Sequencing and Phylogenetics of the Cytochrom B Gene in Bali Cows With Different Coat Color in Kupang, East Nusa Tenggara, Indonesia
Sequencing and Phylogenetics of the Cytochrom B Gene in Bali Cows With Different Coat Color in Kupang, East Nusa Tenggara, Indonesia
Arnold Christian Tabun*, Ferdinan Suharjono Suek, Cardial L Leo Penu, Johanis A. Jermias, Thomas Lapenangga
ABSTRACT
Bali cows are native Indonesian cattle reared by farms in Kupang with varying coat color. The research objective was to determine the Bali cows with different coat color in Kupang district based on DNA analysis using the cytochrome b (CYT B) gene. The methods used CYT B gene base methods of DNA analysis such as DNA extraction, Polymerase Chain Restriction, DNA sequencing, and identification of DNA sequences nucleotide. Bali cows with sorrel, black and white coat colors are used with an age range of 3-5 years. DNA sequencing conducted multiple alignments, the identification of the genetic distance, and the preparation of phylogenetic trees using the software Mega X. The results of PCR sequencing of Bali cows with different coat colors in Kupang were 464 bp. Identification of single nucleotide polymorphism in Bali cows compared to Bos taurus and Bos javanicus as many as 29 SNPs. The percentage of nucleotide similarity with Bos taurus was 93.75% and the nucleotide diversity was 6.25%. The percentage of nucleotide similarity with Bos javanicus is 100%. The conclusion is that Bali cows with different coat colors in Kupang have 100% genetic similarity with Bos javanicus and the CYT B gene can be used as a genetic marker for Bali cows with different coat colors in Kupang.
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