Clinicopathological Studies on Brucellosis in Sheep and Goat at Matrouh Governorate, Egypt
Clinicopathological Studies on Brucellosis in Sheep and Goat at Matrouh Governorate, Egypt
Asmaa A. Darwish*, Mona A. Mahmoud, Adel M. El-Kattan
ABSTRACT
Brucellosis is a global zoonotic disease. This work aimed to study the clinicopathological changes in brucella-infected sheep and goat at Matrouh governorate and suggest new markers for brucellosis. Sera were obtained randomly from ewes and does then tested serologically by the Rose Bengal Plate test (RBPT) and competitive ELISA test (cELISA). The animals of each specie were divided into 2 groups: the control group CG (results of both tests were negative) and the diseased group DG (results of one or both tests were positive). The clinicopathological parameters were estimated and statistically analyzed. The brucellosis seroprevalence was (17.50%, 22.50%) for sheep, and (30%, 31.25%) for goat by RBPT and cELISA respectively. Both infected species showed a significant (P<0.05) macrocytic hypochromic anemia, hyperglobulinemia, hypoalbuminemia, decreased A/G ratio, increased hepatic function tests, hypertriglyceridemia, increased matrix metalloproteinases activity, T/HDL/LDL-hypocholesterolemia, and decreased minerals, electrolytes, and trace elements concentrations and these changes were more prominent in goat than in sheep. While, the diseased ewes suffered from higher degrees of leukocytosis, total hyperproteinemia, hypoglycemia, increased kidney function tests, and oxidative stress than the diseased does. Total antioxidant capacity (TAC) and Zn had the highest likelihood ratios among the suggested markers with acceptable sensitivity, specificity, PPV, NPV, and accuracy rates, and their combination yielded better values. The study concluded that the clinicopathological alterations associated with ovine and caprine brucellosis are a mirror for the immune response against the disease. Most of these changes were more prominent in goat than in sheep. TAC and Zn (separated or combined) are excellent biomarkers for ovine and caprine brucellosis.
Keywords | Brucellosis, Clinicopathological alterations, Matrix metalloproteinases, New biomarkers, Seroprevallance
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