Effects of Zinc Sulfate and Vitamin D3 in Young Male Rats with Zinc Deficiency; Hemogram Criteria
Effects of Zinc Sulfate and Vitamin D3 in Young Male Rats with Zinc Deficiency; Hemogram Criteria
Maryam Ali Mohammed, Ahlam A. Al-Rikaby*
ABSTRACT
The zinc (Zn) is one of the vital elements that are needed for body growth and important physiological processes as well as it improves immune competence and productivity. The current work was undertaken to assess the ameliorating role of zinc sulfate and Vitamin D3 on hematological alterations in male rats with induced zinc deficiency. For this purpose, forty healthy male rats (immature) were randomly divided into two groups; First group (n = 10; control) acted as healthy male rats and received 0.5 ml of dimethyl sulphoxide (DMSO) intraperitonially (I.P). The second group (Zinc deficiency group) consisted of 30 male rats; received single adose of 1,10 phenanthroline 30 mg/kg dissolved in 0.5 ml dimethyl sulphoxide (DMSO) I.P. After 12 h-fasting, zinc deficiency was determined by measuring the serum zinc level after 24 hrs from 1,10 phenanthroline injected. After treatment ,animals were distributed into four groups, 1st group healthy rats acted as negative control treated with physiological saline solution(1ml), 2nd group carried zinc deficiency as positive control treated with physiological saline solution, 3rd group zinc deficiency rats were treated with zinc sulfate 20mg/kg dissolved by physiological saline solution; 4th group zinc deficiency treated with vitamin D 500 IU/kg; The treatment was performed in all groups orally and continued for 30 days. Our findings indicated that zinc deficiency induced hematological changes decreasing erythrogram values in concomitant with increased thrombocytes and leukogram count except LYM value which decreased. In conclusion, zinc deficiency led to moderate anemia whereas the oral administration of zinc and vitamin D corrected the zinc deficiency–induced inflammatory response, consequently improved blood profile erythroproiesis and leukogram.
Keywords | Zinc deficiency, Erythrogram, Leuckogram, Anemia, Young rats
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November 2024
Vol. 12, Sp. Iss. 1
