensis is reported ...

Urooj Shakoor* and Syed Attaullah Shah

...that 46.6 percent of the sampled rural households were multidimensional poor and the derived Multidimensional Poverty Index (MPI), at 0.30 cutoff level, was 0.254 for the overall rural Khyber Pakhtunkhwa. The decomposition of the household’s average deprivation level revealed that ‘living-standard’ and ‘health’ contributed more than 75 percent to overall poverty collectively. Regression analysis identified head’s age, educat...

Shafia Riaz1, Khalid Abbas1*, Tanveer Ahmed1,2*, Huma Naz3, Hina Amjad1 and Shahbaz Ahmad1

... (FQB), Lahore (LHR), Faisalabad (FSD), Bhalwal (BHL) and Qadirabad (QBD) assumed as five distinct genotypes were compared for genetic variability in relation to growth performance. Appraisal fish fingerlings were reared in glass aquaria (twenty fish per population) for 60 days under similar environmental conditions. Condition factor (K) and growth parameters (wet weight, fork and total length) were measured fortnightly. The...

M. Israr1, F. Shahina2† and K. Nasira2

...all stylet with minute basal swellings (stylet: 7-9 μm); vulva at 67-69 percent of body, tail short with pointed mucro (tail = 25-30 μm); and excretory pore situated just behind the median bulb, anterior to nerve ring. Female have a short post vulval uterine sac extending 25-34% of vulva-anus distance. Also included is the first record of A. siddiqii Fortuner, 1970 from around the roots of carrot (Daucus carota L.), fr...

E. Mahdikhani-Moghadam†, J. A. A. Bub, S. B. Chery and S. Alvani

...hida. A total of 36 soil samples were collected in the summer of 2014 from the rhizosphere of
stone fruits in different areas of the province. Soil analysis results in the identification of 5 species of mononchid
nematodes viz., Anatonchus kashmirensis, A. tridentatus, Clarkus papillatus, Mylonchulus brachyuris and M.
paitensis. Among them, A. kashmirensis is recorded for the first time in Iran.
...

A. M. Rahoo1, 2, †, T. Mukhtar3, B. A. Bughio4, S. R. Gowen1 and R. K. Rahoo5

...nship between nematode dosage and infective juveniles (IJ) production are
available for Steinernema affine. Therefore, in the present study the effect of inoculation doses of S. affine and
extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater
emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction
<...

M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1

...L.) and nigella (Nigella sativa L.) on root-knot nematode, Meloidogyne javanica @ 1500, 2500 and 5000
juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments
reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the
nematode inoculum increased, reproduction factor also increased while extract treatments effect decr...

S. A. Montasser1†, N. A. Mahmoud2, A. F. El-Mesalamy2 and M. A. A. Abdel-Mageed2

A. S. A. Saad1, M. A. Radwan2†, H. A. Mesbah1, H. S. Ibrahim3 and M. S. Khalil3

... nematicides namely, cadusafos, ethoprophos, fenamiphos, fosthiazate and
oxamyl for managing the root-knot nematode, Meloidogyne incognita in tomatoes based on number of galls per
root system, number of egg-masses, eggs per egg-mass and number of juveniles (J2) in the soil as well as plant
growth characteristics. All nematicidal treatments reduced the incidence of root-knot nematodes when compared

J. Salma, K. Nasira, M. Saima and F. Shahina†

...a diverse species, universally rich and
often showing sensitive responses to ecological changes. During present study, surveys were conducted at four
locations of Sindh coast viz., Kaemari, Korangi Creek, Ibrahim Haidry, Mubarak Village and two of Balochistan
coast viz., Gadani and Sonmiani beach. As a result, four new species Oncholaimus paraoxyuris n. sp.,
Metoncholaimus medispiculatum n. sp., Theristus (P) kar...

I. K.A. Ibrahim1, Z.A. Handoo2† and A. B. A. Basyony1

...enty-eight soil and root samples were collected for the detection of cyst nematodes. The results showed
the prevalence of nine cyst nematode species associated with different crop plants: Heterodera avenae on wheat, H.
daverti and H. trifolii on Egyptian clover, H. leuceilyma on Bermuda grass, H. lespedezae on lentil, H. goldeni on
qasabagrass, H. schachtii on cabbage and sugar beet, H. ze...

M. Behdani1†, F.J. Afshar2, M.R. Mirzaee1

...rgaz region), South Khorasan province of Iran infected with root-knot
nematode. On the basis of perineal pattern the nematode was identified as Meloidogyne javanica and is the first
report on B. vulgaris in Iran.
...

K. A. Tabassum,† J. Salma and H. Sagir

...t
of 20 soil samples, 6 samples were found positive for Steinernema species. One sample of Prunus avium had
Steinernema affine from Hunza, two samples had S. cholashanense from Juglans nigra and Malus pumila from
Gilgit, and three samples harboured S. feltiae from Prunus armeniaca, P...

C. C. Famina1†, A. Usman2 and M. K. M. Nasser1

...odes in soil
samples associated with banana (Musa paradisiaca), a survey was conducted in the Kondotty Taluk of Malappuram
district, Kerala, India. A total of 12 genera of nematodes including six commonly occurring nematodes in the banana
rhizosphere, three new records from banana and one new unidentified genus were obtained during the survey. Two
f...

Mutala’liah, S. Indarti † and N. S. Putra

...ted in
Malangsari, Getas and Candiroto feilds. Samples of soil and roots were collected and various parameters of soil
abiotic factors (pH, soil moisture, soil temperature, soil texture and organic matter) were obtained from each of the
three fields. Vertical distribution was assessed using two soil sample depths (30 cm and 50 cm). This research
showed that the highest populati...

M. A. Radwan†, M. M. Abu-Elamayem, S. A. A. Farrag and N. S. Ahmed

...inducing chemicals viz., salicylic
acid (SA) and its two derivatives; acetylsalicylic acid (ASA) and 3,5-dinitrosalicylic acid (DNSA), along with Lascorbic
<...
K. Nasira, S. Shamim and F. Shahina†
...Goth Detha Hyderabad; Khesana Mori near Tandojam; Mirpurkhas and Jamesabad (Kot Ghulam Mohammad).
Samples from different sources of freshwater viz., river, lake, canal, tube well, pond and irrigation water chennal
were examined for the prevalence of nematodes with an aim to disseminate the knowledge of fresh water nematodes
as indicators in environmental monito...
E. Yavuzaslanoglu1†, U. Gozel2, C. Gozel2 and M. Aydogdu3
...optera: Pyralidae). Soil samples were
collected from 130 apple orchards in April 2012 and 2013. Entomopathogenic nematodes were found in 25 samples
(19.23%). Entomopathogenic nematodes species isolated: Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida:
Heterorhabditidae) (16.92%) and Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae) (2.3%).
Entomopathoge...

H. Ravindra1, N. Adivappar2, M. Sehgal3 D. M. Soumya4 and H. B. Narasimhamurthy4

...the roots. Soil and root samples were collected for analysis of nematode infestation. Coriander
plants also exhibited poor growth showing stunting and chlorosis due to severe infestation. This is the first report of
root-knot nematode from Karnataka.
...

D. A. Darban1†, S. R. Gowen1, B. Pembroke1 and F. Hussain2

...treatment. The soil bioassay after
first harvest showed few juveniles encumbered with an average of 1-5 spores and the number of juveniles without
spores very high.
...
A. M. Khan1,†, S. Naz1 and M. Abid2
...weed Melanothamnus afaqhusainii using its powder and different extracts
including n-hexane, DMSO and water extract against naturally occurring soil nematode namely Meloidogyne
javanica anti fungal activity against Fusarium moniliforme and Rhizoctonia solani and fertilizing potential on okra
Abelmoschus esculentus. It was observed that the soil sample whi...

K. A. Tabassum, F. Shahina†, K. Nasira and Y. I. Erum

.... punctata n. sp. and O. sacchari n. sp., are described by both morphological and molecular
means from different agro-climatic regions of Sindh, Punjab and Azad Jammu & Kashmir, Pakistan. All species
belong to insectivora group on the basis of leptoderan bursa, crochet needle-shaped spicules, normal rectum, lateral
field with six separate lines and had unique ribosomal DNA-ITS, sequenc...

I. K. A. Ibrahim1 and Z. A. Handoo2

...tal of 240 soil and root samples were collected from
the rhizosphere of the surveyed plants. Twenty-three genera of phytoparasitic nematodes were detected in the
collected soil and root samples. In soil samples from Alexandria governorate, the sugar beet cyst nematode
(Heterodera schachtii) was very common on sugar beet while the root-knot nematodes Melo...

I. K. A. Ibrahim1, M. A. EL-Saedy2, S. F. A. Awd-Allah3 and Z. A. Handoo4,

...incognita on rice (Oryza sativa) cultivars 'Giza 178' and Sakha 101' were studied in the greenhouse.
Inoculation with H. goldeni alone or one week before inoculation with M. incognita on rice cv. Sakha 101
resulted in a significant increase in the number of cysts of H. goldeni as compared to plants inoculated with M.
incognita concurrently or a week beforehand. When H. daverti or H. zeae were inoculated one week after
...

Y. I. Erum and F. Shahina†

...been made regarding bioassay and
molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the
rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.
...

K. Nasira† and S. Shamim

...nd fungivores shared the same occurrence percentage (5%) while the least occurrence percentage was of omnivores
(2.5%). These studies add to the knowledge of diversity of fresh water nematodes and evidence on their
categorization of the basis of feeding habits and the community structure.
...

M. S. Yasir†, A. K. Sajid, N. Javed and M. Ghani

...vey, a total of 160 root samples were collected from different localities and
cultivars of date-palm. The maximum prevalence was recorded from locality Nouroz Kalat (100%) and the
minimum prevalence recorded was (20%) from Totazai and Raskoh. The incidence, as well as severity varied in all
the cultivars. The maximum severity and incidence of root-knot nematode was recorded from date-palm cultiva...

A. M. Korayem1†, M. M. M. Mohamed1 and S. M. El-Ashry2

...d of broad bean grown in sandy soil naturally infested with root-knot nematode Meloidogyne arenaria
was estimated during two successive seasons. Severity of nematode infestation (damage) was assayed at harvest
stage as root-gall indices (GI) based on scale of 1-6 (1= no infection and 6= 100% root galling). In the first season
(2014-2015) relationship between nematode damage and yield of br...

M. M. A. Youssef1†, Wafaa M. A. El-Nagdi1, and Mona G. Dawood2

...
dry leaves at the same rate caused the reduction 81.6%. Average nematode reduction (79.5%) was caused by using
fresh leaves of sugar beet at the highest rate followed by that occurred by the lowest one compared to untreated
control. Plant growth, number of nodules (produced by nitrogen fixing bacteria, Rhizobium) and yield followed the
same trend as the highest rate of sugar beet re...

A. A. Galal1,2, S. F. Abou-Elwafa1,3, F. J. Kopisch-Obuch1 and C. Jung1

...ght was
necessary to calculate the relative number of nematodes/g root mass. In conclusion, results led to a more efficient,
quicker and more accurate measurement of barley infections by Pratylenchus spp. which is important for selection
during early generations of cultivar development.
...

Ahmed S.M.H. EL Roby

... mealybug Saccharococcus sacchari under laboratory conditions (27 ± 2 °C,
RH 65 ± 10%). The EPNs used in the study are Steinernema sp. (strain: AT4), Steinernema sp. (strain: EMB),
Heterorhabditis bacteriophora (strain: EKB20), Heterorhabditis sp. (strain: EIK) and Steinernema glasri (strain:
New Jersey). The bioassays were carried out on Petri dishes containing parts of ...

I. Kepenekci1†, S. Toksöz2, T. Atay1 and I. Saruhan2

...nema feltiae, S. carpocapsae and Heterorhabditis
bacteriophora) isolated from Turkey [S. feltiae (Aydin isolate), S. carpocapsae (Black sea isolate), H. bacteriophora
(Aydin isolate) and H. bacteriophora (Çanakkale isolate)] and from Kyrgyzstan [S. feltiae(KG3) and H.
bacteriophora (KG81)] were tested for the control of black timber bark beetle, Xylosa

Fawzia, I. Moursy1, Amira, Sh.Soliman1, A. E.M. Khalil2,Samaa, M. Shawky2 and A. A. Taher2

...v>Tagetes erecta, Allium sativum and Fenamiphos (10%G) were evaluated to control M. incognita. All the
treatments decreased the nematode population of M. incognita in soil and on roots compared with check. Data
indicated percent increase of fresh weight of the whole plant which was greatly improved in all treatments.
...

K. Taimoor1† and F. Shahina2

...ania coagulans, Achillea santolina, Ferula oopoda, Nepeta cateria,
Teucrium stocksianum and Fagonia cretica were screened for their efficacy test against Meloidogyne incognita both
in laboratory and pot experiments at doses of 0.5g/lit water (0.5% w/v), 1.0g/lit water (1% w/v), 1.5g/lit water (1.5%
w/v), 2g/lit water (2% w/v), 2.5g/lit water (2.5% w/v) and 3.0g/lit water (3% w/v). Zatoria multiflora, Achillea

I.K.A. Ibrahim1 and Z. A. Handoo2

...e 3 (Mi3) on rice (Oryza sativa L.) was studied in the greenhouse. Seventeen rice cultivars were tested for resistance to Mi1 and Mi3. The results showed that rice cvs Araby, Giza 159, Giza 170, Giza 171, Giza 172, Giza 177, Giza 178, Nahda, Sakha 101 and Sakha 102 were either susceptible or highly susceptible to Mi1. Rice cvs A95, IR1, IR22 and Japonica 47 were moderately susceptible to Mi1, whereas cvs IR28, IR459 and Philippini 24 were moderately resistant ...

S. Fatemy†1 and H. Ghasemi2

...e nematodes to root diffusates of these cvs was also investigated. Disinfected cysts of G. rostochiensis were placed in root diffusates and the numbers of hatched second stage juveniles (J2) were counted weekly over a 6-week period. Pots were planted with a single sprout of each cv. and the soil was inoculated with a suspension of 400 freshly hatched J2. There was a significant difference in the numbers of J2 that had entere...

Shaista Naz1*, Muhammad Arif2 and Akhtar Ali3

... developing world in the said subject not only shed light on the lessons learnt but also can identify the gaps left out. For this, a systematic review has been conducted to highlight the immense role of women in livestock and its determinants in the developing countries. To achieve these objectives, a total of 32 publications after following the inclusion and were reviewed. Content analysis has been used for analysing data and the study found that women have h...

Uzma Ishaque1, Nasira Kazi1, Erum Iqbal1* and Shahnaz Dawar2

...recovered. Mylonchulus musae n. sp., collected from the rhizophoric soil of Musa paradisiaca L. in Karachi Sindh, Discolaimus tabacum n. sp., from the roots of Nicotiana tabacum L. in Manshera, KP, Discolaimus conicus Siddiqi, 2005 from the roots of Saccharum officinarum L. in Mirpurkhas, Sindh, Discolaimus laksi Khan and Laha, 1982 from soil around the roots of Gossypium hirsutum L. in Sargodha, Punjab, Discolaimus omanensi...

Farwa Batool1*, Riaz ur Rehman1, Samia Ikram2 and Maham Zahara3

...nt combinations of silt, sand, leaf compost, FYM, peat moss, sawdust and garden soil were used as (SGS) for the growth and quality of three selected foliage plants (A. commutatum, D. deremensis and A. elatiors). The plant response was observed through observing the plant height (cm), leaf length (cm), leaf area (cm), number of leaves (cm), stem diameter (mm) and survival (%). The pH, EC, potassium (K), nitrogen (N), phosphor...

A.A. Tanimola† and B. Fawole1

...ls:
tannins, saponins, flavonoids, cardenolides, phenols, alkaloids and anthraquinones. However, total phenol (14.3
mg/g), tannins (14.5 mg/g) and saponins (59.8 mg/g) were highest in AKY than in other aloes. Flavonoid content
(3.7 mg/g) was highest in AAR while alkaloid content was highest in AST. The infrared analyses revealed that the
Aloe species had similar functional grou...

A. Khan†, S.S. Shaukat1, K.A. Khanzada and M.S. Khan2

...s were applied including sawdust, sugarcane bagasse, neem (Azadirachta indica) leaf powder and
marigold (Tagetes erecta) flower powder used alone and in combination with Fertinemakil. Untreated pots were
kept as control. Carbofuran a chemical nematicide was used for comparison. Subsequently after 8 weeks nematode
populations were studied. The results showed that amendments significantly influence...

S. Ahmed†, A. Munir, S. Hameed, S. Asad, M. Fayyaz, M. Zakaria and M. Umer

...e survey a total 69 soil samples were collected from various agro-ecological areas with different
cropping history in six districts comprising Mianwali, Bhakkar, Gujranwala, Sialkot, Gujrat and Mandi
Bahauddin of Punjab province, Pakistan and assessed for nematode populations. Analysis of soil samples
revealed the presence of eight genera of plant parasitic and soil nematodes. The most fre...

S.A. Khan†, M. Abid and F. Hussain1

... and Melanothamnus afaqhusainii showed maximum egg hatching (96%) and larval mortality (99%)
and (100%), respectively in water and methanol extract @ 10% concentration after 72 hours exposure time.
Similarities between Ward’s cluster analysis of hatching egg and larval mortality in water and methanol extract of
different seaweeds showed significant difference. Methanol extract was more effective as compared to water ex...

D.A. Darban†, S.R. Gowen, B. Pembroke, F. Hussain1 and R.A. Memon2

...iment suggest that dispersal of spores from the degrading females occurs after 2 weeks
and this is reflected in a significant reduction in egg-masses, galling and the female population between the 1 week
and 3 weeks treatments. The greatest percentage of infected females and numbers of endospores were recorded in
that treatment where P. penetrans infected females were left to degrade for 3 weeks.
...

W.M.A. El-Nagdi and M.M.A. Youssef†

...ean, cowpea, maize and sesame plants
replacing sugar beet for controlling root-knot nematode, Meloidogyne incognita resulted that sugar beet-Hybrid
maize and sugar beet-sesame cropping sequences proved most effective against root-knot nematode as they reduced
nematode parameters as indicated by the number of galls, egg-masses and hatched juveniles on roots. Con...

A. Zia†, K. Zia*, S.A. Anwar** and M. Iqbal

...in different parts of Faisalabad district was conducted to identify the
association of root-knot nematode (RKN) species with tomato crop and to assess their infestation. Hundred and
sixty-one samples from 23 locations scattered over the vegetable growing areas of four tehsils of Faisalabad district
including Faisalabad...

M.A.M. El-Saedy, A.A. Mokbel*† and S.E. Hammad**

... onion
and sesame resulted in 48.0-92.7% reduction in numbers of root galls/root under laboratory conditions. Similarly,
application of these oils resulted in great reductions of 59.2-92.8% and 47.4-89.6% in numbers of root galls and
egg-masses/root when oils applied before and after transplanting of tomato seedlings, respectively. The highest
reduction (%) of galls, egg-masses and re...

A.W. Amin† and A.W. Mona*

...tocks, wax melon, Benincasa hispida (grafted
onto Bh) and C. maxima x C. moshata Ercola hybrid 6001 (grafted onto Ercola 6001), marigold (Tagetes spp.) as
root antagonistic plant and Rugby as a nematicide (two formulation, Rugby 10G and Rugby 20L) for control of
Meloidogyne incognita cucumber root-knot were evaluated in nematode naturally infested soil under greenhouse
conditions in t...

I. Umar† and A. Mamman*

...shes were treated to the same concentrations of the extract in Petri
dishes containing 1000 second stage juveniles of M. javanica. Egg hatch inhibition and larval mortality was
observed over a period of 72 hrs. Sterilized loamy soil (4 kg) contained in 36 perforated plastic pots (30 cm dia.
and 40 cm depth) were separately mixed with 0, 30, 40, 50 and 60 g (equivalent to 0, 10 15, 20 and 25 tons/ha) of
the fruit ...

M.A. Haq†, K. Mahmood*, M. Shahid*, S.A. Khan and A. Tahir**

...v>...

M. Shahi-Bajestani† and E. Mahdikhani-Moghadam

...v>In this study, 42 soil samples from onion fields of Razavi and Northern Khorasan provinces resulted 16 species of
nematodes viz., Psilenchus curcumerus, Ditylenchus apus, D. medians, Basiria gracilis, B. graminophila,
Aphelenchus isomerus, A. avenae, Boleodorus thylactus, Zygotylenchus guevarai, Pratylenchus thornei, P.
neglectus, Irantylenchus clavidorus, Neopsilenchus magnidens, Neopsi...

M.M.A. Youssef and A.M.S. Lashein†

...e the efficacy of acetyl salicylic
(ASA) and γ-amino-n-butyric acids (GABA) as chemical resistance inducer at the concentrations of 5, 10 and 20
mM against nematodes and consequently on date palm yield. The results showed that number of R. reniformis in soil
and roots and Helicotylenchus sp., were reduced in soil one month after application at the ...

A.A. Mokbel

...de in all collected soil samples followed by Tylenchorhynchus
spp., Helicotylenchus spp., M. javanica and Pratylenchus penetrans. All tested peanut cultivars were resistant to M.
incognita race 2 and moderately resistant to M. javanica. Whereas, peanut cvs. Balady, Ismailia 1 and Giza 6 were
found highly susceptible to M. arenaria race 1 and Giza 4 and Giza 5 cvs. were susceptible. The suppressiv...

F. Toumi†*,**, L. Waeyenberge*, R. Yousef***, H. Khalil****, K. Al-Assas*** and M. Moens*,**

...ority of the
sampled tomato cultivars were infected with two Meloidogyne species; once both species were detected on the same
variety.
...

A.A. Anter, A.W. Amin†, A.H. Ashoub* and A.S. El-Nuby*

.../font>R) viz., acetylsalicylic acid (ASA), DL-3-aminobutyric acid (BABA), 2,6-dichloroisonicotinic acid
(INA), 5-chlorosalicylic acid (CSA), nitrosalicyl ic acid (NSA

A.W. Amin†, A.A. Anter, A.H. Ashoub* and A.S. El-Nuby*

... and Pseudomonas aeruginosa recovered
from tomato rhizosphere and tested for their ability to induce systemic resistance or bio-control agent against
Meloidogyne incognita in tomato under greenhouse condition. Results showed that all tested bacterial strains
showed significant reduction in nematode development and reproduction. The most effective strains were M.
methanica, B. cereus, B. brevis and O. proteus. The...

W.M.A. El-Nagdi†, M.M.A. Youssef and M.G. Dawood*

...xtracts of garlic Allium sativum mashed clove and oil. The plant materials were
diluted with distilled water at concentrations of 2.5, 1.25 and 0.625% and drenched in each plot. Results showed that
the plant extracts showed nematicidal and nematode-hatching inhibitory activity reduced nematode criteria including
number of galls, egg-masses and hatched juveniles on roots of eggplant and number of juveniles in soil at harvest<...

A. Khan†, S.S. Shaukat*, N. Khatoon**, J. Akhtar and A.G. Rizwana**

...dex.
...

Aiman Amur1*, Nasreen Memon1, Khalida Unar2 and Roshan Jamali3

...world. The Bactrocera dorsalis (handle) complex fruit fly according to morphology, physiology and genetically. It is very serious pest of many fruits in the overall world; such as guava, apricots, Sapodillas etc. this species is voracious for the mango in whole world mostly; means the favorable host of delicious mango. During current study observe the ecological margins and its habitats, host effects on the occurrence of this decisive pest. This study was stee...

Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1* 

...t, Nigeria. 200g of each sample was washed in 200ml of 0.85% normal saline solution and taken through standard sedimentation procedures. The sediment recovered after centrifugation was applied on a grease-free microscope slide; a drop or two of Lugol’s iodine was added to it. This was then covered with a cover slip and viewed under the light microscope at x4 and x10 objective lens. Results: Only S. stercoralis was isol...

Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1 

...n, post-vaccination sera samples were collected and subjected to an HI test to determine antibody titers. The Haemagglutination inhibition test was done using Newcastle Disease Virus LaSota strain NLT 106.0 EID50 antigen. Post-hatch feed and water deprivation durations significantly affect the production parameters. Feed intake, body weight gain and average daily gain were significantly (p<0.01) lower in the T5 group. Significantly (p<0.01), a poor feed ...

Yuejing Yang1,2, Mengbin Xiang1,2, Zhengshi Zhang3, Minrui Zhou1, Bingjie Hu1, Tingsen Jing1, Zhe Li1, Fenglin Liu1, Hui Luo1, Qinglu Li1* and Hua Ye1*

...i was analyzed using 164 samples with the same growth conditions. Principal component analysis showed that the body weight accounted for 94.50% of the variance, the eigenvalue was greater than 1, and the accumulative variance was more than 85%. So, the body weight was the first principal component of the growth traits for S. prenanti. Association analysis indicated that ug25066-1502 had significantly associated with total le...
Ghulam Jelani1, Qudratullah Kalwar1*, Asmatullah Kaka2, Abdullah Channo2, Ayyaz Ahmed1, Majid Hussain Soomro1, Hidayatullah Soomro1, Deepesh Kumar Bhuptani1 and Yar Muhammad Jalbani1
...the factor that are necessary for transfer of embryo from donor to recipient for production of offspring. Previously limited information existing regarding embryo transfer and its significance in farm animals therefore, in future this review might be helpful in improving the reproductive potential of farm animals.

...

Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk 

...ds of testing biological samples from laboratory mice and rats were realtime polymerase chain reaction and enzyme-linked immunosorbent assay. A high prevalence of some pathogens among conventional laboratory animals used by some academic institutes and universities of Almaty has been shown. Rodentibacter pneumotropicus has been detected in the SPF animals, while no other pathogens from the FELA

Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4

...ion and disc diffusion assay against various disease-causing microorganisms. In the case of well diffusion assay, the plant exhibited a maximum 16mm inhibition zone against B. subtilis a minimum 10 mm inhibition zone against P. aeruginosa, with acetone extract. The same extract seems inactive against K. pneumoniae as compared to control. When tested on S...

Najam-ul-Sehar Afshan1*, Javeria Majeed1, Abdul Rehman Niazi1, Saima Khanum2, Maria Riaz1, Muhammad Fiaz2 and Shaila Anjum

... 2015–2020. The causal fungus was observed and identified on the basis of morpho-anatomical and molecular analyses. This is the first report of powdery mildew infection caused by Erysiphe necator var. necator from Punjab and Khyber Pakhtunkhwa provinces of Pakistan.

...
Jawad Ahmad1, Huang Yun-He2, Arab Hussain1, Amjadullah1, Ahmad Yar1, Subhanullah1, Zeshan Ali1, Hamid Sattar3, Tariq Hussain3, Hamayun Khan3, Ali Gohar6, Obaidullah4, Nan Zhu Fang5, Yanya Fang5, Ihsan Ali3* and Zhongshu Li5,*
... 4= non-pregnant). Blood samples were collected from all the animals and assayed for hematological biomarkers like erythrogram, leukogram and erythrocyte sedimentation rate (ESR) were determined during three stages of pregnancy. The highest RBC count was recorded in group 4, The highest Hb, MCH and MCV was recorded in group 3. The lymphocytes and neutrophils were high in group 2 and group 3 respectively as compare to other g...

Yanjie Liu

...Each group was given the same exercise intensity. Venous blood was collected before and after each test to determine changes in Immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), IL-2, WBC, natural killer (NK) cell activity, C3, C4, CH50 and body weight. We found that the levels of IgA, IgG, and IgM in the three groups decreased; while IL-2 levels increased, and the order can be ranked as control group>hypoxic group>high altitude gro...

Salman Hussain1, Basit Zeshan2*, Rabiya Arshad1, Saba Kabir1 and Naveed Ahmed3

...A total of 208 different samples were collected and processed for phenotypic and genotypic identification of MRSA. All MRSA isolates were subjected to antibiotics sensitivity, cefoxitin disk diffusion test, and vancomycin minimum inhibitory concentration (MIC) E-test. The MR

Kalsoom1, Nasir Shah2, Muhammad Ibrahim3*, Tahira Bibi1, Kazim Ali4 and Zahir Shah2

...ughout the world but its salt sensitive. A large portion of irrigated land in major countries is affected by salinity or is likely to be salinized in near future. This study assessed the impact of various salt levels on morphology and biochemical attributes of a wild (Sante wild) and two transgenic (Sante 2 event and Sante 8 event) potato varieties in in...

Hina Safdar1,2*, Nazir Javed2, Sajid Aleem Khan2, Muhammad Zeeshan Majeed3, Arif Mehmood3 and Muhammad Arshad4

...ar larvae. In other bioassay, 40% suspensions of bacterial metabolites were evaluated against different larval instars of S. litura and were found significantly effective against all larval instars. Maximum average mortality (52.80%) was observed in Xenorhabdus spp. derived metabolites against 2nd instar larvae as compared to Photorhabdus spp. In both bioassays, mortality was increased along with the exposure time and reache...

Ayesha Bakhtiar1, Sardar Azhar Mehmood2, Abdul Rauf Bhatti3*, Shabir Ahmad2, Naqash Khalid2, Javed Iqbal5, Azra Nadeem4 and Waqas Ahmad1

... E received active immunisation against ND using Lasota-1, Lasota-2, Mukteswar-1, and Mukteswar-2, respectively, leaving A as the control group left uninfected. On days 21 and 35 of the study, the serum HI antibody response to these four immunisations was assessed. As evidence of its strong efficacy, Group C, which had received the Lasota (vaccine-2) vaccination, displayed high antibody titers throughout the experiment. In t...

Rahim Bux Vistro1*, Mashooque Ali Talpur1, Irfan Ahmed Sheikh1, Muneer Ahmed Mangrio2, Rajish Kumar Soothar1, Mian Noor Hussain Asghar Ali3,4, Imran Ali Dhamach4, Muhammd Urs Mirjat1 and Abdul Saboor Soomro2

Sofiane Tamendjari1,2*, Farida Bouzebda Afri1,2, Lina Chaib1, Hebib Aggad3, Zoubir Bouzebda1,2 

...ine
A total of 90 samples of sheep, chicken and turkey meat were collected and analyzed. Modified Baird-Parker medium was used for enumeration and isolation of strains, free coagulase and thermonuclease tests were performed as well as other biochemical tests. Antibiotic susceptibility testing was performed to evaluate the resistance of S. aureus to antibiotics. Finally, statistical processing was performed using the STATISTICA 7 software (Statsoft, Fran...

Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4 

...E) supplemented with docosahexaenoic acid (DHA). Electro ejaculator was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were sequentially incubated for 15 minutes a...

Zubair Aslam1, Ali Ahmad1*, Korkmaz Bellitürk2, Hira Kanwal1, Muhammad Asif1 and Ehsan Ullah1

...rsity of Agriculture, Faisalabad in a tunnel under field conditions. The experiment had five treatments in which the mineral (NP) fertilizer, simple compost, earthworm’s excreta-based vermicompost and vermi-tea were alone and combined in different proportions as (T0: control (recommended fertilizers), T1: 100% simple compost, T2: 100% vermicompost, T3: 100% vermi-tea, T4: 33% simple compost + 33% vermicompost + 34% vermi-tea and T5: 25% simple compost + ...

Majid Ali*, Hafiz Abdul Majid, Farman Ullah, Tahir waseem, Muhammad Rashid Khan 

...m or herds. Random blood samples were received and collected from the surrounding village of arid zone research institute kohat. A total of 726 blood sample were analyzed from both sheep and goat. Out of the total blood sample (62.39%) total prevalence were recorded for all type of hemoprotozoa. In which highest prevalence was recorded for theleriosis 46.13%, followed by anaplasmosis 29.13...

Laveena Shekhawat, Sheethal S* 

...ence of the cysts in the same sites.

Keywords | Cystic ecchinococcosis, Ecchinococcus granulosus, Hepatic cyst, Parasitic cyst of liver, Liver parasite 

...

Muhammad Furqan1*, Zulfiqar Ali1, Muhammad Mudassar Shahzad2, Rida Ahmad1, Haqnawaz Yousaf3 and Imad Ul Din Zangi4

...ign: justify;">Kalij pheasant is habitat indicator species of ecosystem. The breeding biology of Kalij pheasant was studied in Northeastern Himalayan region, tehsil Nakyal, district Kotli, Azad Jammu and Kashmir, Pakistan from April 2020 to March 2021. Breeding activities of kalij pheasant were recorded from March to July. Breeding calls were recorded in March, April and nesting started in...
Aulia Evi Susanti1*, Rudy Priyanto2, Muladno2, Dewi Apri Astuti3, Lucia Cyrilla2
...mmunity (FS-SSC) Maju Bersama, Musi Banyuasin Regency in 2021. The feed application experimental design used a factorial 3x2 Randomized Block Design (RBD) with 10 replications. A descriptive analysis was conducted to evaluate the corporate model’s production system and business management. Production analysis was analyzed using ANOVA and data were processed using R studio software version 4.1.2. Furthermore, the observed parameters include average daily ...

Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie

...infected hundreds of thousands of livestock in Indonesia after being declared FMD-free for 32 years since 1990. As of November 18, 2022, FMD is still spreading in 17 provinces in 146 regencies/cities with the the number of infected cows 578.060 heads, recovered 508.494 heads, conditionally cut 13.177 heads, died 10.269 heads, and vaccinated 5.847.113 heads. This article is carried out using literature study and secondary data exploration methods. There are thr...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...ange of serodiagnostic assays for mycobacterium has been an area of interest through the past two decades as a rapid, accurate, and easy-to-be-used and interpreted diagnostic kit as well as to be a competitive alternative to the conventionally established expert-dependent techniques. The current work’s main objective is to evaluate the reliability of two widely distributed rapid kits for as a suitable substitution for the gold standard bovine tuberculosi...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...up of birds were fed a basal diet of soybean-corn meals and served as a control (0% BSFL meal), while the remaining 4 groups were fed a basal diet in which the soybean-corn meals were partially replaced with 3%, 6%, 9%, and 12% BSFL meals, respectively. The experimental trial continued for 10 consecutive weeks from 40 to 50 wk of age. One-way analysis of variance (ANOVA) including a polynomial test was carried out to explore...

Owolabi Olutunmise Victoria1*, Saka Olusola Stephen2, Olashinde Oluwaseun Ruth1, Alli Smith Yemisi Rufina3

...rchers are in search for safe drugs that can bring a total recovery from diabetes. Thirty (35) male wistar rats were divided into six (7) groups of five animals each to study the antidiabetic and antioxidative properties of young Cola acuminata leaves aqueous extract. Diabetes was induced using alloxan monohydrate.5 ml of normal saline was given to group I , group 2 received 5 mg/kg/ body weight of the standard drug glibencl...

Seema Perveen Memon*, Nasreen Memon, Mansoor Ali Shah, Reshma Sahito and Aiman Amur

Bashir Ahmad1*, Ali Muhammad Yousafzai2, Waqar Ali1, Ikram Ilahi1, Farman Ullah3, Saeed Ahmad1, Ayaz Ali Khan1, Umair Ahmad2 and Hafsa Maria2

...atase (ALP), alanine transaminase (ALT), and aspartate transaminase (AST). The outcomes of the extract-treated rabbits were comparable to those of the group of rabbits given silymarine. RBC, platelets, haemoglobin, MCH concentration, and HCT levels did not significantly alter after treatment with garlic aqueous extract and silymarine. However, a substantial (P 0.05) rise in the overall WBC count was seen. The current researc...
Magda A. Eldomiaty1,2, Manal E. Elsawaf2, Soad S. Ali3, Heba El-Sayed Mostafa4,5*
...level was conducted, and samples of adrenal gland were examined for structural changes by light and Electron microscope, and for immunohistochemical expression. Rats from group III showed statistically increased corticosterone level and increased cortical thickness compared to other groups. Voluntary exercise improved these measures in rats of group IV. Histological disorganization of cortical cells was observed in group III with manifest large cytoplasmic vac...
Shaker Al-Suwaiegh
...y were determined. Blood samples were collected for determination of plasma proteins, glucose, liver enzymes and minerals parameters. The results indicated that Azolla pinnata contain protein (32.83%), crude fiber (13.6%), ether extract (1.17%) and ash (3.89%). Upon feeding, replacement diet with 20.0% Azolla pinnata caused significant increase in feed efficiency and body weight gain. In addition, the plasma metabolites (total protein, globulin, and urea) and ...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
...ation with Infectious Bursal Disease Virus (IBDV) “IBDV/Egypt/Qalubia/17” and/or some commercial immunostimulants (Lector® and Orego®) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance and clinico-pathological changes. The results indicated that, immunostimulants can keep maternal immunity longer where lector® was the best; the decline in HI titers also indicates ...

Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2, Muhammad Shahroaz Khan1 and Muhammad Zeeshan Majeed1*

...ment. In laboratory bioassay, combinations of flubendiamide and A. indica, flubendiamide and N. tabacum and of spinetoram and A. indica formulations caused significantly high mortality (100%), followed by B. thuringensis and S. litura-NPV (94.92%) and A. indica and B. thuringensis (93.22%) and exhibited a synergized toxicity against S. litura larvae as compared to other treatments. In two years field trials, binary combination of flubendiamide and spinetoram s...

Aiman Amur*, Nasreen Memon, Reshma Sahito and Seema Memon

...e range of Bactrocera dorsalis in three different mango cultivars in sindh province of Pakistan during 2013-2014. Experiment was conducted on major three varieties of mango Chunsa, Sindhri, Beganpali, Data were noted on calculating in good physical shape and pest-ridden mango fruit; casually collected from major mango growing areas of sindh. Rearing the fruit flies under controlled conditions in Laboratory. Collected the rea...

Aamir Mushtaq1*, Fatima Habib2, Umar Farooq Gohar3, Abdul Malik4 and Mobasher Ahmad2

...administered with normal saline, P. anisum methanolic extract 400 mg/Kg, and 800 mg/Kg respectively for three months. The results of acute toxicity study represented the LD50, value as 4.1 g/Kg/po in albino mice and it was observed that the acute toxic doses produced behavioral changes like hyper-salivation, hyper-secretions, irritation, cyanosis and sedation in mice. Results of chronic toxicity indicated that plant extract ...

Tilahun Bekele* and Abebaw H. Georgis

... of black cumin (Nigella sativa L.) in response to seed rate. The experiment composed of four varieties (Dirshaye, Darbera, Aden, and local), and four levels of seed rate (10kg, 15kg, 20kg, and 25kg per ha) set out in a randomized complete block design and replicated three times. The findings of the statistical analysis exhibited that the majority of the response variables under consideration were significantly (P<0.01) influenced by seed rate, variety, and...

Nachaat Sakr

..."text-align: justify;">Fusarium head blight (FHB) can have a destructive effect of barley and wheat along the whole world. To defeat it, sources of host resistance and pathogenic variation of FHB species need to be identified. Although barley and wheat are crucial crops in the dried Mediterranean area, there is insufficient information about their resistance to head blight infection and aggressiveness of Fusarium species. A ...

Idrees Ahmad1, Muhammad Jamil2*, Hafiz Muhammad Zia Ullah1, Saira Saleem1, Syed Ahtisham Masood3, Umair Faheem4, Imran Akhtar5, Muhammad Abdullah6, Abdul Majid Khan5 and Naeem Iqbal7

... sowing to maturity. Thousand seed weight ranges from 5.10 to 5.23 grams. Its average stature is 157-171 cm and possesses tolerance against aphids and diseases especially Alternaria blight and White rust. It yielded the highest (2891 kg ha-1) at the NPK@ 80:40:40 level. The optimum yield was produced (1976 kg ha-1) when sown on 30th September. Its cultivation on a commercial scale will enhance the share of local edible oil production and will strengthen the na...

Zarema Alimsultanova Magomedova1, Kheda Khalitovna Dadaeva1, Roza Said-Akhmedovna Zakhkieva1, Islam Khasanovich Shakhbiev1*, Boris Kazievich Laipanov2 

...of soil, grass and water samples in the lowland, foothill and mountainous zones of the North Caucasus region (Chechen Republic) revealed a different degree of their infection with eggs of nematodes of the genus Nematodirus Ransom, 1907. In the lowland zone of the Chechen Republic, 37.50% of soil samples, 23.00% of water samples were contaminated with invasive elements, respectively, in the...

A. Yu. Aliev1*, A.A., Aliev1, A.M. Musaev1, M.Z. Magomedov2 

...o dense) of in the milk samples, which was partially (+) or entirely (++) of thrown of out the from the hole in plate with stirring. On the plate, with a negative reaction (-), the mixture of milk and the indicator remained homogeneous, pale raspberry colored, with a dubious (+) - it contained traces of the formation of the color of raspberry jelly. To detect latent mastitis in sheep, we proposed the rapid test of the A1 test, which is an aqueous solutio...

Kamilla Gadzhimuradovna Alieva1, Patimat Mitkhatovna Daniyalova1, Svetlana Aleksandrovna Shemyakova2*, Kerim Khasanovich Bolatchiev2, Ismail Anatolyevich Bittirov3 

.... Terek, R. Malka, R. Baksan, R. Cherek, R. Chegem, R. Gizhgit, R. Kendelen, R. Kichmalka, R. Khaznidon, R. Sukan-su.at 12.00-30.0% of the samples, eggs of Trichocephalus ovis (Abildgaard, 1795) and Trichocephalus skrjabini (Baskakow, 1924) were found. Study of the dynamics of soil pollution by eggs of the genus Trichocephalus (Schrank, 1788) in the mountainous zone in 2017–2020. revealed moderate pollution of water an...

Vetlana Aleksandrovna Shemyakova1, Boris Kazievich Laipanov1, Ismail Anatolyevich Bittirov2, Kerim Khasanovich Bolatchiev1, Kamilla Gadzhimuradovna Alieva4, Anatoly Murashevich Bittirov2,3* 

...) 60.00 - 90.00% of soil samples are contaminated with eggs of the nematodes genus Bunostomum (Raillet, 1902) (average 13,80±1,30 ekz. per 5 g of soil). The dynamics pasture of soil contamination in the mountain zone with eggs of nematodes of the genus Bunostomum (Raillet, 1902) was characterized of by an increase in pollution soil samples from 62,00 to 95,00% (on average by 77,60%), which indicates the like...

Aiman Al-Mufarji, Shaker Al-Suwaiegh, Abd El-Nasser Mohammed*

... 21. Collection of blood samples from ewes and lambs were performed at -8 weeks and -4 weeks pre-partum, parturition, +4weeks, and +8weeks postpartum. The blood samples were analyzed for blood and metabolic profiles, liver enzymes, and minerals. The results indicated that ewes suffered from thermal stress during the study and the stress was alleviated due to M. oleifera supplementation. The body weight of ewes (p<0.05) an...

Roni Pazla1, Novirman Jamarun1*, Elihasridas1, Arief1, Gusri Yanti2, Zaitul Ikhlas2

... corn, palm kernel cake, salt, and minerals. The treatments were as follows: T0= 35% fermented sugarcane shoots (FSS) + 5% fermented tithonia (FT) + 0% avocado waste (AW) + 60% concentrate, T1= 35% FSS + 9.5% FT + 0.5% AW + 55% concentrate, T2= 35% FSS + 14 % FT + 1% AW + 50% concentrate, T3= 35% FSS + 18.5% FT + 1.5% AW + 45% concentrate. The parameters measured included feed consumption (dry matter, organic matter, and crude protein), nutrient digestibility ...
Seyedmohammad Karimi1, Mohammad Darvishi2*, Mohammad Barati3, Ramin Hamidi Frahani4, Mohammad Hassan Kazemi5
...ients with Covid-19 in Besat Hospital, Tehran, Iran, during 2022, were enrolled. Patients were randomly divided into two groups of 35 each. Both groups were administered Remdesivir with same protocol. In this period, the first group received N-acetylcysteine 600 mg tablet twice daily and melatonin 6 mg tablet at bedtime. The second group received placebos with the same appearance. Liver en...

Muhammad Humayun Kabir* and Md. Saiful Islam

...th purposive and random) sampling method was applied to select the study location as well as the sampling unit (farmer/household head). Data were collected from randomly selected 336 farmers of Dhamrai Upazila (sub-district) under Dhaka district, Bangladesh using a face-to-face interview survey. Focus Group Discussions (FGDs) were also conducted to cross-verify the collected data. The data were analyzed using some common des...

Aqleem Abbas1, Mustansar Mubeen2, Yasir Iftikhar2, Qaiser Shakeel3*, Hafiz M. Imran Arshad4, Maria del Carmen Zuñiga Romano5 and Sarfaraz Hussain6

... crops worldwide. The causal agent of RSB is the necrotrophic fungus Rhizoctonia solani. Finding appropriate management strategies to combat the disease to minimize rice yield losses and reduce global food security threats is challenging. However, scientists are still figuring out the best way to safeguard rice crops from RSB. Recently various pathogenesis-related (PR) viz. PR-3, PR-5, PR-9, PR-10, PR-12, and PR-13 and WRKY ...

Tariq H.Y. Al-Maliky*, Mahmood S. Hashim and Amal S. Al-Sheraa

...e shrimp cultured in two salinities (3 and 15 g.L-1) were observed during 45 days. The initial and final weights of M. affinis were (0.939 ± 0.069 and 1.581 ± 0.183 g) in 3 g.L-1, and (0.939 ± 0.065 and 2.274 ± 0.294 g) in 15 g.L-1. While for M. nipponense, it was (0.914 ± 0.086 and 1.308 ± 0.070 g) in 3 g.L-1, and (0.908 ± 0.070 and 1.086 ± 0.107 g) in 15 g.L-1. The environmental factors were temperature...

Ahmad-ur-Rahman Saljoqi, Sadar Iqbal and Imtiaz Khan*

...s Neem Oil. Herefore, by sacrificing some yield of brinjal the Neem oil is recommended as an environment friendly management technique for brinjal shoot and borer.

...

Syed Shah Mohioudin Gillani1, Muhammad Naveed Tahir1, Adeel Anwar1*, Syed Ijaz Ul Haq2, Muhammad Awais3, Mujahid Iqbal4, Javed Iqbal5, Hina Ahmed Malik3, Syed Muhammad Zaigham Abbas Naqvi6, Raza Ullah7 and Muhammad Abdullah Khan1

...acity of high-resolution satellite imagery to estimate wheat chlorophyll content coupled with ground-based information for accurately monitoring crop chlorophyll status under rainfed conditions. Images with zero clouds from LANDSAT 8 and ground data collection were carried out in a simultaneous time frame. Vegetation indices (VI) comprised of normalized difference vegetation index (NDVI), ...

Muhammad Umair1,2, Muhammad Naeem1, Asad Jamil3 and Muhammad Younas2,4*

...xt-align: justify;">Soil salinization is a global issue regarding decline in productivity of agricultural fields. It is the major abiotic factor behind loss of crop production in arid to semi-arid zones. High temperature and low rainfall lead to accumulation of salt on the soil surface. Moreover, the low organic matter status of soils further worsens the soil conditions. All these factors have severely reduced soil productiv...

Anatoly Murashevich Bittirov1*, Sadrutdin Shamshitovich Kabardiev1, Ayub Yusupovich Aliev1, Boris Kazievich Laipanov2, Svetlana Aleksandrovna Shemyakova2, Ismail Anatolyevich Bittirov1  

...(the village of Upper Baksan, the village of Elbrus, the a/l Dzhantugan, the village of Tegenekli, the village of Baidaevo, the a/l It-kol, Cheget, Terskol, Azau) in the populations of the Mountain Caucasian ground squirrel, 12 cestode species: Raillietina sp., Rodentolepis straminea, Paranoplocephala transversaria, Rodentotaenia bondarevae, Mathevotaenia symmetrica, Paranoplocephala omphalodes, Skrjabinotaenia lobata, Paran...

Khasan Khamidovich Shakhbiev1*, Saidkhamzat Saidmagomedovich Abumuslimov1, Larisa. Vakhaevna Taramova1, Islam Khasanovich Shakhbiev1, Svetlana Aleksandrovna Shemyakova2 

...ch the is a biologically safe, effective therapeutic and prophylactic agent for echinococcosis of young of dogs complicated by streptococcosis. For the first time, results have been obtained confirming that the components of azithroprazifene (praziquantel, azithromycin, fenbendazole, copper chelate, cobalt chloride and zeolite) not only had an etiotropic and pathogenetic effect against causative agents of echinococcosis and ...

Mishal Bano1, Amir Shakeel1, Waqar-ul-Haq1, Muhammad Nouman Khalid1*, Nadia Hussain Ahmad2, Muhammad Sajid Sharif3, Sadia Kanwal2, Muhammad Asim Bhutta2, Amna Bibi2 and Ifrah Amjad1

...ersity of Agriculture Faisalabad using two replications with randomized complete block design (RCBD). Results of ANOVA revealed that all the genotypes were highly significant for most of the plant height, sympodial branches/plant, monopodial branches/plant, first fruiting nodes, number of bolls per plant, boll weight, number of seeds per boll, seed cotton yield/plant, lint index, lint percentage, seed index, seed density, seed volume, fiber fineness, fiber str...

Kainat Bibi1*, Sajjad Khan1, Zulfiqar Ali Gurmani1, Fahad Karim Awan1 and Sajid Ali2 

...ultivars (Atta Habib, Faisalabad, ASS, Punjab 2011, Lasani, NARC-11 and Pakistan-13) were sown using randomized complete block design (RCBD) with three replications. Source of organic manure was compost, poultry manure and farm yard manure while for inorganic treatments commercial fertilizers urea and di-amonium phosphate fertilizers were applied at recommended doses. Data was recorded on various morphological, yield and qua...

Ayaz Ahmed1*, Muhammad Zulfiqar1 and Saadutullah Khan2

... and from those villages sample size of 321 households was selected randomly. In the study areas majority (45%) of sample households had 5 to 10 family members which indicated joint family system. Literacy status in study areas was very low (30%). Sayed, Swati, Awan, Gujjar and Kohistani tribes were residing in the study villages. Crop production was their primary occupation, secondary livestock rearing; tertiary

Saba Iqbal1, Salman Khurshid1*, Hafiza Mehwish Iqbal1, Qurrat-Ul-Ain Akbar1, Aqeel Ahmed Siddique3, Saqib Arif1, Shahid Yousaf2, Masooma Munir4, Abdul Karim Khan4, Shazia Arif5,  Abdul Ahad6 and Muhammad Arif7

...ty of NIR for variety of samples matrices. The present work has been undertaken to assess the efficacy of NIRS method for quantitative characterization of wheat grains in terms of hardness. Since wheat is the staple food and its hardness is one of the basic quality traits being used in local and international trade. In addition to the NIR-spectroscopy, wheat grain samples were also analyzed through standard methods for hardn...

M.M. Mikailov1*, O. Yu. Chernykh2, E.A. Yanikova1, G.A. Nurlygayanova3, O.D. Sklyarov4 

...e-linked immunosorbent assay (ELISA), agglutination reaction (RA), complement fixation reaction (CSC), rose-bengal test (RBP) and immunodiffusion reaction with O-PS antigen (RID with O -PS antigen). The specificity of these serological tests was confirmed by the negative results of studies of 40 blood serum samples from animals from a

Faustin Dokui1*, Frédéric M. Houndonougbo1, Service G. Djidda1, Venant P. Houndonougbo1, Edith Gangbedji1, Gwladys Menon Agbo2, Sedjro Ludolphe Dedome2, Séverin Babatoundé1, Soumanou Seibou Toleba1, Christophe A.A.M. Chrysostome1 

...treatment group fed with salt-poor stone during the experiment period. Cows in the LS2 treatment group produced much more milk during the dry season than the other treatment groups. Cows in the LS3 treatment group fed with stone low in salt produced much more milk from the fifth week to the end of the trial than the other treatment groups. Animals in the control group lost significant body weight. Thus, supplementation of li...

Mutia Rizkia Shaffira, Nurhayati, Dwi Desmiyeni Putri* 

...o, and mortality. Twenty samples were used to measure the observed variables in carcass quality including carcass percentage, abdominal fat, cholesterol, and cooking losses. The results showed that the addition of red ginger and brotowali extracts in drinking water both single or in combined application improved on body weight gain, conversion ratio, mortality, carcass percentage, abdominal fat, and cooking loss significantly (p<0.05). Administration of a c...
Safika1*, Ni Luh Putu Ika Mayasari1, Juliadi Ramadhan2
...This study’s total sample comprised of 58 clinical cat laryngeal swabs. Samples were isolated and biochemically and molecularly identified. The Kirby-Bauer disk diffusion method was used to test positive isolates, followed by PCR method to discover the resistance coding genes. The results showed that ampicillin showed 76.0% resistance to Klebsiella pneumoniae, followed by oxytetracycline (72.0%) and tetracycline (68.0%), enrofloxacin (52.0%), and gentami...

sans-serif; background-color: rgb(255, 255, 255);">A.W. Amin, Abd-El Wanis, Mona, A. Rahman and Tomader

J. Salma and F. Shahina

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">Eight nematode species of the genera sans-serif; background-color: rgb(255, 255, 255);">Steinernemasans-serif; background-col...

sans-serif; background-color: rgb(249, 249, 249);">S. A. Khanzada, M. Naeemullah, A. Munir†, S. Iftikhar and S. Masood

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">Thirteen mint species were evaluated for the presence of nematode fauna associated with their rhizospheres. Six plant parasitic and six saprophytic nematode genera were found associated with mint rhizosphere. sa

sans-serif; background-color: rgb(255, 255, 255);">A. N. Mahar, N. D. Jan†, A. Q. Mahar and S. R. Gowen

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">Entomopathogenic nematodes sans-serif; background-color: rgb(255, 255, 255);">Steinernema sans-ser...

sans-serif; background-color: rgb(249, 249, 249);">M. A. Radwan†, S. A. A. Farrag, M. M. Abu-Elamayem and N. S. Ahmed

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">Five granular nematicides namely, cadusafos, carbofuran, ethoprop, fosthiazate and oxamyl were assessed against the root-knot nematode, sans-serif; background-color: rgb(255, 255, 255);">Meloidogyne incogni...

sans-serif; background-color: rgb(249, 249, 249);">B. K. Goswami, C. Bhattacharya, R. Paul and T. A. Khan

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">A microplot experiment was carried out for assessing the performance of fungal biological control agents and chemical pesticides/nematicides on growth, yield and forskolin content in root-knot nematode infected sans-serif; background-color: r...

sans-serif; background-color: rgb(249, 249, 249);">A.E. Ismail and M. M. Mohamed

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">Three animal culture manures viz., cattle manure (CM), sheep manure (SM) and chicken manure (ChM) at three rates as organic substances, incombinination with urea as inorganic fertilizer, were tested for their action against root-knot nematode, Meloidogyne arenaria infesting sugar beet and plant growth, yield and total soluble sugars (TSS %) under new reclaimed ...

sans-serif; background-color: rgb(255, 255, 255);">M. A. Haq, M. Shahid and K. Mahmood

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">In a field trial at area of Vegetable Research Institute (AARI), Faisalabad in 2011, a new host of root- knot nematode (RKN) was recorded. Moderate galls on the roots of sans-serif; background-color: rgb(25...

sans-serif; background-color: rgb(249, 249, 249);">W. Khan, N. U. Nisa, A. Khan and S. M. H. M. Naqvi

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">This study was aimed to assess the impact of education on the prevalence of nematode intestinal parasites among individuals relevant to education under and above 15 years age in Swat, Pakistan. Stool samples were randomly collected during January 2006 to December 2008 and examined from a total of 420 individuals including 238 and 182 under and above ...

sans-serif; background-color: rgb(249, 249, 249);">A. Khan, B. Nawab, S. S. Shaukat, M. A. Samad and J. K. Tareen

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">The effect of Fertinemakil-Plus and Carbofuran was investigated on the population densities of three nematodes and yield of chilli. The populations of sans-serif; background-color: rgb(255, 255, 255);">Meloidogyne incognita

sans-serif; background-color: rgb(255, 255, 255);">D. S. Srivastava, M. Sehgal†, A. Kumar, S. Verma, B. K. Dwivedi and S. P. Singh

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">An extensive survey was carried out for the infestation of plant parasitic nematodes associated with vegetable growing fields i.e., tomato and okra. Soil and root samples were collected from 238 tomato fields represents 16 different locations (villages) where TSS-1 to TSS-238 varieties and 227 soil and root samples...

sans-serif; background-color: rgb(249, 249, 249);">B. Archana and R. Saxena

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">In the present study aqueous extract of medicinal plants viz., Amaranthus spinosus,Chenopodium album, Catharanthus roseus, Solanum nigrum and Ocimum sanctum were investigated for their nematicidal activity against second stage juvenile of Meloidogyne incognita by in vitro technique. Aqueous extract of all plant roots exhibited nematicidal activity. L...

sans-serif; background-color: rgb(255, 255, 255);">O. A. Fabiyi, G. A. Olatunji and O. Atolani

...quot;, Helvetica, Arial, sans-serif; background-color: rgb(255, 255, 255);">The leaves of sans-serif; background-color: rgb(255, 255, 255);">Alstonia booneisans-serif; background-color: rgb(255, 255, 2...

Saima Akter1, Md. Rasel Prank1, Shariful Islam1, Sharmin Akter1, Injamamul Hasnine1, Murshed Uddin Ahmed2, Md. Shohel Al Faruk3* 

...sh. A total of 121 fecal samples were collected and examined through a direct smear to detect the presence of the egg and oocyst of parasites. The overall prevalence of cattle parasitic infestation (mixed and single) was 80.17% (N=97/121). The overall prevalence of trematode was 49.58%, nematode was 57.86%, and protozoa was 9.1%. The infestation rate of the Strongylys group (45.4%) was dominant followed by Fasciola gigantica (24.79%), Paramphistomum spp. (24.7...

Tae-Kyung Eom1, Jae-Kang Lee1, Dong-Ho Lee1, Ho-Kyoung Bae1, Hyeongyu Ko1, Kyu-Jung Kim1, Sung-Cheol Jung2, Jong-Hwan Lim2 and Shin-Jae Rhim1*

...us caudatus in the Seoraksan and Jirisan National Parks, South Korea. We compared activity patterns among pairs of sympatric species in each area, as well as those of water deer and roe deer populations inhabiting the two parks. Overlaps in activity patterns were estimated based on kernel density function and overlap coefficient analyses, and activity pattern distributions were compared using Watson’s two-

Hidayat Ullah1, Sadia Tabassum1, Sultan Ayaz2, Shumaila Noreen1, Atta Ur Rehman1, Naveed Akhtar3, Muhsin Ali3, Zaib Ullah3* and Sajid Mahmood1,4

...s, Rhipicephalus haemaphysaloides, Haemaphysalis bispinosa were identified from goats and three species Hyalomma marginatum, Hyalomma excavatum, Rhipicephalus microplus from sheep. Goat breed wise infestation rate was recorded in Kaghani, Kamori, Sindhi, Lehri, Barbari, Beetal and Dera Din Panah. In sheep breeds infestation rate was recorded in Gauder, Balkhi Afghani and Rambouillet. On ba...

Mohamed S. Abbas1*, Adel E.M. Mahmoud2, Hemat S. Mohamed3, Adam Cieślak4 and Małgorzata Szumacher-Strabel4

...NH3 and methane in Vicia sativa and TGP in Trigonella maritime. Whereas, in forbs the highest concentration of pH was found in Periploca angustifolia, NH3 in Anacyclus nummularia and TGP and methane in Anacyclus alexandrinex. From among grasses: Aegilops kotschyi had the highest value of acetic, propionic, VFA, APB (acetic, propionic, butyric) and A/P (acetic to propionic ratio) and Aeluropus lagopoides had the highest value of isobutyric, butyric, isovaleric ...
Aadil Sultan, Mohsin Ahmad Khan*, Nadeem Ahmed, Muhammad Hassan, Rashid Bhatti, Hafsa Naeem, Muhammad Islam Khan, Saad Tahir,  Samia Afzal* and Ahmad Ali Shahid
...enetic factors is gene dosage, which can be increased by increasing the vector copy number. However, increased number of expression vector poses some metabolic burdens on bacterial cells. The current study provides gene multimerization strategy as an alternate way to amplify the gene dosage and explores its effect on the expression of human epidermal growth factor (hEGF) by constructing three types of expression vectors i.e....

Rehman Shabbir1, Ghulam Sarwar1, Noor us-Sabah1, Mukkaram Ali Tahir1, Muhammad Luqman*2, Muhammad Fahad Ullah3, Muhammad Zeeshan Manzoor1 and Imran Shahzad1

...ith canal water that can safely be used for getting good quality tomatoes and to mitigate hazardous impact of heavy metals contained in wastewater. 

...

Esam A Razin1, Hassan Sobhy2, Tarek R. AboElnaga1, Asmaa A. Darwish1*, Rasha S. Mohammed1

...th, and 28th days. Blood samples were collected daily for monitoring the parasitemia and the clinicopathological parameters were measured at 0 and 49 days. Tissue sections were obtained from the liver, kidney, heart, lung, spleen, and brain on the 49th day, stained, and histopathologically examined. Although, both treatments presented a significant improvement (P<0.05) in their parasitological and clinicopathological results, DAG suffered from lower levels ...

Hesham A. Mahran1,2*, Shawky M Aboelhadid3, Saad S. Alqahtani4,5, Mohamed Z. Sayed Ahmed4,5

...rent study was to create safe, antibacterial formulations based on cinnamon oil that were effective against Culex pipiens adults and pupae. Cinnamon nanoemulsion (CNE) was synthesized and characterized. The binary mixture of cinnamon with sesame oil (CS) was prepared at a rate of one part cinnamon to 3 parts sesame oil. Multiple concentrations from these formulations were prepared and test...

Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6

...ial examination of 200 nasal swabs and 30 lung tissue samples (taken from 30 emergency slaughtered cattle) indicated that Klebsiella spp. was recovered in 55/230 (24%) of all samples with a high proportion of K. pneumoniae (18.7%). In-vitro antibiogram exhibited a wide MDR phenotype. The virulence genes (fimA, aerobactin, rmpA and magA) were exhibited in 90%, 85%, 90% and 80% of the tested...

Abdul Azis*, Afriani Afriani

...1 days. The weight of bursa Fabricious increased (P<0.05) with the feeding time restriction at age 14 days. However, there were not significant different in all of parameter at the end re-alimentation period (42 days). Based on our findings, feeding time restriction in broilers by 4 hours/day had no negative effect on body weight at slaughter age, carcass characteristics, or gastrointestinal and immune organs.
 
Keywords | Feed ...

Trisiwi Wahyu Widayati1*, Aris Triyono Syahputra2, Andoyo Supriyantono1, Onesimus Yoku1, Deny Anjelus Iyai1, Priyo Sambodo1, Iriani Sumpe1 

...cation of laleken. Stool samples come from fresh feces. Water samples were collected from pools around the farmer’s house in both systems, vegetable samples were collected from three points ± 0 m, ± 1 m, and ± 2 m apart from the fence and soil samples were collected at three points ± 1 m apart, ± 10 m apart, &plus...
Ferzana Bhatti1, Fiaz Hussain2, Zain ul Abdin1*, Muhammad Arshad1, Saqi Kosar Abbas2 and Muhammad Zeeshan Shabbir3
...ptera) was studied. Bioassays were performed with microinjections carrying crude and diluted venom and secretion of Dufour’s gland of B. hebetor into healthy and mature host larvae. To observe the effects of venom injection on development of parasitoid progeny, freshly laid eggs of the parasitoid were transferred from stung hosts onto non-stung hosts. Venom caused 100% mortality of the host even in diluted forms; Dufour’s gland secretion did not ca...

Bilgenur Yasa1* and Ali Uzun2

...Karacabey District of Bursa Province, in a study conducted over the course of one year (2017). The distribution of species in terms of orders is as follows: Falconiformes, 1 (0.8%); Phoenicopteriformes, 1 (0.8%); Bucerotiformes, 1 (0.8%); Galliformes, 1 (0.8%); Cuculiformes, 1 (0.8%); Caprimulgiformes, 1 (0.8%); Coraciiformes, 2 (1.6%); Gruiformes, 2 (1.6%); Podicipediformes, 2 (1.6%); Suliformes, 2 (1.6%); Pelecaniformes, 3 (2.5%); Strigiformes, 3 (2.5%); Col...

Songruo Tao1, Cuiyi Liao1, Jinju Peng1, Yuexia Ding1,* and Yi Ma1,2,*

...ere used to collect soil samples on the 7 d, 21 d and 49 d after dosing. The effects of florfenicol on 16S rDNA sequence diversity of soil phosphorus-solubilizing bacteria were determined by amplifed ribosomal DNA restriction analysis (ARDRA) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) method. The results showed that the number of Operational Taxonomic Units (OTUs) types decreased with the increase of florfenicol co...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5 

...nt of reference was necessary to achieve this aim. The semen samples from Bali-horned and Bali-polled bulls were obtained twice weekly using an artificial vagina. The semen samples were immediately sent to the laboratory for processing. Parameters measured were sperm motility, abnormality, viability, acrosome integrity, and membrane integrity. Liquid chromatography-mass spectrometry (LCMS/...

Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1 

...s, and apoptotic TUNEL assay, were evaluated after three weeks in addition to Immunohistochemical analysis was done on the proliferating cell nuclear antigen (PCNA), hypoxia-inducible factor 1-alpha (HIF-1), PPAR, and cyclin D1. Results: Mice treated with Cis+Pio HD showed higher levels of SOD, GSH, p-ERK1/2 and p38-MAPK, as well as lower levels of MDA and tumor growth factor beside the higher apoptotic activity. Furthermore, Cis+Pio HD reduced the expression ...

Safriyanto Dako1, Nibras Karnain Laya1, Syukri I Gubali1, Ari Ardiantoro2, V.M Ani Nurgiartiningsih3, Gatot Ciptadi3, Desinta Wulandari3, Suyadi Suyadi3*

...xt-align: justify;">Microsatellites are one option for characterizing cattle populations; that information can be used as a basis for the development and maintenance of local cattle. This study aims to evaluate genetic diversity and relationships using microsatellite markers between the populations of Gorontalo cattle in Gorontalo Province, Indonesia, using 3 microsatellite markers. A tota...

Noor Rahmawati, Alfi Rumidatul* and Sopandi Sunarya

... and Pseudomonas aeruginosa were used. The experiment showed that the addition of yeast and sengon wood to PDB medium increased the growth and production of bioactive compounds extracted from fungal cultures. A fungal endophyte extract in ethyl acetate from all media used produced antioxidant compounds with high activity. Antibacterial activity was obtained from the extract of fungal cultures grown on PDB plus Sengon wood floor on day 6 for P. aerugino

Saleem Hussain1, Muhammad Tayyab1, Tauqir Anwar1, Talha Nazir2*, Muhammad Zeeshan Majeed3, Zohaib Asad2,4, Muhammad Adnan3 and Tajwar Alam5

...ay method, toxicity bioassay was conducted with three different concentrations (i.e. 250, 500 and 1000 ppm) of methanolic plant extracts under controlled laboratory conditions, while three different concentrations (25, 50 and 75 g/L) of each botanical extract was tested in the field trial. Results showed a significant mortality and suppression of S. graminum individuals at 72 h after treatment in the laboratory and at 10th day post-treatment in the field. In l...

