Sequencing and Phylogenetic Analysis of P4b Gene in Pigeon Poxvirus
Sequencing and Phylogenetic Analysis of P4b Gene in Pigeon Poxvirus
Rawaa Saladdin Jumaa
ABSTRACT
This is the first study in Iraq to look at P4b gene analysis of pigeon poxvirus virus in locally infected pigeons with crust lesions in the eye and nostril cere. The DNA extracted from the collected samples was amplified using conventional PCR by amplification of the P4b gene. The positive samples had been inoculated on chorioallantoic membrane (CAM). Thickening of CAM were seen in the first passage, whereas pock lesion at the second (2nd) and third (3rd) passages. The chicken embryo fibroblast (CEF) cells propagated by the prepared infected CAM have shown the cytopathic effect (CPE) that included rounding, detachment of the cells from the monolayer and aggregation of cells during the first and second tissue culture passages. The results of sequencing analysis of pigeon poxvirus field samples revealed that the gene has some mutations (nonsense and missense) and is highly conserved among pigeon poxvirus isolates. The phylogenetic analysis revealed that the P4b gene of Iraqi isolates was related to the (Egypt strain) (99% similarity). Phylogenetic analysis shown that the P4b gene of an avipoxvirus obtained from an infected pigeon was classified as a pigeon poxvirus. The conclude of this study: The main findings that demonstrate the typical characteristic of poxviridae on tissue culture and embryonated chicken eggs. Also, the phylogenetic tree was confirmed that the avipoxvirus infection observed in local pigeons was caused by pigeon poxvirus.
Keywords | Isolation, P4b gene, Sequencing, Pigeon poxvirus, Iraq
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January 2024
Vol. 12, Iss. 1, pp. 1-193
