Transformation Efficiency of Five Agrobacterium Strains in Diploid and Tetraploid Potatoes
Transformation Efficiency of Five Agrobacterium Strains in Diploid and Tetraploid Potatoes
Betül Ayça Dönmez, Sarbesh Das Dangol and Allah Bakhsh*
ABSTRACT
Development of transgenic potatoes is imperative to investigate various gene functions as well as to develop robust potato varieties resistant to different biotic and abiotic stresses, specially in diploid potato and to redirect the breeding program away from tetraploids to the diploids. We aimed to develop the protocol for transformation of Solanum chacoense diploid M6 potato using tetraploid S. tuberorsum cv. Desiree as a control using five different Agrobacterium strains harboring pBIN19 binary vector that further contains gusA gene, interrupted by an intronic sequence, under the control of 35S CaMV promoter. After transformation, we analyzed the transformation efficiencies of each of the Agrobacterium strains using the histochemical GUS analysis. The highest gusA gene transfer efficiency rate both for leaf (60%) and internode (100%) explants was observed in cv. Desiree using Agrobacterium strain GV2260, while the highest gene transfer efficiency rate for leaf and internode explants in S. chacoense M6 were obtained with AGL1. The highest callus formation for both cv. Desiree and S. chacoense M6 was obtained on cv. Desiree leaf explants, transformed via the Agrobacterium strain GV2260. The lowest callus formation, for both S. chacoense M6 and cv. Desiree, were obtained from internode explants. About 33% calli induction was achieved from transformation mediated by AGL1 strain in cv. Desiree, and about 23 % from LBA4404 strain in S. chacoense M6. This study will further aid in the development of transgenic stable transgenic potatoes, especially in diploid cultivars.
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