Nasratullah Baloch1*, Naeem Tariq Narejo2, Hamida Narejo3, Muhammad Farooq Hassan4, Muhammad Hanif Chandio5, Faheem Saddar6, Dharti Shahnawaz Thebo7, Ghulam Abbas8 and Shahnaz Rashid8

...ixed with 5% formalin in sampling bottles. The 99 genera of both the planktons were recorded, which included 52 zoo- and 47 for phytoplankton from the experimental area. Among the zooplankton, Copepods were found to be rich 65% followed by Brachyuran zoa 15% while in case of phytoplankton genus Coscinodiscus were noted to be rich 60% followed by Rhizosolenia 15% during the whole duration of investigations. It reflects that the bay water was found to be rich wi...

Ali I. Al-Ameedi1, Zahraa M. Ayad2*, Wed Abbas Mohammed3, Salim K. Hajwal4

...ide preparation with Giemsa stain, experimental animals’ chromosomal abnormalities, mitotic index, and blast index were examined. In order to ascertain the protective activity of the plant extract, the mitotic index and blast index are also assessed. The results showed that the exposed group (T1) had a significantly lower mitotic index (the number of cells in mitosis per 1000 bone marrow cells) than the control group (which only drank distilled water). T...

Muhammad Yaseen1, Muhammad Luqman1*, Sheer Abbas2, Usman Saleem1, Abdul-Gafar Ahmed3 and Muhammad Salman Aslam1

Abdirahman Barre*, Karanja D Njuguna, Bebora Lilly Caroline and George Chege Gitao

... slaughterhouses in Garissa County, through serological testing and pathological lesions that encountered at post mortem inspection of camel meat. Three sub-counties; Garissa Township, Dadaab and Balambale were purposefully recruited based on presence of camel slaughterhouses and accessibility. A hundred and sixty (160) camels were selected from 238 presented during the visits based on clinical manifestations suggestive of B...

MARIUM ASLAM, MUHAMMAD JAVED, FAIZA AMBREEN* & FARIHA LATIF

...er 30-day, the fish were sacrificed and their liver and kidney were analyzed for peroxidase enzyme activity. Change in activity of peroxidase enzyme in the fish exposed to sub-lethal concentrations was compared with the control. Dose dependent increase in the activities of peroxidase enzyme was observed in the tissues of fish as compared to the control group. Peroxidase activity in the liver of fish from all the treatments was significantly (p<0.05) higher ...

ASIMA BANO1, HAFIZ MUHAMMAD TAHIR*2, AROOSA RASHEED3, MUHAMMAD ARSHAD4, SHAFAAT YAR KHAN1 & RABIA YAQOOB5

...atients. For study blood samples were collected from various diagnostic laboratories of Sargodha city. Levels of non-specific esterases were determined in the normal and breast cancer patients. The levels of non-specific esterases were higher in healthy individuals than the breast cancer patients. So, levels of non-specific esterases could be used as a marker for the diagnosis of breast cancer.
 
Key Words: Esterases, breast cancer...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

...n were surveyed and leaf samples showing typical symptoms of citrus canker were collected. Infected section of leaf was taken for isolation of bacteria. Lesions were cut into parts and streak bacteria by the help of inoculation loop grown in nutrient medium. Pure bacterial culture of Xanthomonas axonopodis were used for detection by standard PCR. Xanthomonas axonopodis was diagnosed by amplification of 16S rDNA. A fragment of ~1.4 kb was amplified and cloned f...
ASMA ABDUL LATIF*1, SAMREEN MUSHTAQ1, SABIHA FAZAL1, MUHAMMAD MANSHA2, & ATIF YAQUB3
...n.
239 blood samples of pregnant women were collected from Lady Walingdon hospital, Lahore along with other details on associated risk factors of infection. From these samples, 86 were randomly selected to be evaluated for the presence of IgG immunoglobulin by using enzyme-linked immunosorbent assay (ELISA)...

MUHAMMAD EJAZ*1, MUHAMMAD JAMIL YOUSAF5, ASMA MAQBOOL3, ALTAF HUSSAIN4 & ABDUL QAYYUM KHAN SULEHRIA2

MUHAMMAD ASHFAQ*1, QURAT-UL-AIN ASGHAR1, RAHILA HAFEEZ1, AMNA ALI1, MUHAMMAD SALEEM HAIDER1, MUHAMMAD ALI1, MUHAMMAD BILAL CHATTHA1 ABDUL RASHEED1 & MUHAMMAD SAJJAD2

...lora of five rice (Oryza sativa L.) varieties viz. Basmati-198, Basmati-802, Basmati Pak, Basmati Shaheen and Basmati-370 were studied using agar plate and blotter paper method. Highest percentage of myco-flora (17%) was found to be associated with seeds of Basmati Pak that was considerably more than myco-flora associated with all other varieties of rice. The highest percentage of bacterial isolates (16%) was recorded with Basmati Shaheen variety. Total of six...
TAHIR MEHMOOD*, ANUM AFZAL, ABDUL KARIM, MUHAMMAD ASHRAF SHAHEEN &
HINA ARSHAD
...tivity, reducing power assay and ABTS (radical scavenging activity) of different solvent extracts, from Citrus limon (unripe and ripened) peels. In unripe peels the total flavonoid contents, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power ranged from 18.23-57.56 mg/g CE (catechin equivalent), 21.69-65.82%, 38.96-84.83% and 0.01-1.8 mg /mL, respectively. For ripened lemon peels all these indicators ranged from 10.53-47.88 m...
SYED ZULFIQAR ALI ABIDI, HAFIZ ABDULLAH SHAKIR, SYED SHAHID ALI, & JAVED IQBAL QAZI*
...nnine, free amino acids, saponins, tannins, terpenoids, quinine, oxalic acid and steroids). Twenty one plants showed the presence of phytochemicals. The use of piscicidal property of these plants may improve the production of aquaculture by getting rid of unwanted fish species.
 
Key Words: Alcoholic extract, Aqueous extract, Phytochemicals, Metabolites, Plants
...
SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
...hi; a total of 175 water samples were collected from the study area with 35 samples from each source. All the samples were analyzed for microbial contamination by pour plate method, membrane filtration technique and MacConkey agar for the isolation of Enterobacteriaceae. Different identification tests have been carried out by using various selective and differential media (MacConkey agar, ...

Subramaniyam Suresh, Saravanakumar Marimuthu*, Prashanth D’Souza 

...erase (AST), alanine transaminase (ALT), and gamma-glutamyl transferase (GGT). Results revealed that 4% FCM was numerically improved on week 1 (0.11), week 2 (0.46), week 3 (0.56), and week 4 (0.54) as compared to baseline in PTF supplemented group. Milk fat (%) was also significantly (p < 0.001) improved on weeks 2, 3 & 4 as compared to baseline in PTF supplemented group. Serum total cholesterol (p < 0.001) and triglycerides (p < 0.05) levels wer...

Chun Ik Lim, Hyeon Kwon Kim, Kang Nyeong Heo, Are Sun You, Hyo Jun Choo* 

...ietary groups: 1) CON, basal diet; 2) FGP0.5, basal diet with fermented garlic powder at 0.5 g/kg; 3) FGP1.0, basal diet with fermented garlic powder at 1.0 g/kg, and 4) FGP2.0, basal diet with fermented garlic powder at 2.0 g/kg. FGP addition had no significant effect on egg production (EP) or egg weight (EW). However, eggs from groups fed a diet supple...

Nguyen Thi Kim Dong1*, Nguyen Van Thu2 , Nguyen Hoang Qui3 

...Four diets included a basal diet (SBP0), which consisted of fish meal 20% (FM), broken rice 80% (BR) and the other three diets, in which the content of FM was 25.0, 50.0 and 100% of the FM level in the basal diet and was replaced by ensiled shrimp by-products corresponding the SBP1, SBP2 and SBP3 diet, respectively. Daily intakes of ether extract (EE) and fiber fractions increased with increasing levels of SBP. The apparent...
 
 SADAF SARFRAZ1*, SAFDAR AMEER1, REENA AMBREEN1, SHOMAILA SIKANDAR 2, SHAISTA NAWAZ3 AND YASIR SALEEM
... was found that cinnamon samples collected from Sri Lanka has the highest oil content (3.1%), followed by China (2.8 %) and India (1.9 %), where all tests were standardised using fixed oil levels. In terms of antimicrobial properties, obtained results demonstrate that Indian cassia (Cinnamomum tamala) exhibited the highest antimicrobial activity as compared to the samples co...
 
 SAIMA AFTAB1, ATTIA HAMID1, MAHJBEEN2, JAVERIA TARIQ2, ZULQURNAIN ALI2, ASMA ZAFAR2* 
 
 SAIMA AFTAB1, ATTIA HAMID1, MAHJBEEN2, JAVERIA TARIQ2, ZULQURNAIN ALI2, ASMA ZAFAR2* 
 
 ANSA KHALID, UZMA HAMEED* AND IKRAM-UL-HAQ 
...tables, fruits, and milk samples collected from the local market of Lahore, Pakistan. Sucrose-based medium, supplemented with vancomycin and tetracycline, was used for the selective growth of Leuconostoc species. The obtained isolates were screened by using the shake flask method. The isolate IIB-C9 was selected for the parametric optimization as it gave the maximum activity. Among the 10 different fermentation media screened for enzyme production, maxi...
 
 ANSA KHALID, UZMA HAMEED* AND IKRAM-UL-HAQ 
...tables, fruits, and milk samples collected from the local market of Lahore, Pakistan. Sucrose-based medium, supplemented with vancomycin and tetracycline, was used for the selective growth of Leuconostoc species. The obtained isolates were screened by using the shake flask method. The isolate IIB-C9 was selected for the parametric optimization as it gave the maximum activity. Among the 10 different fermentation media screened for enzyme production, maxi...
 
 MONIS HUSSAIN SHAH1*, RIAZ UR REHMAN2, RIAZ ALI SHAH1, RIZWAN RAFIQUE3, MUHAMMAD USMAN4, SAJIDA BIBI5 AND SADIA YASEEN
... from gerbera cut-flower sale. The standardization of suitable substrate for extending vase life of gerbera is not present. The gerbera was placed in substrates 6-Benzyleadenine, Silicic acid and Silver nitrate (20, 40, 60, 80 and 100 ppm) with sucrose (5, 10, 15, 20 and 25g), respectively. The cut flower response was observed by observing the time (days) for sustaining the freshness of cut flowers. Net fresh weight (g) after 15 days according to the formula a...
 
 MUHAMMAD MUDASSAR SHAHZAD1*, FATIMA KHALID1, MEHWISH FAHEEM2, SYED ZAKIR HUSSAIN SHAH3, SANA BASHIR1 & SYED MUHAMMAD KHAN
...ity minerals occurred on said level. Our results showed that Tau could be used as a feed additive in linseed meal based diet to confer improved health status and higher growth in common carp at a 4 g/kg level in a plant-based diet. 
...
 
 USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF
... Psyllium, a natural polysaccharide, is a dietary fiber and widely utilized in gel forming. To study the structural aspects of these various polymeric webs were categorized with FTIR, SEM and release related experiments such as swelling kinetics, swelling ratio and drug kinetics and the impact of pH on the release of insulin from medicated hydrogels has been concentrated to evaluate the medication discharge instrument in-vitro and in-vivo. Fickian diffusion me...

Arief1, Roni Pazla2*, Rizqan1, Novirman Jamarun2 

...versifolia plants and cassava leaves have the potential to be used as a substitute for field grass. Palm kernel cake is a waste of the palm oil processing industry and can be used as a concentrated alternative. This study aims to determine the impact of giving Tithonia diversifolia plants, cassava leaves, and palm concentrates on the milk production and quality (fat and lactose), intake, and digestibility of Etawa Crossbred ...

Ahmed Abdel-Rady3*, Mohamed Karmi2, Menna_allah Youssef1, Aml M. Abdel-Ra’ouf1, Bahaaa Madkour1 

...ate was 56 (21.13%). Giemsa stained blood smears showed presence of macro-schizont inside lymphocyte (Koch’s blue bodies), micro-schizonts inside lymphocyte, raptured schizont and intraerythrocytic stages of Theileria annulata piroplasms inside RBCs..Polymerase chain reactions of T. annulata merozoite-piroplasm surface antigen Targeting gene: (Tams1) revealed positive 29 (58%) animals confirmed by visualization of specific bands at 768 bP. Positive resul...

AZKA ASIF, HAREEM MOHSIN*, SADIA N CHAUDHRY, & WARDA FATIMA

... are several screening assays available for screening and evaluating the presence of biosurfactants but many of these show variable results under the same conditions and for the same organism. Most commonly applied screening assays including oil spreading technique (OST), tilted glass slide assay (TGS), drop collapse a...

SAIMA SHARIF*1, SHAGUFTA NAZ1, RIDA IRAM1, FARKHANDA MANZOOR1, TASNIM FARASAT1 and MUHAMMAD SAQIB2

...ikhupura. A total of 500 samples of children were collected. Questionnaire was designed to collect information for studying variables regarding age, gender, father‟s occupation, parent‟s education, child‟s family income etc. Their blood samples were collected and analyzed to find out the level of Haemoglobin, RBC‟s, WBC‟s, platelets, MCV, MCH and haematocrit specifically. Statistical analysis was done by using SPSS...

Zubair Ali, Muhammad Sohail*, Yasir Ameen, Hamidullah, Ishtiaq Ahmed and Mehwish Malik

Jameel Ahmed1,2*, Mohammad Masood Tariq1, Nadeem Rashid1, Asim Faraz3*, 
Majed Rafeeq1, Irfan Shehzad Sheikh1, Mudassar Jahan1,2, Abdul Fatih1,2
Masroor Ahmad Bajwa1, Ifrah Jameel1, Muhammad Ali1 and Saira Iftikhar4
...ce in Pakistan. Randomly sampled animals of this breed were subjected to genetic analysis using ovine molecular markers where fifteen sheep SSR genetic markers were employed for describing multiple-allelomorphism. The average observed number of alleles (Na), effective number of alleles (Ne) and Shannon’s Information Index were 2.07 ± 0.79, 1.86 ± 0.69 and 0.54 ± 0.29, respectively. Heterozygosity was described by the average, observe...

Caiping Duan

...e-linked immunosorbent assay. Coronary blood samples were obtained during coronary angiography. Expressions of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF -α) were detected by Western blotting (WB). The protein contents of B-cell lymphoma factor 2 (Bcl-2), apoptosis-related genes Bax and caspase-3 were detected by WB. The contents of lactic dehydrogenase (LDH), creatine...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
...was the only methylation sample in IGF1 (2.23 ± 0.92%), significantly higher in methylation level than that of female reindeer and sika deer (0.01<P<0.05). Methylation levels of KGF in antler mesenchyme were highly significantly higher in male reindeer and sika deer (45.53 ± 3.87% and 42.20 ± 1.91%, respectively), compared to female reindeer (0.00 ± 0.00%, P<0.01). Methylation level of NGF in antler mesenchyme was highly si...

Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3

...on. Immunofluorescence assay was done to check subcellular localization of these nonstructural proteins. The CC50 value for A. indica seed extract for HepG2 cells was 650 μg/mL. The extract concentration 500 μg/mL was found to reduce gene and protein expression of NS3 and NS5A by 50 percent. Results of our study shows that phytochemicals of neem seeds have strong antiviral potential against HCV without having any significant cytotoxic effects.

...

Amber Khalid1*, Amjad Rashid Kayani1, Muhammad Sajid Nadeem1, Muhammad Mushtaq1, Mirza Azhar Beg1 and Surrya Khanam2

Chenchen Liu1,2, Tong Wang1, Muhammad Khan3*, Xiaolin Cui2 and Yongming Li1,2*

..., Edu, and colonogenic assays. The molecular mechanism associated with induction of apoptosis includes ROS generation, G2/M phase arrest and inhibition of aerobic glycolysis. Inhibition of glycolysis was found to be linked with down-regulation of lactate dehydrogenase A (LDHA) and glucose transporter-1 (GLUT1). Finally, ALT effectively suppressed growth and induced apoptosis in cisplatin resistant A2780/CR cells. Taken together, our findings suggest that ALT c...
Ayesha Ahsan1, Rabia Arif2*, Samina Nazir1, Muhammad Saleem2 and Memunna G. Shahid1
...uences of Neurospora crassa and Sordaria macrospora by using different PTMs predictor servers. Phosphorylation and glycosylation in different species of Sordaria as well as N. crassa was calculated on Serine (S), Tyrosine (Y) and Threonine (T) residues by NetPhos and YinOYang.

...

Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4

...(control) that offered basal diet, while, T1, T2, T3 and T4 groups were supplemented 0.5, 1, 1.5 and 2% P. pinnata leaf powder (PPLP) in basal diet respectively. The experimental groups were observed for the performance parameters, antibody titer against Newcastle disease virus (NDV), relative weight of lymphoid organs (bursa, spleen and thymus) and enumeration of gut microflora. Results s...

Jianli Xiong1,2,*, Hongtao Ren2, Guanglu Li2, Xiaochan Gao2, Yong Huang2 and Shiyang Gao2

...licate. Geese received basal diets, supplemented with either 0 (control), 10, 20, 40, or 80 IU/kg VE. All geese were treated for 12 weeks. The results showed that VE supplementation had no significant effect on body weight, but significantly decreased the average daily feed intake and the feed/gain ratio. Moreover, compared with control, the thymus index, serum SOD and GSH-Px increased significantly, while MDA decreased significantly in the 20, 40, and 80 IU/k...
Syeda Fakhra Waheed1, Asim Aslam1*, Muti-ur-Rehman Khan1, Kamran Ashraf2 and Beenish Zahid3
...strains of infectious bursal disease virus (IBDV) has resulted in substantial economic losses in Pakistan despite mass and intense vaccination regimens. Infectious bursal disease (IBD) is one of the most important immunosuppressive diseases of the poultry and has been a constraint on sustainable food security around the globe, including Pakistan. This disease damages the bursa of Fabricius...

Gulzhan Kalenovna Mussayeva1*, Alikhan Meldebekovich Meldebekov2, Nurgul Alikhanovna Meldebekova2, Gulshat Imanzhankyzy Shaykamal1, Batyrkhan Azimkhanovich Buralkhiyev2, Nurkuisa Mirzabekovich Rametov3 and Kanat Zhumanov4

...olstein breed it is necessary to adhere to the standard of the breed in all genetic, constitutional, exterior and productive parameters. To fully realize the genetic potential of the breed it is necessary: to select for artificial insemination bulls from the line whose offspring are most productive and physiologically more adapted to the climatic, technological and forage conditions of the farm. When selecting, special atten...

Ovirup Bhushan Paul1, Shodipta Sharma Urmi2, Md. Ashraf Ali Biswas3* 

Lei Jiang, Yi Huang, Runfeng Yang, Xiaohui Jiang* and Hao Yan

... to investigate whether Hsa_circ_0009910 regulates cisplatin sensitivity of ovarian cancer cells by regulating the expression of miR-455-5p /PAX2. The expressions of circ_0009910, miR-455-5p and PAX2 mRNA in HOSEPiCs and ovarian cancer cells SKOV3 and SKOV3/DDP were detected by qRT-PCR. SKOV3/DDP cells were grouped according to the transfection of different genes. The sensitivity of SKOV3/DDP cells to cisplatin was detected by CCK8 ass...

Yang Hu, Jingxin Ding, Beilei Xu, Xiangming Sun, Guoyu Li, Hui Song, Zheng Zong and Wenlan Li*

...um fingerprints of blood samples collected at 10 different times were established by UPLC-Q-TOF-MS analysis. The estrogen-like activity of TGs was evaluated by determining the proliferation rate of Michigan Cancer Foundation-7 cells. The spectrum-effect relationship was analyzed by serum fingerprint of TGs to characterize the activity in vivo and identify the active compounds of TGs. In the results, a total of 75 chemical compounds of TGs were identified in se...

Muhammad Shuaib1*, Abdul Hafeez1, Ziaul Islam2, Assar Ali Shah3 and Saad Ullah1

...orn-soybean meal diet (Basal Diet) was offered to the control group (A), while groups B, C, and D were fed the basal diet with 3, 6, and 9 % soybean hulls (SH) respectively. Results showed higher(P<0.05)crude fiber, ash, cellulose, and hemicellulose content for diets containing soybean hull (SH) at 3, 6, and 9% compared to the basal diet. Egg weight and egg quality parameters during dif...

Ghulam Mustafa Solangi4, Zaheer Ahmed Nizamani1*, Mansoor Tariq1, Zubair Ahmed Leghari2, Asghar Ali Kamboh3, Barirah Rehman Talpur1 

...ce, a total of 800 serum samples from four districts of Sindh (Thatta, Tharparkar, Jamshoro and Larkana) representing four agro-ecological zones of Sindh were examined by using c-ELISA. The overall seroprevalence of CCPP in goat population was found to be 18.0%. The prevalence of CCPP varied significantly (p<0.001) in the four districts. It was found highest in Tharparkar (24.5%) follow...

Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi 

...After diluting the semen samples were stored at 5oC for 4 hours. The observed variables included spermatozoa motility, spermatozoa survival, spermatozoa abnormalities and intact plasma membranes of spermatozoa. The obtained data was analyzed using the Analysis of Variance (ANOVA). The results showed that the addition of tomato juice extract into egg yolk citrate diluent exhibited a 20% increase in sperm motility percentage, 26.3% in sperm survival rate and 22....

Doaa H. Assar1, Rasha A. Al-Wakeel2, Zizy I. Elbialy3, Mahmoud M. El-Maghraby4, Helmy K. Zaghlool5, Adel A. El-Badawy4, Abdel-Khalek E. Abdel-Khalek6*

... (controls) were fed a basal diet without treatment. In G2 and G3, the daily diet was supplied with 1 and 5 g SPL /kg from 10 to 15 months of age. Dietary SPL supplementation significantly increased body weight, count of red and white blood cells, packed cell volume, and serum total protein, high-density lipoproteins, testosterone, and thyroid hormones (T3 and T4), and significantly decreased activity of aspartate and alanine transa

Yusmarini Yusuf*, Usman Pato*, Shanti Fitriani, Emma Riftyan, Evy Rossi, Diky Arma Fauzi, Ghina Ismadiah, Miftahul Hidayah, Windy Sabiliani

... nitrite (P2), 2.5% chitosan (P3), and 0.6% bacteriocin (P4). All samples were stored at freezing temperatures for 30 days. Data were analyzed statistically using analysis of variance, and differences between treatments were analyzed using DNMRT at the 5% level. The results showed that the application of various preservatives might impact the quality of fishballs during the first 30 days of frozen storage. Even though the ch...

Theresia Nur Indah Koni*, Tri Anggarini Yuniwati Foenay, Stormy Vertygo

...hydrates such as palmyra sap and nitrogen from Non-Protein Nitrogen such as urea. This experiment aimed to evaluate the fermentation characteristics and nutrient content of rice bran fermented anaerobically for six days. This experiment used a Completely Randomized Design with four treatments and four replications. The treatments included rice bran without palmyra sap and urea (T0), rice bran with 2% urea (T1), rice bran wit...

Doungnapa Promket1,2,4*, Khanitta Pengmeesri2,4, Jennarong Kammongkun3, Thassawan Somchan2

...was extracted from blood samples for genotyping using specific primers and restriction enzymes for each gene, and polymerase chain reaction-restriction fragment length polymorphism was used to identify the genotypes (PCR-RFLP). Three genotypes were found for each gene as BB, Bb and bb for NPY; TT, TC and CC for DRD2 and II, ID and DD for VIP. Genotype frequencies of NPY (range 0.13-0.58), DRD2 (range 0.06-0.55) and VIP (range 0.14-0.57) were reported. For the ...

Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*

...rds. Pseudomonas aeruginosa (P. aeruginosa) is opportunistic pathogens involved in veterinary disorders including clinical mastitis in buffaloes. This study aimed to investigate the antibiogram pattern and synergistic effect of amikacin and norfloxacin against resistant P. aeruginosa isolates from mastitis origin. In addition, detection of some virulence and antibiotics resistance genes. T...

Zulaikha Zainool Abidin1, Fahim Hafiy Idris2, Suriya Kumari Ramiah1, Elmutaz Atta Awad1, Zunita Zakaria3,4, Zulkifli Idrus1,2* 

...mportant to maintain the safety of broiler chicken meat.

...

Fathin Faahimaah Abdul Hamid1, Mohd Farhan Hanif Reduan1*, Jasni Sabri1 , Faez Firdaus Jesse Abdullah2,Mohammed Naji Odhah1, Nur Athirah Binti Abdul Manaf1, Mohd Jefri Norsidin2 , Siti Nor Che Yahya1, Intan Noor Aina Kamaruzaman1, Nur Zul Izzati Mohd Rajdi1 

...on was inoculated intranasally to all goats except goats of Group 1 which served as the negative control, Group 2 was the positive control, Group 3 goats treated with oxytetracycline, Group 4 goats were treated with flunixin meglumine, and group 5 received both oxytetracycline and flunixin meglumine treatments. Blood samples were collected at 24 hours, days 5, 7, 9, 11, 14, 21, and 28 post-inoculation for the clinical pathol...

Omnia M. Khattab1, Morcos I. Yanni2, Hala K. Abdelmegeed2, Mahmoud Eliwa1, Naglaa M. Hagag1, Sara M. Elnomrosy1

... study Fifty-seven fecal samples were collected from suspected cases of cats. 19 fecal samples were positive using Rapid FLV Ag test kits kit (VDRG FLV/FIV Ab rapid kit). Fifty-seven blood samples of the same animals were subjected for hematological examination to investigate the severity of leukopenia. Thirteen out of nineteen s...

Safaa Rhaimi1, Sara Brikat2, Mouloud Lamtai2*, Mohammed Ouhssine1

...te the acute toxicity of salvia officinalis essential oil in female Wistar rats, and to explore the antidepressant-like and anxiolytic-like effects and memory enhancing of the safe dose of this essential oil. In this experiment, salvia officinalis essential oil was administered once by oral gavage to four groups of female Wistar rats (n=6) at three diffe...

Siti Fairus Mohamed Yusoff1, Annie Christianus1*, Yuzine Esa1, Muhammad Fadhil Syukri Ismail1, Bashiru Garba2, Nik Siti Zaimah Safiin3, Nur Hamid Hidayahanum4

...he crude lipid and total saturated fatty acids (SFAs) were significantly higher in hybrid PH×PN and P. hypophthalmus. Also, crude protein and docosahexaenoic acid (22:6n-3, DHA) were higher in the hybrid PH×PN and P. nasutus. However, there were no significant differences recorded between the hybrid and its parents for omega-3 (n-3PUFA), total polyunsaturated fatty acids (PUFAs...

Riswandi1*, Ali Aim1, Muhakka1, Afnur Imsya1, Agus Wijaya2

...tochemicals (tannins and saponins), including water mimosa, giant molests, and water chestnuts. The objective of this study was to evaluate the effect of swamp forages combination in the ration on the characteristics of rumen fermentability and methane production. The design used was completely randomized design (CRD) with 4 treatments and 4 replications. The treatments given were A (guinea grass and concentrate, 7:3), B (gu...
Heavy metals (HMs) are harmful and lethal at negligible levels and non-biodegradable in the typical ecosystem and constitutes animal, human and environmental hazards. They are divided into toxic metals like Lead, Cadmium, Arsenic, etc. and essential elements like copper, zinc, manganese, iron, nickel and chromium. Additionally, could be categorized into two groups based on the natural and anthropogenic sources releasing origins. Population and industrial expansion led to food contamination with HMs. Poisonous metals can be transferred from irrigation water to agricultural soils, agricultural operations, air pollution, animal feed, and packaging materials. Toxic metals are non-biodegradable, non-thermos degradable, and exceedingly stable in the ecosystem; as a result, they quickly build in various foods. Metal pollution of many foods, including agricultural commodities, and animal protein sources such as fish, milk, meat, and eggs, poses a hazard to food safety and security. Toxic metal pollution of irrigation water, agricultural soils, plants, and animals result in their integration into the food chain, posing a health hazard to humans. Most metals are harmful to animals and humans and accumulate in several organs like the skeleton, hepatic tissue, spleen, and renal tissues. Metals have a deleterious impact on the production of plants and animals. As a result, several remediation strategies have become necessary to limit the hazardous HMs pathway into the food chain and the human body. Metal nanoparticles are employed in beneficial applications, although they are associated with specific hazards.
 
Keywords | Food contamination, Heavy metals, Nanoparticles, Pollution sources, Remedy, Soil contamination
..., poses a hazard to food safety and security. Toxic metal pollution of irrigation water, agricultural soils, plants, and animals result in their integration into the food chain, posing a health hazard to humans. Most metals are harmful to animals and humans and accumulate in several organs like the skeleton, hepatic tissue, spleen, and renal tissues. Metals have a deleterious impact on the production of plants and animals. As a result, several remediation stra...

Imran Khan1*, Shahid Amin2, Safiullah3, Shah Zeb Khan3, Abidullah3, Amjad Ali3, Saghir Imdad Hassan3 and Israr Ud Din3

...ls. A total of 300 blood samples were used for diagnosis. After analyses, 5.67% (17/300) samples were found positive for Trypanosoma evansi using thick and thin blood smears techniques as per method described by (Adam et al. 1971). A higher infection rate was found in females (6.67%) as compared to males (4.67%). Highest (9.00%) infection was noted in camels above 4 years of age group followed by (5.00%) in camel under 2 yea...

Anhar Ibrahim Elhanafy*, Amr Mohamed Mousa, Amaal Mohamed Kamal 

...lipoprotein, and vice versa regarding the high-density lipoprotein concentration was observed, compared to control. Moreover, BV caused a decrement in malondialdehyde and nitric oxide levels whilst it enhanced GPx activity, SOD, IgG and IgM, compared to control. Furthermore, BV treated does have significantly higher levels of C3 and C4. Conversely when compared to control, significant reduction in IL-1β, IL-6 and TNF-α was recorded. In addition, BV ...

Hussein Jabar Jasim1*, Amer Murhum Al-Amery2

...of ginger extract at a dosage of 5mg/ml with 24, 48, and 72 hr. treatment times was 90.1, 95.8, and 99.2% respectively, compared to the positive and negative control groups, which had ovacidal effects of 83.1 and 16.88%, respectively. But, the ovicidal efficacy of ginger methanolic extract at a dosage of 10 mg/ml is 97.4, 100, and 100% respectively, with treatment times of 24, 48, and 72 hr. While, using ginger extract at do...

Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi

...d humanely; ileum tissue samples were collected before and processed for histopathological evaluation. Improvement of tissue and the number of oocysts on the villi decreased after treatment. In comparison with G2, oocyst shedding was stopped at the end of the treatment period in both G3 and G4 without recurrence 10 days after drug withdrawal. The pre-patient periods (p.p) was 8-9 DPI. The maximum intensity was 11,000 oocysts per gram of feces. The first clinic...

Oussama Zghari*, Mouloud Lamtai, Sofia Azirar, Mohamed Yassine El-Brouzi, Hajar Benmhammed, Aboubaker El-Hessni, Ali Ouichou, Abdelhalem Mesfioui

...nsidered as an antidepressant, anxiolytic substance and protects neurons from OS. The present study was designed to evaluate the neuroprotection effect of MEL against Al neurotoxicity and OS in male Wistar rats. Rats received an intraperitoneal and/or a single intrahippocampal injection of NaCl, MEL or AlCl3. Before two weeks of intrahippocampal surgery period, MEL (4 mg/kg) was intraperitoneally injected (Group II and Group IV). Thereafter, control group (gro...

Soedarmanto Indarjulianto1*, Alfarisa Nururrozi1, Yanuartono1, Sitarina Widyarini2, Dhasia Ramandani3 

...strils, swollen on the nasal bridge and maxillary sinus. Subsequently, the cat was diagnosed infected by cryptococcosis based on cytology, fungal culture, serology, and hematology. Lymphadenopathy and the spreading of infection to the lungs were identified based on histopathological examination. Owing to potential zoonotic, a high index of suspicion was needed to identify mimickers like Cryptococcus spp. which in this case had initially presented as bilateral ...

Imran Muhammad Siddique1, Naseem Sharif1*, Abdul Ghaffar2, Mudassar Naseer1, Ammara Noreen3, Naseem Akhtar4 and Safeer ud Din5

...s 11.70% remained in the same strain (LSKS-2). Vitamin C was18.43 mg/100gms besides total phenolic compounds 94.78 μgms in LSKS-2. However, somewhat tenacious fruit with more peel thickness was observed in LSKS-4 which was 3.31 mm. Highest firmness remained 2.48kg in LSKS-1 of less seeded kinnow. Conclusively less seeded strain LSKS-2 proved best of all the five strains of less seeded kinnow. The study is worthwhile for exploiting natural mutant kinnow stra...
Muhammad Arabi Awan1, Hafiz Husnain Nawaz2*, Naeem Akhtar2 and Azmat Ali Awan3
...insect that feeds on the sap of olive trees. It belongs to the family Psyllidae, which includes other plant-sucking insects. The psyllid can cause significant damage to the olive tree, affecting its growth and yield, and also reducing the quality of the olives produced. A study conducted in 2020 at the agricultural research center in Tarnab, which evaluated the effects of different pesticide treatments on the population of olive psyllids in an olive orchard. T...

Siti Azizah1*, Salsa I Latifah1, Irfan H Djunaidi1, Anif M Wati1, Achadiah Rachmawati1, Siti Hamidah2 

...scale. We obtained 50 peasant women involved in adegen beef cattle farming as respondents in this study through Quota Sampling. We analyze the data by descriptive qualitative analysis. The results show that wives are productive in households, including on-farm, off-farm, and non-farm sectors. The contribution of wives who perform productive roles is essential. It is at 29.06%, with an outpouring labor of as much as 78,128 man-days/year and a producti...

Ijaz Haider1,2*, Muhammad Riaz2, Sikandar Ali2, Qurban Ali1, Ali Noman3, Dilbar Hussain1, Imran Nadeem1, Muhammad Faheem Akhtar1, Aqsa Abbas1, Asad Aslam1, Hafiz Saad Bin Mustafa2, Ejaz Ul Hassan2, Muhammad Zubair2, Muhammad Saleem1 and Muhammad Kamil Malik1

Muhammad Ehsan Safdar1, Muhammad Asif1, Amjed Ali1, Ahsan Aziz1, Naeem Akhtar2, Muhammad Shahid Gulrez1 and Waqas Raza3*

... combinations as their basal application to soil as boric acid and ZnSO4 fertilizers, respectively. RCBD with three blocks was applied. Seed of AARI Canola variety was sown in 45 cm apart rows. Maximum CGR (155 g m-2day-1), total dry matter (10663 kg ha-1), LAD (360 days), LAI (4.6), and NAR (34.4 g m-2 day-1), plant height (195.0 cm), and silique length (6.06 cm) biological yield (15.67 tons ha-1), seed yield (3.85 t ha-1) and oil yield (1188.3 kg ha-1) of ca...

Mostafa Mohamed Attia Hammam, Moawad Mohamed Mohamed Mohamed* and Mahfouz Mohamed Mostafa Abd-Elgawad

...ological nematicides are safe and promising alternatives. The present study examines M. arenaria damage on faba bean and presents effective control methods as Egypt is the world’s largest importer of faba bean. Using gain thresholds, the study compares costs of a biological versus a chemical nematicide for M. arenaria control on faba bean. Meloidogyne arenaria was not detected at pre-planting soil samples of two consec...

Usama Saleem1, Muhammad Asrar1*, Dilbar Hussain2, Saddam Hussain1, Abdul Ghaffar2, Hassan Usman1, Muhammad Imran1, Mubshar Saleem3 and Maryam Hamid1

...t damage by sucking cell sap, injecting a poison into the plant, eliminating photo-assimilates, and spreading plant-destroying viruses. This study aimed to investigate the effectiveness of botanical extracts and synthetic insecticides in combating wheat aphids. Data regarding the aphid population was recorded 24, 48, 72, and 168 hours after the application of plant extracts and insecticides. Maximum mortality recorded after 168 hours was, 85.06% and 79.29% by ...

Muhammad Ahsan Riaz1, Ayesha Riaz2, Muhammad Asif Mahmood3, Muhammad Uzair Mukhtar3*, Zaib Un Nisa1, Beenish Ijaz4 and Muhammad Shahid Rasool5

...nt College University Faisalabad for further analysis. The larval morphological measurements were carried out using a stereomicroscope, which included changes in head length and width, thoracic length and width, abdominal length and width, and total length of the larva. Every month, the fourth instar larvae (n=36) were investigated for body size measurement. The results showed that low temperatures of breeding water significantly increase (P≤0.05) the body ...

Ahmad Jupri1*, Yuliana Vofi2, Faturrahman2, Immy Suci Rohyani1, Ernawati1, Bulkaini3, Djoko Kisworo3, Wardatul Jannah4 

... of which is in Joben, Pesanggrahan Village, Montong Gading District, East Lombok Regency. Drinking water from springs can be polluted by contaminants such as bacteria, viruses and others during storage and distribution. This study aims to analyze the quality and microbiological feasibility of drinking water sourced from Joben Springs, Montong Gading, East Lombok. The research was carried out in May-June 2021. The research method used was purposive

sans-serif; font-size: 14px; background-color: rgb(255, 255, 255);">Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal and Gehn Allam

...between 16-36 years. All samples were tested for HPV-DNA by polymerase chain reaction (PCR) amplification using one pair of general primers which allowed for the amplification of HPV types 16, 18, 31, 33, 52, & 58. These primers were designed to amplify E6/E7 gene junction sequences. Twenty nine out of 100 (29%) samples of aborted products of conception were positive for HPV E6/E7 sequences. In comparison. only one of th...
M. A. Abou El- Nasr1, Kh. A. Dougdoug1, Hayam S. Abdelkader2, and Rehab A.
Dawoud2
... potyvirus (PPV), the causal agent of Sharka disease of Prunus inflicts severe crop losses in affected El-Amar area. The virus isolated from EL-Amar apricot trees, was propagated on apricot healthy seedlings. Degenerated oligonucleotide primers were designed to amplify the N-terminal portion of the capsid protein of PPV. The amplified products were cloned into pGEM-T-Easy vector and hybridized to PPV DNA specific probe labeled with Dig-I 1dUTP. DNA sequencing ...

M. A. Amer1; M. H. El-Hammady2; H. M. Mazyad1; A. A. Shalabyl and F. M. Abo-El-abbas

...using western blotting assay on nitrocellulose membrane using monoclonal antibodies verified that the PVY-CP gene correctly encoded and expressed a protein reacting with PVY antibodies. The CP gene, approximately 32 KDa reacted successfully with P VY specific monoclonal antibodies in dot blot immunobinding assay (DIBA) as well as in western blot assay. The nucleotide sequence of the Egypti...

Om-Hashem M. EI-Banna1, M. A. S El-Kady2, E. A Salama1 and Salwa N. Zein2

...D 18) of Barley Stripe Mosaic virus (BSMV) revealed a high degree of sequence similarity of γ RNAs comparing to ß RNAs of both strains. On the Other hand, nucleotide, and amino acid sequences of the two strains revealed differences in the 5' untranslated region (UTR) between the coat protein and ꞵb gene. It was clear that five bases, and 18 out of 1 16 amino acids were found different. three amino acids insertion, and one amino acid deletion. Lit...

A.Y. Ahmad1, M. A. Amer2, T. A. Mostafa1, F. M. Abo El-Abbas1, and M. El-Hammady1

E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2

...(Vitis vinifera L.) leaf samples obtained from El Kalubia Governorate. Three out or 25 samples were positive for GFLV by DAS-ELISA using specific polyclonal antibodies raised against purified GFLV preparation and five samples were positive by RT-PCR. RT-PCR detected GFLV in both fresh and dried tissues. A fragment (321...

Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2

...mic necrosis, systemic mosaic yellowing, bronzing, or stunting. The virus transmitted by mechanical means as well as by Thrips tabaci. Its TIP was between 45-50oC, DEP was 10-5 and LIV was between 5-6 hrs. Local lesion technique for biological purification and electron microscopic analysis confirmed the production of mature infectious virus particles underlining the conclusion that a full infection cycle was completed in this system. Both the structural viral ...

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...hain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd cDNA probe.

...

Om-Hashem M. El Banaa l, A.A. Abou Zeid2, Fawzia I. Moursy3 and Azza, G. Farag

...ri Saglio et al), the causal organism of citrus stubborn disease. RecA gene protein is the enzyme primarily responsible for the homologous recombination of chromosomal DNA in bacteria. The Immunocapture- Polymerase Chain Reaction (IC-PCR) technique was applied for detection of S. citri Saglio et al in which the Spiroplasma was captured with the specific polyclonal antibodies on a solid-phase. Specific primers based on the sequence of the Morocco strains of S. ...

Sahar. A. Shoman1 and B. A. Othman2

...e detection or Tobacco mosaic virus- Egyptian strain (TMV-E). Total RNA extract of infected plant and RNA of purified TMV particles were subjected in this method with four degenerate and undegenerate primers. The primer pairs generated two specific PCR fragments of 3428 base pair (bp) and 3836 bp of the whole TMV genome. The intensity of these RT-PCR amplified products was estimated, and it indicated relatively to the amount of the virus in the infected plant,...

M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1

...olate Of Barley stripe mosaic Hordevirus (BSMV). The 1st procedure was successful in producing relatively high yield of virus preparation 3.7 mg/100 g of barley leaves with adequate purity and one light-scattering zone was round in sucrose density-gradient column. The 2nd and 3rd procedures yielded aggregated virions (2.3 and 7.0 mg/ 100 g of fresh barley leaves, respectively). Electron microscopy of negatively stained purified virus preparation showed rod-sha...

A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1

.... Cara. Nicotiana glutinosa L. Nicotiana benthamiana gave systemic infection only. On the other hand. systemic symptomless infection had occurred with N. tabacum L. cvs. White Burley and Xanthine. Whereas PAMV didn't infect Ch. umaranticolor Cost. Reyn and D. metel L. The virus was transmitted by aphids (M persicae) in a non-persistent manner only when the aphids fed firstly on PVY N source for 10 min. This indicated a kind of synergistic effect between the tw...

S.A. El-Kewey1; R.A. Omar1; S.A. Sidaros2 and Samaa Abd El-Khalik3

...pe preparations of crude sap extracted from naturally infected garlic plants and negatively stained with 2% PTA showed flexuous rod-shaped particles 657-714 nm long. Biological relationships between the virus and insect vectors were studied.

...

 Salwa N. Zeinl and Hanaa S. Zawam2

... dot immunobinding assay (DIBA). The presence of the virus was checked in blossom parts. (Petioles, stigma, and flower cup).

...

Waheed Khan1, Muhammad Ajmal Khan2, Bakhtawar Khan1*, Aftab Amin3, Muhsin Ali4, Awais Farid, Ali Hazrat5 and Muhammad Yahya5

...and after 28 days, blood samples were collected to measure the lipid profile. The result showed that methanolic extract of V. thapsus significantly (P>0.05) reduced the blood glucose level, TC, TG, LDL, increase HDL and body weight at 400mg kg-1 compared to 200 mg and 10 mg of the standard drug after 28 days of treatment. The results of our study suggested that methanolic extract of V. thapsus have potent anti-diabetic activity, with comparatively less toxi...

Asim Faraz1, Amal AlKharusi2, Nasir Ali Tauqir3, Muhammad Shahid Nabeel4, Syeda Maryam Hussain5, Abdul Waheed1*, Muhammad Arslan Akbar6

Muhammad Ismail1, Abu Bakar Muhammad Raza1,2, Zarina Qasim3, Muhammad Zeeshan Majeed1*

...of fruits. Bactrocera dorsalis Hendel is an emerging threat to the export of Pakistani citrus fruits to overseas markets. Integrated pest management (IPM) is an appropriate and sustainable strategy for controlling fruit flies as it is based on integrated application of all available pest control methods with minimum implication of hazardous synthetic insecticides. In this study, five treatments including field sanitation (T1...

Tanvir Ahmed1, Sachchidananda Das Chowdhury1, Bibek Chandra Roy1Shubash Chandra Das1*, Khan Md. Shaiful Islam2, Bipul Chandra Ray1, Sukumar Saha3 

...biotic (PB), mannan-oligosaccharides (MOS) enriched with β-glucans, and an antibiotic growth promoter (AGP) on growth performance, intestinal microbial load, and immune response in broiler chickens. A total of 450 Cobb-500 unsexed DOCs were divided in response to the treatment into broilers fed a control diet (no additives), control supplemented with PB (100 g/100 kg feed), or control supplemented with AGP (Lincomycin 2.2%, 15 g/100 kg feed), having six r...

Shabana Mangi1*, Abdul Manan Shaikh1, Waheed Ali Panhwar1, Javeed Ali Ujjan1, Fakhra Somroo1, Shazia Praveen Solangi2, Sumbul Mureed Mastoi3 and Ranjeet Kumar1

Nguyen Thi Kim Dong1*, Nguyen Van Thu2 

...yme (VE)to concentrate-basal diets corresponding to the S0, SP0.2, SP0.4, VE0.4 and VE0.8 treatments. The results indicate that the treatments supplemented SP and VE improved final live weight and daily weight gain (p<0.05) and the birds in the PS0.2 treatment had the highest daily weight gain (17.3 g/day), final live weight (1543 g/bird) and better feed conversion ratio (p<0.05). Similarly, the carcass, breast and thigh weights were significantly higher...

Muhammad Ather Nadeem1, Bilal Ahmad Khan1,2*, Neelam Yaqoob3*, Abdullah2, Amina Iftikhar4, Muhammad Irfan5, Hafiza Zara Saeed6, Syed Saqlain Hussain7 and Sidra Shakil8

Riaz Hussain2*, Adnan Ihsan1*, Waqar Khan1, Shiraz Malik1, Hamza Jameel1, Hidayat Ullah3, Shahab Ahmad4, Jawad Anwar5 and Murad Ali6

... Garlic Fresh Cloves (A. sativum), Chinaberry Fresh leaves (M. azedarach), Eucalpytus leaves against pea aphid Acyrthosiphon pisum. The research experiment was design randomize complete block (RCBD) with three replications and two sprays were applied at 14 days interval. After 7 and 14 days of 1st and 2nd application the minimum means density of aphids were recorded in plot treated with Eucalpytus leaves (8.33, 4.66) and (5, 3) respectively and followed by Gar...

Behari Lal Meghwar1*, Attaullah Khan1, Imran Ali Lakhiar1, Asif Ali Mirani2, Muhammad Siddique Daper1 and Muhammad Waseem Kalroo

...In addition, the chilies samples were collected (uniform in color and size) from the farmer’s field and brought to the experimental site. The experiment selected chili variety was Longi, and the initial weight of the all collected samples was 2000 g. The selected samples’ initial moisture content was considered to be 80%. The test results showed that T1 and T2 methods significa...

Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5

...text-align: justify;">Physalis minima is a promising medicinal plant of the family Solanaceae, but its survival is under threat, and limited long-term conservation approaches are available. We employed an in vitro plant tissue culture technique to develop a mass propagation system for the effective multiplication and conservation of P. minima. Juvenile leaves were cultured on MS basal medium supplemented with 0.1, 0.5, 1.0, ...

Yun Zhang*

...aged 28 to 40. The blood samples were collected, and exosomes were isolated using commercial kit. The exosomes were observed by transmission electron microscope. Western blotting was done to detect the protein expression. Transmission electron microscope analysis revealed that exosomes could be significantly observed in the PE group. The expression of surface markers that included CD63 and CD81 were dramatically upregulated in PE group. The protein level of ET...

Xiaoying Liang1,2, Xiaofang Jiang3, Honglin Jia1, Ru Zhang1, Li Gao4* and Qing Yang5*

...by effector T cell polarisation. This study investigates changes in the marker genes of T cell subsets after allergen sensitisation and demethylation intervention as well as the T cells’ effects on allergic airway inflammation development. The number of T cell subsets and the expression of cytokines were detected by a well-established model of experimental asthma. The number of T cell subsets was detected by flow cytom...

Fang Chen

... at 37°C. The cell assay was performed when the cells were in the logarithmic growth phase. In the cultured H69/apatinib, the IC50 of apatinib was greater than 30nM, while it was about 13μM in H69 parental cells (P<0.05), indicating that the anti-apatinib SCLC cells were successfully constructed in vitro. The expression of miR-4728-3p mRNA in the miR-4728-3p inhibitor group decreased, and the expression of PTEN mRNA increased (P<0.05). The miR-472...

Yisi Chen*, Jun Zhang and Fayin Li

...;0.05). The number of dorsal spinal cord neurons co-expressed with EPAC1 and c-fos was significantly decreased in the propofol group after surgery (P<0.05). To conclude, propofol could reduce postoperative pain response in animals and inhibit the glun2b-p38mapk /EPAC1 signaling pathway in the spinal cord.

...
Saiwa N. Zein
...ceae. Ebenaceae. Leguminosae and Solanaceae. The identity of the virus to TRV was confirmed by mode of transmission. particle morphology and serological typing. Examination or the purified virus preparation 1» electron microscopy revealed rod-purify the Egyptian isolate of TRV. The yield of the virus was 2.6-5.7 mg/ 100 g tissue of Nicotiona rustica plants. The polyclonal antibodies raised against the Egyptian isolate of TRV had a specific titer of I: 10...
A.l. Abd El-Fattah; A.s. Saclik M.M. El-Kholi; I.A. Åbdcl-Hmnid and M.A. Madkour
...height:108%">Sugarcane mosaic pot.1Virus (SCMV) is the causal agent Of mosaic disease which is reported to be one of the most important viral diseases attacking sugarcane crop in Egypt. Therefore. the present work was designed to screen the SCMV strains in Egypt based on biological. serological and molecular characterization. Data of the use of Sorghum bicolor vars. Rio and Atlas show succ...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

...608 quarter’s milk samples were aseptically collected form 902 Friesian cows, of which 68 (7.54%) suffered from clinical mastitis and 120 (13.3%) proved to suffer from subclinical form of mastitis. Bacteriological examination of the milk samples revealed the isolation of the following pathogens from clinical and subclinical mastitis cases, respectively; E. coli (29.41% and 29.2%), S. aureus (19.12% and 25%), Str. agala...

Saad Zaghlou El-Damrawy1, Reda Ali Hassan2*, Ahmed Maher Bakhaty1, Adel M. Nasr2, El-Sayed A. Abu El-Hassan2  

... control (NC) with the basal diet, (2) NC + 600 mg/kg diet of silymarin, (3) NC + 400 mg/kg diet of choline, (4) Positive control (PC) containing 1 mg AFB1/kg diet, (5) PC + 600 mg/kg diet of silymarin and (6) PC + 400 mg/kg diet of choline. The findings show that the AFB1-treated chicks’ growth characteristics dramatically decreased. While total antioxidant capacity (TAC) dropped as a result of AFB1, serum levels of alanine aminotransferase (ALT), aspar...

Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz 

...ens and may pave way for safe poultry rearing and addressing public health concerns associated with AGP.
 

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Wuritunashun*, Burenbatu, Narenqiqige, Jiuguniang, Eerdunduleng, Shuanglian Wang, Cuiqin Gong, Hashengaowa, Huizhi Jin, Baiwurihan and Chunhaizi

... proteoglycan 4, lipopolysaccharide-binding protein (LBP), thrombospondin-4, and plasminogen activator inhibitor 1 were downregulated. Bioinformatics analysis of these differentially expressed proteins revealed that they play a critical role in immune-related responses. Parallel reaction monitoring validation also substantiated the results of the LFQ proteomics approach by analyzing the expression of LBP and SERPINA10. This work identifies the proteins and pro...
Muhammad Rizwan1*, Bilal Atta1,  Muhammad Arshad2, Sohail Akhter3
Muhammad Tahir3, Misbah Rizwan1, Muddassir Ali1 and Arshad Makhdoon Sabir1
...l crops since long. An assay was designed to evaluate the efficacy of λ cyhalothrin on biological parameters of generalist predator, Coccinella septempunctata. Results indicated that λ cyhalothin has no significant impact on developmental life stages of larvae of ladybird beetle but it significantly reduced the span of total adult longevity (30.8 to 26.91 d), female adult longevity (31.5 to 27.79 d), and male adult longevity (29.4 to 25.56 d) as ...

Tarique Ahmed Khokhar1*, Atta Hussain Shah1, Muneer Ahmed Jamali1 and Asghar Ali Kamboh2

... chevon and chicken meat samples. Nutritive values of chilled, frozen and thawed buffen sample also decreased with increasing storage period.

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Mansoor Ayoob1, Atta Hussain Shah1, Zaheer Ahmed Nizamani2, Muhammad Faisal Ayoob2,3*, Deepesh Kumar Bhuptani1,5 and Abdul Sattar Baloch4
... longissimus dorsi). The samples were analyzed for moisture, fat, protein, glycogen, peroxide, thiobarbituric acid, total viable count and total coliform count after marination with 0, 10, 20, 30 and 100% concentrations of honey and after 1, 4, 8 and 12 days of storage (4 ̊C). The moisture, fat and protein contents were moderately decreased on the 4, 8 and 12th days of storage in all the groups. The glycogen content showed a significant (P < 0.05) increase...

Keatisak Soisuwan1, Hatairat Plaimast2, Atichat Thongnum3, Soranot Chotnipat4, Manatsanun Nopparatmaitree4* 

...ed design (CRD). One thousand Ross 308® one-day-old chicks were divided into two treatments, each with 10 replicates and 50 chicks (25 females and 25 males) per experimental unit. Dietary treatment consisted of commercial broilers diets (corn-soybean based diet) supplemented with 0 or 100 g/ton of natural multi-enzymes feed supplementation (top-up feeding). Birds were provided ad libitum access to water and experimental diets. The results showed that natur...

Maria Endo Mahata1*, Oriyanti Br Situngkir1, Yan Heryandi2, Takayuki Ohnuma3, Yose Rizal1 

...effect of the type and dosage of pellet binder Miana plant (Plectranthus scutellarioides [L]R.Br) on crude fiber digestibility, nitrogen retention, and energy metabolism in broiler chicken. The study used 30 broiler male chickens with an average body weight of 1.5 kg at five weeks of age. This study used an experimental method with a completely randomized design (CRD) of a 3x3 factorial pattern which was repeated three times. Factor A (type of pellet binder):...

Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1  

...ion of miRNAs were assayed. Results: Findings indicated that CSO treatment led to a suppression in hepatotoxicity, nephrotoxicity and cardiotoxicity induced by DOX in male albino rats evidenced by a significant enhancement in the activity of ALT, GGT, LDH, AST, also creatinine and uric acid. CSO induced antioxidant activity GSH, CAT and inhibited MDA. Histopathological examination in sections of liver, kidney and heart tissues were confirmed these finding...

Adel B. Salama and Reham M. Sabry*

...ssibly improve the compensation ability of plants to set new buds. Petal harvesting had positive significant effects on plant fresh and dry weights, seed yield but adversely affected flower fresh and dry weights as well as seed oil percent (%). Seed oil percent ranged from 11.76 to 11.94 in petal harvesting treatment compared to control that ranged between 14.01 to 14.38. Petal harvesting resulted in a significant reduction in oil percent by 19 % and 20% when ...

Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3

...total of 838 bovine sera samples were tested through Solid phase competitive enzyme-linked immunosorbent assay (SPC-ELISA), based on structural proteins at the National Veterinary Laboratory (NVL), Islamabad from 10 January 2021 to 09 January 2022. The overall bovine population was carrying maximum seroprevalence of serotype O (28.52%) followed by seroty...

Fatimah Mohammed Alsarawi1*, Azharia Abdelbagi Elbushra1,2 and Ishtiag Faroug Abdalla1,3

... gap increased by 24 thousand tons annually. The model results showed a statistically significant positive association between domestic consumption of poultry meat and GDP, and a negative significant statistically relationship with meat price index in the long run. Furthermore, the results depicted a positive significant relationship in the short run between domestic consumption of poultry meat and GDP. The study recommended improvement of poultry sector produ...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...enes in 160 chicken meat samples, which are randomly collected from different regions of Northern Punjab, Pakistan. Isolation of pathogen from meat samples were performed with the use of International Organization for standardization based microbiological techniques, while chain reaction technique (PCR) was used for detection and characterization of Stx-1 and Stx-2 genes. In total, 75 (46.8%) isolates were detected as E. col...

Muhammad Saad Waqas, Li Xia, Tian-Ci Yi*, Liang-Yu Sun, Rong Xiao and Dao-Chao Jin*

...ms, and demonstrated how sanitary behaviour can result from a combination of evolutionary history. Nest relocation findings suggest that larvae, nymphs and adults adjust relocation patterns based on conditions they faced.

...
Gilyan V. Fedotova1,2, Marina I. Slozhenkina1, Ivan F. Gorlov1,  Aizhan A. Kaidulina1, Alexander A. Mosolov1, Daria A. Mosolova1, Mohammadreza Poorghasemi3 and Alireza Seidavi3*
...housed and fed under the same conditions. The bulls used were all classed as Elite. The feeding rate was intense. Steers in all groups were raised in the feedlot throughout the year. Growth rate of the crossbred steers was greater than the purebred steers. This was reflected in higher yields of the most valuable parts of the carcasses, the rump and saddle. Simmental crosses were distinguished by the lower fat content of thei...

Kétomon Pierre Challaton1*, Coovi Guénolé Akouedegni1, Kadoéito Cyrille Boko2, Goué Géorcelin Alowanou1,3, Pascal Venant Houndonougbo4, Aboudou Habirou Kifouly1, Mawulé Sylvie Hounzangbé-Adoté1  

... Benin. Thus, feces were sampled from 572 and 497 goats in the rainy and dry seasons, respectively, in southern, central and northern Benin. The parasite inventory was performed using the Mini-FLOTAC technique for the quantitative study and the Baermann method for the qualitative research. The results showed an overall prevalence of gastrointestinal parasites of 96.82 %. Furthermore, goats were mainly infected with coccidia (92.24 %); strongyles (83.91 %), of ...

Rahma Boukarine*, Leila Hamdi and Kamel Khelili

...ioxidant effect of Eruca sativa on the hepatic and renal profile damaged by xylene. Seventy rats were divided into seven equal groups and treated by gavage for 30 days as follows: Control group (C), group (CO), positive control group (RE) was received 350 mg / Kg bw of Eruca sativa aqueous extract (ESAE), 2 toxic groups X1 and X2 were treated with xylene...
Muhammad Fiaz1, Muhammad Farooq Hussain Faisal1
Muhammad Imran Khan1*, Kashif Ishaq1, Tanveer Ahmad1 and Qazi Jamil Akbar2

Atena Azarniush1, Majid Moghbeli2*, Farshid Kafilzadeh1, Mohammad Kargar1 and Hooshang Jamali1

...was observed in positive sample. This research demonstrated the efficacy of using B. subtilis as an expression system for the production of IL-11 and it can be used as a candidate for the proteins without post-translational modification.

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Widya Pintaka Bayu Putra1, Herman W Raadsma2, Indriawati1, Slamet Diah Volkandari1 and Endang Tri Margawati1*
...e average of LS in sheep samples was 1.34±0.51 with h2 value of 0.04±0.06 (low). However, six Merino rams (43%) had positive of EBV value. Moreover, the data records of LS from each observed ram were sufficient for evaluation and signed by a moderate relative accuracy (RA) value ranged from 0.27 to 0.71. It can be concluded that selection of Merino rams can be performed based on LS using EBV. 

...

Xiaowei Huang1, Chao Ma1, He Lin1, Yuchen Wang1, Yan Xu1, Guangfu Lv2* and Zhe Lin1*

...s mechanism of antidepressant effect, healthy Institute of Cancer Research (ICR) male mice were divided into control, chronic unpredictable mild stress (CUMS), fluoxetine (3 mg/kg), OR800 (OR 800 mg/kg) and OR400 (OR 400 mg/kg) dose groups. After the last administration, the content of corticotropin-releasing hormone (CRH), adrenocorticotrophic hormone (ACTH) and corticosterone (CORT) in serum were detected. Hematoxylin and Eosin (H&E) staining was used to...

Yong Bin Zhao1, Hui Song1, Guo Gong Yi1*, Xi Lian Hao1, Bai Wang2, Zhi Wen Chen2

...tured to collect feather samples in a way that clarifies the attribution of feather samples. As some endangered birds cannot be captured, feather sampling is the only feasible method for isotopic research. However, whether it can be used for research on individuals remains to be clarified. In this study, the values of δ13C and δ15N of the rachises and barbules of 27 feathers fr...

Anwar ul Haq1, Maqsooda Parveen2, Shehzadi Saima3* and Khalid Masood Ahmad1*

...ext-align: justify;">Chrysanthemum morifolium L. cultivators in Pakistan have frequently detected that poor root cuttings of chrysanthemum caused poor anchorage of roots that results in shipping losses of these pot plants. Optimum concentration of appropriate auxins is used to promote effective rooting. Completely randomized design (CRD) is adopted and it consisted of five treatments including control and different concentra...

Jihan Abd Wahab, Nur Bahiah Mohamed Haris* and Jasmin Arif Shah

...stributed using a random sampling technique. A total of 362 extension agents from Peninsular Malaysia were participated in this research. Based on the result, all independent variables (PIME skills) indicated a positive correlation towards work performance. The monitoring and evaluating are the skills that significant towards work performance, and the evaluating skill became the highest independent variable that contributes to the work performance of extension...

Hassan Raza1, Muhammad Kashif Afzal2, Muhammad Luqman1*, Tahir Munir Butt3, Muhammad Yaseen1 and Muhammad Umer Mehmood1

...g demand for edible oil, save foreign exchange, and improve the farming community’s livelihoods. It is a matter of fact that training plays an important role in building the farmer’s capacities, raising awareness, and providing them with modern knowledge by enhancing their performance to achieve the ultimate goals. However, farmers struggle to manage the olive crop due to a lack of practical knowledge and training sessions on advanced production te...

Seyi Olalekan Olawuyi1*, Adedotun Oluwagbenga Anjorin2, Oluwagbenga Titus Alao3, Tosin Dolapo Olawuyi3, Rachael Ajibola Ayinla3 and Rasheed Ayodele Ayinla3

...ugh a multi-stage random sampling technique. Data were analyzed using frequency distribution and percentages, cross-tabulation, and food insecurity experience-based scale for the continuum categorization of farmers’ food security status. Ordinal logistic regression model was applied to estimate the effect of adoption of bio-security practices and other dynamics on the farmers’ levels of food security status. Findings revealed that crop farmers were...

Muhammad Kefayatullah* and Said Wahab

... 28 days of storage. The samples T1 (control), SA1 (1 % sodium alginate), SA2(2 % sodium alginate), MC1(1 % methyl cellulose), MC2(2 % methyl cellulose), P1(1 % pectin), P2(2 % pectin), BW1(1 % bees wax), BW2(2 % bees wax), CC1(1 % calcium chloride) and CC2 (2 % calcium chloride) were studied for TSS, pH, acidity, asco...

Muhammad Hasnain1, Qudsia Nazir2, Ifra Saleem2*, Syed Shahid Hussain Shah1, Muhammad Asif Ali1 and Noor ul Ain3

...plied (in control) at Faisalabad and Chiniot (FSD and CHT), respectively. EZU also showed improved results of Zn contents in grains as compare to the treatment where ZnSO4 and urea was applied separately. On the other hand, with application of EZU, agronomic parameters including straw and grains yield, and panicle length were also improved significantly (2.7, 18.7 and 16.4%, respectively), as compared to urea and ZnSO4 application separately. Therefore, it was...

Abid Hussain1*, Hassnain Shah1, Muhammad Nadeem Iqbal2 and Tariq Sultan3

... soil health are indispensable to obtain better crop productivity. Various research institutions in Pakistan have developed, and promoted several technologies for sustainable soil management and to improve crop productivity. These technologies include use of micronutrients for citrus fruits and their application for off-season vegetables, use of Biozote for wheat crop, and Pak Seeder technology for sowing wheat crop by zero-till method. Keeping this in view, e...

Asmaa A. M. Abdel-Ghany1, Ahmed N. El Taweel2, Yassmin Moatasim2, Nagwa S. Ata1, Amany Adel3, Ayman Hany El-Deeb4, Ahmed Kandeil2, Mohamed A. Ali2*, Hussein Ali Hussein4* 

...entified among collected samples. Two representative H5N8 isolates from duck and goose were subjected to full genome sequencing and genetic analysis. Although both of H5N8 viruses were closely related to clade 2.3.4.4b, they were genetically distinct and had genetically different constellation forms. This study highlights the need of active surveillance of AIVs in backyard production sector for assessing the epidemiology, evolution, and diversity of currently ...

Nitya Salsabila, Djalal Rosyidi, Agus Susilo* 

...smoke and spices such as salt, coconut sugar, garlic, galangal, coriander and tamarind. The method used was a laboratory experimental method using a completely randomized design (CRD) with 6 treatments and 4 repetitions, namely P0: without the addition of liquid smoke (control), P1: liquid smoke without sonication, P2: sonication liquid smoke for 5 minutes, P3: sonication liquid smoke for 10 minutes, P4: sonication liquid smoke for 15 minutes, and P5: sonicati...

Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy 

...) supplementation in a basal diet on the semen quality of the Kacang Buck. This study was conducted on 10 mature bucks with good libido and an average age and body weight of 2.7±0.35 years and 28.4±2.4 kg, respectively. All experimental bucks received a daily diet of native grass equal to 10% of body weight from day 1 to day 20. From days 21 to 35, they received a diet the same as days 1 to 20, in addition to S...

Natalia Shchur1,2*, Olha Chechet1, Tetiana Mazur2, Oleksandr Martyniuk2, Olga Gorbatiuk1, Halyna Buchkovska1, Iryna Musiets1, Diana Ordynska1, Olena Finkova3, Larisa Moskalenko3, Tetiana Ponomaryova-Gerasimyuk3, Maksym Lusta3, Vitalii Nedosekov2 

...on and research of 2,120 samples of cecal contents from cattle, pigs, and poultry. The samples were analyzed by a microbiological method for the detection of Campylobacter according to the scheme of accumulation, isolation, isolation and identification according to DSTU ISO 10272-1:2007. As a result, 33 isolates of Campylobacter spp. were isolated, which is 1.6% of the total number of the researched

Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi 

...es. A total of 100 fecal samples were collected from quails in various parts of Baghdad/ Iraq from January to September, and they were then tested for the presence of Cryptosporidium spp. using molecular methods (PCR), followed by DNA sequencing. The overall infection rate was 37% (37/100) of the samples were Cryptosporidium-positive by using PCR. Three of five quails whose 18S gene was amplified by nested PCR and sequenced ...

Sahar Ezeldien1,2, Foromo Dramou2, Fatma M. Yousseff3*, Alexander A. Nikishov2, Sergy B Seleznev2

...omile extract were all assayed. The results showed that the chamomile aqueous extract is rich in phenolic(108.92 μg/ml) and flavonoid (66.41 μmol/ml ) compounds. Additionally, chamomile extract is a potent antioxidant(1.77 ng/ml). Laying quails supplemented with chamomile aqueous extract showed increased body weight and egg mass, as well as a lowered feed conversion ratio. Chamomile extract significantly increased serum levels of total protein and albumi...

