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Mass Rearing of Spodoptera litura using a Semi-Synthetic Diet Based on Wheat Germ and Tomato Paste

Mass Rearing of Spodoptera litura using a Semi-Synthetic Diet Based on Wheat Germ and Tomato Paste

Sumaria Maqsood1*, Muhammad Ali 2, Amna Shoaib3, Shahbaz Ahmad2 and Noor-ul-Ain2

1Department of Environmental Sciences, Kohsar University, Muree, Pakistan
2Department of Entomology, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan
3Department of Plant Pathology, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan
 
* Corresponding author: smaqsood.iags@pu.edu.pk

ABSTRACT

Spodoptera litura (Hübner) (Lepidoptera: Noctuidae), is the threatening pests of many plants including tomatoes. In vitro, mass-rearing of S. litura is essential to attain a large number of insects with good quality for bio-ecological studies and biological control programs. In the current study, a modified diet was formulated and assessed against two diets used for mass rearing of S. litura, and tomato fruits were taken as a natural diet. Results revealed that development attributes based on mass rearing of S. litura on the modified diet (wheat germ + tomato paste) significantly reduced the larval and pupal developmental duration by 30%, while, considerably enhancing eclosion rate, pupation survival rate, and the number of eggs by 20-30% along with the 40-50% reduction in the pupal and larvae mortality rate as compared to the tomato paste diet and chickpea diet. The rearing expenses for 200 larvae on the modified diet (674/Rs) were 2.5 folds less than the expenses for rearing the same number of larvae on chickpea based diet (1749/Rs.) and almost equal to the tomato paste-based diet (669/Rs.). Therefore, a modified diet based on wheat germ and tomato paste is suitable for mass rearing of S. litura for six generations for laboratory and field experimentation.

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Pakistan Journal of Zoology

February

Vol. 55, Iss. 1, Pages 1-500

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