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Comet Assay: Quantification of Damaged DNA in Catla catla Exposed to Endosulfan+Chlorpyrifos

Comet Assay: Quantification of Damaged DNA in Catla catla Exposed to Endosulfan+Chlorpyrifos

Huma Naz1*, Sajid Abdullah2, Khalid Abbas2, Muhammad Rizwan Tariq3, Laiba Shafique4, Ghazala Nazeer5

1Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan.
2Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
3Department of Diet and Nutritional Sciences, University of Lahore, Islamabad Campus, Pakistan.
4Animal Sciences and Technology, Guangxi University China.
5Department of Social and Allied Sciences, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan.

Corresponding author: Huma Naz, dr.humanaz98@gmail.com, humanaz@cuvas.edu.pk

 

ABSTRACT

In an acute toxicity trail, LC50 and LC100value (96 hr) of endosulfan (END)+chlorpyrifos (CPF) mixture for Catla catla was computed. The genotoxicity of END+CPF mixture in RBCs of fish was also evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla against END+CPF was computed as 1.35±0.01µgL-1(LC50) and 2.25±0.02µgL-1(LC100). Genotoxic results showed that END+CPF mixture caused significant damage to nuclei (22.58±5.37%) and GDI (0.73±0.15%) in RBCs of fish as compare to control. Aduration-specific damage to DNA was observed in RBCs of C. catla.

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Punjab University Journal of Zoology

June

Vol.39, Iss. 1, Pages 01-134

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