Ngoc Tan Nguyen1*, Tan Loi Le1, Thi Kim Ngan Vo1, Chi Hieu Do1, Tuan Thanh Hoang2, Nguyen Khang Duong3, Quang Minh Luu4

...n this study, with blood samples collected from the wing vein for DNA extraction to amplify the fragment length with 536 bp by PCR, and then the PCR products were cleaved with the PstI enzyme. The fragment length with 536 bp was successfully amplified in all individual samples, while genetic polymorphism at the PRL/PstI site was identified as C and T alleles, with allele frequencies 0.811 (C) and 0.189 (T). Three genotypes w...

Edwin Ormachea V1,2*, Bilo Calsin C1,2, Eyner Aguilar S3, Buenaventura Ormachea V4, Henry Gonzales C1, Yecenia M. Masias G5

...tal diameter; Sternal dorsal diameter; Rump width; Rump length; Height at the rump; Leg perimeter; Height at hock; Metacarpus perimeter and Metatarsus perimeter. Data were processed using R statistical software. The correlation of the main morphological characteristics were significant and high. Age significantly influences morphological characteristics, showing high correlations between characters. Two principal components were obtained for sheep morphologica...

Munasik*, Titin Widiyastuti, Caribu Hadi Prayitno

...y explores the use of cassava (tapioca by-products) and tofu dregs as substitutes for corn through fermentation technology using Neurospora sp. This produces alternative feed ingredients with nutrient levels that are in balance with corn. To improve the quality of the cassava-tofu dreg mixture, this study used red oncom mushrooms enriched with Na-glutamate during the fermentation process. The research used a completely rando...

Bambang Hartoyo1*, Tri Rachmanto Prihambodo1,2, Wahyuningsih3, Sri Rahayu1, Fransisca Maria Suhartati1, Muhamad Bata1, Efka Aris Rimbawanto1

Muhammad Awais Ahmad1, Mudssar Ali1*, Asif Sajjad2 and Shafqat Saeed1

...y A. mellifera and A. dorsata. The seed set in open pollination (free insect visits) was 58 and 70% higher and seed weight of 1000 seeds respectively as compared to self-pollination (no insect visits). Hence, conserving these most efficient native pollinators can lead to higher seed yield of T. alexandrinum and other seed crops in South Punjab, Pakistan.

...

Mahpara Fida Ahmed1, Abdul Hanan2* and Sher Ahmed2

...rms (6.33). However, the saffron stigma fresh and dry weight produced by large corms was recorded as 0.0354 and 0.0075g, respectively. Further results indicated no significant differences among the parameters recorded for the row to row and plant to plant spacing experiment except for total flower production where the highest production (162.3) was recorded in 15 cm spacing. The results also indicated that corms with a weight of 8-11 g are suitable for sowing ...

Imran Ahmad

... To evaluate the various samples, a focus group of twelve panelists on the same day of preparation using a 9-point hedonic scale for seven attributes: viscosity, airiness, graininess, consistency, smoothness, adhesiveness, and color intensity. MIS concentrations at 3-5% were not easily detectable by panelists. However, 7% MIS products were more desirable to panelists than the control. Based on Textural Profile Analysis, MIS ...

Sar Zamin Khan1*, Muhammad Waqas1 and Sagar M. Goyal2

...Certain serotypes have disappeared because of the availability and use of vaccines but new serotypes have emerged. A regular watch on the disease including virus variation, prevalence, pathogenesis and development of diagnostic tools are important for the formulation of effective prevention and control programs. This review deals with the global prevalence of IBV along with its pathogenesis, diagnosis, and control. In summary, we have discovered that measures ...

Syed Muhammad1*, Badar Naseem Siddiqui2, Farhat Ullah Khan1, Muhammad Adnan3, Sami Ullah3 and Nawab Khan4

...ardan, Upper Dir and Charsadda), and all public extension field Staff (field assistants (FAs) and Agriculture Officers (AOs) working in the above areas constituted the sample respondents.Data were analyzed using Statistical Package for Social Sciences(SPSS). The results showed that the technical competency of FAs remained in between low and high categories with mean values ranging from 2.45 to 3.80. Similarly, the technical ...

Abdul Hassan1*, Arshad Farooq1, Muhammad Ishaq2, Ghulam Sadiq2 and Asif Nawaz3

...stricts Nowshera and Charsadda of Khyber Pakhtunkhwa, Pakistan using principal component analysis approach and regression analysis. The data were collected randomly from 130 sampled vegetable growers comprised of 65 respondents each from Nowshera and Charsadda districts through pre-tested interview schedule during financial year 2020-21. Principal Component Analysis was used for determinin...

Munawar Lal1*, Naeem Tariq Narejo2, Muhammad Hanif Chandio1, Faheem Saddar3, Hameeda Narejo4, Ghulam Dastagir5, Ghulam Abbas6 and Shahnaz Rashid6

...an, was nearly ideal and satisfactory. The relative condition factor (Kn) was found to be fluctuating between 0.98 and 1.01 in all five dominant species. Results indicated that the values of length-weight and coefficient of condition analysis were ideal and growth of five dominant species was satisfactory from Nurri Lake District Badin, Sindh, Pakistan.

...

Ahamed Mohamed Korayem, Moawad Mohamed Mohamed* and Mohamed and Mostafa Mohamed Attia Hamman

...020. A total of 100 root samples were collected and processed for identification of root-knot nematode species. Data revealed the presence of three root-knot nematode species, they were Meloidogyne incognita, M. javanica and M. arenaria. The former species was the most frequent (58.8%) followed by M. javanica (31.9%) and M. arenaria (9.4%). Six Egyptian soybean cultivars; Giza-21, Giza-22, Giza-35, Giza-82, Giza-83 and Giza-111 were evaluated for their resista...

Nawfal Hammadi Jasim1, Yasmeen Jassim Mohammed2 , Jala Amir Salman Alahmed1, Majdy Faisal Majeed3* 

...roup 2 received daily dosages of methylphenidate in escalating amounts (5, 8, 12, and 16 mg/kg/day in a 5% of sucrose solution), as well as Group 3 as control animals ( 5% glucose solution). Group 1 resembled the therapeutic doses administered to humans, while Group 2 simulated the wrong use of some of the human addicts. 30 days after the last Ritalin dose administration, the rats were all sacrificed, and blood and liver ti...

Saima Mehar1, Amtul Sami2 and Erum Iqbal3*

...inum cyminum and Nigella sativa) in methanol, chloroform, ether, butanol, and hexane on egg hatching and mortality of the juveniles of root-knot nematode Meloidogyne javanica. After 72 hours, C. cyminum (MeOH) demonstrated the maximum nematicidal activity in egg hatching (15%±2.10) of M. javanica, followed by N. sativa (Ether), 22.33±6.86%, at the lowest administered dose of 0.25 % concentration. After 72 hours...

Sumbal Nazir1*, Farkhanda Manzoor2, Ishrat Perveen3, Anum Rana2, Irum Naureen1, Abad Ali Nadeem3 and Hafiza Najma Naeem2 

Muhammad Mukhtar1*, Lely Ummi Wakhidah2,Mohammad Zubair Hippy3 

...protein. Thus it is necessary to add a strategy to the previous strategy (strategy) in the development of beef cattle in Gorontalo. Based on the results of the AHP and SWOT above, it can be seen that the implementation of all process models for the beef cattle development policy strategy is the WO strategy. The level of the role of the actor element on the focus element found that the actor who has the biggest role in the development of beef cattle in increasi...

Indira Jurinskaya1*, Tynyshtyk Kenzhebaeva2, Kairat Iskakov3, Bekzat Niyazbekov1 

... purpose of the reconnaissance experiment was a series of preliminary studies aimed at breeding aspects of increasing the down output of goats of local breeds and reducing the cost of manual labor in the technology for obtaining down. The research was carried out at the “Aiganym” farm of the Zhambyl district on one-year-old goats of the Kazakh coarse-haired and Gorno-Altai downy breeds as well as their crossbreeds. Animal groups were formed accordi...

Pawinee Kingkan1, Thanathip Supcharoenkul1, Chaowit Rakangthong1, Chaiyapoom Bunchasak1, Komwit Surachat2,3, Wiriya Loongyai1* 

...d to two treatments: a basal diet supplemented with a mixture of amoxicillin and colistin (Amox-Co) and a basal diet supplemented with amoxicillin and 1 g/kg of a bacteriophage cocktail (Amox-Phage). Each treatment consisted of eight replicate pens, with 50 pigs per replicate. Average daily gain (ADG) and the FCR did not differ between the groups. The Amox-Phage group showed greater resistance to diarrhoea compared with the ...

Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1 

...as detected in the brood sample of batch 1 and batch 4, respectively. The variations in Aeromonas and Pseudomonas counts among different batches of different components were statistically non-significant (p>0.05). While, most of the components along the larval production line had significantly (p<0.05) varied Vibrio counts for different batches. Larval feeds were found mostly contaminated with pathogenic bacteria, especially with pathogenic Vibrio in the...

Muhammad Waqas Yonas1,2*, Khuram Mubeen2, Muhammad Irfan3, Muhammad Ayaz Shahzad4, Mudassir Aziz2, Saad ur Rehman5 and Shoaib Zawar

...2: stale seedbed + glyphosate @ 711 a.i mL ha-1, T3: stale seedbed + paraquat-dichloride @ 494 a.i mL ha-1, T4: stale seedbed + atlantis (mesosulfuron-methyl and iodosulfuron-methyl-sodium) @ 15 a.i g ha-1, T5: stale seedbed + glyphosate @ 356 a.i mL ha-1 + paraquat @ 247 a.i mL ha-1, T6: stale seedbed + glyphosate @ 356 a.i mL ha-1 + atlantis @ 7.5 a.i g ha-1, T7: stale seedbed + paraquat...

Muhammad Zeeshan Siddiqui1, Mujahid Ali2*, Shoaib ur Rehman3, Shahid Iqbal4, Malik Abdur Rehman5, Hafiz M. Tayyab Khan4, Muhammad Ali6, Muhammad Azher Nawaz4 and Saqib Ayyub1

...s i.e., Green ball, Dilpasand, Durga, Round gold, and Indian desi were sown. One month after emergence, the growing medium was supplied with the lowest NaCl level 2, two medium levels 4, 6, and the highest salinity level was 8 dS m-1 and was compared with the control (1.5 dS m-1 considered as normal). The Hoagland solution was applied every week as a nutrient solution. High sodium contents lead to sodicity. Finally, selected...

Jaffar Hussain1*, Zeenat M. Ali1, Syed Farman Ali Shah1, Abdul Nasir Laghari2 and Munazza Sohail3

...ion annual yield 200 thousand metric tones. Suitable to many site conditions in arid and semi-arid areas of the country. The 70 to 80% directly or indirectly Pakistani populations depend upon agriculture. Jatropha oil have density 909kg/m3,viscosity 25.63CST,flash point 250 oC and acid value was 1.20 mgKoH/g.

...

Asim Zubair1*, Ayesha Aziz2, Ghulam Akbar Malik3, Iffat Batool4 and Zohaib Atta Mehdie5

...respondents using random sampling technique. The data that had been gathered, was examined using SPSS. According to the findings of our research, women in the study area were energetically involved in agricultural activities. To better meet the requirements of their family, they invested more time in agricultural activities.

...

Bahlouli Fayçal1*, Chourghal Nacira2, Salamani Amel3, Benaini Mohammed4, Maamri Khelifa2, Kachaou Cherifa5, Djaballah Melak5, Atek Younes6 and Aissat Fares6

Martin-Luther Oseni Okolo1, Monday Eneojo Akor1, Cornelius Arome Omatola1*, Zainab Penninah Ochada1, Ugbane Eleojo Okolo2, Benjamin Mudi Idache3, Andrew Isiramen4, Julius Akor Omatola5 and Moses Adaji David6

...ing Xpert® MTB/RIF Assay Version 4. The prevalence of HIV/TB was found to be 6 (1.85%). Infection was more prevalent in the age group 26-35 years (3.92%) and among the females (83%) than their counterpart males (17%). Co-infection was found only among the married (2.43%). A higher prevalence (1.89%) was found in patients from monogamous families (2.41%) and in patients residing in rural areas compared to urban dwellers (1.65%). Occupation-specific prevalen...

Budi Guntoro1*, Agung Triatmojo1, Bambang Ariyadi2, Nguyen Hoang Qui1 

...ing a multi-stage random sample method and quota sampling. Descriptive statistics were used to analyse primary data from the survey. Farmers were chosen by the criteria of who are staying and raising beef cattle in Yogyakarta. The results showed that farmers had a high experience and more than 50 years old, they worked as a farmer at the farm with low income from cattle production (less than 100$). Farmers had more than 8 ye...

Winda Sartika1*, Budi Hartono2, Hari Dwi Utami2, Lilik Eka Radiati2 

... survey method, snowball sampling in the rendang industry with raw beef, and purposive and accidental sampling on the supply chain institutions involved in rendang production. The research respondents comprised 61 respondents from the rendang industry, including suppliers of raw materials, distributors, and consumers of rendang with qualitative and quantitative descriptive data analysis. The results of the study show that th...

Jawad Ali1,*, Samiya Rehman1, Aqsa Aslam1, Fouzia Tanvir2, Rida Liaqat1, Muhammad Ahmad1, Aamir Riaz1, Muhammad Shafique3, Hassan Ali1 and Amjad Ali1

...hypolipidemic, antidepressant, immunosuppressive, anti-microbial properties to the W. coagulans Dunal. Plant samples from Mianwalli, Kohat, and Sargodha city were gathered for antioxidant potential determination. Free radical scavenging activity and total antioxidant activity experiments were performed on the samples using the DPPH and Phosphomolybdenum methods. Results shows leaves extrac...

Muhammad Usman Shafi1, Hafiz Azhar Ali Khan1*, Tiyyabah Khan2, Waheed Anwar2, Adnan Akhter2 and Muhammad Zubair3

...pesticide handling, disposal, storage and Personal Protection Equipment (PPE) during operation leads to health problems of human, increase resistance against pests with negative effects on the environment. The purpose of this survey was to check the knowledge about pesticide uses, risks and storage among farmers in Lahore division of Punjab, Pakistan. And, to get knowledge from farmers regarding local knowledge system (LKS) and Integrated Pest Management (IPM)...

Tasew Getu1*, Wassu Mohammed2, Awol Seid3, Tesfamariam Mekete4 and Bekele Kassa

...s (2.6 × 105) with same treatment. The presence of VC greatly increased tuber yield, and Guassa and Bubu produced the highest marketable tuber yield of 47 and 48 t/ha, respectively under this treatment. All cultivars that were grown with drip irrigation amended with VC fell into the resistant category towards MI and RS disease complex. The study revealed that the tested treatments respond differently to the disease com...
Muhammad Zahid Rashid1, Amina1*, Hafiz Wasif Javaad2, Muhammad Asim Rashid3 and Amina Rashid3
...limatic conditions of Faisalabad. Different vegetative parameters such as, No. of days taken to start of germination/survival percentage, number of fronds, number of seeds survived, plant height, leaf area, suckers evolved and other parameters were noted for better yield and quality of date palm for purpose of chance seedlings development. This research experiment was arranged at Progeny orchard of Horticultural Research Institute, Fai...

Imtiaz Khan1*, Bashir Ahmad1, Ahmad ur Rahman Saljoqi1, Shah Alam Khan1, Javed Khan3 and Muhammad Azim Khan2

... sugarcane crop. For the safe management of P. perpusilla, field experiments were conducted to find the population of Pyrilla perpusilla on 12 different sugarcane varieties under field conditions at two different districts Charsadda and district Dera Ismail Khan of Khyber Pakhtunkhwa. Plot size 36×16 m (length × width) was selected in both localities for the cultivation of sugarcane varieties (CP77/400, Jhang 59,...

Asmaa A. Darwish*, Adel M. El-Kattan, Mona A. Mahmoud, Mohamed T. Ragab, Amani A. Hafez 

...gical examination. Blood samples were collected for immunological, hormonal, and iron profile estimation. The total recovery of B. melitensis was 13.50% with nearly equal percentages in sheep (13.60%), and goat (13.33%). B. melitensis isolates had bvfA gene, virB gene, and ure gene with percentages of 62.8%, 77%, and 37% respectively. B. melitensis infection in sheep and goat resulted in a significant (P˂0.05) increase in the pro-inflammatory cytokines, acute...

Abdulaziz A. Alaqil

...groups have received a basal diet with no PR supplements, whereas the PR and PR+EC groups have received PR at 1 g/kg of the basal diet. On day 36 of age, the EC and PR+EC groups were injected with 0.5 mL intraperitoneal (i.p.) suspension contained O157:H7 EC strain (adjusted at 107 CFU/bird), while the C and PR groups were injected i.p. with 0.5 mL saline only. All data were analyzed using...

Thekra Fadel Saleh, Omar Younis Altaey*

...ons. The density of glycosaminoglycans was also higher in goblet cells at the basal crypts compared to longitudinal crypts. Immunohistochemical analysis revealed higher levels of lymph follicles and B lymphocytes in the proximal portion compared to the middle and distal portions. The complex architecture suggests that caeca in Muscovy duck play a significant role in microbial digestion and immunological functionality and Ins...

Peng Xu1, Yankuo Li2*, Shusong Zhang1 and Bin Liu3

...trapped at nine selected sampling sites varying in altitude for evaluating their diversity. Consequently, 16 rodent species belonging to eight families were recorded. Out of 376 individuals of rodent species trapped (capture rate = 4.7%), Apodemus agrarius, Eothenomys melanogaster, Niviventer fulvescens and N. confucicianus were identified as dominant species with relative abundances over 10%, in which N. fulvescens and N. confucicianus were trapped in eight a...

Fakhra Nazir1*, Sahar Fazal1 and Fakhar-i-Abbas2

...subunit I) was aimed for samples of birds collected from different regions of Pakistan. Mitochondrial DNA was successfully extracted from keel tissue and Folmer region of COI gene comprising ~650 bps was amplified using universal primers and PCR products were confirmed by 1% agarose gel electrophoresis. Sequencing was carried out by Sanger’s method and BLAST analysis identified these sa<...
Nasir Ali Tauqir1*, Asim Faraz2, Abdul Waheed2, Ayman Balla Mustafa3, Irfan Shahzad Sheikh4, Michela Pugliese5 and Shahid Nazir6
... nitrogen balance. Blood samples were collected through jugular vein two hours post feeding and were analysed for blood triglycerides concentration, total proteins and blood urea nitrogen according to standard procedures. Nutrient intake, nutrient digestion, nitrogen balance, milk production, milk fat, total solids in milk and solid not fat did not show any treatment effect. However, milk protein percentage was the highest (3.42% and 3.41%) in buffaloes fed di...

Md Tariqul Islam1,5 *, Aditya Chowdhury Avi1,5, Md Saiful Bari2,5, Most Rebeka Sultana Swapna Khandoker3, Homaira Pervin Heema4, Goutam Buddha Das1 

Meltem Kızıl1*, Ali Rişvanlı2, Murat Abay3, Tarık Şafak4, Mehmet Akif Kılınç5, Öznur Yılmaz6, Burak Fatih Yüksel1 and İbrahim Şeker1

...n from these cows. Blood samples were taken from cows and calves in all groups during birth, after drinking colostrum from calves and on the 15th, 30th, 45th and 60th days after birth in 10 ml tubes. The levels of the aforementioned molecules in these blood samples were determined by ELISA method. Feeding with colostrum led to a decrease in the irisin le...
Umara Amir ud Din1, Waqas Ahmed Khan1*, Khurrum Shahzad1,2, Basharat Ali3, Misbah Hussain1,4 and Fazli Rabbi Awan4
...rphism was done by PCR assay. Statistical analysis showed that subjects with DN had higher (67%) frequency of ID genotypes and the ID genotype carriers also exhibited higher levels of creatinine (6.2±3.9 mg/dL) and urea (99±41 mg/dL). Moreover, logistic regression analysis also indicated that ACE ID genotype carriers had a 2.5-fold higher risk of DN as compared to the II genotype carriers. Hence the present study shows a strong association (OR 2....

Suleman Irfan1*, Masood Rabbani1, Ali Ahmad Sheikh1, Sehrish Firyal2 and Arfat Yousaf Shaheen1

... serovars. A total of 80 samples, comprising of poultry meat (n=30) from poultry shops and supermarkets, poultry feed (n=30) and swabs from carcasses and muddy (n=20) of poultry shops. The samples were assessed microbiologically for Total Viable Count, Total Coliform Count, and Salmonella detection. The mean log values of total viable counts of meat samples of traditional poultry shops, su...

Khalil1*, Dwi Ananta1, Andri Bachtiar2, Hermon3

...ur experimental diets: basal diet + fresh rice straw (FRS), basal diet + stacking-supplemented rice straw (SSRS), basal diet +rolling-supplemented rice straw (RSRS), and basal diet + wrapping-supplemented rice straw (WSRS). The diets were fed. Parameters measured included: live weight gain (LWG), dry matter intake (DMI), feed conversion ratio (FCR), and ...

Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2

... dose). Liver and kidney samples were collected from all mice during different experiment periods, kept in 10% neutral buffered formalin, and sent for histological analysis. No abnormal histological features were seen in liver and kidney sections from the control and other mice groups before various treatments. The various treatment groups revealed diverse histopathological changes compared to the control groups. Various histological features were also seen in...

Mohamed Adam1*, Hassan Sobhy2, Mohamed Abouzid3, Dalia Elhafny4 and El-Desouki Ibrahim5

...application in 2016. The same trend in the control potential for each treatment was also obtained in 2017 experiment. The residues of ethoprophos and fenamiphos in leaves and fruits were not detected during the whole experiment. The amounts of ethoprophos and fenamiphos decreased from zero to 5 days from (4.88 to 3.61 ppm) and (15.25 to 7.31 ppm) with loss of 26.02% and 47.93 %, respectively. For all tested products, no impacts on Chlorophyll degree in leaves ...

Mati Ullah1*, Muhammad Rizwan2, Ali Raza3, Xianlin Zhao4, Yanling Sun4, Sarah Gul5, Muhammad Ihtesham Waheed6, Muhammad Nadeem Khan7, Alvina Gul8, Sami Ullah Jan9* and Chao Huang4*

... from traditional yogurt samples using the next-generation sequencing approach. The GC contents of the characterized strains were in the range of 46.03 to 46.52% while the protein-coding sequences (CDS) were in the range of 2544 to 2888. The pan-genome analysis of the three strains identified a total of 5449 genes with 377 as core genes. The functional characterization by subsystem category distribution analysis revealed “Carbohydrates” is the larg...

Imtiaz Kashani and Sher Khan Panhwar*

... ponyfish, Karalla daura sampled from Keti Bunder, Karachi Fish Harbour, Hawks Bay and Pasni along the coastal belt of Pakistan. Size frequency distribution based on normal distribution demonstrates that individuals of 13 cm total length were common in all groups. Some fifteen morphometric and six geometric variables were used to develop Principal Component Analysis (PCA) that revealed (76%, 13.9% and 9.8%) variation deduced from first three components. The po...

Ruobing Han, Shanshan Gui, Xunwu Zhao and Heping Li*

...lation. We used ten microsatellite loci to genotype all 28 white-red deer individuals in China, and constructed a Neighbor-joining (NJ) tree based on genetic distances. In addition, genetic structure analysis was carried out. The results showed that a total of 67 alleles were detected, and the average number of alleles at each locus was 6.7. There was high genetic diversity but severe inbreeding in the white-red deer population, and there is no father-mother-o...

Falah Mahmood Hameed1*, Asaad Khalaf Talal AL-Shuwaili2, Eman Jawad Jabber3, Mayada Sahib Hassan1  

...y was to assess how chitosan affects the capacity of rabbits to heal full-thickness cutaneous lesions, 24 clinically healthy adult male rabbits weighing 1.5 to 2 kg were utilized in the experiment. The interplay between intramuscular of 5 mg/kg xylazine hydrochloride, 35 mg/kg ketamine hydrochloride, and 1 mg/kg diazepam were carried out. Based on the sort of treatment applied, these animals were allocated into three groups (A, B, and C). Chito

Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin 

...s included; adult granulosa cell tumor (0.55 %), interstitial cell tumor (0.55 %), thecoma or fibrothecoma (1.10 %), steroid cell tumor-NOS (0.55 %), and granulosa-theca cell tumor (5.55 %). Specifically, all cases had distinctive histomorphological appearance characteristics for benign tumors, except adult granulosa cell tumor, which had sarcomatous cha...

Khalid Mahmood1*, Arslan Lodhi1, Muhammad Asim2, Muhammad Adeeb Babar3 and Muhammad Akbar Khan1 

...agoportax punjabicus, T. salmontanus, and Pachyportax latidens. The presence of these bovid species indicates moderate taxonomic diversity of bovid fauna in the Dhok Pathan region of the Siwaliks of Pakistan.

...

Indri Juliyarsi1*, Sri Melia1, Rizki Dwi Setiawan1, Deni Novia1, Lailatul Anshor2, Tomi Candra2 

... 0.6%. 

...

Ikram Ullah1, Zaib Ullah2*, Junaid Khan1, Sajid Mahmood1, Zafar Iqbal3 and Naveed Akhtar2

...black bear damage, compensation of loss and community involvement in conservation programs were documented as the most effective mitigation strategies. Human dependence on forest resources, habitat destruction, anthropogenic food waste, and retaliatory killing were the main conservation threats to black bear survival in the study area.

...

Amr N. El-Shahat1*, A.M. Abdul Azeem1 and Mohamed H.M. Abd el Megid2

....8-cineole, Linalool and sabinene which represent the main volatile oils of bay leaf. Supplementation of BLE with HCD for 10 weeks resulted in reduction in the levels of some lipid contents (total cholesterol, triglycerides, Low-density lipoprotein-cholesterol and very Low-density lipoprotein-cholesterol), tumor necrotic factor-alpha and interleukin-6 as well as reduced activity of hepatic and cardiac enzymes. Also, feeding rats on HCD with BLE enhance the ant...

Sarwat Yousuf1, Shaista Emad2 , Mohammad Misbah ur Rehman3, Zehra Batool4, Sara Qadeer5, Yousra Sarfaraz1, Sheeza Sheikh1, Sana Sadaf6 and Tahira Perveen1,*

... low doses has antidepressant and anxiolytic activity. Muscular strength was also improved at low doses. The brain antioxidant defense enzymes were also enhanced whereas plasma corticosterone levels were significantly decreased following the administration of a low dose of lemon peel oil. However, rats administered with higher doses of lemon peel oil that act as prooxidants showed depression and anxiety-like effects, and impaired muscular strength. Altered bra...

Yufang Liu1,2, Guiling Cao3, Yujing Xie3 and Mingxing Chu1*

... 180d, BO group) and the same age control of puberty (JG 90d vs. LC 90d, EO group). Ovary tissues were taken from goats on 30 days, 90 days and 180 days, total RNA was extracted, differentially expressed (DE) gene libraries were constructed by suppression subtractive hybridization (SSH) technology, and puberty-related differential genes were screened. A total of 184 differentially expressed genes were screened, including 57, 68 and 59 in the AO, BO and EO grou...

Yajuan Zheng, Qiuping Mo, Hongchao Tang, Qinghui Zheng and Dandan Guan*

...Fresh breast cancer case samples and normal breast cell samples were collected. Western blot was used to analyze the expression of LGR5 and (phosphorylated IQGAP1)/(IQGAP1). Construct metastatic human breast cancer MDA-MB-231 cell line knocking down LGR5. The relative expression levels of LGR5 and phosphorylated IQGAP1 were detected by Western blot. The relative activity of the above three types of cells after 24 h of cultur...

Abdul Zahir1*, Asad Ullah1, Muhammad Jawad1 and Syed Attaullah Shah2

...er uses rights and their satisfaction with irrigation water distribution were assessed. For this purpose, 466 farmers were randomly selected through a multi stage stratified random sampling technique from three irrigation sections in District Malakand, District Charsadda, and District Mardan, Khyber Pakhtunkhwa (KP). This study revealed that majority of the respondents did not attend forma...

Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1

...ctious illnesses. Allium sativum is being used traditionally since ages for many purposes. It is extensively used in numerous dishes throughout the world and due to its aromatic nature, as a fragrance and Plantago ovata is traditionally used for many therapeutic purposes; laxative anti-acidic stabliser, stomach soothing, diuretic; and it is also anticancerous. Nigela sativa is used for jaundice, paralysis, metabolic syndrome...

Ahmad Noor and Malik Muhammad Shafi*

...Jangerizi Banda, Yaghi Musakan, Hassan Banda, Takht Nasrati and Garang. Data were collected through questionnaires, while secondary data was from different climate and weather reports. Binary Logistic model was used for analysis. The study discovered lack of surface water resources and ground water extraction satisfied the community needs and rainfall was only recharge method. Boreholes pu...

Monis Hussain Shah1*, Riaz Ur Rehman2, Riaz Ali Shah1, Farwa Batool3, Rizwan Rafique4, Muhammad Usman5, Sajida Bibi6, Sadia Yasin7 and Samida Qamar8

...t famous in Pakistan for sale and cultivation for commercial purposes as well. The evaluation of exotic varieties are very important for recommendation to local farmers for better yield. Four tulip varieties cv. Antarctica, Oxford, Ballerina and Apeldoorn were purchased for evaluation. The 100 bulbs of each variety were planted in the bed. The performance of the plants during 2017-18 were judged by observing the bulb germination percentage, leaf width (cm), le...

Skenndri Safae1*, Charrat Nadia2, Jmiai Mehdi3, Nassik Saadia1 

...e Doukkala area of the Casablanca-Settat region and the health of the digestive tract of the birds. The 18 farms were firstly categorized into three (3) groups according to their performances on a biosecurity level: P group for “poor” performances, D group for “decent” performances, and G group for “good” performances. Each farm was then sampled twice on day 11 and day 35 of the

Yaman Saad Fadhil

...extract concentration in samples leads to a reduction in the growth rate of the bacterial population, and it continued till the last day of storage at 4oC. The sensory study revealed that odour alterations in samples that contain propolis extract were much better in treated samples compared with the control treatment. This study concludes that propolis water extract has potential applicati...

Dina A. Yousif, Mohamed M.T. Emara, Marwa R.S. Abdallah*  

... the plain marinade. All samples were marinated for 24 h at 4 °C before being cooked to a final core temperature of 75 °C using either moist or dry thermal processing. The fatty acid profile, pH value, objective color indexes, sensory characteristics, and marinade uptake were all measured in the raw mutton samples. Additionally, the cooked samples were examined for cooking loss, fa...

Antonius Agung Wiono1, Maria Elizabeth Nana Wijayanti1, Dhika Yonika Primacitra1, Trioso Purnawarman2, Andriyanto3, Aulia Andi Mustika3, Amaq Fadholly3* 

...and lowest mortality ratesamong the groups. While, the triglycerides, cholesterol, LDL and cortisolvalues were lower compared to others group, also the data of SGPT and SGOT values were similar to normal control. Conclusion: The data showed that PhytoceeTM 200 g/ton of feed had the best results among the groups.This study inferred that PhytoceeTM 200 g/ton of feed can mitigatesthe adverse effect of heat stress and enhances the productivity of broiler chicken e...

Yanping Li, Fei Xu, Yongming Wang*, Yunyun Lv, Jinrong Shi, Biwen Xie, Wenyao Cai and Dian Liu

...uperciliaris and S. reevesae are two economically important loaches in the middle and upper reaches of Yangtze River. Recently, wild populations of them have dropped sharply due to anthropogenic and environmental threats. There is an increasing need to implement captive propagation programs to protect their populations. Here, we firstly carried out artificial breeding of S. superciliaris and S. reevesae, and hybrid combinati...

Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*

...at and Saanen goat. Milk samples were collected during 2021. The main characteristics and constituents of milk including colour, odour, taste, freezing point, dry matter free of fat, acidity (lactic acid), density (in 15 °C), pH, fat and protein were determined according to the standard methods. Macro and micro elements of milk were analyzed using inductively coupled plasma mass spectrometry. Data were subjected to the two-way ANOVA analysis to determine t...
Daud Khan1, Naila Chand1, Muhammad Saeed2, Muhammad Tahir3, Alam Zeb4 and Rifat Ullah Khan5*
...e control (birds fed a basal diet and not challenged), positive control (birds fed a basal diet and orally challenged with E. coli O157 H7 at the rate of 1 × 109 CFU/ml; positive control), infected + enrofloxacin, infected + silver nitrate at the rate of 100 mg/kg (SN100) and infected + silver nitrate at the rate of 150 mg/kg (SN150). Overall feed intake, body weight gain, feed conversion ratio (FCR) and dressing perce...

Xiaoyan Yang1* and Xiuli Xu2

...virus that attacks the nasal passages, including the throat and lungs. Influenza-like virus in adults is partly due to changes in the body’s defense system. The present study was performed to evaluate influenza-like virus effects in adults. This case-control study was performed on 50 patients admitted to the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine during the autumn and winter seaso...

Asifa Hameed1*, Haider Karar2, Abdul Ghaffar1, Abid Hameed Khan1, Muhammad Mubashir1 and Ghulam Mustafa1

...etaul No.12, and SB Chaunsa) and two treatments (netted and open trees). The netted trees of each variety were covered with mosquito net before initiation of flowering, while the open trees of the same varieties were not covered with net. Overall, we found that insects are necessary for pollination and fruit setting. There was no fruit setting i.e. zero fruits per panicle in netted trees w...

Muhammad Aijaz1, Nasir Mahmood2, Ghulam Mujtaba3 and Imran Riaz Malik1*

...m some randomly selected samples. Urban locality, drinking of non-filtered water, poor hygiene, and poor socioeconomic status are the risk factors for the onset of breast cancer in the Pakistani population. Molecular analysis of the human IL-10 gene revealed that the prevalence of homozygous normal GG allele was 19%, heterozygous GA was 29% and homozygous mutant AA was 52% in breast cancer patients. However, in healthy individuals, GG allele was found in 70%, ...
Mahroze Fatima1*, Syed Zakir Hussain Shah2, Muhammad Afzal3, Muhammad Bilal4 and Ayesha Khizar1
...enic acid), 20:5n-3 (eicosapentaenoic acid), 22:5n-3 (docosapentaenoic acid), 22:6n-3 (docosahexaenoic acid), total n-3 and n-6 polyunsaturated fatty acids were found higher in diets enriched with vitamin E than in the control group. To conclude, dietary supplementation with vitamin E has been shown to improve the growth and antioxidant status of fish. N...
Muhammad Rizwan1, Hamid Akbar1*, Aftab Ahmad Anjum1, Muhammad Arif Khan1, Aneela Zameer Durrani2, Muhammad Abid Hayat3, Anjum Masood4, Muhammad Talha Sajjad1 and Nadeem Raza2
...Dirksen technique. Blood samples of both groups were collected on days 0, 7, 14, 21, and 28. Levels of various oxidative, metabolic and hematological parameters were measured by authenticated standard methods. We observed that serum levels of MDA and BHBA were higher at day 0 to day 14 (P<0.01), while the level of nonesterified fatty acid (NEFA) was higher at day 0 to day 7 (P<0.01) which dropped down to normal at day 21 (P>0.05) post-surgery in Group...

Trinil Susilawati1*, Aulia Puspita Anugra Yekti1, Amir Firdaus2, Dinda Ayu Damayanti2, Rizki Prafitri1, Nanang Febrianto1, Kuswati1, Achadiah Rachmawati1, Sri Wahjuningsih1, Nurul Isnaini1 

...on was repeated with the same treatment and then continued to observe NRR 2 on the next cycle. After two months, the pregnancy and AI failure were observed using ultrasound. A Chi Square was performed using SAS OnDemand for Academics (ODA, Cary, NC, USA). Moreover, probability values were calculated using the least sig...

Dalia M. Amin1*, Noha Mohammed Halloull1, Walaa E. Omar2, Basma A. Ibrahim2, Nahla M. Ibrahim1 

... (CRD). Thus, it is necessary to search for beneficial and helpful medications that can stop CRD from progressing to end-stage renal failure. Aims: The purpose of this study was to evaluate how erlotinib and garcinia cambogia alleviate the harmful effects of adenine on the rat’s kidneys. Methods: Forty animals were divided into four groups: Control group: received normal vehicle, adenine group: received orally (50 mg/kg/day) that is subdivided into ...

Siswanto Imam Santoso*, Agus Setiadi, Wahyu Dyah Prastiwi 

...duck farming, it is necessary to increase the role of the farmer association, apply feed processing technology, diversify duck egg processing and boost technical efficiency in regard to increasing duck egg production. Focusing on these efforts will increase the sustainability of duck farming in Indonesia. Duck farming is sustainable based on a social perspective, economic analysis, and environmental viability. 

...

Aneela Ahmed Tagar1*, Mahvish J. Channa2, Kanwal Ahmed Tagar3 and Kaka Kainat4

...f patients whereas blood samples were collected to detect the biomarkers (Ferritin, D-dimer, C- reactive protein, LDH, CBC and troponin). The study reveals the following findings as majority of patients were in age group 37-47 years, male and were married. Moreover, the majority belonged to the medical field. The greater number of the patients were physically active. Only 18.5 % of patients reported co-morbidities such as diabetes in 12.3% with one exceptional...

Abdulaziz A. Alaqil*

...sly injection of lipopolysaccharides (LPS). A total of one hundred twenty, 40-week-old, laying hens from the Hy-line breed were allocated at random into four groups of 30 hens each. For eight consecutive weeks (40–48 weeks of age), two groups of the layers were given a basal diet as control, while the remaining two groups were given a basal diet enhanced with a 2 g/kg diet of LA...

Mehwish Malik1*, Zanib Sadia2, Muhammad Sajid3, Hammidullah1, Yasir Amin1, Zubair Ali1 and Sohail Ahmad1

...as noted that out of 210 samples 08 samples were found negative (no growth of any bacteria found) and 20 samples were shown growth other than E. coli suggesting a total prevalence of E. coli as 86.6 %. The prevalence of ESBLs in cecum of broilers in the present study is 53% of the total samples tested which is 61% of total positive ...

Desi Kurniati Agustina1,3, Suyadi Suyadi2*, Veronica Margaretha Ani Nugiartiningsih2, Kuswati Kuswati2 

... by the community. Blood samples were obtained from 60 heads of Madura and Limura (Limousine x Madura) cattle, which were differentiated by sex and age. Blood samples were collected into tubes and immediately stored in a cold box at approximately 4°C. Data analysis was conducted at the Veterinary Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia. A statistical analys...

Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova* 

...his study is to obtain dosage forms from plant raw materials Calligonum leucocladum, to study their pharmacotherapeutic activity and use for the treatment of endometritis in cows. A total of 270 cows were examined by clinical methods after calving, of which 45 cows showed signs of acute endometritis. Subsequently, the bacterial background of uterine mucus in sick cows was investigated and St. pyogenes, E. faecalis, and E. coli were isolated. For the complex tr...

Muhammad Hasnain1*, Muhammad Hussnain Babar1, Ghulam Sarwar1, Muhammad Kashif Nadeem2, Sajid Nadeem3, Muhammad Akram4, Ali Raza4, Mussurrat Hussain5, Qaisar Abbas5 and Muhammad Shahid4

...acaricides were used knapsack sprayer. Results showed a significant mite reductionby allacaricides as compared to control. Among the tested acaricides, hexythiazon 10% WP (Nissorun) gave the highest reduction of mites (i.e., 41.42, 55.56 and 37.90%) after 3, 5, and 7 days of first spray, respectively. Similarly, after second spray, hexythiazon 10% WP (Nissorun) again gave maximum reduction (i.e., 41.65, 39.33 and 16.40%) of T. urticae at 3, 5 and 7 days, respe...

Altaf Ur Rahman1, Muhammad Ajmal Khan2*, Ali Hazrat3, Awais Farid4, Muhammad Yahya3* and Inam Ullah5

...picryl-hydrazyl (DPPH) assay (150 µg/ml). Results showed that the methanolic extract of C. jacquemontii is a rich source of many phytochemically active compounds such as alkaloids content (3.60%), flavonoids (3.40%) and terpenoids 2.30%. The methanolic extract of C. jacquemontii depicted promising antibacterial potential towards various tested bacterial strain in order of P. aeruginosa > E. coli > S. typhi > M...

Abdullah*, Imtiaz Khan, Muhammad Ishfaq Khan, Muhammad Ibrahim and Muhammad Fawad

..., Cyprus rotundus, Vicia sativa L. Ehrh., Cynodon dactylon L. Pers. and Fumaria indica Hausskn were all found in five locations around the district. The main species in the district emerged as A. tenuifolius Cav. The highest relative weed density (64.2%) and relative weed frequency (45.2%), respectively was noted for A. tenuifolius Cav. While, the lowest value of relative weed density (3.5%) and relative weed frequency (1.2%), respectively was documented for E...

Abdur Rauf1*, Muhammad Jawad1, Farooq Jan1, Muhammad Qayash2, Muhammad Yasin4, Samrin Gul5, Wisal Khan3, Ikramullah Khan1, Farkhanda Bibi1, Wajid Khan6, Tanweer Kumar6, Muhammad Arif6 and Khilwat Afridi7

...sta-17, Gulzar-19 and Pirsabak-19) at different sowing times of 15-days interval. A pooled analysis across the same environment revealed a significant difference (P ≥ 0.01) for all the parameters i.e., days to heading, days interval, plant height, flag leaf area, tillers m-2, spike length, spikelet spike-1, grain spike-1, thousand grain weight, grain yield and biological yield across th...

Shujaullah Khan1, Zahid Hussain1*, Haroon Khan1 and Omer Suha Uslu2

...weeding. The highest thousand-grain-weight, biological yield and grain yield were recorded in the hand weeding treatments. However, the results of hand weeding treatments were statistically at par with the treatments of artificial intelligence (robotic weeding). The artificial intelligence got the highest CBR followed by the broad-spectrum herbicide and hand weeding. It can be concluded that the artificial intelligence could prove to be an economical as well a...

Abdur Rauf1*, Farooq Jan1, Muhammad Qayash2, Zia-ul-Islam3, Rizwanullah1, Ikramullah Khan1, Muhammad Shuaib4, Muhammad Khalid5 and Samrin Gul6

...e presence of alkaloids, saponins, flavonoids, and phenols in the ethanolic crude extract.

...

Abdur Rauf1*, Muhammad Sadiq1, Farooq Jan1, Muhammad Qayash2, Wisal Khan3, Ikramullah Khan1, Khilwat Afridi4, Muhammad Shuaib5, Muhammad Khalid6 and Samrin Gul7

...arch Institute (CCRI) Pirsabak, Nowshera, Pakistan. A total of sixteen genotypes i.e., eight exotic and eight local germplasms were sown in a three-replication randomized complete block design. The different parameters were recorded including days to heading, flag leaf area, plant height, tillers per plant, spike length, spikelets per spike, grain yield per plant, biological yield per plant, and 1000-grain weight. The analysis of variance showed significant di...

Nguyen Thi Anh Thu1*, Ly Thi Thu Lan1, Nguyen Thuy Linh1, Chau Cong Dang1, Nguyen Hoang Phuc1, Tran Dinh Tuan1, Nguyen Trong Ngu2 

Umer Farooq1,2, Nimra Murtaza2, Abubakar Siddique1, Bilal Saleem1, Obaid Ur Rehman1, Nageen Zahra1, Muhammad Uzair1, Muhammad Naeem Riaz1,3* and Muhammad Ramzan Khan1*

Zaki Al-Hasawi1* and Reda Hassanine2

... sediment and fish liver samples from the unpolluted site, while their corresponding concentrations were significantly high in the polluted site. Mean concentrations of other metals (Mn, Fe, Ni, Cu, Zn) in water, sediment and fish liver samples from polluted site were significantly much higher than those from the unpolluted one. In contaminated fishes from polluted site, mean levels of blood biochemical parameters, serum liv...

Zaki Al-Hasawi1* and Reda Hassanine2

... sediment and fish liver samples from the unpolluted site, while their corresponding concentrations were significantly high in the polluted site. Mean concentrations of other metals (Mn, Fe, Ni, Cu, Zn) in water, sediment and fish liver samples from polluted site were significantly much higher than those from the unpolluted one. In contaminated fishes from polluted site, mean levels of blood biochemical parameters, serum liv...

Shazia Rasheed1,*, Ehsan Ullah Mengal1, Robina Manzoor2 and Azra Bano1

...of female, GSI values of samples ranged from 0.71 to 13.33% having a mean of 5.46 ± 3.25 (Mean ± SD).

...

Rizki Palupi*, Arfan Abrar, Fitri Nova Liya Lubis, Yuni Kurniati, Bianca Ikriza Octa Nadia Putri 

...sing a combination of cassava (Manihot esculenta) tuber and indigofera (Indigofera zollingeriana) leaves meal in rations on nutrient digestibility, hematological status and malondialdehyde levels in broiler chicken blood. The study was conducted experimentally with a completely randomized design, which consisted of 5 treatments and 5 replications. The treatment was the use of a combination of cassava tuber and indigofera l...

Luh Gde Sri Surya Heryani1*, Ni Luh Eka Setiasih1, Ni Nyoman Werdi Susari1, I Made Merdana1, I Gusti Ngurah Bagus Tri Laksana2, I Wayan Nico Fajar Gunawan2 

...pic characteristics. The samples include 50 Bali pigs that are intensively reared in Musi Village, Gerogak District, Buleleng Regency, Bali Province, Indonesia. The measurement data were analyzed using PCA to reduce and interpret the measured variables. The results showed that the average hip height and width, rump length, body length, shoulder height, chest width and depth, head width and length were 55.68cm, 17.53cm, 10.62cm, 67.40cm, 54.92cm, 17.81cm, 41.24...

Malik F.H. Ferdosi1*, Arshad Javaid2, Iqra Haider Khan2 and Muhammad Kaleem Naseem

...t. These included heptacosan-9-ol (15.01%), 2-hexanamine (13.28%), n-hexadecanoic acid (10.38%), eicosane (10.37%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (9.69%), cyclohexane, 1,1’-(2-propyl-1,3-propanediyl)bis-(8.81%), hexadecane-1,2-diol (6.34%), pentadecanoic acid, 14-methyl-, methyl ester (5.76%), methyl linolenate (5.38%), octadecanoic acid (4.04%), methyl (Z)-5,11,14,17-eicos...

Komal Javed1, Sohaib Muhammad1*, Zarghaam Khan1, Sehar Fatima1, Hassan Nawaz1, Mahrukh1, Tahira Khalid1 and Shariat Ullah2

Arshad Javaid1*, Iqra Haider Khan1, Malik F. H. Ferdosi2, Aneela Anwar3 and Mujahid Manzoor4 

...g compounds included docosane (7.68%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (6.92%), pentadecane (6.20%), cyclopentanone, dimethylhydrazone (5.93%), dotriacontane (5.91%), humulene (5.82%), octadecanoic acid (4.05%), cis-vaccenic acid (3.72%), heptadecane (3.38%), and heptadecane, 2,6,10,15-tetramethyl- (3.10%). Six compounds with peak areas below 3% were categorized as less abundant. The three major compounds in flower extract of I. carnea posse...

Farheen Shafique1, Shaukat Ali2*, Mahreen ul Hassan3, Saiqa Andleeb1

... selected through random sampling and questioned face to face using a predesigned, pretested questionnaire. The first portion of the questionnaire enquired about basic demographic data; the second half evaluated the participant’s knowledge about thalassemia. The third and fourth sections dealt with people’s perceptions and attitudes regarding thalassemia. The Statistical Package for Social Sciences (SPSS) Statistics was used to analyse the data. Ap...

Salman Ahmad1*, Muhammad Asim1, Muhammad Zeeshan Majeed2, Muhammad Atiq3, Yasir Ali4, Hafiz Bashir Ahmad5, Malik Abdul Rehman6 and Naeem Akhtar7

...eticulate cv. kinnow), musambi (Citrus sinensis cv. musambi) and feutrell’s early (Citrus reticulate cv. feutrell’s early) orchards was conducted in tehsil Sargodha during 2017 and 2018. Correlation and regression analysis were used to determine the relationship of ACP with CG, and the relationship of environmental factors with ACP. Surveys were conducted to record the incidence of CG and estimate the yield losse...

Sarfraz Ahmad1, Mian Anjum Murtaza1,* and Kashif Akram1,2

...ve (2% milk fat) control samples were also prepared for comparison. The Mozzarella cheese samples were analyzed for physico-chemical composition, functional properties, cheese yield and textural profile. The findings showed that there was a significant impact of added hydrocolloids (inulin and resistant starch) and their concentrations on the meltabilty, stretchability, hardness and yield of low fat mozzarella cheese. On the...

Abiyu Tadele 1,2*, Gebreyohannes Berhane2, Wondmeneh Esatu3

...the three genotypes were sampled. The findings revealed that, except for yolk breadth and color, the Tetra H genotype had considerably better (p < 0.05) egg quality characteristics than the other genotypes. Internal and external egg quality characteristics were found to have positive and significant associations among the genetic group of chickens, demonstrating that choosing birds with bigger eggs increases the proportion of edible eggs. As a result, our f...

Supawadee Piratae1*, Noraphat Khiewkham2, Nattawut Maungmungkun2, Chanakan Tippornwong2, Tossapol Seerintra2, Sirikanda Thanasuwan3, Luyen Thi Phung4 

...I: 15.9-31.1) of the dog samples in this population were positive for one or both pathogens. Of these dogs, 18.1% were infected with E. canis while 7% were infected with A. platys. Mixed infections were found in 2.4%. Infection with E. canis was significantly related to hematocrit, haemoglobin, red blood cell (RBC), mean corpuscular volume (MCV), and platelet levels (p < 0.05). In addition, there were significant differences in white blood cell (WBC) and pl...

Atef M. El-Sagheer1*, Aline F. Barros2, El-Sayed M. Abd El-Aal3, Mohamed M. Gad4, Doaa S. Mahmoud4 and Amr M. El-Marzoky3

...ssociated with banana (Musa cavendishii) and some of the most economically important ones are Radopholus similis and Meloidogyne spp. the purpose of this study is mainly to clarify the pathogenic effects of both nematodes (Radopholus similis and Meloidogyne incognita, alone or in combination), as a histological modification in root tissues under natural conditions of banana cultivation. R. similis was observed in the cortical parenchyma as a feeding site, and ...

Sumbul Zulfiqar and A.G. Rizwana*

...ments that are very necessary for human health. Diseases in fishes caused by invasion of parasite especially due to Philometra species is considered very much problematic in fish culture and aqua tilling. There are number of complications in fishes that arose due to presences of Philometra sp. Presence of these parasites directly affect fish reproduction and ultimately decline their populace. This research work was done to collect, observe and identify variety...

Farooq Jan1*, Abdur Rauf1, Ikramullah Khan1, Muhammad Yasin2, Muhammad Qayash3, Hazrat Wali1, Muhammad Luqman1, Fayaz Asad4 and Muhammad Khalid5

...n measured using RotoRod sampler. Many plants pollen are allergenic that cause different types of allergenic diseases globally. Pakistan also faces the problem of pollen allergy caused by various plants pollen. Among these allergenic pollen producing plants are Broussonetia papyrifera, Alternanthera pungens, Cannabis sativa, Eucalyptus globulus and Taraxacum officinales pollen grains are dominant. These plants pollen cause d...

Muhammad Adeel Farooq1, Shaukat Ali1*, Ali Hassan1, Rida Sulayman1, Muhammad Ahsan Kaleem2, Hafsa Shahzad1, Muhammad Summer1, Arooj Latif1, Tahreem Tanveer1

Selvaraj Thanga Malathi¹, Venkatraman Anuradha1*, Mohamed Yacoob Syed Ali2, Muhameed Sajjad Sarwar3, Nagarajan Yogananth2

...the water bodies, we can save the ecosystem.
...

Yoshio Hamano1*, Yasuji Kurimoto2 

...concentrations and monounsaturated fatty acid components. In post-mortem breast muscle, the effects observed in antemortem muscle disappeared. In contrast, AWP increased a component of polyunsaturated fatty acids in the breast meat. Therefore, this study suggested that growth and metabolic responses to LIP and AWP occur almost independently, and the results are different depending on feedi...

Akbar Hayat1*, Muhammad Asim1*, Tehseen Ashraf2, Ehsan-Ul-Haque1, Rabia Zulifqar2, Maryam Nasir3, Ahmed Raza1, Fahim Khadija1, Sohaib Afzal1 and Shafqat Ali1

...ia as T1 = Soil + silt + sand (2:1:1), T2 = Rice husk + sand + soil (2:1:1), T3 = Bagass + sand + soil (2:1:1), T4 = Wheat straw + sand + soil (2:1:1), T5 = Rice husk + sand + soil (1:1:2), T6 = Bagass + sand + soil (1:1:2) and T7 = Wheat straw + sa

Kazi Afsana Homayra Orchy1, Mst. Antora Akter1, Nelema Yesmin1, Md. Moshiur Rahman Khan1, Marzia Rahman2, Md. Mahmudul Alam1* 

... rabbits received normal saline topically. The wounds were evaluated for 45 days post-burning. Morphological features of wounds such as pain sensation, the development of crusts, exudates and pus, and morphometric assessment like wound contraction were recorded. On the 7th, 14th and 21st days post-burning, biopsies were taken for histopathological assessment. In this study, the morphological and morphometric data revealed th...

Walaa I. Mohameden1, Haidy G. Abdel-Rahman2, Ibrahim M. Hegab3* 

...ongside blood and plasma samples were harvested at days 0, 30, and 60 of the study for hematological and biochemical analysis. Results revealed an overall improvement in animal health, skin conditions, and liver and kidney functions. Significant (P < 0.05) increases in plasma proteins, zinc, and copper were recorded, while malondialdehyde, TNF-α, and MMP-9 significantly (P < 0.05) decreased. Furthermore, buffalo calves showed significant ( P < 0...

Karamat Shah1, Muhammad Fiaz Khan1, Zaib Ullah2*, Rashid Ali Khan1, Sajid Mahmood1, Naveed Akhtar2 and Muhammad Naeem Awan3

...ldlife that has an unpleasant outcome on both. Intimidation caused by carnivore towards injuries to individual, financial defense, damages to crops is increasing or the opinion that wildlife causes threats to human safety, health, food, and property. The current study was conducted from June to November, 2018, at Saiful Mulook National Park Khyber Pakhtunkhwa, Pakistan. For this purpose, 22 villages were selected in and arou...

Dajun Su1, Tao Deng2* and Mingshui Xie2

...ciferase reporter gene assay. CCK8 and flow cytometry were used to detect the proliferation and apoptosis of ovarian cancer cells in each group. Compared with normal ovarian epithelial cell FTE187, the expression of LOXL1-AS1 in ovarian cancer SKOV-3 cells was significantly up-regulated and the expression of miR-761 was significantly down-regulated; compared with the miR-NC group, the expression level of miR-761 in SKOV3 cells was significantly increased in th...

Lianci He1, Dingxi Bai2, Chenxi Wu2, Yizhu Zhong2, Shi Chen2, Xinru Bao2, Jing Gao2*, Chaoming Hou2*

...d CON group received the same volume of normal saline. All groups received treatment for 28 days. The rats’ body weight, fatigue time, activity and mobility, and mRNA and protein levels of interleukin-1 beta (IL-1β), tumour necrosis factor alpha (TNF-α), transforming growth factor β-activated kinase 1 (TAK1), TAB, IκB kinase (IKKα), IκBα, NF-κBp65 and cyclooxygenase-2 in th...

Maryam Beheshti1, Mohammad Reza Taheriyan2*, Sikander Shahzad3, Memoona Siddique4, He Tianwei5, Muhammad Farooq5*, Huzaifa Kashif6 and Naila Ilyas7*

...oybean, and palm-had the same refractive index (1.46) and showed no statistically significant different (P<0.05). Palm oil had a saponification index of 202 mg/g and a peroxide time of 5.8 hours compared to hemp and soybean oil. The greatest ionic index was found in hemp oil, which had a value of 136. In terms of oxidative resistance, soybean oil was more stable than palm and hemp oils, with a value of 5.25 hours. Linolei...

Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*

...ercentage range of eight samples was 91.79-92.37%. The filtration sequence was 44106250-54234844. Compared with the reference genome by TopHat software, the net reading ratio of the bovine reference gene at each stage was 93.17-9 4.23%, the ratio of sequence numbers to multiple sites of the genome was 2.99-4.89. The DEG was identified by using the fold change ≥2 and FDR <0.01 as cut-off values. There were 525 differentially expressed genes. GO and KEGG a...

Muhammad Ali1*, Rukhsana Perveen2, Khalil Ahmed3, Ghulam Raza1, Muhammad Akbar1, Maisoor Ahmed Nafees3 and Muhammad Ayub4

...r, Rodolia ruficollis Mulsant is treated taxonomically for the first time from Sindh Province of Pakistan. This may be a unique contribution to the genus Rodolia with the description of this species on the basis of important diagnostic characters including male and female genitalia. It is an important predator of scale insect pest, Icerya aegyptiaca (Douglas, 1890) which is dangerous insect pest upon different fruit trees including date palm, mango, mulberry a...

Qi Liu1,2, Weiyuan Li2,3, Qi Zhang2,3, Ziwei Wang2,3, Rui Gao1,2, Wenlei Liu1,2, Lei Zhang1,2, Ying Liu1,2, Tao Tian2,3 and Hongwei Yan2,3*

... years. A set of 13 microsatellite markers were used to evaluate genetic patterns across 2015, 2016 and 2019. We observed a significant Hardy-Weinberg disequilibrium across all stocks. The inbreeding coefficient (Fis) was positive (0.121–0.131) for all stocks. A loss of genetic diversity was detected in the 2019 stock and significant differences were observed for number of different alleles, number of effective alleles, allelic richness and unbiased expe...
Xiangjie Zhao1, Zheshi Kuang2, Jiyuan Yang2, Muhammad Bilal1 and Rongling Yang1*
...rol group was fed with basal diet containing 3% fishmeal, and the treatment group 1, 2, and 3 were fed basal diet with 30%, 60%, and 100% fishmeal substituted with fermented silkworm pupae (FSP), respectively. Broilers were fed the treatment diets in phase 1 (1-21 d) and phase 2 (22-42 d) during the experimental period. Results showed a non-significant difference (P > 0.05) between control and treatment groups on the aver...

Yingying Ye1, Chengrui Yan1, Kaida Xu2, Jiji Li1, Jing Miao1, Zhenming Lü1 and Baoying Guo1*

...ed primers for nine microsatellite loci resulting from an enrichment process based on the Wenzhou population. Eight out of nine microsatellite loci were used to analyze population genetic diversity of cuttlefish in China Sea. The results showed high levels of genetic diversity in all populations with highest mean number of alleles and mean effective alleles estimated in population Ningde and lowest in Qingdao. Heterozygosity...

Alisher Safarov1*, Nasreen Nasreen4, Firuza Akramova5, Shukhrat Djabbarov1, Adolat Mirzaeva5, Javokhir Esonboev5, Djalaliddin Azimov5, Mourad Ben Said2,3 

... with the genera Haemaphysalis (3 species) and Rhipicephalus (4 species) being the most dominant among the ixodid ticks. Insects comprised nine species from five families: Trichodectidae (1 species), Linognathidae (1 species), Pulicidae (4 species), Culicidae (2 species), and Hippoboscidae (1 species). The domestic dog exhibited the highest ectoparasite species diversity, with 21 identified species, while domestic and jungle cats harbored only four species eac...

Kinza Javed1, Muhammad Ali1, Waqas Farooq2, Majeeda Rasheed1*, Farkhanda Yasmin3, Samiya Rehman3, Sadaf ul Hira1 and Jawad Ali3

...ne. To control the dispersal of LSDV, infected animals should be quarantined, restricted its movement and place them in a place of no entry of vectors, arthropods. The recent review investigated new knowledge on several facets of the disorder, including its distribution, transmission, origin, pathophysiology, host vulnerability, diagnosis, preventiand therapeutic approaches.  Lumpy skin disease virus belongs to Poxviridae family and genus, Capripoxvirus. ...

Amirul Faiz Mohd Azmi1,4, Hafandi Ahmad1, Norhariani Mohd Nor1, Goh Yong Meng1, Mohd Zamri Saad2, Md Zuki Abu Bakar1, Norafizah Abdul Rahman5,6, Agung Irawan7,9, Anuraga Jayanegara8,9, Hasliza Abu Hassim1,3,9*

 

...ns when treated with the same dietary treatments, but Swamp buffalo were significantly (P < 0.05) higher in Ruminococcus albus and total fatty acid. This study showed that supplementing concentrates solely or a mixture with bypass fat into a grass-based diet could decrease methane production, as well as methanogens without giving a detrimental effect on rumen fermentation but also increase the degree of fatty acids satura...

Luthfiralda Sjahfirdi1*, Zulfi Atsiil Suandhy1, Tatang Mitra Setia2 

...nimal and all-occurrence sampling methods. Nest-building skills were assessed using the Likert scale. Moni (male orangutan) was always observed to nest using used nests. Meanwhile, Terra (female orangutan) was frequently observed building new nests, although she was caught sleeping on the forest floor several times. During observations, it was found that they prefer nest trees with a circumference of more than 1 m with a reasonably dense canopy refers preferre...

Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2* 

...the sperm. The optimal dosage for maintaining the integrity of the dog sperm’s membrane and enhancing its property parameters was determined to be a supplementation of 100 µg/ml essential oils, which demonstrated a significant difference from the control treatment throughout the 12-day storage period (P<0.05). In summary, the results indicate that the properties of dog sperm during preservation are influenced by the dosa...
Asadullah Memon1, Asghar Ali Kamboh1*, Saeed Ahmed Soomro2, Muhammad Ammar Khan3, Akeel Ahmed Memon4 and Hubdar Ali Kolachi1
...is in 178 sheep and goat samples obtained from Hyderabad, Tando Allahyar, and Mirpurkhas districts of Sindh Province of Pakistan. The results revealed that the seroprevalence of Q fever was significantly (p<0.05) higher than chlamydiosis and brucellosis (43.82% vs. 37.7% and 17.98%). The districts-wise incidences of seroprevalence were also significant in this study, as C. burnetii antibodies were significantly (p<0.05) higher in Hyderabad than Tando All...
Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
...is goal, 200 cattle milk samples were collected aseptically and screened for subclinical mastitis using the purposive sampling technique. Following Bergey’s manual of determinative bacteriology, S.aureus was identified and antibiotic susceptibility was tested using clinical laboratory and standard institute guidelines. On the other hand, resistant strains were put to antibacterial testing assa<...

Yao Xixi1, Zhou Rui2* and Ma Yinshan3

...ge, rumen fluid and milk samples were collected during green-up time, flowering time and withering time of forage to determine nutrient composition of forage, fermentation parameters of rumen fluid, and CLA in milk fat. The correlation analysis was studied in order to find out the influence of forage and rumen fluid parameters on the content of CLA in milk of grazing yak. The results showed that the phenological phases significantly affected the content of lau...

Jing Hu1,2,3 and Zhenhua Ma1,2,3*

...er aquaculture model (C: salinity 32.5%, ammonia nitrogen 0.01mg/L; T1: salinity 30.0%, ammonia nitrogen 0.04mg/L, T2: salinity 27.5%, ammonia nitrogen 0.10mg/L; T3: salinity 25.0%, ammonia nitrogen 0.18mg/L) to investigate the tissue damage of gills and head kidneys and the changes of serum indicators of hybrid grouper (Epinephelus lanceolatus ♂ &time...

Ghulam Sarwar Channa1*, Abdul Razak Mahar1, Inayatullah Rajpar2, Abdul Aziz Mirani3, Saleem Maseeh Bhatti2, Muneer Ali Bhagat1 and Wazir Ali Maitlo

...n: justify;">Rice (Oryza sativa L.) is the most important cereal crop in the world and it is used as a staple food for over one-third world’s population. Salinity is one of the major abiotic stresses, which is reducing the yield of rice crop in the world. The tolerance level of five native non-aromatic, coarse rice cultivars (IR-6, IR-8, DR-82, DR-83 and DR-92) was tested in the current study. A local nursery was established for each cultivar and healthy...

Rashid Saraz1, Saiqa Amur1, Zia-ul-Hassan1*, Naheed Akhter Talpur1, Inayatullah Rajpar1, Muhammad Sohail Memon2, Muhammad Nawaz Kandhro3, Khalid Hussain Talpur1 and Nizamuddin Depar4

...bility is highly indispensable to achieve the goals of agricultural sustainability and environmental health. Soil variability mapping of district Mirpurkhas, Sindh, Pakistan – globally known as the city of high-quality mangoes – was done for the first time. Surface (20 cm depth) soil samples were collected from 127 locations of six sub-divisions of district Mirpurkhas (locally known as talukas) i.e. Digri, Hus

Ni Putu Sarini1*, I Gde Suranjaya1, I Gusti Agung Arta Putra1, I Wayan Suarna1, Lindawati Doloksaribu1, I Ketut Puja2, I Ketut Gde Natakesuma3, I Gusti Ngurah Bagus Rai Mulyawan4 

...nt color patterns. Blood samples were taken from the 10 Bali cattle consisting of three having normal coat color patterns, three albino, two injin, and two poleng. The blood samples were extracted using a commercial kit and the MC1R gene was then amplified by using a forward primer 5’ AGT TGA GCA GGA CCC TGA GA 3’, and a reverse primer 5’ CCA GTC ACC ACA GAG CGT TA 3’. The PCR products were further...

Afifatul H.A. Adilah1, Junun Sartohadi2* and Suci Handayani1

...during rain events. Soil sampling under the plant canopy was used to measure texture, bulk density, particle density, porosity, macro pores, initial soil moisture, and organic matter. Coconut plants are considered better for the purpose of vegetative soil and water conservation than Mahogany. However, Mahogany must be arranged at the upper plot and Coconut at the lower plot to enhance runoff reduction. Coconut can reduce the volume of surface runoff significan...

Bushra Ainy Dars1*, Naeem Tariq Narejo1, Muhammad Hanif Chandio2, Hamida Narejo3, Majida Parveen Narejo4, Faheem Saddar5, Ghulam Dastagir6, Ghulam Abbas7 and Shahnaz Rashid7

...shoro. At 5cm intervals, samples of fish were separated into groups. Fish body dimensions, including fish head length, fin measurements and the diameter of fish eyes and mouth gape were examined. No significant difference between male and female was noted; though, there was a small variation in the percentages of lengths such as standard and anal fin, which were greater in females (85.0 and 15.3 percent, respectively) than in males (83.2 and 10.6 percent, resp...

Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3

...n was performed from the samples using a kit , and PCR amplification  was done using ITS4 and ITS1F  primers designed from the conserved regions as described in the previous studies. The amplified PCR products were sequenced from Macrogen Korea, via worldwide scientific Pakistan. Phylogenetic analysis of the obtained sequences was done based on the maximum similarity method using Mega 6.0. The spore was smaller as compared to Marasmiellus scandens (6...

