Background: Cucumber mosaic virus (CMV) is one of the widest spread viruses in the world and has the largest host range of plants.
Aim: The present study was conducted to identify four Saudi CMV isolates (cucumber, tomato, pepper and watermelon) using biological, serological and molecular assays.
Methods: Survey of some vegetable crops exhibiting mosaic, yellowing, blisters, shoestring leaf, mottling and stunting in different regions of Al-Ahsaa at Eastern Province in Saudi Arabia was conducted during the spring of 2016 and 2017. The viral isolates were biologically isolated by single chlorotic local lesion on Chenopodium amaranticolor and propagated in healthy Nicotiana benthamiana. Enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) with specific primers designed for the coat protein gene of CMV were used to detect the virus. RT-PCR products (657 bp) of coat protein gene of CMV were cloned and then sequenced.
Results: CMV was isolated from four vegetable crops (cucumber, tomato, pepper and watermelon) using ELISA and RT-PCR assays and the coat protein gene of the four isolates were submitted to GenBank. The CMV isolates showed nucleotide sequences similarity between them ranged from 97%-99%. Conclusion: This study revealed the existence of CMV in some vegetable crops in Al-Ahsaa at Eastern Province of Saudi Arabia. High nucleotide sequence similarity between the four isolates of CMV suggesting no genetic diversity. The four CMV isolates located in one cluster, belonging to subgroup IA.