Isolation of salivary gland hypertrophy virus in wild populations of the house fly Muscadomestica from Egypt
Isolation of salivary gland hypertrophy virus in wild populations of the house fly Muscadomestica from Egypt
Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*
ABSTRACT
The MdSGHV was isolated from house flies population around the
world. We aimed in this study to isolate this virus from house fly
population in Egypt. Screening house fly population using a new method
based on homogenizing large number of house fly adults collected from
field, clarify and concentrate the filtrate by centrifugation with 1400 g
and 28000 g respectively, resuspend the pellet in Tris buffer, sterile by
0.45-μm syringe filter and inject to house fly adults maintained in
laboratory colony, leads to the detection and isolation of this virus from
Egypt. Injection of filtrate induced SGH symptom in 60% injected flies.
The salivary gland symptom was similar to the previously reported
symptoms of the MdSGHV. Examination of the SGH suspension with
Electron microscopy revealed the presence of rod-shape virus particles
with 500-700 nm in length and 50-70 nm in diameter. PCR analysis of
SGH homogenate with virus specific primer pair resulted in DNA
fragment with expected size as previously mention in literature. These
data demonstrate the presence of the salivary gland hypertrophy virus in
house fly population in Egypt which provides the opportunity to evaluate
the potential use of this virus to control the house fly population in Egypt
within the frame of integrated pest management approach.
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December 2013
Vol. 10, Pages 1-171