Asim Zubair1*, Anam Farid Chishtti2, Zohaib Atta Mehdie3, Aamna Arshad3, Ghulam Akbar Malik4 and Iffat Batool5

...was employed to choose a sample of 100 livestock farm homes from the research area. To gauge the level of women’s participation, a composite score was created, and factors influencing women’s participation in cattle farming were identified using statistical methods. The findings show that women dominated practically all farming operations in the chosen area.

...

Sagir Hussain1, Erum Iqbal2*, Nasira Kazi2, Sher Wali Khan1, Qamar Abbas1 and Abdul Razaq1

...akistan. The analysis of samples yielded a new nematode species and two new reported species belonging to the order Tylenchida as new geographical records for Pakistan. Hemicycliophora pyri n. sp., is characterized by the broadly rounded lip region with two indistinct annuli, closely fitting sheath with 231-247 body annuli, stylet 95-100 µm long, non-raised and non-separated labial disc and gradually tapering long tail. Psilenchus vincigurrae Brzeski, 19...
Tehreem Fatima1, Jabbar Khan1,*, Hamid Shafiq2, Dost Muhammad3, Muhammad Rafi1 and Shoaib ur Rehman1
... conditions. Whole blood samples of 45 longevity individuals and 20 samples of control people were collected in D. I. Khan during September 2018 to October, 2019 after proper approval from Gomal University ethical review board and written consent of each individual was taken prior to collection of blood sample. For serum collection, blood samples were ce...

Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2* 

...sp;

...

Elihasridas, Mardiati Zain*, Roni Pazla, Simel Sowmen, Qurrata Aini 

...h supplement contains cassava leaves as a source of branched-chain amino acids, phosphorus, and zinc minerals, which was administered as follows: T0= ammoniated aromatic lemongrass waste, T1= T0 + 5% cassava leaf, T2= T1 +0.4% mineral phosphorus, T3= T1 +100 ppm mineral zinc, and T4= T1 + 0.4% mineral phosphorus and 100 ppm mineral zinc. The degradation of various components such as dry matter, organic matter, crude protein...

Uzma Ishaque1, Erum Iqbal1*, Shahnaz Dawar2 and Nasira Kazi1

...llected from rice (Oryza sativa L.) field in Nawabshah, Sindh, Pakistan, which has been described and illustrated. Mononchus oryzae n.sp., is characterized by its body, 1.508-1.528 mm long, lip region 23-24 µm wide, buccal cavity 34-36 x 16-17 µm with tooth apex located at 20-22% of stoma length and 3.9-4.2 anal body diameter long tail. M.oryzae n.sp. comes close to M. nudus Gagarin, 1991 and M. labiatus Shah and Hussa

Yu Liang Cao1, Yue Guo2,3*, Hui Zhang2 and Chun Sheng Wang2

...We concluded that 24,853 sales of P. canaliculata and A. fulica had been made through the Internet selling platform before 2019. Further surveys indicated that 51% sellers were located in endemic areas of Angiostrongylus cantonensis. However, no A. cantonensis-positive snails were found among Internet-sold snails. Laboratory animal infection experiments proved that all 4 breeds of snails could transmit A. cantonensis. Internet sa

Yingying Wang, Jianyi Yu and Lichen Zhou*

...e-linked immunosorbent assay to test for T. gondii antibodies. Antibodies (S/P>0.31) to T. gondii were found in 20 (26.32%) of the 76 tigers, while all blood samples tested through nested PCR were negative. This is the first investigation of T. gondii infection in South China tiger.

...
Tabark F Ahmed, Nibras Z. Yahya*
 
...fy E. coli O157; E. coli sample was taken from a hospital, the sample was cultured on MacConkey agar, Sorbitol MacConkey agar, chrome agar, eosin methylene blue agar, then VITEK® 2 System was performed Data showed the sample was positive as a result of E. coli O157. The second experiment examined the pharmacodynamics of ascorbic acid in various states, including alone and in combinatio...

Pham Tan Nha*, Le Thu Thuy

...rlic were added to the basal diet at 0.5%, 1%, and 1.5% (DM) concentrations. The trial lasted for 8 weeks, covering the period from 6 to 13 weeks of age for the Tre chickens. The findings demonstrated that supplementing the diet of Tre chickens with Garlic at a concentration of 1.0 - 1.5% (DM) resulted in weight gain and improved feed conversion ratio (FCR). Notably, the G1.5 treatments yielded the most favorable outcomes. In terms of Garlic type, the BG treat...

Deny Juniwati1, Hadri Latif2*, Trioso Purnawarman2, Zhang Shuqi3

 

...s. Nitrite levels in the samples were measured using a UV-Vis spectrophotometer. Utilizing an ultrasonic wave frequency of 40 kHz for 20 seconds could reduce nearly 80% of nitrite levels. It was considered more effective than standard methods, which were only able to reduce nitrite levels by up to 60%. 
 
Keywords | Edible bird nest, Nitrite, Ultrasonic wave
...

Satya Fahriani1, Hadri Latif2*, Herwin Pisestyani2, Zhang Shuqi3, Suyanto Chen3

...effect of retorts on the safety and quality of EBN. This study aimed to examine the nitrite level and rehydration capacity of EBN after undergoing a retort process. The samples were 24 raw uncleaned white EBN from 4 swiftlet farmhouses (SFHs) collected using purposive sampling. Two SFH from Sanggau District, West Kalimantan Province, Indonesia. Two SFH from Pekanbaru City, Riau, Indonesia....

Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein

...re examined and 332 milk samples were collected from (January – July) 2022. 10 ml of milk were collected in sterile test tubes, an equal volume of milk and CMT reagent were gently shaken in a paddle and the reaction was noticed within (10-15) seconds. The clinical form of mastitis accounted 9.35%, while the subclinical form accounted 52.4%, with positive (+ve) California mastitis test (CMT) results which are high significant. All milk

Novirman Jamarun1, Roni Pazla1*, Arief2, Elihasridas1, Gusri Yanti3, Rani Winardi Wulan Sari4, Zaitul Ikhlas4

...rnel cake, minerals, and salt. A randomized block design with four treatments and four groups of goat body weight as replicates was employed in the research. The four treatments were as follows: (T1 = mangrove leaves hay (MLH) 35% + fermented T. diversifolia (FTD) 5% + Concentrate (C) 60%, T2 = MLH 30% + FTD 10% + C 60%, T3= MLH 25% + FTD 15% + C 60%, T4= MLH 20% + FTD 20% + C 60%). Parameters measured included body weight gain, intake, and nutrient digestibil...

Khalid M. Al-Syaad1,2

...mg/kg/bw propolis. Blood samples were collected for biochemical analysis, and tissue samples (liver, kidney, and testes) were collected for histopathological examination. The results showed that the presence of CC increased (P<0.001) the levels of liver enzymes (ALT; alanine aminotransferase and AST; aspartate aminotransferase), creatinine and urea in the blood serum and lipid peroxidation product (TBARS). While decreased...

Rusul Adnan Dawood, Hasan F.K. Alghetaa*

...en HgCl2 with RES in the same doses mentioned earlier. All treatments lasted for four weeks. At the termination of the experiment, blood samples were collected to evaluate the renal function as well as to evaluate the redox system activity. Furthermore, peritoneal exudate was collected to determine the population of infiltrating cells by using trypan blue dye. Analyzed data revealed that there was renal dysfunction represent...

Abeedha Tu-Allah Khan and Abdul Rauf Shakoori*

... lacking. To address the said gap in knowledge, first we have identified the TFs specific to IL-1β a key player in mediating inflammatory process using online databases. Next, we identified the interacting partners of the significantly differentially expressed TFs out of the selected ones, and the mRNA expression changes were studied for both the differentially expressed TF along with its interacting partners. Finally, we explored the expression of these ...

Ramanathan Baluchamy* and Gnanamani Radhakrishnan

...ut millipedes). The soil samples from both sites were subjected for the analysis of biochemical compounds such as organic carbon, nitrogen, phosphorus, potassium and calcium. The site which is occupied by millipede (site I) showed remarkably high quantity of nitrogen (2.14%), phosphorus (23.10%), potassium (0.24%) and calcium (0.83%) than the site without millipedes (site II) during the month of November 2016. Similarly, an ideal C/N ratio (20.05) was observed...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...rains isolated from soil samples of Pakistan. Out of fourteen purified actinobacteria, antibiotic susceptibility profile confirmed five isolates showing resistance against tested antibiotics (ampicillin; lincomycin; rifampicin and erythromycin). Ribotyping confirmed that these isolates belong to Streptomyces species and were identified as S. monticola, S. septentrionalis, S. polaris, S. desertarenaee and S. lutosisoli. Primary screening of the five isolates us...

Hafiz Muhammad Arsalan1, Amina Arif1 and Muhammad Khalil Ahmad Khan2*

...rugs. Fresh venous blood sample (10 mL) of 170 CML diagnosed patients and 10 healthy individuals was collected in heparin vial from oncology department, Mayo hospital and Jinnah hospital Lahore, Pakistan. Biochemical profiling of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), nitric oxide (NO), advanced oxidation protein product (AOPP), advanced glycation end products (AGE’s) and micronutrients (retinol, ascorbic ac...

Yongman Guo1,2, Jundong Tian1,2,3, Dong Yang1,2 and Jiqi Lu1,2,3*

... in animals. Fresh fecal samples of adult Taihangshan macaques (Macaca mulatta tcheliensis) were collected during mating and non-mating periods, respectively, to understand the influences of mating status on physiological condition of wild macaques, through variation in fecal cortisol and IgA levels. The results showed that. (1) mean concentration of fecal cortisol in mating period (AM: 1751.12 ± 199.16 ng/g; AF: 1366.38 ± 117.85 ng/g) were signi...

Gang Liu*, Na Xu, Zhizhong Gong and Jiahui Feng

...its (OTUs) from 20 fecal samples (ten per lake). The OTUs from the Shengjin Lake geese represented 7 phyla, 27 classes, 81 orders, 151 families, and 395 genera; those from the Caizi Lake geese comprised 7 phyla, 28 classes, 73 orders, 133 families, and 232 genera. Ascomycota, Basidiomycota, Zygomycota and Rozellomycota were the dominant gut fungal phyla in the geese. The alpha-diversity indices differed significantly between the Shengjin Lake and Caizi Lake ge...

Kiran Afshan1*, Sobia Baseer1, Shanza Kiran1, Ghulam Narjis2 and Sabika Firasat1

...e-linked immunosorbent assay (ELISA) was performed to measure the IgG and IgM antibodies level. Univariate analysis and odds ratios were calculated to determine the strength of association. The overall T. gondii infection was 60% with LAT, 52% IgG, and 56.26% with IgM ELISA. The seroprevalence was 7.2%-15% among the fi...

Mohammad Bani Ismail

...Zizyphus jujuba var spinosa seeds contain SPN, a C-glycoside flavonoid used in traditional medicine. A growing body of evidence indicates they have pharmacological potential against a wide range of diseases. Spinosin (SPN), however, has not been examined in relation to doxorubicin (Dox) induced cardiac injuries. To find out if SPN can reverse Dox-induced cardiomyopathies, we conducted this study. Results showed that Dox treatment led to bigger cardiomyocytes, ...

Irtaza Hussain1, Muti ur Rehman Khan1*, Asim Aslam1, Masood Rabbani2 and Ahsan Anjum1

...e linked immunosorbent assay (ELISA). This technique is sensitive and cost-effective for detecting disease in large no of animals. For this purpose, serum samples from 350 goats and 91 sheep were collected on the 20th day post-infection for detection of antibodies, and essential information related to potential risk factors was collected through a questi...

Ijaz Ahmad1,5*, Haihong Hao1, Pascal Sanders2, Zafar Iqbal3, Saeed Ahmed4, Farhan Anwar Khan5, Zahir Shah5, Muhammad Ibrahim6 and Lingli Huang1

...justify;">The cefquinome samples from plasma were extracted and separated by HPLC on a reversed phase C-18 column with mobile phase of acetonitrile and formic acid (0.1%) solution- (90:10, v/v). The calibration curves were linear ranged from 0.01-5µg/mL. The limit of quantification (LOQ) and limit of detection (LOD) were 0.04 and 0.01 µg/mL. The recovery of cefquinome from plasma was 73.4%, 78.33%, and 77% for low, medium and high concentration

Feng-Yan Liu1,2,3, Ting Chen1,2,3, Yu-Hui Li1,2,3, Zheng Liu1,2,3, Jing-Jin Zheng1,2,3, You-Bang Li1,2,3* and Zhong-hao Huang1,2,3*

...ca mulatta) fecal pellet sampled from the Longhushan Nature Reserve, Southwest Guangxi, China. The results indicated that cortisol was negatively correlated with ambient temperature and sunlight, whereas T4 was positively correlated with sunlight. Cortisol levels sampled from spring/winter were higher than those sampled from summer/fall, while levels of GCs and T3/T4 were not significantly...
Nabila Rasheed1, Shumaila Usman2*, Madeeha Sadiq3, Bushra Wasim3, Kanwal Haneef4, Hafsa Shafqat3, Tabinda Urooj3 and Saman Rashid4
 
...issue of normal(healthy) samples and from the GDM affected sample. The isolated cells were characterized for the presence of stem cells markers by Immunocytochemistry. After characterization, the analysis of GDM effect on WJMSCS proliferation rate was analyzed by calculating the population doubling time of the cells at different passages (P0-P3). Moreover, the expression of the important c...

Sherein H. Mohamed1, Soad El Naggar2*, Ayman A. Hassan3, Mohamed A.M. Mousa3, Mohamed M. Basyony3, Mohamed F. Sadek3, Mohamed A. A. Ahmed4, Saadia M. Hashem5 

... diet group, C fed the basal diet and the other five groups (GE, ClE, ThE, TE, and CE) fed on basal diet supplemented with 200 ppm of garlic, clove, thyme, turmeric and cinnamon extracts respectively.  These extracts revealed an increase in final body weight, daily weight gain, digestibility coefficient of crude protein, crude fiber, neutral detergent fiber and acid detergent fiber, nitrogen balance, acetic acid and pro...

Mohammed Naji Odhah1,2, Faez Firdaus Abdullah Jesse1*, Bura Thlama Paul3,4, Bashiru Garba5, Zaid Mahmood1, Eric Lim Teik Chung6, Mohd Azmi Mohd Lila7  

...dotuberculosis is the causative agent of caseous lymphadenitis in small ruminants and is of considerable economic importance in many countries worldwide. The control of the disease in animals depends on the control of the disease in infected animals. Nevertheless, few countries where the disease was previously endemic have successfully eradicated the pathogen. This investigation was undertaken to determine the antibody titre (IgM and IgG) in does challenged wi...

Ishtiaq Ahmed1*, Hamid Ullah2, Zubair Ali2, Muhammad Sohail2, Yasir Amin2 and Afrasyab1

...district Haripur. Faecal samples (n=633) were randomly collected and were examined for the presence of helminths parasites, in which 463 (73.0%) were found positive for the eggs of different GIT helminths. Seven species of parasites including: Eimeria Spp, Ascaris spp., Strongyloid spp., Fasciola spp. Trichuris spp. Monezia spp. and Schistoma bovis. as well as mixed species were identified as per their intensity. It was noted that prevalence was highest in goa...

Sajid Ali1, Ghulam Abbas1, Shahnaz Rashid1, Asma Fatima1*, Abdul Malik1, Dilawer Ali1, Muneer Hussain Bijoro1, Ushra Batool Hashmi1, Rumaisa Abdul Rahim1, Shahid Hussain2, Kashif Ali3, Jabbar Memon4 and Javeria Khourshid1

Asif Sadam1*, Rahmat Ullah Khan2, Karim Gabol2*, Muhammad Awais3,4, Mohammad Ismail1, Sajid Mahmood1, Muhammad Aslam4 and Hamidullah5

...el information on the causal factors that lead to hunting and illegal trade of waterhen Amaurornis phoenicurus in a remote villages of district Mardan, Khyber Pakhtunkhwa, Pakistan. Data were collected by adopting a mix scientific approach including Participants’ observations, interviews and structured questionnaire. Our results showed that the local villagers of the remote area of district Mardan were the active observers of trapping, over hunting and t...

Nasratullah Baloch1, Naeem Tariq Narejo2, Hamida Narejo3, Muhammad Farooq Hassan4, Ghulam Abbas5, Shahnaz Rashid5, Muhammad Hanif Chandio6, Faheem Saddar7 and Majida Parveen Narejo8

Jamshid Ali1,2, Sabeeqa Usman Malik1*, Muhammad Irfan Ashraf1, Jia Zhongkui2, Zuhair Husnain3 and Saeed Gulzar1 

... (Pakistan). Twenty-five sample plots were marked in the study area to collect tree diameter, height and density-related information. Allometric equations were used to estimate total carbon stock from field data. The results depicted that the mean total biomass in the study area was 54.90 (t ha-1) with the above-ground biomass (AGB) 43.60 t/ha and below-ground biomass (BGB) 11.34 t/ha.. The mean carbon dioxide equivalents (CO2e) were 94.50 t/ha. The result of ...

Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3

...e collected using a disposable syringe and centrifuged, then supernatant in the collection, stored and frozen with -20 oC and growth factor concentrations were measured using the ELISA method. The results showed that there were 228 developing embryos (41%) after 48-hour of culture with 2-, 4-, 8-, and 16-cell division. EGF concentrations in the medium of 2-cell to 16-cell ranged from 0.853...

Mudawamah Mudawamah1*, Sumartono Sumartono1, G. Ciptadi2, E. Susanto3, Y. Hartoyo4, L. Affandhy5,6

...d descriptive with total sample of 60 heads (morphometry and physiological status) and 24 heads (total protein).  There are three breeds of sheep: Sapudi, Dormas (Dormer x Sapudi), and Suffas (Suffolk x Sapudi). Sapudi, Dormas, and Suffas Ewes with only one child each lambing were abbreviated as SAS, DOS, and SUS. The ewe has twin lambs designated by

Reza Esmaealzade Dizaji1, Arasb Dabbagh Moghaddam1*, Arash Ghalyanchi Langeroudi2, Mohamad Foad Heydari3, Seyyed Javad Hosseini Shokouh4 and Ana Shirzad Shahrivar5

...rrent investigation. The same results were corroborated by the pathological score of skin wounds stained with H&E. Researchers found that in comparison to the control group, those in both the PRP/BNF and PRP groups had significantly lower expression levels of the mRNAs for interleukin 1, interleukin 6, and interleukin 10 than those in the untreated group. In contrast, those in the untreated group had significantly higher expression levels of those genes. T...

Jaiperkash Moolchandani1*, Geeta Moolchandani2, Syed Mohsin Turab3, Nasima Iqbal4, Ambreen Rehman5 and Shaima Sultana Memon6

...hile depression remained same with the decreasing duration of the disease but the results were non-significant. Lesser the mini mental status examination (MMSE) score, higher the frequency of depression, anxiety and insomnia. It is concluded that the non-motor symptoms are quite common among PD patients and out of all, the most common one is depression. Frequency of non-motor symptoms varies within different age groups as well as the gender and duration of dis...

Bushra Allah Rakha1*, Farah Qayyum1, Muhammad Sajjad Ansari2, Ali Akhter1, Komal Shakeel1, Javeria Batool1, Faryal Akhtar1 and Sehrish Hina1

...uction was garanda (Carrisa opaca; 58.7%) followed by sanatha (Dodonaea viscosa; 26.25%), panch phuli (Lantana camera; 6.25%), beri (Zizyphus mauritiana; 3.75%), kronda (Carissa macrocarpa; 2.5%) and mallah (Zizyphus nummularia; 2.5%). Clutch size in white-cheeked bulbul ranged from 1 to 5 eggs with mean clutch size of 2.6 eggs. Maximum percentage of nes...

Arshad Khan, Mohammad Ihsan, Maryam Bibi, Gul Rahim, Fazl Ullah, Komail Khan and Ali Hazrat*

...eat posed by rising soil salinity to agricultural production is serious. The Harmonized World Soil Database is the only database that currently offers data on worldwide soil salinity; however, it has a number of limitations regarding the way soil salinity is measured. A new assessment is therefore required. The present research work was conducted at Botanical Garden, Department of Botany, ...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

...nisms isolated from fish samples. A total of 120 fish samples were collected from fish retail market and ponds (60 from each source) comprising of three fish species i.e., Labeo rohita (LR; local name Rohu), Catla catla (CC; local name Thaila) and Cirrhinus mrigala (CM; local name Morakhi). The samples were processed for isolation of bacterial organisms using conventional culture method an...

Peter Amuguni Mbato*, Wanzila Usyu Mutinda, Noah Kipkoech Maritim, Nicholas Edwin Odongo 

...nd 8 weeks of age. Liver samples were collected at 8 weeks of age and processed for culture and sensitivity. Relative risk analysis (RR) was used to determine the association between confinement as a risk factor and bacterial infection. Birds in the FRPS had lower body weights that probably results less pressure on their skeleton. Treatment D birds had severe foot pad lesions (P < 0.05) compared to the other treatments at weeks 6, 7 and 8 and the severity d...

Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2

...e II NDV vaccines at the same days, respectively. Furthermore, groups C and D act as positive and negative non-vaccinated controls. At 30 days of age groups A, B and C were challenged with VNDV genotype VII1.1 isolate, where the clinical manifestations, gross post-mortem lesions, Humoral immune response and also quantification of virus shed post-challenge (PC) via real-time QRT-PCR were all screened and precisely recorded. The results revealed that, group A ch...

Elfadil, G.E.1,3, G.F. Gouda1*, M.A. Rashed2, A.R. Shemeis1

...ctively. Restricting the same index (I1) to result in zero genetic changes in both of HLP and M:B ratio, simultaneously (I1(HLP, M:B)), is expected to reduce the RTI by 20%. It could be recommended that in case of sub-optimal levels of HLP and M:F ratio, the use of the best reduced index (I3) is expected to develop NZW rabbits with more efficient capacity in meat production, while the use of the double restricted form of the full index I1(HLP, M:B) is recommen...

Eman M. Abdelhady1, Nader Y. Mostafa1, Mohammed A. El-Magd2* and Ghada A. Kirrella1

...mpare the effect of chitosan powder (CP) and nanoparticles (CNPs) on the microbiological quality and shelf life of beef minced meat   during its refrigerated storage for 9 days. The size of CNPs ranged from 150 to 350 nm as determined by transmission electron microscopy. Comparing the effect of the two chitosan products, CNPs exhibited higher antibacterial properties against S. aureus and E. coli, as revealed by lo...

Tahir Rasool Qamar1, Sanaullah Iqbal1*, Muhammad Nasir1 and Habib-ur- Rehman2

...l group receiving only basal diet while G1 was normal control group. The amount of chicory roots powder given was 5% w/w mixed in basal diet. It was observed that group G4 showed better results overall. Enzymatic activities of pathogenic bacteria were lowest in this group and were significant (p≤0.05) when compared to group G2. Percentage of total aberrant crypt foci (ACF) inhibition was also higher (30.14%) in group G4 a...

Xu Yun-Ming1*, Sun Zhi-Yuan1, Yang Jian-Bo1, Bian Rong-Rong2, Ren Hong-Lin3, Zhong Si-Yuan1, Cai Yu-Hong1, Peng Jing1 and Bao Hua-Xia1

...othermal amplification assay has been developed to detect it in uniformly items. According to the entFM gene of B. cereus, four primers were designed, and reaction components and conditions optimized. Sensitivity was compared between optimized LAMP assay and the conventional PCR. The optimal reaction conditions of LAMP assay (25uL each reaction) comprised 1.0 mol/L betaine, 6 m mol/L Mg2+,...

Peng Chen1, Jiaqi Li1, Hongbo Li2, Qin Lu3, Wei Liu1,4,5* and Jianliang Zhang1*

...enome were basically the same as those of Anseriformes species. The mean base composition of the mitogenome of Anseriformes was T (22.31 ± 0.51%), C (32.63 ± 0.64%), A (29.36 ± 0.64%), and G (15.71 ± 0.52%), indicating a slight specific bias towards A and C. AT content ranged of the mitogenome was from 50.27% to 55.31%, with an average value of 51.67 ± 1.10%, higher than the GC content and similar to that of birds in general ...
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...: normal;">Infectious bursal disease virus (IBDV) is a highly contagious disease of young chickens, that results in bursal necrosis and severe damage of the immune system. IBDV remarkably persist to affect the poultry industry in Egypt. Hence this study was done to identify and characterize very virulent Infectious Bursal Disease virus in Egypt during the period 2019-2020. bur

Wei Wang1, Meifeng Zhou2, Yuebo Liang1, Fan Zhang1, Zhong Wu3, Shaowei Mo4* and Yi Qing Wang1*

...s determined using MTT assay. Cell apoptosis was analyzed by flow cytometry. Protein and mRNA expression was measured using western blotting and RT-qPCR, respectively. The mRNA and protein level of YAP was significantly increased in the tumor tissues of HER2-positive breast cancer patients. Consistently, the expression of YAP and TAZ were both dramatically upregulated in SK-BR-3-TR cells. The cell viability was increased, while cell apoptosis was inhibited in ...

Tasleem Akhtar1*, Muhammad Farooq Nasir2, Imran Bodlah2 and Muhammad Adnan Bodlah3

...f hyperparasitoids viz. Asaphes suspensus (68%) and Pachyneuron aphidis (54%) were involved. Examination for phenotypic polymorphism showed that field population of A. smithi contained both dark and light pigmentation pattern of abdominal segments while laboratory reared samples were only dark pigmented in both sexes. The findings of this study can help in defining strategies for the rearing to release this parasitoid in bio...

Nwafili Sylvanus Anene, Ibinabo Jessica

...iotics and self-prepared saline water. The study also show that specialized equipment were not used in transportation and palm oil and some chemicals were added to transportation medium as anti-stress. There is the need to train catfish farmers’ on biosecurity measures and record keeping to further drive the growth of aquaculture production in Nigeria.


 

Yuyu Wang1,2, Mingzhu Li 3,4, Gang Lin4, Xiaohua Guo5, Aihua Sun5, Hao Dong5, Qinghui Ai1,* and Kangsen Mai1,*

...significantly higher docosahexaenoic (DHA, 22:6n-3), n-3 PUFAs content and n-3/n-6 ratio in muscle than fish fed diets S0 and S15 (P<0.05). Fish fed diets S5, S10 and S15 had significant lower C18:3n-3, eicosapentaenoic (EPA, 20:5n-3), C18:2n-6, n-6 polyunsaturated fatty acids (PUFAs) content in muscle than fish fed the control diets (P<0.05). No significant differences were observed...

Yang Pan1,2, Yang Huang3, Guo-hai Wang4* and Qi-hai Zhou3*

...tant roles in seed dispersal. In this study, we assessed the contributions of bird functional traits (behavioral traits: foraging quantity, number of birds per visit, and foraging duration; morphological traits: weight and body, wing, and tail lengths) to both seed removal patterns and dispersal distances of Cayratia japonica in an urban garden in southwest China. Eleven bird species were recorded feeding on its seeds, and e...

Muhammad Hussain1, Yasir Ali2*, Baber Iqbal1, Muhammad Azhar Iqbal1, Salman Ahmad3, Muhammad Zeeshan Majeed4, Hafiz Muhammad Aatif2, Muhammad Saeed1 and Muhammad Jawad Yousaf1

...re Research Institute Faisalabad, during 3rd week of November 2019-2020 and 2020-2021 crop seasons. To monitor the changes of rust virulence pattern in the region, a set of rust trap nurseries consisting of 21 stripe (Yr) and 36 leaf rust (Lr) lines were grown in the Research Area of Plant Pathology Research Institute during the same cropping seasons. A total of 11 Genotypes viz., Ass-11, V-13266, V-11C023, V-12266, V-08203,...

Kandiah Pakeerathan*, Konesalingam Jeyavithuyan, Aruchchunan Nirosha and Gunasingham Mikunthan

...anana and maize leaves), sawdust, and kitchen waste were used as mushroom substrate and then mushroom grown waste was converted into bio-fortified vermicompost using exotic earthworm Eisenia foetida+ Trichoderma viride + Pseudomonas fluorescens. Onion growth parameters, yield, and DSI were recorded from the experiment conducted in a complete randomized design and subjected to ANOVA using SA
Yousef Ahmed Alkhamis1,2*, Basanti Mondal3, Roshmon Thomas Mathew2, Ganesan Nagarajan4,5,6, Sheikh Mustafizur Rahman3, Md. Mostafizur Rahman7, Adnan Alhajji1 and Md. Moshiur Rahman3,8*
...in coastal regions where salinity changes happen regularly because of different natural calamities. This study was conducted to explore the effects of periodic changes of salinity on different phenotypic traits in Nile tilapia (Oreochromis niloticus) in order to find out any traits that can be expressed through compensatory growth. Equal sized juvenile Nile tilapias were randomly assigned ...

Nasir Ali1,2,6, Muhammad Ilyas2,3, Nazia Akbar1,2*, Gohar Rahman2, Shakirullah Khan4, Mujaddad Ur Rehman6, Abdul Samad5, Habib Ahmad1,2 and Bibi Nazia Murtaza7*

...his DNA can be used as a sample to study different parts of a population that lived a long time ago. In order to study the continuity in maternal lineage between ancient and modern people of the north-western province, polymorphisms in the HVSI region of mitochondrial DNA sequences of 56 ancient samples (1000-3000 old) and 303 modern samples from three different regions (Chitral, Swat and ...

Sajida Batool, Iqra Ashraf, Shafaat Yar Khan, Sitara Shameem* and Breha Kazmi

...e-linked immunosorbent assay (ELISA). C. procera treatment exhibited a significant decline in pro-inflammatory cytokines and an increase in anti-inflammatory cytokines on PHA stimulated T-cells in time-dependent and dose-dependent manner. These findings are suggesting the immunomodulatory potential of methanolic leaf extract obtained from C. procera that together with its other therapeutic...

Caiyun Li1, Tao Xu2, Luyang Zhang3, Xuesong Yan4, Yu Xin5, Li Li6 and Jinghua Chen7*

...red the advantages and disadvantages of feeding forage fish and compound feed for snakehead culture. In addition, the development status and trend of compound feed for snakehead were analyzed. This review will provide insights into the green aquaculture development and industrial upgrading of snakehead in Shandong, China.

...

Jialing Zhang1, Yan Ma2, Lili Lian3 and Min Wei1*

...ng while maintaining the same long-term effectiveness in avoiding endometriosis recurrence and benefits for fertility outcomes.

...

Asifa Majeed*, Amir Rashid, Palvasha Waheed, Asma Faryal, Aden Razaq and Ayesha Maryam

Fahad A. Al-Abbasi1*, Abdullah Samer Habib1, Vikas Kumar2 and Firoz Anwar1

...tion workers. Sufficient samples (approx. 422) from imported fish and chicken were randomly collected and analyzed for total plate count (TPC), Staphylococcus aureus, Vibrio parahaemolyticus and Pseudomonas. Five healthy workers directly involved with processing, handling from various departments without any prior disease were selected for blood profiling. Significant increase in S. aureus colonies were observed in fish samp...

Samia Afzal1*, Jahanzaib Ahmad1, Iram Amin1, Liaqat Ali2, Muhammad Shahid1, Mohsin Ahmad Khan1 and  Muhammad Idrees1

...s Delta Virus (HDV) is a satellite virus of hepatitis B virus (HBV) as HDV relies on hepatitis B surface antigen (HBsAg) for viral development and release from hepatocytes. It causes the most severe viral hepatitis and suppresses the serological and molecular markers of HBV infections; for example, Hepatitis B envelope antigen (HBeAg), inhibition and reduction of HBV DNA titer during HDV infections. Very few studies described the clampdown of HBV-related marke...

Sumaira Kousar* and Safdar Ali Shirazi

...loying the Revised Universal Soil Loss Equation (RUSLE) model alongside cutting-edge Remote Sensing (RS) and Geographic Information System (GIS) techniques. The RUSLE model, celebrated for its simplicity and efficiency, offers a lens to assess soil losses quantitatively, categorizing the basin area into five distinct vulnerability groups predicated on their proneness to soil erosion. The study’s findings spotlight alarming terrain vulnerability, specific...

Mohammed A. El-Sayed1*, Mahmoud H Hatab2, Heba AEM Assi3, Nashaat S Ibrahim2, Hisham M Saleh2, Waheed AA Sayed2, Birgit A Rumpold4 

...r organs, spleen, and bursa of Fabricius of quails when compared to diet A. The LBW, relative carcass weight, and measured organs of the diet A group were significantly impacted by the interaction of dietary treatment and HS, whereas these traits had no or minimal effects on the diet B and diet C groups. The HSP70 gene transcription level was highest in birds exposed to cyclic HS and fed control diet A, and lowest in birds in the diet C groups. Conclusively, 1...

Amistu KU1,2*, Monahan FJ2, Wims P2, Yonatan KY1 , Fahey AG2, Takele TA3 

...op residues (14.7%) as basal diets, whereas for the rainy season fatteners almost fully depend on natural pasture (97.7%). However, fatteners use corn/fresh maize (87.7%), coffee leaf boiled together with water (44.4%), haricot bean (40.5%), cassava root (28.8%), mixed concentrates (21.6%), enset root (14.7%), and local brewery by-product (9.8%) as supplementary feed. The mean age of cattle when fattening began is 5.32&plusm...

Javed Hussain Umrani1, Roseena2, Ashfaq Ahmed3, Saba Iqbal2, Amtul Sami4, Mumtaz Ali Salito5 and Gulnaz Parveen2*

...gn: justify;">Studies on salinity tolerance at early seedling stage of wheat against various salinity levels were carried out under controlled conditions. Five wheat varieties (TD1, TJ-83, Moomal, Sarsabz, Kiran-95) were obtained from different Agricultural Research Institutes of Sindh. The research was conducted in the Department of Crop Physiology, Faculty of Crop Production, Sindh Agric...

Tahir Abbas Khan1*, Imran Ashraf1*, Athar Mahmood1, Muhammad Ilyas2, Sardar Alam Cheema1, Muhammad Mahmood Iqbal3 and Muhammad Umair Hassan1

... imperious nutrient necessary for plants growth and development. Similarly, cultivars also differed significantly in terms of growth, yield and P utilization. Therefore, present study was performed to assess P efficient maize genotypes on the basis of growth and yield. The study was comprised different maize genotypes; CS-2Y10, KSC-SB 9663, FH-949, 30Y87, NT-6621 and DK-6789 and of diverse P levels; control (No P), 40 and 80 kg P ha-1. The maximum root length ...

Arnold Christian Tabun*, Ferdinan Suharjono Suek, Cardial L Leo Penu, Johanis A. Jermias, Thomas Lapenangga  

Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*

...roblem due to inadequate safety measures. A severe burn is associated with release of inflammatory mediators including reactive oxygen and nitrogen species (ROS, RNS), which are implicated in pathophysiological events in burn patients. Excessive productions of free radicals are harmful and implicated in tissue damage and multiple organ failure. Supplementation of antioxidants has proven beneficial in decreasing free radicals in burns. The aim of the present st...
Sami Ullah1, Choudary Ihtasham Ali1, Umar Ijaz Ahmed1*, Shoaib Nasir1 and Amjad Masood2
...r vanaspati ghee. At the same time, the taste has a negative and significant influence on the choice of vegetable oil. The estimates indicate that the highest income group in the data prefers edible oil more than the lowest income group. As vegetable oil is costly as compared to ghee, consuming healthier food, price is an essential criterion for low-income consumers. Based on the findings, the availability of good quality vegetable oil to the masses requires t...

Nadeem Akmal1, Abid Hussain1*, Muhammad Yousaf2, Waqar Akhtar1 and Hassnain Shah1

...ion are more or less the same across both methods. Thus, it is reaffirmed that mechanical transplanting of rice (MTR) is labour saving, and more productive than conventional sowing method. Productivity of Basmati-Super and Basmati-386 rice crop palnted through MTR at sample farms were higher than conventionally transplanted crops by 12.23 and 10.56 percent, respectively. In case of Basmati...

Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1 

...e pursuit of alternative safe measures has been paramount. Among the prominent alternatives, biological control has garnered attention, wherein environmentally safe organisms such as nematodes and fungi are employed. This study examines their efficacy in controlling the insect larvae and pupae. The integration of these indigenous biological control agents yielded notable mortality rates for the targeted larvae and pupae. The...

Frah Razzaq Kbyeh1, Ahmed N. Abedsalih2* 

...57:H7 two hundred urine samples were collected from adult women patients suffering from UTIs during a period from February 2022 to July 2022 in Diwaniyah city Iraq . All urine samples were incubated aerobically at 37°C for 24-48 hrs on blood agar, MacConkey agar as well as biochemical tests identified special media and the isolates. The isolates were confirmed and diagnosed by Vitek assa<...

Fatima Ali1*, Rimsha Tahir1 and Tahir Rehman Samiullah2

...ressing for the last thousands of years, however its effectiveness in clinical trials not yet been fully recognized. It is now known that honey acts as a biologic wound dressing with multiple bioactivities. Major aim of this study was to evaluate the efficacy of honey in combination with N-acetyl cysteine (NAC) for the treatment of excisional wounds in rats. Sprague Dawley (SD) rats were inflicted with excisional wounds. Wounds were treated with honey as contr...

Meryem Betmezoğlu1*, Dilek Arsoy2 and Mahmut Çerkez Ergören3

...fare, together with food safety and security. Goat production and indigenous goat breeds (Cyprus Native hair Goat, Damascus) are important for the Cyprus’s agricultural diversity Therefore, the allele frequencies of Caprine Prnp gene variants should be well understood for goat breeding programs in Northern Cyprus. This current study was designed to determine the putative allele frequencies and genotype distributions of Caprine­ Prnp gene variations i...

Mamona Jabeen1, Sumra Naz1, Hina Amjad1, Khalid Abbas1*, Sajid Abdullah1, Muhammad Anjum Zia2, Taqwa Safdar1 and Muhammad Sarfraz Ahmed1

...udied by using five microsatellite loci in a total of 150 individuals. The C. marulius population exhibited a moderate level of heterozygosity. The mean value of observed heterozygosity ranged between 0.700 – 0.833 while the expected heterozygosity varied between 0.863 – 0.868. Significant deviation from HWE (P<0.05) was observed in all the populations due to the deficits of heterozygotes suggesting either due to inbreeding or recent mixing of s...

Mahmoud Abdelhady Metwally1*, Mona Abdelftah Ali1, Farouk Abdelmohdy1, Mohamed Kassab1, Tarek Kamal Abouzed2 and Khalil Fathy Abou-Easa1

...enyl-tetrazolium (MTT) assay showed decreased proliferation, while flowcytometry showed increased apoptosis and cell cycle arrest in the G0/G1 phase with inhibition of entry into the S phase. RT-PCR showed p53 upregulation and Bcl-2 downregulation in mRNA expression. Moreover, Western blotting and flowcytometry revealed elevated p53 and lowered Bcl-2 protein levels. In addition, ELISA find...
Yongming Zhang1,2, Yuyu Wang2*, Jing Jia2, Sihe Zhang2, Xiaohua Guo3, Aihua Sun3, Hao Dong3, Peiqiang He4 and Xiefa Song1*
...r (P>0.05). n-3 polyunsaturated fatty acids (PUFAs), n-6 PUFAs and total PUFAs content were lower in wild than that in farmed Conger myriaster (P<0.05). Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were higher in farmed than wild Conger myriaster (P<0.05). Farmed fish showed significant high content in K and Mg but low in Hg, As and Pb compared to wild fish (P<0.0...

Muhammad Nadeem1, Jamshaid Iqbal2, Tariq Mustafa3, Gul Rehman2, Muhammad Faisal Shahzad2, Muhammad Younas4,5*, Aftab Ahmad Khan6, Ameer Hamza2, Abdul Ghaffar1 and Muneer Abbas1

...i (Zimm) (Mealikil-VL), Isaria fumosorosea (Wise) and Beauveria bassiana (Bals) (Racer-BB) were investigated against the mung bean thrips, Megalurothrips distalis Karny (Thysanoptera: Thripidae). Evaluations were based on thrips population, percentage reduction in number of thrips per flower, and percentage damage of the mung bean pods. On an accumulative basis, B. bassiana at 7.5 % concentration resulted in the reduction of...

Rahamdad Khan1, Saad Muhammad2, Muhammad Haroon3, Saad Jan1 and Syed Majid Rasheed1*

...sterophorus and Cannabis sativa. The results showed that under reduced light duration (2 hours), plant growth performance (i.e., biomass, plant height, leaf area, and number of branches) was all reduced. In addition, with increasing light duration (9 hours), both species grew faster and recorded their maximum biomass plant height, leaf area, and number of branches. Both weed species grown under reduced light duration could not reach maturity and complete their...

Momina Raheem1, Muhammad Fiaz2*, Muhammad Mushtaq1, Umana Niazi1, Evelyn Saba3 and Mansoor Abdullah3

Haiyan Nan, Ran Feng, Guiling Zhang and Jingqin Liu*

...In the left and right dorsal foot arteries, the inner diameter and blood flow of the T2DM group were lower than those of the control group (P<0.05). The peak flow velocity and spectral width of the T2DM group were higher than those of the control group (P<0.05). Compared with the control group, the FGF23 protein content and the F-IMT thickness in the T2DM group were increased (P<0.05). Compared with the control group, the proportion of type I, type II...

Saima Naz1, Ahmad Manan Mustafa Chatha2, Rifat Ullah Khan3*, Saba Iqbal1, Nimra Amjad1, Azka Kiran1, Ammara Javed1, Maria Lateef1 and Amna Nawaz1

...lpur, Pakistan. All fish samples were collected from three different sites (Panjnad, Abasia, Abu Dhabi canal) during February 2020 to January 2021 using different fish inclusive. The evident fish groups found were Cyprinids, Catfishes and others including (Channa punctata, Oreochromis niloticus, Oreochromis mossambicus, Bagarius bagarius). The data was statistically analyzed using ANOVA. Further relative abundance (Panjnad c...

Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik

...antibodies. Clot lysis assay was used to estimate the biological activity of HSA-SK. For half-life estimation, proteolytic and thermal stability of HSA-SK was also checked.

...

Bayanda Mdoda*, Faith Machabi, Busisani Wiseman Lembede, Eliton Chivandi 

...ce, feed intake and utilisation efficiency, gastrointestinal tract (GIT) organ, and GIT accessory organ macromorphometry, visceral fat mass and carcass yield. One hundred and twenty unsexed 1-day-old Cobb 500 broiler chicks (10 chicks per replicate with 3 replicates per diet) were randomly assigned to four dietary treatments where zingerone replaced zinc bacitracin (ZnBcn) at 0 mg/kg (control: 500 mg/kg of zinc bacitracin), 40 mg/kg; 80 mg/kg and 120 mg/kg in ...

Ayoola J. Shoyombo1, Ake A. Moses1, Comfort I. Ukim2, Mustapha A. Popoola2, Olayinka O. Alabi1, Noah C. Edozie1, Faith Ogbor1, Jacob Kuusu1, Ekemini M. Okon3* 

...animals were kept in the same environment. The results showed that most hormones in the does were nominally superior during the wet season compared to the dry season, while progesterone and luteinizing hormones were significantly (p<0.05) higher during the wet season. Only testosterone was significantly (p<0.05) higher in the wet season compared to the dry season in WAD bucks. All other measured hormones in WAD bucks showed no clear pattern. This study h...

Syed Nouman Shah1, Anees Ur Rahman1, Abdul Kabir1,2*, Midrar Ullah3, Shahab Ahmad Nawaz1, Muhammad Said1, Abdul Hafeez Bukero1, Maaz Ahmad1 and Muhammad Sadiq1

...rage conditions and feed samples; well-mixed feed formulations with mycotoxin binders; diversified feed ingredients and optimized feed processing techniques can reduce mycotoxin levels in silage and their negative impacts on livestock. We conclude that a comprehensive understanding of mycotoxins in silage and the implementation of effective management strategies are essential for sustainable livestock production. Future research should focus on developing alte...

Abnet Mekonnen1*, Kadir Abderehman1 and Feti Seyaka2

..., duration of action and safety of a specific drug in a given clinical situation. A local anaesthetic disrupts ion channel function within the neuron cell membrane preventing the transmission of the neuronal action potential while general anesthetics like ketamine disrupt pathways within the cerebrum and stimulates the reticular activating center. Local anaesthetics affect all excitable tissues in the body, so toxicity can occur when sufficient amounts of the ...

Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A

...in chickens and AI appraisal of poultry farmers’ knowledge, attitude and practice in Abuja Municipal Area Council, Nigeria. One hundred (100) blood samples collected randomly post slaughter from chickens in live bird markets were screened and characterized using Agar Gel Immuno Diffusion and Haemagglutination Inhibition Tests at the Avian Influenza laboratory, of the National Veterinary Research Institute, Vom Plateau ...

Khwaja Junaid and Shah Alam Khan

...ding pest of rice, Oryza sativa L. (Poaceae), and is widely distributed throughout the rice producing countries in Asian, causing considerable yield losses. Cultivation of susceptible rice varieties is among the major reasons of insect pest outbreak. Contrary, growing resistant rice varieties plays a key role in keeping the insect pest population below economic threshold level. Growing resistant varieties is considered as sa...
Akram Ali Baloch1*, Adeel Ahmad2, Kaleem U. Kakar3, Sara Naudhani1, Samiullah Khan1, Agha Muhammad Raza3, Imrana Niaz Sultan1, Humaira Zahid4, Saadullah3 and Shakeela Daud1*
...tween 10-24 years. Blood samples were collected and DNA extraction was performed by inorganic method. DNA was amplified by polymerase chain reaction and subsequently sequenced to confirm any genetic variability in the affected individuals of the families. As a result, two different missense mutations (c.830C>A resulting p.Ala277Glu and c.332C>T resulting p.Pro111Leu) in affected individuals in two of the families were identified. The nonexistence of muta...

Fatimah A. Al-Saeed 

...that orally administered saline solution. The second group was exposed to Cd in a dose of 15 mg/kg b.w. The third group was treated with olive leaves extract (600 mg/kg b.w.) and exposed to Cd. The fourth group was treated with olive leaves extract (600 mg/kg b.w.) only. At the end of the experimental period, blood samples were collected and analyzed. The obtained results showed that the levels of selected blood biochemical ...

Nazia Haider, Asia Bibi* and Nahid Kausar

...tionnaire at the time of sampling after obtaining informed consent. ABO and Rh blood group was identified by using Antisera (including Anti-A, Anti-B and Anti-D). The analysis of haematological indices was done by using automated haematological analyzer (Sysmex Co. Japan). The mean maternal age in this study was 30.35±7.94 years for cases and 28.53±6.59 years for controls. Highest frequency of miscarriages 90 (30%) was observed in cases of age gr...
Rabail Hassan Toor1,3, Raazia Tasadduq2, Jane B. Lian3, Janet L. Stein3, Gary S. Stein3 and Abdul Rauf Shakoori1*
...with their long-term use safety concerns, our research group focused on investigating efficacious, inexpensive and safe alternative therapies for osteoporosis. Investigating the osteogenic potential of CQ, our research group previously reported osteogenic stimulatory effects of CQ (hexane fraction). The present study explores anti-resorptive effects of CQ-H on bones. We utilized RAW264.7 murine macrophage cell line as osteoc...

Blessing Ndenum Peter1, Olufemi Bolarinwa Adedeji1, Reuben Chukwuka Okocha2,3*, Ekemini Moses Okon4 

... 80 ready-to-eat shrimps samples, Escherichia coli was isolated from 52 (65.5%), Salmonella spp. from 68(85.0%), Shigella spp. from 52(65.5%), Bacillus spp. from 52(65.5%), Staphylococcus aureus from 56 (70.0%) and Staphylococcus epiderdimis from 43 (53.8%) samples. All the Salmonella and Shigella isolates were sensitive to ceftazidime, cefuroxime, ofloxacin, and ciprofloxacin but resistant to gentamicin, cefixime, augmentin...

Yunilas1*, Muheri Indra Aja Nasution2, Edhy Mirwandhono1, Adi Fathul Qohar3 

... pigments, so it is necessary to do processing to reduce the chitin content first by fermentation using organic acids. Therefore, this study aimed to determine the effect of using organic acids (propionic acid and formic acid) and various fermentation times in reducing the BSFP chitin content. Completely Randomized Design (CRD), factorial 3 x 3 with three replications. Factor I was various doses of organic acid (3%, 6%, 9%), and Factor II was different ferment...

Razia Sultana1, Shinawar Waseem Ali1*, Ghulam Murtaza2 and Shahid Mahmood2

...ing (NGS) method. Yogurt samples were collected from open-air shops located in different areas of Pakistan. All yogurt samples were mixed to make one composite sample. DNA was extracted from yogurt using the phenol-chloroform (organic) method. Extracted DNA was used to perform NGS/Illumina high-throughput sequencing of hypervariable regions (V3 and V4) of the 16S rRNA gene. In the composit...

Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar

...gical instruments, and unsafe injections. Globally a total of about 170 million people have been afflicted with this malady per year and affects approximately (6%) of Pakistan’s population. HCV is RNA virus and there are seven primary genotypes and 67 subtypes in it. Determination of genotype is important for treatment and medication response. This study aimed to calculate the frequency of HCV infection and pattern of genotype distribution in suspected i...

Muhammad Haseeb Malik2, Muhammad Moaeen-ud-Din1*, Ghazal Kaukab Raja2 and Feroza Hamid Wattoo2

... 48 unrelated males were sampled for each Sahiwal and Crossbred cattle respectively. Candidate molecular markers present in Sahiwal but absent in Crossbred and vice versa were detected using amplified fragment length polymorphism method. Eleven markers were developed that were converted to single nucleotide polymorphism markers for high throughput genotyping. The allele frequencies in both breeds were determined for discrimi...

Khalid Mahmood1*, Muhammad Akbar Khan1, Sayyed Ghyour Abbas1,2, Muhammad Adeeb Babar3 and Muhammad Asim4

...ation) is assigned to Sansanosmilus rhomboidalis based on the morphology of canine. The recovered material is unique and rare in the Siwaliks of northern Pakistan. It also increases the stratigraphic range of this barbourofelines species from the Chinji Formation to the Nagri Formation.

...

H.A. El-Nagar1, A.M. El-Hais2, M.S. Mandouh2, W.M. Wafa1*, A.H. ABD El-Aziz, K.A. Attia4 

...ck seed oil (BS, Nigella sativa), or their combination on growth performance, immunity, and health status of females of newborn Friesian calves. A total of 20 newborn calves (37.51±0.92 kg LBW) were divided into 4 groups. During the suckling period, animals in the 1st group were fed a starter without any addition (G1, control). Animals in G2, G3, and G4 were fed the same starter supplemented with BP (10g/kg), BS oil (...

Kusuma Adhianto*, Mika Tania Sidabutar, Liman, Arif Qisthon 

...re as follows: P0: 80% basal feed + 20% corn cobs without ammonia (0% urea), P1: 80% basal feed + 20% ammoniated corn cobs (2.5% urea), and P2: 80% basal feed + 20% ammoniated corn cobs (5% urea). The variables observed included body weight gain, feed consumption, feed conversion, and income over feed cost (IOFC). The results indicated that there were no significant differences in feed con...

Di Zhou1, Qingmeng Long1*, Rong Yang1, Xiaoshan Tan1, Jun Li1, Mingyan Tang1, Zhonghai Zhao3, Ye Ao2, Zhinan Zhou1 and Changxue Chen1

...issues of goats with the same pedigree and analyzed the results using bioinformatics and qRT-PCR. We found 8120 differentially expressed genes (DEGs) in ovarian tissues (3205 up- and 4915 down-regulated), 5255 DEGs in the fallopian tubes (1317 up- and 3888 down-regulated) and 5180 DEGs in uterine tissue (2597 up- and 2583 down-regulated). The biological processes associated with these DEGs were primarily cell differentiation, reproduction and biological regula...

Salma Jameel Askar 

...received a single oral dosage of (0.1ml/100gm ) B.W. for six weeks. Red blood cells count, platelets counts, packed cell volume (PCV) percentages, and hemoglobin (Hb) concentrations, clotting times, levels of uric acid and liver enzymes were analyzed. RBCs, platelets, PCV%, and Hb concentration all decreased significantly at in the G2 compared to G1 while clotting time, serum uric acid, blood urea nitrogen levels, and liver enzyme functions all increased signi...

Wafaa AL. Bnyean1,2 ,Zainab A.H. AL-Mousawi1* 

...ontrol group(G1): Normal saline was given orally to rats. Positive control group(G2): rats for seven days were treated with Hydrocortisone Sodium I.P. (50 mg/kg). Therapeutic group(G3): rats for seven days were treated with Hydrocortisone Sodium I.P. (50 mg/kg) and then 14 days with GA (100 mg /kg) orally. Protective group(G4): Rats were treated for seven days with hydrocortisone Sodium I.P. (50 mg /kg) with GA for 14 days(100mg/kg) orally. glycyrrhizic acid G...
Muhammad Abdul Mannan1,2, Sharmin Chowdhury2, Md Abul Hashem3,4 and Md. Hazzaz Bin Kabir1,5*
...an area in Bangladesh. A sample of 400 abomasa (150 from sheep and 250 from goats) was collected from five different slaughterhouses in the Chattogram Metropolitan area and analyzed using morphological and microscopic methods. The Polymerase Chain Reaction (PCR) was then employed to amplify the sec internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA and the mitochondrial nicotinamide dehydrogenase subunit 4 gene...

Nabi Ullah, Abdul Samad Mumtaz*, Lubna Anjum Minhas, Muhammad Kaleem, Rooma Waqar, Amber Jabeen and Ayesha Hanif

...ented. Additionally, the sampling sites underwent thorough water physico-chemical analyses to explore any potential correlations with the diversity of phytoplankton. Conducted water physico-chemical analysis revealed that phytoplankton diversity greatly affects by various factors like Total soluble salts, Total Alkalinity, pH, TDS, EC, Temperature, Carbonate CO3-2, Bicarbonate HCO3-1, Chloride Cl-1, Sulphate SO4-2, NO3-1, Ca...

Mahmoud Mohamed Ahmed Youssef and Wafaa Mohamed Abd - El-Hameed El-Nagdi

...5.0 and 10.0 % were bioassayed against root-knot nematode, Meloidogyne incognita second stage juveniles (J2s). The obtained results revealed that the tested extracts at 10% caused 100% nematode mortality at 72 h of exposure followed by the other concentrations with the percentages of net mortality ranging from 49 at the lowest concentration of mashed leaves to 100% at the highest concentration of mashed leaves and flowers. Under screen house conditions, the

Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*

...ry cattle and poses colossal economic losses to the farming community worldwide. The role of vaccination in controlling mastitis is of paramount importance and significantly reduces the use of antimicrobial agents. This study aimed at preparing and evaluating inactivated polyvalent mastitis vaccines containing bovine origin Staphylococcus aureus (tst), Streptococcus uberis (cpn-60 targeted STUB), and Escherichia coli (aggR) in a rabbit model. Four types of mas...

Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li

...linical detection of 150 samples, collected form calves in Hebei Province, by multiplex PCR were the same as those obtained by colloidal gold test paper detection. We discovered that the co-infection rate of BRV and BCV was 41.3% (62/150), of BVDV and BRV 8.0% (12/150), of BVDV and BCV 6.0% (9/150), and of BVDV, BRV, and BCV 10.0% (15/150). In our clinical samples, mixed infection of BRV a...

Swagata Das Gupta1, Majharul Islam1, Towhida Kamal2,Md. Rayhan Faruque3,Md. Shohel Al Faruk4* 

... food and water from the same source, clinical investigations showed greenish diarrhea, and post-mortem studies revealed hepatomegaly, congested heart, and proventriculus, and found nodules in the crop wall as tentative diagnoses. Tropozoid was identified through microscopic examination by direct wet smear and giemsa staining. The cankers were treated orally with metronidazole at a dose of 20 mg/kg body w...

Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1 and Abdur Rahman Ansari1

...tion through molecular assays. 

...

Asmaa A Darwish* 

...seased group (DG). Blood samples were collected from both groups and immunological and clinicopathological parameters were estimated and statistically analyzed. Diseased rams displayed a significant (P<0.05) increase in the pro-inflammatory cytokines, acute phase proteins, free radicals, globulin, triglycerides, kidney function tests, and liver enzymes concentrations and a significant (P<0.05) decrease in IL-10, antioxidants, total protein, albumin, gluc...
Durairaja Ramulu1*, Jawahar Paulraj2, Jayakumar Natarajan1, Sudhan Chandran1 Sudhir Kumar Das3, Suresh Eswaran4, Santhakumar Rajagopal5 and Padmavathy Pandurangan6
...barb, Dawkinsia filamentosa (Valenciennes, 1844) (L∞ 22.05 cm, K 0.68y-1) and Roho labeo, Labeo rohita (Hamilton, 1822) (L∞ 47.25 cm, K 0.71y-1) and the mortality parameters of C.mrigala (Z 2.14 y-1, M 1.22 y-1 and F 0.92 y-1), D. filamentosa (Z 2.42 y-1, M 1.54 y-1 and F 0.88 y-1) and L. rohita (Z 2.87 y-1, M 1.42 y-1 and F 1.46 y-1). The estimated exploitation ratios (E) for the three species were 0.43, 0.36 an...

Naeem Akhtar1*, Sana Noureen1, Muhammad Asif2, Ahsan Aziz2, Usman Saleem1, Talat Mahmood3, Nadeem Raza4 and Waqas Raza5

...tic factors, sugarcane mosaic virus (SCMV) is one of the most devastating factors that causes significant losses of sugarcane production. Here we are reporting the assessment of genetic variation among sugarcane accessions based on morphological parameters, SCMV severity, incidence and resistance/tolerance. The experiment was conducted in College of Agriculture, University of Sargodha, Sargodha, Pakistan using randomized complete design in triplicate. Differen...

Ahmed Jaafar Mousa*, Nothaila Rasheed Hamid 

...5.8s rRNA-ITS2 gene. 100 samples were collected from racing pigeons between September and December 2022. The swabs were taken from the throat, pharynx, crop, and esophagus with a cotton swab. Utilizing direct smear and polymerase chain reaction (PCR) techniques, 30 (30%) of the 100 collected samples were found to be positive. A DNA sequence of 372 bp length that partly encompassed the coding region of the ITS1-5.8s rRNA-ITS2...

Mustafa M. Khalaf*, Rana A. Salih 

...stress, its therapeutic usage is restricted. The most potent antioxidant among flavonoid chemicals is quercetin, the aim of this study was to evaluate the protective effect of quercetin against cyclophosphamide. An explanation of quercetin’s hepatoprotective effects against cyclophosphamide-induced hepatotoxicity. Twenty-eight male Westar rats (that weighed 200–250 g) were chosen at random and divided into four equal groups for this investigation. ...
Mohamed I. El-Katcha1, Mosaad A. Soltan1, Seham M. El-Kassas2*, Mahmoud M. Arafa3, El-Sayed R. Kawarei 3, Karima M. El-Naggar1*
...undred, 55 weeks old of Isa Brown hens were randomly allotted into 5 groups. First group received CuO at 8 mg/kg diet, while the second and third group supplemented with the organic form of Cu (copper polysaccharide complex) at 8 and 4 mg/kg diet, respectively. Fourth and fifth group received the Cu as nanoparticles (CuO-NPs) at the same levels of the organic form, respectively. Birds fed ...

Wen Xiong1*, Shengao Chen2, Dong Xie3, Qiang Wang4, Yanxia Li5, Lei Pan6, Xiaolei Ren7*, Wei Tang8*, Kang Chen9 and Ross N. Cuthbert10

...habitat loss, pollution, sand mining, over-fishing and non-native species as the major threats to freshwater fish biodiversity in this system. Thus, we propose that protected areas and bans on fish introductions, should be adopted and better enforced in this and other freshwater systems.

...

Weidong Liu, Jiashen Tian, Zhen Wang, Zhongren Kong, Jiabo Han and Zhichuang Lu*

...s were compared with the same population in 2005. The haplotype diversity of Liaodong Gulf spotted seals was low compared to other spotted seal populations in other habitats. Fortunately, the genetic diversity of the current Liaodong Gulf spotted seal population was higher than 15 years ago, and the population expanded between 2005 and 2020. Our findings indicated that conservation activities for Liaodong Gulf spotted seals in recent decades have been valuable...

Syed Shakeel Shah1, Ayesha Jameel1, Sabila Afzal1*, Muhammad Zubair2, Iram Fatima Bokhari1

...llected from shops, wholesale markets and vendors in Narowal were analyzed for parasitic contamination. Five vegetables, including coriander (Corriandum sativum), spinach (Spinacia oleracea), mint (Mentha viridus), green chili (Capsicum annum) and carrot (Davcus carota), were examined in our study. One hundred forty-five samples of different vegetables were collected and processed. 200 gra...
Jaffar Hussain*, Zeenat M. Ali and Syed Farman Ali Shah
...aline catalyst undergoes saponification reaction when free fatty acid concentration was high. The acidic catalyst preparation was expensive and complicate exhibited slow rate of reaction. The biodiesel used in blend form and diesel engines required the modification. The biodiesel sustainable and release no greenhouse gas. 
...

Ihab G. AL-Shemmari1*, Ali Hussein Fadhil1, Mohammed Assad S. Alkabi1, Eman Jawad Jabber2 

...blood and uterine tissue samples collected from 139 brucellosis-infected ewes. Results. The PCR identified 37 (26.61%) positive cases of brucellosis, which was less than the 41 (29.49%) instances identified by the Rose Bengal test and greater than the percentage identified by the culture, which was 35 (25.1%). In comparison to the results of the culture test, the polymerase chain reaction test was found to be (89.19%) sensitive, (98.04%) specific, and (95.68%)...
ZAMARA MASOOD1, FAROOQ SALEEM1, SARFRAZ AHMAD2, TALHA JAMSHAID3, IMRAN WAHEED4,
MUHAMMAD AZEEM1, UMAR FAROOQ GOHAR5 & HAFIZ MUHAMMAD ARSALAN6, 7*
...des, cardiac glycosides, saponins, flavonoids, cumarins, steroids,
proteins, and amino acids, fixed fats and oils in all fractions. Floral
extracts were found to contain highest total phenolic contents (64 to 73
mg GAE/g), whereas leaves n-hexane fraction exhibited maximum DPPH
potential (70 %). All the extracts showed promising antimicrobial activity
in terms of zone of inhibition against selected ba...
KHALID JAVED IQBAL1, ARSHAD JAVID2, NOOR KHAN3, IRFAN BABOO4, AHMED ALI5, KASHAF
MOHSIN6, AZRA ANWER7, USMAN ATIQUE8, MUHAMMAD ALTAF9, DILAWAR HUSSAIN10 & ASMA
CHAUDHRY11
...nsumer interests in food safety and to prefer fish food
seasonally have also shown significant percentage effects on consumer‘s
attitudes towards fish consumption.
...

MAH NOOR KHAN, NABILA ROOHI, BILAL JAVED & MUHAMMAD AMIR IQBAL*

... healthy controls of the same age range were recruited from University of the Punjab, Lahore. A significant decline (P ≤ 0.05) of T3 was seen in automobile workers as compared with controls. Both, automobiles workshop workers and petrol station workers demonstrated a significant decline of T4 as compared to controls. Mild elevation of TSH was present in both Automobiles and Petrol filling Station workers as compared to controls. Reduced levels of T3 and T4 ...
MISBAH RAZZAQ, MUHAMMAD RAIS*, MUHAMMAD ARSLAN ASADI, SALEHA ABBASI &
ABDULLAH IBRAHIMv
...t plant species in fecal samples and
stomach contents, respectively. The majority of insects consumed by the
lizard species were Coleopterans (0.22 ml) followed by Hymenopterans
(0.18 ml) and Hemipterans (0.18%). The similarity index showed that the
similarity in food items at sites “rocky habitat-human habitations” and
“rocky-stream habitat” was highest (38% each) followed by “human
habit...

SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN

...gh in-vitro biological assays.
Quantitative analysis was done on powder of Misopates orontium by
using standard methods for estimating the primary and secondary
metabolites. The maximum value of total carbohydrates (11.04μg/ml),
total starch (14.69μg/ml), total protein (14.34μg/ml), total amino
acids(28.43μg/ml), total lipids (3.67 μg/ml), total glycosa
FARZANA SIDDIQUE*1, NAZIMA FIRDOSE2, MUHAMMAD ARSHAD2, WARDAH HASSAN2
& SHAFAAT YAR KHAN2
...raw cow
milk samples from citrus plantation zone in district Sargodha were
collected and analyzed for the presence of deltamethrin and malathion
pesticides using HPLC technique. Results showed that 100 % of milk
samples were contaminated with pesticide residues. Maximum
concentration of malathion (0.72±0.02 μg/kg) and deltamethrin (0.75±0.04
...

ZULNAIRAH REHMAN1*, AZMATULLAH KHAN2, ABDUL WAJID3 & AYESHA MAQBOOL4

...lness called
salmonellosis. It is characterized by the typhoid fever and gastroenteritis.
To investigate the prevalence of Salmonella 240 samples were collected
from different areas of District Mianwali. Out of 240, eighty samples were
of commercial eggs, eighty were of non-commercial eggs and eighty were
of egg storing tray

SAGHIR AHMAD*, WARDA HANIF & SANA YOUNAS

...to April 2017. 640 blood samples were taken from sheep, goats, farmers and non-farmer humans (160 each). Blood sera were examined to detect anti-Toxoplasma antibodies (IgG) by employing latex agglutination test (LAT). The findings showed the seroprevalence of T. gondii 36.25% (58/160) for sheep, 28.1% (45/160) for goats, 21.2% (34/160) for farmers and 6.8% (11/160) for non-farmers. A significant difference (P-value 0.00; OR: 3.655; CL: 1.77-7.51) was found bet...

MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR

...ofloxacin in injection dosage form. The proposed method is free
from expensive solvents, chemicals and time consuming steps and
can be used in quality assurance laboratories of pharmaceutical
industries.
...
ZAHID FAROOQ1, RUQIA BIBI2, IRFAN BABOO3*, KAMRAN ABBAS4, MUHAMMAD SHAHBAZ5, KHALID
JAVED IQBAL6, DILAWAR HUSSAIN7, MUHAMMAD ABRAR8, ASMA CHAUDHRY9 & TANVEER
HUSSAIN10
...div>
egested fecal samples (n=100) from six different captive facilities were
collected. The prevalence of endoprasites among all the Indian peafowl
ranged from 46.6%-66.7%. The highest prevalence was recorded in black
shoulder peafowl 66.7% followed by pied peafowl (66.1%), white peafowl
(59.1%) and common peafowl (46.6%). The prevalence of endoparasites
was highest 74.3% at Gatwala Wildlife Pa...

