Hepatotoxicity Induced by the therapeutic dose of Chlorpromazine and Ameliorative for Saussurea Costus Roots Extract and Effect of Physiological and Histological Liver of Albino Rats
Hepatotoxicity Induced by the therapeutic dose of Chlorpromazine and Ameliorative for Saussurea Costus Roots Extract and Effect of Physiological and Histological Liver of Albino Rats
Nbaa Mutea Abid AL-Alh1, Nuha Hatem Khalaf1, Nagam Khudhair1*, Ahmed Khalid2
ABSTRACT
Chlorpromazine (CPZ) remains a widely used drug in psychiatric practice today. The aim of this study is to investigate the protective role of ethanolic costus root extracr in CPZ-induced functional and histological changes in rats’ liver. The study was carried out on 30 male albino rats, 12-14 weeks old. The Rats were divided into 5 groups, G1 control negative received distilled water 2 ml. CPZ was administered orally at dose 2 mg/kg of body weight. The rats dosed CPZ were divided as follows: G2 a positive control group, received CPZ alone. G3 was given an ethanolic extract of costus at a dose of 1.5 mg/kg, and G4 was administered an ethanolic extract of costus at a dose of 3 mg/kg. G5 was administered an ethanolic extract of costus plant at a dose of 5 mg/kg. Groups G3, G4, and G5 served as a prophylaxis group dosed 1 hour after CPZ administration. Oral doses are given once daily for 4 weeks. The results of G2 showed increased body weights in rats and liver weights (286.250±13.750 Body Weight) (10.525 ± 0.217 Liver Weight). In contrast a decrease in body and liver Weight is observed among control and Prophylaxis groups (G3, G4 and G5) while no significant difference (p>0.05) in liver and body weights were observed between the groups under study. There was a significant (p<0.001) increase in G2 group ALT, AST and ALP compared a significant decreasing (p<0.001) in prophylactic groups in particular a concentration 5mg/kg or G5 group. Histopathological changes of the liver of G2 including acute cell swelling and necrosis in hepatocyte, inflammatory cells infiltration, blood vessels congestion with perivascular inflammatory cells infiltration, bile ducts epithelial hyperplasia. we noticed that administration of ethanolic extract of Saussurea costus root and in resulted in amelioration of the morphological changes in Chlorpromazine treated rats, improved parameters and restored the parameters to near normal compared with group G1. The G5 group that was dosed with the alcoholic extract of the Indian Costus root and in conjunction with the drug Chlorpromazine at a concentration of 5 mg / kg showed higher improvement in the parenchyma of liver tissue’s compared to the other groups. The concluded Data revealed that root of costus extract acted as a hepatic protective agent against the side effects or toxicity induced by Chlorpromazine.
Keywords | Chlorpromazine, Liver, Toxicity, Rats, Histology, Costus
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