Identification of Single Nucleotide Polymorphism (SNP) in Exon 9 and Intron 9 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle
Identification of Single Nucleotide Polymorphism (SNP) in Exon 9 and Intron 9 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle
Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana
ABSTRACT
This study aimed to resequencing of 962 bp of exon 9 and intron 9 of follicle stimulating hormone receptor (FSHR) gene in Pesisir cattle to identify single nucleotide polymorphisms (SNP). The samples used in this study were blood samples (n= 70) collected from Pesisir cattle (aged 2-5 years). DNA was isolated and the targeted region was amplified using the polymerase chain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used in this study was polymorphic. There were 5 polymorphisms in the exon 9 and intron 9 regions, which were located at the 18 A>G, 213 T>C, +49 A>T, +232 A>C, and +252 G>A. There were 3 transition type mutations (at positions 8, 213 and +252) and 2 transversion type mutations (at positions +49 and +232). Based on the result of this study, it can be stated that the genotype frequency of Pesisir cattle population was not in the Hardy-Weinberg equilibrium.
Keywords | SNP, FSHR gene, Pesisir cattle, DNA isolation, single nucleotide polymorphism
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