Genetic Analysis of African Swine Fever Virus Originating from Pork Products in Indonesia
Genetic Analysis of African Swine Fever Virus Originating from Pork Products in Indonesia
Seruni Agistiana1, I. Wayan Teguh Wibawan2, Ni Luh Putu Ika Mayasari2, Harimurti Nuradji3, Surachmi Setiyaningsih2*
ABSTRACT
African swine fever (ASF) is a viral pig disease that has spread internationally and has a severe impact on pork production in the agricultural sector, trade economy, and social welfare in affected regions. In this context, pork products can potentially be a source of the spread owing to the long persistence of the causal virus. Therefore, this study aims to analyze ASFV genetically from pork product samples in 2019-2023 based on the B646L gene and determine the kinship relationship with reference ASFV in Indonesia and other countries. The study used 38 pork products collected in 2020-2023, consisting of 28 archived samples of ASFV positive, which were imported or transported between domestic regions. The other 10 were imported and local pork products purchased at e-commerce stores to represent samples that were already circulating in the community. Subsequently, the isolates were sequenced and compared with other ASFV isolates to establish the virus genotype. Sanger sequencing was conducted using the B646L gene, which was the most frequently used genetic marker. The phylogenetic tree construction results showed that genotype II was present in the samples, which was similar to Indonesian isolates and other Asian isolates. Therefore, the import of pigs and pork products must be regulated to prevent the entry of other genotypes and wider domestic spread.
Keywords | African swine fever, B646L, Gene, Indonesia, p72, Pork product
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