ZAHOOR AHMAD SAJID1* & SHEZA AYAZ KHILJI2

...eplicate plants for each salt (NaCl) treatment. Initially the plants were raised by irrigating with tap water and then after their establishment, with NaCl (50, 100 and 200 mM) containing water. Various growth and biochemical characteristics were found to be adversely affected by saline treatments i.e., root length, shoot length, fresh weight, dry weight, amount of protein and antioxidant enzymes. The higher levels of NaCl (...

SHEIKH AJAZ RASOOL1*, MUNAZZA DANISH AJAZ2, MUHAMMAD SALMAN RASOOL3, AHSAN SATTAR SHEIKH4 & FIRDAUS KOUSAR BUKHARI5

...against Campylobacter nuisance in humans and poultry. In addition, a few probiotics have been instrumental to cut down the adverse effects rendered due to the classical antibiotic therapies with particular reference (wpr) to GIT. Particular probiotic strains (including Lactobacillus johnsonni La1 and Saccharomyces boulardii) have been able to downgrade the concentration of bacteria. It has been reported that Lactobacillus reuteri is at par in efficacy in this ...

KHADIJA SUMMIA*1, ROHEELA YASMEEN*2, ALEEM UN NISA3, SAMIA DJEFFAL4 & TAHIRA JABEEN1

... around Lahore. Total 50 samples were collected from two different localities of Lahore and categorized in two groups: Group A (Raiwind, 30 samples), Group B (Bedian Road, 20 samples). All samples were analyzed for estimation of aflatoxins level using thin layer chromatography (TLC) method. The results showed that out of 50 sa

Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani 

...y methods with purposive sampling techniques for determining the research location. We observed variables such as body weight, chest circumference, body length, and shoulder height. Data analysis was carried out descriptively based on arithmetic mean, standard deviation, and coefficient of variance. The result showed that the body weights of male and female buffalo crosses at <6, 7−12, 13−18, and 19−24 months were 210.26 and 196.48, 304.14...

Muhammad Syarif Djaya1, Osfar Sjofjan2, Hartutik Hartutik2, Irfan Hadji Djunaidi2 

...sed in this study. The basal diet was used in this study T0: basal diet, T1: basal diet + 10% lutein, T2: basal diet + 20% lutein, T3 basal diet + 30% lutein, T4 : basal diet + 40% lutein. A completely randomized design was adjusted with five treatments and six replications for each...

Zainab Sattar Ali* , Safa Azhar Razzaq , Layla Hammody Hashem 

...0 mg/kg of propolis. The samples of blood had gathered from rats each three weeks for assessed the concentration value of FSH, LH, testosterone, and inhibin B hormone for each of groups above. At the termination of experiment were taken in from them specimen of testes for a purpose pathohistology study and assessed the percentage of DNA fragmentation. The current study observed damage in this sperms DNA and alteration of histopathological section. In addition ...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

...ce of alkaloids, phenol, saponins, flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) and MBC/MIC ratios (2.0-4.0). Furthermore, a time killing curve of each 4x MICs and 8x MICs concentrations of juice extract showed a distinguished bactericidal effect at 6th hr. Therefore, the use of citrus fruit juice as medicine to treat infections ...

Saloua Ziani1*, Khalid Sohaib2 , Youssef Lhor3, Ikhlass El Berbri4, And Ouafaa Fassi Fihri4  

...oonosis caused by the Lyssavirus, and dogs are the primary host and reservoir. For the international movement of pets, rabies-free countries require a rabies quantification before importing animals, which varies significantly. The objective of this study is to investigate the reasons for variations in antibody titers in dogs. Data from 848 dogs exported from Morocco was collected. The results revealed that too-old dogs (over six years) or too young (under four...

Nusrat Habiullah1*, Shah Nawaz Kumbar1, Fahmida Parveen Samo1, Shamusuddin Bughio2, Asghar Ali Kamboh3, Burirah Rehman Talpur1 

...on, the liver and kidney samples were collected for gross and histopathological examination. The mild clinical signs like reduced feed and water intake, and weight loss in group C, D, E and F was noted; whereas diarrhea, muscular tremors, and lethargy were observed in group C and D. Significantly increased (p <0.05) GGT, bilirubin, creatinine and uric acid levels were recorded in C and D (lead toxicity groups), as compared to control group; whereas group E...

Abdul Latif Rind1*, Shahid Hussain Abro1, Rameez Raja Kaleri2,3, Ghulam Mustafa Solangi4, Raza Ali Mangi5, Muhammad Anees Memon6, Depeesh Kumar Bhuptani7, Sana Noor8, Zainab Lanjar9, Zahid Ali Mangrio10 and Abdul Wahid Solangi

...h, Pakistan. Two hundred samples of oculo-nasal discharge were gathered and subjected to Ic-ELISA testing to identify PPR antigens. The analysis of PPR prevalence was carried out according to breed, sex, age, and Taluka. The overall PPR prevalence was found to be 20%, with the highest prevalence rate (50%) observed in Jam Nawaz Ali Taluka and the lowest ...

Hazrat Usman1, Shakeel Ahmad2*, Salah Uddin3, Humaira Wasila3 and Yasser Durrani4

... Pakistan. A total of 21 samples of well water were collected from the study area, with seven samples obtained from each Tehsil. The research analysis focused on various parameters, including pH, turbidity, electric conductivity, total hardness, sodium, potassium, calcium, magnesium, carbonates, bicarbonates, chlorides, and fluorides. The recorded average value ranges for each parameter provided insights into the characteris...

Qasim Ali1, Mudssar Ali1*, Muhammad Awais Ahmad1, Asif Sajjad2 and Shafqat Saeed

...was recorded for Apis dorsata, followed by E. balteatus and Ischiodon scutellaris. Comparative analysis revealed that fruit dimensions, weight, pulp content, and seed count per fruit were notably improved in instances where insect pollination was facilitated, in contrast to cases where no insect visits were allowed. Furthermore, chemical properties were also found to be superior in scenarios involving free insect visits. The study underscored the efficacy of A...

Abdiaziz Idiris Mohamud1,2*, Yonis Abukar Mohamed1,3 and Shafii Abdullahi Mohamed1,4

...ease prevention, is necessary for evidence-based decision-making. By leveraging research, promoting evidence-based interventions, and strengthening policy and governance frameworks, Somalia can enhance animal welfare, support sustainable livelihoods, and contribute to overall sustainable development in the country. The integration of animal welfare into Somalia’s sustainable development agenda will not only benefit animal well-being but also foster resil...

Muhammad Umar Farooq1, Kashif Ishaq1*, Muhammad Imran Khan1, Muhammad Farooq Iqbal1, Tanveer Ahmad1, Jamil Akbar1, Asim Fraz2, Sara Naeem1 and Aansa Latif1

...erformance using a compensatory growth tool. A total of twelve male Beetal kids with an average weight of 20±2 kg and approximately 6 months old were randomly selected and kept for 85 days including 15 days of an adjustment period. There were two phases of the experiment. During the restriction phase; the animals were divided into three groups i.e. T1=feeding @2% of body weight (BW), T2=feeding @3% of BW, and T3=feeding @4% of BW. In the re-alimentation...

Shakirat Bolatito Ibrahim1, Raheem Olatunji Aminu1,2*, Aisha Olushola Arowolo1 and Adams Sanusi Musa1

... average distance of the sampled households to public and private hospitals in the study area is 2.33km and 0.88km. Most households consume cereals, roots, and tubers. As indicated by the household dietary diversity score, 85.64% of households showed a high level of dietary diversity. The food expenditure data shows that food insecurity affected 59% of households, with levels of severity and depth of food insecurity of 0.27 and 0.19, respectively. The ordered ...

Ali Mosa Rashid Al-Yasari1, Zahid I. Mohammed2, Haider S. Almnehlawi3,4, Ali F Bargooth5, Nawar Jasim Alsalih1, Huda F. Hasan6*, Mohenned A Alsaadawi1 

...d glucagon. Animals were sacrificed on day 28, and tissues were collected for immunohistochemical identification of pancreatic cells that produce insulin and glucagon. Based on our results, none of the research parameters significantly differed across the groups on day 0. Days 1 through 28 showed that STZ-Diabetes group had the uppermost blood glucose level with lowermost blood insulin among the study groups as a result of the induction of diabetes. Comparing ...

Hui Fang*, Kai Xue and Teng Yang

...sease (ADPKD). Fifty RNA samples from Gene Expression Omnibus (GEO) database were obtained to identify differentially expressed miRNAs (DEMs) and genes (DEGs) in the progression of ADPKD. Functional annotation and enrichment analysis of DEG were performed. iRegulon was used to construct the miRNA-mRNA network and predict related transcription factors (TFs) associated with ADPKD. The function of miRNA network was explored by analyzing GO and KEGG enrichment. Fu...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...rom leaf, peel, and stem samples. The percentage yield of BRM was determined in the range of 60 to 93 % from these waste parts of the plant. This study achieved 4, 6, and 7-fold purification for leaf, peel, and stem BRM, respectively. This study showed positive results for utilizing affinity-purified BRM enzyme in the laundry and detergent industry due to its stability in different detergents and destaining properties.

...

Jyotsana Shakkarpude1*, Aditya Mishra2, Deepika D. Caesar2, R.K. Sharma3, Anand Kumar Jain2, Sanju Mandal2, Rajesh Kumar Vandre4, Danveer S. Yadav5, Bhavna Ahirwar6 and C.P. Solanki6

...postpartum. 5ml of blood sample was collected from animal for analysis of superoxide dismutase, total antioxidant capacity, HSP70, albumin/globulin ratio and immunoglobin G. The overall mean values of plasma superoxide dismutase, total antioxidant capacity and immunoglobin G of high betaine group was numerically higher than other groups which differed (p<0.05) significantly between groups. The overall mean value of plasma HSP70 of high betaine group was min...
Ummul Firmani1,3, Rahmi Nurdiani2, Arning Wilujeng Ekawati2 and Happy Nursyam2*
...mined using amylolytic assay, synergistic activity, hemolytic activity, and antagonistic activity. The results showed that there were several types of bacteria found in the digestive tract of milkfish. One of these bacteria could produce an amylase enzyme with an amylolytic index of 5.16, identified as Bacillus paramycoides. This bacteria also synergized with other bacteria and did not have hemolytic activity on blood agar media. The results of the antagonisti...
Saad Ibrahim Al-Sultan1, Mariam H.E. Khedr2, Ahmed S. Abdelaziz3, Mostafa M. Abdelhafeez4, Tamer Mohamed Gad5, Sabry Mohamed El-Bahr6,7*, Sherief Abdel-Raheem1,8 and Hesham A. Khalifa3
...als, vitamins, and polyunsaturated fatty acids. Toxic metals such as lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg) are of no-known physiological importance. The objectives of the present study were to quantitatively estimate the residual levels of Pb, Cd, As, and Hg in camel meat and edible offal including round, liver, kidney, and tongue in samples collected from Zagazig slaughterhouse, Egypt and Al-Ah

Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*

Urosevic Milivoje1, Matejevic Milosava2*, Mandic Radomir3 and Dameski Pance4

...Study was conducted on a sample consisting of 38 mouflon horns from hunting grounds in Serbia. The parameters that were analyzed (length of the left horn, length of the right horn, circumference of the horns, spread of the horns, color of the horns, ring and curvature) were measured according to the propositions adopted by the The International Council for Game and Wildlife. The aim of the paper was to determine the absolute and mean values ​​of the length...

Sha Li1,2, Yacheng Hu1,2, Wenjing Li3, Wei Su1,2 and Wei Jiang1,2*

...angtze River, China. The samples were collected with gill nets (2×50 m, mesh size: 40 and 60 mm) between November 5th, 2019, and December 31st, 2019. A total of 1252 specimens were used to estimate the related parameters. The LWRs parameters of all nine species were found to be significant (P <0.05). The values of b in LWRs ranged from 2.455 to 3.009. New maximum total length (TL) and standard length (SL) records for two of the nine species were repor...

Wafa Akram1, Shahan Azeem2, Shafqat Shabir1, Haroon Akbar1*, Warda Gill3 and Muhammad Imran Rashid1*

...i that is transmitted by sandflies (Phlebotamine flies). In November 2020, a case of leishmaniasis was diagnosed in a captive tiger through microscopy and L. infantum was confirmed by PCR and sequencing analysis. DNA sequencing of the amplicon revealed close homology with Leishmania sequences available in GenBank. Alignments and phylogenetic analyses of the Leishmania infantum from a tiger in Pakistan indicated 94-100 % identity with Leishmania from animals an...

Al Salihi karima Akool1* , Almas. M. Al-Bayati2, Iman Mousa Khaleel3  

...). Sixteen mammary gland samples and its teats ( 8 SCPMG and 8 SCN-PMG) were collected from Al Muthanna abattoir. Gross examination, and full description were done and recorded. The samples were dissected and kept in a 10 % neutral buffered formalin. All samples were processed by histological technique and stained with Haematoxylin and Eosin. This study showed that SC’s udder appeare...

Vijay Kumar1, Asghar Ali Kamboh1*, Nazar Ali Korejo1, Ambreen Leghari2, Faiz Muhammad Khand2, Riaz Ahmed Leghari1, Khaliq-ur-Rehman Bhutto3 

...ks. A total of 125 feces samples (55 broilers, 45 layers and 25 ducks) were collected in district Hyderabad. Isolation was done according to ISO 6579 standard method. Identification of isolated bacteria was done using the Salmonella specific biochemical tests. Antimicrobial resistance patterns were determined using the disk diffusion method. Salmonella were detected in 22, 19 and 10 samples, showing prevalence of 40.0, 42.22...

Nur-E-Farjana Ilah1, Md. Joynal Abedin2, Esmout Jahan Alice1, Taiba Akter Laboni1, Mst. Shahinur Khatun1, Most. Shakila Sarmin1, Md. Ashekur Rahman1, Obaidur Rahman1, Md. Akhtarul Islam1, Muhammad Ali Fardoush Siddquy3 and Md. Yeamin Hossain1*

Waseef Ullah Khan*, Yasser Durrani and Muhammad Ayub

... Results showed that all samples retained total soluble solids between (17.89-20.80˚Brix), pH (4.06- 4.91), color (6.94 to 8.01), flavor (5.34 to 6.34) and taste (5.59-6.99). The results concluded that OLE can serve as a dual-purpose solution, acting as a natural antioxidant and antimicrobial preservative for refrigerated mango OLE blend.

...

Muhammad Shahid Nisar1*, Ahmad Kamran Khan1, Ghulam Fatima2, Irfan Ahmed4, Khizar Sami Ullah2 and Syeda Amber Hameed2,5

...treatment 1) with 100% basal feed (BF) and 0% fruit waste (FW), diet 2 (D2) (75 BF + 25% FW), diet 3 (D3) (50% BF + 50% FW) and diet 4 (D4) (25% BF + 75% FW). Non-significant results (p>0.05) were found in feed intake and body weight gain and carcass characteristics in all treatment groups while feed conversion ratio (FCR) were resulted significantly difference (p<0.05) in D2 (1st, 3rd, 4th, 5th week), D3 (1st and 3rd week) and D4 (1st, 2nd, 4th and 5th ...

Aamir Ali, Hafiz Muhammad Tahir*, Azizullah, Shaukat Ali, Muhammad Farooq Bhatti, Muhammad Summer and Ali Haidar Gormani

...i-diabetic and antidepressant activities. Further studies should explore the mulberry leaves potentials as these plants have got the attention of both the pharmacological and commercial industries.
 
Novelty Statement | The mulberry leaves possess various therapeutic potentials. In this study, we have summarized all the therapeutic potentials of different mulberry leaves extracts. Moreover, the importan...
Nighat Nisar*, Hasina Maham Qadri, Asad Afridi, Muhammad Sami Khan, Muhammad Abdullah Khan
...students’ level of satisfaction towards services provided by cafeterias in public sector medical institute of Karachi, Pakistan. A cross sectional study design was adopted with a sample size of 318 round of to 400 and by using qouta sampling. A self-administered questionnaire was used to collect data regarding cafeteria use, food consumption, frequency, waiting time, cost, ambience, ...

Maria Endo Mahata*, Yose Rizal, Zurmiati, Sepri Reski

.... We aimed to reduce the salt concentration of the brown seaweed P. australis by immersing it in flowing water. We used a completely randomized design (CRD) with different immersion durations (0, 4, 8, 12, 16, 20, and 24 h) of P. australis in flowing water. Each treatment received four replications. We evaluated dry matter (DM), organic matter (OM), crude protein (CP), ash, salt, and alginate contents. The results revealed a...

Rokhani Rokhani1, Amam Amam2*, Mochammad Wildan Jadmiko2, Dhimas Yusantoro2

...velopment in the One Thousand Cattle Village Program. The research variables consisted of farmer empowerment (X) and five dimensions of sustainable livestock development, including the ecological dimension (Y1), economic dimension (Y2), social and cultural dimension (Y3), institutional dimension (Y4), and technological dimension (Y5). Data were collected using surveys (interviews and questionnaires), observation, and Focus Group Discussion (FGD). The responden...

Mervat M. Fath- Allah, Amal A. Ahmed, Hala A. Amin

...>...

Nguyen Thi Thu Hien1, Dong Huu Rin2, Nguyen Xuan Hoa1, Le Viet Tuan Khanh2, Phung Thang Long1*, Dinh Thi Bich Lan1

...DNA of fresh lung tissue samples from PEP infected pigs was cloned into pGEM®-T Easy vector for sequencing, and then was inserted into pET28a vector to express the protein. The cloning result revealed that the gene encoding the M. hyopneumoniae P36 protein was 432 bp in length, and 99.07% similarity to the nucleotide sequence of P36 gene from strain M. hyopneumoniae 7448 recorded in GenBank (accession No. AE017244.1), corresponding to 144 amino acids. Expr...

Rini Widyastuti1*, Rangga Setiawan1, Nurcholidah Solihati1, Siti Darodjah1, Kundrat Hidajat1, Mochamad Ali Mauludin2, Alkaustariyah Lubis3, Mas Rizky Anggun Adipurna Syamsunarno4, Sigit Prastowo5, Takdir Saili6, Arief Boediono7

...est Java, Indonesia. The sample population consisted of thirty participants and the reproductive performance of 96 goats was investigated. Sociodemographic characteristics and reproductive knowledge of breeding were collected through questionnaires, interviews, and direct observation of the farms. The majority of members of Simpay Tampomas Farmers Group were males aged 41-59 years, who graduated from elementary school and had more than 10 years of experience i...

Sri Suharyati, Tiwi Aries Pani, Akhmad Dakhlan, Syahrio Tantalo, Kusuma Adhianto*

Nur Saptahidhayat1, Claude Mona Airin1, Yanuartono1, Dyah Ayu Widiasih1, Soedarmanto Indarjulianto1*, Sri Handayani Irianingsih2, Meta Iqomah3

...e fed a combination of basal feed and Karfeed concentrate, Group II, cows had a variety of basal feed, Karfeed concentrate, and premix, and Group III, cows got a combination of basal feed and Protelis® concentrate. The study had 55 days of treatment. The evaluation of milk production and quality was conducted before and after treatment. The research showed that all cows were diagnosed ...

Umar Salisu Ahmad1, Adamu Abdul Abubakar1,2*, Hassan Abubakar Bodinga1, Nura Abubakar1, Ekaete Ime Oviawe1, Zaid Shehu3, Abubakar Musa Mayaki3

... ocular discharge, foamy salivation, and labored breathing were observed. The prescapular and submandibular lymph nodes were enlarged, and a hard mass was palpable on the distended abdomen. Rectal examination revealed hard rectal feces in rectum were found. The rectal temperature was subnormal (35.4 oC), respiratory rates were 12 cycle/min and the pulse rate was so weak that it was not detectable. Attempts to induce defecation using a KC-bloat failed, and the ...

Putu Henrywaesa Sudipa1*, Hapsari Mahatmi1, Ketut Tono Pasek Gelgel1, I Gusti Ketut Suarjana1, I Nengah Kerta Besung1, Romy Muhammad Dary Mufa2 , I Wayan Sukernayasa

...rphological methods. The samples were vaginal swabs obtained from 22 healthy female Bali cattle from various locations in Bali, Indonesia. For isolation, vaginal swabs were cultured on Sabouraud’s dextrose agar (SDA) medium and identification was performed macroscopically by observing colony growth and microscopically using the tape smear method stained with Methylene Blue stain. The results showed that 32 fungi belonging to seven genera were isolated fr...
...plants featured severe mosaic on Lentil cv. Giza51, mottling on cv.Giza9, mild mottle on cv. Giza 4, yellow mild mottling on cv.  Giza370. Seed transmission tests of PMoV confirmed of the virus transmission through Lentil seeds. Peanut Mottle Virus was detected in the testas, cotyledons and embryo dissected from seeds of each Lentil cultivates. Electron microscopy of PMoV showed virus particles aggregates and degenerated...

Iftikhar Ahmad1, Tahir Saeed1, Umair Faheem2*, Qaisar Abbas2, Muhammad Saleem Akhtar Khan3, Mussurrat Hussain2, Tanveer Ahmad4, Gulzar Akhtar4, Asifa Hameed5, Muhammad Hasnain6 and Muhammad Jamil7

... carried out on Jasminum sambac against flower thrips for efficacy of insecticides. To conduct this experiment, six insecticides viz., imidacloprid 20Sl, Spinosad 240SC, Spintoram 120SC, Chlophenapyre, Imidacloprid+fipronil and abamectin were applied. Thrips population was observed on jasmine flower before application of insecticides and thrips mortality data was noted after 24, 72 and 168 hours of insecticides spray. The ma...

Shafqat Ullah1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Raheela Taj3, Fatima Syed3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Muneeb Islam7 and Sumaira8

... group fed with normal basal diet group B was termed as negative control group offered basal diet + dexamethasone and group C, D and E was offered 0.2mg/kg, 0.3mg/kg, and 0.4mg/kg of basal diet, respectively. The trial lasted for 35-days and on the last day two chickens from each replicate were slaughtered (a cumulatively eight chickens from each group). Samples from different regions (duo...

Siddique Ahmad1*, Basit Ullah1, Sajid Ali2, Ali Zaid1, Zeeshan Ahmad3, Muhammad Usaid1, Muhammad Zeeshan1 and Saif Ullah3

... bio-aab, T10: Control), same treatments followed for without bio-aab. OS have applied accordingly to the plots one month before sowing, bio-aab solution (60 litres ha-1) was applied with first irrigation to one experiment before sowing to encourage microbial action necessary for manure decomposition, while half N was applied at sowing time and half after seven days of sowing with first irrigation. Statistical analysis was d...

Salma Sharif1, Rana Arsalan Javaid2*, Abid Majeed2, Muhammad Shahzad Ahmed2, Qurat ul Ain Sani2, Faiza Siddique2, Muhammad Arshad3 and Niaz Ali

...al vegetation index, thousand grain weight and grain yield. The overall mean for Grain yield ranged from 1.71 tons/ha to 6.18 tons/ha. Grand mean of all genotypes for Grain yield was 4.1 tons/ha. Maximum Grain yield was observed in GSR 11 followed by GSR 10 and GSR 44 that were followed by 5.88 and 5.74 tons/ha respectively. Significant variation was also found in case of qualitative characters as obvious groups were formed based on visual observations. In PCA...

Sona Salem El-Nwehy1*, Assem Abbas Mohammed El-Naggar2 and Adel Badr El-Nasharty1

... P, and K production and saving one-fourth of the amount of chemical fertilizer, lowering production costs, and obtaining a high-quality product with decreasing pollution. 

...

Muhammad Raza Salik1, Muhammad Babar Shahzad Afzal1,2*, Ayesha Komal3, Muhammad Nawaz Khan1, Muhammad Ihsan Ullah4, Faheem Altaf5, Akbar Hayat1 and Hira Tariq1

Michelle Anne Diano1,2, Loel Dalan1,2, Neil Pep Dave Sumaya3 and Nanette Hope Sumaya1,2*

...n of Caenorhabditis briggsae (Nematoda: Rhabditidae) was recovered from the giant African land snail, Lissachatina fulica (Gastropoda: Achatinidae), in Bukidnon province (8.0515° N, 124.9230° E) of the southern Philippines. This species was identified by morphological characterization and analysis of the D2-D3 expansion segments of the 28S rDNA sequence. The D2-D3 rDNA sequence alignment of the four isolates, B2R1 (M...

Anshara Javed Qureshi and Ishrat Aziz*

...gated in May 2023. Fecal samples of 100 pigeons (30 males and 70 females) belonging to 30 different breeds were collected from four different houses of the Dilawar colony area of Bahawalpur, and proceeded qualitatively through direct microscopy and floatation method. In this study, 18 (11 males and 7 females) out of the 100 samples (with a prevalence 18%) were found infected with Capillaria spp. of nematodes. The qualitative...

Aamir Shakeel1*, Anwar Ali2, Tahir Iqbal1 and Ziad Raza1

...nd conversion. Grassland saw a modest increase of 1.94%, potentially resulting from natural processes and land management changes. An expansion of 5.59% was observed in the “Other Land” category, which may involve complex land use changes. The most striking change was a 158.82% increase in settlements, reflecting rapid urbanization and infrastructure development. Wetlands expanded by 4.62%, possibly due to both natural fluctuations and human interv...

Anwar Ali1*, Muhammad Ayaz Khan2 and Nowsherwan Zarif1

...a was collected from 253 sample plots using a systematic random sampling technique. The total carbon stock in the study area was estimated as 1,374,702 tonnes. Out of this, 56% is in the soil, 33 % in aboveground biomass, 9% in belowground biomass and 2% in litter. Average carbon density was estimated as 100 t/ha in the landscape. Mean aboveground carbon density was determined at 33.60 t/ha. In Reserved Forest the abovegroun...

Mahfouz M.M. Abd-Elgawad 

...t-align: justify;">Sound sampling of nematodes is basic to their related research and management. Several models of nematodes have been applied to characterize their possible spatial and temporal distribution patterns. These models can provide several advantages such as determining sample size optimization and nematode transformation counts necessary to meet assumptions for parametric stat...

Mahmoud M.A. Youssef1, Wafaa M.A. El-Nagdi1*, Hassan Abd- El-Khair2, Usama S. Elkelany1, Mahfouz M.M. Abd-Elgawad1 and Mona G. Dawood3

...synthesized products for safe and effective control of crop pests. This study investigated the effects of Trichoderma viride (Tv) and T. virens (Tvr) as antifungal agents individually or combined with pomegranate peel aqueous extract (PP) on M. incognita infesting potato cv. Spunta. Based on the percentages juvenile reduction in soil at harvest time, the combined treatments of Tv or Tvr with PP recorded 66.8 or 60.5% nematode reductions, respectively, as each ...

Ferry Lismanto Syaiful1*, Jaswandi Jaswandi2, Mangku Mundana2, Ilham Ilham2, Novirman Jamarun1, Efrizal Efrizal3

...bean seeds on multiple dosages in the growing seeds tests for the accuracy, sensitivity, and phases of local buffalo pregnancy, and detecting buffalo pregnancy quickly, massively, and cheaper. The object of this study was 40 local buffalo post-AI with the criteria of BCS ≥3 and 2-3 parturition. The research medium used was green bean seeds. Then use urine and water samples. The urine sa...

Hayder M. Watban1,2, Nabeel M.H. Al-Maaly2

...camels were examined and samples were collected from lungs of pneumonic camels slaughtered in Al-Muthanna abattoir. Swabs were taken from lungs of slaughter camels, and cultured on blood and MacConkey agars. The bacterial growth was subjected into gram stain and subculture to Klebsiella pneumoniae selective media. Subsequently isolated bacteria were subjected to molecular detection using conventional PCR and sequencing. The finding showed that Klebsiella pneum...

Nurul Fajrih1,2, Komang Gede Wiryawan1*, Sumiati1 and Suraya Kaffi Syahpura3

...omannan, Prebiotics, Polysaccharides, Banana corms, LAB
...

Retno Setyaningsih1, Sri Murtini2*, I Wayan Teguh Wibawan2, Surachmi Setiyaningsih2, Ekowati Handharyani3

...signs of RHDV. Samples assayed with commercial ELISA Kit using DAS Technique. The informations (age, sex, breed) of antibody titers against RHDV from previous study is used for samples profiling. The results showed all samples negative for RHDV antigen with average value ±0.04 (positive cut off >0.334). Seroc...

Roberto Gallegos-Acero1, Ali William Canaza-Cayo2,4*, Francisco Halley Rodríguez-Huanca1, Rubén Herberht Mamani-Cato3, Abigail de la Cruz1, Júlio Sílvio de Sousa Bueno Filho4 

...population, from the Quimsachata Research and Production Center of the Illpa-Puno Experimental Station of the National Institute of Agrarian Innovation, Peru. Data from 1350 Suri alpacas born from 1993 to 2015 (636 males and 714 females) were analyzed using the method of genealogical analysis method. ENDOG program v.4.8 was used for the calculation of the following parameters of the genetic structure such as: average inbreeding coefficient (F), average related...

Nurul Isnaini*, Dedes Amertaningtyas, Hanief Eko Sulistyo, Aulia Puspita Anugra Yekti, Faizal Andri

...https://odamid-apse1.oda.sas.com/SASStudio/). Comparing two results, it can be seen that the different enriched media gave gradually significant differences (p < 0.05) in the egg weight, number of eggs of BSF success to hatch, hatchability, length, width, and number of change becoming flies at period shown. Conclusively it has been shown that the different enriched media gave positive e...

Abd-Alfattah Abd-Alhamid Alderey1, Nabila Elsaid Mahmod El-Kassas1, Eman Said El-Hadad1*, Manal Hamdy Gomaa1, Eman Ashour Hussein2  

...re determined, and blood samples were taken for hematological and biochemical assays. Results showed significant (P<0.001) increase in feed intake with all SOP diets compared with controls. Feed conversion ratio was improved (P<0.05) in response to feeding hens on the diet containing SOP15%+E compared to control. Number, daily mass, and production rate of eggs were increased (P<0.001) by SOP15%+E and SOP20%+E or DY....

Ahmed Al-Hasnawi*, Wafaa Kadhim Jasim

...As azathioprine’s usage in our environment grew quickly, it became important to assess their toxicity, The goal of the current investigation was to determine if aloe vera extract had any protective effects against the hematological and histopathological alterations brought on by azathioprine. animals are evenly divided into four categories: (G1) group: were received distilled water. (G2) group: were administrating azathioprine (50mg/kg. b. w). (G3) group...

Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2

...t 5°C storage. Semen samples were collected twice a week from a 2-year-old Kacang goat using an artificial vagina. The study design consisted of three treatments; T0, T1, T2 and T3, consisting of 0%, 10%, 15%, and 20 % of sugarcane juice, respectively. The measured parameters included progressive motility, viability, and abnormalities of spermatozoa. The data obtained were analyzed using the analysis of variance (ANOVA) followed by Duncan’s multiple ...

Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail

...nd newly sequenced Oryza sativa L. cultivar Dilrosh-97 using bioinformatics approach. The possible cis-acting regulatory elements were analyzed using PlantCARE and PlantPAN3.0. Phylogenetic tree was constructed using MEGA7 and docking of regulatory elements with the corresponding Transcription factors was performed using pyDockDNA. The OsGLP12-3 promoter region (1863bp) was PCR amplified using genomic DNA from Indica rice cultivar Dilrosh-97 and subsequently p...

Abir Ishtiaq1*, Abdul Ghaffar2, Maria Younas1, Tasveer Ishtiaq1, Zara Naeem3 and Muhammad Naeem4*

...is purpose, twenty-eight samples of H. nobilis, of different body size were procured from a private fish farm, for analysis of body composition parameters’ variables in relation to body size and condition factor. Mean body weight and total length was measured and condition factor of farmed collected fish was calculated as 556.45±28.24 g, 34.78±0.60 cm and 1.30±0.02, respectively. On wet weight basis, mean percentage for water, protei...

Tanvir Hussain1,2*, Kafeel Ahmad2, Muhammad Bilal Zia1, Hammad Uddin1, Khalid Hussain1, Muhammad Inamulla Khan3 and Said Akhtar Khan1

...tes were selected and 20 samples (needles) from each site were collected. Six RAPD markers produced 92 amplicons with sizes range 250-2000bp. Among all studied populations 100% polymorphism was observed. The highest percentage of polymorphic bands (PPB=82.61%), observed number of alleles (na=1.82), effective number of alleles (Ne=1.34), Shannon index (S=0.33) and Nei,s genetic diversity ( He=0.21) were scored for ANP (Ayubia National Park) population whereas t...

Afshan1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Tahira Tayyab1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Syed Weqas Ali7 and Assad Ullah8 

... commercial corn based basal diet with different concentration of selenium powder. Basal diet (BD) was fed to Group A, which kept as negative control group and no dexamethasone (DE) was offered. Group B was considered as (+ve control group) fed with (BD+15mg DE/kg). The group C, was fed with (BD+0.2mg Se + 15mg DE/kg). Similarly, group D was fed with (BD+0.3mg Se+15mg DE). Group E, fed with (BD+0.4 Se+15mg/kg feed). At the e...

Ahmad Khan1, Muhammad Amjad Ali1, Muhammad Tahir1, Samreen Nazeer2*, Muhammad Zubair Akram3*, Muhammad Arslan Azmat1 and Sabina Asghar4

... (9.3g). Ujala-16 and Faisalabad-86 had minimum galls per pot and egg mass index of 1.6:1.2 and 1.9:1.8, respectively. Mexipak-65 had maximum 9.4 galls per pot. There were significant variations observed among the varieties, indicating varying levels of resistance or susceptibility to nematode infestation. Ujala-16 and Sehar-06 demonstrated resistance (R) having maximum RGS (Root gall Severity) of 2.2 and 3.1 with minimum RI (Resistance Index) of 34.6 and 32.5...

Ehtesham Khan, Talha Ahmed, M. Irfan Anis* and Syed Ahsan Rehman

... dataset. And also, the usage of IOT for remotely monitoring of the sensors data.

...

Hafiz Nawaz1*, Kashaf Nawaz2, Attiq ur Rehman1, Muhammad Bashir3, Mussera Hira2 and Mariyam Nawaz4

...">Pakistan grows 200 thousand hectares of barani (rain-fed) and irrigated mungbean (Vigna radiata (L.) Wilczek). 80–85% of mungbean area is in Mianwali, Bhakkar, and Layyah of Punjab. Some viral diseases cause little economic damage, while some do. Thus, mungbean viral traits, differentiating features, and dispersion must be studied critically. Mungbean Yellow Mosaic Disease (MYMD) is a whitefly-transmitted viral disea...

Fateh A. Badi*, Anwar A. G. Al-Kubati, Naif Al-Gabri 

... to reduce using un-necessary vaccination and antibiotic abused. The effects of combination of Ganoderma lucidium and Origanum vulgare on broilers chicken are not completely understood. In this study, four broiler chicken groups around 3 week age each group contain 10 chicken was divided randomly. First group was feed with (2g/ Kg feed) from Ganoderma lucidium (G group), second group was treated with ( 2g/Kg feed) Origanum vulgare (O Group) in a feed and the...

Haneen Imad Al-Sultani1*, Ahmed Obaid Hussain1, Hazar Shakir Saleh2 

...l group was given normal saline solution, the second group 10 mg/kg rosuvastatin, the third group was given 20 mg/kg rosuvastatin, while the fourth group was given 40 mg/kg rosuvastatin. The dose was continued for 60 days in a once-daily dosing regimen. After the end of the experiment, chemical analyses were performed for kidney function, including cystatin C and vitamin D3. Then, the rats were dissected, and kidney tissues were taken for histological stu...

Sajida Batool1, Mubeen Akhtar1, Shafaat Yar Khan1,2*

...ealthy females (n=60) of same age group were included in this study following Rotterdam ESHRE/ASRM criteria. The parameters evaluated were; white and red blood cells and platelets figure, lymphocytes, neutrophils, monocytes and eosinophils percentage and serum level of luteinizing hormone, follicle stimulating hormone and interleukin-3 in both groups. Two tailed, independent t-test and Pearson co-relation was applied to analyze hematological and biochemical pa...
Zahir Muhammad1, Muhammad Zubair Anjum1*, Shamim Akhter1, Muhammad Irfan1, Saira Amin1, Yousaf Jamal1, Sharjeel khalid2 and Shakira Ghazanfar2
...rum and Pediococcus pentosaceus on the growth performance of the genetically improved farmed tilapia (GIFT) (Oreochromis niloticus). A total of 120 fingerlings were acquired, assigned randomly into 4 groups (n=30/group) received one of four experimental diets each (30% crude protein supplemented with either T1-L. plantarum1×108 cfu), T2-(P. pentosaceus 1×108 cfu), T3-(L. plantarum and P. pento
Waheed Ahmad1, Muhammad Tayyab1*, Bushra Muneer2*, Abu Saeed Hashmi1, Mansurud Din Ahmad3, Shagufta Saeed1, Muhammad Nauman Aftab2, Sehrish Firyal1, Muhammad Wasim1, Muhammad Azam4, Muhammad Talha5 and Ali Raza Awan1
...re supplemented in the basal diet with 2500, 5000 and 7500 IU/kg of locally characterized thermostable protease while group E was supplemented with the commercially available neutral protease (5000 IU/kg) that served as a positive control. The trial lasted for five weeks and during the trial period birds were offered ad libitum access to water and feed. The maximal weight gain was recorded when the feed was supplemented with 7500 IU/Kg of locally characterized...

Yunxia Guo1, Xia Zhou1*, Xin Huang2 and Fagang Zhong2

...y-Bauer disc diffusion assay was applied to calculate the antimicrobial resistance profile of bacterial isolates. The survey results showed that bronchitis and bronchopneumonia were commonly occurring respiratory diseases in cattle. The highest incidence of bacterial organism Staphylococcus aureus (83.6%), followed by Streptococcus pneumoniae (80.3%) and Klebsiella pneumoniae (77.0%) were recorded in the respiratory tract sa...

Kristina Morkūnienė*, Rūta Insodaitė, Laimutis Kučinskas, Renata Bižienė 

...l study involved 117 DNA samples that were genotyped for three single nucleotide polymorphisms (SNP) using the real-time polymerase chain reaction method. Statistical analysis was performed with SPSS Statistics 20 and PLINK software. SNP at position 7454459 (Asn → Thr) of Mblk-1 gene mutant allele A was more common in untreated domestic and wild honey bees (18.90% and 8.14% respectively; p=0.001) compared to treated domestic bees which persistently infect...

Ghani Khan1, Saeed Ahmed Soomro1, Abdul Kabir2, Abdullah Iqbal3*,Naik Muhammad Marri 4, Syed Ahmad khan5, Muhammad Roidar Khan6 , Anees ur Rehman1, Muhammad Zakir Khan7 

...s a hormone that is necessary for lactation and the production of milk. It has frequently been used in the dairy sector to boost the production of animal milk. The goal of this research was to find out how oxytocin affected the physicochemical properties of buffalo milk. Milk samples were collected from thirty lactating buffaloes, divided into a control group and an oxytocin-treated group, and further subdivided by lactation...

Zain Ali1, Amjed Ali1, Bilal Ahmad Khan1*, Muhammad Ather Nadeem1, Muhammad Asif1, Adnan Ashraf1, Muhammad Ehsan Safdar1, Iram Inayat2, Aneela Nijabat3 and Rameez Hussain3,4

...ure contents of both the samples and combined silage had not much difference among them, but the protein content, fat content and the pH of the combined silage had remarkable difference among them. The Syngenta-8711 + sunflower and Monsanto-6142 + sunflower varieties were best and had maximum mean values for most of quality parameters, (Fat contents: 14.99%, moisture contents: 38.66%, protein contents: 17.41% and pH: 4.10). ...

Mukkram Ali Tahir1, Noor-us-Sabah1*, Muhammad Waryam Warraich1, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2

...pping which made it necessary to find out innovative ways to conserve native K in soil. Following study was carried out to possibly find a way to overcome bioavailable potassium losses and to access the efficacy of carbon sequestering fertilizers in this regard using wheat as a test crop under aridisols. Different carbon sequestering fertilizers (CSFs) i.e., press mud, compost and fly ash were applied @ 0% (control), 0.5 and 1% of soil weight along with recomm...

Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7

... Alternaria alternate, Fusarium solani and Aspergillus flavus were studied by Well diffusion method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoca plants have21 and 22mm MIZD. Methanol 20%has maximum antioxidant activity in O. basilicum wh...

Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2

...chondrial DNA. The blood samples from hog deer were collected and DNA was extracted by using in-house organic methods. PCR was used to amplify a total of 880 bp of overlapping genes, which were then sequenced. Bioedit software was used to align, and edit the sequences, and single nucleotide polymorphisms (SNPs) were evaluated. The MEGA X-based maximum likelihood method was employed to study the genetic relatedness between sequenced sa<...

Faiza Naeem1, Muhammad Farooq Sabar1, Muhammad Usman Ghani2,*, Qurat Ul Ain1 and Qurat-ul-Ain Zafar1

...new pathways including (hsa04658) Th1/Th2 and (hsa04659) Th17 cell differentiation pathways having STAT6, IL4, and IL13 genes having contribution in asthma. A network of appropriately matched proteins was provided by the string that was further utilized for centrality-measurement which ranked the thirteen most persuasive genes from top to bottom as IL10, IL4, ORMDL3, IL13, MS4A2, ADAM33, STAT6, GSDMA, IKZF3, LRRC3C, IL27, CD...

Shudong Liu1,2, Shuai Wang1,2, Haoran Cui1 and Genyuan Chen1,2*

...p controls were from the same livestock farm. DNA was extracted from ear tissue using the saturated phenol-chloroform method. The DNA was genotyped using the Illumina Ovine SNP50 Bead Chip. After quality control, we detected 4,8335 SNPs, which included four SNPs that are significantly associated with the ACMS of sheep. We identified four quantitative trait loci (QTL) regions for ACMS. These QTLs on Ovis aries (OAR) 2 and OAR...

Muhammad Ayub1*, Syed Arif Husain Rizvi2, Ishtiaq Hussain3, Musa Ali Hashmi4, Iqbal Hussain5, Shahid Hussain1, Zakir Hussain1 and Rehmat Kabir6 

Amna Ali Mohammed Makoof Zabanoot, Al Wafaa Ahmed Suhail Qatan, Amal Salim Mahad Hubais, Khadijah Hamid Musallam Bait Said and Selvaraju Sivamani*

...radiata) plant. The soil samples were collected from eastern (16.9931° N, 54.7028° E) and western (16.7118° N, 53.1857° E) mountainous ranges of Dhofar Governorate, Oman. Soil and rejected lime samples were evaluated for pH, electrical conductivity, particle size, moisture. In addition, the collected soil samples were mixed with various proportions of rejected lime (10 to 5...

Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2 

...urcumin phytosome. Blood samples were collected from anesthetized rats through cardiac puncture by terminating the experiment. The samples were analyzed for total cholesterol (TC), triacylglycerols (TAG), high-density lipoprotein (HDL)-c, low-density lipoprotein (LDL)-c, very low-density lipoprotein (VLDL)-c concentrations, tumor necrosis factor-alpha (TNF-), and interleukin 10 (IL-10) concentrations. The results revealed t...

Sabi Ur Rehman1*, Laiba Arshad1, Saman Ali1, Shazma Massey2, Safeer Khan3, Abdul Samad4 and Fazal-Ur-Rehman5

... method using Sarcococca saligna leaf extracts to successfully produce both silver and gold nanoparticles. The formation and stability of the nanoparticles were verified through a change in physical colour and characterized using Ultraviolet-Visible spectroscopy and Scanning Electron Microscopy. Antibacterial and antifungal activity assessments revealed moderate efficacy against Candida albicans, Staphylococcus aureus and Escherichia coli, comparable to Amoxic...

Muhammad Luqman1*, Muhammad Talha Shoaib1, Muhammad Yaseen1, Umair Safdar2 and Hassan Raza2

... method using convenient sampling was adopted for this research and data was collected from 300 respondents with the help of structured interview schedule. The results revealed that all the respondents (100%) considered ‘enhance farm production’, ‘control of insect/pest’ and ‘weed eradication’ as major factors for application of pesticides. Moreover, results also indicated that 66% of respondents were applying 2-6 and above ...

Sara H. Zughayyar*, Amer H. Gyad 

...ols, resins, flavonoids, saponins, alkaloids, coumarins, terpenes, and glycosides, while steroids and proteins were absent. The extract displayed antidiarrheal activity comparable to loperamide, showing a significant reduction in intestinal content volume and weight (P≤0.05). In conclusion, the ethanolic extract of Prosopis farcta L. fruits demonstrated promising antidiarrheal activity, potentially due to the phytochemical constituents identified, warrantin...

Rana Mahmood Ahmad*, Orooba M.S. Ibrahim 

... positive control. Blood samples were obtained using cardiac puncture at three time points during the experiment: at the beginning (zero time) and on the 12th and 21st day. These samples were collected specifically for serum collection. In the present investigation, the administration of SaZnONPs to infected rats yielded noteworthy reductions in the levels of the pro-inflammatory cytokine TNF-α. SaZnONPs demonstrated a...

Kanda Yanuar Muhamad1,3, Trioso Purnawarman2, Chaerul Basri2* 

...ng in reptile import and sales businesses all around Indonesia, utilizing the snowball sampling method. Data was collected through structured questionnaires administered during interviews. According to the research, most employees (63%) working in reptile import and sales industries have excellent knowledge about zoonoses-assosiated with imported reptiles, while about one-third (34%) have ...

Arslan Muhammad Ali Khan1, Rao Zahid Abbas1*, Zia ud Din Sindhu1, Muhammad Shahid Mahmood2

...nd the tick repellency assay. GC-FID provided that the main compounds of A. subulatum essential oil were monoterpenoids (limonene and α-terpinene, and α-terpineol) (33.8%) while other chemical compounds were α-terpinolene (9.5%), sabinene (9.3%), 1- Carveol (8.9%), α-phellandrene (7.8%), linalool (4.7%), myrtenol (4.7%), nerolidol (4.6%), β-pinene (4.6%), terphenyl acetate (3.6%), 1, 8-cineole (2...

Syed Nouman shah1, Anees Ur Rahman1, Midrar Ullah2, Shahab Ahmad Nawaz1, Muhammad Said1, Abdul Kabir1,3*, Abdul Hafeez Bukero1, Maaz Ahmad1 and Muhammad Sadiq1

...rage conditions and feed samples; well-mixed feed formulations with mycotoxin binders; diversified feed ingredients and optimized feed processing techniques can reduce mycotoxin levels in silage and their negative impacts on livestock. We conclude that a comprehensive understanding of mycotoxins in silage and the implementation of effective management strategies are essential for sustainable livestock production. Future research should focus on developing alte...

Danish Kamal2, Muhammad Abbass Khan1, Ghulam Mujtaba-Shah1, Naveed Ahmed1, Maryam Iqbal¹, Basharat Hussain Shah1 and Imtiaz Ahmed1*

...and flower diameter. Tea samples were analyzed for anatomical parameters such as epidermis cells, mesophyll cells and epidermal anatomy of leaves. The stem length, stem girth, number of leaves plant-1, leaf width, leaf length, distance between node, pedicel length and flower in tea samples ranged between 9.66-71.80cm, 1.40-2.54cm, 7.66-15.66cm, 4.83-1.66cm, 11.50 3.86cm 4.76-1.50cm, 2.03-1.06 and 4.56- 2.66cm. In anatomical ...

Agha Mushtaque Ahmed1*, Ali Zachi Abdul Qadeer Alhilifi2, Fahad Nazir Khoso1, Muhammad Ibrahim Kubar1, Tehniyat Naz Shah3 and Touseef Ahmed1

...ingredients remained the same. Sixty first instar larvae were introduced in a Petri dish to observe larval duration, larval weight, and adult fecundity. Each larva was offered 0.5 mg of diet in a plastic cup and replaced after every 24 hrs. A prominent effect of different diets on larval development was noticed. The survival percentage was 100% on D3. The difference (P<0.05) was much noticeable in early larval stages from L1 to L3. The overall larval durati...

Sidra Hafeez1, Tayyaba Sanaullah2, Hafsa Naeem3, Mah Noor Hassan4, Muttalib5, Farhana Kausar6, Muhammad Salman Hameed7*, Muhammad Anayat Ullah8, Abdul Samad9, Memoona Bashir10 and Sadaf Shabbir11

... and flavonoids and necessary for food security against disease causing agents like bacteria, fungi etc. Black rot is a serious disease in pumpkin caused by fungus (Didymella bryoniae) and damaged fruit with the symptoms of dark brown lesions but causal organism identification through DNA is still unknown. We collected infected leaf from C. pepp from the vicinity of The University of Agriculture, Fai...

Ying Chen1, Chao-Zheng Li2, Zhun Yu3, Jia Zhou4, Yan Xu4, Zhe Lin4, He Lin4* and Xiao-Wei Huang4*

Jinwen Liu, Hong Li, Jinhua Zhang, Jianping Li and Xiujuan Yan*

...tude interactions at the same time. In conclusion, it is suggested that there may be a mutual inhibition between different aboveground and below-ground herbivores when the development of corn declined. These findings will contribute to a better understanding on the interaction mechanism between above-ground and below-ground herbivore on the host plants, and have guiding significance for agricultural pest control.

...

Adeel Kazam1, Safdar Sidra2, Zulfiqar Ali1*, Rida Ahmad1,3, Ahmad Bilal1 and Aliza Batool1,3

...birds of an important Ramsar site, Uchalli Wetland Complex, along with Ahmedabad lake. Data were collected by direct (point count) as well as indirect method (interviews) from December 2020 to May 2021. In total, 139 avian species of 27,450 individuals were recorded at study sites. Results revealed that the species richness was maximum at Uchalli (133), followed by Khabbaki (92), Jahlar (88), and Ahmedabad lake (79). The Shannon Weiner index and Simpson index ...

Ismail Demircioglu1, Yasin Demiraslan2, Barıs Can Güzel3*, Ali Koçyigit4 and Aysegül Demircioglu5

...eep were used for the dorsal analysis of cranium and heads of 8 adult Hamdani and 9 adult Awassi sheep were used for lateral analysis of cranium. In the dorsal and lateral comparison of the breeds, the first principal component (PC1) explained 41.905% and 39.078% of the total shape difference, respectively. When the procrustes coordinates were examined, it was found that the samples were b...

Anas M. S. Al-Haj Afandi*, Ahmed H. F. AL-Bayati 

...hat in group (B). At the same time, the macroscopic changes in the caprine acellular dermal matrix treatment group included decreasing the thickness of a sheet and partial degradation of implants in the site of implantation as well as, thinning of white fibrous connective tissue over the sheet. depending on the clinical and macroscopical outcomes, it can be concluded that bovine tunica vaginalis sheets were better than caprine acellular dermal matrixes for rep...

Siswanto Imam Santoso*, Agus Setiadi, Maulina Syafa’ati Ningrum 

...rtnership with Ciomas Adisatwa Ltd in Kaliwungu District, Kudus Regency. This research uses a survey method. The sampling method in this study was total sampling or census with a total of 6 farmers. The sample was divided into two levels: strata I 1-30,000 broilers and strata II > 30,000 broilers chicken. The data analysis method used was descriptive ...

Khansa Jamil1, Muhammad Ramzan Khan1, Asad Jan2 and Ghulam Muhammad Ali1

...icines have proved to be safe to use. Crude extract of Caralluma tuberculata stem was used to examine its antibacterial activity and phytochemical screening. Antibacterial assay was carried out by well diffusion method. A maximum number of compounds were eluted by methanolic extract by thin-layer chromatography and a total of 70 compounds were identified by GC-MS analysis. The phytochemical screening showed that the methanol...

Muhammad Afzal1, Muhammad Ashraf Sumrah2*, Azhar Hussain2, Zoma Saleem2, Syed Hamza Mehfooz2 and Javeria Sherani3

...son of both varieties in same agro-climatic conditions reflects that Murree Gola variety of avocado may give better economical returns to the growers.

...

Nusirat Aderinsola Sadiku1, Oluwatoyin Adenike Fabiyi2* and Tesleem Taye  Bello

...not nematodes of Lactuca sativa in greenhouse trials. Bambusa vulgaris biomass was carbonised at 500 oC in a locally fabricated pyrolizer with automatic temperature control to obtain the Bamboo Pyroligneous Liquor (BPL). Two weeks old lettuce plants were inoculated with 1,000 juveniles of Meloidogyne incognita. BPL was applied at three concentrations of 100, 200 and 400 mg/ml. Results revealed that nematode infected lettuce ...

Muhammad Ali Raza1*, Aneela Zameer Durrani2, Muhammad Muddassir Ali3, Tariq Usman4, Bilques Bano5, Nazia Rubab6, Syed Tasadak Mehdi7, Muhammad Wasim Iqbal8, Kumayl Hassan Akhtar9, Hira Hameed10

...mpare and to justify the said seed consumption as an anthelmintic by the customary therapists of animals in South-Asia. Crude aqueous extract (CAE) from seeds of A. indica at the dosage of 2 g/kg of body weight,  levamisole HCL at the dose rate of 7.5 mg/kg and 3.0% oxyclozanide BP (vet), 1.5 levamisole hydrochloride BP (vet) and 0.382% cobalt sulphate in combination were administered orally to the cattle naturally infe...
Xiao-Wei Huang1,2, Mei-Li Liu1, Jin-Ji Wang1, Yue-Xin Liu3, Zhe Lin1, Chun-Shu Rong4* and Ji-Xiang Ren4*

Yu Song1*, Yueping Wei2 and Peng Wang3

...s. Therefore, it is necessary to further explore its mechanism. In this study, the samples of different growth stages of rice, including tillering stage (TS), jointing stage (JS), heading stage (HS) and mature stage (MS) were collected to analyze the impact on the community composition, diversity and functional potentialities of the rhizosphere bacterial in the coupled rice-crab system were investigated through 16S rRNA gene...
Zhi Qiang Gao*, Dan Dan Zhang, Qin Gmei Qin, Xiu Xiu Li, Li Li, Yan Zhong Xue and Shifeng Guo
...is purpose, brain tissue samples were collected from 40 patients diagnosed with glioneuronal tumors (GNTs), including 25 cases of gangliogliomas (GGs) and 15 cases of dysembryoplastic neuroepithelial tumors (DNTs). Then, the DNA of GNTs tissues was extracted, and the PSEN1 E280A mutation was detected by sequencing. Finally, the correlation of PSEN1 E280A mutation with clinical characteristics was analyzed. PSEN1 E280A mutation was detected in 16 GNTs patients ...

Ashfaq Hussain1, Javed Khan2, Ammara Blouch3, Ahmad-Ur-Rahman Saljoqi1, Ashraf Khan4,5* and Zaheer Sikandar6

...tages, while the Indispensable mortality (I.M.) was found to be maximum at the egg stage and minimum at 4th instar and pre-pupal stages. Life expectancy (ex) was found to be maximum in the egg phase (4.67) and minimum in the pupal phase (1.5). The total K value for the whole generation was 0.33.

...

Nafissa Sahel1*, Fadela Chougrani2, Abderrahim Cheriguene3 and Zineb Hamani1

...l yeasts and moulds. All samples tested for pathogenicity were negative for pathogenic germs.

...

Adnan Ibrahim Khamis1, Jasim Mohammed Saleh2*, Najwa S. Ali3 and Anwer Thamer Ghaffoori2

...ired field. The research sample included (228) respondents distributed over (11) agricultural divisions. A descriptive approach was used as a method to achieve the research goals. A questionnaire was designed to collect data for the purpose of the study. It contained two main axes. The first axis contains areas (9) for developing the skills of rural youth, paragraph (69) and the second axis contains problems and obstacles that hinder the work of agricultural p...
Naila Amjad1, Muhammad Khalid Mukhtar1*, Hafiz Azhar Ali Khan2, Shafaat Yar Khan1 and Sania Waliat1

Wei Wan1, Xiao-Li Wang1, Shu-Juan Wang2, Qin Si2 and Li-Qing Duan1*

...screen biodegradable and safe insecticides, 12 representative monoterpenoids in essential oils were evaluated for their acute toxicity, synergistic or antagonistic effects on adult P. sinica. The modified residual film method was used to test the toxicity of individual and binary mixture of these monoterpenoids against adult P. sinica. The effects of these monoterpenoids on acetylcholinesterase (AChE) and glutathione S-transferase (GST) activities in P. sinica...
Muhammad Furqan1*, Zulfiqar Ali1, Muhammad Mudassar Shahzad2, Rida Ahmad1, Faraz Akrim3, Imad-ul-Din Zangi4
...ign: justify;">Kalij pheasant Lophura leucomelanos is habitat indicator and the information about its habitat characteristics and suitability is lacking. In the current study, presence of kalij pheasant was recorded from 166 sites of Mirpur Division Azad Jammu and Kashmir, Pakistan. The maximum abundance was recorded at Gaian site (2.33/ha). Estimation of Habitat Suitability Index (HSI) from 166 sites revealed that ten sites...

Farhana Umer1, Muhammad Sheryar2 and Sangam Khalil3*

...les, principles for bird safety, photographic records, public views about deadly poles and Ultraviolet vision in Birds. This study also highlights the cause of bird casualties on high and large scale from overhead power lines. It presents different technical solutions for bird safety such as proper adjustments to the routing of the power lines and power pole constructions can effectively reduce the risks posed to birds. Deta...
Muhammad Javed Iqbal1, Farah Rauf Shakoori1*, Bushra Muneer2 and Abdul Rauf Shakoori3
...HER2 enriched and 2.5% Basal-like. Screening of mutations in PIK3CA, AKT1, MTOR and PTEN genes was carried out and analyzed by Next Generation Sequencing (NGS) using the Whole Exome Sequencing (WES) to explore possible mutations in PIK3CA, AKT1, MTOR and PTEN genes. The results revealed that there were 3 mutations namely Q546K, E545K and H1047R were present in the PIK3CA gene. AKT1gene has 2 mutations E17K, and E242 (silent mutation). MTOR gene has 2303, L2208...

Shehar Bano Bhatti1, Muhammad Irfan Malik2, Rabia Faryad Khan1, Mahmood Ul Hassan2, Muhammad Shahbaz Aslam3 and Zaigham Abbas1*

...es. Among the various causative agents of asthma, allergens and genetic susceptibility play a key role. HLA is a complex region, and most of the GWAS studies on asthma are limited to simple gene markers. This study aimed to determine the association of HLA DRB1 alleles with atopic asthma in the Pakistani population. Blood samples of 568 subjects were collected from Punjab, Pakistan. HLA typing was performed by using sequence...

Hualong Jiang1, Tao Li2,3*, Jing Xiang3, Hanqing Yang3 and Maolin He3

...te human travels and transactions. However, limited information is available on the ecological impacts of bridge constructions on local microeukaryotic compositions and functional shifts. In the present study, next-generation sequencing and bioinformatics analyses were performed to compare changes of microeukaryotic communities in local sediments influenced by sea-crossing bridge construction. Relatively low levels of alpha diversity and high levels of beta di...
Riffat Sultana1*, Mohan Lal1, Samiullah Soomro1, Santosh Kumar2 and Ahmed Ali Samejo1
...rs were collected. These samples were sorted out into 07 genera and 11 species. Morphological and morphometry account of each species with illustration ecology and global distribution was given. Taxonomic keys have been generated at generic and species level.

...

Mubashar Hussain*, Habiba Razaq, Muhammad Faheem Malik, Kiran Aftab, Jaweria Riaz and Somia Liaqat

...nd Mandi Bahauddin) were sampled by handpicking and placing pitfall traps from August 2020 to July 2021. A total of 4008 specimens were collected which belonged to 18 species, 11 genera, nine tribes, and five subfamilies. Data were analyzed with Similarities Percentage (SIMPER) analysis and diversity indices were compared among those three croplands. We found that Pheropsophus lissoderus was the most abundant and dominant species in all three study sites where...
Iko-Ojo Charity Ikwe Agada, James Agbo Ameh, Olatunde H. Olabode and Martha Echioda-Ogbole*
...urrence in Rottweiler, Alsatian and Boerboel dogs with susceptibility in male dogs (54%) than female dogs (33%) while age distribution showed high CPE affinity for younger dogs less than 12 months (86%) than older dogs (10%). In conclusion, this highlights CPV as a leading cause of canine gastroenteritis despite routine vaccinations in the study area. Further studies to evaluate field circulating CPV strains and the potency of available vaccines in Nigeria is ...
Moustafa A. Zaghloul1*, Mohamed F. Azooz1, Saleh E. Ali1*, Heba M. Soliman1, Maha M. Sayed1, Mohamed H. Kafafy2 and Alaa R. Morsy1
...which will lead to universal blueprints for multiepitope lumpy skin disease vaccine designs. A total of 40 skin nodule samples were collected from clinically affected cattle to detect LSDV using PCR targeting GPCR, RPO30, P32 and EEV glycoprotein genes. The amplified products of samples detected in the skin nodules of cattle were sequenced, and the phylogenetic tree was constructed using c...
Amany M. Reyad1*, Aya Maher Rabie1, Reda Mohamed Taha1 and Khalid El-Dougdoug2
...nt of the collected meat samples. At room temperature (24°C), the number falls over time until reaching 72 hours for all bacterial species. The results obtained showed a significant reduction in the bacterial count of E. coli, Staph. aureus
Morcos Ibrahim Yanni1* and Eid Elsaid Abdelaziz2
...or the rabies virus. The samples were tested positive by DFT and isolated on baby hamster kidney cell culture. Of them, three confirmed rabies samples were picked to determine their N and P genes by sequencing further (at the level of nucleotide and amino acid). The N and P genes in this study isolates have a great similarity to previous isolates from Egypt as well as adjacent bordering countries. At the N and P genes level,...
Akram A. Salama1, Moustafa A. Zaghloul2*, Ahmed A. Zaghawa1, Mohamed A. Nayel1, Ahmed M. Elsafey1, Mohamed F. Azooz2, Hanem S. Harb1 and Walid S. Mousa1
...rom the EEV gene, tissue samples from skin nodules (N = 40) and blood samples (N = 60) were collected from diseased animals. Sequencing and a phylogenetic tree construction were done on the amplified byproducts of samples from blood and nodules on cattle skin. By PCR technique, 55 samples out of 100 (55/100; 55%) were positive for LSDV. A representative ...
Hanaa H.A. Gomaa1*, Dalia Y.A. Amin1, Mona A. Ismail1, Basma Hamdy2, Khaled A. El-Dougdoug3
...ntial effect of nanochitosan and biomagic for treatment of the pathological effects related to virus infection in fig plants. The fig (Ficus carica L.) cv. sultani) plants were grafted by infectious blind eye from Fig latent virus (FLV) infected plants. Infected and healthy plants were foliar sprayed with nanochitosan (ChNPs), biomagic (BM) or combination (ChNPs and BM). Microtome and ultrathin sections were ca...

Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3

...of 1000-1500g and of the same age were included in this study. SVF was prepared from bilateral inguinal fat that was harvested under general anesthesia. The harvested fat was enzymatically digested with 0.1% collagenase-I. It was then centrifuged and the SVF pellet was collected. The pellet was characterized through total viable cell count, cell per gram and percentage of viability by using Neubauer’s chamber. The average cell yield per microliter was 2....

Oghenebrorhie M. Oghenochuko1,4*, Olubukola T. Adenubi3, Olusola L. Ajayi3, Fakilahyel M. Mshelbwala3, Johnny O. Olukunle3, Samson A. Rahman3 and Godfrey N.O. Ezeri2

.../l), flavonoids (0.46%), saponins (0.28%), tannins (0.95%). PCV, Hb, RBC and WBC were increased in all treatments but values were higher in bath treatment for RBC (3.0×1012/L), PCV (32.7%), Hb (10.7%). MCV, MCH and MCHC showed similar trend. Similar trends as in RBC and WBC were observed in total proteins. Liver and kidney functionality as expressed by ALT, AST, ALP, creatinine and BUN exhibited no damaging effect on organs. Degenerations were observed i...

Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1

Taidong Wang1, Xiaowei Huang1, Jian Huang2, Guangfu Lv3 and Zhe Lin1*

...ficantly inhibit lipopolysaccharide-induced fever in rats with elevated body temperature, reduce serum TNF-α, IL-1β and IL-6 levels, reduce the hypothalamus COX2, caspase3 and Bax protein expression levels and improve BCL2 protein expression levels, play an antipyretic effect.

...
Yi Zhao1,2,3, Lei Luo1,2, Liqi Tan4, Jianhua Huang1,2, Dongliang Liu1,2,5, Shigui Jiang1,2 and Lishi Yang1,2*

Rasema Majeed, Alaa Kamil Mahmood* 

...gate these effects. Chitosan nanoparticles (CNPs) can improve delivery and bioavailability of encapsulated agents such as GEE. The aims of this study to evaluate the protective effects of ginger ethanolic extract, chitosan nanoparticles, and ginger ethanolic extract loaded with chitosan nanoparticles (GEE-CNPs) against DNA damage and pancreatic histological changes in dogs with alloxan-nic...
Haigang Wu1,2, Bingjie Ma1, Jinni Liu1, Fei Zhang3, Kaiwei Deng1 and Hualing Gong1,2*
...s and the effects were dosage-dependent with an optimal dosage of 1 % w/w. 

...

Franciscus Rudi Prasetyo Hantoro1,2,*, Dwi Sunarti1, Turrini Yudiarti1, Sri Sumarsih1, Rini Nurhayati2 

... cecum, weight of the bursa fabricius and spleen, levels of malondialdehyde (MDA), number of Newcastle Disease Virus (NDV) antibodies, increase in body weight, and feed efficiency. High stocking density has a significant impact on increasing the number of LAB and coliforms in the ileum, reducing the weight of the thymus, and reducing the amount of feed consumed. The lower the crude protein level, the lower the NDV antibody titres, but it did not affect the blo...

Khalid Ali Khan1,2,3* and Hamed A. Ghramh1,2,4

...y bees including Apis dorsata, A. florea, and A. cerana whereas one exotic species A. mellifera are present in Pakistan. Honey produced in Pakistan enjoys good repute in the Middle East due to its unique taste and quality. Pakistan exports around 4,000 tons of honey with a worth of about $ 23.00 million to Arab countries every year. It is believed that the Pakistani beekeeping industry is entering an era full of opportunities.

...

