In-vitro and In-vivo Fertilizing Potential of Kamohri Buck Semen Frozen in Tris-Based Egg Yolk Extender Supplemented with Selenium
In-vitro and In-vivo Fertilizing Potential of Kamohri Buck Semen Frozen in Tris-Based Egg Yolk Extender Supplemented with Selenium
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
ABSTRACT
To assess the influence of selenium on semen cytological parameters, 48 ejaculations were collected from 4 Kamohri bucks using an artificial vagina twice a week. Following the semen collection, ejaculates were assessed through macroscopic and microscopic evaluation. Semen ejaculates having ≥ 80% motility, morphology, and live-dead ratio were pooled and diluted in Tris-based Egg Yolk (TEY) extender supplemented with various concentrations of selenium, i.e., group A control (0mM), group B (2mM), group C (4mM) and group D (6mM). Results showed significantly (P<0.05) higher motility (78.1±0.22), morphology (86.5±1.08), membrane integrity (85.6±0.30) and live dead ratio (85.3±0.37) for chilled semen in group B than the other groups. Significantly (P<0.05) improved post-thawed motility (68.1±0.42), morphology (73.5±0.3), membrane integrity (70.8±0.36) and live dead ratio (77.1±0.41) were observed in group B as compared to other groups. For in vivo fertility assessment, twenty goats were selected and divided into two experimental groups, viz., group A and B (n=10/group). The goats of both groups were synchronized using the Ovsynch protocol. Group A goats were inseminated with semen extended in a TEY extender without selenium supplementation (control) and group B goats were inseminated with 02 mM selenium supplementation. After 45 days of insemination, pregnancy was confirmed utilizing a transabdominal probe and real-time B mode ultrasonography. The results showed that group B had a significantly (P<0.05) higher conception rate (50%) than group A (30%). In conclusion, supplementation of TEY extender with 2 mM selenium improved in-vitro (chilled and frozen-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen.
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