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Supplementation of Snakehead Fish Extracts into Tris Egg-Yolk Extender on Mortality and Motility of Limousin Bull Sperm

Supplementation of Snakehead Fish Extracts into Tris Egg-Yolk Extender on Mortality and Motility of Limousin Bull Sperm

Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
 

1Department of Animal Science, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Jl. Raya Tlogomas 246, Malang 65144, East Java, Indonesia; 2Department of Obstetrics and Gynaecology, Faculty of Medicine, Nursing and Health Sciences, Block F, level 5 Monash Medical Centre 246 Clayton Road, Clayton VIC 3168, Monash University, Australia; 3Singosari National Artificial Insemination Center, Ministry of Agriculture Republic of Indonesia, Ngujung, Toyomarto, Singosari, Malang, East Java, Indonesia.


*Correspondence | Aris Winaya, Department of Animal Science, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Jl. Raya Tlogomas 246, Malang, East Java, Indonesia; Email: winaya@umm.ac.id 

ABSTRACT

Freezing process reduces sperm quality due to membrane damage. Antioxidants protects the membrane of spermatozoa. This study aimed to observe the effects of Snakehead Fish (Channa striata [Bloch 1793]) Extract (SFE) supplementation in tris Egg-yolk extender on mortality and motility of spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE. Each of the treatments was repeated three times. The sperm mortality and motility datafrom SFE treatments were analyzed using Analysis of Variance (ANOVA). The results showed no significant effect on the mortality and motility of spermatozoa (P > 0.05) amongst the treatment. However, there was reduction on mortality and tended to increase on the motility of Limousin bull spermatozoa compared to the non-supplemented extender. Furthermore, the SFE can use in egg-yolk extender with some modification for the efficiency of sperm preservation.

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Sarhad Journal of Agriculture

November

Vol.38, Iss. 5, Pages 1-396

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