Ho Thi Dung, Nguyen Van Chao, Nguyen Thi Hoa, Le Dinh Phung, Tran Thi Na, Nguyen Thi Thuy, Tran Nguyen Thao, Pham Hoang Son Hung* 

...f age, all chickens were sacrificed, and their cecum were collected for histomorphological observation by HE staining and checking the gene expression of tight junction genes by RT-qPCR. Results showed that chickens in the HT group tended to reduce mucosal length and higher lesion score in cecum (p=0.057). The mRNA expression levels of tight junction genes in the cecum revealed claudin 3 expression increased in the HS treatm...
Suneela, Sajida Mushtaq*, Sadia Maalik, Asma Waheed Qureshi and Moazama Batool
...e and Chrysomelidae were sampled from district Sialkot. Coleoptera was recorded as most abundant order with 39% relative abundance. By statistical analysis  significant difference (p-value< 0.005) was found in presence of diversity in different months of sampling. Shannon index was maximum in the month of May (H’=2.819). Canonical Corresponding Analysis (CCA) determines the impact of different environmenta...
Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1, Abdur Rahman Ansari3, Faiza Yasmeen1 and Tahira Batool3
...trict Jhang. Fresh fecal samples (n = 1800) of pigeons were gathered in plastic bags from district Jhang home fanciers and local retailers. Smearing, sedimentation, and flotation procedures were among the various parasitological methods used to analyze all of the samples that were gathered. Herein, the prevalence of coccidiosis was 70.7% and that of capillariasis was 15.7% whereas, mixed infection was 13.4%. Eggs per ...
Kanwal Nisa1, Sadia Roshan1, Shazia Shamas1,2*, Raheela Atta Mustafa3, Shamaila Irum1, Kalsoom Sughra4 and Memoona Iqbal1 
... Punjab, Pakistan. Blood samples of normal and prostate cancer males were taken from Gujrat, Punjab, Pakistan. Information including age, marital status, smoking, Prostate-specific antigen (PSA), testosterone, and any previous record was obtained with the patient’s consent. Exclusion and inclusion criteria were defined. The samples of control and p...

Wang Teng1,2,3,4, Li Chunhou1,2,3,4, Liu Yong1,2,3,4* and Zhu Ren5*

... Beibu Gulf based on the sampling and observational field data obtained from the competent authorities. Our results indicate that 1519 species were recorded, which belongs to 42 orders and 198 families. Compared with earlier studies, the species richness of fish in Beibu Gulf has decreased significantly, especially in large predatory fish and demersal fish. Now, overfishing, habitats destruction, pollution and eutrophication...
Budi Utomo1*, Amaq Fadholly2,3, Endang Tri Margawati2, Alek Ibrahim2, Ristaqul Husna Belgania1, Muhammad Fajar Amrullah1, Rimayanti1 and Hermin Ratnani1
... (Y chromosome). The DNA samples used for this study were 10 frozen straw of Limousin cattle and 10 samples of Madrasin cattle. The method used in this research was a descriptive analysis by duplex polymerase chain reaction and analyzing the results of the SRY gene sequencing of limousine cattle and Madrasin cattle. In the present study, we showed that the SRY gene of Madrasin cattle and Limousine cattle could be amplified w...

Abroo Fatima Qazi* and Din Muhammad Shaikh

...iabetic effects of polyunsaturated Omega-3-fatty in streptozocin (65 mg/kg body weight) induced diabetic Wistar rats. Animal were assorted to five groups: negative control group A and positive control group B and experimental groups C, D and E. Experimental groups C, D and E received Omega-3-fatty acid supplemented food in 0.3g, 0.4g and 0.5g/kg bodyweight dosage for 12 weeks, respectively. These Omega-3-fatty acids treated ...
Sudhan Chandran1*, GB Sreekanth2, Geetanjali Deshmukhe1 and  Ashok Kumar Jaiswar1
...ove fishes, viz., Escualosa thoracata (10.1 cm) and Bregmaceros mcclellandi (10.5 cm).

...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
... of sodium such as table salt, breads and rolls, sandwiches, soups, cold cuts and cured meats, savory snacks, chicken etc. However, the excessive intake of sodium may cause health issues such as hypertension, brain strokes, renal irregularity, heart attack etc. Various analytical techniques including atomic absorption spectroscopy, chromatography methods, ion selective electrode method, la...

Roheela Yasmeen1,3* , Faheem Hafeez1 , Umme Ammara1 , Rubab Younas1 , Sibtain Ahmad2 , Zulfiqar Ali3, Zaheer Ahmad Nasir4 

...m the poultry farms. Air samples both from indoor and outdoor along with the litter and feed samples of ten poultry houses were collected from the outskirts of Lahore, Punjab, Pakistan. Poultry farms were varied in feed and grouped into three categories: Group A (using Feed A), Group B (using Feed B) and Group C (using Feed C). All collected samples were digested with aqua regia and analy...

Rahmat Elahi1, Gulnaz Parveen1*, Nazara1, Faryal Ali2, Nain Tara3 and Saba Iqbal1

... seed. It appears that Fusarium semitectum was found in 13.33% of untreated seeds, while Alternaria solani accounted for 6.66%. However, in the treated varieties, both of these fungi did not appear. Aspergillus niger, on the other hand, had the highest colonies in both treated (46.64%) and untreated (56.66%) seeds. Additionally, Fusarium oxysporum was present in 16.66% of treated seeds and 49.98% of untreated seeds, while Fu...

M. Azalou1,3*, C. C. Kpomasse1, A. S. Assani3,4, I. T. Alkoiret3,4, Wéré Pitala1,2  

... farmers face almost the same difficulties, such as diseases, mortality, theft, and predators. Goose farming in northern Benin was therefore family-based, with a diversity in the activities of the goose farmers, the type of farming, and the diet. The results of this study will contribute to the implementation of programs to improve goose farming in Benin. 

...

Rana Mahmood Ahmad*, Orooba Mohammed Maeed  Ibrahim

... extracts are non-toxic, safe, and have no harmful side effects. Available in abundance, researchers have turned their attention to using these plants as anti-inflammatory. This study was designed to highlight and compare the effects of Syzygium aromaticum extracts oil and Piroxicam on inflammation circumstances. Extraction of clove bud with petroleum ether revealed a bright yellow extract with a typical clove oil smell and bud. The gas chromatography-mass spe...

Montesqrit Montesqrit1*, Harnentis Harnentis1, Sepri Reski1, Fadhli Fajri2 

...h three treatments: A (basal diet + Zinc bacitracin), B (basal diet + 1% ROSE), and C (basal diet + 2% ROSE). Each treatment was replicated six times. The observed variables were the Escherichia coli count of the intestine and the performance of laying quails (feed consumption, egg production, egg weight, egg mass, and feed conversion). The results showed that the inclusion of ROSE in the ...

Turrini Yudiarti*, Sugiharto Sugiharto, Endang Widiastuti, Hanny Indrat Wahyuni, Tri Agus Sartono, Maulana Hamonangan Nasution

...A). These included T0 (basal diet/control); T1 (basal diet supplemented with 0.5% FA); and T2 (basal diet supplemented with 1% FA). Each treatment was repeated five times with a total of ten chiks each pen. Feed additive was provided to chicks beginning 8 days after rearing and was mixed in with the basal diet. Water was available at all times. The data ...

Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2 

...CPNPs. We analyzed blood samples of malondialdehyde (MDA), total antioxidant capacity(TAO-C), protein carbonyl(PC), reactive oxygen species (ROS), and gamma-glutamyl transferase concentration(GGT). The results indicated that 200 mg/kg of SiO2 - NPs orally for 4 weeks contributed to a substantial drop in serum TAC-O, an increase in MDA, GGT, PC, and ROS concentration, and attenuation of silica’s oxidative stress status, CP NPs (T3) or SiO2 NPs (T2) admini...

Maria Endo Mahata1*, Yose Rizal1, Zurmiati1, Sepri Reski1, Indah Fitri Sakinah Limbong2, Dian Saputri2 

Omaima Khamiss
...nism of infection in the same host multiple nucleopolyhedroviruses (MNPVs) Spli MNPV, and granulovirus SpliGV. Mixed infection of the tow viruses was carried out in vivo and in vitro, showing clearly that the mixture with Spli GV enhance the effect of Spli NPV when it was introduced 24hr prior to NPV or simultaneously: In addition to' protein profiles by SDS-PAGE, TEM electron microscopy ultrathin sections and hybridization dot blots, gel transfer to follow th...
El-Dougdoug. K.A.1, Mervat, M. Fath Alah2, Reham A. Hassen3,Rehab A . Dawoud2
...derma, Aspergillus and Fusarium as contaminants microbial of potato micropropagative in vitro. The occurrence of induced resistance and increased potato plantlets were found by MS medium treated with culture filtrates based on reduction of PVY infection, disease severity, biochemical changes accompanied to induced resistance (the high level of endogenous salicylic acid, protein content, chlorophyll contents and peroxidase an...
Mohga A. El-Tahlawey1, Samah. A. Mokbel1, A.M. Mandour1,and H. A. Mohamed2
... plants feature severe mosaic on lentil cv. Giza51. mottling on cv. Giza9. mild mottle on cv. Giza4, yellow mild mottling on cv. Giza370, Seed transmission tests Of PMoV confirmed of the virus transmission through Lentil seeds. Peanut mottle virus was de...

Khalid Alhudaib

...nce. A total of 166 leaf samples (65 Apricots and 101 Peach) were collected and transferred to the Lab. to be analyzed for the causal virus. Double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) was performed using the commercially available for PNRSV. Result showed ...
Elamien***, Mohammed; Kotob**, Nahla S.; Elsayed*, Eltayeb A.; Ragab, Yasser M. and Mourad, Fathia E.
...lities. Fifty seven bOPV samples and fifty seven topV samples were taken during NIDs campaign in February 2010 and June 2010 respectively, and their potency were tested by the determination of a cell culture infective dose 50% (CCID50) in Hep-2 Cincinnati cell line and using the Karber's Formula. All topV (type 1, 2 and 3) samples were found to be potent, within World Health Organization (...

Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni

...fy;">Ninety-two positive samples for anti-HCV antibodies were selected from out patients of Center of Cardiac and Digestive System, Sohag, Egypt aged from 20 to 55 years. Then, confirmed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) for HCV. The confirmed patients were received 12 vials of BEG FN- 2a for 12 weeks (1801U/ml weekly) plus ribavirin (1000 mg for 75 Kg or 1200 mg for > 75Kg- Roche) and follow up by RT-PCR. The results showed that 6...

Elkalamawyl, I.M.; Elhddadl, S.; Swelim2, M.A.; Hamdy2, S.M. and Fahmy3, Hanan A.•

...y sensitive diagnostic assays can be achieved for detection of HBV in clinical specimens. Control of these EBV mutants, which will require new drugs, vaccines, and treatment strategies, will become the next major challenge on the path to eventual elimination of HBV infection. Keywords: HBsAg, PCR, Sequencing, Phylogenetic.

...

Ibrahim l , Madiha Salah and Ikuta 2, Kazuyoshi

...etected H5N1 in clinical samples derived from different organs including lung, liver and brain tissues with high sensitivity and specificity. The sensitivity and specificity were 71% and 100% for Sysmex avian influenza kit and 86% and 33% for Sysmex influenza MB kit. Thus, Sysmex avian influenza kit was shown to be a potentially useful tool for the direct and rapid detection of H5N1 in clinical specimens due to its high specificity. Additionally, an advantage ...

Yousifl ,3 , Ausama A. and Al-Naeem , Abdelmohsen A.

...d using a modified PCR assay. Our data explains some of the variation obtained with restriction analysis and underlines the need for a review of some of the vaccines used to control CMLV.

...
Hassanein, Suzan A.; Abd El-Wahab, Wafaa; Eweis, Moustafa and Mahmoud, Mervat M.
... In this study 465 serum samples were collected from 3 Nile delta governorates (Behaira, Mounofya and Kafer El-Sheikh) during 2009. The samples were used for detection of FMD antibodies to 3ABC non-structural proteins using commercial ELISA kit (Priocheck). The overall percentage of positive was 38.9 %. The higher percentage of positive detected in Behai...

El-Kenawy, A. A. and El-Tholoth, M. S.

...(LSDV) was detected in 23sampIes collected from clinically diseased and slaughtered cattle showed clinical signs believed to be LSD. These samples include 7 skin lesions and 16 internal organs (lymph nodes (6), lung (4), kidney (3) and liver (3)). Hyperimmune serum was prepared against reference LSDV (Ismailyia88 strain). Immunofluorescence (IF), immunoperoxidase (IP) techniques and polymerase chain reaction (PCR) were used ...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M. 
...PCRs appeared within the same specificity for H5 gene detection, while they showed difference in sensitivity as the one step conventional RT-PCR showed to be more sensitive than two steps conventional RT-PCR by 10 folds, one step RRT-PCR TaqMan probe was more sensitive than two steps RRT-PCR TaqMan probe by 10 folds, two steps RRT-PCR hybridization probe is more sensitive than two steps RRT-PCR TaqMan probe by 100 folds, finally two step RRT-PCR using hyperidi...

Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.

...io-check) for l065 serum samples were collected (735) from Sharkia and (330) from Kafr ElSheikh Governorates during 2009 from cattle and buffaloes. The higher percentage of positive was detected in Sharkia (31.7%) while Kafr Elsheikh was (27.3%). The positive result of detection of antibodies against non structured proteins of FMDV indicates that these samples come from natural infected animals. The further work is required ...

Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.

...w (2010) the tested swab samples were inoculated into the allantoic cavity of 911-day-old SPF embryonated chicken eggs (ECE) for virus isolation. The allantoic fluids (AF) of the inoculated ECE were examined for detection of avian influenza (Al) isolates using rapid haemagglutination test (HA). It was found that, all the inoculated isolates caused high mortalities up to 100% for the embryos 24-48hrs post inoculation and gave very strong RBCs agglutination. The...

Madbouly*, H.M.; Saif**, M.A. and Hussein* , A.S.

... by SIC injection. Serum samples at 10, 14, 17, 21, 28, 35, and 42 of age for HI test against NDV. Heparinized blood samples at 10, 14, 17, 21, days of age for phagocytic activity of macrophages. All groups were challenged with vvNDV for detecting the protection percent. From this study it could be concluded that MDV vaccine has an immunosuppressive effect on chicks and this could be antagonized by immunostimulant as curcuma...

Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.

...ovirus, which causes a disabling febrile infection of cattle and water buffalo. A sever outbreak was recorded in Egypt during 2000 and 2001. Using RT PCR, a primer designed to amplify 438 nucleotide fragments from the glycoprotein G gene allowed specific amplification of BEFV-cell culture isolates from Egypt (Menoufia and Alexandria Governorates) and Japan. The PCR product was sequenced and analyzed. PCR product of Alexandria isolate was cloned and labeled wit...

Madboulyl , H. M.; Tamami , S.M.; Abd Elmoneml , A.S.; Husseinl , A.S. and Arafa2, A.M.

...isolated from 5 out of 8 samples of nasal discharge and 10% homogenates of nasal sinuses collected from young turkey poults of unvaccinated flocks by passage on SPF-ECE of 5-7 days old embryos via yolk sac route. Characterization of isolated virus was done by using RT-PCR where the 5 isolates were positive for APV and ...

Abd El-Razakl, A.G. and AboElkhair2, M.

...nitored via serology, bursal indices and effect of the two vaccines on immune response to Newcastle disease and avian influenza vaccines. It was also verified by an experimental very virulent IBD challenge performed at the age of 2e day in birds transferred from the farms with appropriate control groups in a laboratory. The immune response on farm A showing higher serological titer at the age of 35 day via ELIS...

Bakr3 *, Youssef M.; Nour El-Din3, Hanan A.; Abd EL-Wahed2, Waiel F. and Hemeidal , Aläa A.

...b> and Immuno dot-blot assay. The obtained antibodies were positively reacted against PLRV infected potato tissues.

...

El-Bramawyl , M . A. S. A. and El-Beshehy2, E. K. F.'

... justify;">Bean yellow mosaic virus (BYMV) is one of a devastating pathogen that reduces faba bean crop production in Ismailia. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective BYMV disease control strategies will come from a better understanding of inheritance the resistance to BYMV disease in the crop considering. In this article we consider the determination role of the inheritance mode of the resistance to BYMV in ...

Elbeshehy, E. K.F. and Sallaml, A.A.A.

...gn: justify;">Cucumber mosaic virus (CMV) was isolated from naturally infected cucumber plants Cucumis sativus L. grown in various garden and greenhouses of Ismailia Governorate, Egypt exhibiting systemic mosaic, blistering, fruit malformation and stunted plant growth and identified by biological, serological and molecular analysis. The isolated virus gave positive reaction with CMV antise...

El-Dougdoug2, Kh.A; Ghalyl, M.F. and Tahal , M.A.

... to control a Cucumber mosaic virus (CMV). In the 1st experiment, One half of leaves of Chenopodium amaranticolor were treated with culture filtrate (CF) followed by CMV inoculation on both halves. In the 2nd experiment, The first pair of Cucumis sativus leaves were teated CF with CMV mechanically inoculated onto one leaf, the other non-treated leaf was CMV inoculated after 7 days of treatment. In 1 st experiment, CF treatme...

Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•

...plant against Cucumber mosaic cucumovirus (CMV), salicylic acid (SA) activities were determined in the inoculated and non-inoculated tomato plants. Identify of CMV was confirmed by some differential hosts and dot blot immunoassay (DBIA) using polyclonal antibody specific CMV. Water extracts of Mirabilis jalapa, Clerode...
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
... and identified from Chrysanthemum cultivation in Egypt. Both biological, serological and sequence analysis of the coat protein gene demonstrated that the virus represents an isolate of the Tobamoviridae Family. The isolated virus was nominated as TMV Chrysanthemum Egyptian isolate (TMV-Ch-EG). This virus isolate caused severe disease symptoms in Chrysanthemum plants with mo

Aboud*, K. A; Gomaa**, Hanaa H. A.; El—Taholowy***, Mohage, A. and El - Sugher**, S.

...derived from healthy and sanitary (10 mg/L thiouracil) plants showed close similarity within the first group while sanitary (20 mg/L) second group and sanitary (30 and 40 mg/L) plants third group showed 70 and35% similarity with first group. The results demonstrated that application of 10 to 20 mg/L thiouracil in vitro gave survival plantlets higher than30 and 40 mg/L thiouracil.

...

Soliman*, Ahmed M.; Mahmoud**, Sabry Y. M. and Dawood*, Rehab A.

...-align: justify;">Garlic samples showing symptoms of onion yellow dwarf virus (OYDV) were obtained from previous study and tested by indirect-enzyme linked immunosorbent assay (I-ELISA), transmitted to Chenopodium amaranticolor and then confirmed by immunosorbent electron microscopy (ISEM) for the presence of OYDV. PCR primers were used to ampli$' about ...

Eisa*, Nawal A.; Abd El-Ghafar **, N. Y. Abd EL-Mageed*, M.H.; Mohamed* , F.G. and Hasan*, Eman O.

...onas vesicatoria (the causal agent of the bacterial spot disease of tomato) were isolated from infected leaves of tomato and from tomato rhizosphere soil, using enrichment technique. The phages produced plaques (4-5mm, diameter) with a distinct translucent spreading halo. Presumptive phage particles associated with X vesicatoria were observed by Transmission Electron Microscope (TEM). Particle size and morphology of each phage isolate were examined by electron...

Azzami, Mohamed 1.; Hakiml , Aya Salah El-Din; Othman2, Badawi A. and 2 El-Dougoug, Khaled A.

... at 2007-2009. The water samples were ultrafiltered to concentrate coliphages, enteric and H5Nl viruses. Coliphages infecting Escherichia coli were detected in both of sewage polluted samples and chlorinated water samples especially in warmer seasons, however, maximum counts were recorded during summer and the minimal were detected in winter. The viruses obtained by ultrafiltration process...

Sofyl *, A.R.; Soliman , A.M.; Mousal, A.A. and El-Dougdoug , Kh.A.

...xia (CVd-IIa) and the causal agents of severe (CVd-IIb, CVd-IIc), more moderate (Ca903) and mild (Ca909).
...
...s studied. We designed a satellite map for the gummy bark disease distribution all over the world using the previous registered results. Superimposed maps of BIOCLnvf annual Mintemperature, Max-Temperature and the points distribution, indicated that gummy bark disease naturally occurs in the low temperature zones range from 8—180C at winter and from 27-380C at summer season where the altitude ranged from -351 to 1320 m. A novel method called maximum entr...

El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.

...ext-align: justify;">Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified by polymerase chain reaction (PCR) using Pospiviroid-CCR specific primers. Purified gel RT-PCR product (⁓199) was cloned into the PCR Il TOPOvect...

Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.

... that the tissue print assays allowed clear discrimination between healthy and viroid-infected grapevine plants than dot-blot hybridization, the number Of HSVd-infected grapevine plants were 10 plants, PSTVd were 10 plants and the CEVd was detected with high incidence level in grapevine, where 12 out of 100 grapevine trees analyzed were infected. The frequency of HSVd, CEVd and PSTVd naturally infected grapevine trees recorded different percentages as in singl...

Muhammad Ramzan1*, Unsar Naeem-Ullah2, Shafia Saba2, Madiha Mobeen Khan3, Naeem Arshad Maan3, Asad-ur-Rehman4, Mahreen Khalid5, Syed Waqar Hussain Shah6 and Wardah Muzaffar7

...orn is present on the dorsal side of the 8th abdominal part on each eruciform larva. The average length and width of male cocoon was 9.90 ± 1.00 and 4.74 ± 0.52 mm, respectively. The length of wingspan of male and female was 17.44 ± 1.56, 5.66 ± 0.49 mm, respectively, while body length of male and female was 8.84 ± 0.90mm, respectively.

...

Iftikhar Alam1*, Sumbal Ali1, Anwar Ali Shad2, Aneela Tahmeed1, Hafsa Nawaz1, Shumail Bakht1 and Muhammad Farooq3*

... These results suggest a satisfactory reliability co-efficient of the scale. Based on I-CVI, S-CVI and Cronbach’s alpha values, the scale that was developed and validated in the present study for the assessment of literary quality of the health story is a reliable scale.

...

Abo Elmagd l , Enas K.; Abdel-Wahab l , Kouka S.; Alrasheedy l , Zeinab E. and Khalifa2, Ahmed S.

...nonuclear cell (PBMNC) lysates, antibodies to HCV core, NS3, NS4 and NS5 epitopes by enzyme linked immunoassay (ELISA) in mothers and their infants using serum samples for both mothers and infants plus saliva for infants, in addition to detection of serum HCV- RNA (viremia) by revers...

Abo-Elmagdl , Enas K;El-Mougy2, Hala M. T. and Abd El-Salam 3, Manal

...tion of RSV antigen in nasal wash specimens collected from Egyptian infants and young children with the detection of RSV-RNA by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequency of RSV infection in Egyptian infants and young children during the winter season 2009/2010 and the effect of certain risk factors including age and gender on the extent and impact of RSV infection. The study included 72 infants and young chil...
Abdel Hamidl , Saiwa El-s.; Abdel-Wahab l , Kouka S.E. ; salehl , Laila H.;
Davis3, E. Graham ; Hastie4, Ian and Booth2 ,James C.
 
... (0.0%) in London. Urine samples from 8.6% of Egyptian children aged 2 weeks to 5 years were positive for HCMV by culture, compared with 3.6% aged 6-10 years and 5.8% aged 11-15 years. In children from London, HCMV was isolated from the urines of 11% aged 2 weeks to 5 years, 5.2% of those aged 6-10 years but from none aged 11-15 years.

...

SHARAW11'2*S S. S. A.; AL-HOFUFY2, A. N. and AL-BLOW12, M. H.

...ic acid (NA) in clinical samples for diagnostic purposes. In disease management contexts, until now, a little have been described for the molecular detection of camel pox virus (CPV). This study reports the development of a Real-time polymerase chain reaction (RT-PCR) for detection of CPV using SYBR green I chemistry. A total of 15 specimens from camels suspected of being infected with CPV were collected from Riyadh Province during 2009 and submitted for virol...

Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.

...tify;">Broad bean true mosaic virus was purified from infected faba bean plants. The UV absorption spectrum had a maximum at 260 nm and a minimum at 240m. The ratios of Amax /A min and A 260/A 280 were 1.48 and 1.71, respectively. The yield of purified BBTMV preparation was about 1.57 mg/Kg infected leaves. Production of specific antiserum against BBTMV was obtained by rabbit immunization using three different methods of injections. The titer Ofthe prepared an...

El.-Kadyl, M.A.S; Badr2, A.B.; zein3, Saiwa N. and Khalifa4, M.A.A.

...oxy benzoic acid has the same effect (80% reduction) at concentration of 0.01% and 0.1, respectively in the treatments inoculated one hr. after sprays. Six different chemical compounds applied as foliar sprays with two concentrations for each were used to study their effect on the percentage of virus infection in pepper treated plants: Actellic application gave superior results (46.7%) in decreasing the infection percentage followed by either Sumithion or K.Z....

Ahmed, AmalA.; Zein, Salwa N. and Khatab, Eman A. H.

...lign: justify;">Celery mosaic Potyviruses (CeMV) was detected and isolated from naturally infected celery plants showing mosaic symptoms suspected to be caused by viral infection, also, it was isolated from other hosts. The isolated virus was biologically purified from single local lesions formed on Chenopodium amaranticolor. CeMV was identified by its host range, symptom expression, modes of transmission and particle morpho...

Zein Salwa N; Abd El-khalik, Samaa; Khatab  , Eman A.A.H and Azzam4,Clara R.

...irst record of Tobacco mosaic Tobamovirus (TMV-S) on sunflower in Egypt. It was originally isolated from naturally infected sunflower plants growing in Giza Res. Station, showing systemic mosaic and spots. Purified TMV-S migrated as a single zone in density gradient column. Ultraviolet absorbance ofTMV-S was typical of nucleoprotein with minimum and maximum at 247 and 260 nm respectively. The ratios of A260/280 and Amax/min ...

Alhudaib, Khalid

... affected by fig plant mosaic diseases (Fig leaf mottle-associated virus, Fig mosaic virus). The main symptoms are chlorotic mottling, blotching and various types of leaf deformation. Samples were collected, with consideration of the economically importance and distribution of the cultivars, from different areas of Hofuf Saudi Arabia. Each sample was consisted of 10-15 leaves. Samples were...

*El-Helaly, Sahar H.; ** Ahmed, Amal A.; * Awad, M.A. and ** Soliman, A.M.'

...ign: justify;">Alfalfa mosaic virus (AMV) was isolated from naturally infected potato (Solanum tuberosum, L. cv. Spunta) plants, showing bright yellow blotching or mottling «Calico» and aucuba symptoms suspected to be due to virus infection. Leaf samples were collected from different localities in Minufyia Governorate, Egypt. AMV was readily mechanically inoculated by sap extra...

Ahmed, Amal A. and Fath-Allah, Mervat M.

...gn: justify;">Cucumber mosaic virus(CMV) and Plum pox virus(PPV) (Sharka) were isolated from naturally infected apricot (Prunus armeniaca cv. ElAmar) trees showing leaf mottling, marginal leaf necrosis deformation and abortive flowers grown at Kalubia and el-Fayoum Governorates, Egypt. The identities of the two viruses were confirmed serologically using DASELISA. Graft inoculation of disea...

El-Sayed • l , Eman H; Mahfouze , Sherin A.; Shaltout ,A. D.; El-Dougdoug3 ,Kh. A. and Sayed1 , R. A.

...inst BMV isolate. In the same time, it is not BBTV resistance banana plants

...

Karl Maramorosch

... viruses have failed. Causative agents of yellows-type plant diseases were classified as viruses because no fungi or bacteria could be detected in diseased plants. The inadequate characterization of viruses delayed the discovery of the pathogens of yellows-type diseases by forty years. In 1967 Japanese plant pathologists and entomologists announced the discovery of mycoplasma-resembling pathogens in diseased plants and insect vectors and the temporary recovery...

El-Tabakh, SAA l ; Abdel Wahab, KSE; Badr, AF   and Helal, IG  

...rnatant fluids (SF) were sampled before complete change with fresh MM at weekly intervals for periods extended to one month after infection. Three SF samples taken 5 days apart after HCV infection showed that detection of HCV-RNA in SF was intermittent but' detection of new native protein as well as glycopeptides was consistent.  
...
Mazharul A. M. 1, Md. S. Sarwar ,M. Nurullah 2, Keshab K. Adhikary I and M. Rahmatullah 1 
 
...al fevers. Stemona tuberosa was used in case of measles. Moringa oleifera and Azadirachta indica were used to treat chicken pox. Three plants, namely Cyathula prostrata, Amaranthus spinosus. and Calotropis procera were used to treat patients with rabies.
...

sans-serif; font-size: 14px; background-color: rgb(255, 255, 255);">Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi

...resent study, 9922 serum samples were collected from vaccinated chickens including some broiler breeders and commercial layers from the period Of December 2006 to February 2008 and tested for the immune response to the multiple vaccination with different H5Nl and H5N2 avian influenza vaccines (Reassortant H5N1 and 6 types of H5N2 vaccines) by hemaggtutination inhibition (HI) test using heterologous H5 antigen which represent non of the vaccine antigens. The

Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty

...rds 5, abattoir 3, shops sales live birds l, migratory bird l) from February to June 2006. The clinical picture of the disease were observed in infected birds that reared in farms and those of backyards (most of domestic birds) , or sold in shops (sells broilers or parrots The number of dead and culled birds in this outbreak was 34.4 millions with economical losses 2-3 billion dollars.

...

Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty

...ng January 2007 from the same locality. The strains isolated from infected chicken during 2006 were closely related to A/ty/Turkey/l/05 strain which had been isolated from turkeys in Turkey 2005 and to MVietnam/1194/04 strain isolated from human cases of Al H5Nl and induced higher case fatality in human in Viet Nam.   phylogenetic analysis of haemagglutinin (HA) and neuraminidase (NA). genes of 2006 strains revealed that these isolates are located in...

Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman

...der, where the allantoic sac route is considered the best site for virus inoculation and the virus was completely inactivated using formalin in a final concentration Of 0.1% for 18 hours at 25c0. Evaluation of the prepared vaccine batch based mainly upon vaccination of different groups of specific pathogen free (SPF) chickens using different doses and estimation of the humoral immune response using the hemagglutination inhibition (HI) test. The vaccine provide...

Arafa, A.; Kanawaty, Z.; Abdclwhab. E. M.; Hassan, M.K.; and Aly M.M.

...igratory ducks in El-Abassa Lake at El-Sharkia Governorate in 2007. To avoid repeating the scenario of H5N1 introduction to Egypt through wild migratory ducks to Egyptian poultry sectors, this prompted us to survey avian influenza in Egyptian wetlands, especially El-Abassa Lake and neighboring areas. Eleven villages around the lake were included in the surveillance. Serological examination using Avian Influenza type (A) anti...

Ahmed Abd El Samie Hassan Ali

...dom 42 field pooled calf samples were collected and inoculated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits were observed for 25 day, slaughtered then tissue harvests from spleen, lung, lymph nodes and intestine were pooled, homogenized; the supernatant was clarified and concentrated for second inoculation. A another 16 BVDV seronegative New Zeeland rabbits, classified into 4 gr...

El-Tarabili M. M., El-Shahidy M. S., Rifaat M. M., Kania S. A. and Abdelwahab Shahira A.

...tudy two real time PCR assays had been used to detect the MCF virus strains in both cattle and deer. The virus was detected in cattle samples by Ovine MCF RT-PCR while was detected in deer samples by a newly developed assay for deer MCF. Prevalence of ovine MCF was 18.5% in cattle and 58% in sheep. Nucleotide sequencing analysis of selected cases from ca...

Hassanein, S.A.*; Ibrahim, A.K**; Mervat, M.M.* and   Nahed,K.A.*

...ontrary, RFLP and RAPD assays could be used to differentiate the virus. Thus, both techniques can be applied for the molecular epidemiology of BHV -l.

...

JEHAN A. M. GAFER.' HUSSEIN, H.A. and REDA I.M.

...t-align: justify;">225 nasal swab samples were collected from diseased sheep and goats for the isolation of PI-3 virus. The samples were taken from diseased animal at seven different Governorates Kaffer el sheikh, Alexandria, EL Behaira, Port Said, Demiatta El-Qalubia and Giza through the winter seasons of years 1999 to 2003. The virus was successfully isolated from three ovine

Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.

...A rotaviruses from fecal samples collected from camel farms suffering from diarrhea in Alexandria and Esmalia governorates. After preparation of fecal samples and inoculation on MA 104 cell line for five passages, eight isolates were successfully isolated with a clear and reproducible CPE on the inoculated cells. The isolates were identified antigenically using VP6 monoclonal antibodies (M...

El-Sabagh, I.M.; Hussein, H.A.; Amer, H.M.; El-Sanousi, A.A.; Reda,I.M. and M.A. Shalaby

...d by immunoflurescence assay. Using solid phase ELISA, the VP7 expressed protein was detected intracellulary and extracellulary at 48 and 96 hours post-inoculation using polyclonal antibodies against BRV, respectively. The VP7 expressed protein was not detected in Coomassie blue stained SDSPAGE but produced a detectable band in Western blot assay. The re...

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

... A modified colony PCR assay was utilized to identify the positive bacterial colonies that harbor the recombinant plasmids carrying N gene in correct orientation. Generation of recombinant baculoviruses was achieved by co-transfection of Spodopetra frugiperda (Sf-9) insect cells with a linearized replication-defective baculovirus DNA (Bac-N-BlueTM) and the transfer vector. The recombinant baculoviruses were plaque purified; verified for the presence of target ...

*Hanan M.M. Elzahed, Abd EL-Moneim A.A, *Afaf A. Khedr, *Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I.

...in progeny chicks were assayed by ELISA. Vaccinated dams showed a high level of antibody -to (CAV) followed by moderate level for up to 50 weeks of age. In addition progeny chicks showed a detectable level Of maternally-derived antibody. New castle Disease Virus (NDV) antibodies were monitored in adult hens during the first 22 weeks of their age using Haemagglutination inhibition (HI) tech...
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
...y propagated on the yolk sac of 5-7 days of SPF embryonated chicken eggs. The virus was harvested 48-72 hours post inoculation. The titre of the virus reached 1062 EID50ml. One hundred and fifty chickens were divided into 3 equal groups. The first group was vaccinated with the prepared SHS vaccine, 2nd group two was vaccinated with imported SHS vaccine, while the third group was kept as non-vaccinated control. Evaluation of the prepared vaccine was done by mea...

Manva F.M. Abdel Monem; Gihan K. Mohammed;** Sami A.M.; and Saber M.S.*

... log10 TCID5Janimal. The safety and potency of the vaccine was followed up as well as the duration of the immunity by ELISA and SNT through 12 months post vaccination. There was no evidence of untoward effects except slight elevation of body temperature in the calves through 14 days post vaccination. The virus was recovered from the sera of 3 calves on the 2nd  and 4th day post vaccin...

sans-serif; font-size: 14px; background-color: rgb(255, 255, 255);">Sonia, A.M.; El. Sanousi, A.A.; Saber, M.M.; Daoud, A.M.; Samira, E.K.; and Ismail I.

...> 206, Quil A saponine, Ginseng extract and Montanide IMS 3015. These vaccines were compared with Al (OH) 3 gel vaccine and tested in calves. The obtained results revealed that the duration of immunity elicited by gel FMD vaccine was shorter than oil adjuvanted FMD vaccines. Results also indicated that vaccine emulsified with Montanide IMS 3015 could elicit the best protection capability with long lasting immune response (up to 42 week) in calves, i...

Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy

...meral fever (BEF). Blood samples on anti-coagulant, nasal swabs and tissues (bovine liver, kidney, lymph nodes and lung) for BEFV isolation and propagation on Vero, BHK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosi...
Ashraf M. Metwally*, Ausama A. Yousif*k , Iman B. Walaa A.   Attia M. Samy * , and Ismail M. Reda *
 
... justify;">Infectious bursal disease (IBD) serotype 1 viruses continue to cause major economic losses in the Egyptian poultry industry despite the implementation of intensive vaccination programs. A recent increase in IBD related mortality in vaccinated farms prompted this investigation into the genetic character of the circulating IBD virus (IBDV). Bursa and proventriculus samples were RT...
Eman, M. M. Soliman*; Taha, M. M.*;El-Sanousi A,;Shalaby M. S. Wasscl , Youscf Adel*
...proved to be sterile and safe when inoculated subcutaneously (S/C) and intraperitoneally(I/P) into mice (3-5 days old) and lambs without showing adverse post vaccinal reactions. Duration of immunity to RVF virus in vaccinated sheep has been determined by using both serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA) on sera coll...

Laila,A. Sedeek; Fatma,S. Mohamed and Manal Abo El-Yazyed

...ole experiment period. A satisfactory humoral immune response to both virus vaccines in vaccinated animals was identified by SNT and ELISA. It was found that there is no difference in the immune response among different vaccinated groups and there is no drawbacks noticed from combination of the two vaccines in the field.

...

Abou zead, A.A., Ali, A.A.H.*and Abd El Hkeem M.M.

...p;Serosurvey of 80 serum samples revealed 5 (6.25%) positive for the presence of IBDV precipitating antibodies. 3 out 12 pooled bursae (25%) were positive to the presence of IBDV precipitinogen. Trials for virus isolation on the CAM of chicken embryos revealed the isolation of 3 IBD viruses from diseased quails. Isolated IBDV viruses '.vere examined by electron microscopy. The disease was reproduced in quails to record the c...

Sahar A. Youssef I and A. A. Shalaby 

... amplified from infected samples, .i.e., 677, 172, 300, 270 and bp for ACLSV, PDV, PNRSV, PPV and ToRSV respectively, Results showed that multiplex PCR products of the expected sizes were obtained for all virus isolates assayed they were tested either alone or in c.ombination in an artificial mixture with no interference. This method saves time and reagent costs compared with rnonospecific...

S.A. Sidaros, S.A. El-Kewey , Hala A. Amin;Eman A.H. Khatab ,  A.A. Emeran l , Samaa Abd El-Khalilk and M.A.S. El-Kady

...rom Tropica Soda Apple Mosaic Virus-CP (TSAMV-CP) Florida isolate (USA) of  Tobomovirus

...
El-Dougdoug, Kh.A. t , S.A. Ghaza12 , A.A. Mousa2, H. Fahmyj and A.R. sofy2 
...ed using double antibody sandwich-enzymelinked immunosorbent assay (DAS-ELISA), woody indicator plants, differential hosts, peroxidase isozymes and activity, total RNA content and reserves transcriptionpolymerase chain reaction (RT-PCR). The severe isolate (ARC) gave the highest OD. value (2.204) in ELISA...

Sabry Y. M. Mahmoud; Maher H. Hosseny and Mamdouh H. Abdel-Ghaffar

...e linked immunosorbent assay (DAC-ELISA) using antisera against PVY, Potato virus X (PVX) and Potato leaf roll virus (PLRV). The results indicated the occurrence of single and mixed infections of three viruses in potato plants. Survey results indicated highly distribution of PVY infected plants, which its yield was affected strongly. Tubers of potato plants which gave a positive reaction t...

Sahar, A. Youssef t ; M.M.A. Al-Dhaher 2 and A.A. Shalaby 

...ncluding yellowing and mosaic pattern or bright yellow bands along major veins. Fan-shaped leaves may or may not be present with mosaic or vein banding symptoms which identified as Grapevine fanleaf virus (GFLV). Detection of the viruses was carried out by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELI

*Amal Abou El-Ela A., M. A. Amer and Eman A. H. Khatab,

...and / or Gompherena globosa. Morphological studies of CarVMV were conducted by light and electron microscopy. Light and electron microscopy revealed amorphous cytoplasmic inclusions in infected leaf cells. In some cases, however, inclusions have a characteristic shape, spindle, circular or sledge-like. Pinwheel inclusions characteristic of Potyvirus which include CarVMV were also observed. The nuclei were variable in size and shape, sometimes complete destruct...

Amina A. II. and M.K. Amin

...ting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of plaques (turbid and clear) have been observed. The average number of turbid plaques was 2.52 x 109 pfu/ml whereas of clear plaques was 9.96 x 107 pfu/n) l. The phage was able to lysis 13 out of 20 strains of Pseudomonas aerugino<...

* Amal Abou El-Ela, A.

...toms. To identify the causal virus, the plants were tested by enzyme-linked immunosorbent assay using antibodies against different Ilarviruses i.e., Apple mosaic virus (ApMV), Prunus necrotic ring virus (PNRSV), Rose mosaic virus (RNIV) and Tobacco streak virus (TSV) Preliminary results revealed the presence of PNRSV in tested rose shrubs. The isolated v...

Manal A. El-Shazly1, A. s. 2 Abdel Wahab and Salwa N. Zein3

...re isolated from Vinca rosae (L). G. Don and onion (Allium cepa L.), respectively. The observed symptoms included systemic mosaic and yellowing for TSWV and necrotic eyelike spots and yellow spots on leaves, and flower stem for IYSV. Both TSWV and IYSV were mechanically and seed transmitted. TSWV was transmitted by two different thrips species, Thrips tabaci L (33.3%) and Frankliniella occidentallis Pergamde (60.9%) whereas ...

S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy* 

...int of BBSV in faba bean sap was I :320 using indirect ELISA, whereas it was 1:280 for CABMV. Both of BBSV and CABMV were detected in stems and all flower parts (sepals, petals, pistils and anthers) in intact seeds and all seed parts (seed coats, cotyledons and embryos) of immature seeds obtained from infected faba been and cowpea plants. Both of the viruses were not detected in seed coat ...

Mohga M. El-Tahlawey, A. Mandour and AE Aboulata

...otic virus (FBNYV) and mosaic causing viruses were inspected during Oct. 2003 to Apr. 2004 on Faba bean fields grown in Qalyubia governorate. Population density of seven different aphid species on faba bean was arranged descendingly as follow: Aphis craccivora (270.9). Myzus persicae. (89.0). A fabae (52.5). Acyrthosiphon pisum (24.3), A. gossypii (4.7). A sesbaniea (1.8). and A. nerii (0.7). Population fluctuation of previous aphid species was also recorded o...

S.Y.M. Mahmoud1 and M. Hashem2

... out of 50 root and soil samples, respectively. The virus was found with percentage of 65% in root samples collected from Kafr EI-Sheikh. The transmission experiments indicated that BNYVV was mechanically transmitted to Chenopodium amaranticolor, C. quinoa, Beta Vulgaris cvs. Pleno. Tripl and Gloria. D. macrocarpa and B. maritema inducing chlorotic local lesions spreads into the veins. The virus was also transmitted by a soi...

A. E. Aboul-Ata, Mohga, M. El-Tahlawey, M. A. Amer and A.M. Mandour

A. A. Kheder1; I. A. M. Ibrahim2; H. M. Mazyadl

...ustify;">Peach rosette mosaic virus (PRMV) was detected from naturally infected commercial peach trees growing under field conditions at El Dakahlia Governorate, using DAS-ELISA. The detected virus is recorded for the first time in Egypt. Virus identification was performed using symptom expression. host range. graft transmission. serological tests i.e., DAS-ELI

A.A.Farrag;  I.A.M. Ibrahim and, H.M. Mazyad

... and Dot Immunobinding Assay (DIBA). Survey was carried out during 2001 to 2003 in different locations on commercial peach orchards. Percentages of infection were 6.3, 6.7, 12.3 and 10.9 in Menofia (Khatatba) . EL-Behira (EL-Nobaria North Sinai (Rafah) and Dakahlia (Metghamr), respectively. ACLSV was transmitted mechanically from peach trees those showing less vigorous and some abnormalities on growth without leaf symptoms to different herbaceous hosts includi...

A-New-Whitefly-Transmitted-Geminivirus

...l analysis, the viral causal agent vector of the two types of symptoms was found to be Bemisia tabaci (Gennadius). Based on diagnostic host species and back inoculation assay two different types of symptoms were found. The first consists of identical Tomato yellow leaf curl virus (TYLCV) symptoms such as severe leaf chlorosis and distortion on tomato plants. named TYLCV-Giza isolate, and the other one showed yellow mo

M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan2 and L.A. Salem2

...gainst Zucchini yellow mosaic Potyvirus (ZYMV). These chemicals were. Potassium sulfate, Magnesium sulfate, Ammonium sulfate, Chelated iron, Chelated zinc, Chelated manganese, and salicylic acid. SAR was determined via disease severity. virus concentration and some biochemical changes i.e., the high level of endogenous sa<...

E.F. Mohamed1 and I.M. Sabra2

...lign: justify;">Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mode of transmission, and ELISA detection. ToMV had a longevity in vitro (LIV) of 9...

E.F. Mohamedl and A.A. Owayss2

...as easily transmitted by sap. ELISA was used efficiently to detect BBMV in leaves of infected Faba bean plants. The present study was carried out to determine the effect or propolis on BBMV. In vitro experiment, propolis reduced the occurrence of mosaic symptoms on Faba bean plants to 80, 70, 60, 75 and 90% of infected plants immediately: to 75, 65, 45, ...

M. Osman1, Kh. El-Dougdoug2, E. T. Abd El-Salam3, R.M. Taha4 and R.M. El-Hamidl

...An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeared in the newly formed leaves in the form of vein banding, severe mosaic, yellowing, malformation...

B. A. Othman; Kh. A. El-Dougdoug; M. H. Abdel Ghaffar and T. F. El-Arabi

... tested rhizosphere soil samples collected from Qalyubia and North Sinai Governorates based on plaque morphology, The extinction spectra of purified preparations showed A260/A280 of 1.25. 1.16. 1.28 and 1.24 for RM1, RM2, RM3, and RM4, respectively. When the purified preparations of RM1, RM2, and RM3 phages examined in transmission electron microscope they showed particles have isometric heads or about 52.5, 58.8 and 64.2 nm in diameter with long contractile t...

M. E. Shawky and A. M. Daoud

...e linked immunosorbent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test (SNT) and 3CD ELISA Kit. Sensitivities of the ELISA

Seham A. El-Zeedy1, Dalia A. Abd El-Moaty1, H. A. Hussein2 and M. A. Shalaby2

...tbreaks of Infectious bursal disease in commercial chicken flocks worldwide are due to the spread of very virulent IBDV. Two Egyptian isolates Of IBDV isolated from Giza (Giza/2000) and Qalyubia (Kal/2001) governorates were characterized by RT/PCR-RFLP assay of a 643 bp hyper variable region Of VP2 gene using BstNI, MboI, and SspI restriction enzymes. The presence of Sspl site in Giza/2000 identified it as vvIBDV while the a...

A. A. El-Kholy1, S. Vilcek2 and A. M. Daoud1

...accines and diagnostic assays for effective control policies.

...

Nahed A. Mohamed l, H. A. Hussein2, Fathia M. Mohamed1 and M. A. Shalaby2

...e hundred and thirty-two samples including buffy coat and organs were collected from apparently health Screening buffaloes in Cairo, Mansoura and Suez governorates in Egypt during the year 1999. Screening of samples by GAPT using standard known anti-BVDV antisera revealed that three positive samples for the presence of BVDV antigen. In a trial for the isolation of the BVDV, 69 buffy coat <...

H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1

... subjected to further passages on MDBK cells infected with non-cytopathic (NCP) strain of BVDV which was homologues (pair) to their genotype (type I). After four passages on cell culture. The local isolates demonstrated enhancement in their CPE and virus replication as determined by virus titration assay. Propagation of these viruses in BVDV-free cells for five pas...

H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3

...tbreaks of infectious bursal disease (IBD) among vaccinated chicken flocks during the period 2002-2003 revealed that all IBDV outbreaks occurred at 18-33 days of age. 70% (21/30) of these outbreaks  characterized by typical clinical signs and gross lesions. The mortality rates in these flocks ranged between 9.4-12.8 % (average 11.1%); 16.4-30 % (average 23.2 %) and 12-23.5 % (average 17.75 %) in commercial broilers. commercial layer replacement pullets an...

M.R. Abd-El Wahab l, H.A. Hussein2, T.M. Asfourl and M.A. Shalaby2

...d fluorescent antibody assay. Histopathology examination of different organs revealed changes similar to those reported with BVDV infection. The existence of BVDV in sera collected from camel population. in the area (Al-Ain city. Western area or Abu Dhabi- Emirate, UAE) where camels were found was confirmed. The antigen was confirmed using antigen capture ELISA and FA as

Ahmed Abd El-Samie H. Ali

...ups were infected intranasally with a virulent NCP BVDV type I (NY-I) and 2 (890) biotypes and kinetic evaluation of its effect of in vivo infection on clinical and immunological parameters. All virus-inoculated calves developed a fever on days 3-7-post infection. BVDV antigens were detected in the buffy coat cells on days 3, 5, and 7 post infections. Most calves developed low neutralizing antibody titers as type I (3, 12, 18 and 44) and type-2 (6, 16, 20 and ...

S. A. Khalil1; M. El-Sayed2; M. M. El-Fayomy2; H. Y. Hassan3 and A. Zaghawa3

...abdovirus which causes disabling febrile infection among cattle and water buffalo. The disease is characterized by inflammation of mesodermal tissue as achieved by muscular shivering, stiffness, lameness, and enlargement of the peripheral lymph nodes. Recently, the disease caused several outbreaks during 2000, 2001 and lastly 2004. The persistence of immunity after natural infection is varied from 6 months to several years. Assessment or maternal antibodies in...

Musaad A. Al-Dubaib

...the present study, serum samples obtained from Holstein cattle, native cattle, sheep, goats, and camels in Al-Qassim, and surrounding regions were tested for bovine leukemia virus (BLV) antibodies for different animal species. Agar gel immunodiffusion was carried out using commercial diagnostic reagents. All samples of herds having positive cases with immunodiffusion were tested by ELI

M.S. Wassel1; Elham A. El-Ebiary1; Soliman, Y.A.l and El-Sayed, M.M.2

...ine besides studying its safety, potency, and the duration of immunity in pregnant and non-pregnant sheep and cattle as well as remaining its antigenic and immunogenic power in animals using its protein analysis and Western blotting.

...

Arwa H. El -Naggar, Nirmeen G. Shafiek, and M. S. Wassel.

...d indirect fluorescent assay (IFA) tests were used to evaluate the hyper immune serum Before conjugation with fluorescent. The antibody titer reach 32 by SNT and 5984 by ELISA and identified by IFA to be Bovine rota. Separation of anti B. Rota immunoglobulins IgG "were done using activated Sepharose 4B followed by conjugation with fluorescein isothiocyanate at PH 9.6. The conjugated I...

N. A. Sherif, Lamiaa M. Omar, S. M. Shafei and Elham A. El-Ebiary.

...of immunized hens by 33% saturated ammonium sulphate solution. The anti-rabbit immunoglobulin-Y (IgY) was purified by using affinity chromatography. The obtained globulins were divided into 2 parts. The 1st one was conjugated with peroxidase and the 2nd part was conjugated with fluorescein isothiocyanate. Both conjugates gave a strong positive result when diluted 1:2500 and 1:50. respectively. The results indicated that they could be used successfully for dete...

Ausama A. Yousif1,3, Sandy M. Watkins1, Lyle J. Braunl, David J. Hurley2 and Christopher C. L. Chase1

 

...values obtained from the samne reaction tube in a real-time RT-PCR. This was done in bovine and ovine cultured cells following simultaneous- or superinfection with different biotypes and genotypes of BVDV. The noncytopathic BVDV-2 890 blocked superinfection with the cytopathic BVDV-I strain Singer in both BT and MDOK cells. The cytopathic BVDV strains greatly reduced the relative concentration of superinfecting but not coinfecting cytopathic and noncytopathic ...

Maria Kikelomo Adegun1*, Femi Godwin Ekundayo1, David Daisi Ajayi2 

...esigned that included cassava peels ensiled with urea (UTCP) and graded amounts of Gliricidia sepium fodder (GSF). Sixteen yearling WAD rams with an average weight of 12±1 kg was randomly assigned into four treatments (T1, T2, T3, and T4) in a ten-week trial. The rams assigned to T2, T3, and T4 were fed 90% UTCP+10% GSF, 80% UTCP+20% GSF, and 70% UTCP+30% GSF, in that order while T1 served as the control group. The data did not show a statistically sign...

Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2

...fty jugular venous blood samples (about 5ml) were collected. The total infection rate of Leucocytozoon spp. in the blood smears was 10% (5/50), which divided into males 6% and in females 4%, while by qRT-PCR was 4% (2/50) with an equal ratio between males and females (2%). In conclusion, it is the first diagnosed study in Baghdad city to detect Leucocytozoon spp. in broiler chickens by using qRT– PCR. 
 
 

Sulake Fadhil Al-Zubaidi1*, Ghusoon A.A. Alneamah2, Ali Saleh Mahdi1, Abdulraheem Abduljalil Wali3

...ntinue until discharge disappeared. Aglepristone was repeated after 24 hours and seven days if needed for groups I and II. Group III: six bitches with open pyometra received Misoprostol (Cytotec®) given intravaginally as 200 mcg/bitch. All groups received a course of antibiotics. Group IV: two bitches treated by ovariohysterectomy under general anesthesia. Bacterial isolation was made on chromogenic agar to identification of bacteria associated with pyomet...

Nahariah Nahariah1*, Hikmah Hikmah1, Faridah N. Yuliati1, Endah Murpiningrum1, Abdul W. Jamaluddin2, Titis S. Kusuma3, Andi Febyana4, Kartina Kartina1

Makmun1, Imam Mujahidin Fahmid2, Muhammad Saleh S. Ali2*, Muhammad Yamin Saud2, Rahmadanih3

...nformants uses purposive sampling of farmers and the Animal Husbandry Department of Blitar Regency. The study’s findings indicated that actors involved in the laying hen farming business were small farmers, medium farmers, large farmers, farmer groups, poultry shops, the Animal Husbandry Department, Ministry of Agriculture-Directorate General of Livestock and Animal Health, feed/medicine companies, egg collectors/middlemen, local traders, inter-regional/...

Luthfiralda Sjahfirdi1*, Lisa Nurfalah1, Deanvi Fahira Wisnuputri1, Entang Iskandar2

...e-linked immunosorbent assay (ELISA). Our results showed that the average percentage of behavior observations (K2 and K5), namely was behavior moving 61%, unseen 15%, resting 8%, social behavior 8%, autogrooming 5%, feeding 4%, and reproductive behavior (1%). The presence of reproductive behaviors (1%), such as sniffing and licking the genitalia of a mate, indicates pair harmony that could...

Nguyen Binh Truong1,2*, Ho Xuan Nghiep1,2, Tran Trung Tuan1,2

...), Brocken rice (Br), Cassava chip (Ca) and Wheat (Wh). The proportion of combination (% dry matter intake) in two energy feed sources was 15% and 15% such as MaCa, MaWh, BrCa and BrWh. The results show that dry matter intake per body weight of BrWh (3.42%) and BrCa (3.40%) treatments tended to high compared to the MaCa (3.29%) and MaWh (3.08%) treatments. The organic matter digestibility (%) of BrCa was not different (P>0.05) with MaWh and BrWh but it was ...

Nurhashunatil Mar’ah1, Safika Safika2*, Agustin Indrawati2

...). The cattle udder swab samples showed resistance to ampicillin and amoxicillin (100%), cefotaxime (75%), and ceftazidime (50%). Gene resistance detection found that water samples encoded blaTEM gene (100%), Udder rinses water encoded blaTEM (80%), blaSHV (20%), blaCTX-M (20%) and udder swab samples detected the presence of blaTEM genes (40%). K. pneumoniae virulence factor genes: mrkD de...

Rawaa Saladdin Jumaa

...acted from the collected samples was amplified using conventional PCR by amplification of the P4b gene. The positive samples had been inoculated on chorioallantoic membrane (CAM). Thickening of CAM were seen in the first passage, whereas pock lesion at the second (2nd) and third (3rd) passages. The chicken embryo fibroblast (CEF) cells propagated by the ...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

... of the antigen, immunoassay analysis and blot-hybridization techniques were employed. Obtained results showed that 1mM isopropyl ß-D-1-thiogalactopyranoside induction at 2℃ for 18 hours was the most optimal condition for expression of recombinant GM6 T. evansi antigen. From a 500 ml of growth medium, a total of 3.4 mg of recombinant protein was successfully obtained. A recombinant GM6 antigen of T. evansi encoding a 30 kDa polypeptide was developed, w...

Tarek Refaay, E.A. Elshafiee, Hayam A. Mansour and Maha A. Sabry*

 

...t. For this purpose, 648 samples including raw chicken meat, cooked chicken meat, raw beef meat, cooked beef meat, food handlers and equipment swabs were collected from 54 food outlets in some touristic cities in Egypt. E. coli O157 was 1.1% (7/648) and 1.2% (5/432) in all examined samples and food samples respectively. Cooked chicken samples were the mo...

Faisal Alzahani1* and Mohammed Abu El-Magd2*

...id. To get rid of this disadvantage and increase the anticancer effect of CAPE, CAPE-loaded chitosan nanoparticles (CNPs) were used. The anti-tumor effects of CAPE and chitosan CNPs on cancer cells have been separately studied but the precise epigenetic molecular mechanisms for the combined therapy are still unclear. This study aimed to investigate the epigenetic mechanism of CAPE and/or C...

Ahmed Kadhim Munahi1* , Hameed A. Al-Timmemi2 

...y is aimed to apply chitosan nanoparticles on spinal cord regeneration in dogs. For this objective, sixteen healthy mongrel dogs were used. They were divided into two equal groups (n=8) at random. All dogs underwent dorsal laminectomy and left lateral hemisection at the level of the second lumbar vertebra. In the control group, the hemisections were treated with 0.2 mL of phosphate buffer sa

Saddam Hummadi1, Nadia Al-Falahi2* 

...m small intestine submucosa (SIS) and AgNPs composite for accelerate the healing of infected wounds and improve cosmetic outcomes. A 5% w/v SIS hydrogel was prepared and formulated with 100 µg/ml AgNPs to evaluate its effect on healing of infected wounds in rabbit. Forty eight adult rabbits aged 8-12 months, weighing 1.5-2.5 kg, were divided randomly into three equal groups (n=16) after inducing infected wounds. In control group (GI), the infected wound...

Estabraq Hayder Khayoon, Ammar Ahmed Abdulwahid*  

...of the experiment, blood samples were taken using the heart puncture technique after the subjects had been given an intramuscular injection of ketamine and xylazine to induce anesthesia. The findings revealed the exposure of Acrylamide to rats significantly reduced their tendency to gain weight, a marked rise in the concentration of malondialdehyde and decrease levels of Total antioxidant in blood serum and behavioral differences like Acrylamide greatly de...
Saliha Bashir*, Muhammad Shafique, Muhammad Shahzad, Muhammad Sohail Anjum and Ahmad Ali Shahid
...and pigment spots in KPK sample set. Based on unique iris patterns, these phenotypic traits would be helpful for individuals’ discrimination in the population. In future, there is need to explore genetic associations and functional differences of these traits.

...
Raed M. Al-Atiyat1,2*, Gamaleldin M. Suliman3, Khaled Abu-Alruz4, Firas Al-Zyoud5, Amer Mamkagh6, Ahmed M. El-Waziry7, Abdullah N. Al-Owaimer8 and Rifat Ullah Khan9

Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2

...evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP gel, while the group B (n=6) animals were treated with sterile saline. Full-thickness (3 x 3 cm2) skin wound...

Muhammad Wasif Gulzar* and Jawad Hussain

...h to creating AI is necessary, as is supporting openness, continuous quality control, and a risk-benefit analysis. We think AI tools should only be used in clinical settings once they have undergone thorough validation. Once that is done, we think they should be continuously checked in clinical settings. Furthermore, it is imperative that veterinary practitioners receive precise instructions and guidelines for the best possible use of these tools. 

...
Zeynep Karapinar1* and Mehmet Özkan Timurkan2
... 7 (36.84%) of the stool samples collected from 19 cats with diarrhea and vomiting symptoms. Genotyping and phylogenetic analysis of the positive samples were performed. It was observed that five of the FPLV positive samples obtained were in the G3, one sample was in the G1, and one sample was in the CPV-2b group. FPV ...

Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2* 

...to broiler meals. At the same time, the concentrations of alanine transferase, aspartate transferase, and uric acid were significantly (p < 0.05) lowered. Additionally, the triglycerides were dramatically (p < 0.05) decreased while the high-density lipoprotein composed a higher proportion than the low-density-lipoprotein of the total cholesterols (p < 0.05). Additionally, as dietary FS levels increased, plasma corticosterone (CORT) and malondialdehyde...

Sarah G. Yousef1*, Ahmed Shehta2, Hend M. El Damaty1 and Hussein A. Elsheikh3

...gypt. Seventy-five fecal samples were collected from diarrheic and healthy dairy cows that came into contact with diarrheic ones for molecular screening. Clinically, 32 of 75 examined cattle showed variable degrees of body weight loss and diarrhea. The PCR targeting the insertion sequence IS900 gene revealed that 22.6% of examined cattle were positive for MAP. A rate was significantly higher in diseased older cattle than in young cattle with no symptoms of the...

Sajjad Khan*, Naila Chand, Abdul Hafeez and Nazir Ahmad

...r, gizzard, pancreas, bursa, spleen and thymus was recorded for the groups studied. Significantly higher (P<0.05) VH, lower (P<0.05) CD and higher (P<0.05) VH:CD in duodenum, jejunum and ileum were recorded in synbiotic group D followed by C, E and B. Similarly, supplementation of synbiotic in group D and Bacillus subtilis group C resulted in complete eradication of E. coli, Salmonella and C. perfringens from ileum, caecum and colon of experimental br...
Asad Ullah1, Hayat Ullah2, Muhammad Lateef3, Imrana Niaz Sultan4, Afrasiab Khan Tareen4 and Muhammad Waseem Khan4,5*

Lingang Dai1,2,3, Xiang Chen1,2,3, Jiajin Huang1,2,3, Jiali Xu1,2,3, Meimei Xiao1,2,3, Jiajing Chen1,2,3 and Yong Ruan1,2,3*

...a, however, it has the disadvantages of smaller individuals, slower growth rate and low feed conversion rate, so, we introduced Boer goat as the male parent and crossed with Guizhou white goat to obtain F3 generation of Boer × White goat hybrid to improve its production performance. To study the effect of PGAM2 gene polymorphisms on the performance of F3 generation hybrid goats, the relationship between PGAM2 gene polymorphisms and slaughter traits, meat...

Kouengoua Kouengoua Armelle Prudence1*, Yessinou Roland2, Nankam Chimi Roland1, Fotsac Dzousse Muller1, Kouam Alain1, Djuikwo Felicite1, Facho Balaam1, Awah Ndukum Julius3, Farougou Souaibou2 

... to prevalence, host, causal agents, methods of diagnostics, risk factors. After several screenings, six articles were considered for this systematic review. East and Southern Africa countries carry on most researches on bovine tuberculosis in wildlife. Available studies assessed the status of wild mammal, carnivore. Further researches to improve the knowledge on the status of bovine tuberculosis in wildlife in Africa are still needed in order to allow proper ...

Ahmed M. Manthoor, Ali H. Saliem* 

... the extract against the same bacteria by Fe-Scanning Electron Microscopic(Fe-SEM). The extraction ratio was (15%) and the phytochemical analysis referred to the presence of alkaloids, flavonoids, steroids, carbohydrates, tannins, free amino acid and protein. While the gram staining, cultural characteristics, biochemical tests, Vitek 2 system and PCR tests referred to that bacteria was E. Coli. Bacteria was susceptible to the alcoholic extract of P. oleracea a...

Hillary M.O. Otieno1,2*

...ing herbicides is a time-saving technology that could increase crop productivity, revenue, and food security among resource-constrained farmers in the Sub-Saharan Africa region. However, the land sizes are small and pose a big question as to whether it is economical to embrace the technology. Again, if not used well, these products could be harmful to humans and other non-targeted organisms that significantly contribute to the well-being of the ecosystem. This...

Shakir Ullah1* and Lubna Shakir

...it was present in Ghargu samples at even < 2 months. The phasing out by red fluorescing compounds in stems (SG5) and leaves (SG7) as the plant age increased was attributed to the loss of chlorophyll (green pigments) as a plant part matures. The results indicated that the relationship between phytochemical yield and age of harvesting is not linear. Harvesting at a lower age of < 2 months gives fewer phytochemicals than at 3-4.5 months, which seems to be o...

Tabinda Nowsheen1, Sayed Wadood Ali Shah2, Ali Hazrat1*, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar4 and Muhammad Ajmal Khan3

... flavonoids, terpenoids, saponins, tannins, phenolics steroids and carbohydrates but deficient of proteins. In minimum inhibitory concentration assay, the crude methanolic extracts showed significant inhibition against all tested bacterial strains at25, 50 and 100 µg/ml. The methanolic crude extract of A. maritime various parts showed MIC of 37.5µg/ml for S. aureus which is gram-positive bacteria followed by 75&m...
Geeta Moolchandani1, Jaiperkash Moolchandani2*, Nasima Iqbal3
Syed Mohsin Turab4, Muhammad Kashif5 and Lubna Farooq6
Rehan Ahmed Siddiqui1,2*, Shabana Usman Simjee2,3, Nurul Kabir4, Muhammad Ateeq3,5, Kevin Joseph Jerome Borges6, Muhammad Raza Shah3 and Rahman M. Hafizur2
...kidney due to various causative agents. Renal insult can result from ischemia, drugs, renal obstruction, inflammation, or extensive muscle injury. The current study focused only on Rhabdomyolysis-induced AKI. We make an attempt to uncover the preventive efficacy of cinnamic acid (CA) and CA-conjugated gold nanoparticles (CA-AuNPs) against rhabdomyolysis-induced AKI induced in mice. CA (50 mg/Kg) and CA-AuNPs (30 mg/Kg) were given to the animals for four days. ...

Taleeha Roheen1, Shagufta Kamal1*, Shazia Anwer Bukhari1, Ghulam Mustafa1 and Saima Rehman2

...teria in rice paddy soil samples. The evolutionary analysis indicated a phylogram with five monophyletic groups (MPG-I to MPG-V). Results revealed that three isolated species (Sphingomonas sp. MG-1, Sphingomonas sp. MG-2 and Sphingomonas sp. MT-2) were in a close evolutionary relationship while the fourth isolated species (Sphingomonas sp. MT-1) appeared in MPG-II and showed strong evolutionary relationships with Sphingopyxis terrae and Novosphingobium capsula...

Skeikh Mustafizur Rahman1*, Mst. Shirin Sharmin Khan1, Yousef Ahmed Alkhamis2,3, Roshmon Thomas Mathew2, Md. Moshiur Rahman1, Mohammed Monirul Islam4, Md. Asadujjaman5 and Md. Golam Sarower1

...rotein and other indispensable nutrients. This study aimed to investigate the proximate components (e.g. protein, lipid, moisture and ash) of eleven non-commercial marine fish species namely Johnius argentatus, Harpodon nehereus, Cynoglossus lingua, Johnius elongatus, Sillaginopsis panijus, Pomadasys hasta, Setipinna phasa, Megalaspis cordyla, Rita rita, Gonialosa manmina and Scatophagus a...
Nael Abutaha, Fahd A. AL-Mekhlafi*, Khalid Elfaki Ibrahim, 
Mohammed S. Al-Khalifa and Mohamed A. Wadaan
...UV-EC). The larvicidal assay was performed on the third instar stage as recommended by WHO. The phytochemical analysis showed the presence of alkaloids, sterols, and phenolic compounds. The GC-MS analysis revealed the presence of 5 compounds, of which α-Copaene constituted 61.95% of the extract. The hexane extract showed good larvicidal activity, with an LC50 = 33.9 μg mL−1 and LC90 = 64.1 µg/mL at 24 h post exposure. The mid...

