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Polymorphism in the Exon 11 of Bovine Luteinizing Hormone Receptor Gene and its Association with Service per Conception in Pasundan Cows

Polymorphism in the Exon 11 of Bovine Luteinizing Hormone Receptor Gene and its Association with Service per Conception in Pasundan Cows

Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2

1Research Center for Biotechnology, Indonesian Institute of Sciences, Bogor-Jakarta Rd. Km. 46, Cibinong, Bogor, West Java, Indonesia 16911
2Department of Animal Sciences, Faculty of Animal Science, Bogor Agricultural University, Agatis Rd., Dramaga Campus, Bogor, West Java, Indonesia 16680

*      Corresponding author: syahruddinsaid01@gmail.com

ABSTRACT

Bovine luteinizing hormone receptor (bLHR) gene in mammals is important for several reproduction function such as steroidogenesis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/HhaI gene with T allele as the dominant allele (0.61). Observed (Ho) and expected (He) heterozygosity had similar values of 0.48. The polymorphic informative content (PIC) was 0.37 and was of moderate category (0.25<PIC<0.50). The Chi-square value (χ2) was 5.71, which reveals that studied population is under the Hardy-Weinberg equilibrium (χ2<5.991). The number of effective allele (ne) was 1.92. It showed that Pasundan bLHR/HhaI had two common alleles. Based on the sequencing analysis results of 46 samples, it showed that new mutation at g.1626G>T was only detected in one individual sample. Polymorphism of bLHR/HhaI gene in the present study was not significantly associated with S/C of Pasundan cows, since the genotype TT of the studied animal was found in the lowest of S/C value than other genotype.
 

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Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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