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Prevalence of H9N2 Avian Influenza Viruses in Hazara Region of Pakistan

Prevalence of H9N2 Avian Influenza Viruses in Hazara Region of Pakistan

Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2

1Poultry Research Institute, Jaaba Mansehra, Khyber Pakhtunkhwa, Pakistan
2NRLPD, National Agricultural Research Centre, Islamabad, Pakistan
3Veterinary Research and Disease Investigation Centre, Abbottabad, Khyber Pakhtunkhwa, Pakistan

*     Corresponding author:


A surveillance of avian influenza viruses in Hazara region of Khyber Pakhtunkhwa Pakistan was carried out from April 2013 through September 2014. Pooled morbid material and fecal samples from 600 different locations across the region were analyzed for isolation. Isolation was carried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase polymerase chain reaction (RT-PCR) procedure. Out of 600 examined locations twenty two (22) H9N2 subtypes isolates were recorded. The total prevalence recorded was 3.66%and out of 498 broiler operations yielded 17 (3.4%) positive isolates and 44 golden type native chicken farms yielded 05 (11.36%) isolates of AIV H9N2 and 58 captive pheasant’s samples were negative. It was observed that frequency of H9N2 prevalence in poultry population was directly proportional to temperature, season and elevation.

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Pakistan Journal of Zoology


Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500


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