ETV5 is a Putative Differentially Expressed Transcription Factor of Interleukin 1β Under Neuroinflammatory Conditions in Murine Cerebellum
ETV5 is a Putative Differentially Expressed Transcription Factor of Interleukin 1β Under Neuroinflammatory Conditions in Murine Cerebellum
Abeedha Tu-Allah Khan and Abdul Rauf Shakoori*
ABSTRACT
Neuroinflammation is the underlying cause of a number of neurodegenerative disorders. While the current understanding of its molecular mechanisms is continually increasing, the identification and consequently the roles of transcription factors (TFs) in the respective molecular events under neuroinflammatory conditions in distinct brain regions is still lacking. To address the said gap in knowledge, first we have identified the TFs specific to IL-1β a key player in mediating inflammatory process using online databases. Next, we identified the interacting partners of the significantly differentially expressed TFs out of the selected ones, and the mRNA expression changes were studied for both the differentially expressed TF along with its interacting partners. Finally, we explored the expression of these genes from some online available RNA-seq datasets for brain tissue, neurons, microglia and astrocytes. The results showed significant upregulation of Etv5 TF in male mouse brain hippocampus along with a significant downregulation of three of its predicted interacting partners, i.e., Cop1, Det1 and Bcl6b. Besides presenting new targets for neuroinflammation studies, the discrepancies of expression between different brain regions as well as among the genders suggest that more data with respect to intricate brain region resolution along with single cell data could reflect subtle changes in brain microenvironments and individual cells, which can lead to a more precise pathological understanding of the neuroinflammatory process.
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