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Assessment of Dietary Requirement of Vitamin E for Rohu, Labeo rohita Fingerlings Fed Practical Diet

Assessment of Dietary Requirement of Vitamin E for Rohu, Labeo rohita Fingerlings Fed Practical Diet

Uzma Zulfiqar1, Mahroze Fatima2, Syed Zakir Hussain Shah3*, Muhammad Afzal1, Shazia Shamas3, Sadia Roshan3, Moazama Batool4 and Muhammad Bilal5

1Fish Nutrition Laboratory, Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad.
2Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
3Department of Zoology, University of Gujrat, Gujrat, Pakistan.
4Department of Zoology, Govt. College Women University Sialkot, Sialkot
5School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, China
* Corresponding author:,


An 8-weeks feeding experiment was performed to study the requirement of vitamin E for rohu fingerlings. Six practical diets were fed to fingerlings including one control diet (E0) and five test diets (E25, E50, E75, E100 and E125) supplemented with graded vitamin E levels including 0, 25, 50, 75, 100 and 125 mg/kg. After the completion of trial, fish growth, vitamin E, antioxidant enzymes activities and fatty acids profile was determined. From the results it was concluded that the graded supplemental vitamin E levels improve the utilization of feed and fish growth. The antioxidant enzymes activities also showed significant results by increasing vitamin E level in the diet. However, an inverse relation was recorded between the contents of liver thiobarbituric acid reactive substances (TBARS) and vitamin E level in the diet. Moreover, analysis of fatty acids profile showed decrease in saturated and monounsaturated fatty acids (MUFAs). While, polyunsaturated fatty acids (PUFAs) were increased in all vitamin E supplemented diets compared to control group. Furthermore, vitamin E level of 80.62 mg/kg was found optimum for maximum growth performance in L. rohita fingerlings, beyond which no increase in growth was observed.

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Pakistan Journal of Zoology


Vol. 55, Iss. 1, Pages 1-500


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