Three Bacteriophages SA, SA2 and SNAF can Control Growth of Milk Isolated Staphylococcal Species
Three Bacteriophages SA, SA2 and SNAF can Control Growth of Milk Isolated Staphylococcal Species
Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*
ABSTRACT
Staphylococcus aureus along with other coagulase negative Staphylococci are among the major mastitis causing organisms. Consumers of milk and milk products from mastitic animals are at high risk of foodborne infections. Escalating antibiotic resistance strived us to explore the alternate option to control mastitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined against ten isolates of Staphylococci and one Micrococcus, isolated from five raw milk samples. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. Their sensitivity against three phages indicated broad host range of phages SANF and SA2, and relatively narrow host range of phage SA. Phage SNAF showed an effective growth reduction of S. aureus RP isolate, compared with other bacteriophages. The bacterial challenge test in milk indicated that proliferation of S. aureus was successfully ceased until six hours post infection when applied at and MOI of 100.
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