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Effect of Glutathione and L-Cysteine Supplementation on the Quality of Frozen Beetal Buck Spermatozoa

Effect of Glutathione and L-Cysteine Supplementation on the Quality of Frozen Beetal Buck Spermatozoa

Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider

Animal Reproduction and Genetics Program, Animal Sciences Institute, National Agricultural Research Centre, PARC, Islamabad 44000

 
* Corresponding author: hammad.fayyax@gmail.com

ABSTRACT

This study was designed to assess the cryosurvival of beetal buck spermatozoa with the supplementation of Glutathione (GLU) and L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS, 0.5mM + 10mM CYS, 1mM GLU + 5mM CYS, 1mM GLU + 10mM CYS and Control). At post-thawing, progressive motility, plasma membrane integrity (PMI), and livability of buck spermatozoa were higher (P< 0.05) with 1mM GLU and 10mM CYS as compared to control. In the second experiment, post-thaw progressive motility, PMI, and livability were higher (P< 0.05) with 1mM GLU + 10mM CYS and 1mM GLU + 5mM CYS compared to other doses of GLU+CYS and control. We concluded that optimized supplementation of Glutathione and L-cysteine in TCA extender ameliorated frozen-thawed quality of beetal buck spermatozoa.
 

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Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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