An Inhibitory Activity of Propolis Extract against Faba Bean Mottle Bromovirus (BBMV)
An Inhibitory Activity of Propolis Extract against Faba Bean Mottle Bromovirus (BBMV)
E.F. Mohamedl and A.A. Owayss2
ABSTRACT
A Bromovirus was isolated from naturally infected faba bean plants in Fayoum Governorate. The virus was identified as Faba bean mottle Virus (BBMV) according to symptomatology on Faba bean plants, host range, diagnostic hosts, virus stability and ELISA detection. The host range of the isolate was restricted to Fabaceae. BBMV lost infectivity after 10 min at 95 on dilution of 10-3 and after 21 days storage at room temperature. BBMV was easily transmitted by sap. ELISA was used efficiently to detect BBMV in leaves of infected Faba bean plants. The present study was carried out to determine the effect or propolis on BBMV. In vitro experiment, propolis reduced the occurrence of mosaic symptoms on Faba bean plants to 80, 70, 60, 75 and 90% of infected plants immediately: to 75, 65, 45, 55 and 80% after one hour storage period and to 65, 50, 35, 45 and 70% after two hours. when used at a concentration of l, 3, 5, 10 and 20% respectively. In vivo experiment, using of propolis immediately pre-inoculation was more effective in reducing the occurrence of symptoms in infected plants than post-inoculation. The same result was obtained after 1 and 2 hours. So. pre-inoculation treatment was more effective in reducing the occurrence of mosaic symptoms on Faba bean plants infected with BBMV than post-inoculation. The best concentration of propolis used to inhibit the virus was 5%.
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