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Genetic Structure Analysis of Freshwater Fish Cirrhinus mrigala by Mitochondrial COI Gene

Genetic Structure Analysis of Freshwater Fish Cirrhinus mrigala by Mitochondrial COI Gene

Shahid Sherzada1,2*, Muhammad Naeem Khan1 and Masroor Ellahi Babar2

1Department of Zoology, University of the Punjab, Lahore, Pakistan
2Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan
3University of Agriculture, Dera Ismail Khan, Pakistan
 
* Corresponding author: shahid.sherzada@uvas.edu.pk

ABSTRACT

The current study aimed at genetic analysis of by Cirrhinus mrigala using mitochondrial DNA marker cytochrome oxidase I (COI). Genomic DNA isolated from whole blood was used to amplify and sequence a short region of COI gene in mitochondrial DNA. The identification of sequenced samples was done by NCBI (98-99%) and BOLD (99%) databases. The sequence data analysis represented 15 variable polymorphic sites and 4 haplotypes. Mean haplotypes and nucleotide diversity was 0.42 and 0.0018, respectively. The mean intraspecific and intragenric K2P genetic distances were 0.2% and 1.2%, respectively. Rate of transitional and transversional substitution was 16.68% and 4.16%, respectively. The transition/transversion bias value R was 1.25. The negative values of Tajima D test as well as Fu and Li D and F tests supported the process of population expansion with excess of rare alleles. The overall results showed a lack of neutral evolution and low genetic differentiation among populations of C. mrigala.

 

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Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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