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Detection of Antibiotic Residues of Penicillin and Oxytetracycline in Milk

Detection of Antibiotic Residues of Penicillin and Oxytetracycline in Milk

Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5

1Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Outfall Road, Lahore, 54000, Pakistan
2Department of Parasitology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, 54000, Pakistan
3Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, 54000, Pakistan
4Department of Meat Science and Technology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, 54000, Pakistan
5Population Welfare Department, Government of Punjab, Pakistan.
 
Corresponding Authors: Muhammad Ali Raza
 
alirazakh@gmail.com

ABSTRACT

Nevertheless, the discovery and increased production of pharmaceuticals has lead to substantial use of pharmaceutical products that include antibiotics and steroidal hormones. The day by day increase in human population across the globe is leading to the increased need of food production. Due to the excessive use of common antibiotics i.e. penicillin and oxytetracycline, the antibiotic (ABs) residues remain in livestock products like milk of buffalo and cow. It leads to antibiotic resistance in human beings as well as animals and thus lead to the problem for one health (OH). Following were the objectives of the current study. Detection of commonly used antibiotics (penicillin and oxytetracycline) in (i) raw milk of buffalo and cow collected open market, (ii) UHT milk, (iii) pasteurized milk, (iv) bulk tank milk and (v) gawala’s (milk man) milk in Lahore. Kit detection and HPLC methods were used to identify ABs residues. The samples were collected from Lahore. Raw milk of buffalo and cow collected from the open market have shown 23.5% as the highest positive and UHT milk as the lowest positive 8.5%. Thus, it is an indispensable question to be further explored prospectively. 
 
Novelty Statement | The detection of antibiotic residues including penicillin and oxytetracycline in six different categories of milk were categorically studied for the first time in Lahore with the help of bioassay rapid test and HPLC.
 

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Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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