Comparison Between Molecular and Traditional Methods of Diagnosing Trypanosoma evansi in Camels
Comparison Between Molecular and Traditional Methods of Diagnosing Trypanosoma evansi in Camels
Qasim Jawad Amer, Wissam Ahmed Sabah Al-Janabi*
ABSTRACT
Trypanosoma evansi (T. evansi) is a significant pathogen affecting camels arround the globe. The study aims to evaluate the potential of molecular methods as a reliable alternative to traditional techniques for accurate and rapid diagnosis of T. evansi in camel populations. For this purpose, this study was designed to collect seventy-five blood samples (n=75) from camels of different ages of both sexes (male and female) from different areas of northern and southern Babylon.Each blood sample was placed in a special anticoagulant tube (EDTA), with a label containing the number, age, and sex, and tProcessed for analysis by microscopic examination and confirmed by PCR. The data analysis showed that among 75 camels, 17% were females, 73% were adults, 67% from north Babylon.Analysis of microscopic and molecular examination identified positive samples in 9% and 57%, respectively. Comparative analysis between microscopic examination and PCR methods showed had high rate of infection (43/75, 57.33%), while the microscopic examination showed a low rate of infection (9.2%) (7/75), with significant difference of T. evansi infection (P< 0.00001). Taken together, we noticed that microscopic diagnostic techniques were less sensitive than molecular techniques, indicating that microscopic techniques may provide false negative results. This justify the application of molecular techniques in diagnosis of T.evansi for accurate dentificationn and characterizaiton of infection in camels.
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