PCR Detection of Nontuberculous Mycobacteria 16s Rrna in Cows and Sheep Subclinical Mastitis
PCR Detection of Nontuberculous Mycobacteria 16s Rrna in Cows and Sheep Subclinical Mastitis
Namaat R Abdulla1*, Abdullah FA2, Ali Abd Kadhum3, Ghanyem HS4, Noor R Abdulla5
ABSTRACT
The primary goal of the present study was to determine the association of NTM with subclinical mastitis in dairy cows and ewes through the following objectives: Estimation of Subclinical mastitis prevalence and Investigation of usefulness of 16SrDNA for rapid PCR detection of NTM in clinical samples. A total of 120 milk and faecal samples were collected from 70 cow and 50 ewes to identify subclinical mastitis cases using The Modified White Side Test (MWST) and16S rDNA-based PCR. The results of MWST revealed that relatively higher prevalence (83%) of SCM was observed in cows followed by prevalence of SCM in ewes (76%). The difference between this two prevalence was not considered to be statistically significant (p >0.05). The highest prevalence of slight (38%) and severe (31.4 %) SCM reactions are observed in ewes and cows respectively. PCR Detection of NTM 16S rRNA in milk samples indicaecal saated that the higher prevalence (22%) of SCM was observed in ewes followed by prevalence of SCM in cows (6%). The difference between this two prevalence was considered to be statistically significant (p <0.05). The role of NTM as acausal agent of SCM was investigated by PCR amplification of NTM 16S rRNA gene in faecal samples of cows and ewes. The higher prevalence (21.7%) of SCM was observed in scows followed by prevalence of SCM in ewes (16%). The difference between this two prevalence was not considered to be statistically significant (p >0.05).
Keywords | 16S rRNA, Mastitis, NTM
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