Phylogenetic Tracking of Ica-locus in MRSA from Different Sources at Ismailia City, Egypt
Phylogenetic Tracking of Ica-locus in MRSA from Different Sources at Ismailia City, Egypt
Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1
ABSTRACT
Tracking of MRSA strains which encoded intracellular adhesion (ica) locus is a major threat for livestock and public health. As it indicates that the isolated strains have ability to biofilm formation leading to zoonotic risk and nosocomial infections. So, this study focused on tracking the ica locus in different hosts and other virulence factors with genetic analysis of recovered isolates. A total of 350 random samples were collected from animals and hospital acquired infected patients. The recovered isolates were identified biochemically by vitek2 compact, confirmed by PCR and tested for sensitivity against varieties of antimicrobials. Three multi-drug resistant isolates carried ica from human abscess (A9), sputum of dog (A12), and cow mastitic milk (A13) were sequenced with other genome sequences retrieved from the GenBank. Ten samples (9.17%) were S. aureus from human, while 3 samples (1.24%) were S. aureus and 2 samples (0.83%) were coagulase negative staphylococcus (CONS) from animal sources. The multiple sequences alignments (MSA) and phylogenetic tracking proved the genetic similarity among A9, A12 and A13 from (human, animal, food of animal origin respectively) reached to 94 %. In conclusion, although the low isolation rate of confirmed MRSA in Ismailia governorate, the three selected MRSA strains invigorate the possibility of transmission among different sources of food, animals, and human, which causes nosocomial infections.
Keywords | Ica-MRSA, PCR, Virulence factors, Resistant factors, Phylogenomics.
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