Optimum Conditions for Enhanced Production of Podophyllotoxin from Penicillium sp. Isolated from Khanspur, Pakistan
Optimum Conditions for Enhanced Production of Podophyllotoxin from Penicillium sp. Isolated from Khanspur, Pakistan
Umar Farooq Gohar, Attia Majeed, Bushra Muneer* and Hamid Mukhtar
ABSTRACT
Podophyllotoxin, a naturally occurring aryl tetralin lignan produced by few plant species and endophytic fungi is used as precursor for the chemical synthesis of the anticancer drugs. In the present study, for the isolation of podophyllotoxin producing endophytic fungi 30 plant (Podophyllum hexandrum) samples were collected from different localities of Pakistan. About 261 fungal strains isolated, among them 22 strains had the ability to produce podophyllotoxin. Maximum podophyllotoxin production (88.14µg/ml) was shown by fungal strain P-18, which was later identified as Penicillium sp. through 18S rRNA gene sequencing. Optimum growth conditions and parameters such as fermentation medium, pH, temperature, inoculum size, carbon and nitrogen sources were optimized to attain maximum production of podophyllotoxin. The maximum production of 566.23µg/ml was achieved with yeast extract sucrose broth medium with 15% and 5% supplementation of glucose and peptone as carbon and nitrogen source, respectively at pH 5.5 and temperature 25ºC. The endophytic fungi emerging as an alternative source of such medically important secondary metabolites.
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