Caprine Prion Gene Polymorphisms in Main Northern Cyprus Goat Breeds
Caprine Prion Gene Polymorphisms in Main Northern Cyprus Goat Breeds
Meryem Betmezoğlu1*, Dilek Arsoy2 and Mahmut Çerkez Ergören3
ABSTRACT
The administration of genetic breeding programs to eradicate transmissible spongiform encephalopathies in goat and sheep is crucial for animal breeding, health and welfare, together with food safety and security. Goat production and indigenous goat breeds (Cyprus Native hair Goat, Damascus) are important for the Cyprus’s agricultural diversity Therefore, the allele frequencies of Caprine Prnp gene variants should be well understood for goat breeding programs in Northern Cyprus. This current study was designed to determine the putative allele frequencies and genotype distributions of Caprine Prnp gene variations in Northern Cyprus Damascus, Cyprus Native Hair and hybrid goats. Four different locations in the Northern Cyprus were selected for targeted gene sequencing. Blood samples were collected from 50 Northern Cyprus goats. Genomic DNA was extracted from goats’ whole blood. As a result, ten different Caprine Prnp polymorphisms were detected including P42= (CCG>CCA), G127S (GGC>AGC), S138= (AGT>AGC), N146S (AAT>GAT), R154H (CGT>CAT), Gln172Arg (CAG>CGG), G172H (CAG>CAT), G172R (CAG>CGT), V179V (GTG>GTT), V187 (CAG>CAT). The three most common genetic markers were P42= 16%), R154H (12%), N146S (8%), respectively. Two different polymorphism, P42= and R154H, were detected in Cyprus native hair goat whereas P42=, G127S and N146S were observed in the Damascus breed and P42=, G127S, R154H and N146S in Hybrid goats. The predisposition genotype, 146NN homozygosity, was found at the same ratio as the Northern Cyprus Native Hair Goat. There were no statistically significant differences between the breeds. The use of Cyprus Cyprus native hair goat in animal husbandry has a critical importance in terms of species genetic diversity We believe that this breed will play a key role in the reduction of scrapie cases, based on its resistance genotype H154 ratio. Larger studies are needed to gain a complete understanding with regards to the impacts of the 146D, 146S, and 154H alleles in Damascus, Cyprus Native Hair Goat and Damascus-Saanen hybrids.
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