A survey of District Hunza, Gilgit and Nager, Gilgit-Baltistan, Pakistan was conducted during January, 2017. Out
of 20 soil samples, 6 samples were found positive for Steinernema species. One sample of Prunus avium had
Steinernema affine from Hunza, two samples had S. cholashanense from Juglans nigra and Malus pumila from
Gilgit, and three samples harboured S. feltiae from Prunus armeniaca, Prunus persica and Malus pumila from
Nager. This paper deals with re-description of S. affine based on comparative study of morphological and
molecular characteristics with brief study of S. cholashanense and S. feltiae. S. affine Pak. G.S.356 deposited in
NCBI with accession number MF150033; S. cholashanense Pak.G.S.350 and Pak. G.S.355 with accession
numbers MF125282 and MF0339642, respectively; S. feltiae Pak. G.S. 354, 357, 358 deposited with accession
numbers MF150034, MF158314 and MF144570, respectively.