Increased Oxidative Stress and Apoptosis in Splenic Tissue of Lupus-Prone (NZB/NZW) F1 Mice Infected with Live but not Gamma Irradiated Plasmodium chabaudi
Increased Oxidative Stress and Apoptosis in Splenic Tissue of Lupus-Prone (NZB/NZW) F1 Mice Infected with Live but not Gamma Irradiated Plasmodium chabaudi
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
ABSTRACT
Spleen is a major immune organ that plays an important role during malaria infection. Systemic lupus erythematosus (SLE) is an autoimmune disease that affects many body organs including spleen. However, the effect of Plasmodium chabaudi infection on the spleen of the murine lupus model BWF1with the comparison between live and gamma irradiated parasite was not studied before. A total of 30 female BWF1 mice were randomly divided into three groups as follows: group (I) control group (lupus uninfected); group (II) live P. chabaudi infected group (lupus + live P. chabaudi infection); and group (III) irradiated P. chabaudi-infected group (lupus + irradiated P. chabaudi-infection). All groups were killed at day 14 post infection. Histological and biochemical investigations were performed.Live P. chabaudi infection was accompanied with an increase in spleen weight in comparison to either the control or the gamma irradiated P. chabaudi infected group. Differential count of WBCs has revealed an increase in lymphocytes, monocytes and granulocytes count in plasma samples of the live P. chabaudi infected group in comparison to either the control or the gamma irradiated P. chabaudi infected group. Additionally, live P. chabaudi infection has resulted in histopathological changes and increased apoptotic cell death in splenic tissue of infected mice. Moreover, infection with live P. chabaudi was accompanied with an increase in NO, H2O2, and MDA levels in splenic tissue of lupus mice in comparison to either control or gamma irradiated P. chabaudi- infected group. Our data reveal that infection of lupus mice with live P. chabaudi has several effects on the splenic tissue on the histological and biochemical levels. Parasite irradiation with gamma rays can abrogate these effects.
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