Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats
Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats
Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2
ABSTRACT
Helicobacter pylori (H. pylori) is one of the most prevalent infectious agents in the world which causes a variety of gastrointestinal diseases including gastritis, peptic ulcer, and gastric carcinoma. Laboratory diagnosis of H. pylori infection is made by invasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable and unacceptable for most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. The objective of this study was to comparatively evaluate noninvasive enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the diagnosis of infection with H. Pylori in human stool and fasces of dogs and cats. Two hundred stool samples from humans and eighty-eight fecal samples from dogs and cats were collected with gastric disorders. The presence of H. Pylori infection in stool and fecal samples were tested by ELISA and PCR methods. In ELISA the test utilizes H. pylori antibodies to selectively detect H. pylori Antigen in human stool and fasces of pet animals, and PCR by using 16srRNA primers through a semi-nested PCR technique.
Keywords | ELISA, PCR, 16srRNA
To share on other social networks, click on any share button. What are these?
