Submit or Track your Manuscript LOG-IN

Preparation and Evaluation of Anti-Rabbit IgY Peroxidase and FITC Conjugates

Preparation and Evaluation of Anti-Rabbit IgY Peroxidase and FITC Conjugates

N. A. Sherif, Lamiaa M. Omar, S. M. Shafei and Elham A. El-Ebiary.

Central Laboratory for evaluation of Veterinary Biologics. Abbasia, Cairo.

ABSTRACT

In this investigation, a trial for preparation of anti-rabbit immunoglobulin-Y IgY peroxidase and fluorescein isothiocyanate (FITC) conjugates was conducted. Rabbit IgG was separated and injected into specific pathogen free (SPF) laying hens, from which their eggs were collected, the immunoglobulin-Y (IgY) was precipitated from the egg yolk of immunized hens by 33% saturated ammonium sulphate solution. The anti-rabbit immunoglobulin-Y (IgY) was purified by using affinity chromatography. The obtained globulins were divided into 2 parts. The 1st one was conjugated with peroxidase and the 2nd part was conjugated with fluorescein isothiocyanate. Both conjugates gave a strong positive result when diluted 1:2500 and 1:50. respectively. The results indicated that they could be used successfully for detection of antigens and antibodies

To share on other social networks, click on any share button. What are these?

Journal of Virological Sciences

July

Vol. 3, Iss. 1

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe