Quality of Aceh Cattle Ovaries Added with Bovine Pituitary Extract in the Vitrification Medium
Quality of Aceh Cattle Ovaries Added with Bovine Pituitary Extract in the Vitrification Medium
Cut Intan Novita1,2, Kartini Eriani3, Amalia Sutriana4, Tongku Nizwan Siregar5,6*, Ni Wayan Karja7
ABSTRACT
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) hormones are often added to ovarian vitrification media to prevent apoptosis of granulosa cell follicles. Bovine pituitary has been proven as an effective substitute for the functions of FSH and LH in some studies related to superovulation due to its high content of both hormones. Therefore, this study aimed to determine the effect of bovine pituitary extract (BPE) supplementaion to vitrification media. A total of 18 ovaries were usedwhich was divided into two groups: the vitrification medium group without Bovine Pituitary Extract (BPE, GibscoTM) (1 ml PBS + 0.5 M sucrose + 30% ethylene glycol) and with BPE addition (1 ml PBS + 0.5 M sucrose + 30% ethylene glycol + BPE 30 μg/mL). Each group was separated into three vitrification times of 0, 7, and 14 days with three replications. The results showed that adding BPE to the vitrification medium increased the number of intact follicles (P<0.05) at each development stage, with the highest number occurring in the primordial and primary follicle stages. The number of primordial and primary follicles on day 14 of vitrification the control group vs the BPE group was 62.08±18.25 vs 72.72±5.67 and 45.62±15,9 vs 65.78±7.17, respectively. In conclusion, the addition of BPE to the vitrification medium could increase the number of intact follicles, preventing damage that may occur during the vitrification process.
Keywords | Aceh cattle, Bovine pituitary extract, Follicle, Reproductive technology, Vitrification
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April 2024
Vol. 12, Iss. 4, pp. 586-801
