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Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.

Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

ABSTRACT

Lumpy skin disease (LSD) is one of the most serious livestock diseases in Egypt since 1988 when the disease spread widely all over the country. since that attenuated sheep pox virus (SPV) used for vaccination cattle flocks against LSD due to immunological relationship between LSD and SPV. There have been numerous attempts to produce live attenuated LSD vaccine but it has stopped due to the seriousness of the disease's return to its virulence and the post vaccinal reaction of the vaccine so we prepare inactivated LSD virus(LSDV) vaccine to immunize the cattle with safe vaccine .Ismailia isolate of LSD was propagated and titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 5.5 TCID50/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characterization of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37°c. The inactivated LSDV tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective dose (MPD) of prepared inactivated LSDV. The MPD was 1.5 ml of the inactivated virus and the vaccine was prepared by adding equal volume of the adjuvants (Montanide ISA 50 and Montanide ISA 206 ) to the total quantity 3ml of the vaccine. The prepared vaccine tested for determination of immunological response against the vaccine with ISA 50 and ISA 206 adjuvants and the results was compared. The results of cellular and humoral immune response and the challenge test showed that using inactivated LSDV vaccine improved by Montanide ISA 206 elevate the protective immunity level and considered more safe and avoid viral reversion and transmission of the virus by the blood feeding arthropods.

 

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Journal of Virological Sciences

July

Vol. 8, Pages 1-27

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