Comparative Study on Two Thrips Transmitted Viruses Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) as a Tospovirus
Comparative Study on Two Thrips Transmitted Viruses Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) as a Tospovirus
Manal A. El-Shazly1, A. s. 2 Abdel Wahab and Salwa N. Zein3
ABSTRACT
Biological, biochemical and serological comparison between Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) a Tospovirus were studied These two viruses were isolated from Vinca rosae (L). G. Don and onion (Allium cepa L.), respectively. The observed symptoms included systemic mosaic and yellowing for TSWV and necrotic eyelike spots and yellow spots on leaves, and flower stem for IYSV. Both TSWV and IYSV were mechanically and seed transmitted. TSWV was transmitted by two different thrips species, Thrips tabaci L (33.3%) and Frankliniella occidentallis Pergamde (60.9%) whereas the transmission of IYSV was obtained by Thrips tabaci L. only (45%). Adults of T. tabaci and F. occidentallis Pergamde as vectors of TSWV and IYSV were discussed. Franklinella tritici L. and Gynaikothrips ficorum Marchal did not play a role as vectors of these plant viruses. Both TSWV and IYSV had a wide host range the differences in host reactions were studied. TSWV differed in its stability properties from IYSV in dilution end point (DEP) and longevity in vitro (LIV) but the two viruses are heat-inactivated at 55 oc. Purified TSWV and IYSV each migrated as a single zone in density gradient column. Ultraviolet absorbance of both TSWV and IYSV were typical of nucleoprotein with minimum and maximum at 247 and 260 nm for TSWV and IYSV, respectively. The ratios of A260Q80 and A were 1.2 and 1.11 for TSWV and 1.2, 1.3 for IYSV. Electron microscopy of purified TSWV and IYSV showed the presence of spherical panicles with 85 nm and 80-120 nm in diameter for TSWV and IYSV, respectively. Titer of die prepared antisera as determined using indirect ELISA were 1/3000 and 1/8000 for TSWV and IYSV, respectively. Authentic and induced antisera for both TSWV and IYSV were used for virus detection using different serological diagnostic methods such as indirect ELISA and dot-blot immunoassay (DBIA) on nitrocellulose membranes.
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