The Effect of Supplementation Lignolitic Probiotic in Rice Straw for Digestibility and Efficiency of Microbial Protein Synthesis using In Vitro Residual Gas Production
The Effect of Supplementation Lignolitic Probiotic in Rice Straw for Digestibility and Efficiency of Microbial Protein Synthesis using In Vitro Residual Gas Production
Indah Prihartini1*, Miftachi Ari2, Manar Fayiz Mousa Atoum3,4, Akhis Soleh Ismail1 and Listiari Hendraningsih1
ABSTRACT
This study aimed to determine the effect of supplementation lignolitic probiotics in rice straw (Oryza sativa L.) on digestibility and efficiency of microbial protein synthesis using in vitro residual gas production and the optimal lignolitic probiotic in rice straw. The materials used were rice straw IR 64 cultivar and lignolitic TPG probiotic. The method used was Randomized Complete Block Design (RCBD) with four levels of treatments and three groups. In addition, a further test was conducted using Duncan’s multiple range due to a significant difference. The treatments were rice straw added by probiotic level which consisted of (P0: 0 %; P1: 0.5 %; P2: 1.0 %; P3: 1.5 %). Also, the in-vitro gas production was observed at 2 h, 4 h, 8 h, 16 h, 24 h, 36 h, 48 h, 72 h, and 96 h incubation period for digestibility and efficiency of microbial protein synthesis using the in-vitro method. The result showed that treatment significantly affected (P < 0.05) gas production, digestibility, and efficiency of microbial protein synthesis (ESPM). The highest gas production, dry matter (DM), organic matter (OM) digestibility, and ESPM were 73.86 mL 500 mg–1 DM; 43.56 %; 48.41 %, and 34.26 g N kg–1 FOM (fermented organic matter) at 96 h incubation respectively. Therefore, probiotic lignolitic improves the digestibility of rice straw lignocelluse.
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August 2021
Agricultural Productivity and Sustainability Improvement in Tropical Region
