Genetic Diversity in Bibrik Sheep of Pakistan Elucidated through Molecular Characterization
Genetic Diversity in Bibrik Sheep of Pakistan Elucidated through Molecular Characterization
Jameel Ahmed1,2*, Mohammad Masood Tariq1, Nadeem Rashid1, Asim Faraz3*,
Majed Rafeeq1, Irfan Shehzad Sheikh1, Mudassar Jahan1,2, Abdul Fatih1,2,
Masroor Ahmad Bajwa1, Ifrah Jameel1, Muhammad Ali1 and Saira Iftikhar4
ABSTRACT
Bibrik sheep is found vastly in Baluchistan province in Pakistan. Randomly sampled animals of this breed were subjected to genetic analysis using ovine molecular markers where fifteen sheep SSR genetic markers were employed for describing multiple-allelomorphism. The average observed number of alleles (Na), effective number of alleles (Ne) and Shannon’s Information Index were 2.07 ± 0.79, 1.86 ± 0.69 and 0.54 ± 0.29, respectively. Heterozygosity was described by the average, observed and expected heterozygosities whose values were found to be 0.56 ± 0.27, 0.44 ± 0.27 and 0.43 ± 0.26, respectively. Slightly higher magnitude of observed heterozygosity indicated that forces affecting Hardy-Weinburg Equilibrium were not much effective in these flocks and outbreeding was common phenomenon that reduced the inbreeding in the flocks as inbreeding tends to promote homozygosity. The range in observed heterozygosity was very wide for different molecular markers. These measurements underlined the absence of inbreeding in the flocks that commonly causes inbreeding depression. The presence of genetic diversity was apparent and these markers would be employed for identification of Bibrik sheep through DNA fingerprinting. It was concluded that present assessment of genetic diversity could be used as tool for making future breeding plans but large scale enumeration is required for the Bibrik sheep.
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