Analysis of Polymorphism of Growth Hormone Secretagogue Receptor in Sheep
Analysis of Polymorphism of Growth Hormone Secretagogue Receptor in Sheep
Jun Yan Bai*, Hong Deng Fan, You Bing Yang, Xu Wang, Heng Cao, Xue Yan Fu and Yu Qin Wang
ABSTRACT
The purpose of this study was to find candidate genes regulating the growth and development of sheep. The polymorphism of GHSR gene in five sheep populations was analyzed by PCR and sequencing techniques. The results showed that large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep, hu sheep all have two mutation sites (C155T and C624T). For locus C155T, allele frequencies of C in large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep and hu sheep were 0.73, 0.83, 0.54, 0.69 and 0.68 respectively, which indicated that C was the dominant allele in five sheep populations. For locus C624T, allele frequencies of C in large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep and hu sheep were 0.70, 0.87, 0.52, 0.70 and 0.76 respectively, which indicated that C was the dominant allele in five sheep populations. The C155T mutation site of GHSR-3 gene led to a codon change from GCC to GCT, both of which coding the same aa, alanine, indicating C155T was a synonymous mutation site. The C624T mutation site of GHSR-4 gene led to a code change from CTT to CCT, and the corresponding aa changed from leucine to proline, which was a missense mutation site.
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June 2020
Vol. 52, Iss. 3, Pages 825-1224
