Bicinchonic Acid Assessment for Improvement of Sperms Mitochondrial Functions by Ginseng Extract and Nanoparticales in Rats
Bicinchonic Acid Assessment for Improvement of Sperms Mitochondrial Functions by Ginseng Extract and Nanoparticales in Rats
Noor A. Namaa*, Hayder A.N. Al-Zamely
ABSTRACT
The purpose of this study was to evaluate the impact of Ginseng extract and Ginseng nanoparticles on the level of total protein in testicular tissues utilizing the two assays of Western blotting and Bicinchonic Acid. A total of 60 Wister rats were chosen, prepared, and divided equally into three groups: T1, T2, and negative control, which received only distilled water daily and no other treatment. T1 was given a daily dose of (500 mg/kg) of Ginseng extract, while T2 received a daily dose of 250 mg/kg of Ginseng NPs. After a 60-day experimental period, all study animals were put to sleep using chloroform to collect testis samples, which were then homogenized and measured for protein content with a focus on the SDHA proteins. The results showed that, when compared to the value of the control group, the total protein concentration increased significantly in the Ginseng extracts and Ginseng NPs groups. However, there was no discernible difference in the values of the Ginseng extract or Ginseng NP groups. When compared to the control group, the Ginseng extract and Ginseng NP groups showed increased mitochondrial expression of the SDHA protein. Comparison of the values of the therapeutic groups showed that the Ginseng NP group had higher values than the Ginseng extract group. In conclusion, Western blotting proved to be an effective method for identifying and characterizing specific proteins. Additionally, BCA demonstrated a highly sensitive and straightforward immunoassay for detecting elevated complete testicular protein. However, isolating the active components in Ginseng and creating unique NPs based on one of these components can result in the development of novel medicines with distinctive properties. Additionally, there is a clear need for greater research on the enhancement of Ginseng NPs and correct regulation of genes associated to steroid genesis and mitochondrial biogenesis.
Keywords | Succinate dehydrogenase, Ginseng xtract, Bicinchonic Acid Assay , Sperms, Nanoparticales, Rats
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March 2024
Vol. 12, Iss. 3, pp. 392-585
