Preparation and Evaluation of Anti-Rabbit IgY Peroxidase and FITC Conjugates
Preparation and Evaluation of Anti-Rabbit IgY Peroxidase and FITC Conjugates
N. A. Sherif, Lamiaa M. Omar, S. M. Shafei and Elham A. El-Ebiary.
ABSTRACT
In this investigation, a trial for preparation of anti-rabbit immunoglobulin-Y IgY peroxidase and fluorescein isothiocyanate (FITC) conjugates was conducted. Rabbit IgG was separated and injected into specific pathogen free (SPF) laying hens, from which their eggs were collected, the immunoglobulin-Y (IgY) was precipitated from the egg yolk of immunized hens by 33% saturated ammonium sulphate solution. The anti-rabbit immunoglobulin-Y (IgY) was purified by using affinity chromatography. The obtained globulins were divided into 2 parts. The 1st one was conjugated with peroxidase and the 2nd part was conjugated with fluorescein isothiocyanate. Both conjugates gave a strong positive result when diluted 1:2500 and 1:50. respectively. The results indicated that they could be used successfully for detection of antigens and antibodies
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