Peste des petits ruminants (PPR) is as a transboundary disease whose occurrence hampers the trade and consequently causes an enormous economic losses. It is a disease of sheep and goat, particularly in the regions of Africa, Middle East and Asia. Besides clinical signs and symptoms of PPR various diagnostic assays, both serological and molecular, have been developed and are applied for estimating the current status of the disease. This review article focuses on the development of different molecular diagnostic assays from conventional reverse transcriptase polymerase chain reaction to real time reverse transcriptase polymerase chain reaction and reverse transcriptase loop mediated isothermal amplification over the last decade. These diagnostic assays vary in their sensitivity, specificity, reliability and reproducibility. Although these assays are detecting PPRV with precision but there is need to develop some cost effective diagnostic assays which would be applicable in the field conditions and in the developing countries like Pakistan.
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