The Potential of Using Ocimum gratissimum Leaf Essential Oils as a Supplement in Extender to Improve Chilled Canine Sperm Quality by Assessing Its Antioxidant Effects
The Potential of Using Ocimum gratissimum Leaf Essential Oils as a Supplement in Extender to Improve Chilled Canine Sperm Quality by Assessing Its Antioxidant Effects
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
ABSTRACT
The preservation of dog sperm is impeded by oxidative stress, leading researchers to investigate the impact of incorporating essential oils derived from Ocimum gratissimum leaves in order to prolong the viability of chilled dog sperm. The sperm’s characteristics were assessed using an automated sperm analyzer and a confocal microscope to analyze motility and membrane properties, respectively. Additionally, malondialdehyde production was measured to evaluate lipid peroxidation capacity. The results revealed that incorporating essential oils at concentrations under 100 µg/ml in semen extenders had a positive impact on sperm quality. Nonetheless, employing essential oils at elevated concentrations of 200 µg/ml exhibited detrimental impacts on the sperm. The optimal dosage for maintaining the integrity of the dog sperm’s membrane and enhancing its property parameters was determined to be a supplementation of 100 µg/ml essential oils, which demonstrated a significant difference from the control treatment throughout the 12-day storage period (P<0.05). In summary, the results indicate that the properties of dog sperm during preservation are influenced by the dosage of Ocimum gratissimum leaf essential oils in a manner that depends on the concentration. The most favorable outcomes were observed when using a maximum essential oil concentration of 100 µg/ml.
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