Profile of Melanocortin-4 Receptor and Myogenic Factor 5 Genes in Pasundan Beef Cattle
Profile of Melanocortin-4 Receptor and Myogenic Factor 5 Genes in Pasundan Beef Cattle
Amaq Fadholly1,2*, Endang Tri Margawati1, Alek Ibrahim1 and Widya Pintaka Bayu Putra1
ABSTRACT
Melanocortin-4 receptor (MC4R) and myogenic factor 5 (MYF5) play an important role in the growth trait, including regulating myogenesis. The study aimed to examine the polymorphism of MC4R and MYF5 genes as a reference for selecting growth traits in Pasundan beef cattle. Amount of 50 blood samples was collected from Breeding Center (?) of BPPT Cijeunjing, Ciamis, West Java, Indonesia. DNA samples were isolated and then purified following the extraction continued with polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP). The Association of MYF5 genotypes with body weight and body size measurements was analyzed using General Linear Model by SAS 9.4 program. The result showed that MC4R gene with HpyCH41V restriction enzyme was polymorphic with three genotypes of CC, CG and GG with frequencies of 0.18 (9), 0.32 (16) and 0.5 (25), respectively. The MY5 gene also showed three genotypes of AA, AG and GG with frequencies of 0.08 (4), 0.38 (19) and 0.54 (27), respectively. Association of the polymorphism both MC4R and MYF5 genes indicated highest body weight with GG genotype. Both of these genes could be associated with growth trait, namely body weight markers. Thus, Further study needs to be conducted in association with more related genes for groth trait to get more comphrehensives results then can be applied for local Indonesian beef cattle.
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