Immunopathological and Comparative Study of Concanavalin- A and Streptococcus lyophilized Antigen in Immunized Rats
Immunopathological and Comparative Study of Concanavalin- A and Streptococcus lyophilized Antigen in Immunized Rats
Sura Ayed Radam*, Inam Badr Falih
ABSTRACT
Background Concanavalin-A is a plant lectin neither antibodies, nor enzymes which has four binding sites for glucose and act as an antigen-independent mitogen, frequently used to stimulate proliferation of T-cells and activate the immune response. Aims The present study conducted for showing effect of Concanavalin-A, whole killed lyophilized Streptococcus pyogenes antigen(WKLA) on immune response of rat. Methods To achieve this purpose, 20 Albino male rats were divided equally into four groups and immunized subcutaneously(s/c), 1st group was immunized with (100 mg/ml) of S. pyogenes antigen with added equal volume of Freund’s adjuvant, 2 doses, 14 days intervals, 2nd group was immunized s/c with Concanavalin-A Con-A (1.5 mg/ml), 3rd group was immunized with mixed S. pyogenes Ag. and Con-A, 2 doses, 14 days intervals, and 4th group was given Phosphate buffer saline (PBS) as negative control group. Results Analysis of cellular and humeral immunity recorded higher concentration of Immunoglobulin G IgG, Tumer Necrosis Factor alpha TNF-a and Interleukin 10 IL-10 were revealed in 3rd immunized group (40.91 ±0.26, 953.03 ±4.82 and 1223.96 ±10.44) then decline in 2nd and 1st groups as (32.65 ±1.07, 858.42±9.26 and 1223.96±10.44) ( 21.07 ±0.36, 769.38 ±3.64 and 986.73 ±13.48) respectively. Histopathological findings revealed marked lymphoid hyperplasia with obvious perivascular Mono Nuclear Cells MNCs aggregation composed of lymphocyte and macrophage mainly in hepatic tissue of 3rd group with evidence of reactive lymphoid hyperplasia in splenic tissue of 2nd group and periarteriolar, pericortical lymphoid hyperplasia were reported in spleen of 1st group immunized with Streptococcus pyogenes lyophilized antigen. Conclusion This study provide we can conclude that jointly of concanavalin-A and S.pyogenes antigen capable to enhance potential immune response butter than antigen alone.
Keywords | Comparative study, Streptococcus pyogenes, Concanavalin-A, Lyophilized antigen, Immunized rats, Histopathological
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July 2024
Vol. 12, Iss. 7, pp. 1206-1409
