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Proximate Composition and Minerals Content of Wheat (Triticum aestivum L.) Under Boric Acid Stress via Energy-Dispersive X-Ray (EDX) Fluorescence Based Analysis

Proximate Composition and Minerals Content of Wheat (Triticum aestivum L.) Under Boric Acid Stress via Energy-Dispersive X-Ray (EDX) Fluorescence Based Analysis

Muhammad Adnan1*, Muhammad Nauman Khan2,3, Barkat Ullah2, Faisal Zaman2, Alevcan Kaplan4, Hubert O. Dossou-Yovo5, Sajid Ali Khan Bangash6, Sana Wahab7, Mehreen Ghazal8 and Muhammad Hassan Sarfraz9

1Iqbal Ahamd Qarshi Laboratory, Qarshi Brands Departnment at Qarshi industries (Pvt.), Ltd. Hattar Haripur, Pakistan; 2Department of Botany, Islamia College Peshawar, 25120, Pakistan; 3Biology Laboratory, University Public School (UPS), University of Peshawar, 25120, Pakistan; 4Department of Crop and Animal Production, Sason Vocational School, Batman University, Batman 72060, Turkiye; 5Laboratory of Applied Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi, Benin, P.O Box 1974 Godomey, Benin; 6Institute of Biotechnology and Genetic Engineering, The University of Agriculture, 25130 Peshawar, Pakistan; 7Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan; 8Centre of Plant Biodiversity, University of Peshawar, Peshawar 25120, Pakistan; 9Botnar Institute of Musculoskeletal Sciences, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, OX3 7LD, United Kingdom.

 
*Correspondence | Muhammad Adnan, Iqbal Ahamd Qarshi Laboratory, Qarshi Brands Departnment at Qarshi industries (Pvt.), Ltd. Hattar Haripur, Pakistan; Email: muhammadadnan112255@gmail.com 

ABSTRACT

Wheat (Triticum aestivum L.) is one of the first domesticated crop plants and is the most widely grown crop in the world, both in terms of cultivated area and yield, and wheat is known to be consumed by about two-thirds of the world’s population. The study was carried out to investigate the elemental and proximate composition of wheat against boron induced stress. Greenhouse experiments were conducted in October 2019 under natural light conditions to assess the chemical status of plants under various treatments such as the stress of inorganic boric acid. In the experiment, the pots were filled with sandy loam soil (1 kg). The pots were pre-treated with boron as boric acid (H3BO3) only once before sowing, at the doses of 0 mg/kg (control), 3 mg/kg, 6 mg/kg, 9 mg/kg, 12 mg/kg, 15 mg/kg and 18 mg/kg soil. The different concentrations of Boron (B) were prepared separately by taking a respective amount of boric acid. Pots without supplemented B constituted the control. No additional supplements were applied to the experimental soil. Each pot was irrigated with 100 mL of distilled water with intermittent intervals of 48 hours. All the treatments were replicated three times considering each pot as one replicate. Through EDX analysis, it was noticed that the boron stress treatment exhibited the maximum number of elements detected in (T2, T5, and T6) wheat crops followed by (T0, T1, and T3). A total of 17 elements were detected and observed in wheat including Ca, S, K, Si, Na, Mg, Fe, Al, Cu, Cr, Cl, Mn, B, C, N, Co, and Ni in different treatments of B. It has been determined that the amount of each element varies according to the amount of B applied. Thus, the study performed under B stress helps understand elemental and proximate content in various treatments in wheat plants. 

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Pakistan Journal of Zoology

April

Pakistan J. Zool., Vol. 56, Iss. 2, pp. 503-1000

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