Coccidiostat and Anthelmintic Activities of Allium sativum Juice: In Vitro Study
Coccidiostat and Anthelmintic Activities of Allium sativum Juice: In Vitro Study
Esam M. Al-Shaebi, Saleh Al-Quraishy, Saleh N. Maodaa, Hossam M.A. Aljawdah and Rewaida Abdel-Gaber*
ABSTRACT
Eimeria species parasite resistance to drugs has been reported, which infects many animals and leads to great economic losses. Medicinal plants are a promising source of a cure for many diseases. This study aimed to investigate the effect of Allium sativum juice (ASJ) on the sporulation of oocytes and as an anthelminthic effector via in vitro study. Characterization of the plant was done by Fourier-transform infrared spectroscopy (FT-IR) and determination of phenolics, flavonoids, and antioxidant activity. The earthworm, Eisenia fetida, is used as a model worm to evaluate the anthelminthic activity of ASJ. The sporulation (%) of Eimeria papillata was assessed by ASJ compared to other detergents. FT-IR showed thirteen active compounds. Our results showed that paralysis and death of earthworms at ASJ (100 %) were 0.14 ± 0.007 and 0.18 ± 0.033 min, respectively, which highly significant than those with the reference drugs. In all treated worms, the histological study revealed obvious surface architecture abnormality. At 72 and 96 h, a high concentration of ASJ (100%) inhibits sporulation by 100%. At 96 h, other concentrations of ASJ (50%, 25%, 12.5%, 6.25%, and 3.12%), as well as amprolium, DettolTM, phenol inhibits oocyst sporulation by 42.29%, 38.02%, 22.44%, 11.63%, 8.50%, 26.66%, 75.45%, and 88.65%, respectively. Results suggest that ASJ possessed strong anthelmintic and coccidiostat properties.
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