Effect of Neuromedin S (NMS) on Growth Hormone and its Relationship with NMS Induced Testosterone Secretion in Male Rhesus Monkeys (Macaca mulatta)
Effect of Neuromedin S (NMS) on Growth Hormone and its Relationship with NMS Induced Testosterone Secretion in Male Rhesus Monkeys (Macaca mulatta)
Shakeel Ahmed* and Sarwat Jahan
ABSTRACT
Neuromedin S (NMS), an anorexigenic neuropeptide was first discovered in rat brain. It is a ligand for receptor FM4/TGR-1 which is also called as NMU receptor type II (NMU2R). Mainly it is expressed in SCN and involved in regulation of food intake and dark light circadian rhythms. In rodents and higher primates its stimulatory role in HPG axis is reported. Growth hormone (GH) is released from anterior pituitary and directly or indirectly play very important role in regulation of HPG axis. In the present study the pathway of stimulatory role of NMS was investigated in the regulation of HPG axis. For this purpose, after NMS administration plasma testosterone (T) and growth hormone (GH) levels were determined in four normally fed and 48 hours fasted adult male rhesus monkeys.Fifty nmol (50 nmol)of NMS was injected through a cannula affixed in saphenous vein. Blood samples were collected individually 60 minutes before and 120 minutes after NMS administration at 15 minutes intervals. Plasma T and GH concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly (P<0.001) decreased plasma T levels but it did not cause any significant (P>0.05) change in plasma GH levels compared to normal fed monkeys. NMS injection induced a significant increase (P<0.05) in T and GH concentrations compared to saline treated animals suggesting the possible involvement of GH in NMS induced secretion of testosterone. In summary our results suggest that NMS is a positive modulator of HPG axis and pituitary hormones like GH might be playing an intermediate role.
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February 2021
Vol. 53, Iss. 1, Pages 1-400
