Antianemic Effect of Cyclosporine Loaded with Chitosan Nanoparticles on Induced Aplastic Anemia in Adult Male Dogs
Antianemic Effect of Cyclosporine Loaded with Chitosan Nanoparticles on Induced Aplastic Anemia in Adult Male Dogs
Essa D Alhtheal1*, Sufian S Salman2, Salah M AL-Kubaisi3
ABSTRACT
The study of aplastic anemia was induced in adult male dogs using benzene. the efficacy of cyclosporine-laden chitosan nanoparticles in treating induced aplastic anemia was evaluated and the effect of cyclosporine loaded with chitosan nanoparticles with their doses alone was compared. The investigation of possible side effects of treatment regimens used. This study aimed to prepare chitosan nanoparticle and cyclosporine loaded on nanochitosan and pure chitosan. Pure chitosan was prepared using the gelation method. The appropriate cross-linking ratio and cross-linking times of 2% (w/v) chitosan CNPS/cyclosporine nanofibers and solution were prepared. It was dissolved in deionized water at a weight ratio of 2G CNPS diluted with 100 ml DW, and mixtures were prepared by stirring at 80 Co for three and adding five drops of acetic acid. The physical properties and chemical composition were characterized using FE-SEM. Preparation of chitosan nanoparticles with sizes ranging from 20 – 50 nanometers. For succession. Bone marrow examination, counting myeloid cells to nucleated red blood cells revealed significant differences (P < 0.05) between the negative control group and the other groups, where the myeloid cells to nucleated red blood cells after induction of aplastic anemia in adult male dogs decreased from 2 on day 0 to 0.61 On day 15 for the positive control group, from 2.13 to 0.8 for the first group, and from 2.1 to 0.3 for the second group and from 2 to 1.07 for the third group and in the induction stage before giving it the treatment compared to the negative control group, respectively. In a hematological examination, the means of RBC count (1012/l) did not reveal differences in the negative control group. They were 6.5, 7.1, and 6.99 in three consecutive periods, respectively, while the positive control group, there were significant differences in RBC count (1012/l) between zero time, 15-day, and 45-day periods, as it decreased from 7.3 to 3.24, then to 2.48, respectively the results of the blood test after a month of treatment confirmed that there was a clear response to the treatment for the three groups compared to the positive and negative control groups.
Keywords | Chitosan nanoparticles, Hematological parameters, Bone marrow analysis, Benzene-induced aplastic anemia, Cyclosporine
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