Seyed Ahmed Reza Hashemi1, Teymour Aminrad1, Asadullah Ali Muhammad2*, Mastooreh Doustdar3 and Parastoo Mohebi Derakhsh3

...thern Oman Sea (Iran) by sampling sea cucumbers at nine sites from March 2018 to March 2019. The sites included Aliabady, Ramin, Beries, Gurdium, Kachoo, Beries plane, Tis, Pasabandar and Pozm. 125 female and 121 male specimens were measured for total length and total weight, with mean ± standard deviation of 28 ± 4 (21 - 33) cm for males and 25 ± 6 (14 - 39) cm for females, and 421 ± 128 (191 - 7...

Sena’a M. Hussein1,2, Raffal A. Omar3* 

...single and repeated lumbosacral injection of liposome carrying lidocaine in comparison with lidocaine hydrochloride. A total of 24 male local breed dogs were divided randomly into the lidocaine group that received 4.5 mg/Kg of 2% lidocaine and liposome group which received the same dose of lidocaine carried by liposome. Pinprick and spinal reflex tests were evaluated each minute for the first five minutes following lumbo

Wisnu Jaka Dewa1,2, Ekowati Handharyani3*, Sri Purwaningsih4, Silmi Mariya5 

...gene expression, and the safety of using red eye sea snail extract as an anti-cancer. 

...

Haseeb Zafar1, Asghar Ali Kamboh1*, Zain-Ul- Abideen1, Muhammad Kamran Taj2, Sibtain Ahmad3 , Umbreen Zafar2 

...l agents. A total of 150 samples from a variety of sources (cecum, feed, bedding, water, air, and flies; n = 25 each) were collected from five distinct broiler farms (n=30/farm) in the district of Quetta. Samples were streaked on selective (Xylose Lysine Deoxycholate agar) and differential medium (MacConkey’s agar) plates and incubated at 37°C for 24 hours. After isolation, organisms were further identified through Gram staining, and different bioche...

Abdul Hassan1*, Shahnaz Akhtar1 and Muhammad Ishaq2

...ea, that indicates the disadvantageous nature in livelihood asset of agricultural households, uncovering the subtle living standard of agricultural households and lacking prospects of economic development. The research findings reveal that human capital, financial and social capital of livelihood asset has positive significant effect on the choice of agricultural households’ livelihood strategies in the models of rural households type II and rural househ...

Alveena Izhar1*, Basit Ullah2, Rabia Asghar1, Aqeel Ahmad1 and Hassam Bin Mujahid1

...tion of radish (Raphanus sativus L.) cv. ‘Green Neck’ was conducted at the Horticulture Research Farm, The University of Agriculture, Peshawar; Pakistan during the session (2020-2021). The experiment was designed in Randomized Complete Block in split plot arrangement with three times replication. The main plot consists of phosphorus levels i.e. (0, 25, 50, 75, 100 kg ha-1), whereas steckling sizes i.e. (10, 20, 30 cm) were assigned to subplots. The...

Ram Prasad Ghimire

...ayero (Woodfordia fruticosa Kurz), Musure katus (Castanopsis tribuloides (Sm.) Lindl.)), Bamboo (Bamboosa arundinacea (Retz.) Willd.), Titepati (Artemisia vulgaris Willd.) and Sal ((Shorea robusta Roth.) and a mixed fodder were used as the treatments. The results of the study revealed that W. fruticosa was obtained best for nutrient composition (6.94±2.32% total ash and 10.625&plusm...

Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*

...ality. The difference in sample collection time after administration is assumed to have a positive effect on testosterone concentration. This research aimed to determine the effect of PGF2α injection and the optimal time for sample collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into two treatments. In treatment 1 (P1), the bull received ...

Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin

...nization for work, serum samples were taken for passive hemagglutination test (PHA); Immunoglobulin G(IgG) detection using a chemical immunosorbent assay test. the outcomes of PHA test revealed that Folcord inhibited humoral immune response, with a considerable rise in antibody titer in the third and second groups, but decreased serum antibody levels in the first group. Furthermore, the (IgG) titer in serum is measured using...

Nasir Shah1,4, Muhammad Ibrahim1*, Zarnosh Habib2, Kalsoom3 and Zahir Shah4

...ria multiflora, Achillea santolina) and their various concentration (2, 1, and 0.5%) were used for the purpose. Firstly, the artificial diet of fruit flies was subjected to these treatments, while on the other hand chikoo fruits which were used for flies to settle on, were dipped in same concentrations of these plant extracts, and dried under shade and exposed to peach fruit flies for feeding for 15 days. All the three plant...

Faramin Javandel Soum Sarai1, Mir Daryoush Shakouri2* and Alireza Seidavi3*

...d on corn-soybean meal basal diets with no additive (control) or added chromium picolinate (400 mg/kg), chromium picolinate (400 mg/kg)­­+­ vitamin C (240 mg/kg) or chromium picolinate (400 mg/kg) +formic acid (0.5%) form 29 to 42 days of age. All supplements significantly improved daily feed intake, daily weight gain and feed conversion ratio of birds compared with the control (P<0.05). The treatments reduced the abdominal fat (P<0.05) and h...
Rahmat Ullah Khan*1, Karim Gabol1, Abbas Khan1, Asif Sadam2, Waheed Ali Panhwar3, Hamid Ullah5, Muhsin Ali4, Gul Bacha Khan4 and Habib Ul Hassan1
... bulbul (40) and greater sand plover (40). In the Gharo Creek area significantly (P<0.05) higher diversity was recorded as compared to the Scrubland forest area and Bhambor (Terrestrial) Fort area. However, threats responsible for the decline of biodiversity and density of avifauna include destruction of habitats, soil erosion, human interaction, drought, illegal killing, predation, water pollution, scarcity of food, and unfavorable environmental conditions...

Siqiang Li1,2, Tiantian Wang1, Peng Sun1, Airong Gao1, Xin Gong1, Yuanhong Xu2, Baogen Wang2, Jun Wu1* and Bo Liu1*

...ol enzyme to modify oligosaccharides and their analogues in structurally homogeneous and defined forms in vitro. This study was aimed to explore the acquisition of an effective MDS I in vitro. For this purpose a Pichia pastoris strain GS115 harboring a recombinant MDS I derived from Trichoderma reesei was constructed via conventional molecular cloning methods, and expressed in a 5-liter fermentation tank. The target protein was purified in three-step purificat...

Limbang Kustiawan Nuswantara, Eko Pangestu, Marry Christiyanto, Santoso Dwi Pratomo, Elyza Zahrotul Muhtaromah

Heni Indrijani*, Asep Anang, Muhammad Farhan Fadilah, Nena Hilmia, Maya Fitriani

...iod was examined using a sample of 319 chickens selected from two different groups. The first group consisted of 13 males and 147 females Sentul Debu chicken aged 11 months, while the second group consisted of 13 males and 146 females Sentul Kulawu chicken aged 14 months. The hatching eggs produced from breeding one line and crossbreeding were hatched for 21 days, resulting in fertility, and hatchability was calculated as a value of the heterosis effect. Each ...

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

...ected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated experiments. The results showed that sperm motility, viability, and plasma membr...

Henny Leondro1*, Peni Wahyu Prihandini2, Yuli Arif Tribudi3, Aju Tjatur Nugroho Krisnaningsih1, Dimas Pratidina Puriastuti Hadiani1, Agung Kusuma Wijaya4, Dyah Tuwi Ramsiati2, Mochammad Chanafi2, Wahyuni Indah Wulansari2

...acteatum, Vigna and Stylosanthes guainensis. The proximate test was used to obtain chemical component values from herbaceous legumes. The results showed that the highest and lowest potential biomass production is C. ternatea and Vigna, respectively. Furthermore, M. bracteatum had a higher DM percentage (29.12%) value than other herbaceous legumes. C. ternatea had the highest CP (21.96 %DM) and TDN (59.81 %DM). In conclusion, the legumes studied have high nutri...

Nguyen Binh Truong1,3*, Nguyen Ba Trung1,3, Nguyen Thi Bich Hạnh2,3

...rage weight gain was the same as digestive CP in the present study. It was 552 g for NDF43 treatment and 517 g for NDF55 treatment. Therefore, the levels of NDF well for Charolais crossbred cattle in this study from high to low were 43, 47, 51 and 55%. The likely benefit of 51% NDF will be an increase in the utilization of fibrous roughage and daily weight gain from 9 to 12 months of age in the Charolais crossbred cattle diet.
 
Ke...

Kadhim Kh. K. Al-Khayat1*, Athmar K. A. Al-Azawi2

...al cavity of 14 positive samples as balloon filled with clear fluid, white floating scolex (evagenated or invagenated) or pus-filled in the older cysts , distribution of cysts was 47.27% (26/55) in mesentery, 21.82% (12/55) in liver, 21.82% (12/55) free within abdominal cavity and 9.09% (5/55) in omentum with Significant differences (P<0.05).  The cyst diameters range between 7-21mm. in length and 4-10mm. in width. The PCR amplification results of 14 p...
Pratap A. Divekar1,2*, Sampat K Patel2, Guru Pirasanna Pandi G3, Manimurugan C2,4, Vikas Singh2 and Jagdish Singh1
...ct pest population. Spinosad, emamectin benzoate, indoxacarb and chlorantraniliprole were also the found best treatments in controlling DBM and CB. No phytotoxic symptoms were observed in any treatment after spray application. Chlorpyriphos, deltamethrin, lambda-cyhalothrin and flubendiamide were found adverse to natural enemies. Thus, spinetoram, spinosad, emamectin benzoate, indoxacarb and chlorantraniliprole are recommend...

Muhammad Yasin1, Azhar Abbas Khan2*, Sana Rubab2 and Sumaira Maqsood3

...f Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) in combination with commercial diatomaceous earth (DE) formulation Inert-PMS alone and in integrate manners against these pests. Beauveria bassiana was applied at 1×106 and 1×107 conidia/ml alone, or in combination with DE at 50 and100 ppm. Adult insects were exposed to the treated wheat for 5, 10 and 15 days at 27±2 ºC and 65±5% r.h. in an incubator. The mortalit...

Shakir Ullah1*, Lubna Shakir2, Ghani Subhan3 and Mohammad Sohail4

...ely as compared with the same variety at full irrigation. Varieties had significant differences in plant weight, number of pods per plant, number of seeds per plant, harvest index, and seed yield. In this study, the soybean cultivars exhibited high sensitivity to water stress. Based on the study, the 448 cultivar is recommended due to its higher seed yield and yield components under the given conditions.

...

Joseph Oyiguh Abraham1, Cornelius Arome Omatola1*, Martin-Luther Oseni Okolo1, Ruth Foluke Aminu1, Emmanuel Edegbo1, Olubunmi Marvelous Emurotu1, Danjuma Muhammed2, Jesse Joseph Chock3, Helen Ojomachenwu Ocean4 and Yahaya Ocholi5

...using commercial immunoassay test kits. Relevant demographic information was obtained from each participant with a structured questionnaire. Of the 1000 participants, HBV, HCV, and HIV were detected in 28 (2.8%), 2 (0.2%) and 3 (0.3%), respectively. However, no participant was simultaneously infected with any two or three viruses. The age group 21-40 years demonstrated greater exposure to HBV (7.57%), HCV (0.37%), and HIV (1.17%) infection than the others. Mor...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
...ast genome from 30 fecal samples of Taohongling sika deer were amplified with universal primers and sequenced by high-throughput. We found that 28 out of 30 valid samples had a total of 677,856 valid amplified sequences with an average valid sequence of 24,209.14±323.83. A total of 326 OTUs were obtained from 28 valid samples, and the MP, SS, and ...

Pankaj Gargotra1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3

...ree different doses in basal feed containing 23% C.P and fed to brooders for a period of 90 days. Fecundity, GSI, gonad histology, hormone (cortisol and progesterone) variations, breeding performance and growth parameters were recorded. Significant differences in the growth and breeding performance of female brooder under different treatments were noted. Among the selected vitamins, incorporation of vitamin C at 150 mg /kg of diet resulted in maximum mean weig...

Amina1, Muhammad Zahid Rashid1*, Muhammad Asim Rashid2 and Amina Rashid2

...l Research Institute, Faisalabad, Pakistan during the year of 2019-2021. Results revealed that Sogoya germplasm have maximum survival (85.71%), number of leaves (16.55), leaf area (62.41 cm2), No. of flowers (38), No. of fruits (46), fruit length (41.69 mm), fruit weight (12.12) and yield (634.2g/plant) was included. While in qualitative characteristics germplasm Sogoya revealed highest total soluble solids (10.85%), total sugars (8.61%) and vitamin C (66.24 m...

Riaz Alam1*, Muhammad Sajid2, Imtiaz Hussain3, Gulzar Ullah1, Hussain Shah3, Muhammad Arshad Farooq3 and Rashid Muhammad4

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ali Zohaib2 and Ishtiaq Hassan3

...farmers were included in sampling frame with non-probability sampling procedure for field survey as well as six field experiments were conducted in Gujranwala zone, Pakistan to evaluate four sowing techniques, i.e., CT, happy seeder, ridge sowing and SS with Randomized Complete Block Design. As compared to CT the SS has been estimated as resource conservation technique regarding sowing time (62.50%), irrigation time (7.69%),...

Muhammad Nadeem1, Muhammad Naveed2,3*, Muhammad Shafiq2, Irfan Rasool2 and Muhammad Afzal Zahid2

...ly, resistance against fusarium wilt and ascochyta blight compared to the existing varieties. The evolution of this strain commenced in 2002-03 cropping season by crossing K-90399 as a female parent with K-52582 as a male parent. The female parent had high yield potential, whereas the male parent had wilt resistance and was developed through introgression breeding using ILWC-126, an accession of Cicer reticulatum. Henceforth, the genetic variability was create...

Anisa Muthia Fakhira1, Madihah Madihah1, Susi Susanah2, Erick Khristian3, Ratu Safitri1*

... to ascertain the iron dosage that affects changes in pancreatic beta cell structure and fasting blood glucose levels in male Wistar rats (Rattus norvegicus). The study using 7 treatment groups with 4 replications. Iron dextran (ID) was administered intravenously in three 3-day intervals, with cumulative doses of 10, 20, 30, 40, 50, and 60 mg/kg BW, whether the control injected with physiological sodium chloride solution. The results of the study showed that b...

Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Vincentia Trisna Yoelinda1, Sarmin1, Yuda Heru Fibrianto1, Pudji Astuti1

... songbird contest. Fecal samples were collected from twelve birds, distinguishing between amateur and experienced groups, both before and after the songbird contest, and preserved at -20°C. Corticosterone and testosterone hormone levels were assessed using an ELISA kit, and the acquired data were tested for normal distribution and homogeneity, followed by analysis through the T-test me...

Eman Said El-Hadad*, Hesham Ahmed Madian, Mahmoud A.E. Hassan, Mohamed Fahmy Saad, Abdelghany M. El-Shhat, Entesar Zakaria Eliraqy

...roup (G1) was fed on a basal diet, while G2 and G3 were fed the basal diet with 0.2 and 0.4 g AR/kg, respectively. At the end of an experimental period of three months, five ganders were slaughtered for blood collection and histological study. Hematological and plasma biochemical parameters, liver and kidney function, and antioxidant markers were determined. Results showed that RBCs and lymphocyte increased, while neutrophil...

Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab

...ioactivity of the hydrolysate. A laboratory experimental method using a completely randomized design (CRD) was used in this research. In this study, chicken head protein was hydrolyzed using different combinations of bromelain (b) and papain (p), namely CHP (without hydrolysis); CHP1 (0.25% b and 0.75% p); CHP2 (0.5% b and 0.5% p); and CHP3 (0.75% b and 0.25% p). The data were analyzed using one-way analysis of variance followed by Duncan’s multiple rang...

Noor A. Namaa*, Hayder A.N. Al-Zamely

...sues utilizing the two assays of Western blotting and Bicinchonic Acid. A total of 60 Wister rats were chosen, prepared, and divided equally into three groups: T1, T2, and negative control, which received only distilled water daily and no other treatment. T1 was given a daily dose of (500 mg/kg) of Ginseng extract, while T2 received a daily dose of 250 mg/kg of Ginseng NPs. After a 60-day experimental period, all study animals were put to sleep using chlorofor...

Elly Roza*, Salam N. Aritonang, Yulia Yellita, Hilda Susanty, Rizqan

...ah buffaloes as research samples with the following feeding: P1 = basal feed (10% of body weight); P2= 30% sweet potato leaves + 30% cassava leaves + 40% of P1 + urea saka block (USB); P3 = 40% sweet potato leaves + 40% cassava leaves + 20% P1 +USB; P4 = 50% sweet potato leaves + 50% cassa

Wael Halawa1, Raed M. Al-Atiyat2*, Jamal Abo Omar1, Faisal S. Rashaydeh3, Fath Ullah4, Shakirullah5 and Berkant Ismail Yildiz6

...ing methods on newborn Assaf lambs growth performance and mortality. A total of 97 lambs were used in the study, with lambing periods starting from October 2020 to January 2021. Ewes’ parities were first, second, and third. After lambing, lambs were randomly distributed according to the suckling method into three groups: natural suckling (G1) (39 lambs), lambs fed completely with milk replacer (G2) (29 lambs), and lambs fed milk replacer at a rate of 4 t...

Hossam F. Abou-Shaara

...showed approximately the same effect on the ability of bees to tolerate low temperature. Some morphological characteristics (head width, forewing length and width, wax mirror longitudinal and wax mirror transversal) used as indicators to body size and the development of glands. These characteristics showed higher values in yeast group than the other groups. In light of this study, yeast feeding is promising and recommended a...

Peixia Yu1*, Lijun Bo2 and Xueyin Song1

...up S), I/R group, normal saline (NS) I/R group, and low, medium and high dose Fen groups, where in high dose group: Fen1:2μg/kg; Fen2:4μg/kg; Fen3:6μg/kg. The measured items included heart rate (HR), mean arterial pressure (MAP), left ventricular developed pressure (LVDP), ±dp/dtmax, malondialdehyde (MDA), superoxide dismutase (SOD) activity, creatine phosphokinase MB (CK-MB) and cardiac troponin-I (cTnI). The total apoptotic cardiomyocytes, B ...

Jiaojiao Wang, Wei Liu, Qingqian Lin and Jianhua Hou*

...ed using a line transect sampling method at the eastern airport of Hebei Province from June to August, 2019. In total, 36 species belonging to 11 orders and 22 families of birds were recorded. Disturbances such as noise from aircraft flights affected the number of species and the individual density and diversity of birds. However, different areas were affected to different extents, and three areas on the same side were the m...

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

...ts further revealed that samples from the foregut and hindgut shared similar banding patterns. Although the midgut was the least diverse segment, there were unique bands that can be further investigated. By using species-specific primers, most fibrolytic bacteria were detected in the foregut and hindgut, whereas most non-fibrolytic bacteria were located in the foregut and midgut. This study provides a preliminary glimpse of the bacterial microbiota profile of ...

Suhad Mohmmed Alrobyrai1 , Falah Muosa Al-Rekabi2*

...each). Half animals were sacrificed after 30 days, the rest after one week of treatment withdrawal. Investigation included body weight, biochemical markers (ALT, GGT, PT, INR), and liver histopathology. After 30 days, G5 showed a significant (P ≤ 0.05) increase in body weight (29.2±1.32) compared to the positive control (20.8±1.2). After withdrawal, G5 displayed a significant (P ≤ 0.05) increase in body weight(31.4±1.17) compared to ...

Qamer J. Jadoaa, Raffal A. Omar*

...dropping isotonic normal saline to prevent thermal necrosis. The experimental animals were divided randomly into three equal groups, each group include fifteen rabbits (n=15). control without any additive. group 1(lidocaine hcl). Which applied daily single dose of 2% lidocaine Hcl 2 mg/Kg. B. W. for five days post-operation (P. O.), while the group 2(diclofenac) applied 3.75% of 20mg/Kg. B. W. histopathological specimens were taken at the end of the 7th,14th, ...

Frennada Lu’fatul Azizah1, Osfar Sjofjan2*, Eko Widodo2

...ulated as follows: T0 (basal feed), T1 (basal feed + 0.5% bacitracin), T2 (basal feed + 0.5% BSF larval oil), T3 (basal feed + 0.5% BSF pre-pupal oil), T4 (basal feed + 0.5% BSF pupal oil). The data were collected and analyzed using analysis of variance (ANOVA). The experimental data on the use of BSF oil as a feed add...

Ruth Dameria Haloho1*, Jisril Palayukan1, Agus Setiadi2, Edy Rianto2, Nadlirotun Luthfi3

...cted by purposive random sampling for the highest population of buffalo. One hundred buffalo farmers were taken as respondents by a multistage random sampling method. The data were obtained by observation and direct interviews the farmers. The data was analysed descriptively and quantitatively. The study showed that there are two kinds of buffalo in West Sulawesi, the first one is the expensive buffalo and raised by intensiv...

Alif Rahman Rohim Puarada*, Safika, Agustin Indrawati

...nation mechanisms. Fecal samples were collected from Papua, Indonesia. The DNA was extracted using the DNeasy Power Soil Pro Kit. A shotgun metagenomic sequencing process from the MGI DNBSEQ-G400 platform was carried out to obtain DNA sequence data from the genes in the sample. The resulting DNA sequence data were then analyzed using MEGAN6 software using the SEED subsystem. The investigation reveals a notable abundance of A...

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...echniques. A total of 59 samples were collected from Mingaladon township, Yangon region, and the presence of infection was assessed using a Giemsa-stained thin blood smear and PCR of 16S rRNA gene amplification. Both microscopic and PCR-positive samples were further analyzed for hematobiochemical alteration. As a results, Anaplasma spp. was detected in 3.38% (2/59) of

Ida Indrayani*, Andi Andri, Tevina Edwin

 
 
... 3.19, which means it is safe for the development of ruminant livestock. Analysis of sustainability showed that the status of the economic dimension, ecological dimension, and environmental dimension were fairly sustainable, and the institutional dimension was less sustainable. 
 
Keywords | Beef cattle, Development, Dimension, Dry matter, Potential, Sustainability
...

Irfan Ullah1, Asad Ullah2*, Tahira Tayyeb1, Rafiq Ullah1, Muhammad Hanif1, Faiza Khan3, Imad Khan2, Raheela Taj4, Fatima Syed4, Shumaila Gul5, Muhammad Sadeeq6, Muneeb Islam7, Arsalan Khan8 and Khudija Ghani9

... commercial corn based basal diet (BD) with different doses of inorganic Selenium (Se) powder. Group-A was kept negative control provided with BD twice a day. Group-B was positive control group fed with 15mg DEX/kg twice/day. The Group-C was fed with BD+0.2mgSe+15mg DEX/kg. Similarly, Group-D was fed with BD+0.3mgSe+15mgDEX. Group-E was fed with BD+0.4mgSe+15mg DEX/kg. Two chicks were randomly slaughtered from each replicate and blood glucose level, kidney fun...

Abdul Haseeb1, Yasir Iftikhar1, Muhammad Ahmad Zeshan1*, Safdar Ali2, Rana Binyamin3, Salman Ghuffar4 and Muhammad Usman Ghani5,6

Moazama Batool1*, Saima Naz2*, Ghulam Abbas3, Ahmad Manan Mustafa Chatha4, Mamoona Mahmood1, Asma Aziz1 and Fatima Yasmin2

...ly (p < 0.05). At the same time, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glucose and cholesterol were significantly (p < 0.05) increased in the treated groups compared to the control group. In conclusion, the study indicates that exposure to cadmium chloride, even in a low concentration, can cause adverse hematological and biochemical changes in Labeo rohita.

...

Wali Muhammad Mangrio1*, Hakim Ali Sahito1, Faheem Ahmed Jatoi1 and Fahmeeda Imdad Sahito2

Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil

...tenuated by combining passaging with limiting 10-fold dilutions in the allantoic cavity of embryonated hens eggs for 20 passages. The attenuated virus was used to vaccinate 20 SPF chicks and its protective efficacy was checked. Intracerebral pathogenicity index (ICPI) of the attenuated strain was 0.2, the mean death time (MDT) was 120 hours and infectvity titer was 7 log10 EID50/ml compared to 1.97 ICPI, 36 hours MDT, and 9....

Diding Latipudin*, Chitra Kumalasari, Lovita Adriani

...treatment consists T0: basal rations, T1: basal rations + 100% CM dried probiotic, T2: basal rations + 75% CM + 25% fermented MBM dried probiotic, T3: basal rations + 75% CM + 25% SM dried probiotic, and T4: basal ration + 50% CM + 25% MBM + 25% SM. Each treatment was given as much as 2% dried probiotic of the total ra...

Aalaa S. Saad1*, Amira A. Elokle2, Dalia Y. Youssef2, Neven M. Omara2, Samr Kassem3

...xt-align: justify;">Chitosan nanoparticles loaded with lysozyme (CS-LZ-NPs) are gaining popularity as an alternative approach for inhibiting foodborne pathogens. This study aimed to evaluate the antibacterial activity of synthesized Chitosan nanoparticles (CS-NPs) and CS-LZ-NPs against E. coli O157 in chilled chicken fillets and relative eaeA (Intimin), stx2 (Shiga toxin) expression. The average particle size was 250 nm and ...

Sara Brikat, Mouloud Lamtai*, Miloud Chakit, Laila Ibouzine-Dine, Ilhame Fitah, Oumaima Abouyaala, Abdelhalem Mesfioui, Aboubaker El Hessni

...rcuma longa (Curc) and losartan on memory impairment induced by a high fructose diet (HFD) in Wistar rats. 36 female Wistar rats aged 2 months and weighing 74 to 100 g were randomly divided into 6 groups as follows: 2 groups would receive a standard diet which a group serve as control and a group receive 100 mg/kg/d of Curc and 30 mg/kg/d of Losartan (Curc + Los), 4 groups would receive the high HFD diet: one group serve as ...

Cut Intan Novita1,2, Kartini Eriani3, Amalia Sutriana4, Tongku Nizwan Siregar5,6*, Ni Wayan Karja7

...vent apoptosis of granulosa cell follicles. Bovine pituitary has been proven as an effective substitute for the functions of FSH and LH in some studies related to superovulation due to its high content of both hormones. Therefore, this study aimed to determine the effect of bovine pituitary extract (BPE) supplementaion to vitrification media. A total of 18 ovaries were usedwhich was divided into two groups: the vitrification medium group without Bovine Pituita...

Genlong Zhan1*, Yunhua Zheng2, Desheng Li*4 and Hongmei Zhang3

...s of simplicity and high safety.

...

Aniqa Qaisar, Shabana Naz* and Sajida Arooj

...l, water, fecal and feed samples were collected as indicators to estimate the heavy metals pollution in the environment of captive P. cristatus. Cr. Pb, Ni, Co and Mn were identified through atomic absorption spectrophotometer. The highest accumulation of heavy metals was found in feed samples followed by fecal matter as compared to water and soil samples in all the sites. Cr was found hig...

Perinçek Seçkinozan Seker

...NA belonging to the four samples of Capreolus capreolus were partially sequenced and analyzed alongside the sequences obtained from GenBank. The cyt b and COI data sets included sequences that were 1059 and 646 bp long, respectively. The Turkish roe deer population in Artvin had a relatively low nucleotide diversity and high haplotype diversity. A total of two haplotypes were identified for cyt b, while three haplotypes were identified for COI. Of the haplotyp...
Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
...m paragallinarum, the causal agent of Infectious Coryza (IC), from layer chickens in various farms in Pakistan. A total of 46 isolates were obtained from a total of 244 clinical samples from 122 sick and moribund or dead layer chickens showing signs of IC like slight swelling of infra orbital sinuses and nostril mucus membranes. Two sampling sites were chosen, squeezing the nostrils for li...
Nusrat Bano1, Muhammad Tayyab1*, Bushra Muneer2, Sehrish Firyal1, Abu Saeed Hashmi3, Muhammad Wasim1 and Ali Raza Awan1
...ia (62.5%), elevated transaminases (ALT 56.5% and AST 70.5%), hyponatremia (51.8%), hypokalemia (40.7%) and hypocalcemia (81.4%) was recorded among dengue patient. It was concluded that bleeding tendencies, retro-orbital pain, rash and vomiting were more frequent in DHF and DSS cases. Thrombocytopenia, leukopenia, deranged hematocrit, raised transaminases, urea and creatinine levels and decreased serum levels of albumin, cho...

Rahib Ali Chachar1, Asghar Ali Kamboh1* and Manatbai Bakhetgul2

... The T0 offered only a basal diet, while T1, T2 and T3 were supplemented with moringa (5%), neem (1%) and moringa (5%) + neem (1%) leaf powder respectively in the basal diet. Results showed that live body weight and carcass weight were better (p<0.05) in T3 than in control and other supplemented groups. The relative weight of the spleen was found higher (p<0.05) in T2 and T3 groups as compared to T0 and T1 groups. The ...

Muhammad Azhar1,2, Rida Ahmad3*, Bushra Nisar Khan3 and Zahra Hussain3

...on (MCHC), aspartate transaminase (AST), alkaline phosphatase (ALP), alanine transaminase (ALT), bilirubin, creatinine, blood urea nitrogen (BUN) were determined. Further, different biological, analytical and operational factors which impose significant effects on hematochemical factors were also considered to rationalize the results. Most of the values were in accordance with the mean values published in the literature, but...

Waseem Abbas1, Dildar Hussain Kalhoro1*, Hasina Baloch1,  Muhammad Abubakar3, Muhammad Saleem Kalhoro1, Neluma Wali2, Mazhar Hussain Mangi1, Azhar Ali Laghari4, Shahid Hussain Abro1, Rani Wagan1 and Mehkar Hussain5

...e linked immunosorbent assay (I-ELISA). Seroprevalence at district Gilgit was recorded 6.66% in cows and 6.66% in yak while it was 3.33% in zo. Seroprevalence at Nagar district was recorded as 43.33% in cows and13.33% in yak while it was 10% in zo. The overall prevalence at district Gilgit was recorded 5.55% and it was 22.22% at Nagar district. The overall prevalence at summer season recor...

Rogia SA Gomez*, Said H Mbaga 

...non-probability snowball sampling method. Information was collected on farmers, flocks, production costs and farm incomes. From this data, eight variables were selected and subjected to a principal component analysis (PCA), enabling three groups of farmers to be distinguished. The productivity of each group was assessed based on the economic information collected from the farmers. Overall, the results showed that the number of birds per flock was less than 100...

Fazal Ur Rehman1*, Saeeda Fatehullah1, Ayaz Qadir1, Suleman Ahmad2, Siddique Usman2, Muhammad Asif2, Mukhtiar Ahmed1, Sunaina Rafi3, Muhammad Mohsin2, Ayesha Amanullah4, Faisal Shahzad2, Hasnain Raza2 

...nd closed method of anal sacculectomy for better outcomes in respect of post-operative complications in dogs. The study was performed on 16 adult dogs of either sex or breed with 1 to 3 years of age presented at University Outdoor Clinic which required anal sacculectomy. All the animals were categorized into two equal groups i.e. group-A and group-B. Group A underwent open anal sacculectom...

Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*

...administered with normal saline (D0), a CCoV-induced group (D1), and a group induced with CCoV along with 1 mL/kg body weight of Lactobacillus acidophilus for seven days (D2). The CCoV vaccine was administered subcutaneously at 0.5 mL/kg body weight, while the Lactobacillus acidophilus isolate was given orally for seven days. On the eighth day, all mouse groups were euthanized, and tissue samples were collected for immunohis...

Huda S.J1*, Nasr T.M1, Mohanad A. Al-Bayati2

... profile after the blood samples treated period. Hemorrhagic Anemia was artificially induced in rabbits through a process of bloodletting depending on the body weight. Nano Cobalt Ferrite was prepared and encapsulated by Nano hemosome via the Bangosome method. Anemic rabbit dosed with Nano hemosomal Cobalt-Ferrite 1/40of LD50% dose 75mg/kg Bw. Hematological parameters were analyzed in the blood samples that were collected fr...

Faris Sahib Imran1*, Layth Hamzah Merzah2, Mohammed Mohsin Kareem3

...l grass grazing as the basal diet. The results revealed that weight gain, final weight, fat tail weight, hemoglobin, packed cell volume, glucose, and total protein of the experimental group was significantly (P < 0.05) higher than that of the control group A diet containing C. demersum has the potential as a protein source with a significant (P < 0.05) positive effect on the growth performance and hematological and biochemical parameters of Iraqi Awassi ...

Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee

...ys show vacuolation of mesangial cells in the glomerular tuft. Muscles of treated rats revealed severe histopathological changes under stress from both doses of toxic cholecalciferol, also showing the widespread mineralization of muscle fibers. From this study concluded that both doses of vitamin D can be toxic to organisms at higher levels, so they need to be used carefully and should not be taken excessively.
 
Keywords | Hypervi...

Moazama Batool1*, Saima Naz2*, Sheeza Bano1, Sadia Nazir3, Ghulam Abbas4, Ahmad Manan Mustafa Chatha5, Maria Lateef2 and Fatima Yasmin2

...ee replicates for each dosage in the tests. Significant variations were noted in the LC50 values and lethal responses for both fish species. Catla catla exhibited mean LC50 and lethal concentrations of 55.78±0.55 and 80.17±0.40mgL-1, respectively, while Labeo rohita showed values of 63.92±2.48 and 102.07±0.50mgL-1. Labeo rohita demonstrated higher sensitivity in terms of 96-hour LC50 compared to Catla catla, whereas Catla catla exhi...

Zaryab Murad1*, Sobia Bibi1, Shehr e Yar Ahmad1, Mohsin Ali Khan1, Rimsha Sadaf2, Mauz ul Haq1, Umair Manan1 and Muhammad Younas2

... problems as rice (Oryza sativa L.) had the potential that can adsorb a greater amount of Cd. The use of metals polluted rice as a staple food now becomes the main way for exposing human health to metals, thereby the amendment of biochar gained greater attention to sorb and control the uptake of heavy metals by rice. A pot experiment at The University of Agriculture Peshawar demonstrates biochar’s effectiveness in stabilizing heavy metals in soil and red...

Oybek A.Abduganiev1, Erkinjon B.Shakarboev2*

...2 individuals; Aidar Arnasay lake system – prevalence 0.9-27.8%, intensity of infection 1-46 individuals; Tuyabuguz reservoir – prevalence 2.1-26.6%, intensity of infection 1-38 individuals; fishing farms – prevalence 0.3-29.6%, intensity of infection 1-67 individuals; canals – prevalence 3.1-20.3%, intensity of infection 1-42 individuals.
 
Keywords | Helminths, Parasites, Predatory fish, Uzbekistan
...

Feri Eko Hermanto1, Yuli Frita Nuningtyas1, Filoza Marwi1, Fajar Shodiq Permata2, Agus Susilo1, Muhammad Halim Natsir1*

...s for fortifying the mucosal immune system in broiler chickens. However, understanding how these herbs confer protective effects remains challenging, given the intricate nature of mucosal immunity and its role in maintaining gut health. This study adopts a network biological approach to decode the complex web of protein interactions within mucosal immunity and explore the immunomodulatory ...

Shanti Fitriani*, Usman Pato, Yusmarini Yusuf, Emma Riftyan, Evy Rossi

...riocin, Pediococcus pentosaceus, natural biopreservative, fishballs, cold temperature
...

Ediset1*, Jaswandi2, Fuad Madarisa1, Amrizal Anas1, Rizqan2

...omparison, the number of samples determined by the Proportional Sampling technique is 50 breeders from 5 sub-districts in Padang Pariaman Regency. Data analysis was carried out in a quantitative descriptive manner, namely the adoption rate of A.I. was measured by the percentage of adoption, and the success rate of A.I. was measured by calculating Service PerConception (S/C), Conception Rate (C.R.), and Calving Rate (CvR). The result shows the adoption rate of ...

Nadya Indriyani1, Sri Murtini2*, Sri Darwati3, Cece Sumantri3

...DOC and 2 weeks, each 81 samples. Total Immunoglobulin yolk (IgY) observations were made using Enzyme Linked Immunosorbent Assay (ELISA) and antibody titer against Newcastle disease virus (AbNDV) methods using Hemaglutination Inhibition (HI) tests. Descriptive analysis was performed on production performance data and humoral immunity to estimate phenotyp...

Urip Rosani1*, Iman Hernaman1, Rahmat Hidayat1, Darmawan Hidayat2

...mplitude) when measuring samples, as well as the manufacture of a series of compact ultrasonic test systems so that they can be applied in industry or practically in the field.
 
Keywords | Ultrasonic Wave, Attenuation, Velocity, Rice Bran, Rice Husk
...

Kashif Abdaal, Aneeza Batool, Muhammad Tariq Navid*, Saeed Ahmed, Asma Saleem Qazi, Waseem Safdar, Haidar Ali, Mobeen Ur Rashid, Somia Rafaqat 

...dquo;. To assess vaccine safety and efficacy, scientists are now using various model animals like mice, ferrets, pigs, and nonhuman primates before using them for human trials. The other side of successful vaccine intervention, however, contains various issues that need to be rectified. These concerns primarily depend on target pathogen selection, novel vaccine efficacy, development, and duration of optimum immunological responses, selection of appropriate ani...

MUHAMMAD ASLAM1*, ZAHRA NOREEN2, AMINA ASGHAR1, ABRAR HUSSAIN2, RASHAD MEHMOOD3, MUHAMMAD USMAN KHAN4, KAYNAT SALEEM5, MUHAMMAD TAHIR HUSSAIN6* & ASMA CHAUDHARY7

... efficient one-pot condensation of 3-chlorobenzophenone and acetylacetone. Its structure was elucidated by spectral data, X-ray diffraction studies and also investigated using density functional theory (DFT). Optimized geometry was obtained by performing DFT calculations at B3LYP level of theory and 6311+G (d,p) basis set. Frontier molecular orbital analysis has been executed at B3LYP/6-311+G(d,p) level of theory. The global reactivity parameters were explored...

LAILA SHAHZAD*1, ARIFA TAHIR2 & FAIZA SHARIF3

...onditions and previous disasters history. In order to adapt to change in climatic conditions, mitigatory measures should be taken to improve their living conditions. Deforestation should also be avoided to overcome and reduce their vulnerabilities. Community resilience can help in mitigation and adaptation at local level.

...

TAHIR IQBAL1, 2, UMER RASHID1* & MUHAMMAD IDREES3, 4

...as been considered as causative agent of hepatitis-splenomegaly syndrome (HSS) in chickens. Like other HEV strains, it is single-stranded positive sense RNA virus belonging to Hepeviridae. In the present study we report partial structural homology of ORF3 encoded protein (ORF3P) of Pakistani novel aHEV (Pak naHEV) strain with a kinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophos-phokinase (HPPK) which participates in folate biosynthesis in bacter...

MAHRUKH SHAHID1, KHAJISTA JABEEN1* & SUMERA IQBAL1

... was evaluated against Fusarium oxysporum Schlecht. Methanol was used to prepare biochar extract of which various concentrations were experienced in-vitro against tested fungal strain. All the applied concentrations of methanol extract i.e. 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8% effectively inhibited F. oxysporum growth. However, 7% and 8% concentrations were proved to be most efficient in retarding the test fungus growth upto 15%. Partitioning of biochar methanoli...

ZOHAIB SAEED1, SHAHID IQBAL*1, UMER YOUNAS1,2, MUHAMMAD PERVAIZ*3, SYED MOHSIN ALI NAQVI3 & RANA RASHAD MAHMOOD KHAN3

...xt-align: justify;">Disposal of sewage sludge is a critical issue worldwide for the environmentalists. There are many methods for its disposal. The most suitable and economical method is its land application. Lagenaria siceraria L. plant was grown in various amendments of soil and sewage sludge to check the tolerance of the plant and to select the optimized mixing of sewage sludge with soil for this particular plant. Estimat...

ZARYAB KHALID SIAL & FARAH KHAN

...nverted into cDNA on the same day and amplified. The identification of PSTVd was carried out by Sequencing and BLAST. The advanced optimized Rapid Trizol reagent method was comparatively found best and reproducible as compared to the second method. The Sequencing and BLAST confirmed the presence of Pakistani PSTVd (Accession # MK.303578) in infected potato plants.

...

MADIHA AFTAB1, ARIFA TAHIR*1, TAYYABA ASIM1 & IRFANA MARYAM2

...2 medium containing g/L: sawdust, 5.0; glucose, 5.0; peptone, 6.0; MgSO4.7H2O, 0.25; KCl, 0.5; KH2PO4, 0.025. Optimized conditions for maximum enzyme yield were inoculum size 2.5%, Temperature 25˚C, pH 4.5, incubation period 7 days and moisture content 60%. Eleven different inducers were evaluated but FeSO4.7H2O showed maximum enzyme production (2156±0.57 U/mL/min). By using these statistical techniques more than 13 folds increase in enzyme yield (4022...
ZAFAR IQBAL KHAN1*, KAFEEL AHMAD1, KHALID NAWAZ2, MUHAMMAD NADEEM3, ASMA ASHFAQ1,
BABAR MUNIR1, HAFSA MEMOONA3, MADIHA SANA3, FARZANA SHAHEEN1, NAUNAIN MEHMOOD4, HIRA MUQADAS4, MAHPARA SHEZADI5, IJAZ RASOOL NOORKA6, HUMAYUN BASHIR1,
MUDASRA MUNIR1, ILKER UGULU7 & YUNUS DOGAN7
...er, soil and wheat grain samples were analyzed for heavy metals by Atomic Absorption Spectrophotometer. Concentrations of Mn (0.29-0.61), Ni (0.79-1.02), and Pb (0.01-0.42) mg/L in water samples were higher than the permissible limit of Mn (0.2), Ni (0.20), and Pb (0.1) mg/L given by FAO, while value (mg/L) of Zn (0.61-0.80 mg/L) was within the acceptable limit recommended for irrigation. In grain sa...

KAFEEL AHMAD1, NIMRA ARSHAD1, ZAFAR IQBAL KHAN1, KINZA WAJID1, HUMAYUN BASHIR1, IJAZ RASOOL NOORKA2, MAHPARA SHEHZADI3, HAFSA MEMONA4, MADIHA SANA4, KHALID NAWAZ5, MUDASRA MUNIR1, IFRA SALEEM MALIK1, TASAWAR ABBAS6, ABRAR HUSSAIN7, MUHAMMAD SHER8, MUHAMMAD FAHAD ULLAH6, MUHAMMAD AKRAM QAZI9, MUHAMMAD NADEEM10, MUHAMMAD RIZWAN QURESHI10

..., soil and buffalo blood samples to analyze heavy metals in them. Forages, soil and buffalo blood were collected from six sites of sampling. Out of which 5 sites were on the roadside and one site was away from the road. Grinding of the collected samples of soil and forage was followed by their acid digestion. Buffalo blood samples were centrifuged and th...

IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3

...sica and Trifolium honey samples against microorganisms isolated from infected burned skin of patients in children hospital, Lahore, Pakistan. The isolated microorganisms were identified as P. aeruginosa, E.coli, K. pneumoniae and S. aureus. The original bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and spread on the nutrient agar plates. Whattmann filter paper discs were soaked in three different conce...

NAILA MALKANI*, IRFAN ISHAQUE, KHALID SHAHBAZ, RIZWAN ULLAH KHAN, ARIFA SHABBIR & ATIF YAQUB

...ohita were healthier and safer to consume compared to the wild-caught fish.

...

AAMIR MAHMOOD1, ZAHOOR AHMAD SAJID* & SHEZA AYAZ KHILJI2

...to measure the impact of salicylic acid (SA) on maize under salt stress. For this purpose salinity effect on various morpho-physiological and biochemical attributes of plants was analyzed by launching a petri dish and pot experiment. The analysis spread out in a totally block design (randomized) with ten replicates for...

ARIFA ZEREEN & ALMAS JAHAN

...green belts. With the passage of time the exotic plant species also called Invasive Alien Species (IAS) is going to be a serious threat to local ecosystems. This research work was planned to determine the harmful and significant effects of exotic plants on local flora. Fourteen species of exotic and invasive plants viz., Eucalyptus camaldulensis Dehnh., Populus deltoids W. Bartram ex Marshall, Prosopis juliflora (Sw.) DC., Broussonetia papyrifera (L.) Vent, La...

KAFEEL AHMAD1, ROBINA KOKAB1, ZAFAR IQBAL KHAN1, ASMA ASHFAQ1, HUMAYUN BASHIR1, MUDASRA MUNIR1, MUHAMMAD SHER2, KINZA WAJID1, HASFSA MEMONA3, MADIHA SANA3, IJAZ RASOOL NOORKA4, MAHPARA SHEHZADI5, MUHAMMAD NADEEM6, TAHIR MAHMOOD QURESHI6, NAUNAIN MEHMOOD7, ABID IJAZ1, BUSHRA HUMA1, IFRA SALEEM MALIK1, KHALID NAWAZ8 ILKER UGULU9 &YUNUS DOGAN9

...real crops and implement safe practices for wastewater usage.

...

Wesam Jasim Hansh 

...with other international samples in the GenBank. 7 sequences of 28S rRNA gene of F. gigentica from cows were placed in the database of the GenBank with accession numbers (LC731384, LC731385, LC731386, LC731387, LC731388, LC731389, LC731390), and 10 other sequences of F.gigentica from buffaloes (LC730609, LC730653, LC730654, LC730655, LC730656, LC730657, LC730658, LC730659, LC730660, LC730661) were also recorded in this study. In phylogenetic analysis, Iraqi F....

Adriana Beatriz Sánchez-Urdaneta1,2*, Gisela del Carmen Rivero-Maldonado2, Cecilia Beatriz Peña-Valdivia3 and Dianelis del Carmen Sánchez-Urdaneta4

 

 

... arid conditions. At the same time, these characteristics can optimize the efficient use of water, while providing greater protection of the inner tissues. All of the above has applicability for the selection and cultivation of promising genotypes.
...
Jaime Vera Chang1, Arianna Torres Coronel2, Luis Vásquez Cortez2,3, Kerly Alvarado Vásquez2,3 and Frank Intriago Flor4*
 
...AT-46-88, T5-CCAT-49-98) said varieties were obtained from the Experimental Farm “ “The Dam.” For the evaluation of the physical-chemical parameters, the following variables were analyzed (acidity, °Brix, pH, potassium, fat, protein, fiber and energy), sensory variables (flavor, color, taste, smell and texture). In the analysis of variance (ANDEVA) it is concluded that in the bromatological analyzes T2 contains the greatest amount of ener...

SYEDA NADIA AHMAD1, MEHWISH NASIR1, AQSA NAUREEN1, SHAGUFTA ANDLEEB4, KAUSAR RAEES3, TAHIR ABBAS2, ASMA YOUNIS1 & KHAWAJA RAEES AHMAD*1

NUSRAT MAJEED* & ABIDA BUTT

... to provide inventory of salticids, which would be the baseline documentation for future studies in Pakistan. This survey was conducted from June 2013 to May 2017, to collect jumping spiders from twelve different localities of Pakistan. Specimens (2556) were collected which belong to twenty-eight species and twenty-one genera. This study added twenty-three first records of species from ten genera. The female of Phintella indica is in this study for the first t...

ASMA WAHEED QURESHI1* & SHUMAILA ALAM2

...racea), lettuce (Lactuca sativa), carrot (Daucus carota), mint (Mentha spicata), chili (Capsicum frutescens), cucumber (Cucumis sativus) and corriander (Coriandrum sativum) were assessed using standard methods. Overall parasitic contamination of 30.7% was observed. Highest contamination was detected in Mardan (36%) followed by Katlang (30%) while the lowest contamination was observed in Ta...

SYED SHAHID IMRAN BUKHARI1*, IQRA AZAM1, MUDDASIR HASSAN ABBASI1, MEERAB DAUD1, NADEEM SHEIKH2, ANDLEEB BATOOL3, RABIA MAHMOOD2 & MARYAM MUKHTAR2

... 6 and 12 hours. All the samples were processed for gel run and thermo cycler program, which revealed alterations in the expression of hepatic mRNA (IL-6) in all loads equally confirmed by the results of housekeeping gene β-actin. Gel analysis showed changes in the folds of IL-6 in hepatic mRNA compared to control. These finding led to the conclusion that Alloxan can induce an APR, which is evident by rise in expression of IL-6. Hence, extensive use of th...

ASIFA BASHIR1, AAMIR MUSHTAQ2* & TOOBA MEHBOOB3

...ic basis of traditional usage of Phyllanthus emblica as an anti-diabetic agent in hyperglycemic patients. Ethanolic extract of its dried fruits was analyzed both qualitatively and quantitatively for evaluation of phytochemical agents. Then its antioxidant and anti-diabetic activities were found. The results indicated the presence of carbohydrates, tannins, phenols, alkaloids, flavonoids, saponins, glycosides, amino acids and...

MUJIBUR RAHMAN1, MUHAMMAD AYAZ KHAN1*, WISAL SHAH1 & ALIA NAZ1

...urpose, soil and B. rapa samples were collected from primary, secondary, tertiary roadside fields and control site. The samples were investigated for Cd and Pb presence via atomic absorption spectrometer (PerkinElmer, AAS-PEA-700). The Cd concentrations in soil ranged from 2.55 to 4.35 with mean value 3.31 mg/kg, 3 to 8.85 with mean value 5.41 mg/kg, 3.15 to 5.55 with mean value 4.9 mg/kg and 2.45 to 5.25 with mean value 3.9...

MUSHTAQ HUSSAIN LASHARI1*, RABIA HAMEED1, UMER FAROOQ2, MUSADIQ IDRIS2 & ZIA UR REHMAN2

...t-align: justify;">Blood samples of healthy adult humans (n = 40) and dogs (n = 30) grouped as males and females, were collected aseptically through appropriate measures. Thin blood smears were stained using Field stain ‘A’ and ‘B’. The PMNs were counted and observed for Barr bodies attached to them. Only the neutrophils with terminable lobes were examined and counted. The mean value ± SE of the Barr bodies was calculated and the...

ZEESHAN REHMAN1, RANA ABRAR HUSSAIN1, SHAISTA JABEEN2, SAKHAWAT ALI2, ZAHAR NOREEN1 & IRUM MUKHTAR1*

...l Zn in sewage-irrigated samples was the highest (153.4233mg kg−1), followed by Cd2+ (70.47333 mg kg−1) and Pb (65.79667mg kg−1). Results showed higher Pb2+ and Cd2+ level in B. oleracea than daily intake of metals (DIM) standard limits, cultivated on wastewater. Whereas health risk index (HRI) was found maximum (23.22 mg kg−1) for this vegetable cultivated on waste water in Kasur region. From the health point of view, the long-term con...

MUBEEN RIAZ1, KIRAN ZAHID1, SUMAIRA ASLAM CHOHAN1, MUHAMMAD ASHFAQ*2, MUHAMMAD ALI2, RIFFAT SIDDIQUE1, NUDRAT ANEES1 & FARAH KHAN1

... plant, 02 with the 25mM salt stressed plant and 02 with the 50mM salt stressed plants. While with 75mM and 100mM salt stressed plants polymorphic bands were observed showing genetic variation. Profiles generated by the third primer i.e. OPI_05_R showed 4 monomorphic bands with control and NaCl stressed O. tenuiflorum L. While, 100mM salt stressed plant ...

SEHRISH MUSHTAQ1, MUHAMMAD SHAFIQ1, MUHAMMAD ASHFAQ*1 FAIZA KHAN1, SOHAIB AFZAAL1, UMMAD HUSSAIN1, MUBASSHIR HUSSAIN1, RASHID MUKHTAR BALAL2 & MUHAMMAD SALEEM HAIDER1

NAUSHEEN MAZHAR*, ALI IQTADAR MIRZA**, SAHAR ZIA*, ZAYANAH SOHAIL BUTT*, MEMUNA GHAFOOR SHAHID*** & ALI IMAM MIRZA**

...re also derived from the satellite images. NDSI for snow cover mapping of Passu glacier indicates a decrease of 6.18% in snow covered area (3.0808 km2) of Passu glacier from 1992-2016. This was identified as 12.8% as annual in the area of Passu glacier. In response to this glacial retreat, area under vegetation has increased @ 4 percent per annum since 1992 and it covers almost half of the area (0.981 sq. km area) of Passu village which is fed by the melted wa...

*SHAJAHAN BAIG1, MUSHTAQ AHMAD SALEEM1, MUHAMMAD AZMAT ULLAH KHAN1 ZEESHAN NADEEM2, SUFIAN AHMED2, MEMUNA GHAFOOR SHAHID3 & TANZEEM AKBAR CHEEMA3

...g methods to analyze the safety of genetically modified (GM) foods. These bioinformatic methods employ the comparison of different linked databases which may cover all the information significant for profiling. These methods are also significant in identifying the changes at various stages of development under diverse environmental conditions.

...

ABRAR HUSSAIN1, AQSA KHALIL1, SAIRA ZAHEER1, MUHAMMAD LATEEF2 & MUHAMMAD MANSHA1

...epared. Adult motility assay (AMA) was carried out on adult Haemonchus contortus to analyze the anthelmintic efficacy of CEE of the whole plant and the leaves of Tribulus terrestris L and Ziziphus mauritiana Lam. The motility of insects was assessed at concentration of 2, 4, 6 & 8 mg/ml after 2, 4, 6 & 8 hours of post treatment and dead worms were noted. The 8mg/ml concentration of CEE of Tribulus terrestris L. and Ziziphus mauritiana Lam. exhibited 90...

ASADULLAH KHAN1, MUHAMMAD AYAZ KHAN1, ALIA NAZ1, ABDULLAH KHAN1 & NAUREEN AURANGZEB1

...ocarpon miniatum species samples were collected from control site, primary, secondary and tertiary roads and were investigated for Pb and Cd concentrations and reduction in chlorophyll contents. The data were analyzed using ANOVA, correlation and regression analysis. Significant (P<0.05) variation in Pb and Cd concentrations in lichens along different roads were found. It was concluded that traffic density was responsible for Pb and Cd accumulation in liche...

*ARIFA ZEREEN1, ALMAS JAHAN1,SHEIKH SAEED AHMAD2 & ZAHEER-UD-DIN KHAN3

MUSHTAQ HUSSAIN LASHARI1*, UMER FAROOQ2, AMMARA ARSHAD1, MUHAMMAD NAEEM TAHIR2, MUSADIQ IDRIS2, ANAM KHURSHID3 & ZIA UR REHMAN2

...attle and buffalo. Blood samples of adult cattle (n=26) and buffaloes (n=26) grouped as males and females (n=13 each), were collected from the jugular vein through appropriate measures. Thin blood smears were stained using Field stain „A‟ and „B‟. The PMNs were counted and observed for Barr Bodies attached to them. Only the neutrophils with terminable lobes were examined and counted. The mean value ±SE of the Barr Bodies was calculated a...

SIDRA MOQADDES*¹, RABIA ZULFIQAR1, MAWRA GOHAR1 & HINA QAISER1

...udy revealed that 61% of samples were positive for Escherichia coli, other contributing pathogens were Staphylococcus spp. (24%), Klebsiella spp. (9%), Proteus spp.(4%) and others (2%). Out of 85 samples (march-june 2015), 48(56%) were from females and 37(44%) from males. Samples were categorized into five age categories age and majority of the collected samples (42%) were found in age gro...

ZAFAR IQBAL KHAN1, KAFEEL AHMAD1, HAREEM SAFDAR1, KINZA WAJID1, HUMAYUN BASHIR1, KHALID NAWAZ2, MAHPARA SHEHZADI3, IJAZ RASOOL NOORKA4, MUDASRA MUNIR1, IFRASALEEM MALIK1, ASMA ASHFAQ1, HIRA MUQADDAS5, HAFSA MEMOONA6, MADIHA SANA6, NAUNAIN MEHMOOD7, SAIF UR REHMAN8 & TASAWAR ABBAS8

...n water, root and forage samples was determined. Moreover, bioconcentration factor, pollution load index, daily intake of metals and health risk index were calculated. In tap water Cd value was 0.54 mg/L and in sewage water, it was 0.56 mg/L. In soil the calculated Cd value was lower than USEPA standards. The maximum Cd in root was calculated in winter forages (1.15 mg/kg). The calculated value for Cd in leaves was higher than the maximum permissible limit 0.5...

KOUSAR PERVEEN1*, MUHAMMAD AFZAL1, SANA SEHAR1 & SYED AMIR GILANI2

...de medical services that satisfy a patient both physically and mentally. The state of the art medical facilities may endow patients‟ physical health but patients‟ emotional well-being depends on how healthcare personnel treat patients during the curing process. In this regard, the extant literature suggests that compassionate care behaviour of healthcare personnel plays the leading role. However, scant attention has been paid to understand the phenomena of...

HANNANA MARYAM, SANA MAQSOOD & SIKANDER ALI*

... in liquid culture. Soil samples were collected from different habitats of Lahore(Pakistan) for the isolation of bacterial cultures. After primary and secondary screening, ISL-4 (0.53 IBU/ml) and ISL-7 (0.6 IBU/ml) were selected having better potential for bacteriocin. A significant (p≤0.05) increase in laterosporulin biosynthesis was observed when the parameters like medium volume, buffer pH (7.0) and incubation temperature (37°C) were optimized. Antim...

LAILA SHAHZAD*1, MUHAMMAD UMER HAYYAT1, NIDA SAJID1, FAIZA SHARIF1, ASMA MANSOOR1, MANAL SHAH1 & NABEEHA LODHI1

...lnerability to natural disasters and the related level of awareness among community. A household questionnaire based survey was used to determine awareness and resilience of the community. Another methodology named Rapid Visual Screening (RVS) was used to determine the factors that contribute to infrastructure vulnerability to natural disasters. Factors assessed through this methodology are vertical and plan irregularity, so...
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
...olic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans, Fusarium oxysporium and Microsporum canis) to check their antimicrobial activities. For antibacterial assays, the volume and concentrations of extracts used were 0.5 μl (62.5 μg), 1 μl (125 μg), 5 μl (625 μg) and 10 &m...

ZAHRA NASEEM1, MUHAMMAD AMIR IQBAL1, SHAAF AHMAD2 & NABILA ROOHI1*

...zed through Radioimmunoassay (RIA), whereas, levels of AST, ALT and creatinine were assessed using chemistry analyzer. Levels of ALT and AST were found significantly increased (P ≤ 0.05) in overt hypothyroid patients compared to controls and after treatment the concentrations of both enzymes were decreased non-significantly in post therapeutic group. Subclinical group also exhibited non-significant elevation in ALT and AST levels. Creatinine levels were sig...

ZUBIA RASHID1, ZULFIQAR ALI MIRANI2, SITWAT ZEHRA1, SYED MUDDASSAR HUSSAIN GILANI3, ARIF ALI CHISHTI1, ABID AZHAR1 & SADDIA GALANI1*

...xt-align: justify;">Food safety and quality have been a significant and crucial predicament recently. In poultry, development of novel antimicrobial strategies for drug-resistant pathogens is desired. Here, Phytogenic Feed Additives (PFA) were administered in chicken diet to decipher their impact on animal performance and gut microbiota composition. One-hundred-fifty-day-old chicks were randomly divided into three treatment groups with five replicates/group as...

MUHAMMAD JAVAID AFZAL*1, FARAH JAVAID2, SHAHZADI TAYYABA3, ANEEQA SABAH4 & MUHAMMAD WASEEM ASHRAF5

... close settlement at the same Reynolds number and pressure. This study is very important for examining the blood flow rate with blood velocity. This simulated research is helpful in finding the cure for diseases.

...

UROOJ SAEED1, SAJID RASHID AHMAD2, HAMMAD GILANI3*, RAB NAWAZ4, NAEEM SHAHZAD5, IRFAN ASHRAF6 & WAQAS AHMED QAZI3

...le Earth high-resolution satellite images, healthy growth of mangroves plantation was observed on previously barren mudflats. Even the canopy of sparse mangroves trees became enriched in various areas. In a small area of 372 km2 around the plantation sites, the study quantified 0.44 km2/year gross rate of afforestation using Normalized Difference Vegetation Index (NDVI) values derived from 30 m spatial resolution Landsat

Uqba Mehmood

...a replacement in AGFK is satisfactory and they trigger germination in germination defective bacterial spores as do asparagines. In present studies all the germination defective spores showed very low proteolytic activity in comparison with proteolytic activity of normal spores of wild type Bacillus subtilis PY79. It appears that defective spores lack enzymes with proteolytic activity which is necessary for germination. Since...

ZAHID FAROOQ1, IRFAN BABOO2, MUHAMMAD WAJID3, HAFIZA SADIA4, MUHAM

...0 male, 18 female) blood samples of healthy birds were collected from four different captive facilities under the control of Punjab Wildlife and Parks Department, Government of Punjab, Pakistan. Hematological parameters including Total Red Blood Cell Count, Total White Blood Cell Count, Packed Cell Volume, Hemoglobin, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration, Platelets, Lymphocytes, Monocytes and Neutrophi...

ALIA NASEEM, *SUMERA IQBAL & KHAJISTA JABEEN

...eated and untreated soil samples were amended with compost and farmyard manure at 2.5 % and 5 %. It was demonstrated that in okra when compared with control, the sugar content and germination parameters were affected adversely by Cr application. The treatments of compost at 5 percent and farmyard manure at 2.5 percent positively affected the yield in both Cr treated and untreated plants. In comparative analysis compost appeared as more beneficial for okra than...

MARIAM ZAHEER*1, SYED SHAHID IMRAN BUKHARI2, MUDDA

...-align: justify;">Exopolysaccharides (EPSs) are secreted by both eukaryotes (fungi, phytoplankton, and algae) and prokaryotes (archaeobacteria and eubacteria). EPSs produced by bacteria include a broad range of chemical structures and may either be heteropolymeric or homopolymeric. Their molecular weights vary from 10 to 1000 kDa. Exopolysaccharides have multifarious applications in different fields of life. These EPSs are u...

UMER YOUNAS, SHAHID IQBAL* & JAMSHED AKBAR

...been studied. Initially, samples were dried using different drying procedures (shade, oven and microwave drying) and extracted using different solvents (ethyl acetate, acetone and methanol) in combination with microwave-assisted extraction technique. Antioxidant potentials of extracts were determined in terms of total phenolics, total flavonoids and ascorbic acid contents. In addition, radicals (DPPH• & ABTS•+) scavenging potentials were also det...

MUHAMMAD NAEEM RAZA1, KALSOOM SUGHRA1* & NADIA ZEESHAN1

...in Gujrat, Punjab. Blood samples of 3540 suspected patient were collected from July 2017 to January 2019 and analyzed for HCV infection by using qualitative PCR, confirmed cases were subjected to molecular genotyping on Real Time PCR (Applied Biosystems, Step One software). Results show 2750 out of 3540 (77.6%) suspected patients were positive for HCV-RNA. Female have higher percentage (57.78%) than male (42.22%) with highest viral load observed at age group 1...

MUHAMMAD ASLAM1*, MISBAH IRSHAD1, AMINA ASGHAR1, ASAD GULZAR1, ZAHRA NOREEN2, ABRAR HUSSAIN2, SHAHID MASOOD3, MUHAMMAD KAMRAN SHAHID1, RASHAD MEHMOOD4, KAYNAT SALEEM5 & SAJID IQBAL1

...packs of anyone selected sample and consequently would have no negative impact on health.

...

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

..., synthesis of secretory saccharides, bioaccumulation and directed flagella based shifting resulting out of hypo-doses of drug stimulation. Further, the prevalence of Enterobacteriaceae member strains has been witnessed producing Extended Spectrum β-Lactamases (ESBLs) and Carbapenemases. Drug resistance in viral entities has also posed challenge for public health programs. About 20% people die of viral hepatitis in one of Pakistan provinces. A counter mal...

MUHAMMAD NADEEM1, MUHAMMAD WASEEM MUMTAZ1&MUHAMMAD DANISH1

...ha;-amylase inhibitory assay (IC50=42.91±1.24 μg/mL) was also depicted to be highest in the same extract. Based on current findings, 60% ethanolic extract of V. negundo leaves was revealed to be most potent for its antioxidant and α-amylase inhibition activity, hence could be used for formulation of new plant based functional food sornutraceuticals to improve human health.

...

ASUMAN ARSLAN DURU1*

...he birds were fed with basal diet supplemented with 1 g/kg (GBL1), 5 g/kg (GBL5), 10 g/kg (GBL10), 20 g/kg (GBL20) of goji berry leaves and negative control. These mentioned doses were added to per kg basal diet (166 g crude protein and 2821 kcal ME kg-1). Feed was offered limited (110 g/hen) and water was available ad-libitum. The trial was continued for 8 weeks. At the end of the study, egg yield, egg mass, feed intake and...

UZMA PARVEEN1, MASROOR ELLAHI BABAR1*, NIDA BABAR2, TAHIRA MUGHAL3 & AKHTAR ALI1

...lpha. We collected blood samples of 70 patients out of which 50 ladies gave untimely births and 20 ladies gave typical births. DNA extraction technique was applied. Primers were designed, amplified by PCR and RFLP were performed to identify the TNF–alpha -308 and -238 polymorphisms. The frequencies of TNF-alpha -308 genotype TNF GG, GA and AA were 37%, 10% and 3% with measurably estimation of 0.07039 (P<0.05) as indicated by HWE for the preterm and po...

*AMNA SHOAIB, AROOJ SHEZAD, ARSHAD JAVAID, SUNDUS AKHTAR & ZOIA ARSHAD AWAN

...ae member i.e. Rhaphinus sativus L. and three species of Trichoderma (T. harzianum, T. viride and T. hamatum) were evaluated for their antifungal effect against Sclerotium rolfsii, the causal agent of collar rot disease in chickpea. In vitro, methanolic leaves extract of R. sativus significantly decreased pathogen biomass by 6-96% and three Trichoderma species also caused significant inhib...

Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1

...ches and collected sperm samples were diluted in the ratio of 1:30 fold media and divided into different aliquots before applying them to in vitro fertilization. It was clear from the results of the current study that, the commercially available activation media C showed a highest significant effect on percent sperm motility i.e., 89.66%±2.88, motility interval 300sec±2.67%, Viability: 74.48±0.39% and fertilization rates: 90.04±1.70...

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

...n was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically and microscopically. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk and the addition of 7% DMSO cryoprotectant. Then the semen was equilibrated at different times and then ...

Karima Akool Al-Salihi1*, Luay Jumaah Jihad2, Abbas Najm Aldin Saleh3

Bela Putra1*, Budi Prasetya2

...application of a 15 Gy dosage significantly enhanced the absorption of P (p<0.01), N (p<0.01), and Ca (p<0.01) in the plant. Additionally, gamma irradiation at a 25 Gy dosage demonstrated a notable effect, resulting in the highest calcium (Ca) content (p<0.01). However, the treatment did not exhibit a significant impact on pH (p > 0.05) in the rumen fluid, while NH3 (p<0.01) and VFA (p<0.01) parameters i...

