Morphological and Phylogenetic Characterization of Fasciola species isolated From Cows and Buffaloes in Thi-Qar province, Iraq
Morphological and Phylogenetic Characterization of Fasciola species isolated From Cows and Buffaloes in Thi-Qar province, Iraq
Wesam Jasim Hansh
ABSTRACT
The current study carried out to identify the Fasciola species causing Fascioliosis in cows and buffaloes in Thi-Qar province, Iraq based on morphological measurements and sequences analysis of 28S rRNA gene. Fasciola flukes were isolated from the livers of cows and buffaloes slaughtered in the municipality of AL-Nassiriyah abattoir during July to November 2022. Sixty Fasciola flukes including (30 from cows and 30 from buffaloes) were used in morphological study. Genomic DNA was extracted from 30 Fasciola flukes (15 from cows and 15 from buffaloes) and used to amplified the 28S rRNA gene and amplicons were sequenced. Based on the morphological study and sequences analysis of 28S rRNA gene (618 bp), all Fasciola flukes were identified as Fasciola gigantica with 99 – 100% similarity in the current study when compared with other international samples in the GenBank. 7 sequences of 28S rRNA gene of F. gigentica from cows were placed in the database of the GenBank with accession numbers (LC731384, LC731385, LC731386, LC731387, LC731388, LC731389, LC731390), and 10 other sequences of F.gigentica from buffaloes (LC730609, LC730653, LC730654, LC730655, LC730656, LC730657, LC730658, LC730659, LC730660, LC730661) were also recorded in this study. In phylogenetic analysis, Iraqi F. gigantica samples from cows and buffaloes revealed one distinct clade and clustered with the flukes from different countries of the world, but genetic variation within the F. gigantica cluster showed a considerable distance of F. gigantica with acc. number (LC731390) from cows, and (LC730654) and (LC730655) from buffaloes.
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