In the last decade, bisphenol A (BPA) has received heightened attention because of its ubiquitous presence in our living environment. However, it was revealed that this component has potent genotoxicity, causing various disorders to human and animal health. Hence, the present work is designed to investigate the capacity of Fagonia cretica extract (FCE) to inhibit bisphenol A-induced genotoxicity and histopathology in rats. In the genetic study, the assaying of expressions of IkBα and COX-1 genes in liver tissues, as well as the expressions of mdr1a and COX-1 genes in kidney tissues are evaluated. In addition, the histological architectures of these organs are examined. Bisphenol A (BPA) treatment causes over-expressions of the above mentioned genes, and induces massive damage to the histological architectures of liver and kidney tissues. In contrast, FCE treatment with different doses (3.3 g/Kg., 4.2 g/kg. and 5.0 g/kg) can inhibit the upregulation of such gene expressions and enhance the histopathological changes of liver and kidney organs. These ameliorations increase by increasing the dose of FCE, through its utilization either as a protective or therapeutic agent. Using FCE as a therapeutic agent, particularly in the treatment with the highest dose (5.0 g/Kg), produces the best results, where some genotoxic and histopathological parameters are restored to the normal level or natural status. The present investigation confirms the important role of Fagonia cretica in overcoming the harmful BPA-induced effects on animal cells, since the extraction of this medicinal herb can modulate the over-expressions of IkBα, mdr1a and COX-1 genes to favorable or normal levels in rats. Moreover, it may be able to markedly ameliorate or remedy the histopathological cases.
Keywords | Fagonia cretica, Bisphenol A, Gene expression, Histopathology, Rats