Huda K. Khassaf1*, Zainab A.H. Al-Mousawi1, Sawsan A. Ali2, Anwar Nather Seiwan3

...ha-lipoic acid (ALA) and salbutamol (SAL) on many blood thyroid hormones and electrolytes. A total of forty rats, weighing between 200-250 g at the beginning, were divided into three experimental groups at random: the control group, the SAL group which was given (80 mg/kg) of

Tri Anggraeni Kusumastuti1, Ikuo Kobayashi2, Ahmad Juwari3, Lovin Dika Antari4*

...rding to the World Organisation for Animal Health or Office International Des Epizooties (OIE). The last case of the spread of FMD in Indonesia occurred in 2022 and Japan in 2010. This research aims to determine the value of economic losses based on the conditions of beef and dairy cattle farms in two regions, namely East Java Province and Miyazaki Prefecture based on the consideration that they are cattle production centers. SWOT analysis is used to support a...

Jacob Situmeang1, Lovita Adriani1*, Deny Saefulhadjar1, Safri Ishmayana2

...tment consisted of T0: basal diet, T1: basal diet + 2% probiotic powder B1 (Bifidobacterium spp. and L. acidophilus), T2: basal diet + 3% probiotic powder B1, T3: basal diet + 2% probiotic powder B2 (L. bulgaricus, S. termophilus, L. acidophilus and Bifidobacterium bifidum), and T4: basal ration + 3% probiotic powder B...

Ngoc Tan Nguyen1*, Thi Tuong Vi Trang1, Thi Thuy Tien Nguyen2, Tuan Thanh Hoang2, Duc Thoa Nguyen2

...tracted from whole blood samples of 393 VLV chickens. The 24 bp indel/PRL target gene was amplified by PCR and the product was separated by electrophoresis on 2% agarose gel for genotyping. Polymorphism was observed at the 24 bp PRL/indel locus, showing two alleles I and D with frequencies 0.57 and 0.43, respectively and three genotypes (II, ID, and DD) with frequencies 18.3, 49.4, and 32.3%, respectively. Hens with the DD genotype had a significantly (P<0....

Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3

Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2

...tive ovaries and below basal level (<1ng/ml) plasma progesterone concentration. In conclusion, there was a clear seasonality pattern of reproduction in buffaloes in subtropical regions of southern Nepal with the autumn being the good and the spring being the low breeding as well as calving seasons; and 53% of buffaloes during low breeding season were in true anestrous condition.
 
Keywords | Buffalo, Reproduction, Seasonality, E...
Aliya Irshad Sani1, Shumaila Usman2*, Zile Rubab1, Sufyan Ahmed3, Sana Iqbal3 and Zehra Ahmed4
...ll carcinoma patients in saliva and blood as noninvasive biological samples by qPCR. The results revealed significant difference between the cycle threshold (Ct) values in the saliva and blood of OX40 (p value=<0.001) and OX40L (p value=<0.001) in OSCC patients. The fold change overexpression in blood and saliva is also compared in relation to hist...

Anisa Mushtaq1, Murtaz ul Hasan1*, Asim Shamim2, Muhammad Ali Abdullah Shah1, Muhmamad Arif Zafar3, Abdul Asim Farooq4, Aayesha Riaz1, Muhammad Kamran1 and Saif ur Rehman

...um excavatum and Haemaphysalis punctata. Of those four species were confirmed on morphological basis, two ticks species Rhipicephalus microplus and Hyalomma anatolicum anatolicum whose morphological feature were overlapping with other identified species, were confirmed through molecular tools amplifying ITS-2 gene. Ticks DNA were then examined by PCR employing a genetic marker that target (18S rRNA gene), for the presence of Babesia and Theileria species in id...

Youfang Li1, Xu Li2, Shaik Althaf Hussain3, Jayasimha Rayalu Daddam4 and Zhigang Chen5*

...3-7 animals were fed the same diet as group 2 with gentamicin administration and administered with standard drug i.e., allopurinol, EGCG, quercetin and EGCG: quercetin (1:1). All the study animals were sacrificed after 4 weeks of treatment after a 24-hour urine collection, and blood and kidneys were removed for urine and serum biochemical analysis, tissue anti-oxidant, miR-21 as well as morphological examination. We found th...

Firda Arlina1*, Mangku Mundana1, Linda Suhartati1, Agmelia Jasfaria2

...sed a survey method, and sampling was carried out using purposive sampling. The parameters observed were qualitative and quantitative phenotypes. The data obtained were analyzed by descriptive statistical analysis. The results showed that the qualitative variety of the Randah Batu Kokok Balenggek chicken reared by the Kokok Balenggek chicken Farmers in Solok Regency, West Sumatra, based on the Biriang color of 48.33%, the ye...

Muhammad Yusuf1*, Abd Latief Toleng1, Sahiruddin1, Masturi1, Hasrin1, Musdalifa Mansur2, Hendrikus Fatem3, Johan Koibur4

...g Centre (MBC) Farm of Hasanuddin University. A total of 18 dairy Holstein Friesian heifers were used in the present study. The heifers were then divided into two groups based on different treatments of estrous synchronization. All heifers were examined for ovarian activities prior to synchronize the estrus then subjected to either Heasynch or Select Synch protocols. The results of this study showed that inactive ovaries of dairy heifers were relatively higher...

Ebban Bagus Kuntadi1, and Amam Amam2*

...analysis and independent sample t-test using SPSS 26.0 software. The results revealed that the weight loss of the imported cattle on the MV Diamantina ship was 12.92 ± 5.66 kg per cattle, Barkly Pearl was 9.98 ± 5.05 kg per cattle, and MV Gudali Ex was 14.32 ± 4 .20 kg per cattle, while the weight loss of Brahman Cross heifers was 12.71 ± 6.19 kg per cattle, of steers was 12.15 ± 3.86 kg per cattle, and of bulls was 15.86 &pl...

Srisukmawati Zainudin1,3, Hartutik2, Edhy Sudjarwo2, Osfar Sjofjan2*

...ments consisted of T0 (basal feed), T1 (basal feed with 10% fresh skipjack tuna offal meal), T2 (basal feed with 10% steamed skipjack tuna offal meal), and T3 (basal feed with 10% fermented skipjack tuna offal meal). Data were collected and analyzed using analysis of variance (ANOVA). The results showed significant differences (p < 0.05) in nutrient d...

Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2

...s diluent has almost the same capabilities as Andromed ® commercial diluent. The ability to maintain cell membrane integrity is indicated by the RR value reaching 77.99%. In conclusion, Supplementation of myrmecodia extract in 2 mL of egg yolk Tris diluent affected sperm protection during the equilibration and cryopreservation processes, following Indonesian National Standard 4869-1:2021 frozen bovine semen. 
 
Keywords | ...

Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1 

...onfirmed utilizing a transabdominal probe and real-time B mode ultrasonography. The results showed that group B had a significantly (P<0.05) higher conception rate (50%) than group A (30%). In conclusion, supplementation of TEY extender with 2 mM selenium improved in-vitro (chilled and frozen-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen. 

...

Teedzai Chitura*

...suring the production of safe meat and other poultry products. It was hypothesized that probiotic supplementation can successfully replace the use of antibiotics as growth promoters in poultry diets without causing adverse effects on growth performance and products quality. A literature search was conducted to cover the scope of growth performance and meat attributes of poultry species fed diets supplemented with probiotics. The search process was conducted wi...

Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei

...otoxin. All animals were sacrificed at the conclusion of the experiment (90 days) in order to conduct biochemical, histopathological, and Immunohistochemical analyses. The oxidative stress result showed elevation in Malon Di-Aldehyde concentration in liver tissue treated with tetrodotoxin. The pathological lesion in liver of animals exposed to toxic dose of tetrodotoxin was characterized by dilation and congestion of portal vein, Focal aggregation of mononucle...

Shama Sadaf1*, Ayesha Saeed1, Komal Hassan1, Zeeshan Ahmad2 and Maha Naeem Butt1

...eam soaping machine. The sample were died then rinse the sample and dry it under shadow to penetrate color. Then the sample was washed with tap water for 2 minutes to remove extra color. After this wash all the dyed samples in machine. Put 10 litter water and 50gm detergent and wash for 15 minutes. Same procedure would be followed in level 2 with 2 table...

Bilal Saeed Khan1*, Muhammad Aneeb Shahzad1, Muhammad Irfan Ashraf2, Zahid Mahmood Sarwar3, Muhammad Farooq4 and Awias Rasool5

...was used to conduct bioassay and this experiment was performed under Complete Randomized Design (CRD) with three replications. The results showed that N. tabacum extract was more toxic to aphids, two-spotted spider mites, ladybird beetles and predatory mites and caused 80.82, 81.49, 39.77 and 51.55% mortalities, respectively, after 96 hours. M. azedarach extract showed the second highest mortalities i.e., 73.85% in aphids, 70.54% in two-spotted spider mites, 3...

Habibeh Jabbari

...evealed by soil and root samples in the vegetable growing farmlands in Tabriz, Iran. Different populations of H. cruciferae were collected from the region and characterized based on morphological, morphometric and molecular features, four out of those populations isolated from the rhizosphere and roots of four different cabbage cultivars were subjected to further studies. All the populations showed same range of morphometric...

Shahabaz Ul Haq1, Khurram Ashfaq1, Arsalan Khan2*, Adeel Khalid1, Shahrood Ahmed Siddiqui3,4, Raheela Taj5, Asad Ullah6, Hidayatullah Soomro7 and Muhammad Wasim Usmani8*

...he goat population of Faisalabad, Pakistan. A cross-sectional study was conducted at the OPD clinic of the University of Agriculture, Faisalabad, over a one-year period in 2018. Sixty goats, comprising clinically diagnosed cases of udder edema and healthy controls, were enrolled. Data were collected through structured interviews and physical examinations by veterinary professionals. Stratified random

Waseem Abbas, Imtiaz Ahmed* and Imran Khan 

...and composite test bread samples were performed using standard methods. In-vitro starch digestibility and total starch were determined by Megazyme starch assay kit. A nine-point hedonic scale assessed bread samples for consumer acceptance. The findings of proximate composition showed a significant increase in ash at 2 and 3 % incorporation. The fiber content also significantly increased at...

Mehwish Zafar1, Muhammad Shahzad1*, Muhammad Zubair Akram2, Quratulain3, Mehak Shehzad4 and Samreen Nazeer5*

...ign: justify;">Elevating salinity level has become a worldwide threat to agricultural lands. High level concentration of salts hampers growth of plants and its yield of biomass by impacting major physiological mechanisms i.e., ionic, oxidative and osmotic stress. One possible and climate resilient strategy is to introduce new crops that can bear high level salinity and allow irrigation wit...

Safdar Hassan1, Shaukat Ali Bhatti1, Fawwad Ahmad1, Asad Ullah Hyder1, Muhammad Arslan2, Ashar Mehfooz3, Ijaz Saleem3, Muhammad Umar Yaqoob4,5, Mushrraf Nazir1, Muhammad Sharif1* 

...

Broilers may not have same dietary zinc requirements under temperate climate conditions. This experiment was conducted to evaluate the effect of super dosing of dietary Zn (above National Research Council recommended: 40 mg/kg) on broilers’ growth performance, carcass characteristics, and immune response under raised temperature. A basal corn-soy diet (Zn-40, crude protein (CP): 20%; metabolizable energy (ME) : 30...

Tianhoun Denté Fidèle1,2*, Meda Nãg-Tiéro Roland2, Zabré Geneviève3, Koama Benjamin2, Kaboré Adama1, Tamboura H. Hamidou1, Bélem Adrien Marie Gaston4 

...udy was carried out to assay total phenolics and evaluate the in vitro anthelmintic efficacy of aqueous and hydroacetone extracts of Combretum micranthum leaves on the parasite Haemonchus contortus. Following phytochemical assay, five increasing concentrations (0.6; 1.2; 2.4; 4.8 and 9.6 mg/ml) of the two extracts were prepared and tested for egg hatching and larval development in the presence of positive (Albendazole) and n...

Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4  

...od, a total of 419 serum samples (227 layers and 192 broilers) were randomly collected from apparently healthy, sick and recovered birds. The haemagglutination inhibition (HI) test was used to analyze the chicken sera samples for determination of the titer of the antibodies against NDV. The test results showed that the overall seroprevalence was 13.84% (n = 58/419). Layer chickens (17.62%) were more prevalent for ND than bro...

Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4  

...od, a total of 419 serum samples (227 layers and 192 broilers) were randomly collected from apparently healthy, sick and recovered birds. The haemagglutination inhibition (HI) test was used to analyze the chicken sera samples for determination of the titer of the antibodies against NDV. The test results showed that the overall seroprevalence was 13.84% (n = 58/419). Layer chickens (17.62%) were more prevalent for ND than bro...
Samia S. Alkhalil1, Faisal Al-Sarraj2, Abeer S. Aloufi3, Zuhair M. Mohammedsaleh4, Mamdoh S. Moawadh4, Fayez M. Saleh5, Majid Al-Zahrani6, Waheeb Aggad7, Youssef S. Alghamdi8, Mutaib M. Mashraqi9, Saleh Alshamrani9 and 
Mona H. Soliman10,11*
...ol of aflatoxins is necessary to avoid the health hazards in human and animals. Present study was designed to evaluate the efficacy of Bacillus licheniformis as a novel feed additive for control of aflatoxicosis. A total of 40 rats were equally divided into the four groups. The first group (BD) was kept as control, treated with basal diet. In second group (AF), the rats were treated with aflatoxins (28 µg/kg BW). In th...

Mubashar Hussain*, Takhmina Nazir and Muhammad Faheem Malik

...ellid beetles from seven sampling sites (Behram, Wazirabad City, Khanki, Burj Cheema, Tahli da Kot, Rasool Nagar and Kot Har Auddin) by handpicking and sweeping aerial net fortnightly during 2016-2017. A total of 4589 individuals were recorded that belonged to 13 species representing six genera, three tribes and three subfamilies. The results showed that maximum number of species belong to genus Epilachna (five species) and Coccinella (four species) which make...
Muhammad Atif Raza1, Muhammad Tariq Javed2, Muhammad Fiaz1*, Muhammad Shakeel3, Muhammad Shahbaz Ul Haq2, Amna Kanwal2, Syeda Maryam Hussain1 and Muhammad Zubair Siddiqi4

Kiran Khan1*, Ata ur Rehman2, Jai Dev Maheshwari1, Ghazala Nasim Pasha3, Tayyaba Mumtaz4 and Ruqaya Nangrejo5

...8 and 35. A total of 400 samples were randomly selected from various parts of Karachi. The data was collected between February and March utilizing Google forms as a web-based questionnaire. The questions in the study included excessive smartphone use on day to day basis that was causing referred discomfort in the upper extremities in the previous 30 days. Our findings indicate that as time spent on the smartphone increases, so do problems with the upper extrem...

Mercy Cuenca-Condoy1*, Lourdes Reinoso-García2, Juan González-Rojas2, Dionel García-Bracho3  

...-Ecuador canton. One thousand four hundred and forty mammary quarters were analyzed, coming from 360 Holstein cows in milk production. The California Mastitis Test (CMT) was used to qualitatively verify the increase in somatic cell count (SCC) in milk to indicate the presence of microorganisms causing bovine mastitis. Of the 1440 quarters studied, 175 were found positive by CMT, these were plated in duplicate and incubated at 37°C for 18 hours. The microo...

Nadia Kèmi Assana Chabi1, Gildas Codjo Tchemadon1,2*, Olaïgbé Lydie Hounkpatin3, Paul Kouété Jimmy4 and Leonard Antoine Chaffara Afouda1

...ion in Benin, it is necessary to carry out in-depth studies on the distribution and importance of these diseases, set up selection programs focused on resistant varieties to viruses and improve the agricultural practices of farmers.

...

Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain

...abdoviridae and genus Lyssavirus. It is a neurological infection that is transmitted through animals and can be lethal. It is a disease that affects all warm-blooded species and is widespread worldwide, except for islands like Australia and Antarctica. Rabies causes more than 60,000 deaths annually, although every year about 15 million people receive Rabies Post-Exposure Prophylaxis (PEP). Wildlife including raccoons, skunks, bats, and foxes are major rabies r...

Muhammad Shuaib1*, Abdul Hafeez1, Woo Kyun Kim2, Aamir Khan3 and Abubakar Sufyan4

...he following groups. A basal diet of the corn-soybean meal was formulated as a control group and a soybean meal in the basal diet was replaced with 3%SH, 6%SH, and 9% SH respectively. The results of the feces proximate analysis showed significantly higher crude protein during phases 1 and 2, while crude fiber during phase1 and 3 for the SH treatment groups. The crude fat had a significantly higher value for the control group...

Yousef Abdal Jalil Fadladdin1, Ateeq Ullah2*, Raheela Nawaz3, Yagoob Garedaghi4, Abbas M.A. Al-Azab5 and Mashael Abdullah Aldamigh6

...es assessment. The stool samples were collected and preserved in 10% formalin solution and safely transported to Parasitology Laboratory, University of Malakand for parasite examination. Each of the samples was processed in direct smear methods and examined under microscope first under low power objectives and then higher power Lense. Evidence of infection was noted by the presence of helm...

Muhammad Usman1*, Aneela Zameer Durrani1, Nasir Mehmood2, Muhammad Hassan Saleem1 and Mamoona Chaudhry3

...h, 21st, and 28th. Serum samples were also collected on the same days for determining C-reactive protein (CRP) values. The results of clinical biomarkers showed that doxycycline cured the dogs immediately when checked on the 7th day followed by amoxicillin on the 21st day; whereas dogs treated with azithromycin and clindamycin did not show promising results. Besides, results of CRP showed that doxycycline had significantly (...

Xin Niu1, Xuelan Fang2, Sang Ba3, Yihao Fang1,4, Davide Fornacca1,7, Kun Tan1,4, Yanpeng Li1,4,5*, Zhipang Huang1,4,5* and Wen Xiao1,4,5,6,7

...species appearing at the same plots and the number of individuals were calculated for each site and differences were assessed according to seasons and elevation. No direct encounters between the two species were captured by camera traps, although they did appear up in the same camera traps at altitudes between 2,900 m - 3,500 m. M. mulatta was also observed at elevations below 2,500 m, near the farmland and villages. The two...

Mirwaise Khan1, Rubab Maqsood1, Hamad Bin Rashid2, Shakera Sadiq Gill1, Muhammad Noman1, Rafia Akram1, Muhammad Sajid Hasni1, Haroon Rashid3 and Mamoona Chaudhry1*

...17. A total of 240 blood samples from backyard chickens were collected and were tested for antibodies against Avian Influenza Virus (AIV) (H9N2) by hemagglutination inhibition test according to OIE. It was found that out of 240 collected blood samples, 140 (58.3%; 95% CI: 50.43-69.60) were positive for AIV (H9N2). The findings of the present study indicated that AIV (H9N2) were endemic and widely distributed in backyard poul...

Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4

...ed from peripheral blood samples using magnetic-assisted cell sorting (MACS) or by flowcytometry-mediated cell sorting. There are no reported studies from South Asia in which CD34+-derived EPCs have been isolated and purified from the peripheral blood samples obtained from healthy adult humans. The aim of the present study was to isolate CD34+ cells, differentiate CD34+ cells in to EPCs, purify and characterize CD34+-derived...

Muhammad Adnan1*, Muhammad Nauman Khan2,3, Barkat Ullah2, Faisal Zaman2, Alevcan Kaplan4, Hubert O. Dossou-Yovo5, Sajid Ali Khan Bangash6, Sana Wahab7, Mehreen Ghazal8 and Muhammad Hassan Sarfraz9

...he pots were filled with sandy loam soil (1 kg). The pots were pre-treated with boron as boric acid (H3BO3) only once before sowing, at the doses of 0 mg/kg (control), 3 mg/kg, 6 mg/kg, 9 mg/kg, 12 mg/kg, 15 mg/kg and 18 mg/kg soil. The different concentrations of Boron (B) were prepared separately by taking a respective amount of boric acid. Pots without supplemented B constituted the control. No additional supplements were applied to the experimental soil. E...
Abdul Asim Farooq1,2*, Muhammad Arif Khan1, Hamid Akbar1
Muhammad Ashraf3, Saima Inayat4, Muhammad Usman Saleem5
Saeed Murtaza2, Maqbool Hussain Shah2 and Muhammad Arshad Javid5
...on metacarpal and metatarsal fracture of Beetal goats presented at the surgery clinic of the University of Veterinary and Animal Sciences Lahore, Pakistan. Beetal goats were selected (n=20), and divided into four different groups. The first group was designated as bone plating with bone marrow aspirate (BPMA) in which fracture was reduced by using the bone plates along with the application of multiple BMA on days: 0, 14, 28, and 45. Furthermore, the second gro...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
...cts. Blackseeds (Nigella sativa) and honey possessing potent neuroprotective, antioxidant, and anti-inflammatory properties with no reported side effects can be a prospective candidate for an alternate remedial treatment of MS in animal model as well as in humans. In this study we established a new animal model (quail), to assess the synergistic efficacy of honey and black seed against demyelination within brain. A total of 35 male quails were used, among 10 w...

Zuhair Dardona1,2, Adnan Al-Hindi3, Mohamed Hafidi1, Ali Boumezzough1 and Samia Boussaa1,4*

...his purpose, 60 red meat samples were collected from butcheries in Gaza city, during the period from January to March 2021. These samples were divided evenly between beef and mutton. This study found that beef is devoid of T. gondii, whether tested using ELISA or PCR. On the contrary, both approaches detected T. gondii in mutton; however, the percentage ...
Qun Lu1,4, Yang Zhou1, Hangyu Lin1,4, Zhong tang He1, Feifan Ma1, Yonghua Zheng1,2, Hongyu Tang1,2 and Tao He1,2,3*
...r, the micro-features of sagittal otoliths of Schizothorax grahami and Spinibarbus sinensis were analyzed in the upper reaches of the Yangtze River. The results of age identification showed that Sc. grahami and Sp. sinensis did not reach the age of sexual maturity. The linear discriminant analysis of otolith shape-index analysis can effectively distinguish the otolith shape of the two kinds of fish. The highest content of five chemical elements in otolith is C...

Zhenkun Zhao1,2, Ziniu Alimo2, Xinyue Zhao2, Haifen Qin1, Buddhi Dayananda3, Lichun Jiang1,2* and Wei Chen4*

...lecular markers and taxa samples and use both effectively to reconstruct a better resolution for disputed species.

...

Fika Yuliza Purba1,4*, Andi Magfira Satya Apada1, Andi Ariyandy2, Irwan Ismail1,4, Subaedy Yusuf3,4

...ion. A total of 24 blood samples were collected from Bali cattle, then grouped into 4, based on the estimated age of lesion inflicted on the animals: Group A (no lesions), Group B (early stage), Group C (advanced stage), and Group D (recovery stage). The results showed that the white blood cell and granulocyte count in Group B was significantly higher than in Group A, while the lymphocyte and mid-cell count in Group B were higher compared to the other groups. ...

Shakil Ahmed1, Mahin Das2, Md. Rayhan Sojib3*, Shishir Kanti Talukder4, Sadia Sultana5, Prantika Datta1, Shofiqul Islam1 and Gazi Md. Mohsin2

...ditions, particularly in saline conditions. However, choosing the right variety is a challenge. In 2019, a field trial was conducted at Nobogram Farm in Noakhali, Bangladesh to evaluate the performance of local and exotic varieties of Yardlong bean under saline conditions. The experiment involved seven traits, including germination percentage, days to first flowering, fruit weight, fruits per plant, fruit length, seeds per p...

Muhammad Usman1, Majid S. Hashmi1, Ayaz Ahmad1*, Fawad Ahmad2 and Zahid Alam1

...pared to the sugar-based sample and other stevia-containing samples. During the organoleptic assessment, OD2 consistently received higher scores from the panel of judges throughout the storage period. Statistical analysis confirmed that this particular formulation had a substantial impact on both the physicochemical and sensory characteristics of the drink. For commercialization of the product large scale evaluation should b...

Iqra Munir*, Farrah Iftikhar, Hira Fatima, Sunbal Khalil Chaudhari and Roha Ramash

...nducted for all gathered samples. In this research study, documentation was carried out for 50 plant species distributed across 27 families. These species arranged with scientific names, common names, family names, plant part used and ethnopharmacological applications. There were 20- herbs, 13- shrubs and 18 tree species documented on the basis of their importance and medicinal uses. Frequently utilized plant components included were fruits, leaves, roots, bar...

Ping Sheng Ye1, Lin Li2, Yu Long Wu3 and Yuan Shu Zhang1*

...ing the experiment, milk samples were collected to assay the content of milk compositions; plasma samples were collected to measure the content of IGF-1 and GH. The liver and mammary gland samples were collected to observe the expression of IGF-1R and GH. The results showed that compared with the control group, the content of plasma GH and IGF-1 were dec...

Philip O. Akporhuarho1, Ufuoma Godstime Sorhue1*, Adimabua Mike Moemeka2, Onyinye Stella Onwumere-Idolor3, Jonathan Ujomu1, Emmanuel Abadah1, Jennfer Aaron1

...bjected to Aframomum chrysanthum seed meal (ACSM) as a replacement for synthetic antibiotic in broiler production. A total of one hundred and forty-four day-old Aboracre strains of broilers were randomly allotted into four dietary treatments. Treatments one (control) had no ACSM in the diet but was given routine antibiotics medication for broilers. Treatments two (T2), three (T3), and four (T4) had ACSM at 1%, 1.5%, and 2% inclusion levels. The experimental di...

Elie Brigitte Mawussi1*, Arnaud Koffi Assah2, Essozimna Abalo Kulo1

Dio Fico Felsidan Diatmono1, Seraphina Kumala1, Pradita Iustitia Sitaresmi2, Stefani Winda Paramita1, Megawati Andi1, Yustina Yuni Suranindyah3, Diah Tri Widayati1*

...3, and multiparous). The same management was given throughout this research and the feed intake was observed daily. The blood was taken during the follicular and luteal phase which was validated from data of vaginal smear. The obtained serum from whole blood was immediately for total protein, cholesterol, glucose, and blood urea nitrogen (BUN) levels assay. The spectrophotometry method used for biochemical data using specifi...

Hayder S. Rwayyih*, Tahani S.S. Al-Azawi

...ma changes in postmenopausal and it becomes multilayered due to migration of smooth muscle cells from the media to intima). In conclusions, the result of the current study could prove that Alpha lipoic acid has a protection on cardiovascular system in menopause estrogen deficiency and ovariectomized female rabbits. Alpha lipoic acid could fight the deleterious effect of menopause estrogen deficiency. 
 
Keywords | Ovariectomy,...

Bushra AL-Khaqani, Amira Mohammed*  

...ducing Evans blue extravasation in the lung (P<0.05) and liver (P>0.05). Additionally, in the BALF, RES therapy reduced (P<0.05) inflammatory cytokines including interleukin-17 (IL-17) and interferon-gamma (IFN-γ). RES increases (P<0.05) the expression of Il10 gene in the spleen, which is associated with T-regulatory and anti-inflammatory processes in the immune system. Our findings showed that RES may reduce asthma by activating anti-inflamm...

Abdelghafour El-Hamzaoui*, Mouloud Lamtai, Laila Ibouzine‑Dine, Sofia Azirar, Mohamed Yassine El-Brouzi, Ayoub Rezqaoui, Aboubaker El-Hessni, Abdelhalem Mesfioui

... widespread use of glyphosate-based herbicides (GBH) in agricultural practices raises major concerns regarding potential human toxicity. Mounting evidence from prior studies has delineated a direct link between GBH exposure and the development of neurodegenerative disorders. These compounds are also suspected of being involved in the induction of affective disorders. The present study was undertaken to examine the dose-dependent impact of GBH exposure over 8-w...

Saadatin Nurul Jannah1, Edhy Sudjarwo1, Osfar Sjofjan2*

...atment used was T0(-): basal diet, T0(+): basal diet + antibiotic (zinc bacitracin 0.10%), T1: basal diet + 0.50 % garlic peel, T2: basal diet + 0.50 % shallot peel, T3: basal diet + 0.50 % onion peel, T4: basal diet + 0.25 % garlic peel and 0.25% shallot peel, T5: ba

Ahmed Kareem Kadhim Al-Wasmee1, Waddah Salam Hassone2, Hawraa Talib Al-Janabi3, Hamed AH Al-Jabory1

...inety jugular vein blood samples were collected. The calves ranged from <1 M to ≥4 M and both sexes were represented. Two ml of blood were placed in sterile, EDTA-treated tubes, and were sent to the parasitology lab in an ice pack. The blood was put in a deep freezer set at -20oC for DNA extraction. This genomic DNA purification was used following the instructions provided in the kit to extract the parasite DNA. The results revealed that the rates of inf...

Noor-ul-Ain1, Zulfiqar Ali1*, Saba Naz1, Kiran Saleem1,2, Syeda Rida Hasan1, Sana Arif1, Rida Ahmad1,3, Nida Naeem1, Zarmina Zainab1 and Aliza Batool1,3

...ta was collected by scan sampling method of 70 h, documenting the cage area and behavioural ethogram. The observed behaviours were resting, eating, drinking, locomotion, aggression, urination, defecation, fighting, pacing, playing, foaming, cage exploration, and being out of view. Two different enrichments were installed for two pairs. The first pair was provided with a feeding enrichment consisting of a large wood log applied with small wooden branches on it,...
Asad Ali1*, Khalid Javed1, Imran Zahoor1, Khalid Mahmood Anjum2, Numan Sharif1, Imtiaz Ahmad3 and Aftab Hussain Aftab3

Muhammad Asif Iftikhar1, Talat Naseer Pasha2, Saima Inayat1, Khalid Javed3 and Rahman Ullah4*

...evaluation of all yogurt samples was carried out on a 9-point hedonic scale. All the tests were carried out on day 0, 7, 14 and 21. The collected data was investigated through ANOVA technique under complete randomized design (CRD) using SAS 9.1 software. The results showed that yoghurt made from Nili Ravi buffalo milk (To) showed a significant (p≥ 0.5) overall acceptability as compared ...
Li Tian1*, Chun Wang2, Hailiang Li2, Haitian Sun2 and Chang Wang3
...PCA-SVM (81.83%). At the same time, through the comparison of CBPSO algorithm, BPSO algorithm and PSO algorithm, it is found that CBPSO algorithm has great adaptability, the maximum fitness is 0.99, and the average fitness is 0.97. Therefore, CBPSO algorithm has good effect on global optimization, and its convergence speed is faster, and its execution task and efficiency are higher. In addition, among the five particle swarm optimization algorithms, the CBPSO ...

Noura K. Al-Suwailem1, Nancy N. Kamel2, Ahmed O. Abbas1,3*, Farid S. Nassar1,3 , Dalia A.A. Elsayed4, Gouda F. Gouda1,5, Hosam M. Safaa6

...ntrol group received a basal diet, while the other two groups experienced cyclic thermal stress from day 22 to 42 and were fed the basal diet provided with either 0 or 2 g MLM/kg diet. On day 42, we reported carcass characteristics and evaluated meat yield and quality. Blood samples (n=10) were used for hematological assessment, as well as plasma was employed to measure blood metabolites a...
Luis Fernando Díaz-Toral1, Carlos Eddy Alvarado-Zamora1, Ernesto Gonzalo Cañarte-Bermúdez2*, José Bernardo Navarrete-Cedeño2, Gilmar Jesús Cañarte-Cañarte1 and Fernando David Sánchez-Mora1* 
Julio Adolfo Corzo-Bacallao1*, Carlos Alfredo Salas-Macías1, Osvaldo Fonseca-Rodríguez2,3, Felipe R. Garcés-Fiallos1, Erika Isabel Alcívar-Muñoz1 and Henry Fabricio Baque-Loor1 
 
...d out in a context of peasant family agriculture, with an agroforestry system with coffee trees of the Sarchimor variety planted at 1.5 x 1.5 m, interspersed with tree species typical of the dry forest. The system involves manual weed control, without fertilization, irrigation, phytosanitary control, or shade regulation. In this scenario, and during an experimental period of 90 days (03/08/2022 - 26/10/2022), phenological va...

Minh Thi Le Bui1, Thanh Minh Le2, Thuan Khanh Nguyen1*

...er supplemented in the basal diet on the immune response against the Newcastle Disease (ND) virus and the growth performance of the local chickens. One-day-old chicks (n=400) were allocated to a completely randomized design with four replicates of five treatments. Chickens in treatment T1 were not supplemented with any powder in the basal diet. Chickens in treatment T2 were supplemented with chlortetracycline with 10 mg/kg f...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...accine strains of the causative agent of emphysematous carbuncle used for active immunisation of animals in Kazakhstan and field experiments with biological material from sick and deceased animals, results were obtained that indicate that the developed test system can be used to diagnose animal diseases. Testing the system on vaccine strains showed a highly specific immunological reaction of the test drug to the cau

Thuong Thi Nguyen1*, Nhu Quynh Ho1, Thuan Khanh Nguyen2, Tuan Phong Vu Anh Vo3, Hai Thanh Duong4

...amination, in which abomasal displacement was 100% alert effective, ketosis at 73.53% alerts, and mastitis at 63.83% alerts. However, the rates of mastitis, ketosis, and abomasal displacement were 24%, 13.6%, and 10%, respectively. On the alert day, rumination time and milk yield of diseased cows were lower than those of non-diseased cows (P<0.05). Rumination time and milk yield decreased markedly on the alert day compare...
Yanis Cruz-Quintana1*, Ana María Santana-Piñeros1, Byron Manuel Reyes-Mero1, Leonela Griselda Muñoz-Chumo1 and Lenin Cáceres-Farías1,2 

Muhmmad Asif1, Khunsa Khakwani1, Muhammad Hasnain1*, Farrukh Ilahi1, Muhammad Hussnain Babar1, Shahid Munir Chuhan1, Jehanzeb Farooq1, Hafiz Ghazanfar Abbas1, Iqra Parveen1, Ghulam Sarwar2, Saeed Ahmad2 and Hammad Hussnain2

...limatic conditions of Faisalabad.

...

Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1

...kaloids, glycosides, and saponins) in the bark of V. negundo (L.) roots and stem, as well as their potential antioxidant and antibacterial properties. The crude was obtained in 95 % methanolic which was further fractionated into n-hexane (HR, HS), Chloroform (CR, CS), ethyl acetate (EtR, EtS) and butanol (BR, BS) soluble fractions by solvent-solvent extraction. In phytochemical tests of roots fractions; HR showed the presence of terpenoids, while

Muhammad Ayyub, Mitha Khan*, Syed Abdul Malik, Sakhawat Ali, Syed Shamsullah, Mujeeb ur Rehman, Ikhlaq Ahmed, Ameer Uddin, Habibullah Kakar, Mohammad Zahid, Khalil Ahmed, Mana Khan, Mukhtiar Ahmed, Muhammad Rafiq Khetran, Zia ul Haq and Muhammad Azam

...ght, Flag leaf area, thousand grain weight, Grain yield plant-1 and Tones per hectare as showed highly significant positive general combining ability, respectively. While among TD1 × Benazir and Umeed × Raskoo were observed as good specific combiner crosses in all parameters. Further, it is recommended that these genotypes be further included in breeding program to get more cultivar and hybrids combiner to enhance yield through yield contributing t...

Muhammad Ali Raza1*, Aneela Zameer Durrani2, Bilques Bano3, Muhammad Muddassir Ali4, Nazia Rubab5, Syed Tasadak Mehdi6, Muhammad Wasim Iqbal7, Kumay Hassan Akhtar8, Aqeel Raza9, Ujala Fatima Shan10, Muhammad Aftab11

...lopment of livestock. 90 samples of each weigh about 50 grams have been collected from Lahore and brought to University of Veterinary and Animal Sciences, Lahore in a Laboratory and applied HPLC technique on samples and found residues of Penicillin and oxytetracycline in different percentages (11% and 9%, respectively) and percentage of both combine Penicillin and oxytetracycline is 3%. In a yoghurt ...

Huma Naz1*, Sajid Abdullah2, Tanveer Ahmed3*, Khalid Abbas2, Syed Qaswar Ali Shah1, Muhammad Rizwan1, Fareeha Latif4, Adnan Ahmad Qazi1, Muhammad Adeel Hassan5

...Comet and Micronucleus Assay. Twenty fingerlings of L. rohita was kept in LC50 concentration of CPF+END, (1.95±0.02 μgL-1 for 96 h) for 5 days after acclimatization. The blood sample of 5 fish was collected after 24, 48, 72 and 96 h to see the DNA damage and nuclear anomalies. Results of the current study revealed that damaged nuclei (DN%) and genetic damage index (GDI) in RBCs of L. rohita exposed to insecticides ...

Asmit Subba1*, Jash Hang Limbu2 and Laxman Khanal1*

... facilitated their dispersal. Further studies are warranted to understand the detailed taxonomy and distribution pattern of H. nani in Nepal. 
 
Novelty Statement | This study reported the occurrence of Heteropeustes nani from Nepal using field surveys from water bodies of eastern lowland of the country. Further, it characterized the habitats of the species and proposed...

Bilal Atta1*, Arshed Makhdoom Sabir1, Muhammad Dildar Gogi2, Muhammad Asif Farooq3, Muhammad Ijaz1, Tahir Hussain Awan1, Muhammad Ahsin Ayub4, Muhammad Usman Saleem1, Amara Nasiba1

... Scirpophaga innotata, Sesamia inferens, Scirpophaga incertulas, Cnaphalocrocis medinalis, Sogatella furcifera and Nilaparvata lugens. Population data spanning four years (2019-2022) were collected through the use of light traps revealed distinct peak populations for each species at different times. Scirpophaga innotata exhibited a consistent peak population in September, with a significant increase from 2019 to 2020, followed by a decline from 2020 to 2021. H...

Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3

...o ensure the quality and safety of these vaccines, quality control assessment was carried out. The efficacy evaluation of the vaccines was performed on 24 locally bred rabbits, divided into 4 groups. Each group received a different type of vaccine, with booster doses at 15-day intervals following the priming. Serum samples were collected biweekly over a 60-day period, and the antibody titers were measured using IHA test. Geo...

Jinzhao Li1, Yawen Zhang2, Binghan Jia1, Yuqiong Zhao1, Huijuan Luo1, Xiaojie Ren1, Yuan Li3, Xiaoyan Bai1, Jing Ye3 and Junping Li1*

...rs (MORs) in the colonic samples. The gene sequencing approach was used to detect alternatively spliced tau isoforms in the distal colonic muscle layer. Sequencing analysis revealed that 3 isoforms of tau, namely 2N4R, 1N4R and 0N4R were expressed in the distal colonic myocardium tissue. OIC rats had small, dry and hard feces, and intestinal propulsion velocity was significantly slower, especially in the distal colon. P-Ser396-Tau, P-Ser404-Tau, PKC, p-p38, p3...

Shangmei Peng1 and Changhe Yu2*

...en the two groups at the same time point (P>0.05). The incidence of eGFR 5 patients in the two groups was higher than that in other stages, and the difference was statistically significant (P<0.05). Excessive serum creatinine before CT, diabetes, and female gender are independent risk factors for secondary AKI (OR>1, P<0.05). The incidence of PC-AKI after enhanced CT examination is low, and it has nothing to do with intravenous contrast agent. It i...
Danish Riaz1,2, Syed Makhdoom Hussain2*, Majid Hussain3, Muhammad Zubair-ul-Hassan Arsalan4 and Eman Naeem2
...d 3 g/kg) added to the basal diet. Fish growth performance, nutritional bioavailability, and hematological indices all improved significantly (p<0.05) after being supplemented with probiotics. Fish fed a diet supplemented with probiotics at a rate of 2.5 g/kg had substantial improvement in body weight (20.29 g), body wt.% (272.78 %), feed conversion ratio (1.24) and specific growth rate (1.46). At a supplementation dosage...

Moazama Batool1*, Syeda Ansa Fatima1, Saima Naz2*, Qurat Ul Ain1, Sheeza Bano1, Ghulam Abbas3 and Ahmad Manan Mustafa Chatha4

...opercular movement, somersaulting activity, convulsions rate, air gulfing was significantly (p<0.05) increased by increasing the bifenthrin concentration. Fin movements and swimming rate was also increased with increase in bifenthrin concentration but at later stage fish became motionless and sluggish. Body color was changed from grey to pale and gills from bright to light red color as bifenthrin concentration increased. Blood parameters such as red blood c...

Khurram Nawaz Saddozai1, Muhammad Nasrullah2, Jahangir Khan3*, Syed Attaullah Shah1, Raheel Saqib4, Naheed Zahra5 and Mansoor Rasheed6

... to face interview and a sample of 120 tobacco growers was selected. These growers were randomly selected from three villages namely Takar Kaly, Garo Shah, and Pasand Kaly of tehsil Takhtbai, district Mardan of Khyber Pakhtunkhwa. Empirical findings reveal that labour, chemical, irrigation, seed, poultry manure, and tractor have positive and statistically significant effects on tobacco production. Results of the study also c...

Akbar Khan Khajjak1,4*, Adnan Nazir2, Tehmina Mangan1 and Ali Raza1

... evident that efficient usage of resources could add Rs.594.77 ($4.10) million of revenue each year to the economy. The research is based on and derived from wheat production, which is only for a single crop among various other crops. These findings would be quite useful in developing a precise policy for major food crops in Pakistan. These facts would support policy developers to divide resources with equal allocation between agricultural and services sectors...

Hilarius Yosef Sikone1*, Gomera Bouk2, Edelnia Kristina Bere2, Yohana Kamlasi2, Elisabeth Yulia Nugraha1

...ency, province of East Nusa Tenggara, in the sub-districts of West Miomaffo, Central Miomaffo, Insana, North Insana, Biboki Moenle’u, and Biboki Anle’u between April 2023 to October 2023. 285 Balinese cattle, including 82 calves, 105 weaned beef cattle, and 98 heifers. This research used mixed methods to obtain qualitative and quantitative data, a survey was conducted by 180 br...

Hanita Fadhilla1, Shafia Khairani1,2*, Ahmad Fitrah3, Wendi Prameswari4, Nur Purba Priambada4, Indri Saptorini4, Imam Arifin4

...s were documented by Yayasan Inisiasi Alam Rehabilitasi Indonesia (YIARI), accompanied with radiology results and anatomical gross pathology examination. Medical records were evaluated based on lesions, clinical findings, radiological findings, surgical or conservative management (treatment), and survival rates. Results showed that twelve slow lorises did not show clinical conditions, while others experienced ballistic wounds and swelling of the lymphoglandula...

Apurbo Kumar Mondal1, Md. Rabiul Auwul2, Md. Momotaj Hossen3, Md. Sodrul Islam1*, Narayan Paudyal4, Md. Shahidul Islam1 and Kazi Khalid Ibne Khalil1

...sex, study duration, and sample size for the assessment of the potential risk factors. The prevalence rate was found highest in sheep at 42.91%, female individuals at 32.32%, 500 or below sample size at 43.63%, in the period of 2000–2010 at 42.05% and aged animals at 41.23% non-significantly. This is the meta-analysis of PPR worldwide that offers a comprehensive picture of the prevalence of PPR in small ruminants with ...

Ali Hazrat1*, Qasir Ali2, Mohammad Nisar1, Khan Sher2, Tour Jan1 and Abid Ullah1

...ed by epiphyllous (30%), saxicolous (20%), and terrestrial (10%). Further
studies are suggested to further confirm our findings.
...

Rahamdad Khan1, Abdul Basit, Syed Majid Rasheed* and Syed Salim Shah

...acha Khan University Charsadda Pakistan during summer,
2018. The experiment was carried out in CRD design with factorial arrangements repeated
thrice. Five maize varieties viz., ‘Azam’, ‘Babar’,s Pahari’, ‘Iqbal’, and ‘Jalal’ comprised
factor A while, Factor B consisted of water extracts from root, stem and leaf of invasive
weeds; Parthenium hysterophorus and ...

Gulwaiz Akhter1* and Tabreiz Ahmad Khan

...ca, O. cernua and O. ramosa were found to
be associated with brinjal (Solanum melongena) in Banda district, India. However, the
concomitant infestation of O. aegyptiaca with O. cernua and/ or O. ramosa in brinjal crop
was not noticed. Moreover, some of brinjal fields showed the infestation of O. aegyptiaca
with either O. cernua or O. ramosa. ...
Mona Adel El-Wakeel1*, Salah El-Din Abd El-Ghany Ahmed, Sanaa Abd El Rahman Mohamed
and Nadia Khalil Messiha
...and natural products are safe non-chemical modern techniques that are
applied as alternative to synthetic herbicides for controlling weeds. So, two pot experiments
were conducted to evaluate the allelopathic effect of Psidium guajava leaf powder (PLP) and
Acetic acid 5% as a natural products on the growth and yield of Capsicum annuum plants
and both associated weeds: Phalaris minor (grassy weed) and Malva parvifl...
Iqra Munir1, Abdul Ghaffar2, Asad Aslam2, Muhammad Khuram Shahzad1 and
Muhammad Jafir3
...ersity of Agriculture Faisalabad, Pakistan. Treatments consisted of different
combinations of weeds and control group. Total 228 samples were collected and from these
samples 14 different taxons of soil arthropods were reported and from these taxa, mites
showed the highest diversity index. Maximum diversity of soil arthropods was recorded
(H&...

Nimra Javaid1, Mukhtar Hussain Shah1, Iqra Haider Khan2, *Arshad Javaid2 and Syed Muhammad Waleed1

... used in laboratory bioassays. ANOVA revealed the significant effect of plant part bioassay and extract concentration on germination as well as various root and shoot growth parameters. Leaf extract was found as the most herbicidal where a 10% extract completely inhibited the germination and growth of parthenium. Root and inflorescence extracts were also highly inhibitory in suppressing the germination up to 89% and 95%, sho...

Ali Hazrat1

...type of species of the Rosaceae family in the target area. In total, 30 species were collected belonging to 15 genera from different localities of the selected area, in which the highest number of seven plant species belonged to genus Prunus, five to genus Rosa, three to genus Potentilla, two to each genus Cotoneaster, Rubus and Pyrus and one to each genus Crataegus, Eriobotrya, Fragaria, Malus, Sibbaldia, Sorbus, Sorbaria a...

Mona Adel El-Wakeel1 and Ibrahim Mohamed El-Metwally1

...>the soil surface at the same rates but directly at the same time with sowing of common
bean seeds. Additionally, four untreated control treatments were applied for comparison.
The recorded results revealed the inhibitory allelopathic effects of orange peels powder
on both weeds with direct relationship between the orange peels rate and it's inhibitory
effects. However, the pre...

Farrukh Hussain1, Sajid Aziz1, Gul Hassan2, Khalid Aziz1 and Sapna Raisham1

...ork on weeds of Cannabis sativaL. fields. Although, the cultivation of C. sativa is legally banned in Pakistan vide the Control of Narcotics Substance Act of 1997, yet it is a regular cash crop in Tirah-Maidan Valley. Cannabis sativa fields were analyzed in three localities: Kalona, Zangai and Kawarli during August 2019. Ten fields were analyzed using 10, 1m2quadrats in duplicate for the i...

Felix O. Takim*1, Gideon Z. Nayan2, Oluwafemi O. Osatuyi1and Israel O. Olayiwola1

...ht-prone southern Guinea savanna of Nigeria. The experiment was laid as a randomized complete block design with a split-plot arrangement and 3 replications in 2018 and 2019 growing seasons. The main plots were planting time (May, June and July) while the sub-plots consisted of 3 maize-sweet potato intercropping populations(maize at 53,333 plants/ha + 33,333 plants/ha of sweet potato, maize at 53,333 plants/ha + 66,666 plants/ha of sweet potato and maize at 53,...
Muhammad Ehsan Safdar1, Muhammad Ather Nadeem1, Abdul Rehman1, Amjed Ali1,
Nasir Iqbal2, Qaisar Mumtaz1, Amir Javed1
Tabassum yaseen1*, Muzamil shah1, Khushnood Ur Rehman2 Ali Mujtaba Shah3, Gul
Nawaz1,Rani Gul4
Muhammad Ather Nadeem1, Bilal Ahmad Khan 1, Sadia Afzal2, Muhammad Azim
Khan3, Tasawer Abbas4, Muhammad Mansoor Javaid1, Muhammad Mohsin Amin1,
Naila Farooq5 Amir Aziz5
...Carthamus tinctorius L. (safflower) on summer weed Oryza punctata
L. (red rice) seed emergence and initial seedling growth, a study was planned. In this study,
seeds of Oryza punctata were incubated in seven concentrations (0, 0.25, 0.50, 1, 2, 4, and
8%) of different parts i.e. leaves, stem and fruit of C. tinctorius. All the tested
concentrations of various plant parts of C. tinctorius significantly inhibited t...
Luqman1, Zahid Hussain1, Mohammad Ilyas2, Azmat Ali Awan3, Javed Rahman 2,
Mazhar Ali2 and Tamana Bakht4
... newspaper as mulch, and saw-dust as mulch), a herbicide i.e. Stomp
330 EC (pendimethalin) applied as pre-emergence. For comparison of the treatments with
weed free and weedy conditions, a hand weeding and a weedy check (control) treatment
were also kept. Data were collected on weeds fresh biomass, number of plants ha-1 and fruit
weight. The results showed significant effect for all the parameters during both yea...
Fawad Khan1, Zahir Muhammad1, Khushdil Khan2 , Shabir Ahmad2, Muhammad Jamil Khan3, Tamana Bakht4 And Asif Kamal2
...on microscope (SEM), the samples were prepared via
acetalization process. We done the fieldwork for the collection of weed plants and also
performed the experimental work i.e. SEM and LM for the pollen morphology. Most of the
species recorded with pollen of tricolporate and echinate. Species belonging to Asteraceae
were considered as most abundant and allergenic as compared to others. The maximum
pola...

Khalid Ali1, Asif Tanveer1, Naila Farooq2, Tasawer Abbas3*, Ghulam Sarwar2, Muhammad Ather Nadeem4, Ishtiaq Hassan3, Muhammad Mansoor Javaid4, Anees-ul-Hussnain Shah5, Bilal Ahmad Khan4 , Ali Raza6

...justify;">Increased soil salinity under changing climate has complicated weed management. Rhynchosia capitata (Clustered-flower Snoutbean) has become problematic weed in summer crops, such as cotton, soybean, pearl millet and mungbean worldwide. Current study was conducted to evaluate the impact of four types of salts stresses (NaCl, Na2SO4, CaCl2 and NaHCO3) at six different levels (0, 50, 100, 150, 200 and 250 mM) on R. ca...

Kiran Rani1*, R. Aliya1, Barkat Ali Solangi2, M. Kashif Pervez2, Nasrullah Akhtar3 and Farman Ahmed4*

...tested organisms. Fabric samples showed greater antimicrobial efficiency for Candida albicans and poorer than for Escherichia coli.

...

Syeda Fakehha Naqvi1, Iqra Haider Khan1 and Arshad Javaid1

... piperine (4.75%), nonacosane (4.69%), monoplalmitin (4.21%), γ-sitosterol (3.91%), methyl linoleate (3.88%), neocurdione (3.86%) and ethanonone (3.25%). The compounds such as stigmasterol (2.92%), 1,4-benzenedicarboxylic acid (2.78%), tetracosanoic acid (1.19%), stearic acid (1.36%), tridecanoic acid (1.35%), tridecanal (1.30%), phytol (1.29%), docosanoic acid, methyl ester (1.23%),...

Iqra Haider Khan1 and *Arshad Javaid1

...lign: justify;">Cannabis sativa L. is a medicinally important plant, generally grows as a weed in waste lands in Punjab, Pakistan. In the present study, antifungal effect of leaf extract of this weed was assessed against Aspergillus flavipes. Methanolic leaf extract of the weed was partitioned into five fractions using organic solvents of variable polarities. A range of concentrations (1.562 to 200 mg mL-1) of each fraction was used in laboratory bioas

Haroon Khan1*, M. Mubassir Khan1, Bakhitar Gul1, M. Fawad1 and Imtiaz Khan1

...), followed by Digiteria sanguinalis (14.87), Cyperus rotundus (12.96) and Euphorbia prostrata (5.12). Parthenium hysterophorus L. an invasive alien weed was recorded in almost all the sites with a density of (2.6 m-2) in the non-field areas particularly, followed by (0.85 m-2) in field crop and (0.8 m-2) in orchards and with a mean density of (1.42 m-2) and a relative density of (1.52%) across all locations. Similarly, another invasive weed Broussonetia papyr...

Shazia Shafique1, Sobiya Shafique1 and Sonia Sahar1

...igatory to reduce the causal agent lower than commercial thresh hold level. Biological control provides safe fungal management program. Presently, the research was under taken to ascertain the antitoxic effect of Thymus serpyllum against eggplant leaf spot pathogens i.e., Exserohilum rostratum, Cladosporium cladosporioides and Curvularia clavata. For this, 10 concentrations of methanolic extract of plant (0.5% to 5%) were em...

Sanaullah1*

...A three stage stratified sampling technique was adopted to collect data from the selected respondents through a well-designed interview schedule. Statistical Package for Social Sciences (SPSS v 20) was used to analyze the primary cross-sectional data and the obtained findings were depicted in tables and figures. Descriptive statistics revealed that majority of the respondents i.e. 31.3% were of middle age with 63.9% illiteracy rate and 60.8% reported living in...

Salman Ali1*, Muhammad Arif1, Mehran Ali1, Muhammad Afzaal2, Gohar Saeed32, Muhammad Bilal4, Fazal Munsif1 and Sajjad Zaheer1

...a cornerstone practice. Usage of high doses of herbicides for killing weeds has created several ecological and environmental problems which cannot be overlooked. Therefore, other weed control practices and techniques should be adopted to minimize weed-crop competition and increase the growth and productivity of crop, instead of chemicals. An experiment was established at Agronomy Research Farm, the University of Agriculture Peshawar to evaluate the effects of ...
Farrukh Avais1, Anser Ali1, Hamza Javed2, Muhammad Saleem3, Tehreem Tahir4,
Muhammad Rafiq5,*
...d roots of Garlic Allium sativum (AS) and Peppermint Mentha
piperita (MP), which were further processed for 1:1 fractional distillation to prepare
methanolic n-hexane (MeOH_n-Hx), methanolic ethyl acetate (MeOH_EA) and
methanolic chloroform (MeOH_CHCl3) extracts, aiming to evaluate tyrosinase and antioxidant
activities in-vitro. Our results confirmed that all MeOH-crude AS and MP extracts
showed signi...
Naeema Khatoon Khaskheli1 , Muzafar Hussain Sirohi11, 2,*, Ameer Ahmed
Mirbahar1, Abdul Razak Mahar1, Mumtaz Ali Saand1, Mirza Hussain3
...nd herbarium
samples were preserved in Herbarium, Shah Abdul Latif University, Khairpur, Sindh
Pakistan, for future reference. The species community composition, habit, and life span
were determined. The study confirmed 35 weed species belonging to 14 plant families. The
weed community was dominated by the plant families Poaceae (8 spp.), Amaranthaceae (7
spp.), and Aizoaceae (3 spp.). The most freque...
Imtiaz Khan,1 Muhammad Kabir, Muhammad Ishfaq Khan, Haroon Khan, Saima
Hashim and Muhammad Azim Khan
...ds, line sowing provided satisfactory
results. As general understandings various weeds were managed significantly through tank
mixed herbicide applications with a ratio of 60-72% for broad leaf and grassy weeds
respectively. Consequently the instant results provided 54% increased yield compared to
the untreated treatments. Hence it is concluded that, line sowing in combination with tank
mixed herbicid...

*Arshad Javaid1, Syeda Fakehha Naqvi1 and Iqra Haider Khan1

... phthalate (4.99%); tricosanoic acid (2.74%); dioctyl phthalate
(4.99%), hexanal (3.05%) and ergostane (1.29%). Literature survey showed that 10 of the
identified compounds exhibited various biological activities including antifungal,
antibacterial, antioxidant, anticancer and antipsoriatic. However, this study concludes that
most of the compounds were antimicrobial in nature.
...

Iqra Haider Khan1, *Arshad Javaid1 and Nadeem Shad1

...xtract of hemp (Cannabis sativa
L.) leaves against A. versicolor. Dried leaves of hemp were soaked in methanol for two
weeks. The material was filtered and the methanol was evaporated on a rotary evaporator.
Thereafter, water was added to the residues and the aqueous mixture was successively
partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. Different concentrations
of each fraction w...

Shabnam Javed1, Amna Shoaib2 and Zaid Mehmood1

...div>...

Shabnam Javed1 and Amna Shoaib2

...r Berre [Sorbaria tomentosa (Lindl.) Rehder] exhibits
medicinal value as a large woody shrub. The present study examined the cytotoxic activities
of S. tomentosa using methanolic extracts and fractions (n-hexane, dichloromethane, ethyl
acetate and water) against three cancer cell lines (lung A-549, hepatocellular HepG2and
urinary bladder EI-138). Cytotoxic as
Muhammad Sikander Hayyat1*, Muhammad Ehsan Safdar1, Muhammad Mansoor
Javaid1
... rice (Oryza
sativa L.) that greatly reduces in yield. The laboratory studies were undertaken to confirm
allelopathic potential of its plant leachates and soil-decomposition plant residues towards
emergence and seedling growth of rice. In first experiment, aqueous extracts from various
plant parts of red sprangletop (stem, root, leaves, flower and entire plant) at their 5% (w/v)
concentration were app...

Gulshan Riaz1, Zaheer-ud-din Khan1, Muhammad Umer Farooq Awan1, Andleeb Anwar Sardar1, Muhammad Tayyab1, Sarah Maryam Malik and Sohaib Muhammad1

...ard and wheat of Tehsil Isa Khel, District Mianwali, Punjab. Forty one (41) weed species were collected from the study area belonging to twenty one (21) different families. Twenty four weed species found in chickpea, twenty five in mustard and twenty nine in wheat crop fields. Sixteen weed species were common in three crops. Family Poaceae and Astraceae had maximum weed species i.e. 7 and 6 species respectively followed by Euphorbiaceae, Fabaceae, Chenopodiace...

Khushdil Khan1, Mushtaq Ahmad1, Muhammad Zafar1, Khafsa Malik2, Shazia Sultana1, Shabir Ahmad1, Fawad Khan3, Asif kamal1, Kalim Ullah3

...a, Datura innoxia, Eruca sativa, Convolvulus arvensis, Anagallis arvensis, Galium aparine, and Anethum graveolens. Slides for light microscopic studies were prepared using materials of acetic acid, glycerin jelly and anthers of flowers. Pollen grain recorded ranged from monocolpate to hexacolporate and from psilate to echinate which were important systematic significance. Pollen size, shape, ratio of polar to equatorial diameter exine thickness, number of colp...

Muhammad Nasir Mazhar1, Muhammad Arif*, Naeem Akhtar1 and Muhammad Shafiq1 and Muhammad Yousaf1

...s i.e. Ujala 2016 and Faisalabad 2008 and seven weeds control approaches i.e. hand weeding, organic mulching, eucalyptus extract, neem extract, clodinafop, bromoxynil + MCPA and clodinafop + bromoxynil + MCPA including control. Results of the experiment showed that hand weedings, combined application of clodinafop + bromoxynil + MCPA and mulching significantly reduced the weeds density, fresh and dry weight of weeds. However, covering the soil surface with the...

Afrah Adil Hassan1*, Hawraa F. H. Alowaid1, Majdy Faisal Majeed2 

...kg/day. All animals were sacrificed 24 hours after the last oral dose administration upon completion of the 30-day trial. The liver was promptly removed for an assessment of the changes in malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase activity (MOP). Proinflammatory cytokines (TNF-α and IL-1β) were measured in the plasma samples, and an evaluation of the liver histological profile was conducted on ...

Mushtaq A.Alabodi1*, Imad Majeed Almeeni2 

... they were remained at basal level (under 20 pg/ml and 2ng/ml respectively) in both groups. In conclusion, the melatonin implant suppresses the estrus cycle for 4 months without any adverse effects and maintains the concentration of estrogen E2 and progesterone hormones at the basal level.  

...
Hasina Baloch1, Rahmatullah Rind2, Dildar Hussain Kalhoro1*, Shahid Hussain Abro1, Hubdar Ali Kolachi3, Saqib Kakar1, Muhammad Ibrahim3, Fayaz Ahned1, Hasnain Ali1 and Muhammad Mahesar1
...d 840 (210 animals) milk samples were collected from lactating buffaloes with signs of clinical and subclinical mastitis respectively. The California Mastitis Test was performed to confirm mastitis. The sub-clinical cases were categorized by severity of infection with moderate severe, severe, more severe and most severe. All the 646 positive samples analysed, 41.95% were found with flacks, 7.73% with watery consistency, 15.0...

Yuping Liu, Sige Wang and Tianyan Yang*

.... The analysis of codon usage showed that NNA-type codons were used most frequently, which was consistent with the A bias at the third codon positions in PCGs. Three base mismatches were detected in the secondary structures of tRNAs, namely A-C, U-U and A-A, which mainly occurred in the amino acid receptor arm and TψC arm. The CR contained three different domains: extended termination-associated sequences, central conserved region (CSB-F, CSB-E and CSB-D) ...
Wagner Antonio Gorozabel Muñoz1*, Mayra Jossenka Loor Solórzano1, Josselyn Gema Palacio Intriago1, Virginia Vanessa Andrade Andrade1 and Carlos Alfredo Cedeño-Palacios1,2 
 
...s, there has been a proposal to add value to a raw material that is underutilized by the Ecuadorian industry, such as squash. This was accomplished by evaluating its properties and conducting physicochemical, antioxidant, and beta-carotene analyses on the flours obtained from the pulp of three squash varieties: vermilion squash, pepo and macre. For the research project, the squash samples were subjected to a process of selec...

Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*

... by cell proliferation assay. The results showed that a target band of 583 bp was obtained by PCR in DNA vaccine PLGA microspheres group at 7th, 14th, 21st, 28th, 35th day post-injection. No 3-1E gene target band was found in the control group and the naked plasmid DNA vaccine group. It indicated that plasmid pcDNA3-1E existed in tissues of immunized chickens with DNA microsphere vaccine, and the plasmid pcDNA3-1E released from microspheres sustained at least ...

Haoneng Guo1,2, Shuangshuang Xiao3, Wenfang Lou1,2, Rifat Ullah Khan4, Juanjuan Wu1,2, Bo Huang2, Sifa Dai2,3* and Guanhong Li1*

... broilers were fed the basal diet, and the broilers in Group II, III, IV were fed a basal diet supplemented with 1.0% and 1.5%, respectively 2.0% Gln. The broilers were kept at 25±2 ℃ for 14 days. Samples were taken and analyzed at 7 and 14 days of age. On the 7th day, compared with group Ⅰ, the serum level of LDH in group II, III and IV were significantly decreased. Serum triiodothyronine content in group II, III...

Md. Shahidul Islam*, Md. Mizanur Rahman, Md. Sodrul Islam, Kazi Khalid Ibne Khalil and Apurbo Kumar Mondal

...birds were reared in the same environmental conditions. As a part of growth performances evaluation, daily body weight gain and weight improvement, weekly average feed consumption, feed conversion ratio, final average body weight and carcass weight were recorded during the experimental period. As a part of hematological analysis at 5 weeks of age, blood parameters including total erythrocyte count (TEC), packed cell volume (PCV), haemoglobin (Hb), erythrocyte ...

Wafaa M. A. El-Nagdi1*, Ahmed E.A. Mahgoob2, Mahmoud M.A. Youssef1, Mona M.S. Zayed3, Entesar H. Taha2 and Nora R.A. Saleh1

...olate+ AMF, added at the same time with nematode (Ne) or added at 7 days before Ne inoculation, showed additive or synergistic interaction effects. The tested isolates increased significantly (p≤0.05) plant growth criteria according to the tested materials. The same isolates +AMF with Ne at the same time recorded less colonization percentage (35%), while 40% root colonization was achiev...

Aya A. Saber1, Mohamed Fawzy2*, MokhtarM. EL-Tarabili2, Eman K. El Sayed2

...uires the utilization of safe and effective vaccines capable of successfully preventing and controlling these disastrous diseases. The current study explored the effect of simultaneous vaccination of cattle with BEF and RVF vaccines on the calves’ humeral immune response. Four groups of BEF and RVF seronegative calves were used in this study; group 1 received inactivated BEF vaccine, group-2 received inactivated RVF va...

M. Daud AK1,2*, Mahdi Abrar2*, Teuku Reza Ferasyi3,4, Harapan Harapan5,6,7,8

... August 2023. The Aceh Besar district was notably recognized as a prominent area with a high incidence of FMD outbreak followed by Aceh Tamiang and Aceh Utara. The data suggested that the occurrences of FMD were sporadic and randomly dispersed, as indicated by a Z-Score of -0.927 according to Moran’s I analysis. This study highlights that geospatial analysis could be employed to make informed decisions on different strategies, such as district-based isol...

Nguyen Thuy Linh, Nguyen Hoang Qui*

...especially, improving bursa of Fabricius and thymus weight (P<0.05). The inclusion of 15% and even 30% HI in diets to replace FM did not record changes in hematological and hepatic enzyme indicators. It can be concluded that FM replacement by HIM could improve growth and health of broilers without detrimental effect.
 
Keywords | Blood parameter, Broiler, Growth, Hermetia illucens, Hepatic enzyme
...

Claude Mona Airin1*, Miyayu Soneta Sofyan2, Galy Hardyta3, Khrisdiana Putri4, Pudji Astuti1

... (JE), Yogyakarta. Fecal samples were collected in the morning, afternoon, and evening for seven days, during low and high numbers of visitors. The collected fecal samples were freeze-dried for 72 hours, and 80% methanol extraction followed by Enzyme Immuno Assay (EIA) analysis on cortisol, T3 and T4 levels carried out. Other data, such as the number of visitors, mileage, and number of hor...

Fitrimawati*, James Hellyward

... in West Sumatera. Data usages are secondary data and primary data. The secondary data obtained from Statistics Central Bureau of West Sumatera. The primary data obtained through interviews, filling out questionnaires and Focus Group Discussions with stakeholders and experts involved in livestock development, such as breeders, government, private sector, academics and relationship institutions. The obtaining of data was analyzed using Analytical Hierarchy Proc...

Siti Zubaidah1, Chusnul Hanim2, Bambang Ariyadi3, Aji Praba Baskara2*, Zuprizal2

... with and without shells samples were used to study the chemical composition of PKC, and PKC with and without enzymes were used to investigate cell-wall structure. The enzymes consisted of mannanase (182 g/tons), nonstarch polysaccharide degrading enzyme (200 g/tons), and protease (130 g/tons). The chemical composition included dry matter (DM), crude protein (CP), ash, ether extract (EE), nitrogen-free extract (NFE), crude f...

Mthi S. 1,2, Ikusika O.O.1*, Washaya S.3, Mpendulo C.T.1, Nowers C.B.2 

...red weekly, while faecal samples were collected every fortnight. Faecal egg count was performed using the McMaster and modified Stoll methods, while body weight was measured using an electronic scale. Treatments had a significant difference (P<0.05) in the weight gain of lambs. Improvement in the average daily weight gain performance of lambs was also observed at 10% DME inclusion levels, followed by T1 (31.96±7.787 kg), T2 (31.86±7.787 kg), a...

Journal of Animal Health and Production

October

Vol. 11, Iss. 3, Pages 234-329

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